PCK1 cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK1
Entrez Gene ID	104150016
Full Name	phosphoenolpyruvate carboxykinase 1
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009683988.1	XP_009682283.1	phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Pathways from BioSystems
Gene Ontology

The following PCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt79864	XM_009683988.1 Latest version!	Struthio camelus australis phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK1 ( XM_009683988.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009682283.1 Struthio camelus australis phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mRNA.

Price & Availability↑

CloneID	OSt79864
Clone ID Related Accession (Same CDS sequence)	XM_009683988.1
Accession Version	XM_009683988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009271896.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCCAC AGTTGAAGAC CGAGCTAAAC ATCATCCCCA AGGTTATCCA GGGGGATTTG 
CAGAGCCTGC CTCCAGAAGT GAGAGAGTTC ATTGAAAGCA ATGCCAAGCT GTGCCAGCCT 
GAGAGCATTC ATATCTGTGA TGGCTCAGAA GAAGAAAACC AAAAAATTCT GGACATCATG 
GTAGAACAAG GCATGATTAA GAAGCTGAGC AAGTATGAGA ACTGCTGGTT GGCTCTCACT 
GATCCAAGAG ATGTGGCAAG AATTGAGAGC AAAACTGTCA TTATCACTCC AGAACAGAGA 
GATACCACTC CAATCCCTAA AAACGGAACT AGCCAGCTGG GCCGCTGGAT GTCAGAAGAC 
GATTTTGAGA AAGCCTTCAA TACCAGATTC CCGGGCTGCA TGCAAGGACG TACAATGTAT 
GTCATCCCCT TCAGCATGGG GCCTATTGGG TCTCCTTTAT CCAAGACTGG GATTGAATTG 
ACAGATTCAC CGTATGTAGT GGCCAGCATG AGGATCATGA CACGAATGGG AACAGCTGCT 
TTGAAAGCCC TGGGCAATGG AGAGTTTGTA AAATGCCTTC ACTCAGTTGG ATGCCCTCTG 
CCGCTGAAAG AACCGCTAAT CAACAACTGG CCATGCAACC CCGAGTTAAC ACTGATTGCT 
CATCTCCCAG ATCGCAGAGA GATCATTTCA TTTGGCAGCG GCTACGGAGG AAACTCCTTA 
CTGGGGAAAA AATGCTTTGC TCTCAGAATC GCCAGCAGAA TCGCCAAGGA AGAGGGCTGG 
CTAGCTGAGC ACATGCTGAT CCTGGGCATT ACAAATCCAG AAGGTGACAA GAAGTATTTT 
GCTGCAGCAT TCCCTAGCGC ATGTGGAAAG ACAAACTTGG CCATGATGAA CCCAAGTTTG 
CCAGGATGGA AAATCGAGTG TGTGGGGGAC GATATTGCCT GGATGAAATT TGATGAACAA 
GGCAACTTAC GAGCAATCAA TCCAGAAAAT GGTTTTTTTG GCGTTGCCCC TGGAACTTCG 
GTCAAAACAA ACCCCAATGC CATTAAAACC ATATTCAAGA ACACCATCTT TACTAACGTA 
GCTGAAACCA GTGATGGAGG TATCTACTGG GAAGGCATTG ATGAGCCATT GCCATCAGGA 
GTAACACTGA CTTCATGGAA GAACAAGAAC TGGACCCCAG ATAATGGGGA ACCTTGTGCT 
CATCCCAATT CACGATTCTG CACCCCAGCC AGTCAGTGCC CCATCATAGA CCCTGCATGG 
GACTCACCAG AAGGCGTGCC TATTGAAGCC ATCATATTTG GAGGGCGCAG ACCTACTGGT 
GTACCTCTTG TGTATGAGGC CTTTAACTGG CAGCACGGAG TATTTATAGG AGCAGCTATG 
AGATCTGAAG CAACAGCAGC TGCTGAGCAC AAAGGCAAAA TCATTATGCA TGACCCATTT 
GCCATGAGAC CTTTCTTTGG CTATAACTTT GGCAAATACT TAGCCCACTG GCTCAGCATG 
GCACATCGTC CAGCCGCGAA ACTACCAAAG ATCTTCCATG TTAACTGGTT CAGGAAAGAC 
AACCAAGGGA AATTCCTGTG GCCTGGCTAC GGAGAGAATT CCCGTGTGCT GGAGTGGATG 
TTCAACAGAA TTCAAGGGAA AGCCTCTGCC AAGCCAACTG CCATAGGTTA CATCCCTGCT 
GACACGGCTT TGAACCTGAA AGGTTTAGAA AATATCAGCT TGACTGAACT GTTTGAAGTC 
TCCAAAGAGT TCTGGGAAAA GGAGGTGGAA GAAATCAAAC AATACTTTGA AGGGCAGATT 
AATGCTGACC TTCCCTATGA AATAGAAAGA GAAGTGCTTG CCTTGGAGAA GAGGATAAAA 
CAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009682283.1
CDS	153..2021
Translation	MPPQLKTELNIIPKVIQGDLQSLPPEVREFIESNAKLCQPESIHICDGSEEENQKILDIMVEQGMIKKLSKYENCWLALTDPRDVARIESKTVIITPEQRDTTPIPKNGTSQLGRWMSEDDFEKAFNTRFPGCMQGRTMYVIPFSMGPIGSPLSKTGIELTDSPYVVASMRIMTRMGTAALKALGNGEFVKCLHSVGCPLPLKEPLINNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRIAKEEGWLAEHMLILGITNPEGDKKYFAAAFPSACGKTNLAMMNPSLPGWKIECVGDDIAWMKFDEQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIFKNTIFTNVAETSDGGIYWEGIDEPLPSGVTLTSWKNKNWTPDNGEPCAHPNSRFCTPASQCPIIDPAWDSPEGVPIEAIIFGGRRPTGVPLVYEAFNWQHGVFIGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDNQGKFLWPGYGENSRVLEWMFNRIQGKASAKPTAIGYIPADTALNLKGLENISLTELFEVSKEFWEKEVEEIKQYFEGQINADLPYEIEREVLALEKRIKQL

Target ORF information:

RefSeq Version	XM_009683988.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009683988.1

ORF Insert Sequence:

ATGCCCCCAC AGTTGAAGAC CGAGCTAAAC ATCATCCCCA AGGTTATCCA GGGGGATTTG 
CAGAGCCTGC CTCCAGAAGT GAGAGAGTTC ATTGAAAGCA ATGCCAAGCT GTGCCAGCCT 
GAGAGCATTC ATATCTGTGA TGGCTCAGAA GAAGAAAACC AAAAAATTCT GGACATCATG 
GTAGAACAAG GCATGATTAA GAAGCTGAGC AAGTATGAGA ACTGCTGGTT GGCTCTCACT 
GATCCAAGAG ATGTGGCAAG AATTGAGAGC AAAACTGTCA TTATCACTCC AGAACAGAGA 
GATACCACTC CAATCCCTAA AAACGGAACT AGCCAGCTGG GCCGCTGGAT GTCAGAAGAC 
GATTTTGAGA AAGCCTTCAA TACCAGATTC CCGGGCTGCA TGCAAGGACG TACAATGTAT 
GTCATCCCCT TCAGCATGGG GCCTATTGGG TCTCCTTTAT CCAAGACTGG GATTGAATTG 
ACAGATTCAC CGTATGTAGT GGCCAGCATG AGGATCATGA CACGAATGGG AACAGCTGCT 
TTGAAAGCCC TGGGCAATGG AGAGTTTGTA AAATGCCTTC ACTCAGTTGG ATGCCCTCTG 
CCGCTGAAAG AACCGCTAAT CAACAACTGG CCATGCAACC CCGAGTTAAC ACTGATTGCT 
CATCTCCCAG ATCGCAGAGA GATCATTTCA TTTGGCAGCG GCTACGGAGG AAACTCCTTA 
CTGGGGAAAA AATGCTTTGC TCTCAGAATC GCCAGCAGAA TCGCCAAGGA AGAGGGCTGG 
CTAGCTGAGC ACATGCTGAT CCTGGGCATT ACAAATCCAG AAGGTGACAA GAAGTATTTT 
GCTGCAGCAT TCCCTAGCGC ATGTGGAAAG ACAAACTTGG CCATGATGAA CCCAAGTTTG 
CCAGGATGGA AAATCGAGTG TGTGGGGGAC GATATTGCCT GGATGAAATT TGATGAACAA 
GGCAACTTAC GAGCAATCAA TCCAGAAAAT GGTTTTTTTG GCGTTGCCCC TGGAACTTCG 
GTCAAAACAA ACCCCAATGC CATTAAAACC ATATTCAAGA ACACCATCTT TACTAACGTA 
GCTGAAACCA GTGATGGAGG TATCTACTGG GAAGGCATTG ATGAGCCATT GCCATCAGGA 
GTAACACTGA CTTCATGGAA GAACAAGAAC TGGACCCCAG ATAATGGGGA ACCTTGTGCT 
CATCCCAATT CACGATTCTG CACCCCAGCC AGTCAGTGCC CCATCATAGA CCCTGCATGG 
GACTCACCAG AAGGCGTGCC TATTGAAGCC ATCATATTTG GAGGGCGCAG ACCTACTGGT 
GTACCTCTTG TGTATGAGGC CTTTAACTGG CAGCACGGAG TATTTATAGG AGCAGCTATG 
AGATCTGAAG CAACAGCAGC TGCTGAGCAC AAAGGCAAAA TCATTATGCA TGACCCATTT 
GCCATGAGAC CTTTCTTTGG CTATAACTTT GGCAAATACT TAGCCCACTG GCTCAGCATG 
GCACATCGTC CAGCCGCGAA ACTACCAAAG ATCTTCCATG TTAACTGGTT CAGGAAAGAC 
AACCAAGGGA AATTCCTGTG GCCTGGCTAC GGAGAGAATT CCCGTGTGCT GGAGTGGATG 
TTCAACAGAA TTCAAGGGAA AGCCTCTGCC AAGCCAACTG CCATAGGTTA CATCCCTGCT 
GACACGGCTT TGAACCTGAA AGGTTTAGAA AATATCAGCT TGACTGAACT GTTTGAAGTC 
TCCAAAGAGT TCTGGGAAAA GGAGGTGGAA GAAATCAAAC AATACTTTGA AGGGCAGATT 
AATGCTGACC TTCCCTATGA AATAGAAAGA GAAGTGCTTG CCTTGGAGAA GAGGATAAAA 
CAGCTGTAG