LOC777974 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC777974
Entrez Gene ID	777974
Full Name	interleukin-12 alpha
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001078598.1	NP_001072066.1	interleukin-12 alpha precursor

Pathways from BioSystems
Gene Ontology

The following LOC777974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC777974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo17902	NM_001078598.1 Latest version!	Takifugu rubripes interleukin-12 alpha (LOC777974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC777974 ( NM_001078598.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> NP_001072066.1 Takifugu rubripes interleukin-12 alpha (LOC777974), mRNA.

Price & Availability↑

CloneID	OTo17902
Clone ID Related Accession (Same CDS sequence)	NM_001078598.1
Accession Version	NM_001078598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-16
Organism	Takifugu rubripes(torafugu)
Product	interleukin-12 alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ555461.1. ##Evidence-Data-START## Transcript exon combination :: AJ555461.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCTCGC TCCACCTCTG TGAGTATTCT GGGATGGCCG CGCTCCACCT CTACTCGTCT 
CCTGCGGCGC TGCTCCTGCT CCTCGTGTGT CCTCTGTGGC AGCTCGCTCA GGCTCTACCA 
CTGACGGGAC GGGTCTCGGA CCAGTGTGTC TCTCAGGCCC AGGCGCTCCT GCACAACATC 
ACCGGAACGC TGTCCAAGAA GGAGCTGTTC AGTGGAATGG ACTGCAGCAA GCAGAGCCTG 
GAGCTCCACG AGGAGACGCA GACGGCCTGG GCGTGCTGGC CAAAGGGACC GACGTGCTCG 
GGAACCATCA AGTCAGAATT CAACAAGGAA CGGTGTCTGA CCAACATCGC TGAGGACCTG 
CAGCACTACC TCCGGTTCCT CTCGGCCCAG CCGGACCCTG AGGGCTGGCT CCACGCCACC 
CTTCTGCCCA GCACACGGCA GCTGCTGCAG GTTGCCGTAA ACCACCCTAG CAGCTATGCT 
GAGAGGCTGA AGCTATGCAA AGTTCTGAGA GGCTTCCAGG TCCGAAGCAT CACCATCAAC 
AGAGCTGTGA AGTACATGAA CGCTGGTGCC GATGCTCAGC CCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072066.1
CDS	11..601
Translation	MASLHLCEYSGMAALHLYSSPAALLLLLVCPLWQLAQALPLTGRVSDQCVSQAQALLHNITGTLSKKELFSGMDCSKQSLELHEETQTAWACWPKGPTCSGTIKSEFNKERCLTNIAEDLQHYLRFLSAQPDPEGWLHATLLPSTRQLLQVAVNHPSSYAERLKLCKVLRGFQVRSITINRAVKYMNAGADAQPSS

Target ORF information:

RefSeq Version	NM_001078598.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes interleukin-12 alpha (LOC777974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078598.1

ORF Insert Sequence:

ATGGCCTCGC TCCACCTCTG TGAGTATTCT GGGATGGCCG CGCTCCACCT CTACTCGTCT 
CCTGCGGCGC TGCTCCTGCT CCTCGTGTGT CCTCTGTGGC AGCTCGCTCA GGCTCTACCA 
CTGACGGGAC GGGTCTCGGA CCAGTGTGTC TCTCAGGCCC AGGCGCTCCT GCACAACATC 
ACCGGAACGC TGTCCAAGAA GGAGCTGTTC AGTGGAATGG ACTGCAGCAA GCAGAGCCTG 
GAGCTCCACG AGGAGACGCA GACGGCCTGG GCGTGCTGGC CAAAGGGACC GACGTGCTCG 
GGAACCATCA AGTCAGAATT CAACAAGGAA CGGTGTCTGA CCAACATCGC TGAGGACCTG 
CAGCACTACC TCCGGTTCCT CTCGGCCCAG CCGGACCCTG AGGGCTGGCT CCACGCCACC 
CTTCTGCCCA GCACACGGCA GCTGCTGCAG GTTGCCGTAA ACCACCCTAG CAGCTATGCT 
GAGAGGCTGA AGCTATGCAA AGTTCTGAGA GGCTTCCAGG TCCGAAGCAT CACCATCAAC 
AGAGCTGTGA AGTACATGAA CGCTGGTGCC GATGCTCAGC CCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.