tie1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	tie1
Entrez Gene ID	111224483
Full Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022749021.1	XP_022604742.1	tyrosine-protein kinase receptor Tie-1 isoform X1
XM_022749104.1	XP_022604825.1	tyrosine-protein kinase receptor Tie-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following tie1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tie1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe206193	XM_022749021.1 Latest version!	Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSe206194	XM_022749104.1 Latest version!	Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tie1 ( XM_022749021.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022604742.1 Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe206193
Clone ID Related Accession (Same CDS sequence)	XM_022749021.1
Accession Version	XM_022749021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174257.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATTT TGTGGGTGTT TGTATTGTTC TTCATTGATT TATCAGATGC TGTGATCGAC 
TTGACCATGA TCTCCACAGC AGAGGTCAAT GGCATCAATC AGTTCACCAT CTCTTGTATC 
AATGGAGAGC GTAGCCCTGG CCAGGTTGAG CTGGACATCA AGAGAGACAA CAAAATCCTC 
ATATTCCCCA AGAAGCCAAA CTTTAAAGTG TCTAAGCCCA GGAACAATGA GGTGAAGGTC 
AAAGACTTCC GCGACCTGGA TAATATTGGT ATCTTCTACT GTGAATCCAT TCAGGAAGTT 
CCCCCTTTCG AGATGGTCAC GATGATCAAC AACTTCGGCG GAGCAAATTT TATTCCAACT 
CACCTCACTC TGACTGCTAA CAAAGGAGAG ACAGTTCACC TCAGCATGGA GCTACACAGC 
ACCCAAAAGA GAGATGTGAC CTGGAAATAC AACGGAAACT ACTATTATAT GACTCACTGG 
ATCGATATGA ACAATCACAC TGCTGTACTC ACAGTGGAGA ATGCAGCTTT TGCCAATCAA 
GGCATCTATA GTGCCAGCTA TGTGGGGGAC AGCCCGCTCC AGGGGGCCTG GATGAGGCTC 
ATTGTCCGAG ACTGTCCCAG TAACAAGTGG GGTCCTTACT GTAATCAGGA CTGTCCCGAG 
TGTCTAAATG GAGGAGTGTG TCACGATGCG GATGGAGACT GTGTCTGCCC TCCAGGATTC 
ATGGGAACTC GTTGTGAGAC AGCCTGCAGA GAAGGAATGT TTGGTCAAAA CTGCCAGGAG 
TCATGTGGCT CTGAGCTGAA CTGCAAGGGG CTTCGTTTCT GCCTACCAGA CCCTTATGGC 
TGTTCTTGTG CCAGCGGCTG GTTTGGCAGC CGCTGTGAGA AGGCCTGTGA TAAAGACATG 
TATGGACCAG ACTGCAGGCT GAGCTGTAAA TGCCAGAATG GAGGCGTTTG TAACCGTTTC 
AGTGGATGCC AGTGTCCCAC GGGGTGGAGA GGACCGAACT GCAAGAAGTC TGACCGGGCT 
CCCCAGATCT TGGACCTGGC TAGTAATTTG GAGTTGAATC TCAACTCCAG CCCCAAGATC 
CTTTGCTCAG CCACAGGAAA CCCCCTGCCC AACCACAATA GCATTGAGCT GCGAAAGTTG 
GACAGCACTG TGCTCAAGGC GTCCCAGACC GTCATAGAAT CAAATAAGAG TACAGCCCTG 
TTTGAGATCC ATCGTCTGAG TGCTCAGCAA AGTGGGTTGT GGGAGTGCAG GGTTTACACC 
AATGGAGGTC AGGACTCCCG CAAGTTCAAC CTCACCATCA AAGAGCCCCC CGTGCCCACT 
ACTGCCCCCA GACTGCTGGA AAAGAGGAGT AAGCAGCTGC TAGTGATGCC AGTGGACTCC 
CACCGAGGAG ACGGCCCCAT TGTCTCCACC AGGATCCTCT ACAAGCCTGT GGACAACAGA 
GGCTCTTGGT CTTCCATCAT AGTCTACAGT GACAAGGAGC CGATCACACT GATGAACTTG 
GAGCCTTCCA AACGCTACCG TGTCTGTGTC CAGCTGAGCC GTCCTGGGGA AGGAGGAGAG 
GGGGACCATG GGCCAGAGGC CATCATGGAG ACTGATTGTC CCGAGCCCAC AGTTCAACCT 
GAGATTGACA GCAGTTCGTT GGAAGGCCGC AATGCCACTG TACGCTGGCG ACTGCCCACC 
AACAATGGCA TTAATGCTGG CACAGCCACT GGCTTTTTAG TGCAGCTCTT CAGGCCACCG 
CCCTACAATG AGAAACTGCT GGAGGTGACC ACGCTGCTCA ATGTGCTTTC CACCAAGTTC 
CACAACCTGG AGTACCAGCA AGACTACATC GTGGTGGTAC AGCTCATCAA CTGTGGCAGC 
CAGGGGCCGC TGTCCAGTCC CTACCACTTC CGCATCAACA GCCAGGGCCC ATCGTCCCCA 
CGCAACGTCC AGGCCCTGCC CCTGTCTGTG TCAGCCATTC AGGTGAGGTG GCAGCCCCCC 
GAAGATCCCA ACGGAGGCAT CATTAAATAC ATCATCGAGT ACCAGCCAGT CGGCCAGGGG 
AGCCCTCACC CTTGGGTGGA TACGGACGAT GGGAACAAGA CCACCAAAGA TGTGACAGCG 
CTGAACGGCA GTACACTCTA CCAATTCAGA GTGAGGGCTT TCTCCAAAGT GCCGGGAGAG 
TGGAGCAAGG TTGTTCAGGC AATGACACAA GGAGATGGCC TGCAGAGTTC AACCCCGACC 
ACCCAGGGTG TGGCTGGGAG GTCCCCTGGG GACAGTTACC AGCTGCTGGT GGCAGTGGTG 
GGCTCGGTGA CCGTCACCTG TGTGACTATC CTGTTGGCAC TGCTGGCTCT TTTCTTCATC 
CGCAAGACGC TGCTCAACCG TCGACGCACA TTCACCTACC AGTCTGGATC AGGTGAGGAG 
ACTATTCTGC AGTTTAACTC AGGAACTCTG ACCCTGACAC GGAGGCCCAA GCCCACCCCA 
GAGCCCCTCA CCTATCCGAT CCTCGAGTGG GAGGACATTA AGTTTGAAGA TGTCATCGGA 
GAGGGCAACT TCGGCCAGGT GATCAAAGCC ATGATCAAGA AAGACGGCAA CAAGATGAGT 
GCAGCCATCA AGATGCTGAA AGAGTTTGCC TCAGAGAATG ACCACAGAGA CTTTGCAGGG 
GAGCTGGAGG TGCTGTGTAA ACTAGGCCAG CATCCCAACA TCATCAACCT CATTGGAGCC 
TGTGAGAACA GAGGTTACCT GTACATAGCC ATCGAATATG CTCCCTACGG TAACCTCCTC 
GACTTTCTGA GGAAGAGTCG AGTGCTGGAG ACCGACCCTG CCTTTGCAAA AGAGCACGGA 
ACTGCCTCCA CCCTCACCTC CCAACAGCTG CTGCAGTTTG CTGTTGACGT GTCCACTGGG 
ATGCATTACC TAAGTGACAA ACAGTTCATT CACAGGGATT TGGCAGCAAG GAATGTTCTT 
GTAGGGGACA ACCTCGTGGC AAAGATAGCA GACTTCGGCC TGTCCCGCGG TGAGGAAGTC 
TACGTTAAGA AGACAATGGG GAGGCTGCCT GTGCGCTGGA TGGCCATCGA ATCCCTGAAC 
TACAGCGTGT ATACGACCAA GAGTGATGTG TGGTCATTTG GTGTTCTCCT CTGGGAAATT 
GTGAGCTTAG GTGGCACGCC ATATTGTGGG ATGACATGTG CAGAACTTTA TGAAAAACTG 
CCACAAGGAT ACAGGATGGA GAAACCCAAA AACTGTGATA ATGAGGTCTA TGAGCTGATG 
AAGCAGTGCT GGAGGGATCG GACTTATGAG AGACCCCCCT TCTCCCAAAT CTCTGTCCAG 
CTCAACAGGA TGCAGGAGGC CAGGAAGGCT TATGTGAATA TGGCCCTCTT TGAGAACTTC 
ACCTACGCTG GAATAGACGC CACAGCTGAG GAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022604742.1
CDS	294..3692
Translation	MRILWVFVLFFIDLSDAVIDLTMISTAEVNGINQFTISCINGERSPGQVELDIKRDNKILIFPKKPNFKVSKPRNNEVKVKDFRDLDNIGIFYCESIQEVPPFEMVTMINNFGGANFIPTHLTLTANKGETVHLSMELHSTQKRDVTWKYNGNYYYMTHWIDMNNHTAVLTVENAAFANQGIYSASYVGDSPLQGAWMRLIVRDCPSNKWGPYCNQDCPECLNGGVCHDADGDCVCPPGFMGTRCETACREGMFGQNCQESCGSELNCKGLRFCLPDPYGCSCASGWFGSRCEKACDKDMYGPDCRLSCKCQNGGVCNRFSGCQCPTGWRGPNCKKSDRAPQILDLASNLELNLNSSPKILCSATGNPLPNHNSIELRKLDSTVLKASQTVIESNKSTALFEIHRLSAQQSGLWECRVYTNGGQDSRKFNLTIKEPPVPTTAPRLLEKRSKQLLVMPVDSHRGDGPIVSTRILYKPVDNRGSWSSIIVYSDKEPITLMNLEPSKRYRVCVQLSRPGEGGEGDHGPEAIMETDCPEPTVQPEIDSSSLEGRNATVRWRLPTNNGINAGTATGFLVQLFRPPPYNEKLLEVTTLLNVLSTKFHNLEYQQDYIVVVQLINCGSQGPLSSPYHFRINSQGPSSPRNVQALPLSVSAIQVRWQPPEDPNGGIIKYIIEYQPVGQGSPHPWVDTDDGNKTTKDVTALNGSTLYQFRVRAFSKVPGEWSKVVQAMTQGDGLQSSTPTTQGVAGRSPGDSYQLLVAVVGSVTVTCVTILLALLALFFIRKTLLNRRRTFTYQSGSGEETILQFNSGTLTLTRRPKPTPEPLTYPILEWEDIKFEDVIGEGNFGQVIKAMIKKDGNKMSAAIKMLKEFASENDHRDFAGELEVLCKLGQHPNIINLIGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFAVDVSTGMHYLSDKQFIHRDLAARNVLVGDNLVAKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPKNCDNEVYELMKQCWRDRTYERPPFSQISVQLNRMQEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_022749021.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022749021.1

ORF Insert Sequence:

ATGAGGATTT TGTGGGTGTT TGTATTGTTC TTCATTGATT TATCAGATGC TGTGATCGAC 
TTGACCATGA TCTCCACAGC AGAGGTCAAT GGCATCAATC AGTTCACCAT CTCTTGTATC 
AATGGAGAGC GTAGCCCTGG CCAGGTTGAG CTGGACATCA AGAGAGACAA CAAAATCCTC 
ATATTCCCCA AGAAGCCAAA CTTTAAAGTG TCTAAGCCCA GGAACAATGA GGTGAAGGTC 
AAAGACTTCC GCGACCTGGA TAATATTGGT ATCTTCTACT GTGAATCCAT TCAGGAAGTT 
CCCCCTTTCG AGATGGTCAC GATGATCAAC AACTTCGGCG GAGCAAATTT TATTCCAACT 
CACCTCACTC TGACTGCTAA CAAAGGAGAG ACAGTTCACC TCAGCATGGA GCTACACAGC 
ACCCAAAAGA GAGATGTGAC CTGGAAATAC AACGGAAACT ACTATTATAT GACTCACTGG 
ATCGATATGA ACAATCACAC TGCTGTACTC ACAGTGGAGA ATGCAGCTTT TGCCAATCAA 
GGCATCTATA GTGCCAGCTA TGTGGGGGAC AGCCCGCTCC AGGGGGCCTG GATGAGGCTC 
ATTGTCCGAG ACTGTCCCAG TAACAAGTGG GGTCCTTACT GTAATCAGGA CTGTCCCGAG 
TGTCTAAATG GAGGAGTGTG TCACGATGCG GATGGAGACT GTGTCTGCCC TCCAGGATTC 
ATGGGAACTC GTTGTGAGAC AGCCTGCAGA GAAGGAATGT TTGGTCAAAA CTGCCAGGAG 
TCATGTGGCT CTGAGCTGAA CTGCAAGGGG CTTCGTTTCT GCCTACCAGA CCCTTATGGC 
TGTTCTTGTG CCAGCGGCTG GTTTGGCAGC CGCTGTGAGA AGGCCTGTGA TAAAGACATG 
TATGGACCAG ACTGCAGGCT GAGCTGTAAA TGCCAGAATG GAGGCGTTTG TAACCGTTTC 
AGTGGATGCC AGTGTCCCAC GGGGTGGAGA GGACCGAACT GCAAGAAGTC TGACCGGGCT 
CCCCAGATCT TGGACCTGGC TAGTAATTTG GAGTTGAATC TCAACTCCAG CCCCAAGATC 
CTTTGCTCAG CCACAGGAAA CCCCCTGCCC AACCACAATA GCATTGAGCT GCGAAAGTTG 
GACAGCACTG TGCTCAAGGC GTCCCAGACC GTCATAGAAT CAAATAAGAG TACAGCCCTG 
TTTGAGATCC ATCGTCTGAG TGCTCAGCAA AGTGGGTTGT GGGAGTGCAG GGTTTACACC 
AATGGAGGTC AGGACTCCCG CAAGTTCAAC CTCACCATCA AAGAGCCCCC CGTGCCCACT 
ACTGCCCCCA GACTGCTGGA AAAGAGGAGT AAGCAGCTGC TAGTGATGCC AGTGGACTCC 
CACCGAGGAG ACGGCCCCAT TGTCTCCACC AGGATCCTCT ACAAGCCTGT GGACAACAGA 
GGCTCTTGGT CTTCCATCAT AGTCTACAGT GACAAGGAGC CGATCACACT GATGAACTTG 
GAGCCTTCCA AACGCTACCG TGTCTGTGTC CAGCTGAGCC GTCCTGGGGA AGGAGGAGAG 
GGGGACCATG GGCCAGAGGC CATCATGGAG ACTGATTGTC CCGAGCCCAC AGTTCAACCT 
GAGATTGACA GCAGTTCGTT GGAAGGCCGC AATGCCACTG TACGCTGGCG ACTGCCCACC 
AACAATGGCA TTAATGCTGG CACAGCCACT GGCTTTTTAG TGCAGCTCTT CAGGCCACCG 
CCCTACAATG AGAAACTGCT GGAGGTGACC ACGCTGCTCA ATGTGCTTTC CACCAAGTTC 
CACAACCTGG AGTACCAGCA AGACTACATC GTGGTGGTAC AGCTCATCAA CTGTGGCAGC 
CAGGGGCCGC TGTCCAGTCC CTACCACTTC CGCATCAACA GCCAGGGCCC ATCGTCCCCA 
CGCAACGTCC AGGCCCTGCC CCTGTCTGTG TCAGCCATTC AGGTGAGGTG GCAGCCCCCC 
GAAGATCCCA ACGGAGGCAT CATTAAATAC ATCATCGAGT ACCAGCCAGT CGGCCAGGGG 
AGCCCTCACC CTTGGGTGGA TACGGACGAT GGGAACAAGA CCACCAAAGA TGTGACAGCG 
CTGAACGGCA GTACACTCTA CCAATTCAGA GTGAGGGCTT TCTCCAAAGT GCCGGGAGAG 
TGGAGCAAGG TTGTTCAGGC AATGACACAA GGAGATGGCC TGCAGAGTTC AACCCCGACC 
ACCCAGGGTG TGGCTGGGAG GTCCCCTGGG GACAGTTACC AGCTGCTGGT GGCAGTGGTG 
GGCTCGGTGA CCGTCACCTG TGTGACTATC CTGTTGGCAC TGCTGGCTCT TTTCTTCATC 
CGCAAGACGC TGCTCAACCG TCGACGCACA TTCACCTACC AGTCTGGATC AGGTGAGGAG 
ACTATTCTGC AGTTTAACTC AGGAACTCTG ACCCTGACAC GGAGGCCCAA GCCCACCCCA 
GAGCCCCTCA CCTATCCGAT CCTCGAGTGG GAGGACATTA AGTTTGAAGA TGTCATCGGA 
GAGGGCAACT TCGGCCAGGT GATCAAAGCC ATGATCAAGA AAGACGGCAA CAAGATGAGT 
GCAGCCATCA AGATGCTGAA AGAGTTTGCC TCAGAGAATG ACCACAGAGA CTTTGCAGGG 
GAGCTGGAGG TGCTGTGTAA ACTAGGCCAG CATCCCAACA TCATCAACCT CATTGGAGCC 
TGTGAGAACA GAGGTTACCT GTACATAGCC ATCGAATATG CTCCCTACGG TAACCTCCTC 
GACTTTCTGA GGAAGAGTCG AGTGCTGGAG ACCGACCCTG CCTTTGCAAA AGAGCACGGA 
ACTGCCTCCA CCCTCACCTC CCAACAGCTG CTGCAGTTTG CTGTTGACGT GTCCACTGGG 
ATGCATTACC TAAGTGACAA ACAGTTCATT CACAGGGATT TGGCAGCAAG GAATGTTCTT 
GTAGGGGACA ACCTCGTGGC AAAGATAGCA GACTTCGGCC TGTCCCGCGG TGAGGAAGTC 
TACGTTAAGA AGACAATGGG GAGGCTGCCT GTGCGCTGGA TGGCCATCGA ATCCCTGAAC 
TACAGCGTGT ATACGACCAA GAGTGATGTG TGGTCATTTG GTGTTCTCCT CTGGGAAATT 
GTGAGCTTAG GTGGCACGCC ATATTGTGGG ATGACATGTG CAGAACTTTA TGAAAAACTG 
CCACAAGGAT ACAGGATGGA GAAACCCAAA AACTGTGATA ATGAGGTCTA TGAGCTGATG 
AAGCAGTGCT GGAGGGATCG GACTTATGAG AGACCCCCCT TCTCCCAAAT CTCTGTCCAG 
CTCAACAGGA TGCAGGAGGC CAGGAAGGCT TATGTGAATA TGGCCCTCTT TGAGAACTTC 
ACCTACGCTG GAATAGACGC CACAGCTGAG GAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tie1 ( XM_022749104.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022604825.1 Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe206194
Clone ID Related Accession (Same CDS sequence)	XM_022749104.1
Accession Version	XM_022749104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174257.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATTT TGTGGGTGTT TGTATTGTTC TTCATTGATT TATCAGATGC TGTGATCGAC 
TTGACCATGA TCTCCACAGC AGAGGTCAAT GGCATCAATC AGTTCACCAT CTCTTGTATC 
AATGGAGAGC GTAGCCCTGG CCAGGTTGAG CTGGACATCA AGAGAGACAA CAAAATCCTC 
ATATTCCCCA AGAAGCCAAA CTTTAAAGTG TCTAAGCCCA GGAACAATGA GGTGAAGGTC 
AAAGACTTCC GCGACCTGGA TAATATTGGT ATCTTCTACT GTGAATCCAT TCAGGAAGTT 
CCCCCTTTCG AGATGGTCAC GATGATCAAC AACTTCGGCG GAGCAAATTT TATTCCAACT 
CACCTCACTC TGACTGCTAA CAAAGGAGAG ACAGTTCACC TCAGCATGGA GCTACACAGC 
ACCCAAAAGA GAGATGTGAC CTGGAAATAC AACGGAAACT ACTATTATAT GACTCACTGG 
ATCGATATGA ACAATCACAC TGCTGTACTC ACAGTGGAGA ATGCAGCTTT TGCCAATCAA 
GGCATCTATA GTGCCAGCTA TGTGGGGGAC AGCCCGCTCC AGGGGGCCTG GATGAGGCTC 
ATTGTCCGAG ACTGTCCCAG TAACAAGTGG GGTCCTTACT GTAATCAGGA CTGTCCCGAG 
TGTCTAAATG GAGGAGTGTG TCACGATGCG GATGGAGACT GTGTCTGCCC TCCAGGATTC 
ATGGGAACTC GTTGTGAGAC AGCCTGCAGA GAAGGAATGT TTGGTCAAAA CTGCCAGGAG 
TCATGTGGCT CTGAGCTGAA CTGCAAGGGG CTTCGTTTCT GCCTACCAGA CCCTTATGGC 
TGTTCTTGTG CCAGCGGCTG GTTTGGCAGC CGCTGTGAGA AGGCCTGTGA TAAAGACATG 
TATGGACCAG ACTGCAGGCT GAGCTGTAAA TGCCAGAATG GAGGCGTTTG TAACCGTTTC 
AGTGGATGCC AGTGTCCCAC GGGGTGGAGA GGACCGAACT GCAAGAAGTC TGACCGGGCT 
CCCCAGATCT TGGACCTGGC TAGTAATTTG GAGTTGAATC TCAACTCCAG CCCCAAGATC 
CTTTGCTCAG CCACAGGAAA CCCCCTGCCC AACCACAATA GCATTGAGCT GCGAAAGTTG 
GACAGCACTG TGCTCAAGGC GTCCCAGACC GTCATAGAAT CAAATAAGAG TACAGCCCTG 
TTTGAGATCC ATCGTCTGAG TGCTCAGCAA AGTGGGTTGT GGGAGTGCAG GGTTTACACC 
AATGGAGGTC AGGACTCCCG CAAGTTCAAC CTCACCATCA AAGAGCCCCC CGTGCCCACT 
ACTGCCCCCA GACTGCTGGA AAAGAGGAGT AAGCAGCTGC TAGTGATGCC AGTGGACTCC 
CACCGAGGAG ACGGCCCCAT TGTCTCCACC AGGATCCTCT ACAAGCCTGT GGACAACAGA 
GGCTCTTGGT CTTCCATCAT AGTCTACAGT GACAAGGAGC CGATCACACT GATGAACTTG 
GAGCCTTCCA AACGCTACCG TGTCTGTGTC CAGCTGAGCC GTCCTGGGGA AGGAGGAGAG 
GGGGACCATG GGCCAGAGGC CATCATGGAG ACTGATTGTC CCGGCCCATC GTCCCCACGC 
AACGTCCAGG CCCTGCCCCT GTCTGTGTCA GCCATTCAGG TGAGGTGGCA GCCCCCCGAA 
GATCCCAACG GAGGCATCAT TAAATACATC ATCGAGTACC AGCCAGTCGG CCAGGGGAGC 
CCTCACCCTT GGGTGGATAC GGACGATGGG AACAAGACCA CCAAAGATGT GACAGCGCTG 
AACGGCAGTA CACTCTACCA ATTCAGAGTG AGGGCTTTCT CCAAAGTGCC GGGAGAGTGG 
AGCAAGGTTG TTCAGGCAAT GACACAAGGA GATGGCCTGC AGAGTTCAAC CCCGACCACC 
CAGGGTGTGG CTGGGAGGTC CCCTGGGGAC AGTTACCAGC TGCTGGTGGC AGTGGTGGGC 
TCGGTGACCG TCACCTGTGT GACTATCCTG TTGGCACTGC TGGCTCTTTT CTTCATCCGC 
AAGACGCTGC TCAACCGTCG ACGCACATTC ACCTACCAGT CTGGATCAGG TGAGGAGACT 
ATTCTGCAGT TTAACTCAGG AACTCTGACC CTGACACGGA GGCCCAAGCC CACCCCAGAG 
CCCCTCACCT ATCCGATCCT CGAGTGGGAG GACATTAAGT TTGAAGATGT CATCGGAGAG 
GGCAACTTCG GCCAGGTGAT CAAAGCCATG ATCAAGAAAG ACGGCAACAA GATGAGTGCA 
GCCATCAAGA TGCTGAAAGA GTTTGCCTCA GAGAATGACC ACAGAGACTT TGCAGGGGAG 
CTGGAGGTGC TGTGTAAACT AGGCCAGCAT CCCAACATCA TCAACCTCAT TGGAGCCTGT 
GAGAACAGAG GTTACCTGTA CATAGCCATC GAATATGCTC CCTACGGTAA CCTCCTCGAC 
TTTCTGAGGA AGAGTCGAGT GCTGGAGACC GACCCTGCCT TTGCAAAAGA GCACGGAACT 
GCCTCCACCC TCACCTCCCA ACAGCTGCTG CAGTTTGCTG TTGACGTGTC CACTGGGATG 
CATTACCTAA GTGACAAACA GTTCATTCAC AGGGATTTGG CAGCAAGGAA TGTTCTTGTA 
GGGGACAACC TCGTGGCAAA GATAGCAGAC TTCGGCCTGT CCCGCGGTGA GGAAGTCTAC 
GTTAAGAAGA CAATGGGGAG GCTGCCTGTG CGCTGGATGG CCATCGAATC CCTGAACTAC 
AGCGTGTATA CGACCAAGAG TGATGTGTGG TCATTTGGTG TTCTCCTCTG GGAAATTGTG 
AGCTTAGGTG GCACGCCATA TTGTGGGATG ACATGTGCAG AACTTTATGA AAAACTGCCA 
CAAGGATACA GGATGGAGAA ACCCAAAAAC TGTGATAATG AGGTCTATGA GCTGATGAAG 
CAGTGCTGGA GGGATCGGAC TTATGAGAGA CCCCCCTTCT CCCAAATCTC TGTCCAGCTC 
AACAGGATGC AGGAGGCCAG GAAGGCTTAT GTGAATATGG CCCTCTTTGA GAACTTCACC 
TACGCTGGAA TAGACGCCAC AGCTGAGGAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022604825.1
CDS	293..3388
Translation	MRILWVFVLFFIDLSDAVIDLTMISTAEVNGINQFTISCINGERSPGQVELDIKRDNKILIFPKKPNFKVSKPRNNEVKVKDFRDLDNIGIFYCESIQEVPPFEMVTMINNFGGANFIPTHLTLTANKGETVHLSMELHSTQKRDVTWKYNGNYYYMTHWIDMNNHTAVLTVENAAFANQGIYSASYVGDSPLQGAWMRLIVRDCPSNKWGPYCNQDCPECLNGGVCHDADGDCVCPPGFMGTRCETACREGMFGQNCQESCGSELNCKGLRFCLPDPYGCSCASGWFGSRCEKACDKDMYGPDCRLSCKCQNGGVCNRFSGCQCPTGWRGPNCKKSDRAPQILDLASNLELNLNSSPKILCSATGNPLPNHNSIELRKLDSTVLKASQTVIESNKSTALFEIHRLSAQQSGLWECRVYTNGGQDSRKFNLTIKEPPVPTTAPRLLEKRSKQLLVMPVDSHRGDGPIVSTRILYKPVDNRGSWSSIIVYSDKEPITLMNLEPSKRYRVCVQLSRPGEGGEGDHGPEAIMETDCPGPSSPRNVQALPLSVSAIQVRWQPPEDPNGGIIKYIIEYQPVGQGSPHPWVDTDDGNKTTKDVTALNGSTLYQFRVRAFSKVPGEWSKVVQAMTQGDGLQSSTPTTQGVAGRSPGDSYQLLVAVVGSVTVTCVTILLALLALFFIRKTLLNRRRTFTYQSGSGEETILQFNSGTLTLTRRPKPTPEPLTYPILEWEDIKFEDVIGEGNFGQVIKAMIKKDGNKMSAAIKMLKEFASENDHRDFAGELEVLCKLGQHPNIINLIGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFAVDVSTGMHYLSDKQFIHRDLAARNVLVGDNLVAKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPKNCDNEVYELMKQCWRDRTYERPPFSQISVQLNRMQEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_022749104.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili tyrosine kinase with immunoglobulin like and EGF like domains 1 (tie1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022749104.1

ORF Insert Sequence:

ATGAGGATTT TGTGGGTGTT TGTATTGTTC TTCATTGATT TATCAGATGC TGTGATCGAC 
TTGACCATGA TCTCCACAGC AGAGGTCAAT GGCATCAATC AGTTCACCAT CTCTTGTATC 
AATGGAGAGC GTAGCCCTGG CCAGGTTGAG CTGGACATCA AGAGAGACAA CAAAATCCTC 
ATATTCCCCA AGAAGCCAAA CTTTAAAGTG TCTAAGCCCA GGAACAATGA GGTGAAGGTC 
AAAGACTTCC GCGACCTGGA TAATATTGGT ATCTTCTACT GTGAATCCAT TCAGGAAGTT 
CCCCCTTTCG AGATGGTCAC GATGATCAAC AACTTCGGCG GAGCAAATTT TATTCCAACT 
CACCTCACTC TGACTGCTAA CAAAGGAGAG ACAGTTCACC TCAGCATGGA GCTACACAGC 
ACCCAAAAGA GAGATGTGAC CTGGAAATAC AACGGAAACT ACTATTATAT GACTCACTGG 
ATCGATATGA ACAATCACAC TGCTGTACTC ACAGTGGAGA ATGCAGCTTT TGCCAATCAA 
GGCATCTATA GTGCCAGCTA TGTGGGGGAC AGCCCGCTCC AGGGGGCCTG GATGAGGCTC 
ATTGTCCGAG ACTGTCCCAG TAACAAGTGG GGTCCTTACT GTAATCAGGA CTGTCCCGAG 
TGTCTAAATG GAGGAGTGTG TCACGATGCG GATGGAGACT GTGTCTGCCC TCCAGGATTC 
ATGGGAACTC GTTGTGAGAC AGCCTGCAGA GAAGGAATGT TTGGTCAAAA CTGCCAGGAG 
TCATGTGGCT CTGAGCTGAA CTGCAAGGGG CTTCGTTTCT GCCTACCAGA CCCTTATGGC 
TGTTCTTGTG CCAGCGGCTG GTTTGGCAGC CGCTGTGAGA AGGCCTGTGA TAAAGACATG 
TATGGACCAG ACTGCAGGCT GAGCTGTAAA TGCCAGAATG GAGGCGTTTG TAACCGTTTC 
AGTGGATGCC AGTGTCCCAC GGGGTGGAGA GGACCGAACT GCAAGAAGTC TGACCGGGCT 
CCCCAGATCT TGGACCTGGC TAGTAATTTG GAGTTGAATC TCAACTCCAG CCCCAAGATC 
CTTTGCTCAG CCACAGGAAA CCCCCTGCCC AACCACAATA GCATTGAGCT GCGAAAGTTG 
GACAGCACTG TGCTCAAGGC GTCCCAGACC GTCATAGAAT CAAATAAGAG TACAGCCCTG 
TTTGAGATCC ATCGTCTGAG TGCTCAGCAA AGTGGGTTGT GGGAGTGCAG GGTTTACACC 
AATGGAGGTC AGGACTCCCG CAAGTTCAAC CTCACCATCA AAGAGCCCCC CGTGCCCACT 
ACTGCCCCCA GACTGCTGGA AAAGAGGAGT AAGCAGCTGC TAGTGATGCC AGTGGACTCC 
CACCGAGGAG ACGGCCCCAT TGTCTCCACC AGGATCCTCT ACAAGCCTGT GGACAACAGA 
GGCTCTTGGT CTTCCATCAT AGTCTACAGT GACAAGGAGC CGATCACACT GATGAACTTG 
GAGCCTTCCA AACGCTACCG TGTCTGTGTC CAGCTGAGCC GTCCTGGGGA AGGAGGAGAG 
GGGGACCATG GGCCAGAGGC CATCATGGAG ACTGATTGTC CCGGCCCATC GTCCCCACGC 
AACGTCCAGG CCCTGCCCCT GTCTGTGTCA GCCATTCAGG TGAGGTGGCA GCCCCCCGAA 
GATCCCAACG GAGGCATCAT TAAATACATC ATCGAGTACC AGCCAGTCGG CCAGGGGAGC 
CCTCACCCTT GGGTGGATAC GGACGATGGG AACAAGACCA CCAAAGATGT GACAGCGCTG 
AACGGCAGTA CACTCTACCA ATTCAGAGTG AGGGCTTTCT CCAAAGTGCC GGGAGAGTGG 
AGCAAGGTTG TTCAGGCAAT GACACAAGGA GATGGCCTGC AGAGTTCAAC CCCGACCACC 
CAGGGTGTGG CTGGGAGGTC CCCTGGGGAC AGTTACCAGC TGCTGGTGGC AGTGGTGGGC 
TCGGTGACCG TCACCTGTGT GACTATCCTG TTGGCACTGC TGGCTCTTTT CTTCATCCGC 
AAGACGCTGC TCAACCGTCG ACGCACATTC ACCTACCAGT CTGGATCAGG TGAGGAGACT 
ATTCTGCAGT TTAACTCAGG AACTCTGACC CTGACACGGA GGCCCAAGCC CACCCCAGAG 
CCCCTCACCT ATCCGATCCT CGAGTGGGAG GACATTAAGT TTGAAGATGT CATCGGAGAG 
GGCAACTTCG GCCAGGTGAT CAAAGCCATG ATCAAGAAAG ACGGCAACAA GATGAGTGCA 
GCCATCAAGA TGCTGAAAGA GTTTGCCTCA GAGAATGACC ACAGAGACTT TGCAGGGGAG 
CTGGAGGTGC TGTGTAAACT AGGCCAGCAT CCCAACATCA TCAACCTCAT TGGAGCCTGT 
GAGAACAGAG GTTACCTGTA CATAGCCATC GAATATGCTC CCTACGGTAA CCTCCTCGAC 
TTTCTGAGGA AGAGTCGAGT GCTGGAGACC GACCCTGCCT TTGCAAAAGA GCACGGAACT 
GCCTCCACCC TCACCTCCCA ACAGCTGCTG CAGTTTGCTG TTGACGTGTC CACTGGGATG 
CATTACCTAA GTGACAAACA GTTCATTCAC AGGGATTTGG CAGCAAGGAA TGTTCTTGTA 
GGGGACAACC TCGTGGCAAA GATAGCAGAC TTCGGCCTGT CCCGCGGTGA GGAAGTCTAC 
GTTAAGAAGA CAATGGGGAG GCTGCCTGTG CGCTGGATGG CCATCGAATC CCTGAACTAC 
AGCGTGTATA CGACCAAGAG TGATGTGTGG TCATTTGGTG TTCTCCTCTG GGAAATTGTG 
AGCTTAGGTG GCACGCCATA TTGTGGGATG ACATGTGCAG AACTTTATGA AAAACTGCCA 
CAAGGATACA GGATGGAGAA ACCCAAAAAC TGTGATAATG AGGTCTATGA GCTGATGAAG 
CAGTGCTGGA GGGATCGGAC TTATGAGAGA CCCCCCTTCT CCCAAATCTC TGTCCAGCTC 
AACAGGATGC AGGAGGCCAG GAAGGCTTAT GTGAATATGG CCCTCTTTGA GAACTTCACC 
TACGCTGGAA TAGACGCCAC AGCTGAGGAG GCCTGA