twsg1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	twsg1
Entrez Gene ID	101065019
Full Name	twisted gastrulation BMP signaling modulator 1
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003975346.3	XP_003975395.2	twisted gastrulation protein homolog 1
XM_003975346.2	XP_003975395.1	twisted gastrulation protein homolog 1

Pathways from BioSystems
Gene Ontology

The following twsg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twsg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo14200	XM_003975346.2 Latest version!	Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OTo62976	XM_003975346.3 Latest version!	Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twsg1 ( XM_003975346.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003975395.1 Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Price & Availability↑

CloneID	OTo14200
Clone ID Related Accession (Same CDS sequence)	XM_003975346.2
Accession Version	XM_003975346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	twisted gastrulation protein homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071160.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003975346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCG GTCAGACGAC CCTCCCCGCC GCCGCCGCCG CCACTCTCCT CTTCCTCCTG 
TCGGGGCTGG CTCTGACCTC CGGCTGCAAC AAGGCGCTCT GTGCCAGCGA CGTCAGCAAG 
TGTCTCATCC AGGAGCTGTG CCAGTGTCGT CCATCTGATG GGAACTGTTC CTGCTGTAAA 
GAGTGCATGT TGTGTCTGGG GAACCTTTGG GAGGAGTGCT GCGACTGCGT CGGGATGTGT 
AATCCCAAGA ACTACAGTGA CTCTCCAGCA ACATCCAGGA GCACCGTGGA GGAGCTCCAC 
CGCCCCATCC CCTCCTTGTT CCGCGCGCTC ACCGAAGGCG AGTCACCGAT CAACATGATG 
GTGGTGTCGT TCCCCATCGC GGAGGAGCTG TCTCACCACG AGAACTTGGT GTCCTTCCTG 
GAGTCGCTAG ACAACCCGCA CCAGAATGTT TCTGTGCCTG GAAACAGCAT CCACACCAGC 
TATGACGCTC AAGAAAACAT GTGCACGGTG GTCTACTTTG ATGAATGCGT GTCCATCCGC 
CAGTGCAAAA TGTACTGCGA GTCAATGGGA GGATCCAAGT ACCGCTGGTT TCACAATGCC 
TGCTGCCAAT GCATCGGACC CGAATGCGTG GACTATGGCA GCAAGGCCGT AAAGTGCATG 
AACTGTCTGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975395.1
CDS	1127..1801
Translation	MRPGQTTLPAAAAATLLFLLSGLALTSGCNKALCASDVSKCLIQELCQCRPSDGNCSCCKECMLCLGNLWEECCDCVGMCNPKNYSDSPATSRSTVEELHRPIPSLFRALTEGESPINMMVVSFPIAEELSHHENLVSFLESLDNPHQNVSVPGNSIHTSYDAQENMCTVVYFDECVSIRQCKMYCESMGGSKYRWFHNACCQCIGPECVDYGSKAVKCMNCLL

Target ORF information:

RefSeq Version	XM_003975346.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975346.2

ORF Insert Sequence:

ATGAGGCCCG GTCAGACGAC CCTCCCCGCC GCCGCCGCCG CCACTCTCCT CTTCCTCCTG 
TCGGGGCTGG CTCTGACCTC CGGCTGCAAC AAGGCGCTCT GTGCCAGCGA CGTCAGCAAG 
TGTCTCATCC AGGAGCTGTG CCAGTGTCGT CCATCTGATG GGAACTGTTC CTGCTGTAAA 
GAGTGCATGT TGTGTCTGGG GAACCTTTGG GAGGAGTGCT GCGACTGCGT CGGGATGTGT 
AATCCCAAGA ACTACAGTGA CTCTCCAGCA ACATCCAGGA GCACCGTGGA GGAGCTCCAC 
CGCCCCATCC CCTCCTTGTT CCGCGCGCTC ACCGAAGGCG AGTCACCGAT CAACATGATG 
GTGGTGTCGT TCCCCATCGC GGAGGAGCTG TCTCACCACG AGAACTTGGT GTCCTTCCTG 
GAGTCGCTAG ACAACCCGCA CCAGAATGTT TCTGTGCCTG GAAACAGCAT CCACACCAGC 
TATGACGCTC AAGAAAACAT GTGCACGGTG GTCTACTTTG ATGAATGCGT GTCCATCCGC 
CAGTGCAAAA TGTACTGCGA GTCAATGGGA GGATCCAAGT ACCGCTGGTT TCACAATGCC 
TGCTGCCAAT GCATCGGACC CGAATGCGTG GACTATGGCA GCAAGGCCGT AAAGTGCATG 
AACTGTCTGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twsg1 ( XM_003975346.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003975395.2 Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Price & Availability↑

CloneID	OTo62976
Clone ID Related Accession (Same CDS sequence)	XM_003975346.3
Accession Version	XM_003975346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	twisted gastrulation protein homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003975346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCG GTCAGACGAC CCTCCCCGCC GCCGCCGCCA CTCTCCTCTT CCTCCTGTCG 
GGGCTGGCTC TGACCTCCGG CTGCAACAAG GCGCTCTGTG CCAGCGACGT CAGCAAGTGT 
CTCATCCAGG AGCTGTGCCA GTGTCGTCCA TCTGATGGGA ACTGTTCCTG CTGTAAAGAG 
TGCATGTTGT GTCTGGGGAA CCTTTGGGAG GAGTGCTGCG ACTGCGTCGG GATGTGTAAT 
CCCAAGAACT ACAGTGACTC TCCAGCAACA TCCAGGAGCA CCGTGGAGGA GCTCCACCGC 
CCCATCCCCT CCTTGTTCCG CGCGCTCACC GAAGGCGAGT CACCGATCAA CATGATGGTG 
GTGTCGTTCC CCATCGCGGA GGAGCTGTCT CACCACGAGA ACTTGGTGTC CTTCCTGGAG 
TCGCTAGACA ACCCGCACCA GAATGTTTCT GTGCCTGGAA ACAGCATCCA CACCAGCTAT 
GACGCTCAAG AAAACATGTG CACGGTGGTC TACTTTGATG AATGCGTGTC CATCCGCCAG 
TGCAAAATGT ACTGCGAGTC AATGGGAGGA TCCAAGTACC GCTGGTTTCA CAATGCCTGC 
TGCCAATGCA TCGGACCCGA ATGCGTGGAC TATGGCAGCA AGGCCGTAAA GTGCATGAAC 
TGTCTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975395.2
CDS	629..1300
Translation	MRPGQTTLPAAAATLLFLLSGLALTSGCNKALCASDVSKCLIQELCQCRPSDGNCSCCKECMLCLGNLWEECCDCVGMCNPKNYSDSPATSRSTVEELHRPIPSLFRALTEGESPINMMVVSFPIAEELSHHENLVSFLESLDNPHQNVSVPGNSIHTSYDAQENMCTVVYFDECVSIRQCKMYCESMGGSKYRWFHNACCQCIGPECVDYGSKAVKCMNCLL

Target ORF information:

RefSeq Version	XM_003975346.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975346.3

ORF Insert Sequence:

ATGAGGCCCG GTCAGACGAC CCTCCCCGCC GCCGCCGCCA CTCTCCTCTT CCTCCTGTCG 
GGGCTGGCTC TGACCTCCGG CTGCAACAAG GCGCTCTGTG CCAGCGACGT CAGCAAGTGT 
CTCATCCAGG AGCTGTGCCA GTGTCGTCCA TCTGATGGGA ACTGTTCCTG CTGTAAAGAG 
TGCATGTTGT GTCTGGGGAA CCTTTGGGAG GAGTGCTGCG ACTGCGTCGG GATGTGTAAT 
CCCAAGAACT ACAGTGACTC TCCAGCAACA TCCAGGAGCA CCGTGGAGGA GCTCCACCGC 
CCCATCCCCT CCTTGTTCCG CGCGCTCACC GAAGGCGAGT CACCGATCAA CATGATGGTG 
GTGTCGTTCC CCATCGCGGA GGAGCTGTCT CACCACGAGA ACTTGGTGTC CTTCCTGGAG 
TCGCTAGACA ACCCGCACCA GAATGTTTCT GTGCCTGGAA ACAGCATCCA CACCAGCTAT 
GACGCTCAAG AAAACATGTG CACGGTGGTC TACTTTGATG AATGCGTGTC CATCCGCCAG 
TGCAAAATGT ACTGCGAGTC AATGGGAGGA TCCAAGTACC GCTGGTTTCA CAATGCCTGC 
TGCCAATGCA TCGGACCCGA ATGCGTGGAC TATGGCAGCA AGGCCGTAAA GTGCATGAAC 
TGTCTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.