PCCB cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCCB
Entrez Gene ID	100158147
Full Name	propionyl-CoA carboxylase beta subunit
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 13 Map Location: 13\|13

mRNA and Protein(s)

mRNA	Protein	Name
NM_213901.1	NP_999066.1	propionyl-CoA carboxylase beta chain, mitochondrial precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
J?rgensen FG, Hobolth A, Hornsh?j H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Metabolism of propionic acid in animal tissues. VIII. Crystalline propionyl carboxylase.
KAZIRO Y, OCHOA S, WARNER RC, CHEN JY
The Journal of biological chemistry2361917-23(1961 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PCCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00278	NM_213901.1 Latest version!	Sus scrofa propionyl-CoA carboxylase subunit beta (PCCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCCB ( NM_213901.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999066.1 Sus scrofa propionyl-CoA carboxylase subunit beta (PCCB), mRNA.

Price & Availability↑

CloneID	OSe00278
Clone ID Related Accession (Same CDS sequence)	NM_213901.1
Accession Version	NM_213901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Sus scrofa(pig)
Product	propionyl-CoA carboxylase beta chain, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB000886.1. On Mar 29, 2010 this sequence version replaced XM_001925696.1. ##Evidence-Data-START## Transcript exon combination :: AB000886.1, AY609485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGG CAGTGCGCGT GACGGCGGCC CGAGCCAGGC TCCGCGTTGT AGTGAGAAGT 
CTCCACGCCG GGGTGCGCAG TCTGTGCACC CAGCCTGTCT CAGTTAACGA GCGCATCGAG 
AACAAGCGTC AGGCCGCACT GCTGGGAGGA GGCCAGCGCC GCATTGATTC GCAGCATAAG 
CGAGGAAAGC TAACGGCCAG AGAACGGATC AGTCTCTTGC TGGACCCCGG CAGTTTTATT 
GAGAGTGACA TGTTTGTGGA ACACAGATGT GCAGATTTTG GAATGGCTGC TGACAAGAAT 
AAGTTTCCTG GAGACAGCGT TGTCACTGGA CGGGGCCGAA TCAATGGAAG ATTGGTTTAT 
GTCTTCAGTC AGGATTTTAC CGTTTTTGGA GGCAGTCTGT CTGGAGCCCA TGCCCAAAAG 
ATCTGCAAGA TCATGGACCA AGCCATGACA GTTGGGGCTC CGGTGATTGG ACTGAATGAC 
TCAGGGGGAG CCAGGATCCA AGAAGGAGTA GAGTCTTTGG CTGGCTATGC AGACATCTTT 
CTGAGGAATG TCTCGGCATC TGGAGTCATC CCTCAGATTT CTCTGATCAT GGGCCCGTGT 
GCTGGTGGGG CTGTCTACTC CCCGGCCCTG ACAGACTTCA CATTCATGGT AAAGGACACC 
TCCTACTTAT TCATCACTGG TCCTGATGTT GTGAAGTCTG TCACCAACGA GGACGTGACC 
CAGGAGGAGC TCGGGGGTGC CAGGACCCAC ACCACCATGT CAGGCGTGGC CCACAGAGCT 
TTTGACAATG ATGTCGATGC CCTATGTAAT CTCCGGGAGT TCTTCAACTA CCTGCCTCTC 
AGCAATCAGG ACCCGGCTCC CATCCGTGAG TGCCATGATC CCAGTGACCG TCTGGTTCCT 
GAGCTTGACA CAGTTGTCCC TTTGGAATCG ACTAGAGCCT ATGACATGGT GGACATAATA 
TACTCTATTG TTGACGAGCG AGACTTTTTT GAGATCATGC CCAATTATGC CAAGAACATC 
ATTGTTGGTT TTGCAAGAAT GAATGGGAGG ACTGTTGGAA TTGTTGGCAA CCAGCCTAAG 
GTGGCTTCAG GATGCTTGGA TATTAATTCA TCTGTGAAAG GGGCTCGTTT TGTCCGATTC 
TGTGATGCAT TTAACATTCC ACTCATCACT TTCGTTGATG TCCCTGGCTT TCTGCCTGGC 
ACAGCGCAGG AATACGGGGG CATCATCCGG CATGGCGCCA AGCTGCTCTA TGCGTTCGCC 
GAGGCAACTG TGCCCAAAAT CACAGTCATC ACCAGGAAGG CCTATGGTGG AGCCTATGAT 
GTCATGAGCT CCAAGCACCT TTGTGGTGAC ACCAACTACG CCTGGCCCAC AGCAGAGATT 
GCCGTCATGG GAGCAAAGGG TGCCGTGGAG ATCATCTTCA AAGGGCACGA GAATGTGGAG 
GCCGCGCAGG CAGAGTACAT TGAGAAGTTT GCCAACCCTT TTCCTGCAGC TGTGAGAGGG 
TTTGTGGATG ACATCATCCA GCCTTCCTCT ACCCGGGCCC GAATCTGCTG TGACCTGGAT 
GTCTTGGCCA GCAAGAAGGT ACAGCGCCCT TGGAGGAAAC ATGCAAATAT TCCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999066.1
CDS	11..1630
Translation	MAAAVRVTAARARLRVVVRSLHAGVRSLCTQPVSVNERIENKRQAALLGGGQRRIDSQHKRGKLTARERISLLLDPGSFIESDMFVEHRCADFGMAADKNKFPGDSVVTGRGRINGRLVYVFSQDFTVFGGSLSGAHAQKICKIMDQAMTVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVSASGVIPQISLIMGPCAGGAVYSPALTDFTFMVKDTSYLFITGPDVVKSVTNEDVTQEELGGARTHTTMSGVAHRAFDNDVDALCNLREFFNYLPLSNQDPAPIRECHDPSDRLVPELDTVVPLESTRAYDMVDIIYSIVDERDFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKITVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL

Target ORF information:

RefSeq Version	NM_213901.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa propionyl-CoA carboxylase subunit beta (PCCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213901.1

ORF Insert Sequence:

ATGGCGGCGG CAGTGCGCGT GACGGCGGCC CGAGCCAGGC TCCGCGTTGT AGTGAGAAGT 
CTCCACGCCG GGGTGCGCAG TCTGTGCACC CAGCCTGTCT CAGTTAACGA GCGCATCGAG 
AACAAGCGTC AGGCCGCACT GCTGGGAGGA GGCCAGCGCC GCATTGATTC GCAGCATAAG 
CGAGGAAAGC TAACGGCCAG AGAACGGATC AGTCTCTTGC TGGACCCCGG CAGTTTTATT 
GAGAGTGACA TGTTTGTGGA ACACAGATGT GCAGATTTTG GAATGGCTGC TGACAAGAAT 
AAGTTTCCTG GAGACAGCGT TGTCACTGGA CGGGGCCGAA TCAATGGAAG ATTGGTTTAT 
GTCTTCAGTC AGGATTTTAC CGTTTTTGGA GGCAGTCTGT CTGGAGCCCA TGCCCAAAAG 
ATCTGCAAGA TCATGGACCA AGCCATGACA GTTGGGGCTC CGGTGATTGG ACTGAATGAC 
TCAGGGGGAG CCAGGATCCA AGAAGGAGTA GAGTCTTTGG CTGGCTATGC AGACATCTTT 
CTGAGGAATG TCTCGGCATC TGGAGTCATC CCTCAGATTT CTCTGATCAT GGGCCCGTGT 
GCTGGTGGGG CTGTCTACTC CCCGGCCCTG ACAGACTTCA CATTCATGGT AAAGGACACC 
TCCTACTTAT TCATCACTGG TCCTGATGTT GTGAAGTCTG TCACCAACGA GGACGTGACC 
CAGGAGGAGC TCGGGGGTGC CAGGACCCAC ACCACCATGT CAGGCGTGGC CCACAGAGCT 
TTTGACAATG ATGTCGATGC CCTATGTAAT CTCCGGGAGT TCTTCAACTA CCTGCCTCTC 
AGCAATCAGG ACCCGGCTCC CATCCGTGAG TGCCATGATC CCAGTGACCG TCTGGTTCCT 
GAGCTTGACA CAGTTGTCCC TTTGGAATCG ACTAGAGCCT ATGACATGGT GGACATAATA 
TACTCTATTG TTGACGAGCG AGACTTTTTT GAGATCATGC CCAATTATGC CAAGAACATC 
ATTGTTGGTT TTGCAAGAAT GAATGGGAGG ACTGTTGGAA TTGTTGGCAA CCAGCCTAAG 
GTGGCTTCAG GATGCTTGGA TATTAATTCA TCTGTGAAAG GGGCTCGTTT TGTCCGATTC 
TGTGATGCAT TTAACATTCC ACTCATCACT TTCGTTGATG TCCCTGGCTT TCTGCCTGGC 
ACAGCGCAGG AATACGGGGG CATCATCCGG CATGGCGCCA AGCTGCTCTA TGCGTTCGCC 
GAGGCAACTG TGCCCAAAAT CACAGTCATC ACCAGGAAGG CCTATGGTGG AGCCTATGAT 
GTCATGAGCT CCAAGCACCT TTGTGGTGAC ACCAACTACG CCTGGCCCAC AGCAGAGATT 
GCCGTCATGG GAGCAAAGGG TGCCGTGGAG ATCATCTTCA AAGGGCACGA GAATGTGGAG 
GCCGCGCAGG CAGAGTACAT TGAGAAGTTT GCCAACCCTT TTCCTGCAGC TGTGAGAGGG 
TTTGTGGATG ACATCATCCA GCCTTCCTCT ACCCGGGCCC GAATCTGCTG TGACCTGGAT 
GTCTTGGCCA GCAAGAAGGT ACAGCGCCCT TGGAGGAAAC ATGCAAATAT TCCCTTGTAA