NGEF cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	NGEF
Entrez Gene ID	104140769
Full Name	neuronal guanine nucleotide exchange factor
Synonyms	Ephexin-1
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009669730.1	XP_009668025.1	ephexin-1

Pathways from BioSystems
Gene Ontology

The following NGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt69625	XM_009669730.1 Latest version!	Struthio camelus australis neuronal guanine nucleotide exchange factor (NGEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGEF ( XM_009669730.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009668025.1 Struthio camelus australis neuronal guanine nucleotide exchange factor (NGEF), mRNA.

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CloneID	OSt69625
Clone ID Related Accession (Same CDS sequence)	XM_009669730.1
Accession Version	XM_009669730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	ephexin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009270783.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAACA AATACCCATT CCCACAATGC ATTCCTACTT TTTTTGTTCA GTGTAACCTC 
TCAGATCTGT TATATTGCCT TCTAGGGCTC TTGTATCAGG AATATCGAGA TAAATCCACG 
CGCCAGGAGA TTGAAACCAG GCGGCTGCAG GATTCACAGA CAGACCCTGA AGAGAATTCA 
GCCAGCGAGG AAACTCCATC TGAAACAGAA GCTGCAAGTA CAACTGAAAG CAAAGTGGCA 
CCTCAAATCA GTTTGCTGAG GAACTCCACC TCCCGGTTCA ACTTATGGCA GGATCTTCCT 
GAGATCCGGA GCAGTGGGGT CCTTAGCATC CTCCAGCCAG ATGAGATCAA GCTTCAGGAG 
GCCATGTTTG AACTGGTTAC TTCTGAAGCC TCGTATTACA AGAGTCTGAA CCTGCTGGTG 
TCTCACTTCA TGGAAAACGA ACGCCTGAAA AAGATCTTAC ATCAGTCTGA GGCACACATC 
CTCTTCTCCA ATGTGCTGGA TGTCATGGCT GTTAGTGAGC GGTTCCTGTT GGACCTTGAG 
CAGCGGGTGG AGGAGAATAT TGTGATCTCA GACGTGTGTG ACATTGTCTA CCAGCATGCG 
GTTAATCACT TCTCCGTGTA CATCACCTAC GTCTCCAACC AGACCTACCA GGAGAGAGCT 
TACAAGCAGC TTCTCCAGGA CAAGCCAGCT TTCCGGGAAG TGATATCACA GCTGGAGCTG 
GATCCCAAGT GCAAAGGCCT GTCCTTTTCT TCCTTCCTGA TTTTGCCATT TCAAAGGATC 
ACTCGACTCA AGCTGCTGGT GCAGAACATT TTGAAGAAAG TGGAAGAGAA GTCCGAGAGG 
GAAACCACTG CCCTGGATGC ACATAAGGAG CTGGAAACAG TGGTGAAAGC ATGTAATGAA 
GGTGTCAGAA AGATGAGCCG AACAGAGCAG ATGATCAGCA TCCAGAAGAA ACTGGAATTT 
AAGATCAAGT CCGTGCCCAT CATCTCGCAC TCACGCTGGC TCCTGAAGCA GGGGGAACTG 
CAGCAAATGA ACGGTCCAAA GACATCACGT ACGCTTCGTA CCAAGAAACT CTTCAGAGAA 
ATCTACCTGT TCCTTTTCAA TGACCTGCTG GTAATTTGCA GGCAAATTCC TGGGGACAAG 
TACCAAGTGT TCGACTCAGC ACCCCGGGGA CTCCTTCGGG TGGAAGAGTT AGAAGACCAG 
GGGCAGAGTT TGGCCAACGT CTTCATCTTG AGGCTGCTGG AGAACGCAGA CGACCGGGAG 
GTCAGCTACA TGCTGAAGGC GTCGTCCCAG AGCGAGATGA AGCGCTGGAT GATTTCGTTA 
GCCCCGAACC GGAGAACCAA GTTCGTTTCG TTTACATCCA GACTCGTCGA CTGCCCGCAG 
ATCCAGTGCG TCCACCCGTA CGTGGCCCAG CAGCCGGACG AGCTGTCCTT GGAGCTGGCG 
GACGTCCTCA ACATCCTGGA CAAGACAGAT GACGGTTGGA TATTTGGGGA GCGTCTTCAC 
GACCAGGAGA GGGGCTGGTT CCCCAGTTCT ATGGGGGAGG AGATCCTGAA CCCCAAAATC 
CGAGCCCAGA ACTTGAAGGA GTGCTTCCGG GTGCACAAGT CGGATGACAG CCAGCGGAGG 
AAACTGGGCA GCAGAAATCG CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009668025.1
CDS	1..1647
Translation	MQNKYPFPQCIPTFFVQCNLSDLLYCLLGLLYQEYRDKSTRQEIETRRLQDSQTDPEENSASEETPSETEAASTTESKVAPQISLLRNSTSRFNLWQDLPEIRSSGVLSILQPDEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERLKKILHQSEAHILFSNVLDVMAVSERFLLDLEQRVEENIVISDVCDIVYQHAVNHFSVYITYVSNQTYQERAYKQLLQDKPAFREVISQLELDPKCKGLSFSSFLILPFQRITRLKLLVQNILKKVEEKSERETTALDAHKELETVVKACNEGVRKMSRTEQMISIQKKLEFKIKSVPIISHSRWLLKQGELQQMNGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQSLANVFILRLLENADDREVSYMLKASSQSEMKRWMISLAPNRRTKFVSFTSRLVDCPQIQCVHPYVAQQPDELSLELADVLNILDKTDDGWIFGERLHDQERGWFPSSMGEEILNPKIRAQNLKECFRVHKSDDSQRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_009669730.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis neuronal guanine nucleotide exchange factor (NGEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009669730.1

ORF Insert Sequence:

ATGCAAAACA AATACCCATT CCCACAATGC ATTCCTACTT TTTTTGTTCA GTGTAACCTC 
TCAGATCTGT TATATTGCCT TCTAGGGCTC TTGTATCAGG AATATCGAGA TAAATCCACG 
CGCCAGGAGA TTGAAACCAG GCGGCTGCAG GATTCACAGA CAGACCCTGA AGAGAATTCA 
GCCAGCGAGG AAACTCCATC TGAAACAGAA GCTGCAAGTA CAACTGAAAG CAAAGTGGCA 
CCTCAAATCA GTTTGCTGAG GAACTCCACC TCCCGGTTCA ACTTATGGCA GGATCTTCCT 
GAGATCCGGA GCAGTGGGGT CCTTAGCATC CTCCAGCCAG ATGAGATCAA GCTTCAGGAG 
GCCATGTTTG AACTGGTTAC TTCTGAAGCC TCGTATTACA AGAGTCTGAA CCTGCTGGTG 
TCTCACTTCA TGGAAAACGA ACGCCTGAAA AAGATCTTAC ATCAGTCTGA GGCACACATC 
CTCTTCTCCA ATGTGCTGGA TGTCATGGCT GTTAGTGAGC GGTTCCTGTT GGACCTTGAG 
CAGCGGGTGG AGGAGAATAT TGTGATCTCA GACGTGTGTG ACATTGTCTA CCAGCATGCG 
GTTAATCACT TCTCCGTGTA CATCACCTAC GTCTCCAACC AGACCTACCA GGAGAGAGCT 
TACAAGCAGC TTCTCCAGGA CAAGCCAGCT TTCCGGGAAG TGATATCACA GCTGGAGCTG 
GATCCCAAGT GCAAAGGCCT GTCCTTTTCT TCCTTCCTGA TTTTGCCATT TCAAAGGATC 
ACTCGACTCA AGCTGCTGGT GCAGAACATT TTGAAGAAAG TGGAAGAGAA GTCCGAGAGG 
GAAACCACTG CCCTGGATGC ACATAAGGAG CTGGAAACAG TGGTGAAAGC ATGTAATGAA 
GGTGTCAGAA AGATGAGCCG AACAGAGCAG ATGATCAGCA TCCAGAAGAA ACTGGAATTT 
AAGATCAAGT CCGTGCCCAT CATCTCGCAC TCACGCTGGC TCCTGAAGCA GGGGGAACTG 
CAGCAAATGA ACGGTCCAAA GACATCACGT ACGCTTCGTA CCAAGAAACT CTTCAGAGAA 
ATCTACCTGT TCCTTTTCAA TGACCTGCTG GTAATTTGCA GGCAAATTCC TGGGGACAAG 
TACCAAGTGT TCGACTCAGC ACCCCGGGGA CTCCTTCGGG TGGAAGAGTT AGAAGACCAG 
GGGCAGAGTT TGGCCAACGT CTTCATCTTG AGGCTGCTGG AGAACGCAGA CGACCGGGAG 
GTCAGCTACA TGCTGAAGGC GTCGTCCCAG AGCGAGATGA AGCGCTGGAT GATTTCGTTA 
GCCCCGAACC GGAGAACCAA GTTCGTTTCG TTTACATCCA GACTCGTCGA CTGCCCGCAG 
ATCCAGTGCG TCCACCCGTA CGTGGCCCAG CAGCCGGACG AGCTGTCCTT GGAGCTGGCG 
GACGTCCTCA ACATCCTGGA CAAGACAGAT GACGGTTGGA TATTTGGGGA GCGTCTTCAC 
GACCAGGAGA GGGGCTGGTT CCCCAGTTCT ATGGGGGAGG AGATCCTGAA CCCCAAAATC 
CGAGCCCAGA ACTTGAAGGA GTGCTTCCGG GTGCACAAGT CGGATGACAG CCAGCGGAGG 
AAACTGGGCA GCAGAAATCG CCAATGA