IFNG cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFNG
Entrez Gene ID	396991
Full Name	interferon gamma
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 5 Map Location: 5\|5p1.2-q1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_213948.1	NP_999113.1	interferon gamma precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

IFN-? regulates cytochrome 3A29 through pregnane X receptor in pigs.
Li X, Hu X, Jin X, Zhou X, Wang X, Shi D, Bi D
Xenobiotica; the fate of foreign compounds in biological systems45(5)373-9(2015 May)

Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F(2) Duroc x Pietrain resource population.
Uddin MJ, Grosse-Brinkhaus C, Cinar MU, Jonas E, Tesfaye D, Tholen E, Juengst H, Looft C, Ponsuksili S, Wimmers K, Phatsara C, Schellander K
Mammalian genome : official journal of the International Mammalian Genome Society21(7-8)409-18(2010 Aug)

Effect of cryopreservation on IL-4, IFNgamma and IL-6 production of porcine peripheral blood lymphocytes.
Li X, Zhong Z, Liang S, Wang X, Zhong F
Cryobiology59(3)322-6(2009 Dec)

Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactis.
Rupa P, Monedero V, Wilkie BN
Veterinary microbiology129(1-2)197-202(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR.
Title: IFN-? regulates cytochrome 3A29 through pregnane X receptor in pigs.

Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma.
Title: Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F(2) Duroc x Pietrain resource population.

IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones.
Title: Effect of cryopreservation on IL-4, IFNgamma and IL-6 production of porcine peripheral blood lymphocytes.

The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported.
Title: Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactis.

Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA.
Title: [Expression of porcine gamma-interferon in recombinant baculovirus and determination of its antiviral activity].

The following IFNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe30424	NM_213948.1 Latest version!	Sus scrofa interferon gamma (IFNG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFNG ( NM_213948.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999113.1 Sus scrofa interferon gamma (IFNG), mRNA.

Price & Availability↑

CloneID	OSe30424
Clone ID Related Accession (Same CDS sequence)	NM_213948.1
Accession Version	NM_213948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Sus scrofa(pig)
Product	interferon gamma precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY188090.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY188090.1, DQ839398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3180005, SAMN01915405 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTATA CAACTTATTT CTTAGCTTTT CAGCTTTGCG TGACTTTGTG TTTTTCTGGC 
TCTTACTGCC AGGCGCCCTT TTTTAAAGAA ATAACGATCC TAAAGGACTA TTTTAATGCA 
AGTACCTCAG ATGTACCTAA TGGTGGACCT CTTTTCTTAG AAATTTTGAA GAATTGGAAA 
GAGGAGAGTG ACAAAAAAAT AATTCAGAGC CAAATTGTCT CCTTCTACTT CAAATTCTTT 
GAAATCTTCA AAGATAACCA GGCCATTCAA AGGAGCATGG ATGTGATCAA GCAAGACATG 
TTTCAGAGGT TCCTAAATGG TAGCTCTGGG AAACTGAATG ACTTCGAAAA GCTGATTAAA 
ATTCCGGTAG ATAATCTGCA GATCCAGCGC AAAGCCATCA GTGAACTCAT CAAAGTGATG 
AATGATCTGT CACCAAGATC TAACCTAAGA AAGCGGAAGA GAAGTCAGAC TATGTTCCAA 
GGCCAGAGAG CATCAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999113.1
CDS	25..525
Translation	MSYTTYFLAFQLCVTLCFSGSYCQAPFFKEITILKDYFNASTSDVPNGGPLFLEILKNWKEESDKKIIQSQIVSFYFKFFEIFKDNQAIQRSMDVIKQDMFQRFLNGSSGKLNDFEKLIKIPVDNLQIQRKAISELIKVMNDLSPRSNLRKRKRSQTMFQGQRASK

Target ORF information:

RefSeq Version	NM_213948.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa interferon gamma (IFNG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213948.1

ORF Insert Sequence:

ATGAGTTATA CAACTTATTT CTTAGCTTTT CAGCTTTGCG TGACTTTGTG TTTTTCTGGC 
TCTTACTGCC AGGCGCCCTT TTTTAAAGAA ATAACGATCC TAAAGGACTA TTTTAATGCA 
AGTACCTCAG ATGTACCTAA TGGTGGACCT CTTTTCTTAG AAATTTTGAA GAATTGGAAA 
GAGGAGAGTG ACAAAAAAAT AATTCAGAGC CAAATTGTCT CCTTCTACTT CAAATTCTTT 
GAAATCTTCA AAGATAACCA GGCCATTCAA AGGAGCATGG ATGTGATCAA GCAAGACATG 
TTTCAGAGGT TCCTAAATGG TAGCTCTGGG AAACTGAATG ACTTCGAAAA GCTGATTAAA 
ATTCCGGTAG ATAATCTGCA GATCCAGCGC AAAGCCATCA GTGAACTCAT CAAAGTGATG 
AATGATCTGT CACCAAGATC TAACCTAAGA AAGCGGAAGA GAAGTCAGAC TATGTTCCAA 
GGCCAGAGAG CATCAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IFN-? regulates cytochrome 3A29 through pregnane X receptor in pigs.
Xenobiotica; the fate of foreign compounds in biological systems45(5)373-9(2015 May)
Li X,Hu X,Jin X,Zhou X,Wang X,Shi D,Bi D

Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F(2) Duroc x Pietrain resource population.
Mammalian genome : official journal of the International Mammalian Genome Society21(7-8)409-18(2010 Aug)
Uddin MJ,Grosse-Brinkhaus C,Cinar MU,Jonas E,Tesfaye D,Tholen E,Juengst H,Looft C,Ponsuksili S,Wimmers K,Phatsara C,Schellander K

Effect of cryopreservation on IL-4, IFNgamma and IL-6 production of porcine peripheral blood lymphocytes.
Cryobiology59(3)322-6(2009 Dec)
Li X,Zhong Z,Liang S,Wang X,Zhong F

Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactis.
Veterinary microbiology129(1-2)197-202(2008 May)
Rupa P,Monedero V,Wilkie BN