CD28 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	CD28
Entrez Gene ID	106549457
Full Name	CD28 molecule
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014067118.1	XP_013922593.1	T-cell-specific surface glycoprotein CD28

Pathways from BioSystems
Gene Ontology

The following CD28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh11886	XM_014067118.1 Latest version!	Thamnophis sirtalis CD28 molecule (CD28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD28 ( XM_014067118.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013922593.1 Thamnophis sirtalis CD28 molecule (CD28), mRNA.

Price & Availability↑

CloneID	OTh11886
Clone ID Related Accession (Same CDS sequence)	XM_014067118.1
Accession Version	XM_014067118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	T-cell-specific surface glycoprotein CD28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATTG TGCCTTTTAC CGGCATAATC GCCTGGATTG TTCTTACTGT CGTTCATATT 
ATTCAGGACA CCAGTTCAGC AGCTGAAAAC ATTTCAAAGC AACAGAGAGT CCTAAACAAA 
ACAGTGCACC AAAATGCCCA GCTGGCCTGT GACTTTCCAG CTCACAGAGA AACCATCACA 
AAGTTGAATG CCTACTTGTT CAAAGGAGTC GGGAAAGTAC AACTTTTTGA AGCCAATTGG 
AACAATTCCA CAATTTCAAT CAAGGAAAAC GGTTCTAAAT TAAGCTTGGA CATAAATATT 
ACAAAAGAAG AAAAACATGC TGTGTTTCAC CTGAAGGACT TGGAAGTCAA CCACACAGAC 
AGTTACATGT GCAAAATTGA GGTGATTGAC CCGCCACCAT ATGAAACCGA AAGCAGGAGC 
AACTTAATTT ACGTGACAGA ACTTCCTCCG CACCAAGAAA AGCTTAATTG CATGAACATG 
CCAATGGCTA TTTCACTTGG ATTTTTCGTT TTCTACAGCC TGATGATCAC AGCAGCCATT 
TACTACTGTT GGCTGAAAAG CAAGAAGAAT AGGGCCCTTC AGAATGACTA TTTCAATATG 
ACGTCCTGGC AATCAAATGG ACCCAGGAAA AGGCCGCCTC GGCATGGTGC TCCAGCACGG 
AACTATACGG CATACCGTTA TTGGGAACCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013922593.1
CDS	159..851
Translation	MPIVPFTGIIAWIVLTVVHIIQDTSSAAENISKQQRVLNKTVHQNAQLACDFPAHRETITKLNAYLFKGVGKVQLFEANWNNSTISIKENGSKLSLDINITKEEKHAVFHLKDLEVNHTDSYMCKIEVIDPPPYETESRSNLIYVTELPPHQEKLNCMNMPMAISLGFFVFYSLMITAAIYYCWLKSKKNRALQNDYFNMTSWQSNGPRKRPPRHGAPARNYTAYRYWEP

Target ORF information:

RefSeq Version	XM_014067118.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis CD28 molecule (CD28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014067118.1

ORF Insert Sequence:

ATGCCCATTG TGCCTTTTAC CGGCATAATC GCCTGGATTG TTCTTACTGT CGTTCATATT 
ATTCAGGACA CCAGTTCAGC AGCTGAAAAC ATTTCAAAGC AACAGAGAGT CCTAAACAAA 
ACAGTGCACC AAAATGCCCA GCTGGCCTGT GACTTTCCAG CTCACAGAGA AACCATCACA 
AAGTTGAATG CCTACTTGTT CAAAGGAGTC GGGAAAGTAC AACTTTTTGA AGCCAATTGG 
AACAATTCCA CAATTTCAAT CAAGGAAAAC GGTTCTAAAT TAAGCTTGGA CATAAATATT 
ACAAAAGAAG AAAAACATGC TGTGTTTCAC CTGAAGGACT TGGAAGTCAA CCACACAGAC 
AGTTACATGT GCAAAATTGA GGTGATTGAC CCGCCACCAT ATGAAACCGA AAGCAGGAGC 
AACTTAATTT ACGTGACAGA ACTTCCTCCG CACCAAGAAA AGCTTAATTG CATGAACATG 
CCAATGGCTA TTTCACTTGG ATTTTTCGTT TTCTACAGCC TGATGATCAC AGCAGCCATT 
TACTACTGTT GGCTGAAAAG CAAGAAGAAT AGGGCCCTTC AGAATGACTA TTTCAATATG 
ACGTCCTGGC AATCAAATGG ACCCAGGAAA AGGCCGCCTC GGCATGGTGC TCCAGCACGG 
AACTATACGG CATACCGTTA TTGGGAACCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.