tnfa cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnfa
Entrez Gene ID	654303
Full Name	tumor necrosis factor alpha
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037985.1	NP_001033074.1	tumor necrosis factor alpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A novel tumor necrosis factor (TNF) gene present in tandem with theTNF-alpha gene on the same chromosome in teleosts.
Savan R, Kono T, Igawa D, Sakai M
Immunogenetics57(1-2)140-50(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo17941	NM_001037985.1 Latest version!	Takifugu rubripes tumor necrosis factor alpha (tnfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnfa ( NM_001037985.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> NP_001033074.1 Takifugu rubripes tumor necrosis factor alpha (tnfa), mRNA.

Price & Availability↑

CloneID	OTo17941
Clone ID Related Accession (Same CDS sequence)	NM_001037985.1
Accession Version	NM_001037985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Takifugu rubripes(torafugu)
Product	tumor necrosis factor alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB183465.1. ##Evidence-Data-START## Transcript exon combination :: AB183465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02369525, SAMN02384703 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAATT ACATGACAAC AGCCAGCGAC GTGGAAATGG GTCTTCAGCA GAAGACCGTG 
GTCTTAGTGG AAAAGAAATC CTCTACTGGG TGGATGGGGA AGATCATCCT GGCCATTTTC 
GTGGTGGTCC TCTGTTGCGG AGGCGCCCTG CTGTTTGTGA GCTACTGGAA CGGAAGGCAA 
GAGATGCAGG CTGTTCCAGA AAAATCTGAA ACACTAATTG AGAAGAAGGA TACAGATCCC 
CACTACACGC TGAGCCGCAT CAGCAGCAAA GCCAAGGCAG CCATCCATTT AGAAGGCAGC 
TTCGATGAAG GAGAGAACAG AAAAGACCAG GTGGAGTGGA AGAACGGTCA AGGCCAGGCG 
TTCGCTCAGG GCGACTTCCA GCTCGACAAC AACACCATCA TCATTCCCAA AACCGGCCTG 
TACTTTGTTT ACAGCCAGGC TTCTTTCCGA GTGACTTGCG GCGAAGGGGA CAAACACTCG 
CCGGGAAAAA GCCACATTCC CCTCAGCCAC AGGGTTTGGC GGTACTCGGA CTCCATCGGC 
ACTGAGACCA CTCTGCTGAA CGCGGTGAGG TCAGCGTGTC AGAACTCGGC GCTGGAGGGA 
GGCTACAGCG AGGGACAAAG CTGCTACAAC GCCATTTACC TGGGCGCCGT CTTCCAGCTG 
AAAATGGGGG ACAAACTTCG GACCGAGACC AACCAGCTGT CCGAGCTGGA GACGGAGGAG 
GGCAAGACTT TCTTCGGTGT GTTTGCACTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033074.1
CDS	27..779
Translation	MVNYMTTASDVEMGLQQKTVVLVEKKSSTGWMGKIILAIFVVVLCCGGALLFVSYWNGRQEMQAVPEKSETLIEKKDTDPHYTLSRISSKAKAAIHLEGSFDEGENRKDQVEWKNGQGQAFAQGDFQLDNNTIIIPKTGLYFVYSQASFRVTCGEGDKHSPGKSHIPLSHRVWRYSDSIGTETTLLNAVRSACQNSALEGGYSEGQSCYNAIYLGAVFQLKMGDKLRTETNQLSELETEEGKTFFGVFAL

Target ORF information:

RefSeq Version	NM_001037985.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tumor necrosis factor alpha (tnfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037985.1

ORF Insert Sequence:

ATGGTGAATT ACATGACAAC AGCCAGCGAC GTGGAAATGG GTCTTCAGCA GAAGACCGTG 
GTCTTAGTGG AAAAGAAATC CTCTACTGGG TGGATGGGGA AGATCATCCT GGCCATTTTC 
GTGGTGGTCC TCTGTTGCGG AGGCGCCCTG CTGTTTGTGA GCTACTGGAA CGGAAGGCAA 
GAGATGCAGG CTGTTCCAGA AAAATCTGAA ACACTAATTG AGAAGAAGGA TACAGATCCC 
CACTACACGC TGAGCCGCAT CAGCAGCAAA GCCAAGGCAG CCATCCATTT AGAAGGCAGC 
TTCGATGAAG GAGAGAACAG AAAAGACCAG GTGGAGTGGA AGAACGGTCA AGGCCAGGCG 
TTCGCTCAGG GCGACTTCCA GCTCGACAAC AACACCATCA TCATTCCCAA AACCGGCCTG 
TACTTTGTTT ACAGCCAGGC TTCTTTCCGA GTGACTTGCG GCGAAGGGGA CAAACACTCG 
CCGGGAAAAA GCCACATTCC CCTCAGCCAC AGGGTTTGGC GGTACTCGGA CTCCATCGGC 
ACTGAGACCA CTCTGCTGAA CGCGGTGAGG TCAGCGTGTC AGAACTCGGC GCTGGAGGGA 
GGCTACAGCG AGGGACAAAG CTGCTACAAC GCCATTTACC TGGGCGCCGT CTTCCAGCTG 
AAAATGGGGG ACAAACTTCG GACCGAGACC AACCAGCTGT CCGAGCTGGA GACGGAGGAG 
GGCAAGACTT TCTTCGGTGT GTTTGCACTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel tumor necrosis factor (TNF) gene present in tandem with theTNF-alpha gene on the same chromosome in teleosts.
Immunogenetics57(1-2)140-50(2005 Apr)
Savan R,Kono T,Igawa D,Sakai M