LOC101062830 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101062830
Entrez Gene ID	101062830
Full Name	sphingosine kinase 1-like
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003964526.3	XP_003964575.2	sphingosine kinase 1 isoform X1
XM_029835917.1	XP_029691777.1	sphingosine kinase 1 isoform X2
XM_003964526.2	XP_003964575.1	sphingosine kinase 1-like isoform X1
XM_011603832.2	XP_011602134.2	sphingosine kinase 1 isoform X3
XM_011603832.1	XP_011602134.1	sphingosine kinase 1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101062830 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101062830 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo19908	XM_011603832.1 Latest version!	Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OTo66056	XM_011603832.2 Latest version!	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OTo03532	XM_003964526.2 Latest version!	Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo66054	XM_003964526.3 Latest version!	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo66055	XM_029835917.1 Latest version!	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101062830 ( XM_011603832.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011602134.1 Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo19908
Clone ID Related Accession (Same CDS sequence)	XM_011603832.1
Accession Version	XM_011603832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	sphingosine kinase 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070955.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG AAAATTCCTT CCACTGTGGA ATCGGAAGCT GGGGTGTGGT GTACAGCGAG 
GTGCGTCGGC CCTGCAGGGT CATGATCCTG GTCAACCCTC ACAGCGGCCG CGGCCAGGCC 
CTCCAGCTCT TCAGCGGCCA CGTCCAGGGG ATGCTCACCG AGGCCGCCGT GCCCTACAAG 
CTCGTCATCA CAGAACACCA GAACCACGCA CGGGAGCTGG TGAGGAAGGC CGACCTGTCG 
CAGTGGGACG CTCTGGTCAT CATGTCGGGA GACGGGCTGC TGTTCGAGGT GATCAATGGT 
CTGATGGACA GGGAGGACTG GGAGCAGGCC ATCCAGACCC CTCTGGGCAT CCTGCCGGGG 
GGCTCAGGTA ACGCCCTGGC TGCCTCCGTC CACCACTATT CACAGTCTCC TCCGGCGTGG 
AACGAAGAGC TGCTCCTCAG CTGCGGGTTC ATGCTGTGCA AAGGTCTGGT GGGGCCCCTG 
GACCTGGTCT CCATCCACCT GGCCTCCCAG CAGCGCCTCT TCTCCTTCCT GTCCCTGGCC 
TGGGGCTTCG TGGCCGACGT GGACATCGAG AGCGAGAAGT ACCGTCACGT GGGGGCCATC 
CGCTTCCTGA TGGGCACGCT GGTGCGCCTG GCCACCCTCC GAGTGTACCA GGGCCGGCTG 
GCGTACCTGC CCGTGAAGGA GGCGCCGAGA TACCCCAAAG GGAACGCCAA GGCCGTCCAC 
CCCCCCGCCG CGCCCCAGCG CCCCTCCCTC TGCTCTTCCC TCCCGTGCCA GCAGCTCCCG 
AACGCCTCTC CCAACCAGAA CTCCCACCGC AATCACACGA GCGCCAACTC CAACCACAAC 
AGCATCGCCA ACTCCTCCAA CAACGCCATC ACCACGAAAA GAGCCGAAGC CCAAAGCGGC 
GGCAGATCCG GAGCGCCGCC GGACTCCCTC CTCGCCGGCC TGGACCAGCC GGTCCCGGAG 
GGCTGGACGG TGGTGCGGGA GGAAGACTTT GTGCTGGTCC TGGCCATTTA CCAGTCGCAC 
CTCGCTGAGG ACCTGTGGAC GGCCCCCGGC GCCAGCGCGG ACGACGGCTT CATCCACCTG 
TTCTACGTGA CGGCGGGCAT CTCCCGTCCC GCCCTCCTGC GGCTGTTCCT CGCCATGGAG 
AAGGGCGCCC ACTTGGCGTG CGGCTGCCCT CACCTGGTGT ACGAGAAGGT GAGGGCGCTG 
CGGCTGGAGC CCATCTCCCC GCAGGGCATG ATCACCGTGG ACGGGGAGAT GGTGGAGTAC 
GGGCCGGTGG AGGCCCAGAT CCACCCGGGA CTCGCCAGGC TCGTGTGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011602134.1
CDS	365..1678
Translation	MTLENSFHCGIGSWGVVYSEVRRPCRVMILVNPHSGRGQALQLFSGHVQGMLTEAAVPYKLVITEHQNHARELVRKADLSQWDALVIMSGDGLLFEVINGLMDREDWEQAIQTPLGILPGGSGNALAASVHHYSQSPPAWNEELLLSCGFMLCKGLVGPLDLVSIHLASQQRLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLATLRVYQGRLAYLPVKEAPRYPKGNAKAVHPPAAPQRPSLCSSLPCQQLPNASPNQNSHRNHTSANSNHNSIANSSNNAITTKRAEAQSGGRSGAPPDSLLAGLDQPVPEGWTVVREEDFVLVLAIYQSHLAEDLWTAPGASADDGFIHLFYVTAGISRPALLRLFLAMEKGAHLACGCPHLVYEKVRALRLEPISPQGMITVDGEMVEYGPVEAQIHPGLARLVCG

Target ORF information:

RefSeq Version	XM_011603832.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603832.1

ORF Insert Sequence:

ATGACTTTGG AAAATTCCTT CCACTGTGGA ATCGGAAGCT GGGGTGTGGT GTACAGCGAG 
GTGCGTCGGC CCTGCAGGGT CATGATCCTG GTCAACCCTC ACAGCGGCCG CGGCCAGGCC 
CTCCAGCTCT TCAGCGGCCA CGTCCAGGGG ATGCTCACCG AGGCCGCCGT GCCCTACAAG 
CTCGTCATCA CAGAACACCA GAACCACGCA CGGGAGCTGG TGAGGAAGGC CGACCTGTCG 
CAGTGGGACG CTCTGGTCAT CATGTCGGGA GACGGGCTGC TGTTCGAGGT GATCAATGGT 
CTGATGGACA GGGAGGACTG GGAGCAGGCC ATCCAGACCC CTCTGGGCAT CCTGCCGGGG 
GGCTCAGGTA ACGCCCTGGC TGCCTCCGTC CACCACTATT CACAGTCTCC TCCGGCGTGG 
AACGAAGAGC TGCTCCTCAG CTGCGGGTTC ATGCTGTGCA AAGGTCTGGT GGGGCCCCTG 
GACCTGGTCT CCATCCACCT GGCCTCCCAG CAGCGCCTCT TCTCCTTCCT GTCCCTGGCC 
TGGGGCTTCG TGGCCGACGT GGACATCGAG AGCGAGAAGT ACCGTCACGT GGGGGCCATC 
CGCTTCCTGA TGGGCACGCT GGTGCGCCTG GCCACCCTCC GAGTGTACCA GGGCCGGCTG 
GCGTACCTGC CCGTGAAGGA GGCGCCGAGA TACCCCAAAG GGAACGCCAA GGCCGTCCAC 
CCCCCCGCCG CGCCCCAGCG CCCCTCCCTC TGCTCTTCCC TCCCGTGCCA GCAGCTCCCG 
AACGCCTCTC CCAACCAGAA CTCCCACCGC AATCACACGA GCGCCAACTC CAACCACAAC 
AGCATCGCCA ACTCCTCCAA CAACGCCATC ACCACGAAAA GAGCCGAAGC CCAAAGCGGC 
GGCAGATCCG GAGCGCCGCC GGACTCCCTC CTCGCCGGCC TGGACCAGCC GGTCCCGGAG 
GGCTGGACGG TGGTGCGGGA GGAAGACTTT GTGCTGGTCC TGGCCATTTA CCAGTCGCAC 
CTCGCTGAGG ACCTGTGGAC GGCCCCCGGC GCCAGCGCGG ACGACGGCTT CATCCACCTG 
TTCTACGTGA CGGCGGGCAT CTCCCGTCCC GCCCTCCTGC GGCTGTTCCT CGCCATGGAG 
AAGGGCGCCC ACTTGGCGTG CGGCTGCCCT CACCTGGTGT ACGAGAAGGT GAGGGCGCTG 
CGGCTGGAGC CCATCTCCCC GCAGGGCATG ATCACCGTGG ACGGGGAGAT GGTGGAGTAC 
GGGCCGGTGG AGGCCCAGAT CCACCCGGGA CTCGCCAGGC TCGTGTGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062830 ( XM_011603832.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011602134.2 Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo66056
Clone ID Related Accession (Same CDS sequence)	XM_011603832.2
Accession Version	XM_011603832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	sphingosine kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042289.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011603832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGG AAAATTCCTT CCACTGTGGA ATCGGAAGCT GGGGTGTGGT GTACAGCGAG 
GTGCGTCGGC CCTGCAGGGT CATGATCCTG GTCAACCCTC ACAGCGGCCG CGGCCAGGCC 
CTCCAGCTCT TCAGCGGCCA CGTCCAGGGG ATGCTCACCG AGGCCGCCGT GCCCTACAAG 
CTCGTCATCA CAGAACACCA GAACCACGCA CGGGAGCTGG TGAGGAAGGC CGACCTGTCG 
CAGTGGGACG CTCTGGTCAT CATGTCGGGA GACGGGCTGC TGTTCGAGGT GATCAATGGT 
CTGATGGACA GGGAGGACTG GGAGCAGGCC ATCCAGACCC CTCTGGGCAT CCTGCCGGGG 
GGCTCAGGTA ACGCCCTGGC TGCCTCCGTC CACCACTATT CACAGTCTCC TCCGGCGTGG 
AATGAAGAGC TGCTCCTCAG CTGCGGGTTC ATGCTGTGCA AAGGTCTGGT GGGGCCCCTG 
GACCTGGTCT CCATCCACCT GGCCTCCCAG CAGCACCTCT TCTCCTTCCT GTCCCTGGCC 
TGGGGCTTCG TGGCCGACGT GGACATCGAG AGCGAGAAGT ACCGTCACGT GGGGGCCATC 
CGCTTCCTGA TGGGCACGCT GGTGCGCCTG GCCACCCTCC GAGTGTACCA GGGCCGGCTG 
GCGTACCTGC CCGTGAAGGA GGCGCCGAGA TACCCCAAAG GGAACGCCAA GGCCGTCCAC 
CCCCCCGCCG CGCCCCAGCG CCCCTCCCTC TGCTCTTCCC TCCCGTGCCA GCAGCTCCCG 
AACGCCTCTC CCAACCAGAA CTCCCACCGC AATCACACGA GCGCCAACTC CAACCACAAC 
AGCATCGCCA ACTCCTCCAA CAACGCCATC ACCACGAAAA GAGCCGAAGC CCAAAGCGGC 
GGCAGATCCG GAGCGCCGCC GGACTCCCTC CTCGCCGGCC TGGACCAGCC GGTCCCGGAG 
GGCTGGACGG TGGTGCGGGA GGAAGACTTT GTGCTGGTCC TGGCCATTTA CCAGTCGCAC 
CTCGCTGAGG ACCTGTGGAC GGCCCCCGGC GCCAGCGCGG ACGACGGCTT CATCCACCTG 
TTCTACGTGA CGGCGGGCAT CTCCCGTCCC GCCCTCCTGC GGCTGTTCCT CGCCATGGAG 
AAGGGCGCCC ACTTGGCGTG CGGCTGCCCT CACCTGGTGT ACGAGAAGGT GAGGGCGCTG 
CGGCTGGAGC CCATCTCCCC GCAGGGCATG ATCACCGTGG ACGGGGAGAT GGTGGAGTAC 
GGGCCGGTGG AGGCCCAGAT CCACCCGGGA CTCGCCAGGC TCGTGTGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011602134.2
CDS	160..1473
Translation	MTLENSFHCGIGSWGVVYSEVRRPCRVMILVNPHSGRGQALQLFSGHVQGMLTEAAVPYKLVITEHQNHARELVRKADLSQWDALVIMSGDGLLFEVINGLMDREDWEQAIQTPLGILPGGSGNALAASVHHYSQSPPAWNEELLLSCGFMLCKGLVGPLDLVSIHLASQQHLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLATLRVYQGRLAYLPVKEAPRYPKGNAKAVHPPAAPQRPSLCSSLPCQQLPNASPNQNSHRNHTSANSNHNSIANSSNNAITTKRAEAQSGGRSGAPPDSLLAGLDQPVPEGWTVVREEDFVLVLAIYQSHLAEDLWTAPGASADDGFIHLFYVTAGISRPALLRLFLAMEKGAHLACGCPHLVYEKVRALRLEPISPQGMITVDGEMVEYGPVEAQIHPGLARLVCG

Target ORF information:

RefSeq Version	XM_011603832.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603832.2

ORF Insert Sequence:

ATGACTTTGG AAAATTCCTT CCACTGTGGA ATCGGAAGCT GGGGTGTGGT GTACAGCGAG 
GTGCGTCGGC CCTGCAGGGT CATGATCCTG GTCAACCCTC ACAGCGGCCG CGGCCAGGCC 
CTCCAGCTCT TCAGCGGCCA CGTCCAGGGG ATGCTCACCG AGGCCGCCGT GCCCTACAAG 
CTCGTCATCA CAGAACACCA GAACCACGCA CGGGAGCTGG TGAGGAAGGC CGACCTGTCG 
CAGTGGGACG CTCTGGTCAT CATGTCGGGA GACGGGCTGC TGTTCGAGGT GATCAATGGT 
CTGATGGACA GGGAGGACTG GGAGCAGGCC ATCCAGACCC CTCTGGGCAT CCTGCCGGGG 
GGCTCAGGTA ACGCCCTGGC TGCCTCCGTC CACCACTATT CACAGTCTCC TCCGGCGTGG 
AATGAAGAGC TGCTCCTCAG CTGCGGGTTC ATGCTGTGCA AAGGTCTGGT GGGGCCCCTG 
GACCTGGTCT CCATCCACCT GGCCTCCCAG CAGCACCTCT TCTCCTTCCT GTCCCTGGCC 
TGGGGCTTCG TGGCCGACGT GGACATCGAG AGCGAGAAGT ACCGTCACGT GGGGGCCATC 
CGCTTCCTGA TGGGCACGCT GGTGCGCCTG GCCACCCTCC GAGTGTACCA GGGCCGGCTG 
GCGTACCTGC CCGTGAAGGA GGCGCCGAGA TACCCCAAAG GGAACGCCAA GGCCGTCCAC 
CCCCCCGCCG CGCCCCAGCG CCCCTCCCTC TGCTCTTCCC TCCCGTGCCA GCAGCTCCCG 
AACGCCTCTC CCAACCAGAA CTCCCACCGC AATCACACGA GCGCCAACTC CAACCACAAC 
AGCATCGCCA ACTCCTCCAA CAACGCCATC ACCACGAAAA GAGCCGAAGC CCAAAGCGGC 
GGCAGATCCG GAGCGCCGCC GGACTCCCTC CTCGCCGGCC TGGACCAGCC GGTCCCGGAG 
GGCTGGACGG TGGTGCGGGA GGAAGACTTT GTGCTGGTCC TGGCCATTTA CCAGTCGCAC 
CTCGCTGAGG ACCTGTGGAC GGCCCCCGGC GCCAGCGCGG ACGACGGCTT CATCCACCTG 
TTCTACGTGA CGGCGGGCAT CTCCCGTCCC GCCCTCCTGC GGCTGTTCCT CGCCATGGAG 
AAGGGCGCCC ACTTGGCGTG CGGCTGCCCT CACCTGGTGT ACGAGAAGGT GAGGGCGCTG 
CGGCTGGAGC CCATCTCCCC GCAGGGCATG ATCACCGTGG ACGGGGAGAT GGTGGAGTAC 
GGGCCGGTGG AGGCCCAGAT CCACCCGGGA CTCGCCAGGC TCGTGTGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062830 ( XM_003964526.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003964575.1 Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo03532
Clone ID Related Accession (Same CDS sequence)	XM_003964526.2
Accession Version	XM_003964526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	sphingosine kinase 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070955.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003964526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGAAC ACCAGAACCA CGCACGGGAG 
CTGGTGAGGA AGGCCGACCT GTCGCAGTGG GACGCTCTGG TCATCATGTC GGGAGACGGG 
CTGCTGTTCG AGGTGATCAA TGGTCTGATG GACAGGGAGG ACTGGGAGCA GGCCATCCAG 
ACCCCTCTGG GCATCCTGCC GGGGGGCTCA GGTAACGCCC TGGCTGCCTC CGTCCACCAC 
TATTCACAGT CTCCTCCGGC GTGGAACGAA GAGCTGCTCC TCAGCTGCGG GTTCATGCTG 
TGCAAAGGTC TGGTGGGGCC CCTGGACCTG GTCTCCATCC ACCTGGCCTC CCAGCAGCGC 
CTCTTCTCCT TCCTGTCCCT GGCCTGGGGC TTCGTGGCCG ACGTGGACAT CGAGAGCGAG 
AAGTACCGTC ACGTGGGGGC CATCCGCTTC CTGATGGGCA CGCTGGTGCG CCTGGCCACC 
CTCCGAGTGT ACCAGGGCCG GCTGGCGTAC CTGCCCGTGA AGGAGGCGCC GAGATACCCC 
AAAGGGAACG CCAAGGCCGT CCACCCCCCC GCCGCGCCCC AGCGCCCCTC CCTCTGCTCT 
TCCCTCCCGT GCCAGCAGCT CCCGAACGCC TCTCCCAACC AGAACTCCCA CCGCAATCAC 
ACGAGCGCCA ACTCCAACCA CAACAGCATC GCCAACTCCT CCAACAACGC CATCACCACG 
AAAAGAGCCG AAGCCCAAAG CGGCGGCAGA TCCGGAGCGC CGCCGGACTC CCTCCTCGCC 
GGCCTGGACC AGCCGGTCCC GGAGGGCTGG ACGGTGGTGC GGGAGGAAGA CTTTGTGCTG 
GTCCTGGCCA TTTACCAGTC GCACCTCGCT GAGGACCTGT GGACGGCCCC CGGCGCCAGC 
GCGGACGACG GCTTCATCCA CCTGTTCTAC GTGACGGCGG GCATCTCCCG TCCCGCCCTC 
CTGCGGCTGT TCCTCGCCAT GGAGAAGGGC GCCCACTTGG CGTGCGGCTG CCCTCACCTG 
GTGTACGAGA AGGTGAGGGC GCTGCGGCTG GAGCCCATCT CCCCGCAGGG CATGATCACC 
GTGGACGGGG AGATGGTGGA GTACGGGCCG GTGGAGGCCC AGATCCACCC GGGACTCGCC 
AGGCTCGTGT GCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964575.1
CDS	561..2258
Translation	MEKDASEPDPSRHRNGPVGVLYGEFTDMLNDRVRYSVSLTESALSIQKISASPGRTKVVFHLADCVGCRAHGGPDGADAGAYFTAYFYPFKRRWMSAGVARQRVEQCFRVALVQDPVANLREAEMWARAIREASALQAPRRDGVVYSEVRRPCRVMILVNPHSGRGQALQLFSGHVQGMLTEAAVPYKLVITEHQNHARELVRKADLSQWDALVIMSGDGLLFEVINGLMDREDWEQAIQTPLGILPGGSGNALAASVHHYSQSPPAWNEELLLSCGFMLCKGLVGPLDLVSIHLASQQRLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLATLRVYQGRLAYLPVKEAPRYPKGNAKAVHPPAAPQRPSLCSSLPCQQLPNASPNQNSHRNHTSANSNHNSIANSSNNAITTKRAEAQSGGRSGAPPDSLLAGLDQPVPEGWTVVREEDFVLVLAIYQSHLAEDLWTAPGASADDGFIHLFYVTAGISRPALLRLFLAMEKGAHLACGCPHLVYEKVRALRLEPISPQGMITVDGEMVEYGPVEAQIHPGLARLVCG

Target ORF information:

RefSeq Version	XM_003964526.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes sphingosine kinase 1-like (LOC101062830), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964526.2

ORF Insert Sequence:

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGAAC ACCAGAACCA CGCACGGGAG 
CTGGTGAGGA AGGCCGACCT GTCGCAGTGG GACGCTCTGG TCATCATGTC GGGAGACGGG 
CTGCTGTTCG AGGTGATCAA TGGTCTGATG GACAGGGAGG ACTGGGAGCA GGCCATCCAG 
ACCCCTCTGG GCATCCTGCC GGGGGGCTCA GGTAACGCCC TGGCTGCCTC CGTCCACCAC 
TATTCACAGT CTCCTCCGGC GTGGAACGAA GAGCTGCTCC TCAGCTGCGG GTTCATGCTG 
TGCAAAGGTC TGGTGGGGCC CCTGGACCTG GTCTCCATCC ACCTGGCCTC CCAGCAGCGC 
CTCTTCTCCT TCCTGTCCCT GGCCTGGGGC TTCGTGGCCG ACGTGGACAT CGAGAGCGAG 
AAGTACCGTC ACGTGGGGGC CATCCGCTTC CTGATGGGCA CGCTGGTGCG CCTGGCCACC 
CTCCGAGTGT ACCAGGGCCG GCTGGCGTAC CTGCCCGTGA AGGAGGCGCC GAGATACCCC 
AAAGGGAACG CCAAGGCCGT CCACCCCCCC GCCGCGCCCC AGCGCCCCTC CCTCTGCTCT 
TCCCTCCCGT GCCAGCAGCT CCCGAACGCC TCTCCCAACC AGAACTCCCA CCGCAATCAC 
ACGAGCGCCA ACTCCAACCA CAACAGCATC GCCAACTCCT CCAACAACGC CATCACCACG 
AAAAGAGCCG AAGCCCAAAG CGGCGGCAGA TCCGGAGCGC CGCCGGACTC CCTCCTCGCC 
GGCCTGGACC AGCCGGTCCC GGAGGGCTGG ACGGTGGTGC GGGAGGAAGA CTTTGTGCTG 
GTCCTGGCCA TTTACCAGTC GCACCTCGCT GAGGACCTGT GGACGGCCCC CGGCGCCAGC 
GCGGACGACG GCTTCATCCA CCTGTTCTAC GTGACGGCGG GCATCTCCCG TCCCGCCCTC 
CTGCGGCTGT TCCTCGCCAT GGAGAAGGGC GCCCACTTGG CGTGCGGCTG CCCTCACCTG 
GTGTACGAGA AGGTGAGGGC GCTGCGGCTG GAGCCCATCT CCCCGCAGGG CATGATCACC 
GTGGACGGGG AGATGGTGGA GTACGGGCCG GTGGAGGCCC AGATCCACCC GGGACTCGCC 
AGGCTCGTGT GCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062830 ( XM_003964526.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003964575.2 Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X1, mRNA.

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CloneID	OTo66054
Clone ID Related Accession (Same CDS sequence)	XM_003964526.3
Accession Version	XM_003964526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	sphingosine kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042289.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003964526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGAAC ACCAGAACCA CGCACGGGAG 
CTGGTGAGGA AGGCCGACCT GTCGCAGTGG GACGCTCTGG TCATCATGTC GGGAGACGGG 
CTGCTGTTCG AGGTGATCAA TGGTCTGATG GACAGGGAGG ACTGGGAGCA GGCCATCCAG 
ACCCCTCTGG GCATCCTGCC GGGGGGCTCA GGTAACGCCC TGGCTGCCTC CGTCCACCAC 
TATTCACAGT CTCCTCCGGC GTGGAATGAA GAGCTGCTCC TCAGCTGCGG GTTCATGCTG 
TGCAAAGGTC TGGTGGGGCC CCTGGACCTG GTCTCCATCC ACCTGGCCTC CCAGCAGCAC 
CTCTTCTCCT TCCTGTCCCT GGCCTGGGGC TTCGTGGCCG ACGTGGACAT CGAGAGCGAG 
AAGTACCGTC ACGTGGGGGC CATCCGCTTC CTGATGGGCA CGCTGGTGCG CCTGGCCACC 
CTCCGAGTGT ACCAGGGCCG GCTGGCGTAC CTGCCCGTGA AGGAGGCGCC GAGATACCCC 
AAAGGGAACG CCAAGGCCGT CCACCCCCCC GCCGCGCCCC AGCGCCCCTC CCTCTGCTCT 
TCCCTCCCGT GCCAGCAGCT CCCGAACGCC TCTCCCAACC AGAACTCCCA CCGCAATCAC 
ACGAGCGCCA ACTCCAACCA CAACAGCATC GCCAACTCCT CCAACAACGC CATCACCACG 
AAAAGAGCCG AAGCCCAAAG CGGCGGCAGA TCCGGAGCGC CGCCGGACTC CCTCCTCGCC 
GGCCTGGACC AGCCGGTCCC GGAGGGCTGG ACGGTGGTGC GGGAGGAAGA CTTTGTGCTG 
GTCCTGGCCA TTTACCAGTC GCACCTCGCT GAGGACCTGT GGACGGCCCC CGGCGCCAGC 
GCGGACGACG GCTTCATCCA CCTGTTCTAC GTGACGGCGG GCATCTCCCG TCCCGCCCTC 
CTGCGGCTGT TCCTCGCCAT GGAGAAGGGC GCCCACTTGG CGTGCGGCTG CCCTCACCTG 
GTGTACGAGA AGGTGAGGGC GCTGCGGCTG GAGCCCATCT CCCCGCAGGG CATGATCACC 
GTGGACGGGG AGATGGTGGA GTACGGGCCG GTGGAGGCCC AGATCCACCC GGGACTCGCC 
AGGCTCGTGT GCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964575.2
CDS	275..1972
Translation	MEKDASEPDPSRHRNGPVGVLYGEFTDMLNDRVRYSVSLTESALSIQKISASPGRTKVVFHLADCVGCRAHGGPDGADAGAYFTAYFYPFKRRWMSAGVARQRVEQCFRVALVQDPVANLREAEMWARAIREASALQAPRRDGVVYSEVRRPCRVMILVNPHSGRGQALQLFSGHVQGMLTEAAVPYKLVITEHQNHARELVRKADLSQWDALVIMSGDGLLFEVINGLMDREDWEQAIQTPLGILPGGSGNALAASVHHYSQSPPAWNEELLLSCGFMLCKGLVGPLDLVSIHLASQQHLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLATLRVYQGRLAYLPVKEAPRYPKGNAKAVHPPAAPQRPSLCSSLPCQQLPNASPNQNSHRNHTSANSNHNSIANSSNNAITTKRAEAQSGGRSGAPPDSLLAGLDQPVPEGWTVVREEDFVLVLAIYQSHLAEDLWTAPGASADDGFIHLFYVTAGISRPALLRLFLAMEKGAHLACGCPHLVYEKVRALRLEPISPQGMITVDGEMVEYGPVEAQIHPGLARLVCG

Target ORF information:

RefSeq Version	XM_003964526.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964526.3

ORF Insert Sequence:

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGAAC ACCAGAACCA CGCACGGGAG 
CTGGTGAGGA AGGCCGACCT GTCGCAGTGG GACGCTCTGG TCATCATGTC GGGAGACGGG 
CTGCTGTTCG AGGTGATCAA TGGTCTGATG GACAGGGAGG ACTGGGAGCA GGCCATCCAG 
ACCCCTCTGG GCATCCTGCC GGGGGGCTCA GGTAACGCCC TGGCTGCCTC CGTCCACCAC 
TATTCACAGT CTCCTCCGGC GTGGAATGAA GAGCTGCTCC TCAGCTGCGG GTTCATGCTG 
TGCAAAGGTC TGGTGGGGCC CCTGGACCTG GTCTCCATCC ACCTGGCCTC CCAGCAGCAC 
CTCTTCTCCT TCCTGTCCCT GGCCTGGGGC TTCGTGGCCG ACGTGGACAT CGAGAGCGAG 
AAGTACCGTC ACGTGGGGGC CATCCGCTTC CTGATGGGCA CGCTGGTGCG CCTGGCCACC 
CTCCGAGTGT ACCAGGGCCG GCTGGCGTAC CTGCCCGTGA AGGAGGCGCC GAGATACCCC 
AAAGGGAACG CCAAGGCCGT CCACCCCCCC GCCGCGCCCC AGCGCCCCTC CCTCTGCTCT 
TCCCTCCCGT GCCAGCAGCT CCCGAACGCC TCTCCCAACC AGAACTCCCA CCGCAATCAC 
ACGAGCGCCA ACTCCAACCA CAACAGCATC GCCAACTCCT CCAACAACGC CATCACCACG 
AAAAGAGCCG AAGCCCAAAG CGGCGGCAGA TCCGGAGCGC CGCCGGACTC CCTCCTCGCC 
GGCCTGGACC AGCCGGTCCC GGAGGGCTGG ACGGTGGTGC GGGAGGAAGA CTTTGTGCTG 
GTCCTGGCCA TTTACCAGTC GCACCTCGCT GAGGACCTGT GGACGGCCCC CGGCGCCAGC 
GCGGACGACG GCTTCATCCA CCTGTTCTAC GTGACGGCGG GCATCTCCCG TCCCGCCCTC 
CTGCGGCTGT TCCTCGCCAT GGAGAAGGGC GCCCACTTGG CGTGCGGCTG CCCTCACCTG 
GTGTACGAGA AGGTGAGGGC GCTGCGGCTG GAGCCCATCT CCCCGCAGGG CATGATCACC 
GTGGACGGGG AGATGGTGGA GTACGGGCCG GTGGAGGCCC AGATCCACCC GGGACTCGCC 
AGGCTCGTGT GCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062830 ( XM_029835917.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029691777.1 Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X2, mRNA.

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CloneID	OTo66055
Clone ID Related Accession (Same CDS sequence)	XM_029835917.1
Accession Version	XM_029835917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	sphingosine kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042289.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGGTG ATCAATGGTC TGATGGACAG 
GGAGGACTGG GAGCAGGCCA TCCAGACCCC TCTGGGCATC CTGCCGGGGG GCTCAGGTCT 
CCTCCGGCGT GGAATGAAGA GCTGCTCCTC AGCTGCGGGT TCATGCTGTG CAAAGGTCTG 
GTGGGGCCCC TGGACCTGGT CTCCATCCAC CTGGCCTCCC AGCAGCACCT CTTCTCCTTC 
CTGTCCCTGG CCTGGGGCTT CGTGGCCGAC GTGGACATCG AGAGCGAGAA GTACCGTCAC 
GTGGGGGCCA TCCGCTTCCT GATGGGCACG CTGGTGCGCC TGGCCACCCT CCGAGTGTAC 
CAGGGCCGGC TGGCGTACCT GCCCGTGAAG GAGGCGCCGA GATACCCCAA AGGGAACGCC 
AAGGCCGTCC ACCCCCCCGC CGCGCCCCAG CGCCCCTCCC TCTGCTCTTC CCTCCCGTGC 
CAGCAGCTCC CGAACGCCTC TCCCAACCAG AACTCCCACC GCAATCACAC GAGCGCCAAC 
TCCAACCACA ACAGCATCGC CAACTCCTCC AACAACGCCA TCACCACGAA AAGAGCCGAA 
GCCCAAAGCG GCGGCAGATC CGGAGCGCCG CCGGACTCCC TCCTCGCCGG CCTGGACCAG 
CCGGTCCCGG AGGGCTGGAC GGTGGTGCGG GAGGAAGACT TTGTGCTGGT CCTGGCCATT 
TACCAGTCGC ACCTCGCTGA GGACCTGTGG ACGGCCCCCG GCGCCAGCGC GGACGACGGC 
TTCATCCACC TGTTCTACGT GACGGCGGGC ATCTCCCGTC CCGCCCTCCT GCGGCTGTTC 
CTCGCCATGG AGAAGGGCGC CCACTTGGCG TGCGGCTGCC CTCACCTGGT GTACGAGAAG 
GTGAGGGCGC TGCGGCTGGA GCCCATCTCC CCGCAGGGCA TGATCACCGT GGACGGGGAG 
ATGGTGGAGT ACGGGCCGGT GGAGGCCCAG ATCCACCCGG GACTCGCCAG GCTCGTGTGC 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029691777.1
CDS	278..1843
Translation	MEKDASEPDPSRHRNGPVGVLYGEFTDMLNDRVRYSVSLTESALSIQKISASPGRTKVVFHLADCVGCRAHGGPDGADAGAYFTAYFYPFKRRWMSAGVARQRVEQCFRVALVQDPVANLREAEMWARAIREASALQAPRRDGVVYSEVRRPCRVMILVNPHSGRGQALQLFSGHVQGMLTEAAVPYKLVITGDQWSDGQGGLGAGHPDPSGHPAGGLRSPPAWNEELLLSCGFMLCKGLVGPLDLVSIHLASQQHLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLATLRVYQGRLAYLPVKEAPRYPKGNAKAVHPPAAPQRPSLCSSLPCQQLPNASPNQNSHRNHTSANSNHNSIANSSNNAITTKRAEAQSGGRSGAPPDSLLAGLDQPVPEGWTVVREEDFVLVLAIYQSHLAEDLWTAPGASADDGFIHLFYVTAGISRPALLRLFLAMEKGAHLACGCPHLVYEKVRALRLEPISPQGMITVDGEMVEYGPVEAQIHPGLARLVCG

Target ORF information:

RefSeq Version	XM_029835917.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes sphingosine kinase 1 (sphk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029835917.1

ORF Insert Sequence:

ATGGAGAAAG ACGCATCTGA ACCGGACCCA TCCCGCCACC GGAACGGGCC GGTGGGCGTG 
TTGTACGGGG AGTTCACCGA CATGCTGAAC GACAGGGTCC GGTACTCGGT CAGCTTGACG 
GAGAGCGCCC TGTCCATCCA GAAGATCTCC GCGTCACCCG GCCGGACGAA GGTAGTGTTT 
CACCTGGCGG ACTGTGTCGG CTGCCGGGCG CACGGAGGGC CGGACGGCGC GGACGCCGGG 
GCCTACTTCA CCGCCTATTT CTACCCGTTC AAGAGGCGGT GGATGAGCGC CGGGGTGGCC 
CGGCAGAGAG TGGAGCAGTG CTTTCGGGTC GCGCTGGTCC AGGACCCGGT CGCGAACCTG 
CGGGAGGCGG AGATGTGGGC TCGGGCCATC AGGGAGGCTT CGGCGCTGCA GGCGCCCCGG 
AGAGACGGTG TGGTGTACAG CGAGGTGCGT CGGCCCTGCA GGGTCATGAT CCTGGTCAAC 
CCTCACAGCG GCCGCGGCCA GGCCCTCCAG CTCTTCAGCG GCCACGTCCA GGGGATGCTC 
ACCGAGGCCG CCGTGCCCTA CAAGCTCGTC ATCACAGGTG ATCAATGGTC TGATGGACAG 
GGAGGACTGG GAGCAGGCCA TCCAGACCCC TCTGGGCATC CTGCCGGGGG GCTCAGGTCT 
CCTCCGGCGT GGAATGAAGA GCTGCTCCTC AGCTGCGGGT TCATGCTGTG CAAAGGTCTG 
GTGGGGCCCC TGGACCTGGT CTCCATCCAC CTGGCCTCCC AGCAGCACCT CTTCTCCTTC 
CTGTCCCTGG CCTGGGGCTT CGTGGCCGAC GTGGACATCG AGAGCGAGAA GTACCGTCAC 
GTGGGGGCCA TCCGCTTCCT GATGGGCACG CTGGTGCGCC TGGCCACCCT CCGAGTGTAC 
CAGGGCCGGC TGGCGTACCT GCCCGTGAAG GAGGCGCCGA GATACCCCAA AGGGAACGCC 
AAGGCCGTCC ACCCCCCCGC CGCGCCCCAG CGCCCCTCCC TCTGCTCTTC CCTCCCGTGC 
CAGCAGCTCC CGAACGCCTC TCCCAACCAG AACTCCCACC GCAATCACAC GAGCGCCAAC 
TCCAACCACA ACAGCATCGC CAACTCCTCC AACAACGCCA TCACCACGAA AAGAGCCGAA 
GCCCAAAGCG GCGGCAGATC CGGAGCGCCG CCGGACTCCC TCCTCGCCGG CCTGGACCAG 
CCGGTCCCGG AGGGCTGGAC GGTGGTGCGG GAGGAAGACT TTGTGCTGGT CCTGGCCATT 
TACCAGTCGC ACCTCGCTGA GGACCTGTGG ACGGCCCCCG GCGCCAGCGC GGACGACGGC 
TTCATCCACC TGTTCTACGT GACGGCGGGC ATCTCCCGTC CCGCCCTCCT GCGGCTGTTC 
CTCGCCATGG AGAAGGGCGC CCACTTGGCG TGCGGCTGCC CTCACCTGGT GTACGAGAAG 
GTGAGGGCGC TGCGGCTGGA GCCCATCTCC CCGCAGGGCA TGATCACCGT GGACGGGGAG 
ATGGTGGAGT ACGGGCCGGT GGAGGCCCAG ATCCACCCGG GACTCGCCAG GCTCGTGTGC 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101062830 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム