LOC111227561 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111227561
Entrez Gene ID	111227561
Full Name	myosin light chain kinase 2, skeletal/cardiac muscle-like
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022753107.1	XP_022608828.1	myosin light chain kinase 2, skeletal/cardiac muscle-like

Pathways from BioSystems
Gene Ontology

The following LOC111227561 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111227561 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe200032	XM_022753107.1 Latest version!	Seriola dumerili myosin light chain kinase 2, skeletal/cardiac muscle-like (LOC111227561), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111227561 ( XM_022753107.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022608828.1 Seriola dumerili myosin light chain kinase 2, skeletal/cardiac muscle-like (LOC111227561), mRNA.

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CloneID	OSe200032
Clone ID Related Accession (Same CDS sequence)	XM_022753107.1
Accession Version	XM_022753107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	myosin light chain kinase 2, skeletal/cardiac muscle-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174444.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCA GCCTGGTCAA CTCTTTAGCC AAGGTTTATG ACCCAAATCC TCTTCACGGT 
CAGAAACCTG GTGGAAGGAA GCTGTCTCTT AATGGATCAG ACAGGTCCCA AGCTTCTTCA 
TCATCACCAT CCAGCTCCTC TGCTCATGAT GCCGCCCTGC GCTGCGTGGA GAGCCGAATG 
GACTCCCTGA GCTCTCAGAT GGAGCGCTTG CTCAACATGC AGCAGATTGT CTTGACTCGG 
CTGGACGGTC TGTCCCAGGA TGTCGGGGGG ATGGGTCGAG ATCTGGCCTC TATGTGTGAA 
GAGGGCCACG GGGGACGACG TGGGTCGGGG TTGGAGGCGG CGTGCAGGGA GCTGCGTGGG 
GCTGTGGAGA GGGCCAGCGA GCGGATGGAG AGTCAGAGAC GCAGGCTGGA GGGGGTTGAG 
AAGCTGGTGG AGGGCACGCA GCAGGTGATC AGCTTTATCG GGGAGGTGGT GAAGAGCTCC 
AGGCTGGTGG AGCTGCTGTT CAAACAGCCT GGAAACAAGG CCAACAAGAA GGCAAAGGAT 
GACAAGGACA AAGCCAAACT GAGCCTGAAG GGCTTGAAGG CCCAGAAGAA GAGGAAGCCC 
CCGGACACGT CGGATTCATC CTCCCTGACC GAGCCTGTGC TGCAGGAACA GGTGGAAAAG 
CTCAACAGGC AGAACACTGA GCATTCCCAC ATCAACGCAG AGGCTGAAGC CCAGAGGGGG 
GAGGACAGAG AAAGACAGGA GCAAGCAGAG CTGATCCTGG AAGGGTCGCA CACCTCCGCC 
ACGCAGTCAG AGGAGAGGAA GAAGACAACC AAGGAGAGAG AGGAGGGAGA GGAGGAGGGG 
GACGTCTTTC AGGACTCGGA GCCTGAGTTG GACGAGGAGA AGGAAGAAGA TGTCAGTGCA 
GAGTCGGCAG GAAAAGTGGA GGATGATGGG AAGAAAAGGC ATAAAGGAGA GGCAGGAGAG 
TCGACAAAAT CAGAAAAGTC CCAGAGCATC ATCGCAGACA TTTGCAAGAC CTCTCTCCTC 
CCACAGGAAA GCTCTGAGGA GGCCGGTGAC GTGGCCACCT GCAGCAAACG TCGTGTCACA 
GAAGAAGACC TGGTGAAGGA CGACCTCAAA AAAAGCAGAG TGGACAGTAA AGTGGGAAAC 
GCTGATGGAC TGGTGGTGGA GGGGGGCGAG ACGCAGGCCT TTAAATCTGA TGAAGCCAAG 
ACAGATGTGG AAGAGGAGGA GGGAGAGTCA GGGGTGAAAG AATTTATTAT TGACTCCAGC 
CCTCCACCGT TGGCACCTTT CGACCACCGC ATCGTGACTC CCAAACCCCA TCAGATCGCC 
ACCTATTACA CCATCAACAG AGATGAAGTC CTGGGAGGGG GACGTTTTGG TCAGGTCCAC 
AAGTGCATGG AGAACTCATC TGGTCTGACG TTGGCTGCCA AGATCATCAA AGCCAGGAGC 
CAGAAAGAGA AGGAGGTGGT GAGGAATGAG ATCCAGGTGA TGAACCAGCT GAACCACGCC 
AACCTCATCC AGCTGTACGC CGCCTTCGAG TCCCGCCACG ATATCATCCT AGTCATGGAA 
TATGTGGAGG GAGGGGAGCT GTTTGACCGC ATCATCGATG AGAACTACAA CCTGACAGAG 
CTGGACACGG TGCTGTTTAT ACGTCAGATC TGTGAAGGGC TGCAGTACAT GCATAAAATG 
TACATCCTGC ACCTCGACCT CAAGCCAGAG AACATCCTCT GTGTCAGCAG AGCTACTAAC 
AAGATTAAAA TCATTGACTT TGGCCTGGCC AGGAGGTATA AACCCAGAGA GAAGCTGAGG 
GTCAACTTTG GAACACCTGA ATTTTTAGCT CCTGAAGTCA TCAACTATGA GTTTGTTTCA 
TTCCCAACAG ACATGTGGAG TCTTGGTGTC ATCACTTACA TGCTGCTCAG CGGTTTGTCT 
CCGTTTCTGG GGGACGACGA CAATGAGACG CTGAACAACA TCCTGGCCTG TCAGTGGAAC 
TTTGAGGAGG AGGAGTTTAC GGACATCTCC GATGAGGCCA AAGACTTCAT CACCCGTCTG 
CTGGTGAAGA GCAAAAGCTG GAGGATGAGC GCTGCAGAGT CACTCAGACA CCCATGGCTC 
TCAGACCGGA GTCTGCACTA CCGCCTAAAT CAGAAGAAAA ACAAGTGCCA CTCCACACAT 
GCTCCTTCTC CAGAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022608828.1
CDS	199..2379
Translation	MSSSLVNSLAKVYDPNPLHGQKPGGRKLSLNGSDRSQASSSSPSSSSAHDAALRCVESRMDSLSSQMERLLNMQQIVLTRLDGLSQDVGGMGRDLASMCEEGHGGRRGSGLEAACRELRGAVERASERMESQRRRLEGVEKLVEGTQQVISFIGEVVKSSRLVELLFKQPGNKANKKAKDDKDKAKLSLKGLKAQKKRKPPDTSDSSSLTEPVLQEQVEKLNRQNTEHSHINAEAEAQRGEDRERQEQAELILEGSHTSATQSEERKKTTKEREEGEEEGDVFQDSEPELDEEKEEDVSAESAGKVEDDGKKRHKGEAGESTKSEKSQSIIADICKTSLLPQESSEEAGDVATCSKRRVTEEDLVKDDLKKSRVDSKVGNADGLVVEGGETQAFKSDEAKTDVEEEEGESGVKEFIIDSSPPPLAPFDHRIVTPKPHQIATYYTINRDEVLGGGRFGQVHKCMENSSGLTLAAKIIKARSQKEKEVVRNEIQVMNQLNHANLIQLYAAFESRHDIILVMEYVEGGELFDRIIDENYNLTELDTVLFIRQICEGLQYMHKMYILHLDLKPENILCVSRATNKIKIIDFGLARRYKPREKLRVNFGTPEFLAPEVINYEFVSFPTDMWSLGVITYMLLSGLSPFLGDDDNETLNNILACQWNFEEEEFTDISDEAKDFITRLLVKSKSWRMSAAESLRHPWLSDRSLHYRLNQKKNKCHSTHAPSPES

Target ORF information:

RefSeq Version	XM_022753107.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili myosin light chain kinase 2, skeletal/cardiac muscle-like (LOC111227561), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022753107.1

ORF Insert Sequence:

ATGAGCTCCA GCCTGGTCAA CTCTTTAGCC AAGGTTTATG ACCCAAATCC TCTTCACGGT 
CAGAAACCTG GTGGAAGGAA GCTGTCTCTT AATGGATCAG ACAGGTCCCA AGCTTCTTCA 
TCATCACCAT CCAGCTCCTC TGCTCATGAT GCCGCCCTGC GCTGCGTGGA GAGCCGAATG 
GACTCCCTGA GCTCTCAGAT GGAGCGCTTG CTCAACATGC AGCAGATTGT CTTGACTCGG 
CTGGACGGTC TGTCCCAGGA TGTCGGGGGG ATGGGTCGAG ATCTGGCCTC TATGTGTGAA 
GAGGGCCACG GGGGACGACG TGGGTCGGGG TTGGAGGCGG CGTGCAGGGA GCTGCGTGGG 
GCTGTGGAGA GGGCCAGCGA GCGGATGGAG AGTCAGAGAC GCAGGCTGGA GGGGGTTGAG 
AAGCTGGTGG AGGGCACGCA GCAGGTGATC AGCTTTATCG GGGAGGTGGT GAAGAGCTCC 
AGGCTGGTGG AGCTGCTGTT CAAACAGCCT GGAAACAAGG CCAACAAGAA GGCAAAGGAT 
GACAAGGACA AAGCCAAACT GAGCCTGAAG GGCTTGAAGG CCCAGAAGAA GAGGAAGCCC 
CCGGACACGT CGGATTCATC CTCCCTGACC GAGCCTGTGC TGCAGGAACA GGTGGAAAAG 
CTCAACAGGC AGAACACTGA GCATTCCCAC ATCAACGCAG AGGCTGAAGC CCAGAGGGGG 
GAGGACAGAG AAAGACAGGA GCAAGCAGAG CTGATCCTGG AAGGGTCGCA CACCTCCGCC 
ACGCAGTCAG AGGAGAGGAA GAAGACAACC AAGGAGAGAG AGGAGGGAGA GGAGGAGGGG 
GACGTCTTTC AGGACTCGGA GCCTGAGTTG GACGAGGAGA AGGAAGAAGA TGTCAGTGCA 
GAGTCGGCAG GAAAAGTGGA GGATGATGGG AAGAAAAGGC ATAAAGGAGA GGCAGGAGAG 
TCGACAAAAT CAGAAAAGTC CCAGAGCATC ATCGCAGACA TTTGCAAGAC CTCTCTCCTC 
CCACAGGAAA GCTCTGAGGA GGCCGGTGAC GTGGCCACCT GCAGCAAACG TCGTGTCACA 
GAAGAAGACC TGGTGAAGGA CGACCTCAAA AAAAGCAGAG TGGACAGTAA AGTGGGAAAC 
GCTGATGGAC TGGTGGTGGA GGGGGGCGAG ACGCAGGCCT TTAAATCTGA TGAAGCCAAG 
ACAGATGTGG AAGAGGAGGA GGGAGAGTCA GGGGTGAAAG AATTTATTAT TGACTCCAGC 
CCTCCACCGT TGGCACCTTT CGACCACCGC ATCGTGACTC CCAAACCCCA TCAGATCGCC 
ACCTATTACA CCATCAACAG AGATGAAGTC CTGGGAGGGG GACGTTTTGG TCAGGTCCAC 
AAGTGCATGG AGAACTCATC TGGTCTGACG TTGGCTGCCA AGATCATCAA AGCCAGGAGC 
CAGAAAGAGA AGGAGGTGGT GAGGAATGAG ATCCAGGTGA TGAACCAGCT GAACCACGCC 
AACCTCATCC AGCTGTACGC CGCCTTCGAG TCCCGCCACG ATATCATCCT AGTCATGGAA 
TATGTGGAGG GAGGGGAGCT GTTTGACCGC ATCATCGATG AGAACTACAA CCTGACAGAG 
CTGGACACGG TGCTGTTTAT ACGTCAGATC TGTGAAGGGC TGCAGTACAT GCATAAAATG 
TACATCCTGC ACCTCGACCT CAAGCCAGAG AACATCCTCT GTGTCAGCAG AGCTACTAAC 
AAGATTAAAA TCATTGACTT TGGCCTGGCC AGGAGGTATA AACCCAGAGA GAAGCTGAGG 
GTCAACTTTG GAACACCTGA ATTTTTAGCT CCTGAAGTCA TCAACTATGA GTTTGTTTCA 
TTCCCAACAG ACATGTGGAG TCTTGGTGTC ATCACTTACA TGCTGCTCAG CGGTTTGTCT 
CCGTTTCTGG GGGACGACGA CAATGAGACG CTGAACAACA TCCTGGCCTG TCAGTGGAAC 
TTTGAGGAGG AGGAGTTTAC GGACATCTCC GATGAGGCCA AAGACTTCAT CACCCGTCTG 
CTGGTGAAGA GCAAAAGCTG GAGGATGAGC GCTGCAGAGT CACTCAGACA CCCATGGCTC 
TCAGACCGGA GTCTGCACTA CCGCCTAAAT CAGAAGAAAA ACAAGTGCCA CTCCACACAT 
GCTCCTTCTC CAGAGAGCTA A