BRCA1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	BRCA1
Entrez Gene ID	106542500
Full Name	BRCA1, DNA repair associated
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014058254.1	XP_013913729.1	breast cancer type 1 susceptibility protein

Pathways from BioSystems
Gene Ontology

The following BRCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh18411	XM_014058254.1 Latest version!	Thamnophis sirtalis breast cancer 1, early onset (BRCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRCA1 ( XM_014058254.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913729.1 Thamnophis sirtalis breast cancer 1, early onset (BRCA1), mRNA.

Price & Availability↑

CloneID	OTh18411
Clone ID Related Accession (Same CDS sequence)	XM_014058254.1
Accession Version	XM_014058254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	breast cancer type 1 susceptibility protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658127.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CTGTTCCAAG TATTGGTGAC GTTCATGGAC TGCTGCTAGC TTTGCAGAAA 
CATTTGGAGT GTCCAATCTG TCTGGAGGTG ATGAAAGAGC CAGCGTCAAC AAACTGTGGT 
CATACCTATT GCAGGTTCTG TGTTTTGAAA CTGCTTACAC AGAAGAAAGA TGTAACACGG 
TGTCCACTTT GCAATGCCAA AATCACTAAA AGAGGTCTGA GGGATGATAT CTTTCTTAGA 
GAAGTAATCA AAGGAATTCT AGAATCCATC CATGCTTTTG AGTGCGATAC TGGATTCAAG 
TTTTCAGATG ATTTGTGTTA CCCCAAGAAA GTGATAGAAA CTGTTTCTGC TTCATCCACA 
TCTAAAGAAC AATTGATTAT TAACAGTAAG GGATATAGAA ATAGATTGAA ATCTATGAAG 
AAAGAAGAAG AGAGAAATAC TGAATTGGGT GACAACTGCA TCTTGCCACA GTGCAATGGA 
AATGAAACAA GATATTCTCT CAGAAAGAAA AAAAACTCTA GTAAAACTGT GGTTTTGGAA 
ATCAATGAGG AGCACAGATC AGTTTCATCT GAAGATACTT TTAAAGAAGA GAACATAGTC 
AGCTGCATGG ATTTTGAAAG TCCTTCCTCA TCTCAAGTTG GCAACGACTG TACTATGAAT 
CGAAGCCAAA ACTCTTGCCA CATAGAGCAT CCTGAAACAG GTGCCAAACA TGAACCATCC 
TTAAATATAG GTGAAGAAAT GATGCCCTAT GACAGTGAAG CAACCGCTCT GGAAGATTCA 
TTTGGTCAAT TCTCTCAGAG TGAAATTCTT ACCACACAGC AAAGGGATGC TATGCAAAAT 
AACTTAAAGC AACTGCAGCA GAAGATGGCT TTCATTGAAG CTGTCCTCAA AGAGGGAAGC 
CAGAGTATGA GCTCTGAAGG ATGGTCATTG GAAAGAGAGC AGACAGAAAC AAGAAAGGAA 
ATGCATAGTA TATTGGAAAA ATCAGTCTCT CCCTTGGCAC ATACCACCTG CTGTAGTACA 
AATAAAAGGA AAAGAAAGAG TCTACTTCTG ACTGGAAGGC AAAAAATGTC TCTGGTGGCA 
TCTGGCTTAA ACCAGAGTGA ATTGAAACTA GTTCGTAAAT TTGCCAAGAA AACTCAGAGC 
ACTTGGTGTA ATAAAATAAC TGAACAAACA ACTCATCTAA TAATGAAAAC AGATGAGGAT 
CTGGTCTGTG AAAGAACCCT GAAATACTTT ATAGGTGTTG CTGCACAAAA ATGGGTATTG 
AGCTACCAGT GGATTATACA TTCATTAGAA GCAGGAAGAG TTCATAAAGA GGAAGATTTT 
GAAGTGAGAG GAGATGTAAT CAATGGGAGA AACCACCAAG GACCTAAAAG AGCAAGAGAA 
TCACCTGTTG GAAAGCTTTT CCAAGGATTG GAGATTTGCT GCTATGGGCC ATTCACTGAC 
ATGCTCCCAG AACATCTGGA ATGGATTGTA CAACTTGGTG GTGCCTCCCT TGTGAAGCAA 
CCTCACTTAT TTACACATGC AACAGACTCC ATTGCTGTGA TAGTTGTTCA GCCTGATGCA 
TGGACCGAAG AAACCACCTG CCAAGCGCTC CCGCTGCAAT GCAGCACTCC TGTAGTTTCA 
CGGGAGTGGG TGCTGGACAG TGTTGCCTGC TATCAATGCC AGCCGTTCAG CGACTACATC 
ATTTCACAGG AACCAACTTC CATGTCTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913729.1
CDS	98..1810
Translation	MDLPVPSIGDVHGLLLALQKHLECPICLEVMKEPASTNCGHTYCRFCVLKLLTQKKDVTRCPLCNAKITKRGLRDDIFLREVIKGILESIHAFECDTGFKFSDDLCYPKKVIETVSASSTSKEQLIINSKGYRNRLKSMKKEEERNTELGDNCILPQCNGNETRYSLRKKKNSSKTVVLEINEEHRSVSSEDTFKEENIVSCMDFESPSSSQVGNDCTMNRSQNSCHIEHPETGAKHEPSLNIGEEMMPYDSEATALEDSFGQFSQSEILTTQQRDAMQNNLKQLQQKMAFIEAVLKEGSQSMSSEGWSLEREQTETRKEMHSILEKSVSPLAHTTCCSTNKRKRKSLLLTGRQKMSLVASGLNQSELKLVRKFAKKTQSTWCNKITEQTTHLIMKTDEDLVCERTLKYFIGVAAQKWVLSYQWIIHSLEAGRVHKEEDFEVRGDVINGRNHQGPKRARESPVGKLFQGLEICCYGPFTDMLPEHLEWIVQLGGASLVKQPHLFTHATDSIAVIVVQPDAWTEETTCQALPLQCSTPVVSREWVLDSVACYQCQPFSDYIISQEPTSMSE

Target ORF information:

RefSeq Version	XM_014058254.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis breast cancer 1, early onset (BRCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058254.1

ORF Insert Sequence:

ATGGATTTGC CTGTTCCAAG TATTGGTGAC GTTCATGGAC TGCTGCTAGC TTTGCAGAAA 
CATTTGGAGT GTCCAATCTG TCTGGAGGTG ATGAAAGAGC CAGCGTCAAC AAACTGTGGT 
CATACCTATT GCAGGTTCTG TGTTTTGAAA CTGCTTACAC AGAAGAAAGA TGTAACACGG 
TGTCCACTTT GCAATGCCAA AATCACTAAA AGAGGTCTGA GGGATGATAT CTTTCTTAGA 
GAAGTAATCA AAGGAATTCT AGAATCCATC CATGCTTTTG AGTGCGATAC TGGATTCAAG 
TTTTCAGATG ATTTGTGTTA CCCCAAGAAA GTGATAGAAA CTGTTTCTGC TTCATCCACA 
TCTAAAGAAC AATTGATTAT TAACAGTAAG GGATATAGAA ATAGATTGAA ATCTATGAAG 
AAAGAAGAAG AGAGAAATAC TGAATTGGGT GACAACTGCA TCTTGCCACA GTGCAATGGA 
AATGAAACAA GATATTCTCT CAGAAAGAAA AAAAACTCTA GTAAAACTGT GGTTTTGGAA 
ATCAATGAGG AGCACAGATC AGTTTCATCT GAAGATACTT TTAAAGAAGA GAACATAGTC 
AGCTGCATGG ATTTTGAAAG TCCTTCCTCA TCTCAAGTTG GCAACGACTG TACTATGAAT 
CGAAGCCAAA ACTCTTGCCA CATAGAGCAT CCTGAAACAG GTGCCAAACA TGAACCATCC 
TTAAATATAG GTGAAGAAAT GATGCCCTAT GACAGTGAAG CAACCGCTCT GGAAGATTCA 
TTTGGTCAAT TCTCTCAGAG TGAAATTCTT ACCACACAGC AAAGGGATGC TATGCAAAAT 
AACTTAAAGC AACTGCAGCA GAAGATGGCT TTCATTGAAG CTGTCCTCAA AGAGGGAAGC 
CAGAGTATGA GCTCTGAAGG ATGGTCATTG GAAAGAGAGC AGACAGAAAC AAGAAAGGAA 
ATGCATAGTA TATTGGAAAA ATCAGTCTCT CCCTTGGCAC ATACCACCTG CTGTAGTACA 
AATAAAAGGA AAAGAAAGAG TCTACTTCTG ACTGGAAGGC AAAAAATGTC TCTGGTGGCA 
TCTGGCTTAA ACCAGAGTGA ATTGAAACTA GTTCGTAAAT TTGCCAAGAA AACTCAGAGC 
ACTTGGTGTA ATAAAATAAC TGAACAAACA ACTCATCTAA TAATGAAAAC AGATGAGGAT 
CTGGTCTGTG AAAGAACCCT GAAATACTTT ATAGGTGTTG CTGCACAAAA ATGGGTATTG 
AGCTACCAGT GGATTATACA TTCATTAGAA GCAGGAAGAG TTCATAAAGA GGAAGATTTT 
GAAGTGAGAG GAGATGTAAT CAATGGGAGA AACCACCAAG GACCTAAAAG AGCAAGAGAA 
TCACCTGTTG GAAAGCTTTT CCAAGGATTG GAGATTTGCT GCTATGGGCC ATTCACTGAC 
ATGCTCCCAG AACATCTGGA ATGGATTGTA CAACTTGGTG GTGCCTCCCT TGTGAAGCAA 
CCTCACTTAT TTACACATGC AACAGACTCC ATTGCTGTGA TAGTTGTTCA GCCTGATGCA 
TGGACCGAAG AAACCACCTG CCAAGCGCTC CCGCTGCAAT GCAGCACTCC TGTAGTTTCA 
CGGGAGTGGG TGCTGGACAG TGTTGCCTGC TATCAATGCC AGCCGTTCAG CGACTACATC 
ATTTCACAGG AACCAACTTC CATGTCTGAA TAA