LOC107364063 gene cDNA ORF clone, Tetranychus urticae(two-spotted spider mite)

mRNA	Protein	Name
XM_015931358.1	XP_015786844.1	calpain-8-like isoform X2
XM_015931359.1	XP_015786845.1	calpain-B-like isoform X3
XM_015931361.1	XP_015786847.1	calpain-B-like isoform X5
XM_015931362.1	XP_015786848.1	calpain-B-like isoform X6
XM_015931360.1	XP_015786846.1	calpain-B-like isoform X4
XM_015931360.2	XP_015786846.1	calpain-B isoform X4
XM_015931361.2	XP_015786847.1	calpain-B isoform X5
XM_015931358.2	XP_015786844.1	calpain-8 isoform X2
XM_015931359.2	XP_015786845.1	calpain-B isoform X3
XM_015931362.2	XP_015786848.1	calpain-B isoform X6
XM_015931357.1	XP_015786843.1	calpain-8-like isoform X1
XM_015931357.2	XP_015786843.1	calpain-8 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTe20242	XM_015931357.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTe20243	XM_015931358.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTe20244	XM_015931359.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20245	XM_015931360.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20246	XM_015931361.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20247	XM_015931362.2 Latest version!	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OTe20245	XM_015931360.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20246	XM_015931361.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20243	XM_015931358.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTe20244	XM_015931359.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20247	XM_015931362.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTe20242	XM_015931357.1	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

LOC107364063 ( XM_015931357.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786843.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTe20242
Clone ID Related Accession (Same CDS sequence)	XM_015931357.1 , XM_015931357.2
Accession Version	XM_015931357.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-8-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGC TATTGAATTC 
AAGTTCGATG GCTTCTCCTT GGATACATGT CGATCTATGA TAGCTTTACT CGATGATGAC 
AGATCAGGTA AACTCGGTTT GGATGAATTC ACATCTCTAT GGACCAAAGT TAGACATTGG 
TGTGATGTCT TCAAAAGCCA TGACAAAGAT TCAAGTGGAA CCATTAACAC TGCAGAGCTT 
CGTGCTGCTC TTCACGAAAC TGGCCTGTCT GTTAATCGGC AAATACTGAT TCTCATTGTT 
CATCGTTATG GAGATATTAG TGGCAAAAAA CACGATAATT CAGTGAAACA CGAGAGACAT 
TTATCATTTG ATAACTTTAT CCACGTTTGC TTAAAATTGA AACACAGTAT TGAAATTTGG 
AACAATCAAA ACTCATCTTT TGCTCGTTCG CTACCAAAAG TCTCCTCAGG AGCTTTATTC 
ACTCTCGATG AGTGGGTTGA AAAAGTAATG TACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786843.1
CDS	747..3365
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931357.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931357.1

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGC TATTGAATTC 
AAGTTCGATG GCTTCTCCTT GGATACATGT CGATCTATGA TAGCTTTACT CGATGATGAC 
AGATCAGGTA AACTCGGTTT GGATGAATTC ACATCTCTAT GGACCAAAGT TAGACATTGG 
TGTGATGTCT TCAAAAGCCA TGACAAAGAT TCAAGTGGAA CCATTAACAC TGCAGAGCTT 
CGTGCTGCTC TTCACGAAAC TGGCCTGTCT GTTAATCGGC AAATACTGAT TCTCATTGTT 
CATCGTTATG GAGATATTAG TGGCAAAAAA CACGATAATT CAGTGAAACA CGAGAGACAT 
TTATCATTTG ATAACTTTAT CCACGTTTGC TTAAAATTGA AACACAGTAT TGAAATTTGG 
AACAATCAAA ACTCATCTTT TGCTCGTTCG CTACCAAAAG TCTCCTCAGG AGCTTTATTC 
ACTCTCGATG AGTGGGTTGA AAAAGTAATG TACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931357.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786843.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTe20242
Clone ID Related Accession (Same CDS sequence)	XM_015931357.1 , XM_015931357.2
Accession Version	XM_015931357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGC TATTGAATTC 
AAGTTCGATG GCTTCTCCTT GGATACATGT CGATCTATGA TAGCTTTACT CGATGATGAC 
AGATCAGGTA AACTCGGTTT GGATGAATTC ACATCTCTAT GGACCAAAGT TAGACATTGG 
TGTGATGTCT TCAAAAGCCA TGACAAAGAT TCAAGTGGAA CCATTAACAC TGCAGAGCTT 
CGTGCTGCTC TTCACGAAAC TGGCCTGTCT GTTAATCGGC AAATACTGAT TCTCATTGTT 
CATCGTTATG GAGATATTAG TGGCAAAAAA CACGATAATT CAGTGAAACA CGAGAGACAT 
TTATCATTTG ATAACTTTAT CCACGTTTGC TTAAAATTGA AACACAGTAT TGAAATTTGG 
AACAATCAAA ACTCATCTTT TGCTCGTTCG CTACCAAAAG TCTCCTCAGG AGCTTTATTC 
ACTCTCGATG AGTGGGTTGA AAAAGTAATG TACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786843.1
CDS	747..3365
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931357.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931357.2

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGC TATTGAATTC 
AAGTTCGATG GCTTCTCCTT GGATACATGT CGATCTATGA TAGCTTTACT CGATGATGAC 
AGATCAGGTA AACTCGGTTT GGATGAATTC ACATCTCTAT GGACCAAAGT TAGACATTGG 
TGTGATGTCT TCAAAAGCCA TGACAAAGAT TCAAGTGGAA CCATTAACAC TGCAGAGCTT 
CGTGCTGCTC TTCACGAAAC TGGCCTGTCT GTTAATCGGC AAATACTGAT TCTCATTGTT 
CATCGTTATG GAGATATTAG TGGCAAAAAA CACGATAATT CAGTGAAACA CGAGAGACAT 
TTATCATTTG ATAACTTTAT CCACGTTTGC TTAAAATTGA AACACAGTAT TGAAATTTGG 
AACAATCAAA ACTCATCTTT TGCTCGTTCG CTACCAAAAG TCTCCTCAGG AGCTTTATTC 
ACTCTCGATG AGTGGGTTGA AAAAGTAATG TACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931358.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786844.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTe20243
Clone ID Related Accession (Same CDS sequence)	XM_015931358.1 , XM_015931358.2
Accession Version	XM_015931358.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-8-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGA ATTCAAGTTC 
GATGGCTTCT CCTTGGATAC ATGTCGATCT ATGATAGCTT TACTCGATGA TGACAGATCA 
GGTAAACTCG GTTTGGATGA ATTCACATCT CTATGGACCA AAGTTAGACA TTGGTGTGAT 
GTCTTCAAAA GCCATGACAA AGATTCAAGT GGAACCATTA ACACTGCAGA GCTTCGTGCT 
GCTCTTCACG AAACTGGCCT GTCTGTTAAT CGGCAAATAC TGATTCTCAT TGTTCATCGT 
TATGGAGATA TTAGTGGCAA AAAACACGAT AATTCAGTGA AACACGAGAG ACATTTATCA 
TTTGATAACT TTATCCACGT TTGCTTAAAA TTGAAACACA GTATTGAAAT TTGGAACAAT 
CAAAACTCAT CTTTTGCTCG TTCGCTACCA AAAGTCTCCT CAGGAGCTTT ATTCACTCTC 
GATGAGTGGG TTGAAAAAGT AATGTACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786844.1
CDS	747..3359
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931358.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931358.1

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGA ATTCAAGTTC 
GATGGCTTCT CCTTGGATAC ATGTCGATCT ATGATAGCTT TACTCGATGA TGACAGATCA 
GGTAAACTCG GTTTGGATGA ATTCACATCT CTATGGACCA AAGTTAGACA TTGGTGTGAT 
GTCTTCAAAA GCCATGACAA AGATTCAAGT GGAACCATTA ACACTGCAGA GCTTCGTGCT 
GCTCTTCACG AAACTGGCCT GTCTGTTAAT CGGCAAATAC TGATTCTCAT TGTTCATCGT 
TATGGAGATA TTAGTGGCAA AAAACACGAT AATTCAGTGA AACACGAGAG ACATTTATCA 
TTTGATAACT TTATCCACGT TTGCTTAAAA TTGAAACACA GTATTGAAAT TTGGAACAAT 
CAAAACTCAT CTTTTGCTCG TTCGCTACCA AAAGTCTCCT CAGGAGCTTT ATTCACTCTC 
GATGAGTGGG TTGAAAAAGT AATGTACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931358.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786844.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTe20243
Clone ID Related Accession (Same CDS sequence)	XM_015931358.1 , XM_015931358.2
Accession Version	XM_015931358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGA ATTCAAGTTC 
GATGGCTTCT CCTTGGATAC ATGTCGATCT ATGATAGCTT TACTCGATGA TGACAGATCA 
GGTAAACTCG GTTTGGATGA ATTCACATCT CTATGGACCA AAGTTAGACA TTGGTGTGAT 
GTCTTCAAAA GCCATGACAA AGATTCAAGT GGAACCATTA ACACTGCAGA GCTTCGTGCT 
GCTCTTCACG AAACTGGCCT GTCTGTTAAT CGGCAAATAC TGATTCTCAT TGTTCATCGT 
TATGGAGATA TTAGTGGCAA AAAACACGAT AATTCAGTGA AACACGAGAG ACATTTATCA 
TTTGATAACT TTATCCACGT TTGCTTAAAA TTGAAACACA GTATTGAAAT TTGGAACAAT 
CAAAACTCAT CTTTTGCTCG TTCGCTACCA AAAGTCTCCT CAGGAGCTTT ATTCACTCTC 
GATGAGTGGG TTGAAAAAGT AATGTACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786844.1
CDS	747..3359
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931358.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931358.2

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGTCGC TGAAGAAGAT 
ATTATTCGCC GTGAAGTTAA ACCACCTTTA CCAGGTCCAG GCGCAGGAAT ACTCGATGTT 
CAAAACATAA CCCGTTATAT TGAGATATTT GATACAGTCA TGAATGGTCT TCGTTTTGTG 
TTAACAAACT GTGTCCAAAG TGAAGAAACA CAAGAATCCG TTCAACAATC ACAGGGAACA 
AAAGTTTTAT CGACCAAAAC AGCCGATTTC TTTTCTAAAA TCGCTGGTGA AGACATGGAA 
GTTGATGCTG CTGAATTACA AGAAATACTT AATCATGCAC TCAAGAAAGA ATTCAAGTTC 
GATGGCTTCT CCTTGGATAC ATGTCGATCT ATGATAGCTT TACTCGATGA TGACAGATCA 
GGTAAACTCG GTTTGGATGA ATTCACATCT CTATGGACCA AAGTTAGACA TTGGTGTGAT 
GTCTTCAAAA GCCATGACAA AGATTCAAGT GGAACCATTA ACACTGCAGA GCTTCGTGCT 
GCTCTTCACG AAACTGGCCT GTCTGTTAAT CGGCAAATAC TGATTCTCAT TGTTCATCGT 
TATGGAGATA TTAGTGGCAA AAAACACGAT AATTCAGTGA AACACGAGAG ACATTTATCA 
TTTGATAACT TTATCCACGT TTGCTTAAAA TTGAAACACA GTATTGAAAT TTGGAACAAT 
CAAAACTCAT CTTTTGCTCG TTCGCTACCA AAAGTCTCCT CAGGAGCTTT ATTCACTCTC 
GATGAGTGGG TTGAAAAAGT AATGTACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931359.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786845.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTe20244
Clone ID Related Accession (Same CDS sequence)	XM_015931359.1 , XM_015931359.2
Accession Version	XM_015931359.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGAATC CGTTCAACAA 
TCACAGGGAA CAAAAGTTTT ATCGACCAAA ACAGCCGATT TCTTTTCTAA AATCGCTGGT 
GAAGACATGG AAGTTGATGC TGCTGAATTA CAAGAAATAC TTAATCATGC ACTCAAGAAA 
GCTATTGAAT TCAAGTTCGA TGGCTTCTCC TTGGATACAT GTCGATCTAT GATAGCTTTA 
CTCGATGATG ACAGATCAGG TAAACTCGGT TTGGATGAAT TCACATCTCT ATGGACCAAA 
GTTAGACATT GGTGTGATGT CTTCAAAAGC CATGACAAAG ATTCAAGTGG AACCATTAAC 
ACTGCAGAGC TTCGTGCTGC TCTTCACGAA ACTGGCCTGT CTGTTAATCG GCAAATACTG 
ATTCTCATTG TTCATCGTTA TGGAGATATT AGTGGCAAAA AACACGATAA TTCAGTGAAA 
CACGAGAGAC ATTTATCATT TGATAACTTT ATCCACGTTT GCTTAAAATT GAAACACAGT 
ATTGAAATTT GGAACAATCA AAACTCATCT TTTGCTCGTT CGCTACCAAA AGTCTCCTCA 
GGAGCTTTAT TCACTCTCGA TGAGTGGGTT GAAAAAGTAA TGTACTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786845.1
CDS	747..3197
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931359.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931359.1

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGAATC CGTTCAACAA 
TCACAGGGAA CAAAAGTTTT ATCGACCAAA ACAGCCGATT TCTTTTCTAA AATCGCTGGT 
GAAGACATGG AAGTTGATGC TGCTGAATTA CAAGAAATAC TTAATCATGC ACTCAAGAAA 
GCTATTGAAT TCAAGTTCGA TGGCTTCTCC TTGGATACAT GTCGATCTAT GATAGCTTTA 
CTCGATGATG ACAGATCAGG TAAACTCGGT TTGGATGAAT TCACATCTCT ATGGACCAAA 
GTTAGACATT GGTGTGATGT CTTCAAAAGC CATGACAAAG ATTCAAGTGG AACCATTAAC 
ACTGCAGAGC TTCGTGCTGC TCTTCACGAA ACTGGCCTGT CTGTTAATCG GCAAATACTG 
ATTCTCATTG TTCATCGTTA TGGAGATATT AGTGGCAAAA AACACGATAA TTCAGTGAAA 
CACGAGAGAC ATTTATCATT TGATAACTTT ATCCACGTTT GCTTAAAATT GAAACACAGT 
ATTGAAATTT GGAACAATCA AAACTCATCT TTTGCTCGTT CGCTACCAAA AGTCTCCTCA 
GGAGCTTTAT TCACTCTCGA TGAGTGGGTT GAAAAAGTAA TGTACTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931359.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786845.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTe20244
Clone ID Related Accession (Same CDS sequence)	XM_015931359.1 , XM_015931359.2
Accession Version	XM_015931359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGAATC CGTTCAACAA 
TCACAGGGAA CAAAAGTTTT ATCGACCAAA ACAGCCGATT TCTTTTCTAA AATCGCTGGT 
GAAGACATGG AAGTTGATGC TGCTGAATTA CAAGAAATAC TTAATCATGC ACTCAAGAAA 
GCTATTGAAT TCAAGTTCGA TGGCTTCTCC TTGGATACAT GTCGATCTAT GATAGCTTTA 
CTCGATGATG ACAGATCAGG TAAACTCGGT TTGGATGAAT TCACATCTCT ATGGACCAAA 
GTTAGACATT GGTGTGATGT CTTCAAAAGC CATGACAAAG ATTCAAGTGG AACCATTAAC 
ACTGCAGAGC TTCGTGCTGC TCTTCACGAA ACTGGCCTGT CTGTTAATCG GCAAATACTG 
ATTCTCATTG TTCATCGTTA TGGAGATATT AGTGGCAAAA AACACGATAA TTCAGTGAAA 
CACGAGAGAC ATTTATCATT TGATAACTTT ATCCACGTTT GCTTAAAATT GAAACACAGT 
ATTGAAATTT GGAACAATCA AAACTCATCT TTTGCTCGTT CGCTACCAAA AGTCTCCTCA 
GGAGCTTTAT TCACTCTCGA TGAGTGGGTT GAAAAAGTAA TGTACTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786845.1
CDS	747..3197
Translation	MGNQLTQCIELANSAASVLSPDGKSSTAASFIGPDTLKKLNVNEEVIQDGIKALSELVLKPKLKPAPAPRKPKDIHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931359.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931359.2

ORF Insert Sequence:

ATGGGCAATC AGTTGACCCA GTGCATTGAA TTGGCTAATA GCGCAGCATC AGTGTTATCA 
CCAGATGGAA AAAGTTCAAC AGCAGCCTCA TTCATTGGAC CAGACACACT AAAAAAATTA 
AATGTTAATG AAGAGGTGAT CCAAGACGGA ATAAAGGCTT TAAGTGAGCT AGTTCTAAAG 
CCTAAACTTA AGCCTGCTCC AGCTCCTAGA AAACCAAAAG ATATTCACAA TTTTGGTGAA 
CATGGATCTG GATTCCGAGC TCCTAATCCC GATGCCATTC AAGATTTCCA CAAAATTCGT 
GACCAATGTC TCCAAAGTGG ACAATTGTTT GAAGATAAAT CGTTTCCAGC CATTGGAGCA 
TCTCTATTTT TCTCAAGTCG TAAAGGCAAC GAATTCAAAT GGATGAGACC TAAAGAATTA 
GTCGATGATC CCAAATTCAT TTTGGAAGGA GCTTCACGTT TCGATGTTTG TCAAGGCGAG 
CTCGGTGATT GTTGGCTTCT TGCTTCAATT GCTAATTTGA CACTCAATGA GAAACTCTTC 
AGGAGAGTTG TTCCTCATGA TCAATCTTTT GGTTCCGATT ATGCTGGAAT CTTTCATTTC 
CGTTTCTGGC AATATGGTAA ATGGATTGAT ATTTGCGTTG ATGATCGATT ACCAACCATG 
AATGGAAAGC TTGTTCTCAT GCATTCAAAG GATAGGAATG AATTCTGGAG CGCACTTTTA 
GAGAAAGCTT ATGCAAAATT GCATGGCAGT TATGAAGCCT TAAAAGGAGG AACTGTTACA 
GAGGCGCTAA CTGATTTCAC TGGTGGTTTG ACTGAATTCT ATGAGATTCA ATCAGATGAA 
TGTCCAGCCA ATCTGCTTCA AATAATGACT AAAGCGCACC AAAGATCTTC TTTTATGGGC 
TGTTCTATTG ATGCTCTTGA CGCTTCTTCA ATGGAATCAG AATTACCAAA TGGTTTGATC 
AAAGGACATG CATACTCTAT AACAGCAGTT CGATGTGTTG ACCTAAAAAC TTCTCGGGTT 
CAAGGAAAAA TTCCCTTGAT TCGTATACGA AATCCATGGG GAAATGAAGC TGAATGGAAA 
GGGCCTTGGG GTGACGGATC ACGAGAGTGG ACTTTGGTTT CTGAGGAAGA AAAGAAAAGT 
CTTGAATTAA ATTTCGATCA CGATGGTGAA TTTTGGATGA CATTCCAAGA TTTCAAAGCT 
AACTTCACTA AACTTGAAAT TTGTCATCTC AAACCTGAGC CTACTGGTAC AAATGAGAGC 
AAAAAGGTTT GGAGTGAAGT GCATTTTGAA GGTGAATGGG TTGCTGGTGT AACTGCTGGA 
GGTTGTCGTA ATAATATCCA AACATTTGCC AGTAATCCTC AGTACATAAT TGAACTAAAA 
GACCATGATG AGGATGATGA AGATGATCTT TGTACTCTTA TTGTTGGTCT TATGCAAAAA 
AATCGTCGTG CTAAACGTAA AATGGGTCTC GATTCATTGA CCATTGGGTT TGCTGTTTAC 
GCAATCAAAG ATGGCCTTTT TTCTTATGGA GATGAGCCAG CTGCTGTTCG AGGTTATGCT 
CGTCGTCAAT TGCTACCAAC AGAGTTTTTC AAGTACAACG CTTCAGTTGC CAGATCTCCT 
ACTTTCATCA ATTTGAGAGA AGTTTCATCT CGTATGAGGC TTCCTCCCGG TCAATATTGT 
ATTATACCGT CCACATTTGA TCCCAATGAG GAAGGTGAAT TTCTGCTGCG TATATTCACC 
GAGAGGCCAC CCTCAACTGC CGAAGAAAAC GATGACGAAG TTGGTTATTG CCCAGAAGAT 
ATTGATCAAC ATAATGTTAT TGCCATCGAT GACTCGAAAG ACAAAGAATC CGTTCAACAA 
TCACAGGGAA CAAAAGTTTT ATCGACCAAA ACAGCCGATT TCTTTTCTAA AATCGCTGGT 
GAAGACATGG AAGTTGATGC TGCTGAATTA CAAGAAATAC TTAATCATGC ACTCAAGAAA 
GCTATTGAAT TCAAGTTCGA TGGCTTCTCC TTGGATACAT GTCGATCTAT GATAGCTTTA 
CTCGATGATG ACAGATCAGG TAAACTCGGT TTGGATGAAT TCACATCTCT ATGGACCAAA 
GTTAGACATT GGTGTGATGT CTTCAAAAGC CATGACAAAG ATTCAAGTGG AACCATTAAC 
ACTGCAGAGC TTCGTGCTGC TCTTCACGAA ACTGGCCTGT CTGTTAATCG GCAAATACTG 
ATTCTCATTG TTCATCGTTA TGGAGATATT AGTGGCAAAA AACACGATAA TTCAGTGAAA 
CACGAGAGAC ATTTATCATT TGATAACTTT ATCCACGTTT GCTTAAAATT GAAACACAGT 
ATTGAAATTT GGAACAATCA AAACTCATCT TTTGCTCGTT CGCTACCAAA AGTCTCCTCA 
GGAGCTTTAT TCACTCTCGA TGAGTGGGTT GAAAAAGTAA TGTACTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931360.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786846.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTe20245
Clone ID Related Accession (Same CDS sequence)	XM_015931360.1 , XM_015931360.2
Accession Version	XM_015931360.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGC CACATTTCTA CAAGTTTGGT GAACATGGAT CTGGATTCCG AGCTCCTAAT 
CCCGATGCCA TTCAAGATTT CCACAAAATT CGTGACCAAT GTCTCCAAAG TGGACAATTG 
TTTGAAGATA AATCGTTTCC AGCCATTGGA GCATCTCTAT TTTTCTCAAG TCGTAAAGGC 
AACGAATTCA AATGGATGAG ACCTAAAGAA TTAGTCGATG ATCCCAAATT CATTTTGGAA 
GGAGCTTCAC GTTTCGATGT TTGTCAAGGC GAGCTCGGTG ATTGTTGGCT TCTTGCTTCA 
ATTGCTAATT TGACACTCAA TGAGAAACTC TTCAGGAGAG TTGTTCCTCA TGATCAATCT 
TTTGGTTCCG ATTATGCTGG AATCTTTCAT TTCCGTTTCT GGCAATATGG TAAATGGATT 
GATATTTGCG TTGATGATCG ATTACCAACC ATGAATGGAA AGCTTGTTCT CATGCATTCA 
AAGGATAGGA ATGAATTCTG GAGCGCACTT TTAGAGAAAG CTTATGCAAA ATTGCATGGC 
AGTTATGAAG CCTTAAAAGG AGGAACTGTT ACAGAGGCGC TAACTGATTT CACTGGTGGT 
TTGACTGAAT TCTATGAGAT TCAATCAGAT GAATGTCCAG CCAATCTGCT TCAAATAATG 
ACTAAAGCGC ACCAAAGATC TTCTTTTATG GGCTGTTCTA TTGATGCTCT TGACGCTTCT 
TCAATGGAAT CAGAATTACC AAATGGTTTG ATCAAAGGAC ATGCATACTC TATAACAGCA 
GTTCGATGTG TTGACCTAAA AACTTCTCGG GTTCAAGGAA AAATTCCCTT GATTCGTATA 
CGAAATCCAT GGGGAAATGA AGCTGAATGG AAAGGGCCTT GGGGTGACGG ATCACGAGAG 
TGGACTTTGG TTTCTGAGGA AGAAAAGAAA AGTCTTGAAT TAAATTTCGA TCACGATGGT 
GAATTTTGGA TGACATTCCA AGATTTCAAA GCTAACTTCA CTAAACTTGA AATTTGTCAT 
CTCAAACCTG AGCCTACTGG TACAAATGAG AGCAAAAAGG TTTGGAGTGA AGTGCATTTT 
GAAGGTGAAT GGGTTGCTGG TGTAACTGCT GGAGGTTGTC GTAATAATAT CCAAACATTT 
GCCAGTAATC CTCAGTACAT AATTGAACTA AAAGACCATG ATGAGGATGA TGAAGATGAT 
CTTTGTACTC TTATTGTTGG TCTTATGCAA AAAAATCGTC GTGCTAAACG TAAAATGGGT 
CTCGATTCAT TGACCATTGG GTTTGCTGTT TACGCAATCA AAGATGGCCT TTTTTCTTAT 
GGAGATGAGC CAGCTGCTGT TCGAGGTTAT GCTCGTCGTC AATTGCTACC AACAGAGTTT 
TTCAAGTACA ACGCTTCAGT TGCCAGATCT CCTACTTTCA TCAATTTGAG AGAAGTTTCA 
TCTCGTATGA GGCTTCCTCC CGGTCAATAT TGTATTATAC CGTCCACATT TGATCCCAAT 
GAGGAAGGTG AATTTCTGCT GCGTATATTC ACCGAGAGGC CACCCTCAAC TGCCGAAGAA 
AACGATGACG AAGTTGGTTA TTGCCCAGAA GATATTGATC AACATAATGT TATTGCCATC 
GATGACTCGA AAGACAAAGT CGCTGAAGAA GATATTATTC GCCGTGAAGT TAAACCACCT 
TTACCAGGTC CAGGCGCAGG AATACTCGAT GTTCAAAACA TAACCCGTTA TATTGAGATA 
TTTGATACAG TCATGAATGG TCTTCGTTTT GTGTTAACAA ACTGTGTCCA AAGTGAAGAA 
ACACAAGAAT CCGTTCAACA ATCACAGGGA ACAAAAGTTT TATCGACCAA AACAGCCGAT 
TTCTTTTCTA AAATCGCTGG TGAAGACATG GAAGTTGATG CTGCTGAATT ACAAGAAATA 
CTTAATCATG CACTCAAGAA AGCTATTGAA TTCAAGTTCG ATGGCTTCTC CTTGGATACA 
TGTCGATCTA TGATAGCTTT ACTCGATGAT GACAGATCAG GTAAACTCGG TTTGGATGAA 
TTCACATCTC TATGGACCAA AGTTAGACAT TGGTGTGATG TCTTCAAAAG CCATGACAAA 
GATTCAAGTG GAACCATTAA CACTGCAGAG CTTCGTGCTG CTCTTCACGA AACTGGCCTG 
TCTGTTAATC GGCAAATACT GATTCTCATT GTTCATCGTT ATGGAGATAT TAGTGGCAAA 
AAACACGATA ATTCAGTGAA ACACGAGAGA CATTTATCAT TTGATAACTT TATCCACGTT 
TGCTTAAAAT TGAAACACAG TATTGAAATT TGGAACAATC AAAACTCATC TTTTGCTCGT 
TCGCTACCAA AAGTCTCCTC AGGAGCTTTA TTCACTCTCG ATGAGTGGGT TGAAAAAGTA 
ATGTACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786846.1
CDS	1058..3469
Translation	MGEPHFYKFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931360.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931360.1

ORF Insert Sequence:

ATGGGAGAGC CACATTTCTA CAAGTTTGGT GAACATGGAT CTGGATTCCG AGCTCCTAAT 
CCCGATGCCA TTCAAGATTT CCACAAAATT CGTGACCAAT GTCTCCAAAG TGGACAATTG 
TTTGAAGATA AATCGTTTCC AGCCATTGGA GCATCTCTAT TTTTCTCAAG TCGTAAAGGC 
AACGAATTCA AATGGATGAG ACCTAAAGAA TTAGTCGATG ATCCCAAATT CATTTTGGAA 
GGAGCTTCAC GTTTCGATGT TTGTCAAGGC GAGCTCGGTG ATTGTTGGCT TCTTGCTTCA 
ATTGCTAATT TGACACTCAA TGAGAAACTC TTCAGGAGAG TTGTTCCTCA TGATCAATCT 
TTTGGTTCCG ATTATGCTGG AATCTTTCAT TTCCGTTTCT GGCAATATGG TAAATGGATT 
GATATTTGCG TTGATGATCG ATTACCAACC ATGAATGGAA AGCTTGTTCT CATGCATTCA 
AAGGATAGGA ATGAATTCTG GAGCGCACTT TTAGAGAAAG CTTATGCAAA ATTGCATGGC 
AGTTATGAAG CCTTAAAAGG AGGAACTGTT ACAGAGGCGC TAACTGATTT CACTGGTGGT 
TTGACTGAAT TCTATGAGAT TCAATCAGAT GAATGTCCAG CCAATCTGCT TCAAATAATG 
ACTAAAGCGC ACCAAAGATC TTCTTTTATG GGCTGTTCTA TTGATGCTCT TGACGCTTCT 
TCAATGGAAT CAGAATTACC AAATGGTTTG ATCAAAGGAC ATGCATACTC TATAACAGCA 
GTTCGATGTG TTGACCTAAA AACTTCTCGG GTTCAAGGAA AAATTCCCTT GATTCGTATA 
CGAAATCCAT GGGGAAATGA AGCTGAATGG AAAGGGCCTT GGGGTGACGG ATCACGAGAG 
TGGACTTTGG TTTCTGAGGA AGAAAAGAAA AGTCTTGAAT TAAATTTCGA TCACGATGGT 
GAATTTTGGA TGACATTCCA AGATTTCAAA GCTAACTTCA CTAAACTTGA AATTTGTCAT 
CTCAAACCTG AGCCTACTGG TACAAATGAG AGCAAAAAGG TTTGGAGTGA AGTGCATTTT 
GAAGGTGAAT GGGTTGCTGG TGTAACTGCT GGAGGTTGTC GTAATAATAT CCAAACATTT 
GCCAGTAATC CTCAGTACAT AATTGAACTA AAAGACCATG ATGAGGATGA TGAAGATGAT 
CTTTGTACTC TTATTGTTGG TCTTATGCAA AAAAATCGTC GTGCTAAACG TAAAATGGGT 
CTCGATTCAT TGACCATTGG GTTTGCTGTT TACGCAATCA AAGATGGCCT TTTTTCTTAT 
GGAGATGAGC CAGCTGCTGT TCGAGGTTAT GCTCGTCGTC AATTGCTACC AACAGAGTTT 
TTCAAGTACA ACGCTTCAGT TGCCAGATCT CCTACTTTCA TCAATTTGAG AGAAGTTTCA 
TCTCGTATGA GGCTTCCTCC CGGTCAATAT TGTATTATAC CGTCCACATT TGATCCCAAT 
GAGGAAGGTG AATTTCTGCT GCGTATATTC ACCGAGAGGC CACCCTCAAC TGCCGAAGAA 
AACGATGACG AAGTTGGTTA TTGCCCAGAA GATATTGATC AACATAATGT TATTGCCATC 
GATGACTCGA AAGACAAAGT CGCTGAAGAA GATATTATTC GCCGTGAAGT TAAACCACCT 
TTACCAGGTC CAGGCGCAGG AATACTCGAT GTTCAAAACA TAACCCGTTA TATTGAGATA 
TTTGATACAG TCATGAATGG TCTTCGTTTT GTGTTAACAA ACTGTGTCCA AAGTGAAGAA 
ACACAAGAAT CCGTTCAACA ATCACAGGGA ACAAAAGTTT TATCGACCAA AACAGCCGAT 
TTCTTTTCTA AAATCGCTGG TGAAGACATG GAAGTTGATG CTGCTGAATT ACAAGAAATA 
CTTAATCATG CACTCAAGAA AGCTATTGAA TTCAAGTTCG ATGGCTTCTC CTTGGATACA 
TGTCGATCTA TGATAGCTTT ACTCGATGAT GACAGATCAG GTAAACTCGG TTTGGATGAA 
TTCACATCTC TATGGACCAA AGTTAGACAT TGGTGTGATG TCTTCAAAAG CCATGACAAA 
GATTCAAGTG GAACCATTAA CACTGCAGAG CTTCGTGCTG CTCTTCACGA AACTGGCCTG 
TCTGTTAATC GGCAAATACT GATTCTCATT GTTCATCGTT ATGGAGATAT TAGTGGCAAA 
AAACACGATA ATTCAGTGAA ACACGAGAGA CATTTATCAT TTGATAACTT TATCCACGTT 
TGCTTAAAAT TGAAACACAG TATTGAAATT TGGAACAATC AAAACTCATC TTTTGCTCGT 
TCGCTACCAA AAGTCTCCTC AGGAGCTTTA TTCACTCTCG ATGAGTGGGT TGAAAAAGTA 
ATGTACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931360.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786846.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTe20245
Clone ID Related Accession (Same CDS sequence)	XM_015931360.1 , XM_015931360.2
Accession Version	XM_015931360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGC CACATTTCTA CAAGTTTGGT GAACATGGAT CTGGATTCCG AGCTCCTAAT 
CCCGATGCCA TTCAAGATTT CCACAAAATT CGTGACCAAT GTCTCCAAAG TGGACAATTG 
TTTGAAGATA AATCGTTTCC AGCCATTGGA GCATCTCTAT TTTTCTCAAG TCGTAAAGGC 
AACGAATTCA AATGGATGAG ACCTAAAGAA TTAGTCGATG ATCCCAAATT CATTTTGGAA 
GGAGCTTCAC GTTTCGATGT TTGTCAAGGC GAGCTCGGTG ATTGTTGGCT TCTTGCTTCA 
ATTGCTAATT TGACACTCAA TGAGAAACTC TTCAGGAGAG TTGTTCCTCA TGATCAATCT 
TTTGGTTCCG ATTATGCTGG AATCTTTCAT TTCCGTTTCT GGCAATATGG TAAATGGATT 
GATATTTGCG TTGATGATCG ATTACCAACC ATGAATGGAA AGCTTGTTCT CATGCATTCA 
AAGGATAGGA ATGAATTCTG GAGCGCACTT TTAGAGAAAG CTTATGCAAA ATTGCATGGC 
AGTTATGAAG CCTTAAAAGG AGGAACTGTT ACAGAGGCGC TAACTGATTT CACTGGTGGT 
TTGACTGAAT TCTATGAGAT TCAATCAGAT GAATGTCCAG CCAATCTGCT TCAAATAATG 
ACTAAAGCGC ACCAAAGATC TTCTTTTATG GGCTGTTCTA TTGATGCTCT TGACGCTTCT 
TCAATGGAAT CAGAATTACC AAATGGTTTG ATCAAAGGAC ATGCATACTC TATAACAGCA 
GTTCGATGTG TTGACCTAAA AACTTCTCGG GTTCAAGGAA AAATTCCCTT GATTCGTATA 
CGAAATCCAT GGGGAAATGA AGCTGAATGG AAAGGGCCTT GGGGTGACGG ATCACGAGAG 
TGGACTTTGG TTTCTGAGGA AGAAAAGAAA AGTCTTGAAT TAAATTTCGA TCACGATGGT 
GAATTTTGGA TGACATTCCA AGATTTCAAA GCTAACTTCA CTAAACTTGA AATTTGTCAT 
CTCAAACCTG AGCCTACTGG TACAAATGAG AGCAAAAAGG TTTGGAGTGA AGTGCATTTT 
GAAGGTGAAT GGGTTGCTGG TGTAACTGCT GGAGGTTGTC GTAATAATAT CCAAACATTT 
GCCAGTAATC CTCAGTACAT AATTGAACTA AAAGACCATG ATGAGGATGA TGAAGATGAT 
CTTTGTACTC TTATTGTTGG TCTTATGCAA AAAAATCGTC GTGCTAAACG TAAAATGGGT 
CTCGATTCAT TGACCATTGG GTTTGCTGTT TACGCAATCA AAGATGGCCT TTTTTCTTAT 
GGAGATGAGC CAGCTGCTGT TCGAGGTTAT GCTCGTCGTC AATTGCTACC AACAGAGTTT 
TTCAAGTACA ACGCTTCAGT TGCCAGATCT CCTACTTTCA TCAATTTGAG AGAAGTTTCA 
TCTCGTATGA GGCTTCCTCC CGGTCAATAT TGTATTATAC CGTCCACATT TGATCCCAAT 
GAGGAAGGTG AATTTCTGCT GCGTATATTC ACCGAGAGGC CACCCTCAAC TGCCGAAGAA 
AACGATGACG AAGTTGGTTA TTGCCCAGAA GATATTGATC AACATAATGT TATTGCCATC 
GATGACTCGA AAGACAAAGT CGCTGAAGAA GATATTATTC GCCGTGAAGT TAAACCACCT 
TTACCAGGTC CAGGCGCAGG AATACTCGAT GTTCAAAACA TAACCCGTTA TATTGAGATA 
TTTGATACAG TCATGAATGG TCTTCGTTTT GTGTTAACAA ACTGTGTCCA AAGTGAAGAA 
ACACAAGAAT CCGTTCAACA ATCACAGGGA ACAAAAGTTT TATCGACCAA AACAGCCGAT 
TTCTTTTCTA AAATCGCTGG TGAAGACATG GAAGTTGATG CTGCTGAATT ACAAGAAATA 
CTTAATCATG CACTCAAGAA AGCTATTGAA TTCAAGTTCG ATGGCTTCTC CTTGGATACA 
TGTCGATCTA TGATAGCTTT ACTCGATGAT GACAGATCAG GTAAACTCGG TTTGGATGAA 
TTCACATCTC TATGGACCAA AGTTAGACAT TGGTGTGATG TCTTCAAAAG CCATGACAAA 
GATTCAAGTG GAACCATTAA CACTGCAGAG CTTCGTGCTG CTCTTCACGA AACTGGCCTG 
TCTGTTAATC GGCAAATACT GATTCTCATT GTTCATCGTT ATGGAGATAT TAGTGGCAAA 
AAACACGATA ATTCAGTGAA ACACGAGAGA CATTTATCAT TTGATAACTT TATCCACGTT 
TGCTTAAAAT TGAAACACAG TATTGAAATT TGGAACAATC AAAACTCATC TTTTGCTCGT 
TCGCTACCAA AAGTCTCCTC AGGAGCTTTA TTCACTCTCG ATGAGTGGGT TGAAAAAGTA 
ATGTACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786846.1
CDS	1077..3488
Translation	MGEPHFYKFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931360.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931360.2

ORF Insert Sequence:

ATGGGAGAGC CACATTTCTA CAAGTTTGGT GAACATGGAT CTGGATTCCG AGCTCCTAAT 
CCCGATGCCA TTCAAGATTT CCACAAAATT CGTGACCAAT GTCTCCAAAG TGGACAATTG 
TTTGAAGATA AATCGTTTCC AGCCATTGGA GCATCTCTAT TTTTCTCAAG TCGTAAAGGC 
AACGAATTCA AATGGATGAG ACCTAAAGAA TTAGTCGATG ATCCCAAATT CATTTTGGAA 
GGAGCTTCAC GTTTCGATGT TTGTCAAGGC GAGCTCGGTG ATTGTTGGCT TCTTGCTTCA 
ATTGCTAATT TGACACTCAA TGAGAAACTC TTCAGGAGAG TTGTTCCTCA TGATCAATCT 
TTTGGTTCCG ATTATGCTGG AATCTTTCAT TTCCGTTTCT GGCAATATGG TAAATGGATT 
GATATTTGCG TTGATGATCG ATTACCAACC ATGAATGGAA AGCTTGTTCT CATGCATTCA 
AAGGATAGGA ATGAATTCTG GAGCGCACTT TTAGAGAAAG CTTATGCAAA ATTGCATGGC 
AGTTATGAAG CCTTAAAAGG AGGAACTGTT ACAGAGGCGC TAACTGATTT CACTGGTGGT 
TTGACTGAAT TCTATGAGAT TCAATCAGAT GAATGTCCAG CCAATCTGCT TCAAATAATG 
ACTAAAGCGC ACCAAAGATC TTCTTTTATG GGCTGTTCTA TTGATGCTCT TGACGCTTCT 
TCAATGGAAT CAGAATTACC AAATGGTTTG ATCAAAGGAC ATGCATACTC TATAACAGCA 
GTTCGATGTG TTGACCTAAA AACTTCTCGG GTTCAAGGAA AAATTCCCTT GATTCGTATA 
CGAAATCCAT GGGGAAATGA AGCTGAATGG AAAGGGCCTT GGGGTGACGG ATCACGAGAG 
TGGACTTTGG TTTCTGAGGA AGAAAAGAAA AGTCTTGAAT TAAATTTCGA TCACGATGGT 
GAATTTTGGA TGACATTCCA AGATTTCAAA GCTAACTTCA CTAAACTTGA AATTTGTCAT 
CTCAAACCTG AGCCTACTGG TACAAATGAG AGCAAAAAGG TTTGGAGTGA AGTGCATTTT 
GAAGGTGAAT GGGTTGCTGG TGTAACTGCT GGAGGTTGTC GTAATAATAT CCAAACATTT 
GCCAGTAATC CTCAGTACAT AATTGAACTA AAAGACCATG ATGAGGATGA TGAAGATGAT 
CTTTGTACTC TTATTGTTGG TCTTATGCAA AAAAATCGTC GTGCTAAACG TAAAATGGGT 
CTCGATTCAT TGACCATTGG GTTTGCTGTT TACGCAATCA AAGATGGCCT TTTTTCTTAT 
GGAGATGAGC CAGCTGCTGT TCGAGGTTAT GCTCGTCGTC AATTGCTACC AACAGAGTTT 
TTCAAGTACA ACGCTTCAGT TGCCAGATCT CCTACTTTCA TCAATTTGAG AGAAGTTTCA 
TCTCGTATGA GGCTTCCTCC CGGTCAATAT TGTATTATAC CGTCCACATT TGATCCCAAT 
GAGGAAGGTG AATTTCTGCT GCGTATATTC ACCGAGAGGC CACCCTCAAC TGCCGAAGAA 
AACGATGACG AAGTTGGTTA TTGCCCAGAA GATATTGATC AACATAATGT TATTGCCATC 
GATGACTCGA AAGACAAAGT CGCTGAAGAA GATATTATTC GCCGTGAAGT TAAACCACCT 
TTACCAGGTC CAGGCGCAGG AATACTCGAT GTTCAAAACA TAACCCGTTA TATTGAGATA 
TTTGATACAG TCATGAATGG TCTTCGTTTT GTGTTAACAA ACTGTGTCCA AAGTGAAGAA 
ACACAAGAAT CCGTTCAACA ATCACAGGGA ACAAAAGTTT TATCGACCAA AACAGCCGAT 
TTCTTTTCTA AAATCGCTGG TGAAGACATG GAAGTTGATG CTGCTGAATT ACAAGAAATA 
CTTAATCATG CACTCAAGAA AGCTATTGAA TTCAAGTTCG ATGGCTTCTC CTTGGATACA 
TGTCGATCTA TGATAGCTTT ACTCGATGAT GACAGATCAG GTAAACTCGG TTTGGATGAA 
TTCACATCTC TATGGACCAA AGTTAGACAT TGGTGTGATG TCTTCAAAAG CCATGACAAA 
GATTCAAGTG GAACCATTAA CACTGCAGAG CTTCGTGCTG CTCTTCACGA AACTGGCCTG 
TCTGTTAATC GGCAAATACT GATTCTCATT GTTCATCGTT ATGGAGATAT TAGTGGCAAA 
AAACACGATA ATTCAGTGAA ACACGAGAGA CATTTATCAT TTGATAACTT TATCCACGTT 
TGCTTAAAAT TGAAACACAG TATTGAAATT TGGAACAATC AAAACTCATC TTTTGCTCGT 
TCGCTACCAA AAGTCTCCTC AGGAGCTTTA TTCACTCTCG ATGAGTGGGT TGAAAAAGTA 
ATGTACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931361.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786847.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTe20246
Clone ID Related Accession (Same CDS sequence)	XM_015931361.1 , XM_015931361.2
Accession Version	XM_015931361.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTC ATAATTTTGG TGAACATGGA TCTGGATTCC GAGCTCCTAA TCCCGATGCC 
ATTCAAGATT TCCACAAAAT TCGTGACCAA TGTCTCCAAA GTGGACAATT GTTTGAAGAT 
AAATCGTTTC CAGCCATTGG AGCATCTCTA TTTTTCTCAA GTCGTAAAGG CAACGAATTC 
AAATGGATGA GACCTAAAGA ATTAGTCGAT GATCCCAAAT TCATTTTGGA AGGAGCTTCA 
CGTTTCGATG TTTGTCAAGG CGAGCTCGGT GATTGTTGGC TTCTTGCTTC AATTGCTAAT 
TTGACACTCA ATGAGAAACT CTTCAGGAGA GTTGTTCCTC ATGATCAATC TTTTGGTTCC 
GATTATGCTG GAATCTTTCA TTTCCGTTTC TGGCAATATG GTAAATGGAT TGATATTTGC 
GTTGATGATC GATTACCAAC CATGAATGGA AAGCTTGTTC TCATGCATTC AAAGGATAGG 
AATGAATTCT GGAGCGCACT TTTAGAGAAA GCTTATGCAA AATTGCATGG CAGTTATGAA 
GCCTTAAAAG GAGGAACTGT TACAGAGGCG CTAACTGATT TCACTGGTGG TTTGACTGAA 
TTCTATGAGA TTCAATCAGA TGAATGTCCA GCCAATCTGC TTCAAATAAT GACTAAAGCG 
CACCAAAGAT CTTCTTTTAT GGGCTGTTCT ATTGATGCTC TTGACGCTTC TTCAATGGAA 
TCAGAATTAC CAAATGGTTT GATCAAAGGA CATGCATACT CTATAACAGC AGTTCGATGT 
GTTGACCTAA AAACTTCTCG GGTTCAAGGA AAAATTCCCT TGATTCGTAT ACGAAATCCA 
TGGGGAAATG AAGCTGAATG GAAAGGGCCT TGGGGTGACG GATCACGAGA GTGGACTTTG 
GTTTCTGAGG AAGAAAAGAA AAGTCTTGAA TTAAATTTCG ATCACGATGG TGAATTTTGG 
ATGACATTCC AAGATTTCAA AGCTAACTTC ACTAAACTTG AAATTTGTCA TCTCAAACCT 
GAGCCTACTG GTACAAATGA GAGCAAAAAG GTTTGGAGTG AAGTGCATTT TGAAGGTGAA 
TGGGTTGCTG GTGTAACTGC TGGAGGTTGT CGTAATAATA TCCAAACATT TGCCAGTAAT 
CCTCAGTACA TAATTGAACT AAAAGACCAT GATGAGGATG ATGAAGATGA TCTTTGTACT 
CTTATTGTTG GTCTTATGCA AAAAAATCGT CGTGCTAAAC GTAAAATGGG TCTCGATTCA 
TTGACCATTG GGTTTGCTGT TTACGCAATC AAAGATGGCC TTTTTTCTTA TGGAGATGAG 
CCAGCTGCTG TTCGAGGTTA TGCTCGTCGT CAATTGCTAC CAACAGAGTT TTTCAAGTAC 
AACGCTTCAG TTGCCAGATC TCCTACTTTC ATCAATTTGA GAGAAGTTTC ATCTCGTATG 
AGGCTTCCTC CCGGTCAATA TTGTATTATA CCGTCCACAT TTGATCCCAA TGAGGAAGGT 
GAATTTCTGC TGCGTATATT CACCGAGAGG CCACCCTCAA CTGCCGAAGA AAACGATGAC 
GAAGTTGGTT ATTGCCCAGA AGATATTGAT CAACATAATG TTATTGCCAT CGATGACTCG 
AAAGACAAAG TCGCTGAAGA AGATATTATT CGCCGTGAAG TTAAACCACC TTTACCAGGT 
CCAGGCGCAG GAATACTCGA TGTTCAAAAC ATAACCCGTT ATATTGAGAT ATTTGATACA 
GTCATGAATG GTCTTCGTTT TGTGTTAACA AACTGTGTCC AAAGTGAAGA AACACAAGAA 
TCCGTTCAAC AATCACAGGG AACAAAAGTT TTATCGACCA AAACAGCCGA TTTCTTTTCT 
AAAATCGCTG GTGAAGACAT GGAAGTTGAT GCTGCTGAAT TACAAGAAAT ACTTAATCAT 
GCACTCAAGA AAGCTATTGA ATTCAAGTTC GATGGCTTCT CCTTGGATAC ATGTCGATCT 
ATGATAGCTT TACTCGATGA TGACAGATCA GGTAAACTCG GTTTGGATGA ATTCACATCT 
CTATGGACCA AAGTTAGACA TTGGTGTGAT GTCTTCAAAA GCCATGACAA AGATTCAAGT 
GGAACCATTA ACACTGCAGA GCTTCGTGCT GCTCTTCACG AAACTGGCCT GTCTGTTAAT 
CGGCAAATAC TGATTCTCAT TGTTCATCGT TATGGAGATA TTAGTGGCAA AAAACACGAT 
AATTCAGTGA AACACGAGAG ACATTTATCA TTTGATAACT TTATCCACGT TTGCTTAAAA 
TTGAAACACA GTATTGAAAT TTGGAACAAT CAAAACTCAT CTTTTGCTCG TTCGCTACCA 
AAAGTCTCCT CAGGAGCTTT ATTCACTCTC GATGAGTGGG TTGAAAAAGT AATGTACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786847.1
CDS	214..2616
Translation	MAVHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931361.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931361.1

ORF Insert Sequence:

ATGGCTGTTC ATAATTTTGG TGAACATGGA TCTGGATTCC GAGCTCCTAA TCCCGATGCC 
ATTCAAGATT TCCACAAAAT TCGTGACCAA TGTCTCCAAA GTGGACAATT GTTTGAAGAT 
AAATCGTTTC CAGCCATTGG AGCATCTCTA TTTTTCTCAA GTCGTAAAGG CAACGAATTC 
AAATGGATGA GACCTAAAGA ATTAGTCGAT GATCCCAAAT TCATTTTGGA AGGAGCTTCA 
CGTTTCGATG TTTGTCAAGG CGAGCTCGGT GATTGTTGGC TTCTTGCTTC AATTGCTAAT 
TTGACACTCA ATGAGAAACT CTTCAGGAGA GTTGTTCCTC ATGATCAATC TTTTGGTTCC 
GATTATGCTG GAATCTTTCA TTTCCGTTTC TGGCAATATG GTAAATGGAT TGATATTTGC 
GTTGATGATC GATTACCAAC CATGAATGGA AAGCTTGTTC TCATGCATTC AAAGGATAGG 
AATGAATTCT GGAGCGCACT TTTAGAGAAA GCTTATGCAA AATTGCATGG CAGTTATGAA 
GCCTTAAAAG GAGGAACTGT TACAGAGGCG CTAACTGATT TCACTGGTGG TTTGACTGAA 
TTCTATGAGA TTCAATCAGA TGAATGTCCA GCCAATCTGC TTCAAATAAT GACTAAAGCG 
CACCAAAGAT CTTCTTTTAT GGGCTGTTCT ATTGATGCTC TTGACGCTTC TTCAATGGAA 
TCAGAATTAC CAAATGGTTT GATCAAAGGA CATGCATACT CTATAACAGC AGTTCGATGT 
GTTGACCTAA AAACTTCTCG GGTTCAAGGA AAAATTCCCT TGATTCGTAT ACGAAATCCA 
TGGGGAAATG AAGCTGAATG GAAAGGGCCT TGGGGTGACG GATCACGAGA GTGGACTTTG 
GTTTCTGAGG AAGAAAAGAA AAGTCTTGAA TTAAATTTCG ATCACGATGG TGAATTTTGG 
ATGACATTCC AAGATTTCAA AGCTAACTTC ACTAAACTTG AAATTTGTCA TCTCAAACCT 
GAGCCTACTG GTACAAATGA GAGCAAAAAG GTTTGGAGTG AAGTGCATTT TGAAGGTGAA 
TGGGTTGCTG GTGTAACTGC TGGAGGTTGT CGTAATAATA TCCAAACATT TGCCAGTAAT 
CCTCAGTACA TAATTGAACT AAAAGACCAT GATGAGGATG ATGAAGATGA TCTTTGTACT 
CTTATTGTTG GTCTTATGCA AAAAAATCGT CGTGCTAAAC GTAAAATGGG TCTCGATTCA 
TTGACCATTG GGTTTGCTGT TTACGCAATC AAAGATGGCC TTTTTTCTTA TGGAGATGAG 
CCAGCTGCTG TTCGAGGTTA TGCTCGTCGT CAATTGCTAC CAACAGAGTT TTTCAAGTAC 
AACGCTTCAG TTGCCAGATC TCCTACTTTC ATCAATTTGA GAGAAGTTTC ATCTCGTATG 
AGGCTTCCTC CCGGTCAATA TTGTATTATA CCGTCCACAT TTGATCCCAA TGAGGAAGGT 
GAATTTCTGC TGCGTATATT CACCGAGAGG CCACCCTCAA CTGCCGAAGA AAACGATGAC 
GAAGTTGGTT ATTGCCCAGA AGATATTGAT CAACATAATG TTATTGCCAT CGATGACTCG 
AAAGACAAAG TCGCTGAAGA AGATATTATT CGCCGTGAAG TTAAACCACC TTTACCAGGT 
CCAGGCGCAG GAATACTCGA TGTTCAAAAC ATAACCCGTT ATATTGAGAT ATTTGATACA 
GTCATGAATG GTCTTCGTTT TGTGTTAACA AACTGTGTCC AAAGTGAAGA AACACAAGAA 
TCCGTTCAAC AATCACAGGG AACAAAAGTT TTATCGACCA AAACAGCCGA TTTCTTTTCT 
AAAATCGCTG GTGAAGACAT GGAAGTTGAT GCTGCTGAAT TACAAGAAAT ACTTAATCAT 
GCACTCAAGA AAGCTATTGA ATTCAAGTTC GATGGCTTCT CCTTGGATAC ATGTCGATCT 
ATGATAGCTT TACTCGATGA TGACAGATCA GGTAAACTCG GTTTGGATGA ATTCACATCT 
CTATGGACCA AAGTTAGACA TTGGTGTGAT GTCTTCAAAA GCCATGACAA AGATTCAAGT 
GGAACCATTA ACACTGCAGA GCTTCGTGCT GCTCTTCACG AAACTGGCCT GTCTGTTAAT 
CGGCAAATAC TGATTCTCAT TGTTCATCGT TATGGAGATA TTAGTGGCAA AAAACACGAT 
AATTCAGTGA AACACGAGAG ACATTTATCA TTTGATAACT TTATCCACGT TTGCTTAAAA 
TTGAAACACA GTATTGAAAT TTGGAACAAT CAAAACTCAT CTTTTGCTCG TTCGCTACCA 
AAAGTCTCCT CAGGAGCTTT ATTCACTCTC GATGAGTGGG TTGAAAAAGT AATGTACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931361.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786847.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTe20246
Clone ID Related Accession (Same CDS sequence)	XM_015931361.1 , XM_015931361.2
Accession Version	XM_015931361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTC ATAATTTTGG TGAACATGGA TCTGGATTCC GAGCTCCTAA TCCCGATGCC 
ATTCAAGATT TCCACAAAAT TCGTGACCAA TGTCTCCAAA GTGGACAATT GTTTGAAGAT 
AAATCGTTTC CAGCCATTGG AGCATCTCTA TTTTTCTCAA GTCGTAAAGG CAACGAATTC 
AAATGGATGA GACCTAAAGA ATTAGTCGAT GATCCCAAAT TCATTTTGGA AGGAGCTTCA 
CGTTTCGATG TTTGTCAAGG CGAGCTCGGT GATTGTTGGC TTCTTGCTTC AATTGCTAAT 
TTGACACTCA ATGAGAAACT CTTCAGGAGA GTTGTTCCTC ATGATCAATC TTTTGGTTCC 
GATTATGCTG GAATCTTTCA TTTCCGTTTC TGGCAATATG GTAAATGGAT TGATATTTGC 
GTTGATGATC GATTACCAAC CATGAATGGA AAGCTTGTTC TCATGCATTC AAAGGATAGG 
AATGAATTCT GGAGCGCACT TTTAGAGAAA GCTTATGCAA AATTGCATGG CAGTTATGAA 
GCCTTAAAAG GAGGAACTGT TACAGAGGCG CTAACTGATT TCACTGGTGG TTTGACTGAA 
TTCTATGAGA TTCAATCAGA TGAATGTCCA GCCAATCTGC TTCAAATAAT GACTAAAGCG 
CACCAAAGAT CTTCTTTTAT GGGCTGTTCT ATTGATGCTC TTGACGCTTC TTCAATGGAA 
TCAGAATTAC CAAATGGTTT GATCAAAGGA CATGCATACT CTATAACAGC AGTTCGATGT 
GTTGACCTAA AAACTTCTCG GGTTCAAGGA AAAATTCCCT TGATTCGTAT ACGAAATCCA 
TGGGGAAATG AAGCTGAATG GAAAGGGCCT TGGGGTGACG GATCACGAGA GTGGACTTTG 
GTTTCTGAGG AAGAAAAGAA AAGTCTTGAA TTAAATTTCG ATCACGATGG TGAATTTTGG 
ATGACATTCC AAGATTTCAA AGCTAACTTC ACTAAACTTG AAATTTGTCA TCTCAAACCT 
GAGCCTACTG GTACAAATGA GAGCAAAAAG GTTTGGAGTG AAGTGCATTT TGAAGGTGAA 
TGGGTTGCTG GTGTAACTGC TGGAGGTTGT CGTAATAATA TCCAAACATT TGCCAGTAAT 
CCTCAGTACA TAATTGAACT AAAAGACCAT GATGAGGATG ATGAAGATGA TCTTTGTACT 
CTTATTGTTG GTCTTATGCA AAAAAATCGT CGTGCTAAAC GTAAAATGGG TCTCGATTCA 
TTGACCATTG GGTTTGCTGT TTACGCAATC AAAGATGGCC TTTTTTCTTA TGGAGATGAG 
CCAGCTGCTG TTCGAGGTTA TGCTCGTCGT CAATTGCTAC CAACAGAGTT TTTCAAGTAC 
AACGCTTCAG TTGCCAGATC TCCTACTTTC ATCAATTTGA GAGAAGTTTC ATCTCGTATG 
AGGCTTCCTC CCGGTCAATA TTGTATTATA CCGTCCACAT TTGATCCCAA TGAGGAAGGT 
GAATTTCTGC TGCGTATATT CACCGAGAGG CCACCCTCAA CTGCCGAAGA AAACGATGAC 
GAAGTTGGTT ATTGCCCAGA AGATATTGAT CAACATAATG TTATTGCCAT CGATGACTCG 
AAAGACAAAG TCGCTGAAGA AGATATTATT CGCCGTGAAG TTAAACCACC TTTACCAGGT 
CCAGGCGCAG GAATACTCGA TGTTCAAAAC ATAACCCGTT ATATTGAGAT ATTTGATACA 
GTCATGAATG GTCTTCGTTT TGTGTTAACA AACTGTGTCC AAAGTGAAGA AACACAAGAA 
TCCGTTCAAC AATCACAGGG AACAAAAGTT TTATCGACCA AAACAGCCGA TTTCTTTTCT 
AAAATCGCTG GTGAAGACAT GGAAGTTGAT GCTGCTGAAT TACAAGAAAT ACTTAATCAT 
GCACTCAAGA AAGCTATTGA ATTCAAGTTC GATGGCTTCT CCTTGGATAC ATGTCGATCT 
ATGATAGCTT TACTCGATGA TGACAGATCA GGTAAACTCG GTTTGGATGA ATTCACATCT 
CTATGGACCA AAGTTAGACA TTGGTGTGAT GTCTTCAAAA GCCATGACAA AGATTCAAGT 
GGAACCATTA ACACTGCAGA GCTTCGTGCT GCTCTTCACG AAACTGGCCT GTCTGTTAAT 
CGGCAAATAC TGATTCTCAT TGTTCATCGT TATGGAGATA TTAGTGGCAA AAAACACGAT 
AATTCAGTGA AACACGAGAG ACATTTATCA TTTGATAACT TTATCCACGT TTGCTTAAAA 
TTGAAACACA GTATTGAAAT TTGGAACAAT CAAAACTCAT CTTTTGCTCG TTCGCTACCA 
AAAGTCTCCT CAGGAGCTTT ATTCACTCTC GATGAGTGGG TTGAAAAAGT AATGTACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786847.1
CDS	213..2615
Translation	MAVHNFGEHGSGFRAPNPDAIQDFHKIRDQCLQSGQLFEDKSFPAIGASLFFSSRKGNEFKWMRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931361.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931361.2

ORF Insert Sequence:

ATGGCTGTTC ATAATTTTGG TGAACATGGA TCTGGATTCC GAGCTCCTAA TCCCGATGCC 
ATTCAAGATT TCCACAAAAT TCGTGACCAA TGTCTCCAAA GTGGACAATT GTTTGAAGAT 
AAATCGTTTC CAGCCATTGG AGCATCTCTA TTTTTCTCAA GTCGTAAAGG CAACGAATTC 
AAATGGATGA GACCTAAAGA ATTAGTCGAT GATCCCAAAT TCATTTTGGA AGGAGCTTCA 
CGTTTCGATG TTTGTCAAGG CGAGCTCGGT GATTGTTGGC TTCTTGCTTC AATTGCTAAT 
TTGACACTCA ATGAGAAACT CTTCAGGAGA GTTGTTCCTC ATGATCAATC TTTTGGTTCC 
GATTATGCTG GAATCTTTCA TTTCCGTTTC TGGCAATATG GTAAATGGAT TGATATTTGC 
GTTGATGATC GATTACCAAC CATGAATGGA AAGCTTGTTC TCATGCATTC AAAGGATAGG 
AATGAATTCT GGAGCGCACT TTTAGAGAAA GCTTATGCAA AATTGCATGG CAGTTATGAA 
GCCTTAAAAG GAGGAACTGT TACAGAGGCG CTAACTGATT TCACTGGTGG TTTGACTGAA 
TTCTATGAGA TTCAATCAGA TGAATGTCCA GCCAATCTGC TTCAAATAAT GACTAAAGCG 
CACCAAAGAT CTTCTTTTAT GGGCTGTTCT ATTGATGCTC TTGACGCTTC TTCAATGGAA 
TCAGAATTAC CAAATGGTTT GATCAAAGGA CATGCATACT CTATAACAGC AGTTCGATGT 
GTTGACCTAA AAACTTCTCG GGTTCAAGGA AAAATTCCCT TGATTCGTAT ACGAAATCCA 
TGGGGAAATG AAGCTGAATG GAAAGGGCCT TGGGGTGACG GATCACGAGA GTGGACTTTG 
GTTTCTGAGG AAGAAAAGAA AAGTCTTGAA TTAAATTTCG ATCACGATGG TGAATTTTGG 
ATGACATTCC AAGATTTCAA AGCTAACTTC ACTAAACTTG AAATTTGTCA TCTCAAACCT 
GAGCCTACTG GTACAAATGA GAGCAAAAAG GTTTGGAGTG AAGTGCATTT TGAAGGTGAA 
TGGGTTGCTG GTGTAACTGC TGGAGGTTGT CGTAATAATA TCCAAACATT TGCCAGTAAT 
CCTCAGTACA TAATTGAACT AAAAGACCAT GATGAGGATG ATGAAGATGA TCTTTGTACT 
CTTATTGTTG GTCTTATGCA AAAAAATCGT CGTGCTAAAC GTAAAATGGG TCTCGATTCA 
TTGACCATTG GGTTTGCTGT TTACGCAATC AAAGATGGCC TTTTTTCTTA TGGAGATGAG 
CCAGCTGCTG TTCGAGGTTA TGCTCGTCGT CAATTGCTAC CAACAGAGTT TTTCAAGTAC 
AACGCTTCAG TTGCCAGATC TCCTACTTTC ATCAATTTGA GAGAAGTTTC ATCTCGTATG 
AGGCTTCCTC CCGGTCAATA TTGTATTATA CCGTCCACAT TTGATCCCAA TGAGGAAGGT 
GAATTTCTGC TGCGTATATT CACCGAGAGG CCACCCTCAA CTGCCGAAGA AAACGATGAC 
GAAGTTGGTT ATTGCCCAGA AGATATTGAT CAACATAATG TTATTGCCAT CGATGACTCG 
AAAGACAAAG TCGCTGAAGA AGATATTATT CGCCGTGAAG TTAAACCACC TTTACCAGGT 
CCAGGCGCAG GAATACTCGA TGTTCAAAAC ATAACCCGTT ATATTGAGAT ATTTGATACA 
GTCATGAATG GTCTTCGTTT TGTGTTAACA AACTGTGTCC AAAGTGAAGA AACACAAGAA 
TCCGTTCAAC AATCACAGGG AACAAAAGTT TTATCGACCA AAACAGCCGA TTTCTTTTCT 
AAAATCGCTG GTGAAGACAT GGAAGTTGAT GCTGCTGAAT TACAAGAAAT ACTTAATCAT 
GCACTCAAGA AAGCTATTGA ATTCAAGTTC GATGGCTTCT CCTTGGATAC ATGTCGATCT 
ATGATAGCTT TACTCGATGA TGACAGATCA GGTAAACTCG GTTTGGATGA ATTCACATCT 
CTATGGACCA AAGTTAGACA TTGGTGTGAT GTCTTCAAAA GCCATGACAA AGATTCAAGT 
GGAACCATTA ACACTGCAGA GCTTCGTGCT GCTCTTCACG AAACTGGCCT GTCTGTTAAT 
CGGCAAATAC TGATTCTCAT TGTTCATCGT TATGGAGATA TTAGTGGCAA AAAACACGAT 
AATTCAGTGA AACACGAGAG ACATTTATCA TTTGATAACT TTATCCACGT TTGCTTAAAA 
TTGAAACACA GTATTGAAAT TTGGAACAAT CAAAACTCAT CTTTTGCTCG TTCGCTACCA 
AAAGTCTCCT CAGGAGCTTT ATTCACTCTC GATGAGTGGG TTGAAAAAGT AATGTACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931362.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786848.1 Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTe20247
Clone ID Related Accession (Same CDS sequence)	XM_015931362.1 , XM_015931362.2
Accession Version	XM_015931362.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTA AAGAATTAGT CGATGATCCC AAATTCATTT TGGAAGGAGC TTCACGTTTC 
GATGTTTGTC AAGGCGAGCT CGGTGATTGT TGGCTTCTTG CTTCAATTGC TAATTTGACA 
CTCAATGAGA AACTCTTCAG GAGAGTTGTT CCTCATGATC AATCTTTTGG TTCCGATTAT 
GCTGGAATCT TTCATTTCCG TTTCTGGCAA TATGGTAAAT GGATTGATAT TTGCGTTGAT 
GATCGATTAC CAACCATGAA TGGAAAGCTT GTTCTCATGC ATTCAAAGGA TAGGAATGAA 
TTCTGGAGCG CACTTTTAGA GAAAGCTTAT GCAAAATTGC ATGGCAGTTA TGAAGCCTTA 
AAAGGAGGAA CTGTTACAGA GGCGCTAACT GATTTCACTG GTGGTTTGAC TGAATTCTAT 
GAGATTCAAT CAGATGAATG TCCAGCCAAT CTGCTTCAAA TAATGACTAA AGCGCACCAA 
AGATCTTCTT TTATGGGCTG TTCTATTGAT GCTCTTGACG CTTCTTCAAT GGAATCAGAA 
TTACCAAATG GTTTGATCAA AGGACATGCA TACTCTATAA CAGCAGTTCG ATGTGTTGAC 
CTAAAAACTT CTCGGGTTCA AGGAAAAATT CCCTTGATTC GTATACGAAA TCCATGGGGA 
AATGAAGCTG AATGGAAAGG GCCTTGGGGT GACGGATCAC GAGAGTGGAC TTTGGTTTCT 
GAGGAAGAAA AGAAAAGTCT TGAATTAAAT TTCGATCACG ATGGTGAATT TTGGATGACA 
TTCCAAGATT TCAAAGCTAA CTTCACTAAA CTTGAAATTT GTCATCTCAA ACCTGAGCCT 
ACTGGTACAA ATGAGAGCAA AAAGGTTTGG AGTGAAGTGC ATTTTGAAGG TGAATGGGTT 
GCTGGTGTAA CTGCTGGAGG TTGTCGTAAT AATATCCAAA CATTTGCCAG TAATCCTCAG 
TACATAATTG AACTAAAAGA CCATGATGAG GATGATGAAG ATGATCTTTG TACTCTTATT 
GTTGGTCTTA TGCAAAAAAA TCGTCGTGCT AAACGTAAAA TGGGTCTCGA TTCATTGACC 
ATTGGGTTTG CTGTTTACGC AATCAAAGAT GGCCTTTTTT CTTATGGAGA TGAGCCAGCT 
GCTGTTCGAG GTTATGCTCG TCGTCAATTG CTACCAACAG AGTTTTTCAA GTACAACGCT 
TCAGTTGCCA GATCTCCTAC TTTCATCAAT TTGAGAGAAG TTTCATCTCG TATGAGGCTT 
CCTCCCGGTC AATATTGTAT TATACCGTCC ACATTTGATC CCAATGAGGA AGGTGAATTT 
CTGCTGCGTA TATTCACCGA GAGGCCACCC TCAACTGCCG AAGAAAACGA TGACGAAGTT 
GGTTATTGCC CAGAAGATAT TGATCAACAT AATGTTATTG CCATCGATGA CTCGAAAGAC 
AAAGTCGCTG AAGAAGATAT TATTCGCCGT GAAGTTAAAC CACCTTTACC AGGTCCAGGC 
GCAGGAATAC TCGATGTTCA AAACATAACC CGTTATATTG AGATATTTGA TACAGTCATG 
AATGGTCTTC GTTTTGTGTT AACAAACTGT GTCCAAAGTG AAGAAACACA AGAATCCGTT 
CAACAATCAC AGGGAACAAA AGTTTTATCG ACCAAAACAG CCGATTTCTT TTCTAAAATC 
GCTGGTGAAG ACATGGAAGT TGATGCTGCT GAATTACAAG AAATACTTAA TCATGCACTC 
AAGAAAGCTA TTGAATTCAA GTTCGATGGC TTCTCCTTGG ATACATGTCG ATCTATGATA 
GCTTTACTCG ATGATGACAG ATCAGGTAAA CTCGGTTTGG ATGAATTCAC ATCTCTATGG 
ACCAAAGTTA GACATTGGTG TGATGTCTTC AAAAGCCATG ACAAAGATTC AAGTGGAACC 
ATTAACACTG CAGAGCTTCG TGCTGCTCTT CACGAAACTG GCCTGTCTGT TAATCGGCAA 
ATACTGATTC TCATTGTTCA TCGTTATGGA GATATTAGTG GCAAAAAACA CGATAATTCA 
GTGAAACACG AGAGACATTT ATCATTTGAT AACTTTATCC ACGTTTGCTT AAAATTGAAA 
CACAGTATTG AAATTTGGAA CAATCAAAAC TCATCTTTTG CTCGTTCGCT ACCAAAAGTC 
TCCTCAGGAG CTTTATTCAC TCTCGATGAG TGGGTTGAAA AAGTAATGTA CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786848.1
CDS	434..2650
Translation	MRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931362.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8-like (LOC107364063), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931362.1

ORF Insert Sequence:

ATGAGACCTA AAGAATTAGT CGATGATCCC AAATTCATTT TGGAAGGAGC TTCACGTTTC 
GATGTTTGTC AAGGCGAGCT CGGTGATTGT TGGCTTCTTG CTTCAATTGC TAATTTGACA 
CTCAATGAGA AACTCTTCAG GAGAGTTGTT CCTCATGATC AATCTTTTGG TTCCGATTAT 
GCTGGAATCT TTCATTTCCG TTTCTGGCAA TATGGTAAAT GGATTGATAT TTGCGTTGAT 
GATCGATTAC CAACCATGAA TGGAAAGCTT GTTCTCATGC ATTCAAAGGA TAGGAATGAA 
TTCTGGAGCG CACTTTTAGA GAAAGCTTAT GCAAAATTGC ATGGCAGTTA TGAAGCCTTA 
AAAGGAGGAA CTGTTACAGA GGCGCTAACT GATTTCACTG GTGGTTTGAC TGAATTCTAT 
GAGATTCAAT CAGATGAATG TCCAGCCAAT CTGCTTCAAA TAATGACTAA AGCGCACCAA 
AGATCTTCTT TTATGGGCTG TTCTATTGAT GCTCTTGACG CTTCTTCAAT GGAATCAGAA 
TTACCAAATG GTTTGATCAA AGGACATGCA TACTCTATAA CAGCAGTTCG ATGTGTTGAC 
CTAAAAACTT CTCGGGTTCA AGGAAAAATT CCCTTGATTC GTATACGAAA TCCATGGGGA 
AATGAAGCTG AATGGAAAGG GCCTTGGGGT GACGGATCAC GAGAGTGGAC TTTGGTTTCT 
GAGGAAGAAA AGAAAAGTCT TGAATTAAAT TTCGATCACG ATGGTGAATT TTGGATGACA 
TTCCAAGATT TCAAAGCTAA CTTCACTAAA CTTGAAATTT GTCATCTCAA ACCTGAGCCT 
ACTGGTACAA ATGAGAGCAA AAAGGTTTGG AGTGAAGTGC ATTTTGAAGG TGAATGGGTT 
GCTGGTGTAA CTGCTGGAGG TTGTCGTAAT AATATCCAAA CATTTGCCAG TAATCCTCAG 
TACATAATTG AACTAAAAGA CCATGATGAG GATGATGAAG ATGATCTTTG TACTCTTATT 
GTTGGTCTTA TGCAAAAAAA TCGTCGTGCT AAACGTAAAA TGGGTCTCGA TTCATTGACC 
ATTGGGTTTG CTGTTTACGC AATCAAAGAT GGCCTTTTTT CTTATGGAGA TGAGCCAGCT 
GCTGTTCGAG GTTATGCTCG TCGTCAATTG CTACCAACAG AGTTTTTCAA GTACAACGCT 
TCAGTTGCCA GATCTCCTAC TTTCATCAAT TTGAGAGAAG TTTCATCTCG TATGAGGCTT 
CCTCCCGGTC AATATTGTAT TATACCGTCC ACATTTGATC CCAATGAGGA AGGTGAATTT 
CTGCTGCGTA TATTCACCGA GAGGCCACCC TCAACTGCCG AAGAAAACGA TGACGAAGTT 
GGTTATTGCC CAGAAGATAT TGATCAACAT AATGTTATTG CCATCGATGA CTCGAAAGAC 
AAAGTCGCTG AAGAAGATAT TATTCGCCGT GAAGTTAAAC CACCTTTACC AGGTCCAGGC 
GCAGGAATAC TCGATGTTCA AAACATAACC CGTTATATTG AGATATTTGA TACAGTCATG 
AATGGTCTTC GTTTTGTGTT AACAAACTGT GTCCAAAGTG AAGAAACACA AGAATCCGTT 
CAACAATCAC AGGGAACAAA AGTTTTATCG ACCAAAACAG CCGATTTCTT TTCTAAAATC 
GCTGGTGAAG ACATGGAAGT TGATGCTGCT GAATTACAAG AAATACTTAA TCATGCACTC 
AAGAAAGCTA TTGAATTCAA GTTCGATGGC TTCTCCTTGG ATACATGTCG ATCTATGATA 
GCTTTACTCG ATGATGACAG ATCAGGTAAA CTCGGTTTGG ATGAATTCAC ATCTCTATGG 
ACCAAAGTTA GACATTGGTG TGATGTCTTC AAAAGCCATG ACAAAGATTC AAGTGGAACC 
ATTAACACTG CAGAGCTTCG TGCTGCTCTT CACGAAACTG GCCTGTCTGT TAATCGGCAA 
ATACTGATTC TCATTGTTCA TCGTTATGGA GATATTAGTG GCAAAAAACA CGATAATTCA 
GTGAAACACG AGAGACATTT ATCATTTGAT AACTTTATCC ACGTTTGCTT AAAATTGAAA 
CACAGTATTG AAATTTGGAA CAATCAAAAC TCATCTTTTG CTCGTTCGCT ACCAAAAGTC 
TCCTCAGGAG CTTTATTCAC TCTCGATGAG TGGGTTGAAA AAGTAATGTA CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107364063 ( XM_015931362.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015786848.1 Tetranychus urticae calpain-8 (LOC107364063), transcript variant X6, mRNA.

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CloneID	OTe20247
Clone ID Related Accession (Same CDS sequence)	XM_015931362.1 , XM_015931362.2
Accession Version	XM_015931362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	calpain-B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449940.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015931362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTA AAGAATTAGT CGATGATCCC AAATTCATTT TGGAAGGAGC TTCACGTTTC 
GATGTTTGTC AAGGCGAGCT CGGTGATTGT TGGCTTCTTG CTTCAATTGC TAATTTGACA 
CTCAATGAGA AACTCTTCAG GAGAGTTGTT CCTCATGATC AATCTTTTGG TTCCGATTAT 
GCTGGAATCT TTCATTTCCG TTTCTGGCAA TATGGTAAAT GGATTGATAT TTGCGTTGAT 
GATCGATTAC CAACCATGAA TGGAAAGCTT GTTCTCATGC ATTCAAAGGA TAGGAATGAA 
TTCTGGAGCG CACTTTTAGA GAAAGCTTAT GCAAAATTGC ATGGCAGTTA TGAAGCCTTA 
AAAGGAGGAA CTGTTACAGA GGCGCTAACT GATTTCACTG GTGGTTTGAC TGAATTCTAT 
GAGATTCAAT CAGATGAATG TCCAGCCAAT CTGCTTCAAA TAATGACTAA AGCGCACCAA 
AGATCTTCTT TTATGGGCTG TTCTATTGAT GCTCTTGACG CTTCTTCAAT GGAATCAGAA 
TTACCAAATG GTTTGATCAA AGGACATGCA TACTCTATAA CAGCAGTTCG ATGTGTTGAC 
CTAAAAACTT CTCGGGTTCA AGGAAAAATT CCCTTGATTC GTATACGAAA TCCATGGGGA 
AATGAAGCTG AATGGAAAGG GCCTTGGGGT GACGGATCAC GAGAGTGGAC TTTGGTTTCT 
GAGGAAGAAA AGAAAAGTCT TGAATTAAAT TTCGATCACG ATGGTGAATT TTGGATGACA 
TTCCAAGATT TCAAAGCTAA CTTCACTAAA CTTGAAATTT GTCATCTCAA ACCTGAGCCT 
ACTGGTACAA ATGAGAGCAA AAAGGTTTGG AGTGAAGTGC ATTTTGAAGG TGAATGGGTT 
GCTGGTGTAA CTGCTGGAGG TTGTCGTAAT AATATCCAAA CATTTGCCAG TAATCCTCAG 
TACATAATTG AACTAAAAGA CCATGATGAG GATGATGAAG ATGATCTTTG TACTCTTATT 
GTTGGTCTTA TGCAAAAAAA TCGTCGTGCT AAACGTAAAA TGGGTCTCGA TTCATTGACC 
ATTGGGTTTG CTGTTTACGC AATCAAAGAT GGCCTTTTTT CTTATGGAGA TGAGCCAGCT 
GCTGTTCGAG GTTATGCTCG TCGTCAATTG CTACCAACAG AGTTTTTCAA GTACAACGCT 
TCAGTTGCCA GATCTCCTAC TTTCATCAAT TTGAGAGAAG TTTCATCTCG TATGAGGCTT 
CCTCCCGGTC AATATTGTAT TATACCGTCC ACATTTGATC CCAATGAGGA AGGTGAATTT 
CTGCTGCGTA TATTCACCGA GAGGCCACCC TCAACTGCCG AAGAAAACGA TGACGAAGTT 
GGTTATTGCC CAGAAGATAT TGATCAACAT AATGTTATTG CCATCGATGA CTCGAAAGAC 
AAAGTCGCTG AAGAAGATAT TATTCGCCGT GAAGTTAAAC CACCTTTACC AGGTCCAGGC 
GCAGGAATAC TCGATGTTCA AAACATAACC CGTTATATTG AGATATTTGA TACAGTCATG 
AATGGTCTTC GTTTTGTGTT AACAAACTGT GTCCAAAGTG AAGAAACACA AGAATCCGTT 
CAACAATCAC AGGGAACAAA AGTTTTATCG ACCAAAACAG CCGATTTCTT TTCTAAAATC 
GCTGGTGAAG ACATGGAAGT TGATGCTGCT GAATTACAAG AAATACTTAA TCATGCACTC 
AAGAAAGCTA TTGAATTCAA GTTCGATGGC TTCTCCTTGG ATACATGTCG ATCTATGATA 
GCTTTACTCG ATGATGACAG ATCAGGTAAA CTCGGTTTGG ATGAATTCAC ATCTCTATGG 
ACCAAAGTTA GACATTGGTG TGATGTCTTC AAAAGCCATG ACAAAGATTC AAGTGGAACC 
ATTAACACTG CAGAGCTTCG TGCTGCTCTT CACGAAACTG GCCTGTCTGT TAATCGGCAA 
ATACTGATTC TCATTGTTCA TCGTTATGGA GATATTAGTG GCAAAAAACA CGATAATTCA 
GTGAAACACG AGAGACATTT ATCATTTGAT AACTTTATCC ACGTTTGCTT AAAATTGAAA 
CACAGTATTG AAATTTGGAA CAATCAAAAC TCATCTTTTG CTCGTTCGCT ACCAAAAGTC 
TCCTCAGGAG CTTTATTCAC TCTCGATGAG TGGGTTGAAA AAGTAATGTA CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015786848.1
CDS	190..2406
Translation	MRPKELVDDPKFILEGASRFDVCQGELGDCWLLASIANLTLNEKLFRRVVPHDQSFGSDYAGIFHFRFWQYGKWIDICVDDRLPTMNGKLVLMHSKDRNEFWSALLEKAYAKLHGSYEALKGGTVTEALTDFTGGLTEFYEIQSDECPANLLQIMTKAHQRSSFMGCSIDALDASSMESELPNGLIKGHAYSITAVRCVDLKTSRVQGKIPLIRIRNPWGNEAEWKGPWGDGSREWTLVSEEEKKSLELNFDHDGEFWMTFQDFKANFTKLEICHLKPEPTGTNESKKVWSEVHFEGEWVAGVTAGGCRNNIQTFASNPQYIIELKDHDEDDEDDLCTLIVGLMQKNRRAKRKMGLDSLTIGFAVYAIKDGLFSYGDEPAAVRGYARRQLLPTEFFKYNASVARSPTFINLREVSSRMRLPPGQYCIIPSTFDPNEEGEFLLRIFTERPPSTAEENDDEVGYCPEDIDQHNVIAIDDSKDKVAEEDIIRREVKPPLPGPGAGILDVQNITRYIEIFDTVMNGLRFVLTNCVQSEETQESVQQSQGTKVLSTKTADFFSKIAGEDMEVDAAELQEILNHALKKAIEFKFDGFSLDTCRSMIALLDDDRSGKLGLDEFTSLWTKVRHWCDVFKSHDKDSSGTINTAELRAALHETGLSVNRQILILIVHRYGDISGKKHDNSVKHERHLSFDNFIHVCLKLKHSIEIWNNQNSSFARSLPKVSSGALFTLDEWVEKVMYC

Target ORF information:

RefSeq Version	XM_015931362.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae calpain-8 (LOC107364063), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015931362.2

ORF Insert Sequence:

ATGAGACCTA AAGAATTAGT CGATGATCCC AAATTCATTT TGGAAGGAGC TTCACGTTTC 
GATGTTTGTC AAGGCGAGCT CGGTGATTGT TGGCTTCTTG CTTCAATTGC TAATTTGACA 
CTCAATGAGA AACTCTTCAG GAGAGTTGTT CCTCATGATC AATCTTTTGG TTCCGATTAT 
GCTGGAATCT TTCATTTCCG TTTCTGGCAA TATGGTAAAT GGATTGATAT TTGCGTTGAT 
GATCGATTAC CAACCATGAA TGGAAAGCTT GTTCTCATGC ATTCAAAGGA TAGGAATGAA 
TTCTGGAGCG CACTTTTAGA GAAAGCTTAT GCAAAATTGC ATGGCAGTTA TGAAGCCTTA 
AAAGGAGGAA CTGTTACAGA GGCGCTAACT GATTTCACTG GTGGTTTGAC TGAATTCTAT 
GAGATTCAAT CAGATGAATG TCCAGCCAAT CTGCTTCAAA TAATGACTAA AGCGCACCAA 
AGATCTTCTT TTATGGGCTG TTCTATTGAT GCTCTTGACG CTTCTTCAAT GGAATCAGAA 
TTACCAAATG GTTTGATCAA AGGACATGCA TACTCTATAA CAGCAGTTCG ATGTGTTGAC 
CTAAAAACTT CTCGGGTTCA AGGAAAAATT CCCTTGATTC GTATACGAAA TCCATGGGGA 
AATGAAGCTG AATGGAAAGG GCCTTGGGGT GACGGATCAC GAGAGTGGAC TTTGGTTTCT 
GAGGAAGAAA AGAAAAGTCT TGAATTAAAT TTCGATCACG ATGGTGAATT TTGGATGACA 
TTCCAAGATT TCAAAGCTAA CTTCACTAAA CTTGAAATTT GTCATCTCAA ACCTGAGCCT 
ACTGGTACAA ATGAGAGCAA AAAGGTTTGG AGTGAAGTGC ATTTTGAAGG TGAATGGGTT 
GCTGGTGTAA CTGCTGGAGG TTGTCGTAAT AATATCCAAA CATTTGCCAG TAATCCTCAG 
TACATAATTG AACTAAAAGA CCATGATGAG GATGATGAAG ATGATCTTTG TACTCTTATT 
GTTGGTCTTA TGCAAAAAAA TCGTCGTGCT AAACGTAAAA TGGGTCTCGA TTCATTGACC 
ATTGGGTTTG CTGTTTACGC AATCAAAGAT GGCCTTTTTT CTTATGGAGA TGAGCCAGCT 
GCTGTTCGAG GTTATGCTCG TCGTCAATTG CTACCAACAG AGTTTTTCAA GTACAACGCT 
TCAGTTGCCA GATCTCCTAC TTTCATCAAT TTGAGAGAAG TTTCATCTCG TATGAGGCTT 
CCTCCCGGTC AATATTGTAT TATACCGTCC ACATTTGATC CCAATGAGGA AGGTGAATTT 
CTGCTGCGTA TATTCACCGA GAGGCCACCC TCAACTGCCG AAGAAAACGA TGACGAAGTT 
GGTTATTGCC CAGAAGATAT TGATCAACAT AATGTTATTG CCATCGATGA CTCGAAAGAC 
AAAGTCGCTG AAGAAGATAT TATTCGCCGT GAAGTTAAAC CACCTTTACC AGGTCCAGGC 
GCAGGAATAC TCGATGTTCA AAACATAACC CGTTATATTG AGATATTTGA TACAGTCATG 
AATGGTCTTC GTTTTGTGTT AACAAACTGT GTCCAAAGTG AAGAAACACA AGAATCCGTT 
CAACAATCAC AGGGAACAAA AGTTTTATCG ACCAAAACAG CCGATTTCTT TTCTAAAATC 
GCTGGTGAAG ACATGGAAGT TGATGCTGCT GAATTACAAG AAATACTTAA TCATGCACTC 
AAGAAAGCTA TTGAATTCAA GTTCGATGGC TTCTCCTTGG ATACATGTCG ATCTATGATA 
GCTTTACTCG ATGATGACAG ATCAGGTAAA CTCGGTTTGG ATGAATTCAC ATCTCTATGG 
ACCAAAGTTA GACATTGGTG TGATGTCTTC AAAAGCCATG ACAAAGATTC AAGTGGAACC 
ATTAACACTG CAGAGCTTCG TGCTGCTCTT CACGAAACTG GCCTGTCTGT TAATCGGCAA 
ATACTGATTC TCATTGTTCA TCGTTATGGA GATATTAGTG GCAAAAAACA CGATAATTCA 
GTGAAACACG AGAGACATTT ATCATTTGAT AACTTTATCC ACGTTTGCTT AAAATTGAAA 
CACAGTATTG AAATTTGGAA CAATCAAAAC TCATCTTTTG CTCGTTCGCT ACCAAAAGTC 
TCCTCAGGAG CTTTATTCAC TCTCGATGAG TGGGTTGAAA AAGTAATGTA CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC107364063 cDNA ORF clone, Tetranychus urticae(two-spotted spider mite)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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