prim1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	prim1
Entrez Gene ID	101077321
Full Name	DNA primase subunit 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011602630.1	XP_011600932.1	DNA primase small subunit
XM_011602630.2	XP_011600932.2	DNA primase small subunit

Pathways from BioSystems
Gene Ontology

The following prim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo30305	XM_011602630.1 Latest version!	Takifugu rubripes primase, DNA, polypeptide 1 (49kDa) (prim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OTo63873	XM_011602630.2 Latest version!	Takifugu rubripes DNA primase subunit 1 (prim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prim1 ( XM_011602630.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011600932.1 Takifugu rubripes primase, DNA, polypeptide 1 (49kDa) (prim1), mRNA.

Price & Availability↑

CloneID	OTo30305
Clone ID Related Accession (Same CDS sequence)	XM_011602630.1
Accession Version	XM_011602630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DNA primase small subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070935.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCAA ATGTATTTGA CTCGGCAAAT TTGCCGGACC TTTTGCCGCT GTACTACCGA 
CGGCTGTTCC CCTTTTCTCT GTATTACCGG TGGCTTACTT ATGGAGGACT GCAGAAGAAC 
TACTTTCAGA ATCGCGAGTT CTCCTTCACC CTCAAGGATG ACATCTACGT GCGTTACCAG 
TCATTCAGCA CACAGACCGA GCTGGAAAAA GAAATGCAAA AGATAAACCC ATACAAGGTT 
GACATTGGAG CAGTCTACAG TCACAGGCCT ATCCAGCACA ACACTGTGAA ATCAGGAACC 
TTCCAGGCCC TGGAGAAGGA GCTGGTGTTT GATATCGATA TGACAGATTA TGATGATGTA 
AGAAGCTGCT GCAGTGCTGC AGATATCTGC TCCAAATGCT GGACACTGAT GACCATTGCC 
ATTCGAATCC TGGACAGAGC TCTCAGAGAG GATTTTGGAT TCCAACACTT GCTTTGGGTT 
TATTCTGGTA GAAGAGGAGT ACATTGCTGG GTGTGTGATG AGGCTGCCAG GAAGCTCTCT 
GTGGCAGCGC GCTCTGCTGT GGCAGAATAC CTCAGCCTAG TCAAGGGAGG TGACGAGACG 
GTAAAGAAAG TTGTTCTGAC AGATCCAATT CATCCTTTCA TTCGTGAATC ACTGGCAGTG 
GTGGAGAAAT ACTTTACCCA GTATGCAATA CATGACCAGG ACATACTCGG ACAGAAAGAA 
TCTGTTGAAA AGGTTTTGGC ACTGGTGCCC GAAGATGTGA GGAAAGAATT ACAGCAGGAT 
TTTCAAAATG AAAGGAAACC CGAGAACCGC TGGACACAGA TCCGAAATCA AGCCATCAAG 
AAACAGGGCG CTGCGAAAAA GGGCCAACAT TTTGAGAAGG AAATCATGCT GCAGTATTGT 
TACCCACGAC TTGACGTGAA CGTCAGTAAG GGGGTCAACC ACTTGCTGAA GAGCCCCTTC 
AGTGTTCACC CCAAAACAGG GCGCATTTCT GTCCCCATTG ATTTGGAAGA ATTAGATTCT 
TTTGATCCGT TTGCAGTGCC CACGATCAGT TTGATTTGTG AAGAGCTTGA TCGACCAAGG 
AGAGGTGAAG AAGATGAAAA GTTAGATGAT ACCAAGGAGA ATGAGAAAGA TGCCGCAGAA 
AGACGAAAGA TCAGAGATTA CAAAAGGACA AGCCTGGCAA AGTACGTGAA ACATTTTGAT 
CAGTTTCTGG CTCAAATGGC TCAGTCGTGG AAAGGAGATC TTCTCAAAAA AAGCGATCTC 
CAGAAACAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600932.1
CDS	116..1390
Translation	MPSNVFDSANLPDLLPLYYRRLFPFSLYYRWLTYGGLQKNYFQNREFSFTLKDDIYVRYQSFSTQTELEKEMQKINPYKVDIGAVYSHRPIQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICSKCWTLMTIAIRILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLVKGGDETVKKVVLTDPIHPFIRESLAVVEKYFTQYAIHDQDILGQKESVEKVLALVPEDVRKELQQDFQNERKPENRWTQIRNQAIKKQGAAKKGQHFEKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLEELDSFDPFAVPTISLICEELDRPRRGEEDEKLDDTKENEKDAAERRKIRDYKRTSLAKYVKHFDQFLAQMAQSWKGDLLKKSDLQKQF

Target ORF information:

RefSeq Version	XM_011602630.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes primase, DNA, polypeptide 1 (49kDa) (prim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602630.1

ORF Insert Sequence:

ATGCCTTCAA ATGTATTTGA CTCGGCAAAT TTGCCGGACC TTTTGCCGCT GTACTACCGA 
CGGCTGTTCC CCTTTTCTCT GTATTACCGG TGGCTTACTT ATGGAGGACT GCAGAAGAAC 
TACTTTCAGA ATCGCGAGTT CTCCTTCACC CTCAAGGATG ACATCTACGT GCGTTACCAG 
TCATTCAGCA CACAGACCGA GCTGGAAAAA GAAATGCAAA AGATAAACCC ATACAAGGTT 
GACATTGGAG CAGTCTACAG TCACAGGCCT ATCCAGCACA ACACTGTGAA ATCAGGAACC 
TTCCAGGCCC TGGAGAAGGA GCTGGTGTTT GATATCGATA TGACAGATTA TGATGATGTA 
AGAAGCTGCT GCAGTGCTGC AGATATCTGC TCCAAATGCT GGACACTGAT GACCATTGCC 
ATTCGAATCC TGGACAGAGC TCTCAGAGAG GATTTTGGAT TCCAACACTT GCTTTGGGTT 
TATTCTGGTA GAAGAGGAGT ACATTGCTGG GTGTGTGATG AGGCTGCCAG GAAGCTCTCT 
GTGGCAGCGC GCTCTGCTGT GGCAGAATAC CTCAGCCTAG TCAAGGGAGG TGACGAGACG 
GTAAAGAAAG TTGTTCTGAC AGATCCAATT CATCCTTTCA TTCGTGAATC ACTGGCAGTG 
GTGGAGAAAT ACTTTACCCA GTATGCAATA CATGACCAGG ACATACTCGG ACAGAAAGAA 
TCTGTTGAAA AGGTTTTGGC ACTGGTGCCC GAAGATGTGA GGAAAGAATT ACAGCAGGAT 
TTTCAAAATG AAAGGAAACC CGAGAACCGC TGGACACAGA TCCGAAATCA AGCCATCAAG 
AAACAGGGCG CTGCGAAAAA GGGCCAACAT TTTGAGAAGG AAATCATGCT GCAGTATTGT 
TACCCACGAC TTGACGTGAA CGTCAGTAAG GGGGTCAACC ACTTGCTGAA GAGCCCCTTC 
AGTGTTCACC CCAAAACAGG GCGCATTTCT GTCCCCATTG ATTTGGAAGA ATTAGATTCT 
TTTGATCCGT TTGCAGTGCC CACGATCAGT TTGATTTGTG AAGAGCTTGA TCGACCAAGG 
AGAGGTGAAG AAGATGAAAA GTTAGATGAT ACCAAGGAGA ATGAGAAAGA TGCCGCAGAA 
AGACGAAAGA TCAGAGATTA CAAAAGGACA AGCCTGGCAA AGTACGTGAA ACATTTTGAT 
CAGTTTCTGG CTCAAATGGC TCAGTCGTGG AAAGGAGATC TTCTCAAAAA AAGCGATCTC 
CAGAAACAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prim1 ( XM_011602630.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011600932.2 Takifugu rubripes DNA primase subunit 1 (prim1), mRNA.

Price & Availability↑

CloneID	OTo63873
Clone ID Related Accession (Same CDS sequence)	XM_011602630.2
Accession Version	XM_011602630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	DNA primase small subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCAA ATGTATTTGA CTCGGCAAAT TTGCCGGACC TTTTGCCGCT GTACTACCGA 
CGGCTGTTCC CCTTTTCTCT GTATTACCGG TGGCTTACTT ATGGAGGACT GCAGAAGAAC 
TACTTTCAGA ATCGCGAGTT CTCCTTCACC CTCAAGGATG ACATCTACGT GCGTTACCAG 
TCATTCAGCA CACAGACCGA GCTGGAAAAA GAAATGCAAA AGATAAACCC ATACAAGATT 
GACATTGGAG CAGTCTACAG TCACAGGCCT ATCCAGCACA ACACTGTGAA ATCAGGAACC 
TTCCAGGCCC TGGAGAAGGA GCTGGTGTTT GATATCGATA TGACAGATTA TGATGATGTA 
AGAAGCTGCT GCAGTGCTGC AGATATCTGC TGCAAATGCT GGACACTGAT GACCATTGCC 
ATTCGAATCC TGGACAGAGC TCTCAGAGAG GATTTTGGAT TCCAACACTT GCTTTGGGTT 
TATTCTGGTA GAAGAGGAGT ACATTGCTGG GTGTGTGATG AGGCTGCCAG GAAGCTCTCT 
GTGGCAGCGC GCTCTGCTGT GGCAGAATAC CTCAGCCTAG TCAAGGGAGG TGACGAGACG 
GTAAAGAAAG TTGTTCTGAC AGATCCAATT CATCCTTTCA TTCGTGAATC ACTGGCAGTG 
GTGGAGAAAT ACTTTACCCA GTATGCAATA CATGACCAGG ACATACTCGG ACAGAAAGAA 
TCTGTTGAAA AGGTTTTGGC ACTGGTGCCC GAAGATGTGA GGAAAGAATT ACAGCAGGAT 
TTTCAAAATG AAAGGAAACC CGAGAACCGC TGGACACAGA TCCGAAATCA AGCCATCAAG 
AAACAGGGCG CTGCGAAAAA GGGCCAACAT TTTGAGAAGG AAATCATGCT GCAGTATTGT 
TACCCACGAC TTGACGTGAA CGTCAGTAAG GGGGTCAACC ACTTGCTGAA GAGCCCCTTC 
AGTGTTCACC CCAAAACAGG GCGCATTTCT GTCCCCATTG ATTTGGAAGA ATTAGATTCT 
TTTGATCCGT TTGCAGTGCC CACGATCAGT TTGATTTGTG AAGAGCTTGA TCGACCAAGG 
AGAGGTGAAG AAGATGAAAA GTTAGATGAT ACCAAGGAGA ATGAGAAAGA TGCCGCAGAA 
AGACGAAAGA TCAGAGATTA CAAAAGGACA AGCCTGGCAA AGTACGTGAA ACATTTTGAT 
CAGTTTCTGG CTCAAATGGC TCAGTCGTGG AAAGGAGATC TTCTCAAAAA AAGCGATCTC 
CAGAAACAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600932.2
CDS	136..1410
Translation	MPSNVFDSANLPDLLPLYYRRLFPFSLYYRWLTYGGLQKNYFQNREFSFTLKDDIYVRYQSFSTQTELEKEMQKINPYKIDIGAVYSHRPIQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICCKCWTLMTIAIRILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLVKGGDETVKKVVLTDPIHPFIRESLAVVEKYFTQYAIHDQDILGQKESVEKVLALVPEDVRKELQQDFQNERKPENRWTQIRNQAIKKQGAAKKGQHFEKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLEELDSFDPFAVPTISLICEELDRPRRGEEDEKLDDTKENEKDAAERRKIRDYKRTSLAKYVKHFDQFLAQMAQSWKGDLLKKSDLQKQF

Target ORF information:

RefSeq Version	XM_011602630.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DNA primase subunit 1 (prim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602630.2

ORF Insert Sequence:

ATGCCTTCAA ATGTATTTGA CTCGGCAAAT TTGCCGGACC TTTTGCCGCT GTACTACCGA 
CGGCTGTTCC CCTTTTCTCT GTATTACCGG TGGCTTACTT ATGGAGGACT GCAGAAGAAC 
TACTTTCAGA ATCGCGAGTT CTCCTTCACC CTCAAGGATG ACATCTACGT GCGTTACCAG 
TCATTCAGCA CACAGACCGA GCTGGAAAAA GAAATGCAAA AGATAAACCC ATACAAGATT 
GACATTGGAG CAGTCTACAG TCACAGGCCT ATCCAGCACA ACACTGTGAA ATCAGGAACC 
TTCCAGGCCC TGGAGAAGGA GCTGGTGTTT GATATCGATA TGACAGATTA TGATGATGTA 
AGAAGCTGCT GCAGTGCTGC AGATATCTGC TGCAAATGCT GGACACTGAT GACCATTGCC 
ATTCGAATCC TGGACAGAGC TCTCAGAGAG GATTTTGGAT TCCAACACTT GCTTTGGGTT 
TATTCTGGTA GAAGAGGAGT ACATTGCTGG GTGTGTGATG AGGCTGCCAG GAAGCTCTCT 
GTGGCAGCGC GCTCTGCTGT GGCAGAATAC CTCAGCCTAG TCAAGGGAGG TGACGAGACG 
GTAAAGAAAG TTGTTCTGAC AGATCCAATT CATCCTTTCA TTCGTGAATC ACTGGCAGTG 
GTGGAGAAAT ACTTTACCCA GTATGCAATA CATGACCAGG ACATACTCGG ACAGAAAGAA 
TCTGTTGAAA AGGTTTTGGC ACTGGTGCCC GAAGATGTGA GGAAAGAATT ACAGCAGGAT 
TTTCAAAATG AAAGGAAACC CGAGAACCGC TGGACACAGA TCCGAAATCA AGCCATCAAG 
AAACAGGGCG CTGCGAAAAA GGGCCAACAT TTTGAGAAGG AAATCATGCT GCAGTATTGT 
TACCCACGAC TTGACGTGAA CGTCAGTAAG GGGGTCAACC ACTTGCTGAA GAGCCCCTTC 
AGTGTTCACC CCAAAACAGG GCGCATTTCT GTCCCCATTG ATTTGGAAGA ATTAGATTCT 
TTTGATCCGT TTGCAGTGCC CACGATCAGT TTGATTTGTG AAGAGCTTGA TCGACCAAGG 
AGAGGTGAAG AAGATGAAAA GTTAGATGAT ACCAAGGAGA ATGAGAAAGA TGCCGCAGAA 
AGACGAAAGA TCAGAGATTA CAAAAGGACA AGCCTGGCAA AGTACGTGAA ACATTTTGAT 
CAGTTTCTGG CTCAAATGGC TCAGTCGTGG AAAGGAGATC TTCTCAAAAA AAGCGATCTC 
CAGAAACAAT TTTAA