lcmt1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	lcmt1
Entrez Gene ID	111220064
Full Name	leucine carboxyl methyltransferase 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022743010.1	XP_022598731.1	leucine carboxyl methyltransferase 1

Pathways from BioSystems
Gene Ontology

The following lcmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lcmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe213537	XM_022743010.1 Latest version!	Seriola dumerili leucine carboxyl methyltransferase 1 (lcmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lcmt1 ( XM_022743010.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022598731.1 Seriola dumerili leucine carboxyl methyltransferase 1 (lcmt1), mRNA.

Price & Availability↑

CloneID	OSe213537
Clone ID Related Accession (Same CDS sequence)	XM_022743010.1
Accession Version	XM_022743010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	leucine carboxyl methyltransferase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174323.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTAT GCAGCGTTTA CAGTTTTAAT GTTGACCAAA TAACGTTAGG AGAAGGATCA 
TTAATGGCAG CACGGCAACC CTTCACAGAC ACGGATACTG CAGATGAAGC AGTGAGGACG 
ACATGTGACG ATGCAACCAC ATGTAAAAGG TTTGCCACCA GTAAAGGCTA CTGGAAGGAC 
CCATATATCC AGTACTTTGT GAGATCTGTA GGCGAAAGGA AGGCACCTGA AATCAACAGG 
GGCTACTATG CCCGTGTTCA AGGAGTGAAC CATCTACTTG ATGCGTTTAT AAAGAAAGCA 
GAATGTGACT GTCAAATCAT TAACCTGGGA GCTGGGCTGG ACACCACGTT TTGGAGACTG 
AAGGATGAAA ATCTCATGCC ACGGAAGTTC TTTGAAGTTG ACTTTCCTAC CATTGTGGCC 
AGGAAAATAC ACAATATAAA GACAAAACCA CCGCTGTCCA AACCCATCAT CGAAACCCAC 
TCAACAGACT CCTTACTGCT AGATGCCCAC AGCCTCGACT CAGACCGTTA CTGTATCATT 
GGAGCAGACC TCAGAGACAT CTCTAGTTTG GATGAGAAAC TGAAGAAGTT CCAGCTTAAC 
CCAGAATTAC CCACACTGCT CCTGTCCGAG TGCGTGCTGG TCTACATGAC GCCCTTCCAG 
TCCTCCAACC TAGTTAACTG GGCAGCGGAG ACCTTCCACA CCGCCATGTT CATCAACTAC 
GAACAGGTGA ACATGAGCGA TCGATTTGGC CAGGTGATGA TCGAGAACCT GCAGCGCCGA 
CAGTGCACCC TGGCAGGAGT CGAGCTCTGC CAATCCCTGG ACTCTCAGAA GGAGCGGTTC 
CTGAAGGCCG GCTGGGAGCA TGCCGATGCG CTGGACATGA TGACGATCTA CAGCATGCTC 
CGTCAGGACG ACGTGGCAAG AATTGAGCGA CTGGAGTTCC TGGACGAGAA GGAGCTGCTG 
CAGCAGCTTC TTCAGCACTA CAGCATCAGC TGGGCCGCCA AGGACAAACT CAACCTGGGT 
CTGTCGCAGT TGGCATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022598731.1
CDS	252..1292
Translation	MRLCSVYSFNVDQITLGEGSLMAARQPFTDTDTADEAVRTTCDDATTCKRFATSKGYWKDPYIQYFVRSVGERKAPEINRGYYARVQGVNHLLDAFIKKAECDCQIINLGAGLDTTFWRLKDENLMPRKFFEVDFPTIVARKIHNIKTKPPLSKPIIETHSTDSLLLDAHSLDSDRYCIIGADLRDISSLDEKLKKFQLNPELPTLLLSECVLVYMTPFQSSNLVNWAAETFHTAMFINYEQVNMSDRFGQVMIENLQRRQCTLAGVELCQSLDSQKERFLKAGWEHADALDMMTIYSMLRQDDVARIERLEFLDEKELLQQLLQHYSISWAAKDKLNLGLSQLAF

Target ORF information:

RefSeq Version	XM_022743010.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili leucine carboxyl methyltransferase 1 (lcmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022743010.1

ORF Insert Sequence:

ATGAGATTAT GCAGCGTTTA CAGTTTTAAT GTTGACCAAA TAACGTTAGG AGAAGGATCA 
TTAATGGCAG CACGGCAACC CTTCACAGAC ACGGATACTG CAGATGAAGC AGTGAGGACG 
ACATGTGACG ATGCAACCAC ATGTAAAAGG TTTGCCACCA GTAAAGGCTA CTGGAAGGAC 
CCATATATCC AGTACTTTGT GAGATCTGTA GGCGAAAGGA AGGCACCTGA AATCAACAGG 
GGCTACTATG CCCGTGTTCA AGGAGTGAAC CATCTACTTG ATGCGTTTAT AAAGAAAGCA 
GAATGTGACT GTCAAATCAT TAACCTGGGA GCTGGGCTGG ACACCACGTT TTGGAGACTG 
AAGGATGAAA ATCTCATGCC ACGGAAGTTC TTTGAAGTTG ACTTTCCTAC CATTGTGGCC 
AGGAAAATAC ACAATATAAA GACAAAACCA CCGCTGTCCA AACCCATCAT CGAAACCCAC 
TCAACAGACT CCTTACTGCT AGATGCCCAC AGCCTCGACT CAGACCGTTA CTGTATCATT 
GGAGCAGACC TCAGAGACAT CTCTAGTTTG GATGAGAAAC TGAAGAAGTT CCAGCTTAAC 
CCAGAATTAC CCACACTGCT CCTGTCCGAG TGCGTGCTGG TCTACATGAC GCCCTTCCAG 
TCCTCCAACC TAGTTAACTG GGCAGCGGAG ACCTTCCACA CCGCCATGTT CATCAACTAC 
GAACAGGTGA ACATGAGCGA TCGATTTGGC CAGGTGATGA TCGAGAACCT GCAGCGCCGA 
CAGTGCACCC TGGCAGGAGT CGAGCTCTGC CAATCCCTGG ACTCTCAGAA GGAGCGGTTC 
CTGAAGGCCG GCTGGGAGCA TGCCGATGCG CTGGACATGA TGACGATCTA CAGCATGCTC 
CGTCAGGACG ACGTGGCAAG AATTGAGCGA CTGGAGTTCC TGGACGAGAA GGAGCTGCTG 
CAGCAGCTTC TTCAGCACTA CAGCATCAGC TGGGCCGCCA AGGACAAACT CAACCTGGGT 
CTGTCGCAGT TGGCATTTTG A