LOC100522732 gene cDNA ORF clone, Sus scrofa(Pig)

mRNA	Protein	Name
XM_013996042.2	XP_013851496.2	LOW QUALITY PROTEIN: dysferlin
XM_013996038.1	XP_013851492.1	dysferlin isoform X1
XM_013996042.1	XP_013851496.1	dysferlin isoform X5
XM_013996052.1	XP_013851506.1	dysferlin isoform X15
XM_013996054.1	XP_013851508.1	dysferlin isoform X17
XM_013996056.1	XP_013851510.1	dysferlin isoform X19
XM_013996053.1	XP_013851507.1	dysferlin isoform X16
XM_013996040.1	XP_013851494.1	dysferlin isoform X3
XM_013996045.1	XP_013851499.1	dysferlin isoform X8
XM_013996046.1	XP_013851500.1	dysferlin isoform X9
XM_013996057.1	XP_013851511.1	dysferlin isoform X20
XM_013996041.1	XP_013851495.1	dysferlin isoform X4
XM_013996043.1	XP_013851497.1	dysferlin isoform X6
XM_013996044.1	XP_013851498.1	dysferlin isoform X7
XM_013996051.1	XP_013851505.1	dysferlin isoform X14
XM_013996039.1	XP_013851493.1	dysferlin isoform X2
XM_013996047.1	XP_013851501.1	dysferlin isoform X10
XM_013996048.1	XP_013851502.1	dysferlin isoform X11
XM_013996049.1	XP_013851503.1	dysferlin isoform X12
XM_013996050.1	XP_013851504.1	dysferlin isoform X13
XM_013996055.1	XP_013851509.1	dysferlin isoform X18

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe34210	XM_013996048.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe34212	XM_013996050.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe34216	XM_013996054.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe34217	XM_013996055.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe34218	XM_013996056.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe34219	XM_013996057.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe246348	XM_013996042.2 Latest version!	Sus scrofa dysferlin (DYSF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34200	XM_013996038.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34201	XM_013996039.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34202	XM_013996040.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34203	XM_013996041.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34204	XM_013996042.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34205	XM_013996043.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34206	XM_013996044.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34207	XM_013996045.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34208	XM_013996046.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34209	XM_013996047.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34211	XM_013996049.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34213	XM_013996051.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34214	XM_013996052.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSe34215	XM_013996053.1 Latest version!	Sus scrofa dysferlin (LOC100522732), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

LOC100522732 ( XM_013996048.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851502.1 Sus scrofa dysferlin (LOC100522732), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSe34210
Clone ID Related Accession (Same CDS sequence)	XM_013996048.1
Accession Version	XM_013996048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGT GGACGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TACGTCAACC TGTACGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGACCC CTACGCGGAG CTCAACACAG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGCC GGCTGCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGAGCAGAAG 
GTGGAGGATC TTCCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTAC AGGACGTGGA CGACGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTACGGG AACAAGTTCG ACACGACCTG CCTGCCTCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACCGA 
GTGGAGGCTC AGAACCAGCT GCTCAGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG 
GTTCACTTGG CCCTGAGGGC GCAGTGCTCC ACCGAGGACG TGGACTCCCT GGCGGCTCAG 
CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTAG GTGACATCCA TGAGACGCCC 
TCTGCTACCC ACCTGGACCA GTACCTGCAC CGGCTGCGTA TGCGTCACCT GAGCCAGATC 
GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC CACAGTGAAC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCAGATG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT GCAGGGCGAC AAGCGGGTGG CCTACCAGCG GGTGCCTGCC 
CACGAGGTCC TCTTCTCCCG GCGGGGCTCC AGCTACTGTG GCAAGAATTG TGGAAAGCTG 
CAGACGATAT TTCTGAAATA TCCGATGGAG GGGATGCCCA GAGCCAGGAT GCCAGTGCAG 
ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC GTGGATGAAA AGGAGTTCAA TCAGTTTGCA 
GAGGGCAAGC TCTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAGCT GGCCCTGGTC 
GGAAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC GGGCAAGATC 
AAGCTCCCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGA CCTGGGCTGG AGACTGGTTC 
GTGTGTCCGG AGAAGACCCT GCTTCATGAC ACTGATGCGG GCCATCTGAG CTTCGTGGAG 
GAGGTGTTTG AGAACCAAAC CCGGCTCCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAATG CCCACTGGGC 
TGGAAGTGGG AAGATGAGGA GTGGTCCACG GACCTCAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCTCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCTGAG 
AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC 
GCCTCACTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGACGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG GAGAAGACGG GACCTGCAGC TGTGTTCGCC 
CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC GACAGGAGTG AGGATTCCAT GTCTGTCTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATCTCCTGCA TCTTCGACTA TGGAAACCGC 
TACCATCTAC GTTGCTATAT GTACCAGGCC CGGGATCTGC CTGCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAATACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTACGAGAT TGAGATCTTC 
GGCGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTACG GCGCAGATGA GTTCATGGGA CGCTGCATCT GTCAGCCGAG TCTGGAACGG 
ATGCCCCGGT TGGCCTGGTT CCCTCTGACT AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCTATCC ACCATATTCC CGGTTTTGAG 
GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CGGACCTGCC CTACCCGCCA 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACAGCCATTG AGATCCTGGC ATGGGGGCTT CGGAACATGA AGAGTTACCA GCTGGCCAGT 
GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCCGGGAGG ATCTCTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGGCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGACGTGAG CTTGCTCAGT 
CCTGGGGAAG ACGTGCTGAT TGACATCGAC GACAAGGAAC CCCTCATCCC CATCCAGCTT 
GCGGATGGTC TGTCGGGCTT GGCCCCCACT AACATGGCGT CTTCTCCATC CAGTCCTCAT 
GAGGAGGAGT TTATTGACTG GTGGAGCAAA TTCTTCGCCT CGATTGGGGA GAGGGAAAAG 
TGTGGGTCCT ACCTGGAGAA GGACTTTGAC ACTCTAAAGG TCTATGATAC CCCGCTGGAG 
AATGTGAAGG CTTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGATGGA AGACCCATCT GTCATTGGGG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCTGCCATT CCCATGCCTC CAAGACAGTT CCACCAGCTG 
GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC CGCATCTACA TCATCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGCG ACCAAGACAA CTACATCCCC TGCACCCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACCCT CTATGACTAC 
GACCTCCTCT CCAAGGATGA AAAGATCGGG GAGACGGTCA TCGACCTGGA GAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC CCCCAGACCT ATTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG CTTCTCCACC TGTTCTGCCA GCAGCACAGG 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCATT 
GAGGAAATAG AGGCTGGCAG GGTCCCAAAC CCACACCTGG GCCCAGCAGA GGAGCGTCTG 
GCCTTGTATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC 
TATAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGAT CGACCTGTTT 
CCAAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGGAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TTCTGGATGA CCTCAGCATC 
ACCGGGGAGA AGATGAGTGA CATTTATGTG AAAGGCTGGA TGGTGGGCTT TGAAGAACAC 
AAGCAGAAGA CAGATGTGCA TTATCGGTCC CTGGGAGGCG AAGGCAACTT TAACTGGAGG 
TTCGTTTTCC CTTTCGACTA CCTGCCAGCT GAGCAAGTCT GCACCGTCTC CAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAGATCCCTG CGCGAGTAGT CTTCCAGATT 
TGGGACAACG ACAAGTTCTC CTTTGATGAC TTTTTGGGTG AGCACCATTT CTGGTCTGTA 
CCACTCTCCA CTCGGGGCTG CCCCAGAACC CACGCTTTGC GGTGTTGGAT GGTAAGACTC 
AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG CAGTGCAGGC TCCAGTGGGC CGTCACTGGC 
TGGCTGGCCT CTCACTTGAC AGGATCTCTA CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851502.1
CDS	143..6142
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996048.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996048.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGT GGACGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TACGTCAACC TGTACGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGACCC CTACGCGGAG CTCAACACAG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGCC GGCTGCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGAGCAGAAG 
GTGGAGGATC TTCCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTAC AGGACGTGGA CGACGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTACGGG AACAAGTTCG ACACGACCTG CCTGCCTCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACCGA 
GTGGAGGCTC AGAACCAGCT GCTCAGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG 
GTTCACTTGG CCCTGAGGGC GCAGTGCTCC ACCGAGGACG TGGACTCCCT GGCGGCTCAG 
CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTAG GTGACATCCA TGAGACGCCC 
TCTGCTACCC ACCTGGACCA GTACCTGCAC CGGCTGCGTA TGCGTCACCT GAGCCAGATC 
GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC CACAGTGAAC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCAGATG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT GCAGGGCGAC AAGCGGGTGG CCTACCAGCG GGTGCCTGCC 
CACGAGGTCC TCTTCTCCCG GCGGGGCTCC AGCTACTGTG GCAAGAATTG TGGAAAGCTG 
CAGACGATAT TTCTGAAATA TCCGATGGAG GGGATGCCCA GAGCCAGGAT GCCAGTGCAG 
ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC GTGGATGAAA AGGAGTTCAA TCAGTTTGCA 
GAGGGCAAGC TCTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAGCT GGCCCTGGTC 
GGAAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC GGGCAAGATC 
AAGCTCCCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGA CCTGGGCTGG AGACTGGTTC 
GTGTGTCCGG AGAAGACCCT GCTTCATGAC ACTGATGCGG GCCATCTGAG CTTCGTGGAG 
GAGGTGTTTG AGAACCAAAC CCGGCTCCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAATG CCCACTGGGC 
TGGAAGTGGG AAGATGAGGA GTGGTCCACG GACCTCAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCTCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCTGAG 
AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC 
GCCTCACTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGACGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG GAGAAGACGG GACCTGCAGC TGTGTTCGCC 
CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC GACAGGAGTG AGGATTCCAT GTCTGTCTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATCTCCTGCA TCTTCGACTA TGGAAACCGC 
TACCATCTAC GTTGCTATAT GTACCAGGCC CGGGATCTGC CTGCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAATACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTACGAGAT TGAGATCTTC 
GGCGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTACG GCGCAGATGA GTTCATGGGA CGCTGCATCT GTCAGCCGAG TCTGGAACGG 
ATGCCCCGGT TGGCCTGGTT CCCTCTGACT AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCTATCC ACCATATTCC CGGTTTTGAG 
GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CGGACCTGCC CTACCCGCCA 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACAGCCATTG AGATCCTGGC ATGGGGGCTT CGGAACATGA AGAGTTACCA GCTGGCCAGT 
GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCCGGGAGG ATCTCTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGGCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGACGTGAG CTTGCTCAGT 
CCTGGGGAAG ACGTGCTGAT TGACATCGAC GACAAGGAAC CCCTCATCCC CATCCAGCTT 
GCGGATGGTC TGTCGGGCTT GGCCCCCACT AACATGGCGT CTTCTCCATC CAGTCCTCAT 
GAGGAGGAGT TTATTGACTG GTGGAGCAAA TTCTTCGCCT CGATTGGGGA GAGGGAAAAG 
TGTGGGTCCT ACCTGGAGAA GGACTTTGAC ACTCTAAAGG TCTATGATAC CCCGCTGGAG 
AATGTGAAGG CTTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGATGGA AGACCCATCT GTCATTGGGG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCTGCCATT CCCATGCCTC CAAGACAGTT CCACCAGCTG 
GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC CGCATCTACA TCATCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCGGTGAGCG ACCAAGACAA CTACATCCCC TGCACCCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACCCT CTATGACTAC 
GACCTCCTCT CCAAGGATGA AAAGATCGGG GAGACGGTCA TCGACCTGGA GAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC CCCCAGACCT ATTGCGTCTC TGGACCCAAC 
CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG CTTCTCCACC TGTTCTGCCA GCAGCACAGG 
GTCAAGGCCC CCGTGTACCG GACGGACCGC GTGGTGTTTC AGGATAAAGA ATACACCATT 
GAGGAAATAG AGGCTGGCAG GGTCCCAAAC CCACACCTGG GCCCAGCAGA GGAGCGTCTG 
GCCTTGTATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC 
TATAGCCCCC TGCAGCCGGA CATCGAGCAG GGGAAGCTGC AGATGTGGAT CGACCTGTTT 
CCAAAGGCCC TGGGGCGGCC GGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGGAGG 
TTTTTCCTGC GTTGTATTAT CTGGAACACC AAGGATGTGA TTCTGGATGA CCTCAGCATC 
ACCGGGGAGA AGATGAGTGA CATTTATGTG AAAGGCTGGA TGGTGGGCTT TGAAGAACAC 
AAGCAGAAGA CAGATGTGCA TTATCGGTCC CTGGGAGGCG AAGGCAACTT TAACTGGAGG 
TTCGTTTTCC CTTTCGACTA CCTGCCAGCT GAGCAAGTCT GCACCGTCTC CAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAGATCCCTG CGCGAGTAGT CTTCCAGATT 
TGGGACAACG ACAAGTTCTC CTTTGATGAC TTTTTGGGTG AGCACCATTT CTGGTCTGTA 
CCACTCTCCA CTCGGGGCTG CCCCAGAACC CACGCTTTGC GGTGTTGGAT GGTAAGACTC 
AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG CAGTGCAGGC TCCAGTGGGC CGTCACTGGC 
TGGCTGGCCT CTCACTTGAC AGGATCTCTA CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996050.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851504.1 Sus scrofa dysferlin (LOC100522732), transcript variant X13, mRNA.

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CloneID	OSe34212
Clone ID Related Accession (Same CDS sequence)	XM_013996050.1
Accession Version	XM_013996050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGG AGGAGGAGTT TATTGACTGG 
TGGAGCAAAT TCTTCGCCTC GATTGGGGAG AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGATACC CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA 
GACCCATCTG TCATTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC 
CCTGCCATTC CCATGCCTCC AAGACAGTTC CACCAGCTGG CCTCCCAGGA GCCCCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGCGA CCAAGACAAC 
TACATCCCCT GCACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACCCTC TATGACTACG ACCTCCTCTC CAAGGATGAA 
AAGATCGGGG AGACGGTCAT CGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGCCTCC CCCAGACCTA TTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGT 
CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCCAAACC CACACCTGGG CCCAGCAGAG GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAACA CGTGGAGTCC CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGGAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT TCTGGATGAC CTCAGCATCA CCGGGGAGAA GATGAGTGAC 
ATTTATGTGA AAGGCTGGAT GGTGGGCTTT GAAGAACACA AGCAGAAGAC AGATGTGCAT 
TATCGGTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCGTTTTCCC TTTCGACTAC 
CTGCCAGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AGATCCCTGC GCGAGTAGTC TTCCAGATTT GGGACAACGA CAAGTTCTCC 
TTTGATGACT TTTTGGGTGA GCACCATTTC TGGTCTGTAC CACTCTCCAC TCGGGGCTGC 
CCCAGAACCC ACGCTTTGCG GTGTTGGATG GTAAGACTCA GGGTGGTGGT GGGGGGCACA 
GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC GTCACTGGCT GGCTGGCCTC TCACTTGACA 
GGATCTCTAC CACCTCAGTC GAGACAGCTT GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851504.1
CDS	143..6121
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996050.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996050.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGG AGGAGGAGTT TATTGACTGG 
TGGAGCAAAT TCTTCGCCTC GATTGGGGAG AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGATACC CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA 
GACCCATCTG TCATTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC 
CCTGCCATTC CCATGCCTCC AAGACAGTTC CACCAGCTGG CCTCCCAGGA GCCCCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGCGA CCAAGACAAC 
TACATCCCCT GCACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACCCTC TATGACTACG ACCTCCTCTC CAAGGATGAA 
AAGATCGGGG AGACGGTCAT CGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGCCTCC CCCAGACCTA TTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGT 
CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCCAAACC CACACCTGGG CCCAGCAGAG GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAACA CGTGGAGTCC CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGGAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT TCTGGATGAC CTCAGCATCA CCGGGGAGAA GATGAGTGAC 
ATTTATGTGA AAGGCTGGAT GGTGGGCTTT GAAGAACACA AGCAGAAGAC AGATGTGCAT 
TATCGGTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCGTTTTCCC TTTCGACTAC 
CTGCCAGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AGATCCCTGC GCGAGTAGTC TTCCAGATTT GGGACAACGA CAAGTTCTCC 
TTTGATGACT TTTTGGGTGA GCACCATTTC TGGTCTGTAC CACTCTCCAC TCGGGGCTGC 
CCCAGAACCC ACGCTTTGCG GTGTTGGATG GTAAGACTCA GGGTGGTGGT GGGGGGCACA 
GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC GTCACTGGCT GGCTGGCCTC TCACTTGACA 
GGATCTCTAC CACCTCAGTC GAGACAGCTT GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996054.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851508.1 Sus scrofa dysferlin (LOC100522732), transcript variant X17, mRNA.

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CloneID	OSe34216
Clone ID Related Accession (Same CDS sequence)	XM_013996054.1
Accession Version	XM_013996054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGT GGACGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TACGTCAACC TGTACGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGACCC CTACGCGGAG CTCAACACAG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGCC GGCTGCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGAGCAGAAG 
GTGGAGGATC TTCCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTAC AGGACGTGGA CGACGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTACGGG AACAAGTTCG ACACGACCTG CCTGCCTCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACCGA 
GTGGAGGCTC AGAACCAGCT GCTCAGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG 
GTTCACTTGG CCCTGAGGGC GCAGTGCTCC ACCGAGGACG TGGACTCCCT GGCGGCTCAG 
CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTAG GTGACATCCA TGAGACGCCC 
TCTGCTACCC ACCTGGACCA GTACCTGCAC CGGCTGCGTA TGCGTCACCT GAGCCAGATC 
GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC CACAGTGAAC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCAGATG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT GCAGGGCGAC AAGCGGGTGG CCTACCAGCG GGTGCCTGCC 
CACGAGGTCC TCTTCTCCCG GCGGGGCTCC AGCTACTGTG GCAAGAATTG TGGAAAGCTG 
CAGACGATAT TTCTGAAATA TCCGATGGAG GGGATGCCCA GAGCCAGGAT GCCAGTGCAG 
ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC GTGGATGAAA AGGAGTTCAA TCAGTTTGCA 
GAGGGCAAGC TCTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAGCT GGCCCTGGTC 
GGAAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC GGGCAAGATC 
AAGCTCCCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGA CCTGGGCTGG AGACTGGTTC 
GTGTGTCCGG AGAAGACCCT GCTTCATGAC ACTGATGCGG GCCATCTGAG CTTCGTGGAG 
GAGGTGTTTG AGAACCAAAC CCGGCTCCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAATG CCCACTGGGC 
TGGAAGTGGG AAGATGAGGA GTGGTCCACG GACCTCAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCTCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCTGAG 
AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC 
GCCTCACTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGACGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG GAGAAGACGG GACCTGCAGC TGTGTTCGCC 
CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC GACAGGAGTG AGGATTCCAT GTCTGTCTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATCTCCTGCA TCTTCGACTA TGGAAACCGC 
TACCATCTAC GTTGCTATAT GTACCAGGCC CGGGATCTGC CTGCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAATACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTACGAGAT TGAGATCTTC 
GGCGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTACG GCGCAGATGA GTTCATGGGA CGCTGCATCT GTCAGCCGAG TCTGGAACGG 
ATGCCCCGGT TGGCCTGGTT CCCTCTGACT AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCTATCC ACCATATTCC CGGTTTTGAG 
GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CGGACCTGCC CTACCCGCCA 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACAGCCATTG AGATCCTGGC ATGGGGGCTT CGGAACATGA AGAGTTACCA GCTGGCCAGT 
GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCCGGGAGG ATCTCTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGGCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGACGTGAG CTTGCTCAGT 
CCTGGGGAAG ACGTGCTGAT TGACATCGAC GACAAGGAAC CCCTCATCCC CATCCAGGAG 
GAGGAGTTTA TTGACTGGTG GAGCAAATTC TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT 
GGGTCCTACC TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGATACCCC GCTGGAGAAT 
GTGAAGGCTT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGATGGAAGA CCCATCTGTC ATTGGGGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC TGCCATTCCC ATGCCTCCAA GACAGTTCCA CCAGCTGGCC 
TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGCGACC AAGACAACTA CATCCCCTGC ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACCCTCTA TGACTACGAC 
CTCCTCTCCA AGGATGAAAA GATCGGGGAG ACGGTCATCG ACCTGGAGAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC CAGACCTATT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGTCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCCAAACCCA CACCTGGGCC CAGCAGAGGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAACACG TGGAGTCCCG CCCCCTCTAT 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGGAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATTC TGGATGACCT CAGCATCACC 
GGGGAGAAGA TGAGTGACAT TTATGTGAAA GGCTGGATGG TGGGCTTTGA AGAACACAAG 
CAGAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
GTTTTCCCTT TCGACTACCT GCCAGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAG ATCCCTGCGC GAGTAGTCTT CCAGATTTGG 
GACAACGACA AGTTCTCCTT TGATGACTTT TTGGGTGAGC ACCATTTCTG GTCTGTACCA 
CTCTCCACTC GGGGCTGCCC CAGAACCCAC GCTTTGCGGT GTTGGATGGT AAGACTCAGG 
GTGGTGGTGG GGGGCACAGG GTGGAGGCAG TGCAGGCTCC AGTGGGCCGT CACTGGCTGG 
CTGGCCTCTC ACTTGACAGG ATCTCTACCA CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851508.1
CDS	143..6079
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996054.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996054.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGT GGACGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TACGTCAACC TGTACGGCAG CCCCAGGGAG 
TTCACTGGCT TCCCGGACCC CTACGCGGAG CTCAACACAG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGCC GGCTGCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGAGCAGAAG 
GTGGAGGATC TTCCTGCAGA TGACATCCTT CGGGTGGAGA AGTACCTCCG GAGGCGCAAG 
TACTCGCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTAC AGGACGTGGA CGACGCCATC 
CAGTTCGAGG TCAGCATCGG GAACTACGGG AACAAGTTCG ACACGACCTG CCTGCCTCTG 
GCCTCCACCA CCCAGTACAG CCGGGCGGTC TTTGACGGAT GCCACTACTA CTACTTACCC 
TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCATCCTACT GGGAGGACAT CAGCCACCGA 
GTGGAGGCTC AGAACCAGCT GCTCAGGATC GCCGACCGGC TGGAAGCCGG CCTGGAGCAG 
GTTCACTTGG CCCTGAGGGC GCAGTGCTCC ACCGAGGACG TGGACTCCCT GGCGGCTCAG 
CTGATGGATG AGCTCATTGC AGACTGCAGC CAGCCCCTAG GTGACATCCA TGAGACGCCC 
TCTGCTACCC ACCTGGACCA GTACCTGCAC CGGCTGCGTA TGCGTCACCT GAGCCAGATC 
GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC CACAGTGAAC TCCCCGCTGC CCTGGAGCAG 
GCTGAGGACT GGCTCCTGCG TCTCCGTGCC CTGGCAGATG AGCCCCAGAA CAGCCTGCCT 
GACATCGTCA TCTGGATGCT GCAGGGCGAC AAGCGGGTGG CCTACCAGCG GGTGCCTGCC 
CACGAGGTCC TCTTCTCCCG GCGGGGCTCC AGCTACTGTG GCAAGAATTG TGGAAAGCTG 
CAGACGATAT TTCTGAAATA TCCGATGGAG GGGATGCCCA GAGCCAGGAT GCCAGTGCAG 
ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC GTGGATGAAA AGGAGTTCAA TCAGTTTGCA 
GAGGGCAAGC TCTCTGTCTT TGCTGAAACC TATGAGAACC AGACCAAGCT GGCCCTGGTC 
GGAAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAC GGGCAAGATC 
AAGCTCCCCA AGGACAGCTT CCGCCCCTCG GCCGGCTGGA CCTGGGCTGG AGACTGGTTC 
GTGTGTCCGG AGAAGACCCT GCTTCATGAC ACTGATGCGG GCCATCTGAG CTTCGTGGAG 
GAGGTGTTTG AGAACCAAAC CCGGCTCCCC GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACGGATG TGAACGGGGA GAAGGTGCTT CCCAAGGATG ACATCGAATG CCCACTGGGC 
TGGAAGTGGG AAGATGAGGA GTGGTCCACG GACCTCAATC GGGCTGTCGA TGAACAAGGC 
TGGGAGTACA GCATCACCAT CCCTCCGGAC CGGAAGCCGA GGCACTGGGT CCCTGCTGAG 
AAGATGTACT ACACACACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGGAG GGACCTCAGC 
CAGATGGAGG CGCTGAAGAG GCACAGGCAG GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC 
GCCTCACTCT TTGGCTGGAA GTTCCACCTG GAGTACCGCA AGACAGACGC CTTTCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG GAGAAGACGG GACCTGCAGC TGTGTTCGCC 
CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC GACAGGAGTG AGGATTCCAT GTCTGTCTCC 
ACCTTGAGCT TTGGCGTGAA CAGACCCACG ATCTCCTGCA TCTTCGACTA TGGAAACCGC 
TACCATCTAC GTTGCTATAT GTACCAGGCC CGGGATCTGC CTGCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAATACCC TGAACCCCAC CTGGGACCAG ACACTCATCT TCTACGAGAT TGAGATCTTC 
GGCGAGCCAG CCAGCATCGC CGAGCAGCCG CCCAGCATCG TGGTGGAGCT GTATGACCAT 
GACACCTACG GCGCAGATGA GTTCATGGGA CGCTGCATCT GTCAGCCGAG TCTGGAACGG 
ATGCCCCGGT TGGCCTGGTT CCCTCTGACT AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAGAAG CCGGCTATCC ACCATATTCC CGGTTTTGAG 
GTGCAGGATA CATCAGGGAT CCTGGAAGAG TCGGAGGACA CGGACCTGCC CTACCCGCCA 
CCCCAGAGGG AGGCCAACAT CTACATGGTC CCCCAGAACA TCAAGCCGGT TCTCCAGCGA 
ACAGCCATTG AGATCCTGGC ATGGGGGCTT CGGAACATGA AGAGTTACCA GCTGGCCAGT 
GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC GGGGGCCAGA CGGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGCACCC TCTTCATGGA AGTGATGCTG 
CCCCGGGAGG ATCTCTACTG TCCCCCCATC GTGGTCAAGG TCATTGATAA CCGGCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC 
CCTTACTCAG AGGAGAGTCC GTCCCCACAG GGCGGCCCAG ATGACGTGAG CTTGCTCAGT 
CCTGGGGAAG ACGTGCTGAT TGACATCGAC GACAAGGAAC CCCTCATCCC CATCCAGGAG 
GAGGAGTTTA TTGACTGGTG GAGCAAATTC TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT 
GGGTCCTACC TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGATACCCC GCTGGAGAAT 
GTGAAGGCTT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGATGGAAGA CCCATCTGTC ATTGGGGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC TGCCATTCCC ATGCCTCCAA GACAGTTCCA CCAGCTGGCC 
TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGCGACC AAGACAACTA CATCCCCTGC ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACCCTCTA TGACTACGAC 
CTCCTCTCCA AGGATGAAAA GATCGGGGAG ACGGTCATCG ACCTGGAGAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC CAGACCTATT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGTCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCCAAACCCA CACCTGGGCC CAGCAGAGGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAACACG TGGAGTCCCG CCCCCTCTAT 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGGAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATTC TGGATGACCT CAGCATCACC 
GGGGAGAAGA TGAGTGACAT TTATGTGAAA GGCTGGATGG TGGGCTTTGA AGAACACAAG 
CAGAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
GTTTTCCCTT TCGACTACCT GCCAGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAG ATCCCTGCGC GAGTAGTCTT CCAGATTTGG 
GACAACGACA AGTTCTCCTT TGATGACTTT TTGGGTGAGC ACCATTTCTG GTCTGTACCA 
CTCTCCACTC GGGGCTGCCC CAGAACCCAC GCTTTGCGGT GTTGGATGGT AAGACTCAGG 
GTGGTGGTGG GGGGCACAGG GTGGAGGCAG TGCAGGCTCC AGTGGGCCGT CACTGGCTGG 
CTGGCCTCTC ACTTGACAGG ATCTCTACCA CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996055.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851509.1 Sus scrofa dysferlin (LOC100522732), transcript variant X18, mRNA.

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CloneID	OSe34217
Clone ID Related Accession (Same CDS sequence)	XM_013996055.1
Accession Version	XM_013996055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGTGGA CGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTAC GTCAACCTGT ACGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCGGAGCTC AACACAGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGCCGGC TGCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGATCTTC CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CTGCCTTCTA CTCGGCCACC ATGCTACAGG ACGTGGACGA CGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTACGGGAAC AAGTTCGACA CGACCTGCCT GCCTCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACCGAGTG 
GAGGCTCAGA ACCAGCTGCT CAGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTT 
CACTTGGCCC TGAGGGCGCA GTGCTCCACC GAGGACGTGG ACTCCCTGGC GGCTCAGCTG 
ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTAGGTG ACATCCATGA GACGCCCTCT 
GCTACCCACC TGGACCAGTA CCTGCACCGG CTGCGTATGC GTCACCTGAG CCAGATCGTT 
GAGGCGGCCC AGGCCCTGAA GCTTGGCCAC AGTGAACTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTGCA GGGCGACAAG CGGGTGGCCT ACCAGCGGGT GCCTGCCCAC 
GAGGTCCTCT TCTCCCGGCG GGGCTCCAGC TACTGTGGCA AGAATTGTGG AAAGCTGCAG 
ACGATATTTC TGAAATATCC GATGGAGGGG ATGCCCAGAG CCAGGATGCC AGTGCAGATT 
CGGGTCAGGC TGTGGTTCGG GCTCTCCGTG GATGAAAAGG AGTTCAATCA GTTTGCAGAG 
GGCAAGCTCT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAGCTGGC CCTGGTCGGA 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG CAAGATCAAG 
CTCCCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCGGAGA AGACCCTGCT TCATGACACT GATGCGGGCC ATCTGAGCTT CGTGGAGGAG 
GTGTTTGAGA ACCAAACCCG GCTCCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAATGCCC ACTGGGCTGG 
AAGTGGGAAG ATGAGGAGTG GTCCACGGAC CTCAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC TCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCTGAGAAG 
ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCGC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GTGAAGGCTG GGAGTACGCC 
TCACTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGACGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCCCTGGAG AAGACGGGAC CTGCAGCTGT GTTCGCCCTC 
GAGGGGGCCC TGGGCGGTGT GGTGGACGAC AGGAGTGAGG ATTCCATGTC TGTCTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATC TCCTGCATCT TCGACTATGG AAACCGCTAC 
CATCTACGTT GCTATATGTA CCAGGCCCGG GATCTGCCTG CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGCGAAG 
AATACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ACGAGATTGA GATCTTCGGC 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGCG CAGATGAGTT CATGGGACGC TGCATCTGTC AGCCGAGTCT GGAACGGATG 
CCCCGGTTGG CCTGGTTCCC TCTGACTAGG GGCAGCCAGC CGGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCTATCCACC ATATTCCCGG TTTTGAGGTG 
CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACGG ACCTGCCCTA CCCGCCACCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACA 
GCCATTGAGA TCCTGGCATG GGGGCTTCGG AACATGAAGA GTTACCAGCT GGCCAGTGTG 
TCTTCCCCCA GCCTGGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
CGGGAGGATC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG GCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGAGCTTCCT GTGTGACCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ACGTGAGCTT GCTCAGTCCT 
GGGGAAGACG TGCTGATTGA CATCGACGAC AAGGAACCCC TCATCCCCAT CCAGCTTGCG 
GATGGTCTGT CGGGCTTGGC CCCCACTAAC ATGGCGTCTT CTCCATCCAG TCCTCATGAG 
GAGGAGTTTA TTGACTGGTG GAGCAAATTC TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT 
GGGTCCTACC TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGATACCCC GCTGGAGAAT 
GTGAAGGCTT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGATGGAAGA CCCATCTGTC ATTGGGGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC TGCCATTCCC ATGCCTCCAA GACAGTTCCA CCAGCTGGCC 
TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGCGACC AAGACAACTA CATCCCCTGC ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACCCTCTA TGACTACGAC 
CTCCTCTCCA AGGATGAAAA GATCGGGGAG ACGGTCATCG ACCTGGAGAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC CAGACCTATT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGTCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCCAAACCCA CACCTGGGCC CAGCAGAGGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAACACG TGGAGTCCCG CCCCCTCTAT 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGGAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATTC TGGATGACCT CAGCATCACC 
GGGGAGAAGA TGAGTGACAT TTATGTGAAA GGCTGGATGG TGGGCTTTGA AGAACACAAG 
CAGAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
GTTTTCCCTT TCGACTACCT GCCAGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAG ATCCCTGCGC GAGTAGTCTT CCAGATTTGG 
GACAACGACA AGTTCTCCTT TGATGACTTT TTGGGTGAGC ACCATTTCTG GTCTGTACCA 
CTCTCCACTC GGGGCTGCCC CAGAACCCAC GCTTTGCGGT GTTGGATGGT AAGACTCAGG 
GTGGTGGTGG GGGGCACAGG GTGGAGGCAG TGCAGGCTCC AGTGGGCCGT CACTGGCTGG 
CTGGCCTCTC ACTTGACAGG ATCTCTACCA CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851509.1
CDS	526..6522
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996055.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996055.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGTGGA CGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTAC GTCAACCTGT ACGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCGGAGCTC AACACAGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGCCGGC TGCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGATCTTC CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CTGCCTTCTA CTCGGCCACC ATGCTACAGG ACGTGGACGA CGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTACGGGAAC AAGTTCGACA CGACCTGCCT GCCTCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACCGAGTG 
GAGGCTCAGA ACCAGCTGCT CAGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTT 
CACTTGGCCC TGAGGGCGCA GTGCTCCACC GAGGACGTGG ACTCCCTGGC GGCTCAGCTG 
ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTAGGTG ACATCCATGA GACGCCCTCT 
GCTACCCACC TGGACCAGTA CCTGCACCGG CTGCGTATGC GTCACCTGAG CCAGATCGTT 
GAGGCGGCCC AGGCCCTGAA GCTTGGCCAC AGTGAACTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTGCA GGGCGACAAG CGGGTGGCCT ACCAGCGGGT GCCTGCCCAC 
GAGGTCCTCT TCTCCCGGCG GGGCTCCAGC TACTGTGGCA AGAATTGTGG AAAGCTGCAG 
ACGATATTTC TGAAATATCC GATGGAGGGG ATGCCCAGAG CCAGGATGCC AGTGCAGATT 
CGGGTCAGGC TGTGGTTCGG GCTCTCCGTG GATGAAAAGG AGTTCAATCA GTTTGCAGAG 
GGCAAGCTCT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAGCTGGC CCTGGTCGGA 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG CAAGATCAAG 
CTCCCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCGGAGA AGACCCTGCT TCATGACACT GATGCGGGCC ATCTGAGCTT CGTGGAGGAG 
GTGTTTGAGA ACCAAACCCG GCTCCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAATGCCC ACTGGGCTGG 
AAGTGGGAAG ATGAGGAGTG GTCCACGGAC CTCAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC TCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCTGAGAAG 
ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCGC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GTGAAGGCTG GGAGTACGCC 
TCACTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGACGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCCCTGGAG AAGACGGGAC CTGCAGCTGT GTTCGCCCTC 
GAGGGGGCCC TGGGCGGTGT GGTGGACGAC AGGAGTGAGG ATTCCATGTC TGTCTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATC TCCTGCATCT TCGACTATGG AAACCGCTAC 
CATCTACGTT GCTATATGTA CCAGGCCCGG GATCTGCCTG CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGCGAAG 
AATACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ACGAGATTGA GATCTTCGGC 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGCG CAGATGAGTT CATGGGACGC TGCATCTGTC AGCCGAGTCT GGAACGGATG 
CCCCGGTTGG CCTGGTTCCC TCTGACTAGG GGCAGCCAGC CGGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCTATCCACC ATATTCCCGG TTTTGAGGTG 
CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACGG ACCTGCCCTA CCCGCCACCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACA 
GCCATTGAGA TCCTGGCATG GGGGCTTCGG AACATGAAGA GTTACCAGCT GGCCAGTGTG 
TCTTCCCCCA GCCTGGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
CGGGAGGATC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG GCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGAGCTTCCT GTGTGACCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ACGTGAGCTT GCTCAGTCCT 
GGGGAAGACG TGCTGATTGA CATCGACGAC AAGGAACCCC TCATCCCCAT CCAGCTTGCG 
GATGGTCTGT CGGGCTTGGC CCCCACTAAC ATGGCGTCTT CTCCATCCAG TCCTCATGAG 
GAGGAGTTTA TTGACTGGTG GAGCAAATTC TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT 
GGGTCCTACC TGGAGAAGGA CTTTGACACT CTAAAGGTCT ATGATACCCC GCTGGAGAAT 
GTGAAGGCTT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGATGGAAGA CCCATCTGTC ATTGGGGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC TGCCATTCCC ATGCCTCCAA GACAGTTCCA CCAGCTGGCC 
TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCG 
GTGAGCGACC AAGACAACTA CATCCCCTGC ACCCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACCCTCTA TGACTACGAC 
CTCCTCTCCA AGGATGAAAA GATCGGGGAG ACGGTCATCG ACCTGGAGAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC CAGACCTATT GCGTCTCTGG ACCCAACCAG 
TGGAGGGATC AGCTCCGTCC CTCCCAGCTT CTCCACCTGT TCTGCCAGCA GCACAGGGTC 
AAGGCCCCCG TGTACCGGAC GGACCGCGTG GTGTTTCAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAGGGT CCCAAACCCA CACCTGGGCC CAGCAGAGGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAACACG TGGAGTCCCG CCCCCTCTAT 
AGCCCCCTGC AGCCGGACAT CGAGCAGGGG AAGCTGCAGA TGTGGATCGA CCTGTTTCCA 
AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGGAGGTTT 
TTCCTGCGTT GTATTATCTG GAACACCAAG GATGTGATTC TGGATGACCT CAGCATCACC 
GGGGAGAAGA TGAGTGACAT TTATGTGAAA GGCTGGATGG TGGGCTTTGA AGAACACAAG 
CAGAAGACAG ATGTGCATTA TCGGTCCCTG GGAGGCGAAG GCAACTTTAA CTGGAGGTTC 
GTTTTCCCTT TCGACTACCT GCCAGCTGAG CAAGTCTGCA CCGTCTCCAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAG ATCCCTGCGC GAGTAGTCTT CCAGATTTGG 
GACAACGACA AGTTCTCCTT TGATGACTTT TTGGGTGAGC ACCATTTCTG GTCTGTACCA 
CTCTCCACTC GGGGCTGCCC CAGAACCCAC GCTTTGCGGT GTTGGATGGT AAGACTCAGG 
GTGGTGGTGG GGGGCACAGG GTGGAGGCAG TGCAGGCTCC AGTGGGCCGT CACTGGCTGG 
CTGGCCTCTC ACTTGACAGG ATCTCTACCA CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996056.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851510.1 Sus scrofa dysferlin (LOC100522732), transcript variant X19, mRNA.

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CloneID	OSe34218
Clone ID Related Accession (Same CDS sequence)	XM_013996056.1
Accession Version	XM_013996056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGGAGG AGGAGTTTAT TGACTGGTGG 
AGCAAATTCT TCGCCTCGAT TGGGGAGAGG GAAAAGTGTG GGTCCTACCT GGAGAAGGAC 
TTTGACACTC TAAAGGTCTA TGATACCCCG CTGGAGAATG TGAAGGCTTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GATGGAAGAC 
CCATCTGTCA TTGGGGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCT 
GCCATTCCCA TGCCTCCAAG ACAGTTCCAC CAGCTGGCCT CCCAGGAGCC CCAGGAGTGC 
CTGGTCCGCA TCTACATCAT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCGG TGAGCGACCA AGACAACTAC 
ATCCCCTGCA CCCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACCCTCTAT GACTACGACC TCCTCTCCAA GGATGAAAAG 
ATCGGGGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGCCTCCCCC AGACCTATTG CGTCTCTGGA CCCAACCAGT GGAGGGATCA GCTCCGTCCC 
TCCCAGCTTC TCCACCTGTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACG 
GACCGCGTGG TGTTTCAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAGGGTC 
CCAAACCCAC ACCTGGGCCC AGCAGAGGAG CGTCTGGCCT TGTATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAACACGT GGAGTCCCGC CCCCTCTATA GCCCCCTGCA GCCGGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGATCGAC CTGTTTCCAA AGGCCCTGGG GCGGCCGGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGGAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACCAAGG ATGTGATTCT GGATGACCTC AGCATCACCG GGGAGAAGAT GAGTGACATT 
TATGTGAAAG GCTGGATGGT GGGCTTTGAA GAACACAAGC AGAAGACAGA TGTGCATTAT 
CGGTCCCTGG GAGGCGAAGG CAACTTTAAC TGGAGGTTCG TTTTCCCTTT CGACTACCTG 
CCAGCTGAGC AAGTCTGCAC CGTCTCCAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAGA TCCCTGCGCG AGTAGTCTTC CAGATTTGGG ACAACGACAA GTTCTCCTTT 
GATGACTTTT TGGGTGAGCA CCATTTCTGG TCTGTACCAC TCTCCACTCG GGGCTGCCCC 
AGAACCCACG CTTTGCGGTG TTGGATGGTA AGACTCAGGG TGGTGGTGGG GGGCACAGGG 
TGGAGGCAGT GCAGGCTCCA GTGGGCCGTC ACTGGCTGGC TGGCCTCTCA CTTGACAGGA 
TCTCTACCAC CTCAGTCGAG ACAGCTTGGC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851510.1
CDS	526..6501
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996056.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996056.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGGAGG AGGAGTTTAT TGACTGGTGG 
AGCAAATTCT TCGCCTCGAT TGGGGAGAGG GAAAAGTGTG GGTCCTACCT GGAGAAGGAC 
TTTGACACTC TAAAGGTCTA TGATACCCCG CTGGAGAATG TGAAGGCTTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GATGGAAGAC 
CCATCTGTCA TTGGGGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCT 
GCCATTCCCA TGCCTCCAAG ACAGTTCCAC CAGCTGGCCT CCCAGGAGCC CCAGGAGTGC 
CTGGTCCGCA TCTACATCAT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCGG TGAGCGACCA AGACAACTAC 
ATCCCCTGCA CCCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACCCTCTAT GACTACGACC TCCTCTCCAA GGATGAAAAG 
ATCGGGGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGCCTCCCCC AGACCTATTG CGTCTCTGGA CCCAACCAGT GGAGGGATCA GCTCCGTCCC 
TCCCAGCTTC TCCACCTGTT CTGCCAGCAG CACAGGGTCA AGGCCCCCGT GTACCGGACG 
GACCGCGTGG TGTTTCAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAGGGTC 
CCAAACCCAC ACCTGGGCCC AGCAGAGGAG CGTCTGGCCT TGTATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAACACGT GGAGTCCCGC CCCCTCTATA GCCCCCTGCA GCCGGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGATCGAC CTGTTTCCAA AGGCCCTGGG GCGGCCGGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGGAGGTTTT TCCTGCGTTG TATTATCTGG 
AACACCAAGG ATGTGATTCT GGATGACCTC AGCATCACCG GGGAGAAGAT GAGTGACATT 
TATGTGAAAG GCTGGATGGT GGGCTTTGAA GAACACAAGC AGAAGACAGA TGTGCATTAT 
CGGTCCCTGG GAGGCGAAGG CAACTTTAAC TGGAGGTTCG TTTTCCCTTT CGACTACCTG 
CCAGCTGAGC AAGTCTGCAC CGTCTCCAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAGA TCCCTGCGCG AGTAGTCTTC CAGATTTGGG ACAACGACAA GTTCTCCTTT 
GATGACTTTT TGGGTGAGCA CCATTTCTGG TCTGTACCAC TCTCCACTCG GGGCTGCCCC 
AGAACCCACG CTTTGCGGTG TTGGATGGTA AGACTCAGGG TGGTGGTGGG GGGCACAGGG 
TGGAGGCAGT GCAGGCTCCA GTGGGCCGTC ACTGGCTGGC TGGCCTCTCA CTTGACAGGA 
TCTCTACCAC CTCAGTCGAG ACAGCTTGGC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996057.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851511.1 Sus scrofa dysferlin (LOC100522732), transcript variant X20, mRNA.

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CloneID	OSe34219
Clone ID Related Accession (Same CDS sequence)	XM_013996057.1
Accession Version	XM_013996057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGTGGA CGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTAC GTCAACCTGT ACGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCGGAGCTC AACACAGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGCCGGC TGCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGATCTTC CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CTGCCTTCTA CTCGGCCACC ATGCTACAGG ACGTGGACGA CGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTACGGGAAC AAGTTCGACA CGACCTGCCT GCCTCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACCGAGTG 
GAGGCTCAGA ACCAGCTGCT CAGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTT 
CACTTGGCCC TGAGGGCGCA GTGCTCCACC GAGGACGTGG ACTCCCTGGC GGCTCAGCTG 
ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTAGGTG ACATCCATGA GACGCCCTCT 
GCTACCCACC TGGACCAGTA CCTGCACCGG CTGCGTATGC GTCACCTGAG CCAGATCGTT 
GAGGCGGCCC AGGCCCTGAA GCTTGGCCAC AGTGAACTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTGCA GGGCGACAAG CGGGTGGCCT ACCAGCGGGT GCCTGCCCAC 
GAGGTCCTCT TCTCCCGGCG GGGCTCCAGC TACTGTGGCA AGAATTGTGG AAAGCTGCAG 
ACGATATTTC TGAAATATCC GATGGAGGGG ATGCCCAGAG CCAGGATGCC AGTGCAGATT 
CGGGTCAGGC TGTGGTTCGG GCTCTCCGTG GATGAAAAGG AGTTCAATCA GTTTGCAGAG 
GGCAAGCTCT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAGCTGGC CCTGGTCGGA 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG CAAGATCAAG 
CTCCCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCGGAGA AGACCCTGCT TCATGACACT GATGCGGGCC ATCTGAGCTT CGTGGAGGAG 
GTGTTTGAGA ACCAAACCCG GCTCCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAATGCCC ACTGGGCTGG 
AAGTGGGAAG ATGAGGAGTG GTCCACGGAC CTCAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC TCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCTGAGAAG 
ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCGC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GTGAAGGCTG GGAGTACGCC 
TCACTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGACGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCCCTGGAG AAGACGGGAC CTGCAGCTGT GTTCGCCCTC 
GAGGGGGCCC TGGGCGGTGT GGTGGACGAC AGGAGTGAGG ATTCCATGTC TGTCTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATC TCCTGCATCT TCGACTATGG AAACCGCTAC 
CATCTACGTT GCTATATGTA CCAGGCCCGG GATCTGCCTG CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGCGAAG 
AATACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ACGAGATTGA GATCTTCGGC 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGCG CAGATGAGTT CATGGGACGC TGCATCTGTC AGCCGAGTCT GGAACGGATG 
CCCCGGTTGG CCTGGTTCCC TCTGACTAGG GGCAGCCAGC CGGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCTATCCACC ATATTCCCGG TTTTGAGGTG 
CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACGG ACCTGCCCTA CCCGCCACCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACA 
GCCATTGAGA TCCTGGCATG GGGGCTTCGG AACATGAAGA GTTACCAGCT GGCCAGTGTG 
TCTTCCCCCA GCCTGGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
CGGGAGGATC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG GCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGAGCTTCCT GTGTGACCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ACGTGAGCTT GCTCAGTCCT 
GGGGAAGACG TGCTGATTGA CATCGACGAC AAGGAACCCC TCATCCCCAT CCAGGAGGAG 
GAGTTTATTG ACTGGTGGAG CAAATTCTTC GCCTCGATTG GGGAGAGGGA AAAGTGTGGG 
TCCTACCTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ATACCCCGCT GGAGAATGTG 
AAGGCTTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA TGGAAGACCC ATCTGTCATT GGGGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCTGC CATTCCCATG CCTCCAAGAC AGTTCCACCA GCTGGCCTCC 
CAGGAGCCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGCGACCAAG ACAACTACAT CCCCTGCACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CCCTCTATGA CTACGACCTC 
CTCTCCAAGG ATGAAAAGAT CGGGGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG ACCTATTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGTCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCC AAACCCACAC CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAACACGTGG AGTCCCGCCC CCTCTATAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG GAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATTCTGG ATGACCTCAG CATCACCGGG 
GAGAAGATGA GTGACATTTA TGTGAAAGGC TGGATGGTGG GCTTTGAAGA ACACAAGCAG 
AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCGTT 
TTCCCTTTCG ACTACCTGCC AGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAGATC CCTGCGCGAG TAGTCTTCCA GATTTGGGAC 
AACGACAAGT TCTCCTTTGA TGACTTTTTG GGTGAGCACC ATTTCTGGTC TGTACCACTC 
TCCACTCGGG GCTGCCCCAG AACCCACGCT TTGCGGTGTT GGATGGTAAG ACTCAGGGTG 
GTGGTGGGGG GCACAGGGTG GAGGCAGTGC AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG 
GCCTCTCACT TGACAGGATC TCTACCACCT CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851511.1
CDS	526..6459
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996057.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996057.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGTGGA CGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTAC GTCAACCTGT ACGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCGGAGCTC AACACAGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGCCGGC TGCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGATCTTC CTGCAGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAC 
TCGCTCTTTG CTGCCTTCTA CTCGGCCACC ATGCTACAGG ACGTGGACGA CGCCATCCAG 
TTCGAGGTCA GCATCGGGAA CTACGGGAAC AAGTTCGACA CGACCTGCCT GCCTCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGATGCC ACTACTACTA CTTACCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCCTACTGGG AGGACATCAG CCACCGAGTG 
GAGGCTCAGA ACCAGCTGCT CAGGATCGCC GACCGGCTGG AAGCCGGCCT GGAGCAGGTT 
CACTTGGCCC TGAGGGCGCA GTGCTCCACC GAGGACGTGG ACTCCCTGGC GGCTCAGCTG 
ATGGATGAGC TCATTGCAGA CTGCAGCCAG CCCCTAGGTG ACATCCATGA GACGCCCTCT 
GCTACCCACC TGGACCAGTA CCTGCACCGG CTGCGTATGC GTCACCTGAG CCAGATCGTT 
GAGGCGGCCC AGGCCCTGAA GCTTGGCCAC AGTGAACTCC CCGCTGCCCT GGAGCAGGCT 
GAGGACTGGC TCCTGCGTCT CCGTGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCTGAC 
ATCGTCATCT GGATGCTGCA GGGCGACAAG CGGGTGGCCT ACCAGCGGGT GCCTGCCCAC 
GAGGTCCTCT TCTCCCGGCG GGGCTCCAGC TACTGTGGCA AGAATTGTGG AAAGCTGCAG 
ACGATATTTC TGAAATATCC GATGGAGGGG ATGCCCAGAG CCAGGATGCC AGTGCAGATT 
CGGGTCAGGC TGTGGTTCGG GCTCTCCGTG GATGAAAAGG AGTTCAATCA GTTTGCAGAG 
GGCAAGCTCT CTGTCTTTGC TGAAACCTAT GAGAACCAGA CCAAGCTGGC CCTGGTCGGA 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG CAAGATCAAG 
CTCCCCAAGG ACAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCGGAGA AGACCCTGCT TCATGACACT GATGCGGGCC ATCTGAGCTT CGTGGAGGAG 
GTGTTTGAGA ACCAAACCCG GCTCCCCGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ACGGGGAGAA GGTGCTTCCC AAGGATGACA TCGAATGCCC ACTGGGCTGG 
AAGTGGGAAG ATGAGGAGTG GTCCACGGAC CTCAATCGGG CTGTCGATGA ACAAGGCTGG 
GAGTACAGCA TCACCATCCC TCCGGACCGG AAGCCGAGGC ACTGGGTCCC TGCTGAGAAG 
ATGTACTACA CACACCGTCG GCGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCGC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GTGAAGGCTG GGAGTACGCC 
TCACTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CAGACGCCTT TCGCCGCCGC 
CGCTGGCGCC GCCGCATGGA GCCCCTGGAG AAGACGGGAC CTGCAGCTGT GTTCGCCCTC 
GAGGGGGCCC TGGGCGGTGT GGTGGACGAC AGGAGTGAGG ATTCCATGTC TGTCTCCACC 
TTGAGCTTTG GCGTGAACAG ACCCACGATC TCCTGCATCT TCGACTATGG AAACCGCTAC 
CATCTACGTT GCTATATGTA CCAGGCCCGG GATCTGCCTG CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACAGT GGTGGCGAAG 
AATACCCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ACGAGATTGA GATCTTCGGC 
GAGCCAGCCA GCATCGCCGA GCAGCCGCCC AGCATCGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGCG CAGATGAGTT CATGGGACGC TGCATCTGTC AGCCGAGTCT GGAACGGATG 
CCCCGGTTGG CCTGGTTCCC TCTGACTAGG GGCAGCCAGC CGGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCTATCCACC ATATTCCCGG TTTTGAGGTG 
CAGGATACAT CAGGGATCCT GGAAGAGTCG GAGGACACGG ACCTGCCCTA CCCGCCACCC 
CAGAGGGAGG CCAACATCTA CATGGTCCCC CAGAACATCA AGCCGGTTCT CCAGCGAACA 
GCCATTGAGA TCCTGGCATG GGGGCTTCGG AACATGAAGA GTTACCAGCT GGCCAGTGTG 
TCTTCCCCCA GCCTGGTGGT GGAGTGCGGG GGCCAGACGG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGCACCCTCT TCATGGAAGT GATGCTGCCC 
CGGGAGGATC TCTACTGTCC CCCCATCGTG GTCAAGGTCA TTGATAACCG GCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGCTCCCTGG AGAGCTTCCT GTGTGACCCT 
TACTCAGAGG AGAGTCCGTC CCCACAGGGC GGCCCAGATG ACGTGAGCTT GCTCAGTCCT 
GGGGAAGACG TGCTGATTGA CATCGACGAC AAGGAACCCC TCATCCCCAT CCAGGAGGAG 
GAGTTTATTG ACTGGTGGAG CAAATTCTTC GCCTCGATTG GGGAGAGGGA AAAGTGTGGG 
TCCTACCTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ATACCCCGCT GGAGAATGTG 
AAGGCTTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA TGGAAGACCC ATCTGTCATT GGGGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCTGC CATTCCCATG CCTCCAAGAC AGTTCCACCA GCTGGCCTCC 
CAGGAGCCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGCGACCAAG ACAACTACAT CCCCTGCACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CCCTCTATGA CTACGACCTC 
CTCTCCAAGG ATGAAAAGAT CGGGGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG ACCTATTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGTCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCC AAACCCACAC CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAACACGTGG AGTCCCGCCC CCTCTATAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG GAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATTCTGG ATGACCTCAG CATCACCGGG 
GAGAAGATGA GTGACATTTA TGTGAAAGGC TGGATGGTGG GCTTTGAAGA ACACAAGCAG 
AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCGTT 
TTCCCTTTCG ACTACCTGCC AGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAGATC CCTGCGCGAG TAGTCTTCCA GATTTGGGAC 
AACGACAAGT TCTCCTTTGA TGACTTTTTG GGTGAGCACC ATTTCTGGTC TGTACCACTC 
TCCACTCGGG GCTGCCCCAG AACCCACGCT TTGCGGTGTT GGATGGTAAG ACTCAGGGTG 
GTGGTGGGGG GCACAGGGTG GAGGCAGTGC AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG 
GCCTCTCACT TGACAGGATC TCTACCACCT CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996042.2 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851496.2 Sus scrofa dysferlin (DYSF), mRNA.

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CloneID	OSe246348
Clone ID Related Accession (Same CDS sequence)	XM_013996042.2
Accession Version	XM_013996042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	LOW QUALITY PROTEIN: dysferlin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013996042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC NTCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGGAGGAGGA GTTTATTGAC TGGTGGAGCA AATTCTTCGC CTCGATTGGG 
GAGAGGGAAA AGTGTGGGTC CTACCTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAT 
ACCCCGCTGG AGAATGTGAA GGCTTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCATTGG GGAATTTAAG 
GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCTGCCA TTCCCATGCC TCCAAGACAG 
TTCCACCAGC TGGCCTCCCA GGAGCCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGA 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG CGACCAAGAC AACTACATCC CCTGCACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACC 
CTCTATGACT ACGACCTCCT CTCCAAGGAT GAAAAGATCG GGGAGACGGT CATCGACCTG 
GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGCC TCCCCCAGAC CTATTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCCAA ACCCACACCT GGGCCCAGCA 
GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA ACACGTGGAG 
TCCCGCCCCC TCTATAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGGA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATTCTGGAT 
GACCTCAGCA TCACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGGTGGGC 
TTTGAAGAAC ACAAGCAGAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCGTTTT CCCTTTCGAC TACCTGCCAG CTGAGCAAGT CTGCACCGTC 
TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAGATCCC TGCGCGAGTA 
GTCTTCCAGA TTTGGGACAA CGACAAGTTC TCCTTTGATG ACTTTTTGGG CTCTTTGCAG 
CTCGACCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC AGAGCGGTTT GTGTCCCTTT TTGAACAGAA AACAGTGAAG 
GGCTGGTGGC CCTGTGTGGC GGATGAGGGT GAGAAGAAGA TAGTGGCGGG TAAGCTGGAA 
ATGACCTTGG AGATTGTCTC AGAGAACGAG CATGAGGAGC GGCCAGCCGG CCAGGGCCGG 
GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CCCCGTACAA GACAATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGCGCC 
ATCATCCTCT TCATCATTCT CTTCATCCTC CTGCTGTTCC TGGGCATCTT CGTCTACGCC 
TTCCCGAACT ATGCCGCCAT GAAGCTGGTG AAGCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851496.2
CDS	164..6508
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFnullRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPERFVSLFEQKTVKGWWPCVADEGEKKIVAGKLEMTLEIVSENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_013996042.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa dysferlin (DYSF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996042.2

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC NTCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGGAGGAGGA GTTTATTGAC TGGTGGAGCA AATTCTTCGC CTCGATTGGG 
GAGAGGGAAA AGTGTGGGTC CTACCTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAT 
ACCCCGCTGG AGAATGTGAA GGCTTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCATTGG GGAATTTAAG 
GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCTGCCA TTCCCATGCC TCCAAGACAG 
TTCCACCAGC TGGCCTCCCA GGAGCCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGA 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG CGACCAAGAC AACTACATCC CCTGCACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACC 
CTCTATGACT ACGACCTCCT CTCCAAGGAT GAAAAGATCG GGGAGACGGT CATCGACCTG 
GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGCC TCCCCCAGAC CTATTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCCAA ACCCACACCT GGGCCCAGCA 
GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA ACACGTGGAG 
TCCCGCCCCC TCTATAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGGA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATTCTGGAT 
GACCTCAGCA TCACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGGTGGGC 
TTTGAAGAAC ACAAGCAGAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCGTTTT CCCTTTCGAC TACCTGCCAG CTGAGCAAGT CTGCACCGTC 
TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAGATCCC TGCGCGAGTA 
GTCTTCCAGA TTTGGGACAA CGACAAGTTC TCCTTTGATG ACTTTTTGGG CTCTTTGCAG 
CTCGACCTCA ACCGCATGCC CAAGCCTGCC AAGACAGCCG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC AGAGCGGTTT GTGTCCCTTT TTGAACAGAA AACAGTGAAG 
GGCTGGTGGC CCTGTGTGGC GGATGAGGGT GAGAAGAAGA TAGTGGCGGG TAAGCTGGAA 
ATGACCTTGG AGATTGTCTC AGAGAACGAG CATGAGGAGC GGCCAGCCGG CCAGGGCCGG 
GATGAGCCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CCCCGTACAA GACAATGAAG TTCATCCTGT GGCGGCGTTT CCGGTGCGCC 
ATCATCCTCT TCATCATTCT CTTCATCCTC CTGCTGTTCC TGGGCATCTT CGTCTACGCC 
TTCCCGAACT ATGCCGCCAT GAAGCTGGTG AAGCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996038.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851492.1 Sus scrofa dysferlin (LOC100522732), transcript variant X1, mRNA.

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CloneID	OSe34200
Clone ID Related Accession (Same CDS sequence)	XM_013996038.1
Accession Version	XM_013996038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGCTTGCGGA TGGTCTGTCG GGCTTGGCCC CCACTAACAT GGCGTCTTCT 
CCATCCAGTC CTCATAAGAT CCTCCTGGAG GAGGAGTTTA TTGACTGGTG GAGCAAATTC 
TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT GGGTCCTACC TGGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGATACCCC GCTGGAGAAT GTGAAGGCTT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC 
ATTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC TGCCATTCCC 
ATGCCTCCAA GACAGTTCCA CCAGCTGGCC TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGCGACC AAGACAACTA CATCCCCTGC 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACCCTCTA TGACTACGAC CTCCTCTCCA AGGATGAAAA GATCGGGGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC 
CAGACCTATT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGTCC CTCCCAGCTT 
CTCCACCTGT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCCAAACCCA 
CACCTGGGCC CAGCAGAGGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAACACG TGGAGTCCCG CCCCCTCTAT AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGGAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATTC TGGATGACCT CAGCATCACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
GGCTGGATGG TGGGCTTTGA AGAACACAAG CAGAAGACAG ATGTGCATTA TCGGTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC GTTTTCCCTT TCGACTACCT GCCAGCTGAG 
CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAG 
ATCCCTGCGC GAGTAGTCTT CCAGATTTGG GACAACGACA AGTTCTCCTT TGATGACTTT 
TTGGGTGAGC ACCATTTCTG GTCTGTACCA CTCTCCACTC GGGGCTGCCC CAGAACCCAC 
GCTTTGCGGT GTTGGATGGT AAGACTCAGG GTGGTGGTGG GGGGCACAGG GTGGAGGCAG 
TGCAGGCTCC AGTGGGCCGT CACTGGCTGG CTGGCCTCTC ACTTGACAGG ATCTCTACCA 
CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851492.1
CDS	144..6290
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHKILLEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996038.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996038.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGCTTGCGGA TGGTCTGTCG GGCTTGGCCC CCACTAACAT GGCGTCTTCT 
CCATCCAGTC CTCATAAGAT CCTCCTGGAG GAGGAGTTTA TTGACTGGTG GAGCAAATTC 
TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT GGGTCCTACC TGGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGATACCCC GCTGGAGAAT GTGAAGGCTT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC 
ATTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC TGCCATTCCC 
ATGCCTCCAA GACAGTTCCA CCAGCTGGCC TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGCGACC AAGACAACTA CATCCCCTGC 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACCCTCTA TGACTACGAC CTCCTCTCCA AGGATGAAAA GATCGGGGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC 
CAGACCTATT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGTCC CTCCCAGCTT 
CTCCACCTGT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCCAAACCCA 
CACCTGGGCC CAGCAGAGGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAACACG TGGAGTCCCG CCCCCTCTAT AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGGAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATTC TGGATGACCT CAGCATCACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
GGCTGGATGG TGGGCTTTGA AGAACACAAG CAGAAGACAG ATGTGCATTA TCGGTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC GTTTTCCCTT TCGACTACCT GCCAGCTGAG 
CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAG 
ATCCCTGCGC GAGTAGTCTT CCAGATTTGG GACAACGACA AGTTCTCCTT TGATGACTTT 
TTGGGTGAGC ACCATTTCTG GTCTGTACCA CTCTCCACTC GGGGCTGCCC CAGAACCCAC 
GCTTTGCGGT GTTGGATGGT AAGACTCAGG GTGGTGGTGG GGGGCACAGG GTGGAGGCAG 
TGCAGGCTCC AGTGGGCCGT CACTGGCTGG CTGGCCTCTC ACTTGACAGG ATCTCTACCA 
CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996039.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851493.1 Sus scrofa dysferlin (LOC100522732), transcript variant X2, mRNA.

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CloneID	OSe34201
Clone ID Related Accession (Same CDS sequence)	XM_013996039.1
Accession Version	XM_013996039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGCTTGCGGA TGGTCTGTCG GGCTTGGCCC CCACTAACAT GGCGTCTTCT 
CCATCCAGTC CTCATGAGGA GGAGTTTATT GACTGGTGGA GCAAATTCTT CGCCTCGATT 
GGGGAGAGGG AAAAGTGTGG GTCCTACCTG GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GATACCCCGC TGGAGAATGT GAAGGCTTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCAT TGGGGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCTG CCATTCCCAT GCCTCCAAGA 
CAGTTCCACC AGCTGGCCTC CCAGGAGCCC CAGGAGTGCC TGGTCCGCAT CTACATCATC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGCGACCAA GACAACTACA TCCCCTGCAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACCCTCTATG ACTACGACCT CCTCTCCAAG GATGAAAAGA TCGGGGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GCCTCCCCCA GACCTATTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGTCCCT CCCAGCTTCT CCACCTGTTC 
TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC CAAACCCACA CCTGGGCCCA 
GCAGAGGAGC GTCTGGCCTT GTATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAACACGTG 
GAGTCCCGCC CCCTCTATAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GGAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATTCTG 
GATGACCTCA GCATCACCGG GGAGAAGATG AGTGACATTT ATGTGAAAGG CTGGATGGTG 
GGCTTTGAAG AACACAAGCA GAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCGT TTTCCCTTTC GACTACCTGC CAGCTGAGCA AGTCTGCACC 
GTCTCCAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAGAT CCCTGCGCGA 
GTAGTCTTCC AGATTTGGGA CAACGACAAG TTCTCCTTTG ATGACTTTTT GGGTGAGCAC 
CATTTCTGGT CTGTACCACT CTCCACTCGG GGCTGCCCCA GAACCCACGC TTTGCGGTGT 
TGGATGGTAA GACTCAGGGT GGTGGTGGGG GGCACAGGGT GGAGGCAGTG CAGGCTCCAG 
TGGGCCGTCA CTGGCTGGCT GGCCTCTCAC TTGACAGGAT CTCTACCACC TCAGTCGAGA 
CAGCTTGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851493.1
CDS	144..6278
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996039.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996039.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGCTTGCGGA TGGTCTGTCG GGCTTGGCCC CCACTAACAT GGCGTCTTCT 
CCATCCAGTC CTCATGAGGA GGAGTTTATT GACTGGTGGA GCAAATTCTT CGCCTCGATT 
GGGGAGAGGG AAAAGTGTGG GTCCTACCTG GAGAAGGACT TTGACACTCT AAAGGTCTAT 
GATACCCCGC TGGAGAATGT GAAGGCTTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ATGGAAGACC CATCTGTCAT TGGGGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCTG CCATTCCCAT GCCTCCAAGA 
CAGTTCCACC AGCTGGCCTC CCAGGAGCCC CAGGAGTGCC TGGTCCGCAT CTACATCATC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCGGT GAGCGACCAA GACAACTACA TCCCCTGCAC CCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACCCTCTATG ACTACGACCT CCTCTCCAAG GATGAAAAGA TCGGGGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GCCTCCCCCA GACCTATTGC 
GTCTCTGGAC CCAACCAGTG GAGGGATCAG CTCCGTCCCT CCCAGCTTCT CCACCTGTTC 
TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG TACCGGACGG ACCGCGTGGT GTTTCAGGAT 
AAAGAATACA CCATTGAGGA AATAGAGGCT GGCAGGGTCC CAAACCCACA CCTGGGCCCA 
GCAGAGGAGC GTCTGGCCTT GTATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAACACGTG 
GAGTCCCGCC CCCTCTATAG CCCCCTGCAG CCGGACATCG AGCAGGGGAA GCTGCAGATG 
TGGATCGACC TGTTTCCAAA GGCCCTGGGG CGGCCGGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GGAGGTTTTT CCTGCGTTGT ATTATCTGGA ACACCAAGGA TGTGATTCTG 
GATGACCTCA GCATCACCGG GGAGAAGATG AGTGACATTT ATGTGAAAGG CTGGATGGTG 
GGCTTTGAAG AACACAAGCA GAAGACAGAT GTGCATTATC GGTCCCTGGG AGGCGAAGGC 
AACTTTAACT GGAGGTTCGT TTTCCCTTTC GACTACCTGC CAGCTGAGCA AGTCTGCACC 
GTCTCCAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAGAT CCCTGCGCGA 
GTAGTCTTCC AGATTTGGGA CAACGACAAG TTCTCCTTTG ATGACTTTTT GGGTGAGCAC 
CATTTCTGGT CTGTACCACT CTCCACTCGG GGCTGCCCCA GAACCCACGC TTTGCGGTGT 
TGGATGGTAA GACTCAGGGT GGTGGTGGGG GGCACAGGGT GGAGGCAGTG CAGGCTCCAG 
TGGGCCGTCA CTGGCTGGCT GGCCTCTCAC TTGACAGGAT CTCTACCACC TCAGTCGAGA 
CAGCTTGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996040.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851494.1 Sus scrofa dysferlin (LOC100522732), transcript variant X3, mRNA.

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CloneID	OSe34202
Clone ID Related Accession (Same CDS sequence)	XM_013996040.1
Accession Version	XM_013996040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG CTTGCGGATG GTCTGTCGGG CTTGGCCCCC 
ACTAACATGG CGTCTTCTCC ATCCAGTCCT CATAAGATCC TCCTGGAGGA GGAGTTTATT 
GACTGGTGGA GCAAATTCTT CGCCTCGATT GGGGAGAGGG AAAAGTGTGG GTCCTACCTG 
GAGAAGGACT TTGACACTCT AAAGGTCTAT GATACCCCGC TGGAGAATGT GAAGGCTTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ATGGAAGACC CATCTGTCAT TGGGGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCTG CCATTCCCAT GCCTCCAAGA CAGTTCCACC AGCTGGCCTC CCAGGAGCCC 
CAGGAGTGCC TGGTCCGCAT CTACATCATC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCGGT GAGCGACCAA 
GACAACTACA TCCCCTGCAC CCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACCCTCTATG ACTACGACCT CCTCTCCAAG 
GATGAAAAGA TCGGGGAGAC GGTCATCGAC CTGGAGAACA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GCCTCCCCCA GACCTATTGC GTCTCTGGAC CCAACCAGTG GAGGGATCAG 
CTCCGTCCCT CCCAGCTTCT CCACCTGTTC TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG 
TACCGGACGG ACCGCGTGGT GTTTCAGGAT AAAGAATACA CCATTGAGGA AATAGAGGCT 
GGCAGGGTCC CAAACCCACA CCTGGGCCCA GCAGAGGAGC GTCTGGCCTT GTATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAACACGTG GAGTCCCGCC CCCTCTATAG CCCCCTGCAG 
CCGGACATCG AGCAGGGGAA GCTGCAGATG TGGATCGACC TGTTTCCAAA GGCCCTGGGG 
CGGCCGGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GGAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ACACCAAGGA TGTGATTCTG GATGACCTCA GCATCACCGG GGAGAAGATG 
AGTGACATTT ATGTGAAAGG CTGGATGGTG GGCTTTGAAG AACACAAGCA GAAGACAGAT 
GTGCATTATC GGTCCCTGGG AGGCGAAGGC AACTTTAACT GGAGGTTCGT TTTCCCTTTC 
GACTACCTGC CAGCTGAGCA AGTCTGCACC GTCTCCAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAGAT CCCTGCGCGA GTAGTCTTCC AGATTTGGGA CAACGACAAG 
TTCTCCTTTG ATGACTTTTT GGGTGAGCAC CATTTCTGGT CTGTACCACT CTCCACTCGG 
GGCTGCCCCA GAACCCACGC TTTGCGGTGT TGGATGGTAA GACTCAGGGT GGTGGTGGGG 
GGCACAGGGT GGAGGCAGTG CAGGCTCCAG TGGGCCGTCA CTGGCTGGCT GGCCTCTCAC 
TTGACAGGAT CTCTACCACC TCAGTCGAGA CAGCTTGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851494.1
CDS	144..6248
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHKILLEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996040.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996040.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG CTTGCGGATG GTCTGTCGGG CTTGGCCCCC 
ACTAACATGG CGTCTTCTCC ATCCAGTCCT CATAAGATCC TCCTGGAGGA GGAGTTTATT 
GACTGGTGGA GCAAATTCTT CGCCTCGATT GGGGAGAGGG AAAAGTGTGG GTCCTACCTG 
GAGAAGGACT TTGACACTCT AAAGGTCTAT GATACCCCGC TGGAGAATGT GAAGGCTTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ATGGAAGACC CATCTGTCAT TGGGGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCTG CCATTCCCAT GCCTCCAAGA CAGTTCCACC AGCTGGCCTC CCAGGAGCCC 
CAGGAGTGCC TGGTCCGCAT CTACATCATC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCGGT GAGCGACCAA 
GACAACTACA TCCCCTGCAC CCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACCCTCTATG ACTACGACCT CCTCTCCAAG 
GATGAAAAGA TCGGGGAGAC GGTCATCGAC CTGGAGAACA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GCCTCCCCCA GACCTATTGC GTCTCTGGAC CCAACCAGTG GAGGGATCAG 
CTCCGTCCCT CCCAGCTTCT CCACCTGTTC TGCCAGCAGC ACAGGGTCAA GGCCCCCGTG 
TACCGGACGG ACCGCGTGGT GTTTCAGGAT AAAGAATACA CCATTGAGGA AATAGAGGCT 
GGCAGGGTCC CAAACCCACA CCTGGGCCCA GCAGAGGAGC GTCTGGCCTT GTATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAACACGTG GAGTCCCGCC CCCTCTATAG CCCCCTGCAG 
CCGGACATCG AGCAGGGGAA GCTGCAGATG TGGATCGACC TGTTTCCAAA GGCCCTGGGG 
CGGCCGGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GGAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ACACCAAGGA TGTGATTCTG GATGACCTCA GCATCACCGG GGAGAAGATG 
AGTGACATTT ATGTGAAAGG CTGGATGGTG GGCTTTGAAG AACACAAGCA GAAGACAGAT 
GTGCATTATC GGTCCCTGGG AGGCGAAGGC AACTTTAACT GGAGGTTCGT TTTCCCTTTC 
GACTACCTGC CAGCTGAGCA AGTCTGCACC GTCTCCAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAGAT CCCTGCGCGA GTAGTCTTCC AGATTTGGGA CAACGACAAG 
TTCTCCTTTG ATGACTTTTT GGGTGAGCAC CATTTCTGGT CTGTACCACT CTCCACTCGG 
GGCTGCCCCA GAACCCACGC TTTGCGGTGT TGGATGGTAA GACTCAGGGT GGTGGTGGGG 
GGCACAGGGT GGAGGCAGTG CAGGCTCCAG TGGGCCGTCA CTGGCTGGCT GGCCTCTCAC 
TTGACAGGAT CTCTACCACC TCAGTCGAGA CAGCTTGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996041.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851495.1 Sus scrofa dysferlin (LOC100522732), transcript variant X4, mRNA.

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CloneID	OSe34203
Clone ID Related Accession (Same CDS sequence)	XM_013996041.1
Accession Version	XM_013996041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG CTTGCGGATG GTCTGTCGGG CTTGGCCCCC 
ACTAACATGG CGTCTTCTCC ATCCAGTCCT CATGAGGAGG AGTTTATTGA CTGGTGGAGC 
AAATTCTTCG CCTCGATTGG GGAGAGGGAA AAGTGTGGGT CCTACCTGGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA TACCCCGCTG GAGAATGTGA AGGCTTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAT GGAAGACCCA 
TCTGTCATTG GGGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCTGCC 
ATTCCCATGC CTCCAAGACA GTTCCACCAG CTGGCCTCCC AGGAGCCCCA GGAGTGCCTG 
GTCCGCATCT ACATCATCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GCGACCAAGA CAACTACATC 
CCCTGCACCC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC CCTCTATGAC TACGACCTCC TCTCCAAGGA TGAAAAGATC 
GGGGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGC 
CTCCCCCAGA CCTATTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGTCCCTCC 
CAGCTTCTCC ACCTGTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAGGGTCCCA 
AACCCACACC TGGGCCCAGC AGAGGAGCGT CTGGCCTTGT ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AACACGTGGA GTCCCGCCCC CTCTATAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GATCGACCTG TTTCCAAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGG AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATTCTGGA TGACCTCAGC ATCACCGGGG AGAAGATGAG TGACATTTAT 
GTGAAAGGCT GGATGGTGGG CTTTGAAGAA CACAAGCAGA AGACAGATGT GCATTATCGG 
TCCCTGGGAG GCGAAGGCAA CTTTAACTGG AGGTTCGTTT TCCCTTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGCACCGT CTCCAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAGATCC CTGCGCGAGT AGTCTTCCAG ATTTGGGACA ACGACAAGTT CTCCTTTGAT 
GACTTTTTGG GTGAGCACCA TTTCTGGTCT GTACCACTCT CCACTCGGGG CTGCCCCAGA 
ACCCACGCTT TGCGGTGTTG GATGGTAAGA CTCAGGGTGG TGGTGGGGGG CACAGGGTGG 
AGGCAGTGCA GGCTCCAGTG GGCCGTCACT GGCTGGCTGG CCTCTCACTT GACAGGATCT 
CTACCACCTC AGTCGAGACA GCTTGGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851495.1
CDS	143..6235
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996041.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996041.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG CTTGCGGATG GTCTGTCGGG CTTGGCCCCC 
ACTAACATGG CGTCTTCTCC ATCCAGTCCT CATGAGGAGG AGTTTATTGA CTGGTGGAGC 
AAATTCTTCG CCTCGATTGG GGAGAGGGAA AAGTGTGGGT CCTACCTGGA GAAGGACTTT 
GACACTCTAA AGGTCTATGA TACCCCGCTG GAGAATGTGA AGGCTTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAT GGAAGACCCA 
TCTGTCATTG GGGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCTGCC 
ATTCCCATGC CTCCAAGACA GTTCCACCAG CTGGCCTCCC AGGAGCCCCA GGAGTGCCTG 
GTCCGCATCT ACATCATCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCGGTGA GCGACCAAGA CAACTACATC 
CCCTGCACCC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC CCTCTATGAC TACGACCTCC TCTCCAAGGA TGAAAAGATC 
GGGGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGC 
CTCCCCCAGA CCTATTGCGT CTCTGGACCC AACCAGTGGA GGGATCAGCT CCGTCCCTCC 
CAGCTTCTCC ACCTGTTCTG CCAGCAGCAC AGGGTCAAGG CCCCCGTGTA CCGGACGGAC 
CGCGTGGTGT TTCAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAGGGTCCCA 
AACCCACACC TGGGCCCAGC AGAGGAGCGT CTGGCCTTGT ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AACACGTGGA GTCCCGCCCC CTCTATAGCC CCCTGCAGCC GGACATCGAG 
CAGGGGAAGC TGCAGATGTG GATCGACCTG TTTCCAAAGG CCCTGGGGCG GCCGGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGG AGGTTTTTCC TGCGTTGTAT TATCTGGAAC 
ACCAAGGATG TGATTCTGGA TGACCTCAGC ATCACCGGGG AGAAGATGAG TGACATTTAT 
GTGAAAGGCT GGATGGTGGG CTTTGAAGAA CACAAGCAGA AGACAGATGT GCATTATCGG 
TCCCTGGGAG GCGAAGGCAA CTTTAACTGG AGGTTCGTTT TCCCTTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGCACCGT CTCCAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAGATCC CTGCGCGAGT AGTCTTCCAG ATTTGGGACA ACGACAAGTT CTCCTTTGAT 
GACTTTTTGG GTGAGCACCA TTTCTGGTCT GTACCACTCT CCACTCGGGG CTGCCCCAGA 
ACCCACGCTT TGCGGTGTTG GATGGTAAGA CTCAGGGTGG TGGTGGGGGG CACAGGGTGG 
AGGCAGTGCA GGCTCCAGTG GGCCGTCACT GGCTGGCTGG CCTCTCACTT GACAGGATCT 
CTACCACCTC AGTCGAGACA GCTTGGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996042.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851496.1 Sus scrofa dysferlin (LOC100522732), transcript variant X5, mRNA.

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CloneID	OSe34204
Clone ID Related Accession (Same CDS sequence)	XM_013996042.1
Accession Version	XM_013996042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGGAGGAGGA GTTTATTGAC TGGTGGAGCA AATTCTTCGC CTCGATTGGG 
GAGAGGGAAA AGTGTGGGTC CTACCTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAT 
ACCCCGCTGG AGAATGTGAA GGCTTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCATTGG GGAATTTAAG 
GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCTGCCA TTCCCATGCC TCCAAGACAG 
TTCCACCAGC TGGCCTCCCA GGAGCCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGA 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG CGACCAAGAC AACTACATCC CCTGCACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACC 
CTCTATGACT ACGACCTCCT CTCCAAGGAT GAAAAGATCG GGGAGACGGT CATCGACCTG 
GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGCC TCCCCCAGAC CTATTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCCAA ACCCACACCT GGGCCCAGCA 
GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA ACACGTGGAG 
TCCCGCCCCC TCTATAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGGA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATTCTGGAT 
GACCTCAGCA TCACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGGTGGGC 
TTTGAAGAAC ACAAGCAGAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCGTTTT CCCTTTCGAC TACCTGCCAG CTGAGCAAGT CTGCACCGTC 
TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAGATCCC TGCGCGAGTA 
GTCTTCCAGA TTTGGGACAA CGACAAGTTC TCCTTTGATG ACTTTTTGGG TGAGCACCAT 
TTCTGGTCTG TACCACTCTC CACTCGGGGC TGCCCCAGAA CCCACGCTTT GCGGTGTTGG 
ATGGTAAGAC TCAGGGTGGT GGTGGGGGGC ACAGGGTGGA GGCAGTGCAG GCTCCAGTGG 
GCCGTCACTG GCTGGCTGGC CTCTCACTTG ACAGGATCTC TACCACCTCA GTCGAGACAG 
CTTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851496.1
CDS	143..6214
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996042.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996042.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGAGGAGCCT GCAGGTGCGG TCAAGCCTTC TTCAGCCACA 
GACCTGGACG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTACGT CAACCTGTAC 
GGCAGCCCCA GGGAGTTCAC TGGCTTCCCG GACCCCTACG CGGAGCTCAA CACAGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGCCGGCTG CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGAGC AGAAGGTGGA GGATCTTCCT GCAGATGACA TCCTTCGGGT GGAGAAGTAC 
CTCCGGAGGC GCAAGTACTC GCTCTTTGCT GCCTTCTACT CGGCCACCAT GCTACAGGAC 
GTGGACGACG CCATCCAGTT CGAGGTCAGC ATCGGGAACT ACGGGAACAA GTTCGACACG 
ACCTGCCTGC CTCTGGCCTC CACCACCCAG TACAGCCGGG CGGTCTTTGA CGGATGCCAC 
TACTACTACT TACCCTGGGG CAACGTGAAG CCCGTGGTGG TGCTGTCATC CTACTGGGAG 
GACATCAGCC ACCGAGTGGA GGCTCAGAAC CAGCTGCTCA GGATCGCCGA CCGGCTGGAA 
GCCGGCCTGG AGCAGGTTCA CTTGGCCCTG AGGGCGCAGT GCTCCACCGA GGACGTGGAC 
TCCCTGGCGG CTCAGCTGAT GGATGAGCTC ATTGCAGACT GCAGCCAGCC CCTAGGTGAC 
ATCCATGAGA CGCCCTCTGC TACCCACCTG GACCAGTACC TGCACCGGCT GCGTATGCGT 
CACCTGAGCC AGATCGTTGA GGCGGCCCAG GCCCTGAAGC TTGGCCACAG TGAACTCCCC 
GCTGCCCTGG AGCAGGCTGA GGACTGGCTC CTGCGTCTCC GTGCCCTGGC AGATGAGCCC 
CAGAACAGCC TGCCTGACAT CGTCATCTGG ATGCTGCAGG GCGACAAGCG GGTGGCCTAC 
CAGCGGGTGC CTGCCCACGA GGTCCTCTTC TCCCGGCGGG GCTCCAGCTA CTGTGGCAAG 
AATTGTGGAA AGCTGCAGAC GATATTTCTG AAATATCCGA TGGAGGGGAT GCCCAGAGCC 
AGGATGCCAG TGCAGATTCG GGTCAGGCTG TGGTTCGGGC TCTCCGTGGA TGAAAAGGAG 
TTCAATCAGT TTGCAGAGGG CAAGCTCTCT GTCTTTGCTG AAACCTATGA GAACCAGACC 
AAGCTGGCCC TGGTCGGAAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCACGGGCA AGATCAAGCT CCCCAAGGAC AGCTTCCGCC CCTCGGCCGG CTGGACCTGG 
GCTGGAGACT GGTTCGTGTG TCCGGAGAAG ACCCTGCTTC ATGACACTGA TGCGGGCCAT 
CTGAGCTTCG TGGAGGAGGT GTTTGAGAAC CAAACCCGGC TCCCCGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC GGATGTGAAC GGGGAGAAGG TGCTTCCCAA GGATGACATC 
GAATGCCCAC TGGGCTGGAA GTGGGAAGAT GAGGAGTGGT CCACGGACCT CAATCGGGCT 
GTCGATGAAC AAGGCTGGGA GTACAGCATC ACCATCCCTC CGGACCGGAA GCCGAGGCAC 
TGGGTCCCTG CTGAGAAGAT GTACTACACA CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGGAGGGACC TCAGCCAGAT GGAGGCGCTG AAGAGGCACA GGCAGGCAGA GGCGGAGGGT 
GAAGGCTGGG AGTACGCCTC ACTCTTTGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACA 
GACGCCTTTC GCCGCCGCCG CTGGCGCCGC CGCATGGAGC CCCTGGAGAA GACGGGACCT 
GCAGCTGTGT TCGCCCTCGA GGGGGCCCTG GGCGGTGTGG TGGACGACAG GAGTGAGGAT 
TCCATGTCTG TCTCCACCTT GAGCTTTGGC GTGAACAGAC CCACGATCTC CTGCATCTTC 
GACTATGGAA ACCGCTACCA TCTACGTTGC TATATGTACC AGGCCCGGGA TCTGCCTGCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA TACCCTGAAC CCCACCTGGG ACCAGACACT CATCTTCTAC 
GAGATTGAGA TCTTCGGCGA GCCAGCCAGC ATCGCCGAGC AGCCGCCCAG CATCGTGGTG 
GAGCTGTATG ACCATGACAC CTACGGCGCA GATGAGTTCA TGGGACGCTG CATCTGTCAG 
CCGAGTCTGG AACGGATGCC CCGGTTGGCC TGGTTCCCTC TGACTAGGGG CAGCCAGCCG 
GCGGGTGAGC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AGAAGCCGGC TATCCACCAT 
ATTCCCGGTT TTGAGGTGCA GGATACATCA GGGATCCTGG AAGAGTCGGA GGACACGGAC 
CTGCCCTACC CGCCACCCCA GAGGGAGGCC AACATCTACA TGGTCCCCCA GAACATCAAG 
CCGGTTCTCC AGCGAACAGC CATTGAGATC CTGGCATGGG GGCTTCGGAA CATGAAGAGT 
TACCAGCTGG CCAGTGTGTC TTCCCCCAGC CTGGTGGTGG AGTGCGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCCG GGAGGATCTC TACTGTCCCC CCATCGTGGT CAAGGTCATT 
GATAACCGGC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCTTA CTCAGAGGAG AGTCCGTCCC CACAGGGCGG CCCAGATGAC 
GTGAGCTTGC TCAGTCCTGG GGAAGACGTG CTGATTGACA TCGACGACAA GGAACCCCTC 
ATCCCCATCC AGGAGGAGGA GTTTATTGAC TGGTGGAGCA AATTCTTCGC CTCGATTGGG 
GAGAGGGAAA AGTGTGGGTC CTACCTGGAG AAGGACTTTG ACACTCTAAA GGTCTATGAT 
ACCCCGCTGG AGAATGTGAA GGCTTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGATG GAAGACCCAT CTGTCATTGG GGAATTTAAG 
GGCCTCTTCA AAATTTATCC CCTCCCAGAA GACCCTGCCA TTCCCATGCC TCCAAGACAG 
TTCCACCAGC TGGCCTCCCA GGAGCCCCAG GAGTGCCTGG TCCGCATCTA CATCATCCGA 
GCATTTGGCC TGCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCGGTGAG CGACCAAGAC AACTACATCC CCTGCACCCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACC 
CTCTATGACT ACGACCTCCT CTCCAAGGAT GAAAAGATCG GGGAGACGGT CATCGACCTG 
GAGAACAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGCC TCCCCCAGAC CTATTGCGTC 
TCTGGACCCA ACCAGTGGAG GGATCAGCTC CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC 
CAGCAGCACA GGGTCAAGGC CCCCGTGTAC CGGACGGACC GCGTGGTGTT TCAGGATAAA 
GAATACACCA TTGAGGAAAT AGAGGCTGGC AGGGTCCCAA ACCCACACCT GGGCCCAGCA 
GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA ACACGTGGAG 
TCCCGCCCCC TCTATAGCCC CCTGCAGCCG GACATCGAGC AGGGGAAGCT GCAGATGTGG 
ATCGACCTGT TTCCAAAGGC CCTGGGGCGG CCGGGACCTC CCTTCAACAT CACCCCACGG 
AGAGCCAGGA GGTTTTTCCT GCGTTGTATT ATCTGGAACA CCAAGGATGT GATTCTGGAT 
GACCTCAGCA TCACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGGTGGGC 
TTTGAAGAAC ACAAGCAGAA GACAGATGTG CATTATCGGT CCCTGGGAGG CGAAGGCAAC 
TTTAACTGGA GGTTCGTTTT CCCTTTCGAC TACCTGCCAG CTGAGCAAGT CTGCACCGTC 
TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAGATCCC TGCGCGAGTA 
GTCTTCCAGA TTTGGGACAA CGACAAGTTC TCCTTTGATG ACTTTTTGGG TGAGCACCAT 
TTCTGGTCTG TACCACTCTC CACTCGGGGC TGCCCCAGAA CCCACGCTTT GCGGTGTTGG 
ATGGTAAGAC TCAGGGTGGT GGTGGGGGGC ACAGGGTGGA GGCAGTGCAG GCTCCAGTGG 
GCCGTCACTG GCTGGCTGGC CTCTCACTTG ACAGGATCTC TACCACCTCA GTCGAGACAG 
CTTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996043.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851497.1 Sus scrofa dysferlin (LOC100522732), transcript variant X6, mRNA.

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CloneID	OSe34205
Clone ID Related Accession (Same CDS sequence)	XM_013996043.1
Accession Version	XM_013996043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA GGTGCGGTCA AGCCTTCTTC AGCCACAGAC 
CTGGACGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTACGTCAA CCTGTACGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCGG AGCTCAACAC AGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG CCGGCTGCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCTGCC TTCTACTCGG CCACCATGCT ACAGGACGTG 
GACGACGCCA TCCAGTTCGA GGTCAGCATC GGGAACTACG GGAACAAGTT CGACACGACC 
TGCCTGCCTC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACC GAGTGGAGGC TCAGAACCAG CTGCTCAGGA TCGCCGACCG GCTGGAAGCC 
GGCCTGGAGC AGGTTCACTT GGCCCTGAGG GCGCAGTGCT CCACCGAGGA CGTGGACTCC 
CTGGCGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT AGGTGACATC 
CATGAGACGC CCTCTGCTAC CCACCTGGAC CAGTACCTGC ACCGGCTGCG TATGCGTCAC 
CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC CTGAAGCTTG GCCACAGTGA ACTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTGCAGGGCG ACAAGCGGGT GGCCTACCAG 
CGGGTGCCTG CCCACGAGGT CCTCTTCTCC CGGCGGGGCT CCAGCTACTG TGGCAAGAAT 
TGTGGAAAGC TGCAGACGAT ATTTCTGAAA TATCCGATGG AGGGGATGCC CAGAGCCAGG 
ATGCCAGTGC AGATTCGGGT CAGGCTGTGG TTCGGGCTCT CCGTGGATGA AAAGGAGTTC 
AATCAGTTTG CAGAGGGCAA GCTCTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAG 
CTGGCCCTGG TCGGAAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGCAAGA TCAAGCTCCC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC GGAGAAGACC CTGCTTCATG ACACTGATGC GGGCCATCTG 
AGCTTCGTGG AGGAGGTGTT TGAGAACCAA ACCCGGCTCC CCGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAA 
TGCCCACTGG GCTGGAAGTG GGAAGATGAG GAGTGGTCCA CGGACCTCAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCTG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGTGAA 
GGCTGGGAGT ACGCCTCACT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAC 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCCC TGGAGAAGAC GGGACCTGCA 
GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC GGTGTGGTGG ACGACAGGAG TGAGGATTCC 
ATGTCTGTCT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATCTCCTG CATCTTCGAC 
TATGGAAACC GCTACCATCT ACGTTGCTAT ATGTACCAGG CCCGGGATCT GCCTGCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAATAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTACGAG 
ATTGAGATCT TCGGCGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGCGCAGAT GAGTTCATGG GACGCTGCAT CTGTCAGCCG 
AGTCTGGAAC GGATGCCCCG GTTGGCCTGG TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCTAT CCACCATATT 
CCCGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCCTACCCGC CACCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACAGCCAT TGAGATCCTG GCATGGGGGC TTCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCCGGGA GGATCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGGCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGAGC 
TTCCTGTGTG ACCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGACGTG 
AGCTTGCTCA GTCCTGGGGA AGACGTGCTG ATTGACATCG ACGACAAGGA ACCCCTCATC 
CCCATCCAGC TTGCGGATGG TCTGTCGGGC TTGGCCCCCA CTAACATGGC GTCTTCTCCA 
TCCAGTCCTC ATAAGATCCT CCTGGAGGAG GAGTTTATTG ACTGGTGGAG CAAATTCTTC 
GCCTCGATTG GGGAGAGGGA AAAGTGTGGG TCCTACCTGG AGAAGGACTT TGACACTCTA 
AAGGTCTATG ATACCCCGCT GGAGAATGTG AAGGCTTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA TGGAAGACCC ATCTGTCATT 
GGGGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCTGC CATTCCCATG 
CCTCCAAGAC AGTTCCACCA GCTGGCCTCC CAGGAGCCCC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCGGTG AGCGACCAAG ACAACTACAT CCCCTGCACC 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CCCTCTATGA CTACGACCTC CTCTCCAAGG ATGAAAAGAT CGGGGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG 
ACCTATTGCG TCTCTGGACC CAACCAGTGG AGGGATCAGC TCCGTCCCTC CCAGCTTCTC 
CACCTGTTCT GCCAGCAGCA CAGGGTCAAG GCCCCCGTGT ACCGGACGGA CCGCGTGGTG 
TTTCAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAGGGTCCC AAACCCACAC 
CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAACACGTGG AGTCCCGCCC CCTCTATAGC CCCCTGCAGC CGGACATCGA GCAGGGGAAG 
CTGCAGATGT GGATCGACCT GTTTCCAAAG GCCCTGGGGC GGCCGGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG GAGGTTTTTC CTGCGTTGTA TTATCTGGAA CACCAAGGAT 
GTGATTCTGG ATGACCTCAG CATCACCGGG GAGAAGATGA GTGACATTTA TGTGAAAGGC 
TGGATGGTGG GCTTTGAAGA ACACAAGCAG AAGACAGATG TGCATTATCG GTCCCTGGGA 
GGCGAAGGCA ACTTTAACTG GAGGTTCGTT TTCCCTTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGCACCG TCTCCAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAGATC 
CCTGCGCGAG TAGTCTTCCA GATTTGGGAC AACGACAAGT TCTCCTTTGA TGACTTTTTG 
GGTGAGCACC ATTTCTGGTC TGTACCACTC TCCACTCGGG GCTGCCCCAG AACCCACGCT 
TTGCGGTGTT GGATGGTAAG ACTCAGGGTG GTGGTGGGGG GCACAGGGTG GAGGCAGTGC 
AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG GCCTCTCACT TGACAGGATC TCTACCACCT 
CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851497.1
CDS	527..6670
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHKILLEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996043.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996043.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA GGTGCGGTCA AGCCTTCTTC AGCCACAGAC 
CTGGACGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTACGTCAA CCTGTACGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCGG AGCTCAACAC AGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG CCGGCTGCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCTGCC TTCTACTCGG CCACCATGCT ACAGGACGTG 
GACGACGCCA TCCAGTTCGA GGTCAGCATC GGGAACTACG GGAACAAGTT CGACACGACC 
TGCCTGCCTC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACC GAGTGGAGGC TCAGAACCAG CTGCTCAGGA TCGCCGACCG GCTGGAAGCC 
GGCCTGGAGC AGGTTCACTT GGCCCTGAGG GCGCAGTGCT CCACCGAGGA CGTGGACTCC 
CTGGCGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT AGGTGACATC 
CATGAGACGC CCTCTGCTAC CCACCTGGAC CAGTACCTGC ACCGGCTGCG TATGCGTCAC 
CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC CTGAAGCTTG GCCACAGTGA ACTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTGCAGGGCG ACAAGCGGGT GGCCTACCAG 
CGGGTGCCTG CCCACGAGGT CCTCTTCTCC CGGCGGGGCT CCAGCTACTG TGGCAAGAAT 
TGTGGAAAGC TGCAGACGAT ATTTCTGAAA TATCCGATGG AGGGGATGCC CAGAGCCAGG 
ATGCCAGTGC AGATTCGGGT CAGGCTGTGG TTCGGGCTCT CCGTGGATGA AAAGGAGTTC 
AATCAGTTTG CAGAGGGCAA GCTCTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAG 
CTGGCCCTGG TCGGAAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGCAAGA TCAAGCTCCC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC GGAGAAGACC CTGCTTCATG ACACTGATGC GGGCCATCTG 
AGCTTCGTGG AGGAGGTGTT TGAGAACCAA ACCCGGCTCC CCGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAA 
TGCCCACTGG GCTGGAAGTG GGAAGATGAG GAGTGGTCCA CGGACCTCAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCTG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGTGAA 
GGCTGGGAGT ACGCCTCACT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAC 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCCC TGGAGAAGAC GGGACCTGCA 
GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC GGTGTGGTGG ACGACAGGAG TGAGGATTCC 
ATGTCTGTCT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATCTCCTG CATCTTCGAC 
TATGGAAACC GCTACCATCT ACGTTGCTAT ATGTACCAGG CCCGGGATCT GCCTGCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAATAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTACGAG 
ATTGAGATCT TCGGCGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGCGCAGAT GAGTTCATGG GACGCTGCAT CTGTCAGCCG 
AGTCTGGAAC GGATGCCCCG GTTGGCCTGG TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCTAT CCACCATATT 
CCCGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCCTACCCGC CACCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACAGCCAT TGAGATCCTG GCATGGGGGC TTCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCCGGGA GGATCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGGCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGAGC 
TTCCTGTGTG ACCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGACGTG 
AGCTTGCTCA GTCCTGGGGA AGACGTGCTG ATTGACATCG ACGACAAGGA ACCCCTCATC 
CCCATCCAGC TTGCGGATGG TCTGTCGGGC TTGGCCCCCA CTAACATGGC GTCTTCTCCA 
TCCAGTCCTC ATAAGATCCT CCTGGAGGAG GAGTTTATTG ACTGGTGGAG CAAATTCTTC 
GCCTCGATTG GGGAGAGGGA AAAGTGTGGG TCCTACCTGG AGAAGGACTT TGACACTCTA 
AAGGTCTATG ATACCCCGCT GGAGAATGTG AAGGCTTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA TGGAAGACCC ATCTGTCATT 
GGGGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCTGC CATTCCCATG 
CCTCCAAGAC AGTTCCACCA GCTGGCCTCC CAGGAGCCCC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCGGTG AGCGACCAAG ACAACTACAT CCCCTGCACC 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CCCTCTATGA CTACGACCTC CTCTCCAAGG ATGAAAAGAT CGGGGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG 
ACCTATTGCG TCTCTGGACC CAACCAGTGG AGGGATCAGC TCCGTCCCTC CCAGCTTCTC 
CACCTGTTCT GCCAGCAGCA CAGGGTCAAG GCCCCCGTGT ACCGGACGGA CCGCGTGGTG 
TTTCAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAGGGTCCC AAACCCACAC 
CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAACACGTGG AGTCCCGCCC CCTCTATAGC CCCCTGCAGC CGGACATCGA GCAGGGGAAG 
CTGCAGATGT GGATCGACCT GTTTCCAAAG GCCCTGGGGC GGCCGGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG GAGGTTTTTC CTGCGTTGTA TTATCTGGAA CACCAAGGAT 
GTGATTCTGG ATGACCTCAG CATCACCGGG GAGAAGATGA GTGACATTTA TGTGAAAGGC 
TGGATGGTGG GCTTTGAAGA ACACAAGCAG AAGACAGATG TGCATTATCG GTCCCTGGGA 
GGCGAAGGCA ACTTTAACTG GAGGTTCGTT TTCCCTTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGCACCG TCTCCAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAGATC 
CCTGCGCGAG TAGTCTTCCA GATTTGGGAC AACGACAAGT TCTCCTTTGA TGACTTTTTG 
GGTGAGCACC ATTTCTGGTC TGTACCACTC TCCACTCGGG GCTGCCCCAG AACCCACGCT 
TTGCGGTGTT GGATGGTAAG ACTCAGGGTG GTGGTGGGGG GCACAGGGTG GAGGCAGTGC 
AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG GCCTCTCACT TGACAGGATC TCTACCACCT 
CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996044.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851498.1 Sus scrofa dysferlin (LOC100522732), transcript variant X7, mRNA.

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CloneID	OSe34206
Clone ID Related Accession (Same CDS sequence)	XM_013996044.1
Accession Version	XM_013996044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGC TTGCGGATGG TCTGTCGGGC 
TTGGCCCCCA CTAACATGGC GTCTTCTCCA TCCAGTCCTC ATAAGATCCT CCTGGAGGAG 
GAGTTTATTG ACTGGTGGAG CAAATTCTTC GCCTCGATTG GGGAGAGGGA AAAGTGTGGG 
TCCTACCTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ATACCCCGCT GGAGAATGTG 
AAGGCTTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA TGGAAGACCC ATCTGTCATT GGGGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCTGC CATTCCCATG CCTCCAAGAC AGTTCCACCA GCTGGCCTCC 
CAGGAGCCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGCGACCAAG ACAACTACAT CCCCTGCACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CCCTCTATGA CTACGACCTC 
CTCTCCAAGG ATGAAAAGAT CGGGGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG ACCTATTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGTCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCC AAACCCACAC CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAACACGTGG AGTCCCGCCC CCTCTATAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG GAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATTCTGG ATGACCTCAG CATCACCGGG 
GAGAAGATGA GTGACATTTA TGTGAAAGGC TGGATGGTGG GCTTTGAAGA ACACAAGCAG 
AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCGTT 
TTCCCTTTCG ACTACCTGCC AGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAGATC CCTGCGCGAG TAGTCTTCCA GATTTGGGAC 
AACGACAAGT TCTCCTTTGA TGACTTTTTG GGTGAGCACC ATTTCTGGTC TGTACCACTC 
TCCACTCGGG GCTGCCCCAG AACCCACGCT TTGCGGTGTT GGATGGTAAG ACTCAGGGTG 
GTGGTGGGGG GCACAGGGTG GAGGCAGTGC AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG 
GCCTCTCACT TGACAGGATC TCTACCACCT CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851498.1
CDS	144..6197
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHKILLEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996044.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996044.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGC TTGCGGATGG TCTGTCGGGC 
TTGGCCCCCA CTAACATGGC GTCTTCTCCA TCCAGTCCTC ATAAGATCCT CCTGGAGGAG 
GAGTTTATTG ACTGGTGGAG CAAATTCTTC GCCTCGATTG GGGAGAGGGA AAAGTGTGGG 
TCCTACCTGG AGAAGGACTT TGACACTCTA AAGGTCTATG ATACCCCGCT GGAGAATGTG 
AAGGCTTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA TGGAAGACCC ATCTGTCATT GGGGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCTGC CATTCCCATG CCTCCAAGAC AGTTCCACCA GCTGGCCTCC 
CAGGAGCCCC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCGGTG 
AGCGACCAAG ACAACTACAT CCCCTGCACC CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CCCTCTATGA CTACGACCTC 
CTCTCCAAGG ATGAAAAGAT CGGGGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGCGG CCTCCCCCAG ACCTATTGCG TCTCTGGACC CAACCAGTGG 
AGGGATCAGC TCCGTCCCTC CCAGCTTCTC CACCTGTTCT GCCAGCAGCA CAGGGTCAAG 
GCCCCCGTGT ACCGGACGGA CCGCGTGGTG TTTCAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAGGGTCCC AAACCCACAC CTGGGCCCAG CAGAGGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAACACGTGG AGTCCCGCCC CCTCTATAGC 
CCCCTGCAGC CGGACATCGA GCAGGGGAAG CTGCAGATGT GGATCGACCT GTTTCCAAAG 
GCCCTGGGGC GGCCGGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG GAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA CACCAAGGAT GTGATTCTGG ATGACCTCAG CATCACCGGG 
GAGAAGATGA GTGACATTTA TGTGAAAGGC TGGATGGTGG GCTTTGAAGA ACACAAGCAG 
AAGACAGATG TGCATTATCG GTCCCTGGGA GGCGAAGGCA ACTTTAACTG GAGGTTCGTT 
TTCCCTTTCG ACTACCTGCC AGCTGAGCAA GTCTGCACCG TCTCCAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAGATC CCTGCGCGAG TAGTCTTCCA GATTTGGGAC 
AACGACAAGT TCTCCTTTGA TGACTTTTTG GGTGAGCACC ATTTCTGGTC TGTACCACTC 
TCCACTCGGG GCTGCCCCAG AACCCACGCT TTGCGGTGTT GGATGGTAAG ACTCAGGGTG 
GTGGTGGGGG GCACAGGGTG GAGGCAGTGC AGGCTCCAGT GGGCCGTCAC TGGCTGGCTG 
GCCTCTCACT TGACAGGATC TCTACCACCT CAGTCGAGAC AGCTTGGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996045.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851499.1 Sus scrofa dysferlin (LOC100522732), transcript variant X8, mRNA.

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CloneID	OSe34207
Clone ID Related Accession (Same CDS sequence)	XM_013996045.1
Accession Version	XM_013996045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGC TTGCGGATGG TCTGTCGGGC 
TTGGCCCCCA CTAACATGGC GTCTTCTCCA TCCAGTCCTC ATGAGGAGGA GTTTATTGAC 
TGGTGGAGCA AATTCTTCGC CTCGATTGGG GAGAGGGAAA AGTGTGGGTC CTACCTGGAG 
AAGGACTTTG ACACTCTAAA GGTCTATGAT ACCCCGCTGG AGAATGTGAA GGCTTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGATG 
GAAGACCCAT CTGTCATTGG GGAATTTAAG GGCCTCTTCA AAATTTATCC CCTCCCAGAA 
GACCCTGCCA TTCCCATGCC TCCAAGACAG TTCCACCAGC TGGCCTCCCA GGAGCCCCAG 
GAGTGCCTGG TCCGCATCTA CATCATCCGA GCATTTGGCC TGCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCGGTGAG CGACCAAGAC 
AACTACATCC CCTGCACCCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACC CTCTATGACT ACGACCTCCT CTCCAAGGAT 
GAAAAGATCG GGGAGACGGT CATCGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGCC TCCCCCAGAC CTATTGCGTC TCTGGACCCA ACCAGTGGAG GGATCAGCTC 
CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC CAGCAGCACA GGGTCAAGGC CCCCGTGTAC 
CGGACGGACC GCGTGGTGTT TCAGGATAAA GAATACACCA TTGAGGAAAT AGAGGCTGGC 
AGGGTCCCAA ACCCACACCT GGGCCCAGCA GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA ACACGTGGAG TCCCGCCCCC TCTATAGCCC CCTGCAGCCG 
GACATCGAGC AGGGGAAGCT GCAGATGTGG ATCGACCTGT TTCCAAAGGC CCTGGGGCGG 
CCGGGACCTC CCTTCAACAT CACCCCACGG AGAGCCAGGA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CCAAGGATGT GATTCTGGAT GACCTCAGCA TCACCGGGGA GAAGATGAGT 
GACATTTATG TGAAAGGCTG GATGGTGGGC TTTGAAGAAC ACAAGCAGAA GACAGATGTG 
CATTATCGGT CCCTGGGAGG CGAAGGCAAC TTTAACTGGA GGTTCGTTTT CCCTTTCGAC 
TACCTGCCAG CTGAGCAAGT CTGCACCGTC TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAGATCCC TGCGCGAGTA GTCTTCCAGA TTTGGGACAA CGACAAGTTC 
TCCTTTGATG ACTTTTTGGG TGAGCACCAT TTCTGGTCTG TACCACTCTC CACTCGGGGC 
TGCCCCAGAA CCCACGCTTT GCGGTGTTGG ATGGTAAGAC TCAGGGTGGT GGTGGGGGGC 
ACAGGGTGGA GGCAGTGCAG GCTCCAGTGG GCCGTCACTG GCTGGCTGGC CTCTCACTTG 
ACAGGATCTC TACCACCTCA GTCGAGACAG CTTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851499.1
CDS	143..6184
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996045.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996045.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG ACACAGGAGG AGAGGAGGAC 
ACGGAGGACC AGGGGCTGAC GGGAGACGAG GCCGAGCCAT TCCCGGACCA GAGCGGAACT 
CCGGGTCCAG GGGCCCCCAT CACCCCAAAG AAGCCACCTT CCCATCCTCC CCACCACCGA 
CCAGGGGGGA GAAGGAGGAG AAGCACGCCC GCGCCCAGAA AGCCGCTGTC GGACAAACCT 
CAGGACTTCC AGATCAGGGT CCAGGTGATC GAGGGACGCC AGCTGCCGGG GGTGAACATC 
AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG CAGACCAAGC GGACTCGGAT CCACAAGGGA 
AACAGCCCCC TCTTCAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TGCGGAGCTG 
TTTAATGAGC CCATCTTCAT CACGGTGGTG GACTCCCGTT CCCTCAGGAC AGACGCTCTC 
ATCGGGGAGT TCCGGATGGA CGTGGGCAGC ATCTACCGGG AACCCCGACA CGCCTATCTC 
AGGAAGTGGC TGCTGCTCTC GGACCCAGAC GACTTCTCTG CTGGGGCCAG AGGCTACCTG 
AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA GACGAGGCCC CTCTGGAGAG GAAAGACCCC 
TCTGAGGACA AGGAGGACAT CGAAGGCAAC CTGCTCAGGC CGACGGGCGT GGCCCTCCGT 
GGAGCGAACT TCTGCCTGAA GGTCTTCAGG GCGGAGGACT TACCGCAGAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGG TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTATGTG GCAAGATCCT GGAGAAGACG 
GCCAACCCTC AGTGGAACCA GAGTATCACG CTGCCTGCCA TGTTTCCCTC CATGTGTGAG 
AAGATGAGGA TTCGTGTCAT GGACTGGGAC CGCCTCACCC ACAACGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA 
GGTGCGGTCA AGCCTTCTTC AGCCACAGAC CTGGACGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTACGTCAA CCTGTACGGC AGCCCCAGGG AGTTCACTGG CTTCCCGGAC 
CCCTACGCGG AGCTCAACAC AGGCAAGGGG GAAGGCGTGG CCTACCGAGG CCGGCTGCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA 
GATGACATCC TTCGGGTGGA GAAGTACCTC CGGAGGCGCA AGTACTCGCT CTTTGCTGCC 
TTCTACTCGG CCACCATGCT ACAGGACGTG GACGACGCCA TCCAGTTCGA GGTCAGCATC 
GGGAACTACG GGAACAAGTT CGACACGACC TGCCTGCCTC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCGG TCTTTGACGG ATGCCACTAC TACTACTTAC CCTGGGGCAA CGTGAAGCCC 
GTGGTGGTGC TGTCATCCTA CTGGGAGGAC ATCAGCCACC GAGTGGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCCGACCG GCTGGAAGCC GGCCTGGAGC AGGTTCACTT GGCCCTGAGG 
GCGCAGTGCT CCACCGAGGA CGTGGACTCC CTGGCGGCTC AGCTGATGGA TGAGCTCATT 
GCAGACTGCA GCCAGCCCCT AGGTGACATC CATGAGACGC CCTCTGCTAC CCACCTGGAC 
CAGTACCTGC ACCGGCTGCG TATGCGTCAC CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC 
CTGAAGCTTG GCCACAGTGA ACTCCCCGCT GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG AACAGCCTGC CTGACATCGT CATCTGGATG 
CTGCAGGGCG ACAAGCGGGT GGCCTACCAG CGGGTGCCTG CCCACGAGGT CCTCTTCTCC 
CGGCGGGGCT CCAGCTACTG TGGCAAGAAT TGTGGAAAGC TGCAGACGAT ATTTCTGAAA 
TATCCGATGG AGGGGATGCC CAGAGCCAGG ATGCCAGTGC AGATTCGGGT CAGGCTGTGG 
TTCGGGCTCT CCGTGGATGA AAAGGAGTTC AATCAGTTTG CAGAGGGCAA GCTCTCTGTC 
TTTGCTGAAA CCTATGAGAA CCAGACCAAG CTGGCCCTGG TCGGAAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ACGGGCAAGA TCAAGCTCCC CAAGGACAGC 
TTCCGCCCCT CGGCCGGCTG GACCTGGGCT GGAGACTGGT TCGTGTGTCC GGAGAAGACC 
CTGCTTCATG ACACTGATGC GGGCCATCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAA 
ACCCGGCTCC CCGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACGGA TGTGAACGGG 
GAGAAGGTGC TTCCCAAGGA TGACATCGAA TGCCCACTGG GCTGGAAGTG GGAAGATGAG 
GAGTGGTCCA CGGACCTCAA TCGGGCTGTC GATGAACAAG GCTGGGAGTA CAGCATCACC 
ATCCCTCCGG ACCGGAAGCC GAGGCACTGG GTCCCTGCTG AGAAGATGTA CTACACACAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG AGGGACCTCA GCCAGATGGA GGCGCTGAAG 
AGGCACAGGC AGGCAGAGGC GGAGGGTGAA GGCTGGGAGT ACGCCTCACT CTTTGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACAGAC GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAGCCCC TGGAGAAGAC GGGACCTGCA GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC 
GGTGTGGTGG ACGACAGGAG TGAGGATTCC ATGTCTGTCT CCACCTTGAG CTTTGGCGTG 
AACAGACCCA CGATCTCCTG CATCTTCGAC TATGGAAACC GCTACCATCT ACGTTGCTAT 
ATGTACCAGG CCCGGGATCT GCCTGCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAATAC CCTGAACCCC 
ACCTGGGACC AGACACTCAT CTTCTACGAG ATTGAGATCT TCGGCGAGCC AGCCAGCATC 
GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG CTGTATGACC ATGACACCTA CGGCGCAGAT 
GAGTTCATGG GACGCTGCAT CTGTCAGCCG AGTCTGGAAC GGATGCCCCG GTTGGCCTGG 
TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG GGTGAGCTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAGA AGCCGGCTAT CCACCATATT CCCGGTTTTG AGGTGCAGGA TACATCAGGG 
ATCCTGGAAG AGTCGGAGGA CACGGACCTG CCCTACCCGC CACCCCAGAG GGAGGCCAAC 
ATCTACATGG TCCCCCAGAA CATCAAGCCG GTTCTCCAGC GAACAGCCAT TGAGATCCTG 
GCATGGGGGC TTCGGAACAT GAAGAGTTAC CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG 
GTGGTGGAGT GCGGGGGCCA GACGGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGCAC CCTCTTCATG GAAGTGATGC TGCCCCGGGA GGATCTCTAC 
TGTCCCCCCA TCGTGGTCAA GGTCATTGAT AACCGGCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGCTC CCTGGAGAGC TTCCTGTGTG ACCCTTACTC AGAGGAGAGT 
CCGTCCCCAC AGGGCGGCCC AGATGACGTG AGCTTGCTCA GTCCTGGGGA AGACGTGCTG 
ATTGACATCG ACGACAAGGA ACCCCTCATC CCCATCCAGC TTGCGGATGG TCTGTCGGGC 
TTGGCCCCCA CTAACATGGC GTCTTCTCCA TCCAGTCCTC ATGAGGAGGA GTTTATTGAC 
TGGTGGAGCA AATTCTTCGC CTCGATTGGG GAGAGGGAAA AGTGTGGGTC CTACCTGGAG 
AAGGACTTTG ACACTCTAAA GGTCTATGAT ACCCCGCTGG AGAATGTGAA GGCTTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGATG 
GAAGACCCAT CTGTCATTGG GGAATTTAAG GGCCTCTTCA AAATTTATCC CCTCCCAGAA 
GACCCTGCCA TTCCCATGCC TCCAAGACAG TTCCACCAGC TGGCCTCCCA GGAGCCCCAG 
GAGTGCCTGG TCCGCATCTA CATCATCCGA GCATTTGGCC TGCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCGGTGAG CGACCAAGAC 
AACTACATCC CCTGCACCCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACC CTCTATGACT ACGACCTCCT CTCCAAGGAT 
GAAAAGATCG GGGAGACGGT CATCGACCTG GAGAACAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGCC TCCCCCAGAC CTATTGCGTC TCTGGACCCA ACCAGTGGAG GGATCAGCTC 
CGTCCCTCCC AGCTTCTCCA CCTGTTCTGC CAGCAGCACA GGGTCAAGGC CCCCGTGTAC 
CGGACGGACC GCGTGGTGTT TCAGGATAAA GAATACACCA TTGAGGAAAT AGAGGCTGGC 
AGGGTCCCAA ACCCACACCT GGGCCCAGCA GAGGAGCGTC TGGCCTTGTA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA ACACGTGGAG TCCCGCCCCC TCTATAGCCC CCTGCAGCCG 
GACATCGAGC AGGGGAAGCT GCAGATGTGG ATCGACCTGT TTCCAAAGGC CCTGGGGCGG 
CCGGGACCTC CCTTCAACAT CACCCCACGG AGAGCCAGGA GGTTTTTCCT GCGTTGTATT 
ATCTGGAACA CCAAGGATGT GATTCTGGAT GACCTCAGCA TCACCGGGGA GAAGATGAGT 
GACATTTATG TGAAAGGCTG GATGGTGGGC TTTGAAGAAC ACAAGCAGAA GACAGATGTG 
CATTATCGGT CCCTGGGAGG CGAAGGCAAC TTTAACTGGA GGTTCGTTTT CCCTTTCGAC 
TACCTGCCAG CTGAGCAAGT CTGCACCGTC TCCAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAGATCCC TGCGCGAGTA GTCTTCCAGA TTTGGGACAA CGACAAGTTC 
TCCTTTGATG ACTTTTTGGG TGAGCACCAT TTCTGGTCTG TACCACTCTC CACTCGGGGC 
TGCCCCAGAA CCCACGCTTT GCGGTGTTGG ATGGTAAGAC TCAGGGTGGT GGTGGGGGGC 
ACAGGGTGGA GGCAGTGCAG GCTCCAGTGG GCCGTCACTG GCTGGCTGGC CTCTCACTTG 
ACAGGATCTC TACCACCTCA GTCGAGACAG CTTGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996046.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851500.1 Sus scrofa dysferlin (LOC100522732), transcript variant X9, mRNA.

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CloneID	OSe34208
Clone ID Related Accession (Same CDS sequence)	XM_013996046.1
Accession Version	XM_013996046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG GAGGAGGAGT TTATTGACTG GTGGAGCAAA 
TTCTTCGCCT CGATTGGGGA GAGGGAAAAG TGTGGGTCCT ACCTGGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGATAC CCCGCTGGAG AATGTGAAGG CTTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT 
GTCATTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCTGCCATT 
CCCATGCCTC CAAGACAGTT CCACCAGCTG GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGCG ACCAAGACAA CTACATCCCC 
TGCACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACCCT CTATGACTAC GACCTCCTCT CCAAGGATGA AAAGATCGGG 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC 
CCCCAGACCT ATTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG 
CTTCTCCACC TGTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCCAAAC 
CCACACCTGG GCCCAGCAGA GGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC TATAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGGAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TTCTGGATGA CCTCAGCATC ACCGGGGAGA AGATGAGTGA CATTTATGTG 
AAAGGCTGGA TGGTGGGCTT TGAAGAACAC AAGCAGAAGA CAGATGTGCA TTATCGGTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCGTTTTCC CTTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAGATCCCTG CGCGAGTAGT CTTCCAGATT TGGGACAACG ACAAGTTCTC CTTTGATGAC 
TTTTTGGGTG AGCACCATTT CTGGTCTGTA CCACTCTCCA CTCGGGGCTG CCCCAGAACC 
CACGCTTTGC GGTGTTGGAT GGTAAGACTC AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG 
CAGTGCAGGC TCCAGTGGGC CGTCACTGGC TGGCTGGCCT CTCACTTGAC AGGATCTCTA 
CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851500.1
CDS	143..6172
Translation	MLSCQLVRASNLPSVKKDRRSDPVASLTFRGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996046.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996046.1

ORF Insert Sequence:

ATGCTGAGTT GCCAGCTGGT GAGGGCCAGC AACCTCCCCA GTGTGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCTT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAACGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CGTCCCCCTG 
GACCAGAGCT CTGAGCTTCA TGTGGTGGTC AAAGACCACG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAACGT CCCACTCCGG GAGGTTCTCG CCACCCCCAG CCTGTCCGCC 
AGCTTCAATG CCCCACTGCT GGACACTAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACGCCACT TCCTGGAGCT GTGCCTGTGT TCCCACCCGC TACACCTCTG 
GAACCCTCCC CGACTCTGCC CGACATGGAT ATGGTGGCTG GTGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACGGAGG ACCAGGGGCT GACGGGAGAC 
GAGGCCGAGC CATTCCCGGA CCAGAGCGGA ACTCCGGGTC CAGGGGCCCC CATCACCCCA 
AAGAAGCCAC CTTCCCATCC TCCCCACCAC CGACCAGGGG GGAGAAGGAG GAGAAGCACG 
CCCGCGCCCA GAAAGCCGCT GTCGGACAAA CCTCAGGACT TCCAGATCAG GGTCCAGGTG 
ATCGAGGGAC GCCAGCTGCC GGGGGTGAAC ATCAAGCCTG TGGTCAAGGT CACCGCTGCC 
GGGCAGACCA AGCGGACTCG GATCCACAAG GGAAACAGCC CCCTCTTCAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTGCGGAG CTGTTTAATG AGCCCATCTT CATCACGGTG 
GTGGACTCCC GTTCCCTCAG GACAGACGCT CTCATCGGGG AGTTCCGGAT GGACGTGGGC 
AGCATCTACC GGGAACCCCG ACACGCCTAT CTCAGGAAGT GGCTGCTGCT CTCGGACCCA 
GACGACTTCT CTGCTGGGGC CAGAGGCTAC CTGAAAGCCA GCCTTTGCGT GCTGGGCCCT 
GGAGACGAGG CCCCTCTGGA GAGGAAAGAC CCCTCTGAGG ACAAGGAGGA CATCGAAGGC 
AACCTGCTCA GGCCGACGGG CGTGGCCCTC CGTGGAGCGA ACTTCTGCCT GAAGGTCTTC 
AGGGCGGAGG ACTTACCGCA GATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGGTTTGAGA GTAACAAGAA GAACTTGGTG GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTAT GTGGCAAGAT CCTGGAGAAG ACGGCCAACC CTCAGTGGAA CCAGAGTATC 
ACGCTGCCTG CCATGTTTCC CTCCATGTGT GAGAAGATGA GGATTCGTGT CATGGACTGG 
GACCGCCTCA CCCACAACGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAGTAGA AGTGGACGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTACGTCA ACCTGTACGG CAGCCCCAGG GAGTTCACTG GCTTCCCGGA CCCCTACGCG 
GAGCTCAACA CAGGCAAGGG GGAAGGCGTG GCCTACCGAG GCCGGCTGCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGAGCAG AAGGTGGAGG ATCTTCCTGC AGATGACATC 
CTTCGGGTGG AGAAGTACCT CCGGAGGCGC AAGTACTCGC TCTTTGCTGC CTTCTACTCG 
GCCACCATGC TACAGGACGT GGACGACGCC ATCCAGTTCG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACACGAC CTGCCTGCCT CTGGCCTCCA CCACCCAGTA CAGCCGGGCG 
GTCTTTGACG GATGCCACTA CTACTACTTA CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAC CGAGTGGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCCGACC GGCTGGAAGC CGGCCTGGAG CAGGTTCACT TGGCCCTGAG GGCGCAGTGC 
TCCACCGAGG ACGTGGACTC CCTGGCGGCT CAGCTGATGG ATGAGCTCAT TGCAGACTGC 
AGCCAGCCCC TAGGTGACAT CCATGAGACG CCCTCTGCTA CCCACCTGGA CCAGTACCTG 
CACCGGCTGC GTATGCGTCA CCTGAGCCAG ATCGTTGAGG CGGCCCAGGC CCTGAAGCTT 
GGCCACAGTG AACTCCCCGC TGCCCTGGAG CAGGCTGAGG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCAG ATGAGCCCCA GAACAGCCTG CCTGACATCG TCATCTGGAT GCTGCAGGGC 
GACAAGCGGG TGGCCTACCA GCGGGTGCCT GCCCACGAGG TCCTCTTCTC CCGGCGGGGC 
TCCAGCTACT GTGGCAAGAA TTGTGGAAAG CTGCAGACGA TATTTCTGAA ATATCCGATG 
GAGGGGATGC CCAGAGCCAG GATGCCAGTG CAGATTCGGG TCAGGCTGTG GTTCGGGCTC 
TCCGTGGATG AAAAGGAGTT CAATCAGTTT GCAGAGGGCA AGCTCTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GCTGGCCCTG GTCGGAAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGACTGG TTCGTGTGTC CGGAGAAGAC CCTGCTTCAT 
GACACTGATG CGGGCCATCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA AACCCGGCTC 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACGG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATCGA ATGCCCACTG GGCTGGAAGT GGGAAGATGA GGAGTGGTCC 
ACGGACCTCA ATCGGGCTGT CGATGAACAA GGCTGGGAGT ACAGCATCAC CATCCCTCCG 
GACCGGAAGC CGAGGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG GAGGGACCTC AGCCAGATGG AGGCGCTGAA GAGGCACAGG 
CAGGCAGAGG CGGAGGGTGA AGGCTGGGAG TACGCCTCAC TCTTTGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACAGA CGCCTTTCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGACCTGC AGCTGTGTTC GCCCTCGAGG GGGCCCTGGG CGGTGTGGTG 
GACGACAGGA GTGAGGATTC CATGTCTGTC TCCACCTTGA GCTTTGGCGT GAACAGACCC 
ACGATCTCCT GCATCTTCGA CTATGGAAAC CGCTACCATC TACGTTGCTA TATGTACCAG 
GCCCGGGATC TGCCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAATA CCCTGAACCC CACCTGGGAC 
CAGACACTCA TCTTCTACGA GATTGAGATC TTCGGCGAGC CAGCCAGCAT CGCCGAGCAG 
CCGCCCAGCA TCGTGGTGGA GCTGTATGAC CATGACACCT ACGGCGCAGA TGAGTTCATG 
GGACGCTGCA TCTGTCAGCC GAGTCTGGAA CGGATGCCCC GGTTGGCCTG GTTCCCTCTG 
ACTAGGGGCA GCCAGCCGGC GGGTGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCTA TCCACCATAT TCCCGGTTTT GAGGTGCAGG ATACATCAGG GATCCTGGAA 
GAGTCGGAGG ACACGGACCT GCCCTACCCG CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTCCCCCAGA ACATCAAGCC GGTTCTCCAG CGAACAGCCA TTGAGATCCT GGCATGGGGG 
CTTCGGAACA TGAAGAGTTA CCAGCTGGCC AGTGTGTCTT CCCCCAGCCT GGTGGTGGAG 
TGCGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCCGGG AGGATCTCTA CTGTCCCCCC 
ATCGTGGTCA AGGTCATTGA TAACCGGCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCTTACT CAGAGGAGAG TCCGTCCCCA 
CAGGGCGGCC CAGATGACGT GAGCTTGCTC AGTCCTGGGG AAGACGTGCT GATTGACATC 
GACGACAAGG AACCCCTCAT CCCCATCCAG GAGGAGGAGT TTATTGACTG GTGGAGCAAA 
TTCTTCGCCT CGATTGGGGA GAGGGAAAAG TGTGGGTCCT ACCTGGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGATAC CCCGCTGGAG AATGTGAAGG CTTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT 
GTCATTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCTGCCATT 
CCCATGCCTC CAAGACAGTT CCACCAGCTG GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGCG ACCAAGACAA CTACATCCCC 
TGCACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACCCT CTATGACTAC GACCTCCTCT CCAAGGATGA AAAGATCGGG 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC 
CCCCAGACCT ATTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG 
CTTCTCCACC TGTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCCAAAC 
CCACACCTGG GCCCAGCAGA GGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC TATAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGGAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TTCTGGATGA CCTCAGCATC ACCGGGGAGA AGATGAGTGA CATTTATGTG 
AAAGGCTGGA TGGTGGGCTT TGAAGAACAC AAGCAGAAGA CAGATGTGCA TTATCGGTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCGTTTTCC CTTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAGATCCCTG CGCGAGTAGT CTTCCAGATT TGGGACAACG ACAAGTTCTC CTTTGATGAC 
TTTTTGGGTG AGCACCATTT CTGGTCTGTA CCACTCTCCA CTCGGGGCTG CCCCAGAACC 
CACGCTTTGC GGTGTTGGAT GGTAAGACTC AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG 
CAGTGCAGGC TCCAGTGGGC CGTCACTGGC TGGCTGGCCT CTCACTTGAC AGGATCTCTA 
CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996047.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851501.1 Sus scrofa dysferlin (LOC100522732), transcript variant X10, mRNA.

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CloneID	OSe34209
Clone ID Related Accession (Same CDS sequence)	XM_013996047.1
Accession Version	XM_013996047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGT GGACGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TACGTCAACC TGTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCGGAG 
CTCAACACAG GCAAGGGGGA AGGCGTGGCC TACCGAGGCC GGCTGCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGATC TTCCTGCAGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCTGCCTT CTACTCGGCC 
ACCATGCTAC AGGACGTGGA CGACGCCATC CAGTTCGAGG TCAGCATCGG GAACTACGGG 
AACAAGTTCG ACACGACCTG CCTGCCTCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACCGA GTGGAGGCTC AGAACCAGCT GCTCAGGATC 
GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTTCACTTGG CCCTGAGGGC GCAGTGCTCC 
ACCGAGGACG TGGACTCCCT GGCGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC 
CAGCCCCTAG GTGACATCCA TGAGACGCCC TCTGCTACCC ACCTGGACCA GTACCTGCAC 
CGGCTGCGTA TGCGTCACCT GAGCCAGATC GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC 
CACAGTGAAC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT GCAGGGCGAC 
AAGCGGGTGG CCTACCAGCG GGTGCCTGCC CACGAGGTCC TCTTCTCCCG GCGGGGCTCC 
AGCTACTGTG GCAAGAATTG TGGAAAGCTG CAGACGATAT TTCTGAAATA TCCGATGGAG 
GGGATGCCCA GAGCCAGGAT GCCAGTGCAG ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC 
GTGGATGAAA AGGAGTTCAA TCAGTTTGCA GAGGGCAAGC TCTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGCT GGCCCTGGTC GGAAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGCAAGATC AAGCTCCCCA AGGACAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCGG AGAAGACCCT GCTTCATGAC 
ACTGATGCGG GCCATCTGAG CTTCGTGGAG GAGGTGTTTG AGAACCAAAC CCGGCTCCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAATG CCCACTGGGC TGGAAGTGGG AAGATGAGGA GTGGTCCACG 
GACCTCAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCTGAG AAGATGTACT ACACACACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC GCCTCACTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGACGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GACCTGCAGC TGTGTTCGCC CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC 
GACAGGAGTG AGGATTCCAT GTCTGTCTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATCTCCTGCA TCTTCGACTA TGGAAACCGC TACCATCTAC GTTGCTATAT GTACCAGGCC 
CGGGATCTGC CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC AGTGGTGGCG AAGAATACCC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTACGAGAT TGAGATCTTC GGCGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTACG GCGCAGATGA GTTCATGGGA 
CGCTGCATCT GTCAGCCGAG TCTGGAACGG ATGCCCCGGT TGGCCTGGTT CCCTCTGACT 
AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCTATCC ACCATATTCC CGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC CTACCCGCCA CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACAGCCATTG AGATCCTGGC ATGGGGGCTT 
CGGAACATGA AGAGTTACCA GCTGGCCAGT GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCCGGGAGG ATCTCTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGGCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGACGTGAG CTTGCTCAGT CCTGGGGAAG ACGTGCTGAT TGACATCGAC 
GACAAGGAAC CCCTCATCCC CATCCAGCTT GCGGATGGTC TGTCGGGCTT GGCCCCCACT 
AACATGGCGT CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TTATTGACTG GTGGAGCAAA 
TTCTTCGCCT CGATTGGGGA GAGGGAAAAG TGTGGGTCCT ACCTGGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGATAC CCCGCTGGAG AATGTGAAGG CTTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT 
GTCATTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCTGCCATT 
CCCATGCCTC CAAGACAGTT CCACCAGCTG GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGCG ACCAAGACAA CTACATCCCC 
TGCACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACCCT CTATGACTAC GACCTCCTCT CCAAGGATGA AAAGATCGGG 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC 
CCCCAGACCT ATTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG 
CTTCTCCACC TGTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCCAAAC 
CCACACCTGG GCCCAGCAGA GGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC TATAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGGAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TTCTGGATGA CCTCAGCATC ACCGGGGAGA AGATGAGTGA CATTTATGTG 
AAAGGCTGGA TGGTGGGCTT TGAAGAACAC AAGCAGAAGA CAGATGTGCA TTATCGGTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCGTTTTCC CTTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAGATCCCTG CGCGAGTAGT CTTCCAGATT TGGGACAACG ACAAGTTCTC CTTTGATGAC 
TTTTTGGGTG AGCACCATTT CTGGTCTGTA CCACTCTCCA CTCGGGGCTG CCCCAGAACC 
CACGCTTTGC GGTGTTGGAT GGTAAGACTC AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG 
CAGTGCAGGC TCCAGTGGGC CGTCACTGGC TGGCTGGCCT CTCACTTGAC AGGATCTCTA 
CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851501.1
CDS	527..6616
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996047.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996047.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGT GGACGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TACGTCAACC TGTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCGGAG 
CTCAACACAG GCAAGGGGGA AGGCGTGGCC TACCGAGGCC GGCTGCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGATC TTCCTGCAGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCTGCCTT CTACTCGGCC 
ACCATGCTAC AGGACGTGGA CGACGCCATC CAGTTCGAGG TCAGCATCGG GAACTACGGG 
AACAAGTTCG ACACGACCTG CCTGCCTCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACCGA GTGGAGGCTC AGAACCAGCT GCTCAGGATC 
GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTTCACTTGG CCCTGAGGGC GCAGTGCTCC 
ACCGAGGACG TGGACTCCCT GGCGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC 
CAGCCCCTAG GTGACATCCA TGAGACGCCC TCTGCTACCC ACCTGGACCA GTACCTGCAC 
CGGCTGCGTA TGCGTCACCT GAGCCAGATC GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC 
CACAGTGAAC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT GCAGGGCGAC 
AAGCGGGTGG CCTACCAGCG GGTGCCTGCC CACGAGGTCC TCTTCTCCCG GCGGGGCTCC 
AGCTACTGTG GCAAGAATTG TGGAAAGCTG CAGACGATAT TTCTGAAATA TCCGATGGAG 
GGGATGCCCA GAGCCAGGAT GCCAGTGCAG ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC 
GTGGATGAAA AGGAGTTCAA TCAGTTTGCA GAGGGCAAGC TCTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGCT GGCCCTGGTC GGAAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGCAAGATC AAGCTCCCCA AGGACAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCGG AGAAGACCCT GCTTCATGAC 
ACTGATGCGG GCCATCTGAG CTTCGTGGAG GAGGTGTTTG AGAACCAAAC CCGGCTCCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAATG CCCACTGGGC TGGAAGTGGG AAGATGAGGA GTGGTCCACG 
GACCTCAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCTGAG AAGATGTACT ACACACACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC GCCTCACTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGACGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GACCTGCAGC TGTGTTCGCC CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC 
GACAGGAGTG AGGATTCCAT GTCTGTCTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATCTCCTGCA TCTTCGACTA TGGAAACCGC TACCATCTAC GTTGCTATAT GTACCAGGCC 
CGGGATCTGC CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC AGTGGTGGCG AAGAATACCC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTACGAGAT TGAGATCTTC GGCGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTACG GCGCAGATGA GTTCATGGGA 
CGCTGCATCT GTCAGCCGAG TCTGGAACGG ATGCCCCGGT TGGCCTGGTT CCCTCTGACT 
AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCTATCC ACCATATTCC CGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC CTACCCGCCA CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACAGCCATTG AGATCCTGGC ATGGGGGCTT 
CGGAACATGA AGAGTTACCA GCTGGCCAGT GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCCGGGAGG ATCTCTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGGCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGACGTGAG CTTGCTCAGT CCTGGGGAAG ACGTGCTGAT TGACATCGAC 
GACAAGGAAC CCCTCATCCC CATCCAGCTT GCGGATGGTC TGTCGGGCTT GGCCCCCACT 
AACATGGCGT CTTCTCCATC CAGTCCTCAT GAGGAGGAGT TTATTGACTG GTGGAGCAAA 
TTCTTCGCCT CGATTGGGGA GAGGGAAAAG TGTGGGTCCT ACCTGGAGAA GGACTTTGAC 
ACTCTAAAGG TCTATGATAC CCCGCTGGAG AATGTGAAGG CTTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGATGGA AGACCCATCT 
GTCATTGGGG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCTGCCATT 
CCCATGCCTC CAAGACAGTT CCACCAGCTG GCCTCCCAGG AGCCCCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCGGTGAGCG ACCAAGACAA CTACATCCCC 
TGCACCCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACCCT CTATGACTAC GACCTCCTCT CCAAGGATGA AAAGATCGGG 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGCCTC 
CCCCAGACCT ATTGCGTCTC TGGACCCAAC CAGTGGAGGG ATCAGCTCCG TCCCTCCCAG 
CTTCTCCACC TGTTCTGCCA GCAGCACAGG GTCAAGGCCC CCGTGTACCG GACGGACCGC 
GTGGTGTTTC AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAG GGTCCCAAAC 
CCACACCTGG GCCCAGCAGA GGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAAC ACGTGGAGTC CCGCCCCCTC TATAGCCCCC TGCAGCCGGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGAT CGACCTGTTT CCAAAGGCCC TGGGGCGGCC GGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGGAGG TTTTTCCTGC GTTGTATTAT CTGGAACACC 
AAGGATGTGA TTCTGGATGA CCTCAGCATC ACCGGGGAGA AGATGAGTGA CATTTATGTG 
AAAGGCTGGA TGGTGGGCTT TGAAGAACAC AAGCAGAAGA CAGATGTGCA TTATCGGTCC 
CTGGGAGGCG AAGGCAACTT TAACTGGAGG TTCGTTTTCC CTTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GCACCGTCTC CAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAGATCCCTG CGCGAGTAGT CTTCCAGATT TGGGACAACG ACAAGTTCTC CTTTGATGAC 
TTTTTGGGTG AGCACCATTT CTGGTCTGTA CCACTCTCCA CTCGGGGCTG CCCCAGAACC 
CACGCTTTGC GGTGTTGGAT GGTAAGACTC AGGGTGGTGG TGGGGGGCAC AGGGTGGAGG 
CAGTGCAGGC TCCAGTGGGC CGTCACTGGC TGGCTGGCCT CTCACTTGAC AGGATCTCTA 
CCACCTCAGT CGAGACAGCT TGGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996049.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851503.1 Sus scrofa dysferlin (LOC100522732), transcript variant X12, mRNA.

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CloneID	OSe34211
Clone ID Related Accession (Same CDS sequence)	XM_013996049.1
Accession Version	XM_013996049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA GGTGCGGTCA AGCCTTCTTC AGCCACAGAC 
CTGGACGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTACGTCAA CCTGTACGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCGG AGCTCAACAC AGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG CCGGCTGCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCTGCC TTCTACTCGG CCACCATGCT ACAGGACGTG 
GACGACGCCA TCCAGTTCGA GGTCAGCATC GGGAACTACG GGAACAAGTT CGACACGACC 
TGCCTGCCTC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACC GAGTGGAGGC TCAGAACCAG CTGCTCAGGA TCGCCGACCG GCTGGAAGCC 
GGCCTGGAGC AGGTTCACTT GGCCCTGAGG GCGCAGTGCT CCACCGAGGA CGTGGACTCC 
CTGGCGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT AGGTGACATC 
CATGAGACGC CCTCTGCTAC CCACCTGGAC CAGTACCTGC ACCGGCTGCG TATGCGTCAC 
CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC CTGAAGCTTG GCCACAGTGA ACTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTGCAGGGCG ACAAGCGGGT GGCCTACCAG 
CGGGTGCCTG CCCACGAGGT CCTCTTCTCC CGGCGGGGCT CCAGCTACTG TGGCAAGAAT 
TGTGGAAAGC TGCAGACGAT ATTTCTGAAA TATCCGATGG AGGGGATGCC CAGAGCCAGG 
ATGCCAGTGC AGATTCGGGT CAGGCTGTGG TTCGGGCTCT CCGTGGATGA AAAGGAGTTC 
AATCAGTTTG CAGAGGGCAA GCTCTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAG 
CTGGCCCTGG TCGGAAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGCAAGA TCAAGCTCCC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC GGAGAAGACC CTGCTTCATG ACACTGATGC GGGCCATCTG 
AGCTTCGTGG AGGAGGTGTT TGAGAACCAA ACCCGGCTCC CCGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAA 
TGCCCACTGG GCTGGAAGTG GGAAGATGAG GAGTGGTCCA CGGACCTCAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCTG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGTGAA 
GGCTGGGAGT ACGCCTCACT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAC 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCCC TGGAGAAGAC GGGACCTGCA 
GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC GGTGTGGTGG ACGACAGGAG TGAGGATTCC 
ATGTCTGTCT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATCTCCTG CATCTTCGAC 
TATGGAAACC GCTACCATCT ACGTTGCTAT ATGTACCAGG CCCGGGATCT GCCTGCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAATAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTACGAG 
ATTGAGATCT TCGGCGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGCGCAGAT GAGTTCATGG GACGCTGCAT CTGTCAGCCG 
AGTCTGGAAC GGATGCCCCG GTTGGCCTGG TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCTAT CCACCATATT 
CCCGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCCTACCCGC CACCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACAGCCAT TGAGATCCTG GCATGGGGGC TTCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCCGGGA GGATCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGGCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGAGC 
TTCCTGTGTG ACCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGACGTG 
AGCTTGCTCA GTCCTGGGGA AGACGTGCTG ATTGACATCG ACGACAAGGA ACCCCTCATC 
CCCATCCAGG AGGAGGAGTT TATTGACTGG TGGAGCAAAT TCTTCGCCTC GATTGGGGAG 
AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG GACTTTGACA CTCTAAAGGT CTATGATACC 
CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGATGGAA GACCCATCTG TCATTGGGGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCTGCCATTC CCATGCCTCC AAGACAGTTC 
CACCAGCTGG CCTCCCAGGA GCCCCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGCGA CCAAGACAAC TACATCCCCT GCACCCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACCCTC 
TATGACTACG ACCTCCTCTC CAAGGATGAA AAGATCGGGG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGCCTCC CCCAGACCTA TTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGT CCCTCCCAGC TTCTCCACCT GTTCTGCCAG 
CAGCACAGGG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAGG GTCCCAAACC CACACCTGGG CCCAGCAGAG 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAACA CGTGGAGTCC 
CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGATC 
GACCTGTTTC CAAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGGAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT TCTGGATGAC 
CTCAGCATCA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GGTGGGCTTT 
GAAGAACACA AGCAGAAGAC AGATGTGCAT TATCGGTCCC TGGGAGGCGA AGGCAACTTT 
AACTGGAGGT TCGTTTTCCC TTTCGACTAC CTGCCAGCTG AGCAAGTCTG CACCGTCTCC 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AGATCCCTGC GCGAGTAGTC 
TTCCAGATTT GGGACAACGA CAAGTTCTCC TTTGATGACT TTTTGGGTGA GCACCATTTC 
TGGTCTGTAC CACTCTCCAC TCGGGGCTGC CCCAGAACCC ACGCTTTGCG GTGTTGGATG 
GTAAGACTCA GGGTGGTGGT GGGGGGCACA GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC 
GTCACTGGCT GGCTGGCCTC TCACTTGACA GGATCTCTAC CACCTCAGTC GAGACAGCTT 
GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851503.1
CDS	527..6595
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996049.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996049.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGA GGAGCCTGCA GGTGCGGTCA AGCCTTCTTC AGCCACAGAC 
CTGGACGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTACGTCAA CCTGTACGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCGG AGCTCAACAC AGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG CCGGCTGCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA TCTTCCTGCA GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTACTCGCT CTTTGCTGCC TTCTACTCGG CCACCATGCT ACAGGACGTG 
GACGACGCCA TCCAGTTCGA GGTCAGCATC GGGAACTACG GGAACAAGTT CGACACGACC 
TGCCTGCCTC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG ATGCCACTAC 
TACTACTTAC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCCTA CTGGGAGGAC 
ATCAGCCACC GAGTGGAGGC TCAGAACCAG CTGCTCAGGA TCGCCGACCG GCTGGAAGCC 
GGCCTGGAGC AGGTTCACTT GGCCCTGAGG GCGCAGTGCT CCACCGAGGA CGTGGACTCC 
CTGGCGGCTC AGCTGATGGA TGAGCTCATT GCAGACTGCA GCCAGCCCCT AGGTGACATC 
CATGAGACGC CCTCTGCTAC CCACCTGGAC CAGTACCTGC ACCGGCTGCG TATGCGTCAC 
CTGAGCCAGA TCGTTGAGGC GGCCCAGGCC CTGAAGCTTG GCCACAGTGA ACTCCCCGCT 
GCCCTGGAGC AGGCTGAGGA CTGGCTCCTG CGTCTCCGTG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CTGACATCGT CATCTGGATG CTGCAGGGCG ACAAGCGGGT GGCCTACCAG 
CGGGTGCCTG CCCACGAGGT CCTCTTCTCC CGGCGGGGCT CCAGCTACTG TGGCAAGAAT 
TGTGGAAAGC TGCAGACGAT ATTTCTGAAA TATCCGATGG AGGGGATGCC CAGAGCCAGG 
ATGCCAGTGC AGATTCGGGT CAGGCTGTGG TTCGGGCTCT CCGTGGATGA AAAGGAGTTC 
AATCAGTTTG CAGAGGGCAA GCTCTCTGTC TTTGCTGAAA CCTATGAGAA CCAGACCAAG 
CTGGCCCTGG TCGGAAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGCAAGA TCAAGCTCCC CAAGGACAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC GGAGAAGACC CTGCTTCATG ACACTGATGC GGGCCATCTG 
AGCTTCGTGG AGGAGGTGTT TGAGAACCAA ACCCGGCTCC CCGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAACGGG GAGAAGGTGC TTCCCAAGGA TGACATCGAA 
TGCCCACTGG GCTGGAAGTG GGAAGATGAG GAGTGGTCCA CGGACCTCAA TCGGGCTGTC 
GATGAACAAG GCTGGGAGTA CAGCATCACC ATCCCTCCGG ACCGGAAGCC GAGGCACTGG 
GTCCCTGCTG AGAAGATGTA CTACACACAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCGCTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGTGAA 
GGCTGGGAGT ACGCCTCACT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACAGAC 
GCCTTTCGCC GCCGCCGCTG GCGCCGCCGC ATGGAGCCCC TGGAGAAGAC GGGACCTGCA 
GCTGTGTTCG CCCTCGAGGG GGCCCTGGGC GGTGTGGTGG ACGACAGGAG TGAGGATTCC 
ATGTCTGTCT CCACCTTGAG CTTTGGCGTG AACAGACCCA CGATCTCCTG CATCTTCGAC 
TATGGAAACC GCTACCATCT ACGTTGCTAT ATGTACCAGG CCCGGGATCT GCCTGCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAATAC CCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTACGAG 
ATTGAGATCT TCGGCGAGCC AGCCAGCATC GCCGAGCAGC CGCCCAGCAT CGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGCGCAGAT GAGTTCATGG GACGCTGCAT CTGTCAGCCG 
AGTCTGGAAC GGATGCCCCG GTTGGCCTGG TTCCCTCTGA CTAGGGGCAG CCAGCCGGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCTAT CCACCATATT 
CCCGGTTTTG AGGTGCAGGA TACATCAGGG ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCCTACCCGC CACCCCAGAG GGAGGCCAAC ATCTACATGG TCCCCCAGAA CATCAAGCCG 
GTTCTCCAGC GAACAGCCAT TGAGATCCTG GCATGGGGGC TTCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTGTGTCTTC CCCCAGCCTG GTGGTGGAGT GCGGGGGCCA GACGGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTCG ACATCTGCAC CCTCTTCATG 
GAAGTGATGC TGCCCCGGGA GGATCTCTAC TGTCCCCCCA TCGTGGTCAA GGTCATTGAT 
AACCGGCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCATCCGCTC CCTGGAGAGC 
TTCCTGTGTG ACCCTTACTC AGAGGAGAGT CCGTCCCCAC AGGGCGGCCC AGATGACGTG 
AGCTTGCTCA GTCCTGGGGA AGACGTGCTG ATTGACATCG ACGACAAGGA ACCCCTCATC 
CCCATCCAGG AGGAGGAGTT TATTGACTGG TGGAGCAAAT TCTTCGCCTC GATTGGGGAG 
AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG GACTTTGACA CTCTAAAGGT CTATGATACC 
CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGATGGAA GACCCATCTG TCATTGGGGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCTGCCATTC CCATGCCTCC AAGACAGTTC 
CACCAGCTGG CCTCCCAGGA GCCCCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CGGTGAGCGA CCAAGACAAC TACATCCCCT GCACCCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACCCTC 
TATGACTACG ACCTCCTCTC CAAGGATGAA AAGATCGGGG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGCCTCC CCCAGACCTA TTGCGTCTCT 
GGACCCAACC AGTGGAGGGA TCAGCTCCGT CCCTCCCAGC TTCTCCACCT GTTCTGCCAG 
CAGCACAGGG TCAAGGCCCC CGTGTACCGG ACGGACCGCG TGGTGTTTCA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAGG GTCCCAAACC CACACCTGGG CCCAGCAGAG 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAACA CGTGGAGTCC 
CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC ATCGAGCAGG GGAAGCTGCA GATGTGGATC 
GACCTGTTTC CAAAGGCCCT GGGGCGGCCG GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGGAGGT TTTTCCTGCG TTGTATTATC TGGAACACCA AGGATGTGAT TCTGGATGAC 
CTCAGCATCA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GGTGGGCTTT 
GAAGAACACA AGCAGAAGAC AGATGTGCAT TATCGGTCCC TGGGAGGCGA AGGCAACTTT 
AACTGGAGGT TCGTTTTCCC TTTCGACTAC CTGCCAGCTG AGCAAGTCTG CACCGTCTCC 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AGATCCCTGC GCGAGTAGTC 
TTCCAGATTT GGGACAACGA CAAGTTCTCC TTTGATGACT TTTTGGGTGA GCACCATTTC 
TGGTCTGTAC CACTCTCCAC TCGGGGCTGC CCCAGAACCC ACGCTTTGCG GTGTTGGATG 
GTAAGACTCA GGGTGGTGGT GGGGGGCACA GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC 
GTCACTGGCT GGCTGGCCTC TCACTTGACA GGATCTCTAC CACCTCAGTC GAGACAGCTT 
GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996051.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851505.1 Sus scrofa dysferlin (LOC100522732), transcript variant X14, mRNA.

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CloneID	OSe34213
Clone ID Related Accession (Same CDS sequence)	XM_013996051.1
Accession Version	XM_013996051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGCTTG CGGATGGTCT GTCGGGCTTG 
GCCCCCACTA ACATGGCGTC TTCTCCATCC AGTCCTCATA AGATCCTCCT GGAGGAGGAG 
TTTATTGACT GGTGGAGCAA ATTCTTCGCC TCGATTGGGG AGAGGGAAAA GTGTGGGTCC 
TACCTGGAGA AGGACTTTGA CACTCTAAAG GTCTATGATA CCCCGCTGGA GAATGTGAAG 
GCTTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGATGG AAGACCCATC TGTCATTGGG GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCTGCCAT TCCCATGCCT CCAAGACAGT TCCACCAGCT GGCCTCCCAG 
GAGCCCCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGC 
GACCAAGACA ACTACATCCC CTGCACCCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACCC TCTATGACTA CGACCTCCTC 
TCCAAGGATG AAAAGATCGG GGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGCGGCCT CCCCCAGACC TATTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GTCCCTCCCA GCTTCTCCAC CTGTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA GGGTCCCAAA CCCACACCTG GGCCCAGCAG AGGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAA CACGTGGAGT CCCGCCCCCT CTATAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGA TCGACCTGTT TCCAAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGGAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATTCTGGATG ACCTCAGCAT CACCGGGGAG 
AAGATGAGTG ACATTTATGT GAAAGGCTGG ATGGTGGGCT TTGAAGAACA CAAGCAGAAG 
ACAGATGTGC ATTATCGGTC CCTGGGAGGC GAAGGCAACT TTAACTGGAG GTTCGTTTTC 
CCTTTCGACT ACCTGCCAGC TGAGCAAGTC TGCACCGTCT CCAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAGATCCCT GCGCGAGTAG TCTTCCAGAT TTGGGACAAC 
GACAAGTTCT CCTTTGATGA CTTTTTGGGT GAGCACCATT TCTGGTCTGT ACCACTCTCC 
ACTCGGGGCT GCCCCAGAAC CCACGCTTTG CGGTGTTGGA TGGTAAGACT CAGGGTGGTG 
GTGGGGGGCA CAGGGTGGAG GCAGTGCAGG CTCCAGTGGG CCGTCACTGG CTGGCTGGCC 
TCTCACTTGA CAGGATCTCT ACCACCTCAG TCGAGACAGC TTGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851505.1
CDS	527..6577
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHKILLEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996051.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996051.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGCTTG CGGATGGTCT GTCGGGCTTG 
GCCCCCACTA ACATGGCGTC TTCTCCATCC AGTCCTCATA AGATCCTCCT GGAGGAGGAG 
TTTATTGACT GGTGGAGCAA ATTCTTCGCC TCGATTGGGG AGAGGGAAAA GTGTGGGTCC 
TACCTGGAGA AGGACTTTGA CACTCTAAAG GTCTATGATA CCCCGCTGGA GAATGTGAAG 
GCTTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGATGG AAGACCCATC TGTCATTGGG GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCTGCCAT TCCCATGCCT CCAAGACAGT TCCACCAGCT GGCCTCCCAG 
GAGCCCCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCGGTGAGC 
GACCAAGACA ACTACATCCC CTGCACCCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACCC TCTATGACTA CGACCTCCTC 
TCCAAGGATG AAAAGATCGG GGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGCGGCCT CCCCCAGACC TATTGCGTCT CTGGACCCAA CCAGTGGAGG 
GATCAGCTCC GTCCCTCCCA GCTTCTCCAC CTGTTCTGCC AGCAGCACAG GGTCAAGGCC 
CCCGTGTACC GGACGGACCG CGTGGTGTTT CAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA GGGTCCCAAA CCCACACCTG GGCCCAGCAG AGGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAA CACGTGGAGT CCCGCCCCCT CTATAGCCCC 
CTGCAGCCGG ACATCGAGCA GGGGAAGCTG CAGATGTGGA TCGACCTGTT TCCAAAGGCC 
CTGGGGCGGC CGGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGGAG GTTTTTCCTG 
CGTTGTATTA TCTGGAACAC CAAGGATGTG ATTCTGGATG ACCTCAGCAT CACCGGGGAG 
AAGATGAGTG ACATTTATGT GAAAGGCTGG ATGGTGGGCT TTGAAGAACA CAAGCAGAAG 
ACAGATGTGC ATTATCGGTC CCTGGGAGGC GAAGGCAACT TTAACTGGAG GTTCGTTTTC 
CCTTTCGACT ACCTGCCAGC TGAGCAAGTC TGCACCGTCT CCAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAGATCCCT GCGCGAGTAG TCTTCCAGAT TTGGGACAAC 
GACAAGTTCT CCTTTGATGA CTTTTTGGGT GAGCACCATT TCTGGTCTGT ACCACTCTCC 
ACTCGGGGCT GCCCCAGAAC CCACGCTTTG CGGTGTTGGA TGGTAAGACT CAGGGTGGTG 
GTGGGGGGCA CAGGGTGGAG GCAGTGCAGG CTCCAGTGGG CCGTCACTGG CTGGCTGGCC 
TCTCACTTGA CAGGATCTCT ACCACCTCAG TCGAGACAGC TTGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996052.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851506.1 Sus scrofa dysferlin (LOC100522732), transcript variant X15, mRNA.

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CloneID	OSe34214
Clone ID Related Accession (Same CDS sequence)	XM_013996052.1
Accession Version	XM_013996052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGCTTG CGGATGGTCT GTCGGGCTTG 
GCCCCCACTA ACATGGCGTC TTCTCCATCC AGTCCTCATG AGGAGGAGTT TATTGACTGG 
TGGAGCAAAT TCTTCGCCTC GATTGGGGAG AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGATACC CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA 
GACCCATCTG TCATTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC 
CCTGCCATTC CCATGCCTCC AAGACAGTTC CACCAGCTGG CCTCCCAGGA GCCCCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGCGA CCAAGACAAC 
TACATCCCCT GCACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACCCTC TATGACTACG ACCTCCTCTC CAAGGATGAA 
AAGATCGGGG AGACGGTCAT CGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGCCTCC CCCAGACCTA TTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGT 
CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCCAAACC CACACCTGGG CCCAGCAGAG GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAACA CGTGGAGTCC CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGGAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT TCTGGATGAC CTCAGCATCA CCGGGGAGAA GATGAGTGAC 
ATTTATGTGA AAGGCTGGAT GGTGGGCTTT GAAGAACACA AGCAGAAGAC AGATGTGCAT 
TATCGGTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCGTTTTCCC TTTCGACTAC 
CTGCCAGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AGATCCCTGC GCGAGTAGTC TTCCAGATTT GGGACAACGA CAAGTTCTCC 
TTTGATGACT TTTTGGGTGA GCACCATTTC TGGTCTGTAC CACTCTCCAC TCGGGGCTGC 
CCCAGAACCC ACGCTTTGCG GTGTTGGATG GTAAGACTCA GGGTGGTGGT GGGGGGCACA 
GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC GTCACTGGCT GGCTGGCCTC TCACTTGACA 
GGATCTCTAC CACCTCAGTC GAGACAGCTT GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851506.1
CDS	527..6565
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVADTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEEEPAGAVKPSSATDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGLAPTNMASSPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996052.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996052.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGACA CAGGAGGAGA GGAGGACACG 
GAGGACCAGG GGCTGACGGG AGACGAGGCC GAGCCATTCC CGGACCAGAG CGGAACTCCG 
GGTCCAGGGG CCCCCATCAC CCCAAAGAAG CCACCTTCCC ATCCTCCCCA CCACCGACCA 
GGGGGGAGAA GGAGGAGAAG CACGCCCGCG CCCAGAAAGC CGCTGTCGGA CAAACCTCAG 
GACTTCCAGA TCAGGGTCCA GGTGATCGAG GGACGCCAGC TGCCGGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACCGC TGCCGGGCAG ACCAAGCGGA CTCGGATCCA CAAGGGAAAC 
AGCCCCCTCT TCAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTGC GGAGCTGTTT 
AATGAGCCCA TCTTCATCAC GGTGGTGGAC TCCCGTTCCC TCAGGACAGA CGCTCTCATC 
GGGGAGTTCC GGATGGACGT GGGCAGCATC TACCGGGAAC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TGCTCTCGGA CCCAGACGAC TTCTCTGCTG GGGCCAGAGG CTACCTGAAA 
GCCAGCCTTT GCGTGCTGGG CCCTGGAGAC GAGGCCCCTC TGGAGAGGAA AGACCCCTCT 
GAGGACAAGG AGGACATCGA AGGCAACCTG CTCAGGCCGA CGGGCGTGGC CCTCCGTGGA 
GCGAACTTCT GCCTGAAGGT CTTCAGGGCG GAGGACTTAC CGCAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGGTTT GAGAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTATGTGGCA AGATCCTGGA GAAGACGGCC 
AACCCTCAGT GGAACCAGAG TATCACGCTG CCTGCCATGT TTCCCTCCAT GTGTGAGAAG 
ATGAGGATTC GTGTCATGGA CTGGGACCGC CTCACCCACA ACGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAG TAGAAGAGGA GCCTGCAGGT 
GCGGTCAAGC CTTCTTCAGC CACAGACCTG GACGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT ACGTCAACCT GTACGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCGGAGC TCAACACAGG CAAGGGGGAA GGCGTGGCCT ACCGAGGCCG GCTGCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGATCT TCCTGCAGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ACTCGCTCTT TGCTGCCTTC 
TACTCGGCCA CCATGCTACA GGACGTGGAC GACGCCATCC AGTTCGAGGT CAGCATCGGG 
AACTACGGGA ACAAGTTCGA CACGACCTGC CTGCCTCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGATG CCACTACTAC TACTTACCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCCTACTG GGAGGACATC AGCCACCGAG TGGAGGCTCA GAACCAGCTG 
CTCAGGATCG CCGACCGGCT GGAAGCCGGC CTGGAGCAGG TTCACTTGGC CCTGAGGGCG 
CAGTGCTCCA CCGAGGACGT GGACTCCCTG GCGGCTCAGC TGATGGATGA GCTCATTGCA 
GACTGCAGCC AGCCCCTAGG TGACATCCAT GAGACGCCCT CTGCTACCCA CCTGGACCAG 
TACCTGCACC GGCTGCGTAT GCGTCACCTG AGCCAGATCG TTGAGGCGGC CCAGGCCCTG 
AAGCTTGGCC ACAGTGAACT CCCCGCTGCC CTGGAGCAGG CTGAGGACTG GCTCCTGCGT 
CTCCGTGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCTG ACATCGTCAT CTGGATGCTG 
CAGGGCGACA AGCGGGTGGC CTACCAGCGG GTGCCTGCCC ACGAGGTCCT CTTCTCCCGG 
CGGGGCTCCA GCTACTGTGG CAAGAATTGT GGAAAGCTGC AGACGATATT TCTGAAATAT 
CCGATGGAGG GGATGCCCAG AGCCAGGATG CCAGTGCAGA TTCGGGTCAG GCTGTGGTTC 
GGGCTCTCCG TGGATGAAAA GGAGTTCAAT CAGTTTGCAG AGGGCAAGCT CTCTGTCTTT 
GCTGAAACCT ATGAGAACCA GACCAAGCTG GCCCTGGTCG GAAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGCAAGATCA AGCTCCCCAA GGACAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCGGA GAAGACCCTG 
CTTCATGACA CTGATGCGGG CCATCTGAGC TTCGTGGAGG AGGTGTTTGA GAACCAAACC 
CGGCTCCCCG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAACGGGGAG 
AAGGTGCTTC CCAAGGATGA CATCGAATGC CCACTGGGCT GGAAGTGGGA AGATGAGGAG 
TGGTCCACGG ACCTCAATCG GGCTGTCGAT GAACAAGGCT GGGAGTACAG CATCACCATC 
CCTCCGGACC GGAAGCCGAG GCACTGGGTC CCTGCTGAGA AGATGTACTA CACACACCGT 
CGGCGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC GCTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGTGAAGGC TGGGAGTACG CCTCACTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACAGACGCC TTTCGCCGCC GCCGCTGGCG CCGCCGCATG 
GAGCCCCTGG AGAAGACGGG ACCTGCAGCT GTGTTCGCCC TCGAGGGGGC CCTGGGCGGT 
GTGGTGGACG ACAGGAGTGA GGATTCCATG TCTGTCTCCA CCTTGAGCTT TGGCGTGAAC 
AGACCCACGA TCTCCTGCAT CTTCGACTAT GGAAACCGCT ACCATCTACG TTGCTATATG 
TACCAGGCCC GGGATCTGCC TGCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACA GTGGTGGCGA AGAATACCCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTACGAGATT GAGATCTTCG GCGAGCCAGC CAGCATCGCC 
GAGCAGCCGC CCAGCATCGT GGTGGAGCTG TATGACCATG ACACCTACGG CGCAGATGAG 
TTCATGGGAC GCTGCATCTG TCAGCCGAGT CTGGAACGGA TGCCCCGGTT GGCCTGGTTC 
CCTCTGACTA GGGGCAGCCA GCCGGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCTATCCA CCATATTCCC GGTTTTGAGG TGCAGGATAC ATCAGGGATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCC TACCCGCCAC CCCAGAGGGA GGCCAACATC 
TACATGGTCC CCCAGAACAT CAAGCCGGTT CTCCAGCGAA CAGCCATTGA GATCCTGGCA 
TGGGGGCTTC GGAACATGAA GAGTTACCAG CTGGCCAGTG TGTCTTCCCC CAGCCTGGTG 
GTGGAGTGCG GGGGCCAGAC GGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTCGACA TCTGCACCCT CTTCATGGAA GTGATGCTGC CCCGGGAGGA TCTCTACTGT 
CCCCCCATCG TGGTCAAGGT CATTGATAAC CGGCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCA TCCGCTCCCT GGAGAGCTTC CTGTGTGACC CTTACTCAGA GGAGAGTCCG 
TCCCCACAGG GCGGCCCAGA TGACGTGAGC TTGCTCAGTC CTGGGGAAGA CGTGCTGATT 
GACATCGACG ACAAGGAACC CCTCATCCCC ATCCAGCTTG CGGATGGTCT GTCGGGCTTG 
GCCCCCACTA ACATGGCGTC TTCTCCATCC AGTCCTCATG AGGAGGAGTT TATTGACTGG 
TGGAGCAAAT TCTTCGCCTC GATTGGGGAG AGGGAAAAGT GTGGGTCCTA CCTGGAGAAG 
GACTTTGACA CTCTAAAGGT CTATGATACC CCGCTGGAGA ATGTGAAGGC TTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGATGGAA 
GACCCATCTG TCATTGGGGA ATTTAAGGGC CTCTTCAAAA TTTATCCCCT CCCAGAAGAC 
CCTGCCATTC CCATGCCTCC AAGACAGTTC CACCAGCTGG CCTCCCAGGA GCCCCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTTGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CGGTGAGCGA CCAAGACAAC 
TACATCCCCT GCACCCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACCCTC TATGACTACG ACCTCCTCTC CAAGGATGAA 
AAGATCGGGG AGACGGTCAT CGACCTGGAG AACAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGCCTCC CCCAGACCTA TTGCGTCTCT GGACCCAACC AGTGGAGGGA TCAGCTCCGT 
CCCTCCCAGC TTCTCCACCT GTTCTGCCAG CAGCACAGGG TCAAGGCCCC CGTGTACCGG 
ACGGACCGCG TGGTGTTTCA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAGG 
GTCCCAAACC CACACCTGGG CCCAGCAGAG GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAACA CGTGGAGTCC CGCCCCCTCT ATAGCCCCCT GCAGCCGGAC 
ATCGAGCAGG GGAAGCTGCA GATGTGGATC GACCTGTTTC CAAAGGCCCT GGGGCGGCCG 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGGAGGT TTTTCCTGCG TTGTATTATC 
TGGAACACCA AGGATGTGAT TCTGGATGAC CTCAGCATCA CCGGGGAGAA GATGAGTGAC 
ATTTATGTGA AAGGCTGGAT GGTGGGCTTT GAAGAACACA AGCAGAAGAC AGATGTGCAT 
TATCGGTCCC TGGGAGGCGA AGGCAACTTT AACTGGAGGT TCGTTTTCCC TTTCGACTAC 
CTGCCAGCTG AGCAAGTCTG CACCGTCTCC AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AGATCCCTGC GCGAGTAGTC TTCCAGATTT GGGACAACGA CAAGTTCTCC 
TTTGATGACT TTTTGGGTGA GCACCATTTC TGGTCTGTAC CACTCTCCAC TCGGGGCTGC 
CCCAGAACCC ACGCTTTGCG GTGTTGGATG GTAAGACTCA GGGTGGTGGT GGGGGGCACA 
GGGTGGAGGC AGTGCAGGCT CCAGTGGGCC GTCACTGGCT GGCTGGCCTC TCACTTGACA 
GGATCTCTAC CACCTCAGTC GAGACAGCTT GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100522732 ( XM_013996053.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851507.1 Sus scrofa dysferlin (LOC100522732), transcript variant X16, mRNA.

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CloneID	OSe34215
Clone ID Related Accession (Same CDS sequence)	XM_013996053.1
Accession Version	XM_013996053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	dysferlin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609858.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGT GGACGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TACGTCAACC TGTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCGGAG 
CTCAACACAG GCAAGGGGGA AGGCGTGGCC TACCGAGGCC GGCTGCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGATC TTCCTGCAGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCTGCCTT CTACTCGGCC 
ACCATGCTAC AGGACGTGGA CGACGCCATC CAGTTCGAGG TCAGCATCGG GAACTACGGG 
AACAAGTTCG ACACGACCTG CCTGCCTCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACCGA GTGGAGGCTC AGAACCAGCT GCTCAGGATC 
GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTTCACTTGG CCCTGAGGGC GCAGTGCTCC 
ACCGAGGACG TGGACTCCCT GGCGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC 
CAGCCCCTAG GTGACATCCA TGAGACGCCC TCTGCTACCC ACCTGGACCA GTACCTGCAC 
CGGCTGCGTA TGCGTCACCT GAGCCAGATC GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC 
CACAGTGAAC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT GCAGGGCGAC 
AAGCGGGTGG CCTACCAGCG GGTGCCTGCC CACGAGGTCC TCTTCTCCCG GCGGGGCTCC 
AGCTACTGTG GCAAGAATTG TGGAAAGCTG CAGACGATAT TTCTGAAATA TCCGATGGAG 
GGGATGCCCA GAGCCAGGAT GCCAGTGCAG ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC 
GTGGATGAAA AGGAGTTCAA TCAGTTTGCA GAGGGCAAGC TCTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGCT GGCCCTGGTC GGAAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGCAAGATC AAGCTCCCCA AGGACAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCGG AGAAGACCCT GCTTCATGAC 
ACTGATGCGG GCCATCTGAG CTTCGTGGAG GAGGTGTTTG AGAACCAAAC CCGGCTCCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAATG CCCACTGGGC TGGAAGTGGG AAGATGAGGA GTGGTCCACG 
GACCTCAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCTGAG AAGATGTACT ACACACACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC GCCTCACTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGACGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GACCTGCAGC TGTGTTCGCC CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC 
GACAGGAGTG AGGATTCCAT GTCTGTCTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATCTCCTGCA TCTTCGACTA TGGAAACCGC TACCATCTAC GTTGCTATAT GTACCAGGCC 
CGGGATCTGC CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC AGTGGTGGCG AAGAATACCC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTACGAGAT TGAGATCTTC GGCGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTACG GCGCAGATGA GTTCATGGGA 
CGCTGCATCT GTCAGCCGAG TCTGGAACGG ATGCCCCGGT TGGCCTGGTT CCCTCTGACT 
AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCTATCC ACCATATTCC CGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC CTACCCGCCA CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACAGCCATTG AGATCCTGGC ATGGGGGCTT 
CGGAACATGA AGAGTTACCA GCTGGCCAGT GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCCGGGAGG ATCTCTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGGCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGACGTGAG CTTGCTCAGT CCTGGGGAAG ACGTGCTGAT TGACATCGAC 
GACAAGGAAC CCCTCATCCC CATCCAGGAG GAGGAGTTTA TTGACTGGTG GAGCAAATTC 
TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT GGGTCCTACC TGGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGATACCCC GCTGGAGAAT GTGAAGGCTT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC 
ATTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC TGCCATTCCC 
ATGCCTCCAA GACAGTTCCA CCAGCTGGCC TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGCGACC AAGACAACTA CATCCCCTGC 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACCCTCTA TGACTACGAC CTCCTCTCCA AGGATGAAAA GATCGGGGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC 
CAGACCTATT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGTCC CTCCCAGCTT 
CTCCACCTGT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCCAAACCCA 
CACCTGGGCC CAGCAGAGGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAACACG TGGAGTCCCG CCCCCTCTAT AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGGAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATTC TGGATGACCT CAGCATCACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
GGCTGGATGG TGGGCTTTGA AGAACACAAG CAGAAGACAG ATGTGCATTA TCGGTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC GTTTTCCCTT TCGACTACCT GCCAGCTGAG 
CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAG 
ATCCCTGCGC GAGTAGTCTT CCAGATTTGG GACAACGACA AGTTCTCCTT TGATGACTTT 
TTGGGTGAGC ACCATTTCTG GTCTGTACCA CTCTCCACTC GGGGCTGCCC CAGAACCCAC 
GCTTTGCGGT GTTGGATGGT AAGACTCAGG GTGGTGGTGG GGGGCACAGG GTGGAGGCAG 
TGCAGGCTCC AGTGGGCCGT CACTGGCTGG CTGGCCTCTC ACTTGACAGG ATCTCTACCA 
CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851507.1
CDS	526..6552
Translation	MLRVFILYAENVHTQDSDISDAYCSAVFAGVKKRTKVIKNNVNPVWNEGFEWDLKGVPLDQSSELHVVVKDHETMGRNRFLGEANVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPVFPPATPLEPSPTLPDMDMVAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPFPDQSGTPGPGAPITPKKPPSHPPHHRPGGRRRRSTPAPRKPLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPAELFNEPIFITVVDSRSLRTDALIGEFRMDVGSIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIEGNLLRPTGVALRGANFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFMEVSFAGKMLCGKILEKTANPQWNQSITLPAMFPSMCEKMRIRVMDWDRLTHNDIVATTYLSMSKISAPGGEVEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALRAQCSTEDVDSLAAQLMDELIADCSQPLGDIHETPSATHLDQYLHRLRMRHLSQIVEAAQALKLGHSELPAALEQAEDWLLRLRALADEPQNSLPDIVIWMLQGDKRVAYQRVPAHEVLFSRRGSSYCGKNCGKLQTIFLKYPMEGMPRARMPVQIRVRLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDTDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPRHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPAMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASVSSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTPLENVKAFEGLSDFCNTFKLYRGKTQEEMEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLASQEPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVVFQDKEYTIEEIEAGRVPNPHLGPAEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFVFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGEHHFWSVPLSTRGCPRTHALRCWMVRLRVVVGGTGWRQCRLQWAVTGWLASHLTGSLPPQSRQLGQ

Target ORF information:

RefSeq Version	XM_013996053.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa dysferlin (LOC100522732), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013996053.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCGGAG AACGTCCACA CCCAGGACTC CGACATCAGC 
GATGCCTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AACGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCGT CCCCCTGGAC 
CAGAGCTCTG AGCTTCATGT GGTGGTCAAA GACCACGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAACGTCCC ACTCCGGGAG GTTCTCGCCA CCCCCAGCCT GTCCGCCAGC 
TTCAATGCCC CACTGCTGGA CACTAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CGCCACTTCC TGGAGCTGTG CCTGTGTTCC CACCCGCTAC ACCTCTGGAA 
CCCTCCCCGA CTCTGCCCGA CATGGATATG GTGGCTGGTG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACGGAGGACC AGGGGCTGAC GGGAGACGAG 
GCCGAGCCAT TCCCGGACCA GAGCGGAACT CCGGGTCCAG GGGCCCCCAT CACCCCAAAG 
AAGCCACCTT CCCATCCTCC CCACCACCGA CCAGGGGGGA GAAGGAGGAG AAGCACGCCC 
GCGCCCAGAA AGCCGCTGTC GGACAAACCT CAGGACTTCC AGATCAGGGT CCAGGTGATC 
GAGGGACGCC AGCTGCCGGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC CGCTGCCGGG 
CAGACCAAGC GGACTCGGAT CCACAAGGGA AACAGCCCCC TCTTCAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TGCGGAGCTG TTTAATGAGC CCATCTTCAT CACGGTGGTG 
GACTCCCGTT CCCTCAGGAC AGACGCTCTC ATCGGGGAGT TCCGGATGGA CGTGGGCAGC 
ATCTACCGGG AACCCCGACA CGCCTATCTC AGGAAGTGGC TGCTGCTCTC GGACCCAGAC 
GACTTCTCTG CTGGGGCCAG AGGCTACCTG AAAGCCAGCC TTTGCGTGCT GGGCCCTGGA 
GACGAGGCCC CTCTGGAGAG GAAAGACCCC TCTGAGGACA AGGAGGACAT CGAAGGCAAC 
CTGCTCAGGC CGACGGGCGT GGCCCTCCGT GGAGCGAACT TCTGCCTGAA GGTCTTCAGG 
GCGGAGGACT TACCGCAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGG 
TTTGAGAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTATGTG GCAAGATCCT GGAGAAGACG GCCAACCCTC AGTGGAACCA GAGTATCACG 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAG AAGATGAGGA TTCGTGTCAT GGACTGGGAC 
CGCCTCACCC ACAACGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAGTAGAAGT GGACGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TACGTCAACC TGTACGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCGGAG 
CTCAACACAG GCAAGGGGGA AGGCGTGGCC TACCGAGGCC GGCTGCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGATC TTCCTGCAGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TACTCGCTCT TTGCTGCCTT CTACTCGGCC 
ACCATGCTAC AGGACGTGGA CGACGCCATC CAGTTCGAGG TCAGCATCGG GAACTACGGG 
AACAAGTTCG ACACGACCTG CCTGCCTCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGAT GCCACTACTA CTACTTACCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCCTACT GGGAGGACAT CAGCCACCGA GTGGAGGCTC AGAACCAGCT GCTCAGGATC 
GCCGACCGGC TGGAAGCCGG CCTGGAGCAG GTTCACTTGG CCCTGAGGGC GCAGTGCTCC 
ACCGAGGACG TGGACTCCCT GGCGGCTCAG CTGATGGATG AGCTCATTGC AGACTGCAGC 
CAGCCCCTAG GTGACATCCA TGAGACGCCC TCTGCTACCC ACCTGGACCA GTACCTGCAC 
CGGCTGCGTA TGCGTCACCT GAGCCAGATC GTTGAGGCGG CCCAGGCCCT GAAGCTTGGC 
CACAGTGAAC TCCCCGCTGC CCTGGAGCAG GCTGAGGACT GGCTCCTGCG TCTCCGTGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCT GACATCGTCA TCTGGATGCT GCAGGGCGAC 
AAGCGGGTGG CCTACCAGCG GGTGCCTGCC CACGAGGTCC TCTTCTCCCG GCGGGGCTCC 
AGCTACTGTG GCAAGAATTG TGGAAAGCTG CAGACGATAT TTCTGAAATA TCCGATGGAG 
GGGATGCCCA GAGCCAGGAT GCCAGTGCAG ATTCGGGTCA GGCTGTGGTT CGGGCTCTCC 
GTGGATGAAA AGGAGTTCAA TCAGTTTGCA GAGGGCAAGC TCTCTGTCTT TGCTGAAACC 
TATGAGAACC AGACCAAGCT GGCCCTGGTC GGAAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGCAAGATC AAGCTCCCCA AGGACAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCGG AGAAGACCCT GCTTCATGAC 
ACTGATGCGG GCCATCTGAG CTTCGTGGAG GAGGTGTTTG AGAACCAAAC CCGGCTCCCC 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAACGGGGA GAAGGTGCTT 
CCCAAGGATG ACATCGAATG CCCACTGGGC TGGAAGTGGG AAGATGAGGA GTGGTCCACG 
GACCTCAATC GGGCTGTCGA TGAACAAGGC TGGGAGTACA GCATCACCAT CCCTCCGGAC 
CGGAAGCCGA GGCACTGGGT CCCTGCTGAG AAGATGTACT ACACACACCG TCGGCGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CGCTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGTGAAGG CTGGGAGTAC GCCTCACTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACAGACGC CTTTCGCCGC CGCCGCTGGC GCCGCCGCAT GGAGCCCCTG 
GAGAAGACGG GACCTGCAGC TGTGTTCGCC CTCGAGGGGG CCCTGGGCGG TGTGGTGGAC 
GACAGGAGTG AGGATTCCAT GTCTGTCTCC ACCTTGAGCT TTGGCGTGAA CAGACCCACG 
ATCTCCTGCA TCTTCGACTA TGGAAACCGC TACCATCTAC GTTGCTATAT GTACCAGGCC 
CGGGATCTGC CTGCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC AGTGGTGGCG AAGAATACCC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTACGAGAT TGAGATCTTC GGCGAGCCAG CCAGCATCGC CGAGCAGCCG 
CCCAGCATCG TGGTGGAGCT GTATGACCAT GACACCTACG GCGCAGATGA GTTCATGGGA 
CGCTGCATCT GTCAGCCGAG TCTGGAACGG ATGCCCCGGT TGGCCTGGTT CCCTCTGACT 
AGGGGCAGCC AGCCGGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCTATCC ACCATATTCC CGGTTTTGAG GTGCAGGATA CATCAGGGAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC CTACCCGCCA CCCCAGAGGG AGGCCAACAT CTACATGGTC 
CCCCAGAACA TCAAGCCGGT TCTCCAGCGA ACAGCCATTG AGATCCTGGC ATGGGGGCTT 
CGGAACATGA AGAGTTACCA GCTGGCCAGT GTGTCTTCCC CCAGCCTGGT GGTGGAGTGC 
GGGGGCCAGA CGGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTCGAC 
ATCTGCACCC TCTTCATGGA AGTGATGCTG CCCCGGGAGG ATCTCTACTG TCCCCCCATC 
GTGGTCAAGG TCATTGATAA CCGGCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
ATCCGCTCCC TGGAGAGCTT CCTGTGTGAC CCTTACTCAG AGGAGAGTCC GTCCCCACAG 
GGCGGCCCAG ATGACGTGAG CTTGCTCAGT CCTGGGGAAG ACGTGCTGAT TGACATCGAC 
GACAAGGAAC CCCTCATCCC CATCCAGGAG GAGGAGTTTA TTGACTGGTG GAGCAAATTC 
TTCGCCTCGA TTGGGGAGAG GGAAAAGTGT GGGTCCTACC TGGAGAAGGA CTTTGACACT 
CTAAAGGTCT ATGATACCCC GCTGGAGAAT GTGAAGGCTT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGATGGAAGA CCCATCTGTC 
ATTGGGGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC TGCCATTCCC 
ATGCCTCCAA GACAGTTCCA CCAGCTGGCC TCCCAGGAGC CCCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCG GTGAGCGACC AAGACAACTA CATCCCCTGC 
ACCCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACCCTCTA TGACTACGAC CTCCTCTCCA AGGATGAAAA GATCGGGGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGCCTCCCC 
CAGACCTATT GCGTCTCTGG ACCCAACCAG TGGAGGGATC AGCTCCGTCC CTCCCAGCTT 
CTCCACCTGT TCTGCCAGCA GCACAGGGTC AAGGCCCCCG TGTACCGGAC GGACCGCGTG 
GTGTTTCAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAGGGT CCCAAACCCA 
CACCTGGGCC CAGCAGAGGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAACACG TGGAGTCCCG CCCCCTCTAT AGCCCCCTGC AGCCGGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGATCGA CCTGTTTCCA AAGGCCCTGG GGCGGCCGGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGGAGGTTT TTCCTGCGTT GTATTATCTG GAACACCAAG 
GATGTGATTC TGGATGACCT CAGCATCACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
GGCTGGATGG TGGGCTTTGA AGAACACAAG CAGAAGACAG ATGTGCATTA TCGGTCCCTG 
GGAGGCGAAG GCAACTTTAA CTGGAGGTTC GTTTTCCCTT TCGACTACCT GCCAGCTGAG 
CAAGTCTGCA CCGTCTCCAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAG 
ATCCCTGCGC GAGTAGTCTT CCAGATTTGG GACAACGACA AGTTCTCCTT TGATGACTTT 
TTGGGTGAGC ACCATTTCTG GTCTGTACCA CTCTCCACTC GGGGCTGCCC CAGAACCCAC 
GCTTTGCGGT GTTGGATGGT AAGACTCAGG GTGGTGGTGG GGGGCACAGG GTGGAGGCAG 
TGCAGGCTCC AGTGGGCCGT CACTGGCTGG CTGGCCTCTC ACTTGACAGG ATCTCTACCA 
CCTCAGTCGA GACAGCTTGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC100522732
Entrez Gene ID	100522732
Full Name	dysferlin
Gene Type	protein-coding
Organism	Sus scrofa(Pig)

LOC100522732 cDNA ORF clone, Sus scrofa(Pig)

Gene

Clones

gRNAs

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