LOC107364833 cDNA ORF clone, Tetranychus urticae(two-spotted spider mite)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107364833
Entrez Gene ID	107364833
Full Name	ubiquitin carboxyl-terminal hydrolase 31-like
Gene Type	protein-coding
Organism	Tetranychus urticae(two-spotted spider mite)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015932272.2	XP_015787758.1	ubiquitin carboxyl-terminal hydrolase 31
XM_015932272.1	XP_015787758.1	ubiquitin carboxyl-terminal hydrolase 31-like

Pathways from BioSystems
Gene Ontology

The following LOC107364833 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107364833 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTe20950	XM_015932272.2 Latest version!	Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31 (LOC107364833), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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OTe20950	XM_015932272.1	Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31-like (LOC107364833), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107364833 ( XM_015932272.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015787758.1 Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31 (LOC107364833), mRNA.

Price & Availability↑

CloneID	OTe20950
Clone ID Related Accession (Same CDS sequence)	XM_015932272.2 , XM_015932272.1
Accession Version	XM_015932272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	ubiquitin carboxyl-terminal hydrolase 31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015932272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGAGA AAATTATCAA TCGTTTTGGC GAAGAGACCA CAGCAAGTAT TAAACAGGTC 
ATGGATGGTT CAGGCCTCGC CCAAGCTATT GGTCTACCAA CATCAACATC AACATCACCA 
GCTTCATCTA CTTCATCATC AACCGCAACT TCATCCCTTG GTAAATTGAT TGAGGTTAAT 
TTCTTAGATA AATCTCTTCA CCTTGACAGT GATAAAATTT ACCAATTTAA AGAGGTTTCA 
AAGTTAAATG GTTTGAAACA GTTTTCTGAC CAATACTCTA TTGAGCAACT TAAGATTGAT 
CTTAATAATA AAGATATAGA ATCTAGTTGT GACCTCTTGG TTAAAGATCA GCAACAACAT 
CATCATCAAC ATTTTCATCA TCATCTAAAC TCTCAACATC AAAAGCAACC ACAGCAATCA 
AACTCTTCAA ACCAACCAAA CCAGGAAAAT AATCCTCAAC CATCTCCTCA TCTACATCAT 
CAACCCATTA GTAATCCATC ATGTGTTACA ATTAGTGATG ATGACTTAAG AGAGGAAAGT 
AAACTTCCAG TCATCAATGG TACAGTAAAT GAGGATCATG ATAGTTCACT GAAATCAGCC 
TCACCCCAGT CAGAACCAGC TTCTATTCCC ATTTGTGTGG ATAAACTTGA AAAACCATGT 
ATTATTAATT CAAATGAAAA TGGCATTGAA AGGCCTAAGC TAAACCAAGA CGTTGCTCGA 
CAAGCTGTAA CACTACCAAG AACTCAAGGA CAAACCCACA AAGATACATC TACCTTACGT 
AGAAATGGTG AAAATGATAA AAAGCGTTCT AGAAGTTTTA TAGTTCGTTT ATTATCAAAT 
TTGCCATATC TTTCAATTTC CGGGTCTCGT TTAAACGGCA AAGACGATGA CGAGGATTCA 
TCTACCGACA GTTATAAAAG TCTACGAAGA TCTAGAGAAC GATCAAATAA TCATCAATCG 
ACAAATAAAT TATCTAATTC AATGTCAAGC CAAGATGCTG TAAATCTATT CACATGTAAT 
TGTTCAGAGG GAACTCATGG TAAAACAACT AATCAAACTA AATCGAAGCC GTTAACCTTG 
TCTGGAATTA AAAACCATGG CAATACTTGT TTCATGAATG CTATTCTACA ATGTTTATCT 
CATACCGATT TACTTATAGA ATATTTTAAT GATACCCAAT TTTATTATAA AGATTTAATT 
ACACGACAAC AGCTCAATCT GTGTAAAAAT GGCTCAAGTA GTGAAGTCAC CGCTCAATGT 
GCAACTTTAT TTCAATCTTT ATGGAAAAAC AATTATTCTC CTGATTTATC TAGCAAATTT 
AAATCTTTAA TTGGTAAACA ATGTAAGCAA TATGAGGATG GTGGTCAACA TGATGCACAA 
GAGTTTCTAT TTTTTTTGTT GGACATTTTC CATGAAGATC TCAACGTTGC CATCAAAAAG 
AAGGCCAAAA AGTTCAAGAT TCCCAGTACC TGGAACCGGC CTGATGAAGT TATAGCCGCG 
GAAACTTTAT CGATTTATAC AAGATGTGAC AATAGTTTTA TTCGCAAAGT TTTTCAAGCT 
CAATTACGAT CATCGGTTGT TTGTCGTTCA TGTAAAAGCC ATAGCAATAC ATTTGACCCG 
TATATGTGCC TTTCATTACC GGTTCCCACC AAGCTTACCC ACATCTGTAT CATTAACGTT 
TCATACCTTG ATAATGTTCC AAAAAAGATC AAAATGGCTG TTACCATCGA GACTCAAGCT 
ACGCTTAAAG AAATGAGACA TAAAATTTCA AGATTAATAA AAATACCTGA AAACCAATTG 
GTGCTGCTGA TAAATGACAG TTCAACTGGT ATCAAAGAAT TGAATCAATA TAAAACTCCA 
ATCAGTGAAT TTTTAAATGA AAATGATGAG ATAGAAGCTA TTGAAACTCC TAAATTAGAC 
ATGAATCAAA ACTGTAATTC AGGATCTCCC AAATCAACCT CAACAATATC CCAACAATTA 
ACCCTTGTAT GGACTAATCG TGTCGGAATT GGTAGTTCTG ATAAAAACAA ATTTTTTGGA 
CCTTATTTTA GTTGCACAAT CAATCGAGAG GCAAGTTTCA AAGATATACA GAGTGAATTA 
GTGAAAACAA TGCAACCGAT TTTGAGGTTA ACCTCTGTGT CAAGTTGTAT CAAAGATTTA 
CCCTTGTTTA AATTGAGAGT TGTTGGTGGC ATCCAAGATC GATGTTATTT ACCCGAGCAT 
GGTGATCATC CTTTATACTC TTCCACTGTC AACGAAGCAT TAGCTCATTG TAAAGAGAAA 
GATTATCGTG GACCAACCTA TGTGATGTTG ATTGTTGAAT GGGATTCTGA TTTAAAGCAA 
ACACTGGTCG TTGATGATGA TGAGGCATTA ATTGTTACCG AGGATCCAAA CATGGAATAT 
GTTATTGCTC GTAATCGTCT ATCTAACCAG GCAACTTTAG AAGATTGTTT CGACATGTAT 
TTCCGTGAAG AGAAACTTGG TGCTGATGAT GCTTGGATGT GTCCATCTTG TAAAAAGAAG 
CAGCAATGTA TTAAGAAACT TAGTCTCTGG TCAACCCCTG ATATACTTAT TATTCATCTA 
AAACGCTTCC GTCAAGCAAC TAATCTTCAA CGAACCAAAT TATCAACTTT AGTCAACTTT 
CCATTCACTG GTTTGGATAT GAATCCCTAC TTGAGTAAAC GCAAAGCTAA TCTAGACGAA 
TACAAAGAAT CTAATGGGGA TGTTAATAAT TTATCTTACA TTTTGTATCC TTGGAAAAAA 
CCGCGAGAAA AAGAGGTTCT TCCTTATTAT TCGGAAGATA ATATCTATGA TTTATATGCT 
GTCTGTAATC ACCATGGGAA CATGTCATCA GGACATTATA CAGCTTACTG TCGTGACATT 
AATGATCATA AATGGTATTT ATTTGATGAT CAACAAGTTA CTAAGGTTTC AGAAAACTCA 
TTAGTTACTG GAGATGCTTA CATCTTATTT TACCAACGAA GTACTATTCC ACCATTTACT 
CTTCCTCCTT CAGAATCACC GTCTATGAGA ACAAAACGAG CATCGTCGTC ATCATCAACA 
TCGTCATCGT CATCATCTTC ATCATCTTCC TCCTCTTCAT CTTCTTCATC ATTATCATCC 
AACGCGGATC AATCCTCAAC TAATTCATTG ACATCTCAAT CTAACGTCAG CTTAAATAAT 
GACAATCAAT CTTCACCGGT TAAATTAGCA AACTCAACTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015787758.1
CDS	156..3380
Translation	MVEKIINRFGEETTASIKQVMDGSGLAQAIGLPTSTSTSPASSTSSSTATSSLGKLIEVNFLDKSLHLDSDKIYQFKEVSKLNGLKQFSDQYSIEQLKIDLNNKDIESSCDLLVKDQQQHHHQHFHHHLNSQHQKQPQQSNSSNQPNQENNPQPSPHLHHQPISNPSCVTISDDDLREESKLPVINGTVNEDHDSSLKSASPQSEPASIPICVDKLEKPCIINSNENGIERPKLNQDVARQAVTLPRTQGQTHKDTSTLRRNGENDKKRSRSFIVRLLSNLPYLSISGSRLNGKDDDEDSSTDSYKSLRRSRERSNNHQSTNKLSNSMSSQDAVNLFTCNCSEGTHGKTTNQTKSKPLTLSGIKNHGNTCFMNAILQCLSHTDLLIEYFNDTQFYYKDLITRQQLNLCKNGSSSEVTAQCATLFQSLWKNNYSPDLSSKFKSLIGKQCKQYEDGGQHDAQEFLFFLLDIFHEDLNVAIKKKAKKFKIPSTWNRPDEVIAAETLSIYTRCDNSFIRKVFQAQLRSSVVCRSCKSHSNTFDPYMCLSLPVPTKLTHICIINVSYLDNVPKKIKMAVTIETQATLKEMRHKISRLIKIPENQLVLLINDSSTGIKELNQYKTPISEFLNENDEIEAIETPKLDMNQNCNSGSPKSTSTISQQLTLVWTNRVGIGSSDKNKFFGPYFSCTINREASFKDIQSELVKTMQPILRLTSVSSCIKDLPLFKLRVVGGIQDRCYLPEHGDHPLYSSTVNEALAHCKEKDYRGPTYVMLIVEWDSDLKQTLVVDDDEALIVTEDPNMEYVIARNRLSNQATLEDCFDMYFREEKLGADDAWMCPSCKKKQQCIKKLSLWSTPDILIIHLKRFRQATNLQRTKLSTLVNFPFTGLDMNPYLSKRKANLDEYKESNGDVNNLSYILYPWKKPREKEVLPYYSEDNIYDLYAVCNHHGNMSSGHYTAYCRDINDHKWYLFDDQQVTKVSENSLVTGDAYILFYQRSTIPPFTLPPSESPSMRTKRASSSSSTSSSSSSSSSSSSSSSSSLSSNADQSSTNSLTSQSNVSLNNDNQSSPVKLANSTL

Target ORF information:

RefSeq Version	XM_015932272.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31 (LOC107364833), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015932272.2

ORF Insert Sequence:

ATGGTCGAGA AAATTATCAA TCGTTTTGGC GAAGAGACCA CAGCAAGTAT TAAACAGGTC 
ATGGATGGTT CAGGCCTCGC CCAAGCTATT GGTCTACCAA CATCAACATC AACATCACCA 
GCTTCATCTA CTTCATCATC AACCGCAACT TCATCCCTTG GTAAATTGAT TGAGGTTAAT 
TTCTTAGATA AATCTCTTCA CCTTGACAGT GATAAAATTT ACCAATTTAA AGAGGTTTCA 
AAGTTAAATG GTTTGAAACA GTTTTCTGAC CAATACTCTA TTGAGCAACT TAAGATTGAT 
CTTAATAATA AAGATATAGA ATCTAGTTGT GACCTCTTGG TTAAAGATCA GCAACAACAT 
CATCATCAAC ATTTTCATCA TCATCTAAAC TCTCAACATC AAAAGCAACC ACAGCAATCA 
AACTCTTCAA ACCAACCAAA CCAGGAAAAT AATCCTCAAC CATCTCCTCA TCTACATCAT 
CAACCCATTA GTAATCCATC ATGTGTTACA ATTAGTGATG ATGACTTAAG AGAGGAAAGT 
AAACTTCCAG TCATCAATGG TACAGTAAAT GAGGATCATG ATAGTTCACT GAAATCAGCC 
TCACCCCAGT CAGAACCAGC TTCTATTCCC ATTTGTGTGG ATAAACTTGA AAAACCATGT 
ATTATTAATT CAAATGAAAA TGGCATTGAA AGGCCTAAGC TAAACCAAGA CGTTGCTCGA 
CAAGCTGTAA CACTACCAAG AACTCAAGGA CAAACCCACA AAGATACATC TACCTTACGT 
AGAAATGGTG AAAATGATAA AAAGCGTTCT AGAAGTTTTA TAGTTCGTTT ATTATCAAAT 
TTGCCATATC TTTCAATTTC CGGGTCTCGT TTAAACGGCA AAGACGATGA CGAGGATTCA 
TCTACCGACA GTTATAAAAG TCTACGAAGA TCTAGAGAAC GATCAAATAA TCATCAATCG 
ACAAATAAAT TATCTAATTC AATGTCAAGC CAAGATGCTG TAAATCTATT CACATGTAAT 
TGTTCAGAGG GAACTCATGG TAAAACAACT AATCAAACTA AATCGAAGCC GTTAACCTTG 
TCTGGAATTA AAAACCATGG CAATACTTGT TTCATGAATG CTATTCTACA ATGTTTATCT 
CATACCGATT TACTTATAGA ATATTTTAAT GATACCCAAT TTTATTATAA AGATTTAATT 
ACACGACAAC AGCTCAATCT GTGTAAAAAT GGCTCAAGTA GTGAAGTCAC CGCTCAATGT 
GCAACTTTAT TTCAATCTTT ATGGAAAAAC AATTATTCTC CTGATTTATC TAGCAAATTT 
AAATCTTTAA TTGGTAAACA ATGTAAGCAA TATGAGGATG GTGGTCAACA TGATGCACAA 
GAGTTTCTAT TTTTTTTGTT GGACATTTTC CATGAAGATC TCAACGTTGC CATCAAAAAG 
AAGGCCAAAA AGTTCAAGAT TCCCAGTACC TGGAACCGGC CTGATGAAGT TATAGCCGCG 
GAAACTTTAT CGATTTATAC AAGATGTGAC AATAGTTTTA TTCGCAAAGT TTTTCAAGCT 
CAATTACGAT CATCGGTTGT TTGTCGTTCA TGTAAAAGCC ATAGCAATAC ATTTGACCCG 
TATATGTGCC TTTCATTACC GGTTCCCACC AAGCTTACCC ACATCTGTAT CATTAACGTT 
TCATACCTTG ATAATGTTCC AAAAAAGATC AAAATGGCTG TTACCATCGA GACTCAAGCT 
ACGCTTAAAG AAATGAGACA TAAAATTTCA AGATTAATAA AAATACCTGA AAACCAATTG 
GTGCTGCTGA TAAATGACAG TTCAACTGGT ATCAAAGAAT TGAATCAATA TAAAACTCCA 
ATCAGTGAAT TTTTAAATGA AAATGATGAG ATAGAAGCTA TTGAAACTCC TAAATTAGAC 
ATGAATCAAA ACTGTAATTC AGGATCTCCC AAATCAACCT CAACAATATC CCAACAATTA 
ACCCTTGTAT GGACTAATCG TGTCGGAATT GGTAGTTCTG ATAAAAACAA ATTTTTTGGA 
CCTTATTTTA GTTGCACAAT CAATCGAGAG GCAAGTTTCA AAGATATACA GAGTGAATTA 
GTGAAAACAA TGCAACCGAT TTTGAGGTTA ACCTCTGTGT CAAGTTGTAT CAAAGATTTA 
CCCTTGTTTA AATTGAGAGT TGTTGGTGGC ATCCAAGATC GATGTTATTT ACCCGAGCAT 
GGTGATCATC CTTTATACTC TTCCACTGTC AACGAAGCAT TAGCTCATTG TAAAGAGAAA 
GATTATCGTG GACCAACCTA TGTGATGTTG ATTGTTGAAT GGGATTCTGA TTTAAAGCAA 
ACACTGGTCG TTGATGATGA TGAGGCATTA ATTGTTACCG AGGATCCAAA CATGGAATAT 
GTTATTGCTC GTAATCGTCT ATCTAACCAG GCAACTTTAG AAGATTGTTT CGACATGTAT 
TTCCGTGAAG AGAAACTTGG TGCTGATGAT GCTTGGATGT GTCCATCTTG TAAAAAGAAG 
CAGCAATGTA TTAAGAAACT TAGTCTCTGG TCAACCCCTG ATATACTTAT TATTCATCTA 
AAACGCTTCC GTCAAGCAAC TAATCTTCAA CGAACCAAAT TATCAACTTT AGTCAACTTT 
CCATTCACTG GTTTGGATAT GAATCCCTAC TTGAGTAAAC GCAAAGCTAA TCTAGACGAA 
TACAAAGAAT CTAATGGGGA TGTTAATAAT TTATCTTACA TTTTGTATCC TTGGAAAAAA 
CCGCGAGAAA AAGAGGTTCT TCCTTATTAT TCGGAAGATA ATATCTATGA TTTATATGCT 
GTCTGTAATC ACCATGGGAA CATGTCATCA GGACATTATA CAGCTTACTG TCGTGACATT 
AATGATCATA AATGGTATTT ATTTGATGAT CAACAAGTTA CTAAGGTTTC AGAAAACTCA 
TTAGTTACTG GAGATGCTTA CATCTTATTT TACCAACGAA GTACTATTCC ACCATTTACT 
CTTCCTCCTT CAGAATCACC GTCTATGAGA ACAAAACGAG CATCGTCGTC ATCATCAACA 
TCGTCATCGT CATCATCTTC ATCATCTTCC TCCTCTTCAT CTTCTTCATC ATTATCATCC 
AACGCGGATC AATCCTCAAC TAATTCATTG ACATCTCAAT CTAACGTCAG CTTAAATAAT 
GACAATCAAT CTTCACCGGT TAAATTAGCA AACTCAACTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364833 ( XM_015932272.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015787758.1 Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31-like (LOC107364833), mRNA.

Price & Availability↑

CloneID	OTe20950
Clone ID Related Accession (Same CDS sequence)	XM_015932272.2 , XM_015932272.1
Accession Version	XM_015932272.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	ubiquitin carboxyl-terminal hydrolase 31-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGAGA AAATTATCAA TCGTTTTGGC GAAGAGACCA CAGCAAGTAT TAAACAGGTC 
ATGGATGGTT CAGGCCTCGC CCAAGCTATT GGTCTACCAA CATCAACATC AACATCACCA 
GCTTCATCTA CTTCATCATC AACCGCAACT TCATCCCTTG GTAAATTGAT TGAGGTTAAT 
TTCTTAGATA AATCTCTTCA CCTTGACAGT GATAAAATTT ACCAATTTAA AGAGGTTTCA 
AAGTTAAATG GTTTGAAACA GTTTTCTGAC CAATACTCTA TTGAGCAACT TAAGATTGAT 
CTTAATAATA AAGATATAGA ATCTAGTTGT GACCTCTTGG TTAAAGATCA GCAACAACAT 
CATCATCAAC ATTTTCATCA TCATCTAAAC TCTCAACATC AAAAGCAACC ACAGCAATCA 
AACTCTTCAA ACCAACCAAA CCAGGAAAAT AATCCTCAAC CATCTCCTCA TCTACATCAT 
CAACCCATTA GTAATCCATC ATGTGTTACA ATTAGTGATG ATGACTTAAG AGAGGAAAGT 
AAACTTCCAG TCATCAATGG TACAGTAAAT GAGGATCATG ATAGTTCACT GAAATCAGCC 
TCACCCCAGT CAGAACCAGC TTCTATTCCC ATTTGTGTGG ATAAACTTGA AAAACCATGT 
ATTATTAATT CAAATGAAAA TGGCATTGAA AGGCCTAAGC TAAACCAAGA CGTTGCTCGA 
CAAGCTGTAA CACTACCAAG AACTCAAGGA CAAACCCACA AAGATACATC TACCTTACGT 
AGAAATGGTG AAAATGATAA AAAGCGTTCT AGAAGTTTTA TAGTTCGTTT ATTATCAAAT 
TTGCCATATC TTTCAATTTC CGGGTCTCGT TTAAACGGCA AAGACGATGA CGAGGATTCA 
TCTACCGACA GTTATAAAAG TCTACGAAGA TCTAGAGAAC GATCAAATAA TCATCAATCG 
ACAAATAAAT TATCTAATTC AATGTCAAGC CAAGATGCTG TAAATCTATT CACATGTAAT 
TGTTCAGAGG GAACTCATGG TAAAACAACT AATCAAACTA AATCGAAGCC GTTAACCTTG 
TCTGGAATTA AAAACCATGG CAATACTTGT TTCATGAATG CTATTCTACA ATGTTTATCT 
CATACCGATT TACTTATAGA ATATTTTAAT GATACCCAAT TTTATTATAA AGATTTAATT 
ACACGACAAC AGCTCAATCT GTGTAAAAAT GGCTCAAGTA GTGAAGTCAC CGCTCAATGT 
GCAACTTTAT TTCAATCTTT ATGGAAAAAC AATTATTCTC CTGATTTATC TAGCAAATTT 
AAATCTTTAA TTGGTAAACA ATGTAAGCAA TATGAGGATG GTGGTCAACA TGATGCACAA 
GAGTTTCTAT TTTTTTTGTT GGACATTTTC CATGAAGATC TCAACGTTGC CATCAAAAAG 
AAGGCCAAAA AGTTCAAGAT TCCCAGTACC TGGAACCGGC CTGATGAAGT TATAGCCGCG 
GAAACTTTAT CGATTTATAC AAGATGTGAC AATAGTTTTA TTCGCAAAGT TTTTCAAGCT 
CAATTACGAT CATCGGTTGT TTGTCGTTCA TGTAAAAGCC ATAGCAATAC ATTTGACCCG 
TATATGTGCC TTTCATTACC GGTTCCCACC AAGCTTACCC ACATCTGTAT CATTAACGTT 
TCATACCTTG ATAATGTTCC AAAAAAGATC AAAATGGCTG TTACCATCGA GACTCAAGCT 
ACGCTTAAAG AAATGAGACA TAAAATTTCA AGATTAATAA AAATACCTGA AAACCAATTG 
GTGCTGCTGA TAAATGACAG TTCAACTGGT ATCAAAGAAT TGAATCAATA TAAAACTCCA 
ATCAGTGAAT TTTTAAATGA AAATGATGAG ATAGAAGCTA TTGAAACTCC TAAATTAGAC 
ATGAATCAAA ACTGTAATTC AGGATCTCCC AAATCAACCT CAACAATATC CCAACAATTA 
ACCCTTGTAT GGACTAATCG TGTCGGAATT GGTAGTTCTG ATAAAAACAA ATTTTTTGGA 
CCTTATTTTA GTTGCACAAT CAATCGAGAG GCAAGTTTCA AAGATATACA GAGTGAATTA 
GTGAAAACAA TGCAACCGAT TTTGAGGTTA ACCTCTGTGT CAAGTTGTAT CAAAGATTTA 
CCCTTGTTTA AATTGAGAGT TGTTGGTGGC ATCCAAGATC GATGTTATTT ACCCGAGCAT 
GGTGATCATC CTTTATACTC TTCCACTGTC AACGAAGCAT TAGCTCATTG TAAAGAGAAA 
GATTATCGTG GACCAACCTA TGTGATGTTG ATTGTTGAAT GGGATTCTGA TTTAAAGCAA 
ACACTGGTCG TTGATGATGA TGAGGCATTA ATTGTTACCG AGGATCCAAA CATGGAATAT 
GTTATTGCTC GTAATCGTCT ATCTAACCAG GCAACTTTAG AAGATTGTTT CGACATGTAT 
TTCCGTGAAG AGAAACTTGG TGCTGATGAT GCTTGGATGT GTCCATCTTG TAAAAAGAAG 
CAGCAATGTA TTAAGAAACT TAGTCTCTGG TCAACCCCTG ATATACTTAT TATTCATCTA 
AAACGCTTCC GTCAAGCAAC TAATCTTCAA CGAACCAAAT TATCAACTTT AGTCAACTTT 
CCATTCACTG GTTTGGATAT GAATCCCTAC TTGAGTAAAC GCAAAGCTAA TCTAGACGAA 
TACAAAGAAT CTAATGGGGA TGTTAATAAT TTATCTTACA TTTTGTATCC TTGGAAAAAA 
CCGCGAGAAA AAGAGGTTCT TCCTTATTAT TCGGAAGATA ATATCTATGA TTTATATGCT 
GTCTGTAATC ACCATGGGAA CATGTCATCA GGACATTATA CAGCTTACTG TCGTGACATT 
AATGATCATA AATGGTATTT ATTTGATGAT CAACAAGTTA CTAAGGTTTC AGAAAACTCA 
TTAGTTACTG GAGATGCTTA CATCTTATTT TACCAACGAA GTACTATTCC ACCATTTACT 
CTTCCTCCTT CAGAATCACC GTCTATGAGA ACAAAACGAG CATCGTCGTC ATCATCAACA 
TCGTCATCGT CATCATCTTC ATCATCTTCC TCCTCTTCAT CTTCTTCATC ATTATCATCC 
AACGCGGATC AATCCTCAAC TAATTCATTG ACATCTCAAT CTAACGTCAG CTTAAATAAT 
GACAATCAAT CTTCACCGGT TAAATTAGCA AACTCAACTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015787758.1
CDS	142..3366
Translation	MVEKIINRFGEETTASIKQVMDGSGLAQAIGLPTSTSTSPASSTSSSTATSSLGKLIEVNFLDKSLHLDSDKIYQFKEVSKLNGLKQFSDQYSIEQLKIDLNNKDIESSCDLLVKDQQQHHHQHFHHHLNSQHQKQPQQSNSSNQPNQENNPQPSPHLHHQPISNPSCVTISDDDLREESKLPVINGTVNEDHDSSLKSASPQSEPASIPICVDKLEKPCIINSNENGIERPKLNQDVARQAVTLPRTQGQTHKDTSTLRRNGENDKKRSRSFIVRLLSNLPYLSISGSRLNGKDDDEDSSTDSYKSLRRSRERSNNHQSTNKLSNSMSSQDAVNLFTCNCSEGTHGKTTNQTKSKPLTLSGIKNHGNTCFMNAILQCLSHTDLLIEYFNDTQFYYKDLITRQQLNLCKNGSSSEVTAQCATLFQSLWKNNYSPDLSSKFKSLIGKQCKQYEDGGQHDAQEFLFFLLDIFHEDLNVAIKKKAKKFKIPSTWNRPDEVIAAETLSIYTRCDNSFIRKVFQAQLRSSVVCRSCKSHSNTFDPYMCLSLPVPTKLTHICIINVSYLDNVPKKIKMAVTIETQATLKEMRHKISRLIKIPENQLVLLINDSSTGIKELNQYKTPISEFLNENDEIEAIETPKLDMNQNCNSGSPKSTSTISQQLTLVWTNRVGIGSSDKNKFFGPYFSCTINREASFKDIQSELVKTMQPILRLTSVSSCIKDLPLFKLRVVGGIQDRCYLPEHGDHPLYSSTVNEALAHCKEKDYRGPTYVMLIVEWDSDLKQTLVVDDDEALIVTEDPNMEYVIARNRLSNQATLEDCFDMYFREEKLGADDAWMCPSCKKKQQCIKKLSLWSTPDILIIHLKRFRQATNLQRTKLSTLVNFPFTGLDMNPYLSKRKANLDEYKESNGDVNNLSYILYPWKKPREKEVLPYYSEDNIYDLYAVCNHHGNMSSGHYTAYCRDINDHKWYLFDDQQVTKVSENSLVTGDAYILFYQRSTIPPFTLPPSESPSMRTKRASSSSSTSSSSSSSSSSSSSSSSSLSSNADQSSTNSLTSQSNVSLNNDNQSSPVKLANSTL

Target ORF information:

RefSeq Version	XM_015932272.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae ubiquitin carboxyl-terminal hydrolase 31-like (LOC107364833), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015932272.1

ORF Insert Sequence:

ATGGTCGAGA AAATTATCAA TCGTTTTGGC GAAGAGACCA CAGCAAGTAT TAAACAGGTC 
ATGGATGGTT CAGGCCTCGC CCAAGCTATT GGTCTACCAA CATCAACATC AACATCACCA 
GCTTCATCTA CTTCATCATC AACCGCAACT TCATCCCTTG GTAAATTGAT TGAGGTTAAT 
TTCTTAGATA AATCTCTTCA CCTTGACAGT GATAAAATTT ACCAATTTAA AGAGGTTTCA 
AAGTTAAATG GTTTGAAACA GTTTTCTGAC CAATACTCTA TTGAGCAACT TAAGATTGAT 
CTTAATAATA AAGATATAGA ATCTAGTTGT GACCTCTTGG TTAAAGATCA GCAACAACAT 
CATCATCAAC ATTTTCATCA TCATCTAAAC TCTCAACATC AAAAGCAACC ACAGCAATCA 
AACTCTTCAA ACCAACCAAA CCAGGAAAAT AATCCTCAAC CATCTCCTCA TCTACATCAT 
CAACCCATTA GTAATCCATC ATGTGTTACA ATTAGTGATG ATGACTTAAG AGAGGAAAGT 
AAACTTCCAG TCATCAATGG TACAGTAAAT GAGGATCATG ATAGTTCACT GAAATCAGCC 
TCACCCCAGT CAGAACCAGC TTCTATTCCC ATTTGTGTGG ATAAACTTGA AAAACCATGT 
ATTATTAATT CAAATGAAAA TGGCATTGAA AGGCCTAAGC TAAACCAAGA CGTTGCTCGA 
CAAGCTGTAA CACTACCAAG AACTCAAGGA CAAACCCACA AAGATACATC TACCTTACGT 
AGAAATGGTG AAAATGATAA AAAGCGTTCT AGAAGTTTTA TAGTTCGTTT ATTATCAAAT 
TTGCCATATC TTTCAATTTC CGGGTCTCGT TTAAACGGCA AAGACGATGA CGAGGATTCA 
TCTACCGACA GTTATAAAAG TCTACGAAGA TCTAGAGAAC GATCAAATAA TCATCAATCG 
ACAAATAAAT TATCTAATTC AATGTCAAGC CAAGATGCTG TAAATCTATT CACATGTAAT 
TGTTCAGAGG GAACTCATGG TAAAACAACT AATCAAACTA AATCGAAGCC GTTAACCTTG 
TCTGGAATTA AAAACCATGG CAATACTTGT TTCATGAATG CTATTCTACA ATGTTTATCT 
CATACCGATT TACTTATAGA ATATTTTAAT GATACCCAAT TTTATTATAA AGATTTAATT 
ACACGACAAC AGCTCAATCT GTGTAAAAAT GGCTCAAGTA GTGAAGTCAC CGCTCAATGT 
GCAACTTTAT TTCAATCTTT ATGGAAAAAC AATTATTCTC CTGATTTATC TAGCAAATTT 
AAATCTTTAA TTGGTAAACA ATGTAAGCAA TATGAGGATG GTGGTCAACA TGATGCACAA 
GAGTTTCTAT TTTTTTTGTT GGACATTTTC CATGAAGATC TCAACGTTGC CATCAAAAAG 
AAGGCCAAAA AGTTCAAGAT TCCCAGTACC TGGAACCGGC CTGATGAAGT TATAGCCGCG 
GAAACTTTAT CGATTTATAC AAGATGTGAC AATAGTTTTA TTCGCAAAGT TTTTCAAGCT 
CAATTACGAT CATCGGTTGT TTGTCGTTCA TGTAAAAGCC ATAGCAATAC ATTTGACCCG 
TATATGTGCC TTTCATTACC GGTTCCCACC AAGCTTACCC ACATCTGTAT CATTAACGTT 
TCATACCTTG ATAATGTTCC AAAAAAGATC AAAATGGCTG TTACCATCGA GACTCAAGCT 
ACGCTTAAAG AAATGAGACA TAAAATTTCA AGATTAATAA AAATACCTGA AAACCAATTG 
GTGCTGCTGA TAAATGACAG TTCAACTGGT ATCAAAGAAT TGAATCAATA TAAAACTCCA 
ATCAGTGAAT TTTTAAATGA AAATGATGAG ATAGAAGCTA TTGAAACTCC TAAATTAGAC 
ATGAATCAAA ACTGTAATTC AGGATCTCCC AAATCAACCT CAACAATATC CCAACAATTA 
ACCCTTGTAT GGACTAATCG TGTCGGAATT GGTAGTTCTG ATAAAAACAA ATTTTTTGGA 
CCTTATTTTA GTTGCACAAT CAATCGAGAG GCAAGTTTCA AAGATATACA GAGTGAATTA 
GTGAAAACAA TGCAACCGAT TTTGAGGTTA ACCTCTGTGT CAAGTTGTAT CAAAGATTTA 
CCCTTGTTTA AATTGAGAGT TGTTGGTGGC ATCCAAGATC GATGTTATTT ACCCGAGCAT 
GGTGATCATC CTTTATACTC TTCCACTGTC AACGAAGCAT TAGCTCATTG TAAAGAGAAA 
GATTATCGTG GACCAACCTA TGTGATGTTG ATTGTTGAAT GGGATTCTGA TTTAAAGCAA 
ACACTGGTCG TTGATGATGA TGAGGCATTA ATTGTTACCG AGGATCCAAA CATGGAATAT 
GTTATTGCTC GTAATCGTCT ATCTAACCAG GCAACTTTAG AAGATTGTTT CGACATGTAT 
TTCCGTGAAG AGAAACTTGG TGCTGATGAT GCTTGGATGT GTCCATCTTG TAAAAAGAAG 
CAGCAATGTA TTAAGAAACT TAGTCTCTGG TCAACCCCTG ATATACTTAT TATTCATCTA 
AAACGCTTCC GTCAAGCAAC TAATCTTCAA CGAACCAAAT TATCAACTTT AGTCAACTTT 
CCATTCACTG GTTTGGATAT GAATCCCTAC TTGAGTAAAC GCAAAGCTAA TCTAGACGAA 
TACAAAGAAT CTAATGGGGA TGTTAATAAT TTATCTTACA TTTTGTATCC TTGGAAAAAA 
CCGCGAGAAA AAGAGGTTCT TCCTTATTAT TCGGAAGATA ATATCTATGA TTTATATGCT 
GTCTGTAATC ACCATGGGAA CATGTCATCA GGACATTATA CAGCTTACTG TCGTGACATT 
AATGATCATA AATGGTATTT ATTTGATGAT CAACAAGTTA CTAAGGTTTC AGAAAACTCA 
TTAGTTACTG GAGATGCTTA CATCTTATTT TACCAACGAA GTACTATTCC ACCATTTACT 
CTTCCTCCTT CAGAATCACC GTCTATGAGA ACAAAACGAG CATCGTCGTC ATCATCAACA 
TCGTCATCGT CATCATCTTC ATCATCTTCC TCCTCTTCAT CTTCTTCATC ATTATCATCC 
AACGCGGATC AATCCTCAAC TAATTCATTG ACATCTCAAT CTAACGTCAG CTTAAATAAT 
GACAATCAAT CTTCACCGGT TAAATTAGCA AACTCAACTT TATAA