ELN cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	ELN
Entrez Gene ID	106548240
Full Name	elastin
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014065579.1	XP_013921054.1	elastin

Pathways from BioSystems
Gene Ontology

The following ELN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh23972	XM_014065579.1 Latest version!	Thamnophis sirtalis elastin (ELN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELN ( XM_014065579.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013921054.1 Thamnophis sirtalis elastin (ELN), mRNA.

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CloneID	OTh23972
Clone ID Related Accession (Same CDS sequence)	XM_014065579.1
Accession Version	XM_014065579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	elastin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658800.1) and transcript sequence (GDKU01023374.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 26% of CDS bases assembly gap :: added 33 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTCCAA ATGTCCAGTG CGGGGTGTGC GTGTTTGTGT GTCACACAAT TGAAGCCTAC 
CCTTCAAGGG TTCCAGGTGC TGGAGCAGGA GTTCCAGGAG CAGGGTACTA CCCAGGAACA 
GCTGGCATCG GAGGCGTAGG GGGCCACGGC GGCACAGGAG CTGGCATTGG AGGTGGACTT 
GGAGGAACAG GTGGCCTATA TCCAGGAGGT GTCTATCCTG CTGGATCTGC CGCTGCAGCC 
GCTGCAGCCT ATAAAGCCGC CGCTAAAGCT GGACTTGGTG TACCTGGTGG TGGCTTAGGC 
GGACAACCTG GAGGTGGCAC AGGTGGACTA CCTGGAGGTG GCATAGGCGG ACTACCTGGA 
GGTGGAGGTG GAGGGCTACC TGGAGGTGGC TTTGGTGGAC TACCTGGAGG TGGAGGTGCA 
GGTCTACCTG GAGGTGGTGT GGGCGGATTA CCTGGTGCTG GTGGTGGAGT CAAGCCTGGG 
AAAATCCCTG GTGTTGGAAT TCCAGGAGTC TTCCCTGGAG GCGTACTTCC TGGCACAGGA 
TACCGCTATC CAGGTCTAGG AGTCTTGCCA GGAGTGGCAT CTGGAACAGG TGTCAAACAG 
AAAGTCCCAG GAGCTGGAGG TGGAGCCTTT GCTGGAATCC CTGGACTTGG AGGAATTGGA 
GGTGGACAGC AACCAGGCCT ACCTCTGGGT TATCCCATCA AGGCACCGAA ACTCCCAGGT 
AGTTATTATG GAAATCCTTA TGGTGCTGGC AAGCTGACTT TCGGCGGGGC AGGTGGTGCC 
TATGCTGGAG GACTTGGAGG AAAACCTGGC TATCCAACGG GTACAGGTGT AGGAGCTCAG 
GCCGCTGCTG CTAAGGCAGC CGCAAAATAT GCTGCTGGAC TTTTGCCTGG AGCAGGCGCT 
GGACTTTTGC CTGGAGCCGG GGGTGCCCTT GGCGGAGGAG GGCTGTTCCC TGGTGTTGGT 
GGTGCTGGAG GAGGACTAGG TGCCGGCGGA GCAGGAGTAG CGGCAGCAGT AGCTGCCAAA 
GTAGCAGCCA AAGCAGCAGC TTACGGTGGC AGAGGTGTCC CTGGAACTGG AGTGGGTGGT 
GGCGGCTATG GTCTTCCTGG AACTGGAGTT GGTGGTGTTG GTCTTCCTGG TGTTGGTGGA 
CTTCCGGGAG TTGGAGGAGG CGGACTAGGA GGAGTTGGTG TGGGAGGAAA TATGTACATG 
AATGGAATCC TATATGTGGT GGGATTGCAG AATGAGCATG GAGAGGGCGG GTTCCAGGTG 
GCATTGGCCC TGGTGGCGTC GGCCCTGGTG GCGTTGGCCC TGGTGGCGTC ATCATCCCTG 
GTGGCGTCGG CCCTGGTGGC GTCGGCCCTG GTGGCGTCGG CCCTGGTGGC GTCATCCCTG 
GTGGCGTCGG CCCTGGTGGC GTCGGCCCTG GTGGCGTCGG CCCTGGTGGC GTTGGCCCTG 
GTGGCGTTTT CCCAGGAGCC GGGACAGGAG GCAAGTCAGC AGCAGCTCTG GCAGCTAAGG 
CAGCAGCTAA AGCAGCAGCA TATGTTCCTG GCGCTGGTGG TGGACTTGTG CCTGGCTTTG 
GCGGAATCCC AGGAGGTGGA GTTCCTGGGG CAGCTGCAGC AGCGAAAGCA GCTGCTAAAG 
CAGCCAAATA CGGTCTCCGT CCCGGAGGTG GCGTCCTTCC TGGAGGTGGC GTCCTTCCTG 
GAGGTGGCGT CCTGCCTGGA GGTGGCGTCG TGCCTGGAGG TGGCGTCGTG CCTGGAGGTG 
GCGTCGTGCC TGGAGGTGGC GTCGTACCTG GAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013921054.1
CDS	1..1779
Translation	MSPNVQCGVCVFVCHTIEAYPSRVPGAGAGVPGAGYYPGTAGIGGVGGHGGTGAGIGGGLGGTGGLYPGGVYPAGSAAAAAAAYKAAAKAGLGVPGGGLGGQPGGGTGGLPGGGIGGLPGGGGGGLPGGGFGGLPGGGGAGLPGGGVGGLPGAGGGVKPGKIPGVGIPGVFPGGVLPGTGYRYPGLGVLPGVASGTGVKQKVPGAGGGAFAGIPGLGGIGGGQQPGLPLGYPIKAPKLPGSYYGNPYGAGKLTFGGAGGAYAGGLGGKPGYPTGTGVGAQAAAAKAAAKYAAGLLPGAGAGLLPGAGGALGGGGLFPGVGGAGGGLGAGGAGVAAAVAAKVAAKAAAYGGRGVPGTGVGGGGYGLPGTGVGGVGLPGVGGLPGVGGGGLGGVGVGGNMYMNGILYVVGLQNEHGEGGFQVALALVASALVALALVASSSLVASALVASALVASALVASSLVASALVASALVASALVALALVAFSQEPGQEASQQQLWQLRQQLKQQHMFLALVVDLCLALAESQEVEFLGQLQQRKQLLKQPNTVSVPEVASFLEVASFLEVASCLEVASCLEVASCLEVASCLEVASYLEE

Target ORF information:

RefSeq Version	XM_014065579.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis elastin (ELN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014065579.1

ORF Insert Sequence:

ATGTCTCCAA ATGTCCAGTG CGGGGTGTGC GTGTTTGTGT GTCACACAAT TGAAGCCTAC 
CCTTCAAGGG TTCCAGGTGC TGGAGCAGGA GTTCCAGGAG CAGGGTACTA CCCAGGAACA 
GCTGGCATCG GAGGCGTAGG GGGCCACGGC GGCACAGGAG CTGGCATTGG AGGTGGACTT 
GGAGGAACAG GTGGCCTATA TCCAGGAGGT GTCTATCCTG CTGGATCTGC CGCTGCAGCC 
GCTGCAGCCT ATAAAGCCGC CGCTAAAGCT GGACTTGGTG TACCTGGTGG TGGCTTAGGC 
GGACAACCTG GAGGTGGCAC AGGTGGACTA CCTGGAGGTG GCATAGGCGG ACTACCTGGA 
GGTGGAGGTG GAGGGCTACC TGGAGGTGGC TTTGGTGGAC TACCTGGAGG TGGAGGTGCA 
GGTCTACCTG GAGGTGGTGT GGGCGGATTA CCTGGTGCTG GTGGTGGAGT CAAGCCTGGG 
AAAATCCCTG GTGTTGGAAT TCCAGGAGTC TTCCCTGGAG GCGTACTTCC TGGCACAGGA 
TACCGCTATC CAGGTCTAGG AGTCTTGCCA GGAGTGGCAT CTGGAACAGG TGTCAAACAG 
AAAGTCCCAG GAGCTGGAGG TGGAGCCTTT GCTGGAATCC CTGGACTTGG AGGAATTGGA 
GGTGGACAGC AACCAGGCCT ACCTCTGGGT TATCCCATCA AGGCACCGAA ACTCCCAGGT 
AGTTATTATG GAAATCCTTA TGGTGCTGGC AAGCTGACTT TCGGCGGGGC AGGTGGTGCC 
TATGCTGGAG GACTTGGAGG AAAACCTGGC TATCCAACGG GTACAGGTGT AGGAGCTCAG 
GCCGCTGCTG CTAAGGCAGC CGCAAAATAT GCTGCTGGAC TTTTGCCTGG AGCAGGCGCT 
GGACTTTTGC CTGGAGCCGG GGGTGCCCTT GGCGGAGGAG GGCTGTTCCC TGGTGTTGGT 
GGTGCTGGAG GAGGACTAGG TGCCGGCGGA GCAGGAGTAG CGGCAGCAGT AGCTGCCAAA 
GTAGCAGCCA AAGCAGCAGC TTACGGTGGC AGAGGTGTCC CTGGAACTGG AGTGGGTGGT 
GGCGGCTATG GTCTTCCTGG AACTGGAGTT GGTGGTGTTG GTCTTCCTGG TGTTGGTGGA 
CTTCCGGGAG TTGGAGGAGG CGGACTAGGA GGAGTTGGTG TGGGAGGAAA TATGTACATG 
AATGGAATCC TATATGTGGT GGGATTGCAG AATGAGCATG GAGAGGGCGG GTTCCAGGTG 
GCATTGGCCC TGGTGGCGTC GGCCCTGGTG GCGTTGGCCC TGGTGGCGTC ATCATCCCTG 
GTGGCGTCGG CCCTGGTGGC GTCGGCCCTG GTGGCGTCGG CCCTGGTGGC GTCATCCCTG 
GTGGCGTCGG CCCTGGTGGC GTCGGCCCTG GTGGCGTCGG CCCTGGTGGC GTTGGCCCTG 
GTGGCGTTTT CCCAGGAGCC GGGACAGGAG GCAAGTCAGC AGCAGCTCTG GCAGCTAAGG 
CAGCAGCTAA AGCAGCAGCA TATGTTCCTG GCGCTGGTGG TGGACTTGTG CCTGGCTTTG 
GCGGAATCCC AGGAGGTGGA GTTCCTGGGG CAGCTGCAGC AGCGAAAGCA GCTGCTAAAG 
CAGCCAAATA CGGTCTCCGT CCCGGAGGTG GCGTCCTTCC TGGAGGTGGC GTCCTTCCTG 
GAGGTGGCGT CCTGCCTGGA GGTGGCGTCG TGCCTGGAGG TGGCGTCGTG CCTGGAGGTG 
GCGTCGTGCC TGGAGGTGGC GTCGTACCTG GAGGAGTAA