st3gal2 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	st3gal2
Entrez Gene ID	445972
Full Name	ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Synonyms	siat5
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011620457.1	XP_011618759.1	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2
XM_029832072.1	XP_029687932.1	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R
Glycobiology15(8)805-17(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The characterization of the only sialyltransferase gene found in the tunicate Ciona intestinalis and ST3Gal II gene from the bony fish Takifugu rubripes, is reported.
Title: The evolution of galactose alpha2,3-sialyltransferase: Ciona intestinalis ST3GAL I/II and Takifugu rubripes ST3GAL II sialylate Galbeta1,3GalNAc structures on glycoproteins but not glycolipids.

The following st3gal2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the st3gal2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo18325	XM_011620457.1 Latest version!	Takifugu rubripes ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (st3gal2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

st3gal2 ( XM_011620457.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011618759.1 Takifugu rubripes ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (st3gal2), mRNA.

Price & Availability↑

CloneID	OTo18325
Clone ID Related Accession (Same CDS sequence)	XM_011620457.1
Accession Version	XM_011620457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004073025.1) and transcript sequence (AJ626817.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases assembly gap :: added 60 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCGTGG GGGGGCCGCT GTCTGAGCTC AGGGACTGGC CCCCCGGGGG GACGCCCGGT 
CCAGGGGGGG ACGGCGTTGA TAAGGGGAGC AAGGCCAGGT GCTCCTGCAG GGTCTGGGTG 
TTCCTGGTCT CGCTGACCCT GGTCTTCCTC ACCTCGCTCT TCTTCTCCGT CTCGCTGCGC 
GGGGGCGTTG GGTTCAACTA CCTGGAGGCG CCGGGGTGGG AGGAGTCGAG GCGCGTGAAG 
CTGGTGCCCA GCTACGCAGG TGCACGCCGC CCGTCTGCAG CCGCCAGCAG CCAGCAGAGG 
ACCTGCGAGT GTCCTCGCTG CGTGGGCGAC CCCGGCGTCT CCGACTGGTT CGACGAGAAC 
TACGACCCCG ACGTGTCTCC GGTCTGGACC AGGGACAACG GCCAGCTGCC TCCGGACGTC 
TACTACTGGT GGGTGATGCT GCAGCCCCAG TTCAAGCCCC ACAGCATCCA GCAGGTCCTG 
CTCAAGCTCT TCCAGGTGGT CCCAGGAAGG TCGCCCTACG GCTCCTGGGA CCCGGGACGC 
TGTCTGCGCT GTGCTGTTGT GGGCAACTCT GGGAACCTGC GGGGGGCCGG ATACGGAGCC 
ACCATCGATG GGCACAACTA CATCATGAGG ATCAACTTGG CTCCGACCGT GGGCTTCGAA 
GAGGACGCGG GCAGCCACAC CACTCATCAC TTCATGTACC CGGAGAGCGC CAAGAACCTG 
GCGGCCAACG TCAGCTTCGT TCTGGTCCCC TTCAAGACGC TGGACCTGGT GTGGATCACC 
AGCGCTCTGT CCACGGGTCA GATCCGATTC ACCTACGCTC CGGTCAAGCA GTTCCTGCGG 
GTGGACAAGG ACAAGGTCCA GATCTTCAAC CCTGCGTTCT TCAAATACGT GCACGACCGC 
TGGACCAGAC ACCACGGGCG CTACCCGTCC ACCGGCATGC TGGTGCTGTT CTTCGCCCTG 
CACGTCTGCG ATGAGGTCAA TGTCTTCGGC TTTGGGGCGG ACGGCCGTGG GAACTGGCAC 
CACTACTGGG AGCAGAACCG CTACTCTGGA GAATTCCGGA AGACCGGCGT CCACGATGCG 
GACTACGAAG CCCAGATCAT CCAGCGGCTG GCCAAGGCCG GCAAGATCAC CGTGTTCCCT 
GGGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011618759.1
CDS	1..1149
Translation	MAVGGPLSELRDWPPGGTPGPGGDGVDKGSKARCSCRVWVFLVSLTLVFLTSLFFSVSLRGGVGFNYLEAPGWEESRRVKLVPSYAGARRPSAAASSQQRTCECPRCVGDPGVSDWFDENYDPDVSPVWTRDNGQLPPDVYYWWVMLQPQFKPHSIQQVLLKLFQVVPGRSPYGSWDPGRCLRCAVVGNSGNLRGAGYGATIDGHNYIMRINLAPTVGFEEDAGSHTTHHFMYPESAKNLAANVSFVLVPFKTLDLVWITSALSTGQIRFTYAPVKQFLRVDKDKVQIFNPAFFKYVHDRWTRHHGRYPSTGMLVLFFALHVCDEVNVFGFGADGRGNWHHYWEQNRYSGEFRKTGVHDADYEAQIIQRLAKAGKITVFPGK

Target ORF information:

RefSeq Version	XM_011620457.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (st3gal2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011620457.1

ORF Insert Sequence:

ATGGCCGTGG GGGGGCCGCT GTCTGAGCTC AGGGACTGGC CCCCCGGGGG GACGCCCGGT 
CCAGGGGGGG ACGGCGTTGA TAAGGGGAGC AAGGCCAGGT GCTCCTGCAG GGTCTGGGTG 
TTCCTGGTCT CGCTGACCCT GGTCTTCCTC ACCTCGCTCT TCTTCTCCGT CTCGCTGCGC 
GGGGGCGTTG GGTTCAACTA CCTGGAGGCG CCGGGGTGGG AGGAGTCGAG GCGCGTGAAG 
CTGGTGCCCA GCTACGCAGG TGCACGCCGC CCGTCTGCAG CCGCCAGCAG CCAGCAGAGG 
ACCTGCGAGT GTCCTCGCTG CGTGGGCGAC CCCGGCGTCT CCGACTGGTT CGACGAGAAC 
TACGACCCCG ACGTGTCTCC GGTCTGGACC AGGGACAACG GCCAGCTGCC TCCGGACGTC 
TACTACTGGT GGGTGATGCT GCAGCCCCAG TTCAAGCCCC ACAGCATCCA GCAGGTCCTG 
CTCAAGCTCT TCCAGGTGGT CCCAGGAAGG TCGCCCTACG GCTCCTGGGA CCCGGGACGC 
TGTCTGCGCT GTGCTGTTGT GGGCAACTCT GGGAACCTGC GGGGGGCCGG ATACGGAGCC 
ACCATCGATG GGCACAACTA CATCATGAGG ATCAACTTGG CTCCGACCGT GGGCTTCGAA 
GAGGACGCGG GCAGCCACAC CACTCATCAC TTCATGTACC CGGAGAGCGC CAAGAACCTG 
GCGGCCAACG TCAGCTTCGT TCTGGTCCCC TTCAAGACGC TGGACCTGGT GTGGATCACC 
AGCGCTCTGT CCACGGGTCA GATCCGATTC ACCTACGCTC CGGTCAAGCA GTTCCTGCGG 
GTGGACAAGG ACAAGGTCCA GATCTTCAAC CCTGCGTTCT TCAAATACGT GCACGACCGC 
TGGACCAGAC ACCACGGGCG CTACCCGTCC ACCGGCATGC TGGTGCTGTT CTTCGCCCTG 
CACGTCTGCG ATGAGGTCAA TGTCTTCGGC TTTGGGGCGG ACGGCCGTGG GAACTGGCAC 
CACTACTGGG AGCAGAACCG CTACTCTGGA GAATTCCGGA AGACCGGCGT CCACGATGCG 
GACTACGAAG CCCAGATCAT CCAGCGGCTG GCCAAGGCCG GCAAGATCAC CGTGTTCCCT 
GGGAAGTAA