PIWIL2 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	PIWIL2
Entrez Gene ID	106539734
Full Name	piwi like RNA-mediated gene silencing 2
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014054595.1	XP_013910070.1	piwi-like protein 2

Pathways from BioSystems
Gene Ontology

The following PIWIL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh15683	XM_014054595.1 Latest version!	Thamnophis sirtalis piwi-like RNA-mediated gene silencing 2 (PIWIL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIWIL2 ( XM_014054595.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013910070.1 Thamnophis sirtalis piwi-like RNA-mediated gene silencing 2 (PIWIL2), mRNA.

Price & Availability↑

CloneID	OTh15683
Clone ID Related Accession (Same CDS sequence)	XM_014054595.1
Accession Version	XM_014054595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	piwi-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657922.1) and transcript sequence (GDKU01123648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTTCAGA TGTCTTTGCC TGGGCAAAGG GCAGAATCTC AGCCACAGGC GGGTGAGGCT 
AGAGCGATTG CAGAAATTTG GGGCAAAACC TTTCCTTCAA CAGTCAAGGC AGAAAGAGGA 
TACCCATCAC CAAGAGGTGC CCCAAAGGAG CCTTTCGTGA CTGTTCCAAC ATTGTCGTCC 
ATGGTTCAGG GCCTTAGTCT TGAGTCAGCT CCTAGGCCTC CTTCAGCAGT TTGTTCAGGC 
CGAGGCATTG CAGGGAGAGG AATGGGTGAC ATGAGCATGA AGCCCAATAT AGATGTCCTG 
GGACCGCAGA TGTTTGCAGG CTTGAGAGCA GGACTAGCTG GAGACAGCAA GGTGGAACAG 
GTGTTTTCTG GCCGTGGCAA GTATGTGGAA AGTGAAAAGC CATTTTTGGG GCTTGACAAA 
AGAAACATCT TGGGGCGTGG CCGAGCCTAC ACAAGCCCCT TCACTTCGCC TGTGGAAGGT 
CTTCCAGCAC CATCAATCAA ATCTAGTCCT GCTGTAATTT CGGATGAGCT TGTGGATAAA 
AAAGAACCGC TTAAGAAGCA AGGGTCAAAA GGAGTAGCAA TTCCTTTGGC ATTGAATCTG 
ATTCAAATAC ACTGTAAAAA TGAAGCTGTG TATCAGTACC ATGTGACATT CAGCCCAAAC 
ATGGAATGTA AAAGCGTGCG TCTTAGGATG CTGAAGGAGC ATCAGTCGGT GATAGGAGAC 
ATTTCCGCAT TTGATGGCTT TCTTCTCTAT TTGCCCATCA AGTTGCCTCA AAATATTAAC 
CTGAAGTGTG TAAGAAAGAC AGATGGAATG GAAGTCAATT TAAAGATACA GATGACCAAA 
ATTCTGGAAC CAAGTTCAGA GTTGTGTATC CCGTTTTACA ACGTTATTTT CAGAAAAGTG 
ATGAAGATTT TAGATATGAA ACTTGTTGGG AGAAATTTCT ATGATCCTAC CAGTGCTACT 
GTTCTTCAGC AGTACAGTTT GCAAATATGG CCTGGCTATG CAACCAGTAT CCGAAAGACA 
GATGGTGGCC TGTTTTTGTT GGTTGATGCT GTTCATAAGG TTATTCGGAA TGATTCAGTC 
CTGAATTTGA TGCATAGAAT TTATCAACAG AGTCGGGAAA ATTTCCAAGA CGAATGTACA 
AAGCAACTTA TTGGCAACAT CATAATTACC CGTTACAATA ATAAAAATTA CCGAATTGAT 
GACATTGATT GGAACAAGAC ACCACAGAAC AGTTTCACCA TGTCAGATGG GAAGGAAATC 
ACTTTTCTAG AGTATTACAG TAAAAATTAT GGGATCACCA TTAGAGAACT TGACCAGCCA 
CTGTTGATTT ACCGAGTTGA AAAAAGAAAA AATCCTCCAG AAAAGCTTCA GGAAGGTGAA 
ATCCTGTTAG TGCCAGAACT TTCCTTCTTG ACAGGCATTC CTGAGAAGAT GAGAAAAGAT 
TTTAGAATTA TGAAGGACCT TACGCAGAAA GTCAACATGA GTCCTGAACA GCATCATGCC 
TCTTTGCTCC AACTCCTGAG CAGAATTGAG AAGAATGAGA ATGCCCATAA GGAATTGTCC 
CGGTGGGGGC TTTTCTTGCA TGGAGATGTG TATAGGACCA CAGGACGCGT CCTTCCCATG 
GAAAGAATAA ATTTGAAGAA GTCCTCCTTC AATACCTCGG AAGACCTAAA CTGGACTAAA 
GAAATCAGCA GAGACTCCTG CATTTCTGCA GTTCCAATTC ATTTCTGGGC ACTATTTTAC 
CCAAAGCGGG CAATGGAACA AGCGCACGAA CTAGTCAGCA TACTGCAAAA AATCTCAGGA 
GCACTTGAAA TCTCATTAGC GGCACCTATT TGGAAAGAAT TGAAAGATGA CCGAATAGAG 
ACATATGCCA GAGCTATCAA ATCATTGCTA AGTAGCGAGG GATCAATACA ATTAGTCGTT 
TGCATTATCA CAGGAAATAA AGATGATTTG TACAGGGCCA TTAAAAGGCT GTGCAACGTA 
CATAATCCGG TTCCTTCCCA GGTTATTAAT GTTCGAACCA TTACAACACA GTATACTAAA 
CTTAGAAGTA TAGCCCAGAA GATTCTGTTG CAAATTAATT GCAAGTTAGG TGGAGAGCTG 
TGGGGTGTGG ACATTCCATT GAAACAGCTG ATGGTAATTG GCATAGACGT GTATCATGAT 
CCCAGCAGGG GGAAGCGCTC AGTTGTGGGC TTTGTGGCAA GTATCAATCC AATTGTGACA 
CAATGGTATT CCAGGGTTTT ATTCCAGACG CCTCATCAAG AAATAATAGA CAGCCTAAAA 
GTCTGTTTAG TAGCTGCTTT GCAGAAATTC TATGAGGTCA ACCATCATCT CCCGGAGAAG 
ATCGTGATTT ATAGAGATGG AGTTTCAGAT GGCCAGCTGA AAATAGTAGA AAGCTATGAA 
ATTCCACAGC TGGAGAAATG CTTTGAAGCT TTTAATAATT ACAACCCTAA AATGGTGGTG 
TTTGTGGTTC AGAAACAAAT CAGCACCAAC ATCTATTCAT CAGTTGGTCA ACAACTTTCA 
GCTCCTCCTC CAGGGACAGT ATTAGACCAT ACAGCCACCT CTCGAGACTG GGTGGACTTT 
TACTTGATAG CCCACTACTC GCCACAAGGT TGCGGCATTC CTACTCACTA CGTCTGTGTG 
AGGAATACAG CCAATCTGAC TCCTGATCAC ATGCAGAGGC TGACTTTCAA ACTTTGCCAC 
CTCTACTGGA ATTGGCCAGG AACTATCCGA GTGCCCGCCC CGTGCAAATA CGCGCATAAG 
CTGGCTTTTC TCTCAGGGCA CGTTCTCCAC CACGAACCAT CAATTGAGCT GTGTGACAAA 
CTCTTCTTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013910070.1
CDS	1..2835
Translation	MFQMSLPGQRAESQPQAGEARAIAEIWGKTFPSTVKAERGYPSPRGAPKEPFVTVPTLSSMVQGLSLESAPRPPSAVCSGRGIAGRGMGDMSMKPNIDVLGPQMFAGLRAGLAGDSKVEQVFSGRGKYVESEKPFLGLDKRNILGRGRAYTSPFTSPVEGLPAPSIKSSPAVISDELVDKKEPLKKQGSKGVAIPLALNLIQIHCKNEAVYQYHVTFSPNMECKSVRLRMLKEHQSVIGDISAFDGFLLYLPIKLPQNINLKCVRKTDGMEVNLKIQMTKILEPSSELCIPFYNVIFRKVMKILDMKLVGRNFYDPTSATVLQQYSLQIWPGYATSIRKTDGGLFLLVDAVHKVIRNDSVLNLMHRIYQQSRENFQDECTKQLIGNIIITRYNNKNYRIDDIDWNKTPQNSFTMSDGKEITFLEYYSKNYGITIRELDQPLLIYRVEKRKNPPEKLQEGEILLVPELSFLTGIPEKMRKDFRIMKDLTQKVNMSPEQHHASLLQLLSRIEKNENAHKELSRWGLFLHGDVYRTTGRVLPMERINLKKSSFNTSEDLNWTKEISRDSCISAVPIHFWALFYPKRAMEQAHELVSILQKISGALEISLAAPIWKELKDDRIETYARAIKSLLSSEGSIQLVVCIITGNKDDLYRAIKRLCNVHNPVPSQVINVRTITTQYTKLRSIAQKILLQINCKLGGELWGVDIPLKQLMVIGIDVYHDPSRGKRSVVGFVASINPIVTQWYSRVLFQTPHQEIIDSLKVCLVAALQKFYEVNHHLPEKIVIYRDGVSDGQLKIVESYEIPQLEKCFEAFNNYNPKMVVFVVQKQISTNIYSSVGQQLSAPPPGTVLDHTATSRDWVDFYLIAHYSPQGCGIPTHYVCVRNTANLTPDHMQRLTFKLCHLYWNWPGTIRVPAPCKYAHKLAFLSGHVLHHEPSIELCDKLFFL

Target ORF information:

RefSeq Version	XM_014054595.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis piwi-like RNA-mediated gene silencing 2 (PIWIL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014054595.1

ORF Insert Sequence:

ATGTTTCAGA TGTCTTTGCC TGGGCAAAGG GCAGAATCTC AGCCACAGGC GGGTGAGGCT 
AGAGCGATTG CAGAAATTTG GGGCAAAACC TTTCCTTCAA CAGTCAAGGC AGAAAGAGGA 
TACCCATCAC CAAGAGGTGC CCCAAAGGAG CCTTTCGTGA CTGTTCCAAC ATTGTCGTCC 
ATGGTTCAGG GCCTTAGTCT TGAGTCAGCT CCTAGGCCTC CTTCAGCAGT TTGTTCAGGC 
CGAGGCATTG CAGGGAGAGG AATGGGTGAC ATGAGCATGA AGCCCAATAT AGATGTCCTG 
GGACCGCAGA TGTTTGCAGG CTTGAGAGCA GGACTAGCTG GAGACAGCAA GGTGGAACAG 
GTGTTTTCTG GCCGTGGCAA GTATGTGGAA AGTGAAAAGC CATTTTTGGG GCTTGACAAA 
AGAAACATCT TGGGGCGTGG CCGAGCCTAC ACAAGCCCCT TCACTTCGCC TGTGGAAGGT 
CTTCCAGCAC CATCAATCAA ATCTAGTCCT GCTGTAATTT CGGATGAGCT TGTGGATAAA 
AAAGAACCGC TTAAGAAGCA AGGGTCAAAA GGAGTAGCAA TTCCTTTGGC ATTGAATCTG 
ATTCAAATAC ACTGTAAAAA TGAAGCTGTG TATCAGTACC ATGTGACATT CAGCCCAAAC 
ATGGAATGTA AAAGCGTGCG TCTTAGGATG CTGAAGGAGC ATCAGTCGGT GATAGGAGAC 
ATTTCCGCAT TTGATGGCTT TCTTCTCTAT TTGCCCATCA AGTTGCCTCA AAATATTAAC 
CTGAAGTGTG TAAGAAAGAC AGATGGAATG GAAGTCAATT TAAAGATACA GATGACCAAA 
ATTCTGGAAC CAAGTTCAGA GTTGTGTATC CCGTTTTACA ACGTTATTTT CAGAAAAGTG 
ATGAAGATTT TAGATATGAA ACTTGTTGGG AGAAATTTCT ATGATCCTAC CAGTGCTACT 
GTTCTTCAGC AGTACAGTTT GCAAATATGG CCTGGCTATG CAACCAGTAT CCGAAAGACA 
GATGGTGGCC TGTTTTTGTT GGTTGATGCT GTTCATAAGG TTATTCGGAA TGATTCAGTC 
CTGAATTTGA TGCATAGAAT TTATCAACAG AGTCGGGAAA ATTTCCAAGA CGAATGTACA 
AAGCAACTTA TTGGCAACAT CATAATTACC CGTTACAATA ATAAAAATTA CCGAATTGAT 
GACATTGATT GGAACAAGAC ACCACAGAAC AGTTTCACCA TGTCAGATGG GAAGGAAATC 
ACTTTTCTAG AGTATTACAG TAAAAATTAT GGGATCACCA TTAGAGAACT TGACCAGCCA 
CTGTTGATTT ACCGAGTTGA AAAAAGAAAA AATCCTCCAG AAAAGCTTCA GGAAGGTGAA 
ATCCTGTTAG TGCCAGAACT TTCCTTCTTG ACAGGCATTC CTGAGAAGAT GAGAAAAGAT 
TTTAGAATTA TGAAGGACCT TACGCAGAAA GTCAACATGA GTCCTGAACA GCATCATGCC 
TCTTTGCTCC AACTCCTGAG CAGAATTGAG AAGAATGAGA ATGCCCATAA GGAATTGTCC 
CGGTGGGGGC TTTTCTTGCA TGGAGATGTG TATAGGACCA CAGGACGCGT CCTTCCCATG 
GAAAGAATAA ATTTGAAGAA GTCCTCCTTC AATACCTCGG AAGACCTAAA CTGGACTAAA 
GAAATCAGCA GAGACTCCTG CATTTCTGCA GTTCCAATTC ATTTCTGGGC ACTATTTTAC 
CCAAAGCGGG CAATGGAACA AGCGCACGAA CTAGTCAGCA TACTGCAAAA AATCTCAGGA 
GCACTTGAAA TCTCATTAGC GGCACCTATT TGGAAAGAAT TGAAAGATGA CCGAATAGAG 
ACATATGCCA GAGCTATCAA ATCATTGCTA AGTAGCGAGG GATCAATACA ATTAGTCGTT 
TGCATTATCA CAGGAAATAA AGATGATTTG TACAGGGCCA TTAAAAGGCT GTGCAACGTA 
CATAATCCGG TTCCTTCCCA GGTTATTAAT GTTCGAACCA TTACAACACA GTATACTAAA 
CTTAGAAGTA TAGCCCAGAA GATTCTGTTG CAAATTAATT GCAAGTTAGG TGGAGAGCTG 
TGGGGTGTGG ACATTCCATT GAAACAGCTG ATGGTAATTG GCATAGACGT GTATCATGAT 
CCCAGCAGGG GGAAGCGCTC AGTTGTGGGC TTTGTGGCAA GTATCAATCC AATTGTGACA 
CAATGGTATT CCAGGGTTTT ATTCCAGACG CCTCATCAAG AAATAATAGA CAGCCTAAAA 
GTCTGTTTAG TAGCTGCTTT GCAGAAATTC TATGAGGTCA ACCATCATCT CCCGGAGAAG 
ATCGTGATTT ATAGAGATGG AGTTTCAGAT GGCCAGCTGA AAATAGTAGA AAGCTATGAA 
ATTCCACAGC TGGAGAAATG CTTTGAAGCT TTTAATAATT ACAACCCTAA AATGGTGGTG 
TTTGTGGTTC AGAAACAAAT CAGCACCAAC ATCTATTCAT CAGTTGGTCA ACAACTTTCA 
GCTCCTCCTC CAGGGACAGT ATTAGACCAT ACAGCCACCT CTCGAGACTG GGTGGACTTT 
TACTTGATAG CCCACTACTC GCCACAAGGT TGCGGCATTC CTACTCACTA CGTCTGTGTG 
AGGAATACAG CCAATCTGAC TCCTGATCAC ATGCAGAGGC TGACTTTCAA ACTTTGCCAC 
CTCTACTGGA ATTGGCCAGG AACTATCCGA GTGCCCGCCC CGTGCAAATA CGCGCATAAG 
CTGGCTTTTC TCTCAGGGCA CGTTCTCCAC CACGAACCAT CAATTGAGCT GTGTGACAAA 
CTCTTCTTCC TGTAG