LOC106538534 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106538534
Entrez Gene ID	106538534
Full Name	E3 ubiquitin-protein ligase RNF216-like
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014053039.1	XP_013908514.1	E3 ubiquitin-protein ligase RNF216-like

Pathways from BioSystems
Gene Ontology

The following LOC106538534 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106538534 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh14497	XM_014053039.1 Latest version!	Thamnophis sirtalis E3 ubiquitin-protein ligase RNF216-like (LOC106538534), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106538534 ( XM_014053039.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013908514.1 Thamnophis sirtalis E3 ubiquitin-protein ligase RNF216-like (LOC106538534), mRNA.

Price & Availability↑

CloneID	OTh14497
Clone ID Related Accession (Same CDS sequence)	XM_014053039.1
Accession Version	XM_014053039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	E3 ubiquitin-protein ligase RNF216-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657850.1) and transcript sequence (GDKU01092928.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 190 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCAGACG GAGGTCAGAA AGAAGTAATC GACTTGAATA GTTTCCGGAG CCATCGTGGA 
AAAGATAGGG GAGACGAGGG GCTCATCACT ATATCGGACT CCTCGGACGA AGACCTGCCC 
GTGATGTTGG ACACACCCGT CCGCCAGCAG TGGGAAGACG AGGATGATGA AGACGTGGTC 
ATCTTGACGG AGACAGCCAC CAAATCATTC CTGCGGCCCA ACGTCATCAA GCCCGCCGCC 
CCCTGGCAAG TCGTTGACAA GACGGGGAAA GGAGGATCAG ATGGCGACGT GGTGGCGCTT 
CCTCACCAAG ACCACAAGGC CAAAGCCCCA ACTTCTCCGA GGCTGCCCGG CACCCGCCGG 
AACTGCCTGA AGGCGGGAAG CGCCGCGGGG CGGGGCAGGC CGAGCCAGGC CGAAGTCTTG 
GAGTTACCCG ATTCTCCGGA TCCCGCCGAC CCCGGGGAGG TTAGCGGGAC AGAGGCTGGG 
CCCAGCGTGG TTCCGAAGAG AGAGGCGGCT CCGGAGGTTA TTAATTTGGA GGAAGTGGGG 
CCCGATCGGG AGAGCCAAGA AGAAGAAGAA GAAGAAGAGG GAGATCGGAC GCTTCCACCA 
CAGCAGGACC CCAAACTTTT CCATTGCGCG AAGGAGAACA TGCGGCTGGA TCATCCGTAC 
TTCCAGCCGG CGAAGGAGGA GGCACGGACT GGGGTCAATC CTCTCCCTTC TCAGCCTGGA 
CCGTTGGAAA GAGAACTTGG GCCTTTGCCG AGGGTGTACC AGGGAGAGAT TCCCGGGCCG 
GCTTTCCCCA GGCCCGAGCC TCAGCAGGAT GGGATCCCAG GCCCCGCTTC CCCTCAACTA 
GCTCACCCCC CAGAGGAAAA CGGAGGCCAG GAGGCGGTAA TAAACGAGCA GGCCGGGCCG 
GCGTTTCCAG TCCAAGGGTC GCTGAATACC AGCTCCGGCG AGCTTCCCAA GCAGACGGCT 
ACTCTGCTGG ACAAAGACCT CCTTGTTTTA CTCACCCGAG AAACGGAAGC CAGGTTCCCA 
GATGCAACGA GAGAGTACAT CGAAGAGCTG ATCCAGAGCA AAAACTGCAA CGACTTAAAC 
ATACTCTGTA ATTTTCTTCT GGAAAATCCA GACTATCCAA AGAAGGAACT CAATATGGTT 
TTAAATCCAA ATAGCAGCCT GCTTTCCAGC CAAGACGAGA CAAAGGTGCC TAAAGTGGAC 
TATTTTGACT TCTCCAAACT GGATCCGCTC GACCAACGTT GCTTCATTCA AGCCGCCGAC 
CTCCTCATGG CCGACTTCAA AATGCTGAGC AGCCAAGACA TCAAGTGGGC CCTCCACGAA 
CTCAAGGGAC ACTATGCAAT CACACGAAAG GTCCTCGCCT CTTGCTTCTC CTTCCTTCCT 
TCCTTGCTAG GAACCTCCCC TCTTTTAATG TCCGAAAGGG AGACGCGGAG GCCAGGCTCA 
AAAAAGAAAC CCCTTAGGTG CCCGGAATTC AAAGGCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013908514.1
CDS	1..1482
Translation	MADGGQKEVIDLNSFRSHRGKDRGDEGLITISDSSDEDLPVMLDTPVRQQWEDEDDEDVVILTETATKSFLRPNVIKPAAPWQVVDKTGKGGSDGDVVALPHQDHKAKAPTSPRLPGTRRNCLKAGSAAGRGRPSQAEVLELPDSPDPADPGEVSGTEAGPSVVPKREAAPEVINLEEVGPDRESQEEEEEEEGDRTLPPQQDPKLFHCAKENMRLDHPYFQPAKEEARTGVNPLPSQPGPLERELGPLPRVYQGEIPGPAFPRPEPQQDGIPGPASPQLAHPPEENGGQEAVINEQAGPAFPVQGSLNTSSGELPKQTATLLDKDLLVLLTRETEARFPDATREYIEELIQSKNCNDLNILCNFLLENPDYPKKELNMVLNPNSSLLSSQDETKVPKVDYFDFSKLDPLDQRCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKVLASCFSFLPSLLGTSPLLMSERETRRPGSKKKPLRCPEFKGL

Target ORF information:

RefSeq Version	XM_014053039.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis E3 ubiquitin-protein ligase RNF216-like (LOC106538534), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014053039.1

ORF Insert Sequence:

ATGGCAGACG GAGGTCAGAA AGAAGTAATC GACTTGAATA GTTTCCGGAG CCATCGTGGA 
AAAGATAGGG GAGACGAGGG GCTCATCACT ATATCGGACT CCTCGGACGA AGACCTGCCC 
GTGATGTTGG ACACACCCGT CCGCCAGCAG TGGGAAGACG AGGATGATGA AGACGTGGTC 
ATCTTGACGG AGACAGCCAC CAAATCATTC CTGCGGCCCA ACGTCATCAA GCCCGCCGCC 
CCCTGGCAAG TCGTTGACAA GACGGGGAAA GGAGGATCAG ATGGCGACGT GGTGGCGCTT 
CCTCACCAAG ACCACAAGGC CAAAGCCCCA ACTTCTCCGA GGCTGCCCGG CACCCGCCGG 
AACTGCCTGA AGGCGGGAAG CGCCGCGGGG CGGGGCAGGC CGAGCCAGGC CGAAGTCTTG 
GAGTTACCCG ATTCTCCGGA TCCCGCCGAC CCCGGGGAGG TTAGCGGGAC AGAGGCTGGG 
CCCAGCGTGG TTCCGAAGAG AGAGGCGGCT CCGGAGGTTA TTAATTTGGA GGAAGTGGGG 
CCCGATCGGG AGAGCCAAGA AGAAGAAGAA GAAGAAGAGG GAGATCGGAC GCTTCCACCA 
CAGCAGGACC CCAAACTTTT CCATTGCGCG AAGGAGAACA TGCGGCTGGA TCATCCGTAC 
TTCCAGCCGG CGAAGGAGGA GGCACGGACT GGGGTCAATC CTCTCCCTTC TCAGCCTGGA 
CCGTTGGAAA GAGAACTTGG GCCTTTGCCG AGGGTGTACC AGGGAGAGAT TCCCGGGCCG 
GCTTTCCCCA GGCCCGAGCC TCAGCAGGAT GGGATCCCAG GCCCCGCTTC CCCTCAACTA 
GCTCACCCCC CAGAGGAAAA CGGAGGCCAG GAGGCGGTAA TAAACGAGCA GGCCGGGCCG 
GCGTTTCCAG TCCAAGGGTC GCTGAATACC AGCTCCGGCG AGCTTCCCAA GCAGACGGCT 
ACTCTGCTGG ACAAAGACCT CCTTGTTTTA CTCACCCGAG AAACGGAAGC CAGGTTCCCA 
GATGCAACGA GAGAGTACAT CGAAGAGCTG ATCCAGAGCA AAAACTGCAA CGACTTAAAC 
ATACTCTGTA ATTTTCTTCT GGAAAATCCA GACTATCCAA AGAAGGAACT CAATATGGTT 
TTAAATCCAA ATAGCAGCCT GCTTTCCAGC CAAGACGAGA CAAAGGTGCC TAAAGTGGAC 
TATTTTGACT TCTCCAAACT GGATCCGCTC GACCAACGTT GCTTCATTCA AGCCGCCGAC 
CTCCTCATGG CCGACTTCAA AATGCTGAGC AGCCAAGACA TCAAGTGGGC CCTCCACGAA 
CTCAAGGGAC ACTATGCAAT CACACGAAAG GTCCTCGCCT CTTGCTTCTC CTTCCTTCCT 
TCCTTGCTAG GAACCTCCCC TCTTTTAATG TCCGAAAGGG AGACGCGGAG GCCAGGCTCA 
AAAAAGAAAC CCCTTAGGTG CCCGGAATTC AAAGGCCTCT GA