CXCL8 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	CXCL8
Entrez Gene ID	396880
Full Name	C-X-C motif chemokine ligand 8
Synonyms	AMCF-I,IL8
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213867.1	NP_999032.1	interleukin-8 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 expression through TAK-1/JNK/AP-1 pathways.
Liu Y, Du Y, Wang H, Du L, Feng WH
Virology50664-72(2017 06)

TLR2/MyD88/NF-?B signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2.
Qin Y, Li H, Qiao J
The Journal of general virology97(2)445-52(2016 Feb)

The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction.
Choi CY, Rho SB, Kim HS, Han J, Bae J, Lee SJ, Jung WW, Chun T
The Journal of general virology96(Pt 5)1098-108(2015 May)

Transforming growth factor-?2 and endotoxin interact to regulate homeostasis via interleukin-8 levels in the immature intestine.
Nguyen DN, Sangild PT, Ostergaard MV, Bering SB, Chatterton DE
American journal of physiology. Gastrointestinal and liver physiology307(7)G689-99(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV
Title: Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 expression through TAK-1/JNK/AP-1 pathways.

These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway.
Title: TLR2/MyD88/NF-?B signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2.

Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts.
Title: The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction.

Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine.
Title: Transforming growth factor-?2 and endotoxin interact to regulate homeostasis via interleukin-8 levels in the immature intestine.

Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production.
Title: Lysophosphatidic acid-induced IL-8 secretion involves MSK1 and MSK2 mediated activation of CREB1 in human fibroblast-like synoviocytes.

The following CXCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CXCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00837	NM_213867.1 Latest version!	Sus scrofa C-X-C motif chemokine ligand 8 (CXCL8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CXCL8 ( NM_213867.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999032.1 Sus scrofa C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Price & Availability↑

CloneID	OSe00837
Clone ID Related Accession (Same CDS sequence)	NM_213867.1
Accession Version	NM_213867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Sus scrofa(pig)
Product	interleukin-8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M86923.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M86923.1, M99367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2041641, SAMEA3179994 [ECO:0000348] ##Evidence-Data-END##

ATGACTTCCA AACTGGCTGT TGCCTTCTTG GCAGTTTTCC TGCTTTCTGC AGCTCTCTGT 
GAGGCTGCAG TTCTGGCAAG AGTAAGTGCA GAACTTCGAT GCCAGTGCAT AAATACGCAT 
TCCACACCTT TCCACCCCAA ATTTATCAAG GAACTGAGAG TGATTGAGAG TGGACCCCAC 
TGTGAAAATT CAGAAATCAT TGTTAAGCTT GTCAATGGAA AAGAGGTCTG CCTGGACCCC 
AAGGAAAAGT GGGTGCAGAA GGTTGTACAG ATATTTTTGA AGAGAACTGA GAAGCAACAA 
CAACAGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999032.1
CDS	84..395
Translation	MTSKLAVAFLAVFLLSAALCEAAVLARVSAELRCQCINTHSTPFHPKFIKELRVIESGPHCENSEIIVKLVNGKEVCLDPKEKWVQKVVQIFLKRTEKQQQQQ

Target ORF information:

RefSeq Version	NM_213867.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213867.1

ORF Insert Sequence:

ATGACTTCCA AACTGGCTGT TGCCTTCTTG GCAGTTTTCC TGCTTTCTGC AGCTCTCTGT 
GAGGCTGCAG TTCTGGCAAG AGTAAGTGCA GAACTTCGAT GCCAGTGCAT AAATACGCAT 
TCCACACCTT TCCACCCCAA ATTTATCAAG GAACTGAGAG TGATTGAGAG TGGACCCCAC 
TGTGAAAATT CAGAAATCAT TGTTAAGCTT GTCAATGGAA AAGAGGTCTG CCTGGACCCC 
AAGGAAAAGT GGGTGCAGAA GGTTGTACAG ATATTTTTGA AGAGAACTGA GAAGCAACAA 
CAACAGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 expression through TAK-1/JNK/AP-1 pathways.
Virology50664-72(2017 06)
Liu Y,Du Y,Wang H,Du L,Feng WH

TLR2/MyD88/NF-?B signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2.
The Journal of general virology97(2)445-52(2016 Feb)
Qin Y,Li H,Qiao J

The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction.
The Journal of general virology96(Pt 5)1098-108(2015 May)
Choi CY,Rho SB,Kim HS,Han J,Bae J,Lee SJ,Jung WW,Chun T

Transforming growth factor-?2 and endotoxin interact to regulate homeostasis via interleukin-8 levels in the immature intestine.
American journal of physiology. Gastrointestinal and liver physiology307(7)G689-99(2014 Oct)
Nguyen DN,Sangild PT,Ostergaard MV,Bering SB,Chatterton DE