git1 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	git1
Entrez Gene ID	108932065
Full Name	GIT ArfGAP 1
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018748237.1	XP_018603753.1	ARF GTPase-activating protein GIT1 isoform X3
XM_018748235.2	XP_018603751.1	ARF GTPase-activating protein GIT1 isoform X1
XM_018748236.2	XP_018603752.1	ARF GTPase-activating protein GIT1 isoform X2
XM_018748237.2	XP_018603753.1	ARF GTPase-activating protein GIT1 isoform X3
XM_018748235.1	XP_018603751.1	ARF GTPase-activating protein GIT1 isoform X1
XM_018748236.1	XP_018603752.1	ARF GTPase-activating protein GIT1 isoform X2

Pathways from BioSystems
Gene Ontology

The following git1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the git1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc144053	XM_018748235.2 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc144054	XM_018748236.2 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc144055	XM_018748237.2 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc51745	XM_018748235.1 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSc51746	XM_018748236.1 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSc51747	XM_018748237.1 Latest version!	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

git1 ( XM_018748235.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603751.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc144053
Clone ID Related Accession (Same CDS sequence)	XM_018748235.2
Accession Version	XM_018748235.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018748235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GATCTCACGG ATGCAGATGG AGAACAGTGC GATGCGGGGG 
GGCCAGGAAG GGGGAACTGA CGTTTTGGGA GTGGGCAGTG GGTCAGGGCT TTGGGCTGGG 
GGGATACGAG GGGCAGGGGA TGCCAGTGTT GGTGTGTCGT CAGCCCCTGT CCACCGGAGG 
GACAGACAAG CCATCTCCAT GTACGAGCCT GGGGCAGCCC CACCCAAGCC ACTCTCCCCA 
GCCCTGGACC CCCTTGCCAG CCGCCTGCAG CCCCTACACC CCAGTGTGCG GAAAGGTGTG 
TCCACAGTTC CCGCCCCCTA CAGCAGCCTG CACCTCTCTG GATCAGCTGA AGTGGGCCGG 
TACATGGCCC CTAAGGGAGA AAAGCACACG AGTGGCACCG ACAGTGACTA TGACAACACT 
CAAACATATG ATCTGTCACT CAGTGTGGGC CGCAGCAGTG AGGAGGAGAG CCGAGCCGAC 
CTGGAGGAGG GGCCTAGTGA GGGTGGGGAA CCAGACCCCA CATTGCCGTG CACTGAGGAT 
GTCATCTTGA AGACAGAGCA GGTGACCAAG AATATTCAGG AACTACTTCG AGCCGCACAG 
GAGTTCAAAC ATGACAGTTT TGTACCGTGC TCAGAGAAGA TCCACTGTGC AGTCACAGAG 
ATGGCCTCGC TCTTTCCCAA GCGCCCGGCG CTGGATGCGG TACGTTGCTC CCTGCGCCTC 
TTGGCAGCCA GTGCCTCCCG GTTGCAGGGG GAGTGCCGTA AGGCTGTGCC AGCTGAGCCC 
TCAGCCACAC CCGTAGACTA CCAGCTCCTC ACTCAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCTAAGG CTGCCAAACA GCTGGTCACC ATTACAACCC GTGAGAAGAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603751.1
CDS	531..2807
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQMENSAMRGGQEGGTDVLGVGSGSGLWAGGIRGAGDASVGVSSAPVHRRDRQAISMYEPGAAPPKPLSPALDPLASRLQPLHPSVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748235.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748235.2

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GATCTCACGG ATGCAGATGG AGAACAGTGC GATGCGGGGG 
GGCCAGGAAG GGGGAACTGA CGTTTTGGGA GTGGGCAGTG GGTCAGGGCT TTGGGCTGGG 
GGGATACGAG GGGCAGGGGA TGCCAGTGTT GGTGTGTCGT CAGCCCCTGT CCACCGGAGG 
GACAGACAAG CCATCTCCAT GTACGAGCCT GGGGCAGCCC CACCCAAGCC ACTCTCCCCA 
GCCCTGGACC CCCTTGCCAG CCGCCTGCAG CCCCTACACC CCAGTGTGCG GAAAGGTGTG 
TCCACAGTTC CCGCCCCCTA CAGCAGCCTG CACCTCTCTG GATCAGCTGA AGTGGGCCGG 
TACATGGCCC CTAAGGGAGA AAAGCACACG AGTGGCACCG ACAGTGACTA TGACAACACT 
CAAACATATG ATCTGTCACT CAGTGTGGGC CGCAGCAGTG AGGAGGAGAG CCGAGCCGAC 
CTGGAGGAGG GGCCTAGTGA GGGTGGGGAA CCAGACCCCA CATTGCCGTG CACTGAGGAT 
GTCATCTTGA AGACAGAGCA GGTGACCAAG AATATTCAGG AACTACTTCG AGCCGCACAG 
GAGTTCAAAC ATGACAGTTT TGTACCGTGC TCAGAGAAGA TCCACTGTGC AGTCACAGAG 
ATGGCCTCGC TCTTTCCCAA GCGCCCGGCG CTGGATGCGG TACGTTGCTC CCTGCGCCTC 
TTGGCAGCCA GTGCCTCCCG GTTGCAGGGG GAGTGCCGTA AGGCTGTGCC AGCTGAGCCC 
TCAGCCACAC CCGTAGACTA CCAGCTCCTC ACTCAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCTAAGG CTGCCAAACA GCTGGTCACC ATTACAACCC GTGAGAAGAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_018748236.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603752.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSc144054
Clone ID Related Accession (Same CDS sequence)	XM_018748236.2
Accession Version	XM_018748236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018748236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GCCTGGACCT GTCGGAACTG 
GCCAAGGCAG CAAAGAAGAA ACTCCAAGCA CTCAACAACC GGCTGTTTGA AGAACTAGCC 
ATGGACGTGT ACGATGAGGT GGATCGCAGG GAAAATGATG CAGTGTGGCT GACAACACAG 
AACCACAGTA CACTGGTGAC AGAGCGCAGT GCTGTTCCTT TCCTACCAGT CAACCCAGAA 
TACTCCGCCA CACGAAATCA GGGTCGACAG AAGCTAGCTC GTTTTAATGC TCGGGAGTTT 
GCCACCTTGA TCATCGACAT TCTGAGTGAT GCCAAGAGAC GTCAGCAGGG CAAGGGACTC 
ACGAGCCCTG TGGATCCCTT GGATTTGGGT CATGGTGATG ATGACCAGCA TGACTATGAC 
AGTGTAGCAT CAGATGAGGA CACAGACAGC GAGCTGACAA CGCAGAACAA CACCAACGCC 
CAACGTAACA ATCGTGCCAA GAGTATGGAC TCCTCAGACC TTTCGGATGG GCCCATCACA 
TTACAGGAGT ATCTGGAGGT GAAAAGGGCT CTGGCTTCCT CTGAAGCCAA GGTACAGCAG 
CTCATGAAAG TCAACAACAA CCTGAGTGAG GAGCTCCGAC GTCTGCAGAA GGAGATCTCA 
CGGATGCAGA TGGAGAACAG TGCGATGCGG GGGGGCCAGG AAGGGGGAAC TGACGTTTTG 
GGAGTGGGCA GTGGGTCAGG GCTTTGGGCT GGGGGGATAC GAGGGGCAGG GGATGCCAGT 
GTTGGTGTGT CGTCAGCCCC TGTCCACCGG AGGGACAGAC AAGCCATCTC CATGTACGAG 
CCTGGGGCAG CCCCACCCAA GCCACTCTCC CCAGCCCTGG ACCCCCTTGC CAGCCGCCTG 
CAGCCCCTAC ACCCCAGTGT GCGGAAAGGT GTGTCCACAG TTCCCGCCCC CTACAGCAGC 
CTGCACCTCT CTGGATCAGC TGAAGTGGGC CGGTACATGG CCCCTAAGGG AGAAAAGCAC 
ACGAGTGGCA CCGACAGTGA CTATGACAAC ACTCAAACAT ATGATCTGTC ACTCAGTGTG 
GGCCGCAGCA GTGAGGAGGA GAGCCGAGCC GACCTGGAGG AGGGGCCTAG TGAGGGTGGG 
GAACCAGACC CCACATTGCC GTGCACTGAG GATGTCATCT TGAAGACAGA GCAGGTGACC 
AAGAATATTC AGGAACTACT TCGAGCCGCA CAGGAGTTCA AACATGACAG TTTTGTACCG 
TGCTCAGAGA AGATCCACTG TGCAGTCACA GAGATGGCCT CGCTCTTTCC CAAGCGCCCG 
GCGCTGGATG CGGTACGTTG CTCCCTGCGC CTCTTGGCAG CCAGTGCCTC CCGGTTGCAG 
GGGGAGTGCC GTAAGGCTGT GCCAGCTGAG CCCTCAGCCA CACCCGTAGA CTACCAGCTC 
CTCACTCAGC AGGTCATCCA GTGCGCCTAC GACATCGCTA AGGCTGCCAA ACAGCTGGTC 
ACCATTACAA CCCGTGAGAA GAAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603752.1
CDS	531..2780
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQMENSAMRGGQEGGTDVLGVGSGSGLWAGGIRGAGDASVGVSSAPVHRRDRQAISMYEPGAAPPKPLSPALDPLASRLQPLHPSVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748236.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748236.2

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GCCTGGACCT GTCGGAACTG 
GCCAAGGCAG CAAAGAAGAA ACTCCAAGCA CTCAACAACC GGCTGTTTGA AGAACTAGCC 
ATGGACGTGT ACGATGAGGT GGATCGCAGG GAAAATGATG CAGTGTGGCT GACAACACAG 
AACCACAGTA CACTGGTGAC AGAGCGCAGT GCTGTTCCTT TCCTACCAGT CAACCCAGAA 
TACTCCGCCA CACGAAATCA GGGTCGACAG AAGCTAGCTC GTTTTAATGC TCGGGAGTTT 
GCCACCTTGA TCATCGACAT TCTGAGTGAT GCCAAGAGAC GTCAGCAGGG CAAGGGACTC 
ACGAGCCCTG TGGATCCCTT GGATTTGGGT CATGGTGATG ATGACCAGCA TGACTATGAC 
AGTGTAGCAT CAGATGAGGA CACAGACAGC GAGCTGACAA CGCAGAACAA CACCAACGCC 
CAACGTAACA ATCGTGCCAA GAGTATGGAC TCCTCAGACC TTTCGGATGG GCCCATCACA 
TTACAGGAGT ATCTGGAGGT GAAAAGGGCT CTGGCTTCCT CTGAAGCCAA GGTACAGCAG 
CTCATGAAAG TCAACAACAA CCTGAGTGAG GAGCTCCGAC GTCTGCAGAA GGAGATCTCA 
CGGATGCAGA TGGAGAACAG TGCGATGCGG GGGGGCCAGG AAGGGGGAAC TGACGTTTTG 
GGAGTGGGCA GTGGGTCAGG GCTTTGGGCT GGGGGGATAC GAGGGGCAGG GGATGCCAGT 
GTTGGTGTGT CGTCAGCCCC TGTCCACCGG AGGGACAGAC AAGCCATCTC CATGTACGAG 
CCTGGGGCAG CCCCACCCAA GCCACTCTCC CCAGCCCTGG ACCCCCTTGC CAGCCGCCTG 
CAGCCCCTAC ACCCCAGTGT GCGGAAAGGT GTGTCCACAG TTCCCGCCCC CTACAGCAGC 
CTGCACCTCT CTGGATCAGC TGAAGTGGGC CGGTACATGG CCCCTAAGGG AGAAAAGCAC 
ACGAGTGGCA CCGACAGTGA CTATGACAAC ACTCAAACAT ATGATCTGTC ACTCAGTGTG 
GGCCGCAGCA GTGAGGAGGA GAGCCGAGCC GACCTGGAGG AGGGGCCTAG TGAGGGTGGG 
GAACCAGACC CCACATTGCC GTGCACTGAG GATGTCATCT TGAAGACAGA GCAGGTGACC 
AAGAATATTC AGGAACTACT TCGAGCCGCA CAGGAGTTCA AACATGACAG TTTTGTACCG 
TGCTCAGAGA AGATCCACTG TGCAGTCACA GAGATGGCCT CGCTCTTTCC CAAGCGCCCG 
GCGCTGGATG CGGTACGTTG CTCCCTGCGC CTCTTGGCAG CCAGTGCCTC CCGGTTGCAG 
GGGGAGTGCC GTAAGGCTGT GCCAGCTGAG CCCTCAGCCA CACCCGTAGA CTACCAGCTC 
CTCACTCAGC AGGTCATCCA GTGCGCCTAC GACATCGCTA AGGCTGCCAA ACAGCTGGTC 
ACCATTACAA CCCGTGAGAA GAAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_018748237.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603753.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSc144055
Clone ID Related Accession (Same CDS sequence)	XM_018748237.2
Accession Version	XM_018748237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018748237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GGTGCGGAAA GGTGTGTCCA CAGTTCCCGC CCCCTACAGC 
AGCCTGCACC TCTCTGGATC AGCTGAAGTG GGCCGGTACA TGGCCCCTAA GGGAGAAAAG 
CACACGAGTG GCACCGACAG TGACTATGAC AACACTCAAA CATATGATCT GTCACTCAGT 
GTGGGCCGCA GCAGTGAGGA GGAGAGCCGA GCCGACCTGG AGGAGGGGCC TAGTGAGGGT 
GGGGAACCAG ACCCCACATT GCCGTGCACT GAGGATGTCA TCTTGAAGAC AGAGCAGGTG 
ACCAAGAATA TTCAGGAACT ACTTCGAGCC GCACAGGAGT TCAAACATGA CAGTTTTGTA 
CCGTGCTCAG AGAAGATCCA CTGTGCAGTC ACAGAGATGG CCTCGCTCTT TCCCAAGCGC 
CCGGCGCTGG ATGCGGTACG TTGCTCCCTG CGCCTCTTGG CAGCCAGTGC CTCCCGGTTG 
CAGGGGGAGT GCCGTAAGGC TGTGCCAGCT GAGCCCTCAG CCACACCCGT AGACTACCAG 
CTCCTCACTC AGCAGGTCAT CCAGTGCGCC TACGACATCG CTAAGGCTGC CAAACAGCTG 
GTCACCATTA CAACCCGTGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603753.1
CDS	531..2543
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748237.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748237.2

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTTTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GGTGCGGAAA GGTGTGTCCA CAGTTCCCGC CCCCTACAGC 
AGCCTGCACC TCTCTGGATC AGCTGAAGTG GGCCGGTACA TGGCCCCTAA GGGAGAAAAG 
CACACGAGTG GCACCGACAG TGACTATGAC AACACTCAAA CATATGATCT GTCACTCAGT 
GTGGGCCGCA GCAGTGAGGA GGAGAGCCGA GCCGACCTGG AGGAGGGGCC TAGTGAGGGT 
GGGGAACCAG ACCCCACATT GCCGTGCACT GAGGATGTCA TCTTGAAGAC AGAGCAGGTG 
ACCAAGAATA TTCAGGAACT ACTTCGAGCC GCACAGGAGT TCAAACATGA CAGTTTTGTA 
CCGTGCTCAG AGAAGATCCA CTGTGCAGTC ACAGAGATGG CCTCGCTCTT TCCCAAGCGC 
CCGGCGCTGG ATGCGGTACG TTGCTCCCTG CGCCTCTTGG CAGCCAGTGC CTCCCGGTTG 
CAGGGGGAGT GCCGTAAGGC TGTGCCAGCT GAGCCCTCAG CCACACCCGT AGACTACCAG 
CTCCTCACTC AGCAGGTCAT CCAGTGCGCC TACGACATCG CTAAGGCTGC CAAACAGCTG 
GTCACCATTA CAACCCGTGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_018748235.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603751.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSc51745
Clone ID Related Accession (Same CDS sequence)	XM_018748235.1
Accession Version	XM_018748235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GATCTCACGG ATGCAGATGG AGAACAGTGC GATGCGGGGG 
GGCCAGGAAG GGGGAACTGA CGTTTTGGGA GTGGGCAGTG GGTCAGGGCT TTGGGCTGGG 
GGGATACGAG GGGCAGGGGA TGCCAGTGTT GGTGTGTCGT CAGCCCCTGT CCACCGGAGG 
GACAGACAAG CCATCTCCAT GTACGAGCCT GGGGCAGCCC CACCCAAGCC ACTCTCCCCA 
GCCCTGGACC CCCTTGCCAG CCGCCTGCAG CCCCTACACC CCAGTGTGCG GAAAGGTGTG 
TCCACAGTTC CCGCCCCCTA CAGCAGCCTG CACCTCTCTG GATCAGCTGA AGTGGGCCGG 
TACATGGCCC CTAAGGGAGA AAAGCACACG AGTGGCACCG ACAGTGACTA TGACAACACT 
CAAACATATG ATCTGTCACT CAGTGTGGGC CGCAGCAGTG AGGAGGAGAG CCGAGCCGAC 
CTGGAGGAGG GGCCTAGTGA GGGTGGGGAA CCAGACCCCA CATTGCCGTG CACTGAGGAT 
GTCATCTTGA AGACAGAGCA GGTGACCAAG AATATTCAGG AACTACTTCG AGCCGCACAG 
GAGTTCAAAC ATGACAGTTT TGTACCGTGC TCAGAGAAGA TCCACTGTGC AGTCACAGAG 
ATGGCCTCGC TCTTTCCCAA GCGCCCGGCG CTGGATGCGG TACGTTGCTC CCTGCGCCTC 
TTGGCAGCCA GTGCCTCCCG GTTGCAGGGG GAGTGCCGTA AGGCTGTGCC AGCTGAGCCC 
TCAGCCACAC CCGTAGACTA CCAGCTCCTC ACTCAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCTAAGG CTGCCAAACA GCTGGTCACC ATTACAACCC GTGAGAAGAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603751.1
CDS	163..2439
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQMENSAMRGGQEGGTDVLGVGSGSGLWAGGIRGAGDASVGVSSAPVHRRDRQAISMYEPGAAPPKPLSPALDPLASRLQPLHPSVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748235.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748235.1

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GATCTCACGG ATGCAGATGG AGAACAGTGC GATGCGGGGG 
GGCCAGGAAG GGGGAACTGA CGTTTTGGGA GTGGGCAGTG GGTCAGGGCT TTGGGCTGGG 
GGGATACGAG GGGCAGGGGA TGCCAGTGTT GGTGTGTCGT CAGCCCCTGT CCACCGGAGG 
GACAGACAAG CCATCTCCAT GTACGAGCCT GGGGCAGCCC CACCCAAGCC ACTCTCCCCA 
GCCCTGGACC CCCTTGCCAG CCGCCTGCAG CCCCTACACC CCAGTGTGCG GAAAGGTGTG 
TCCACAGTTC CCGCCCCCTA CAGCAGCCTG CACCTCTCTG GATCAGCTGA AGTGGGCCGG 
TACATGGCCC CTAAGGGAGA AAAGCACACG AGTGGCACCG ACAGTGACTA TGACAACACT 
CAAACATATG ATCTGTCACT CAGTGTGGGC CGCAGCAGTG AGGAGGAGAG CCGAGCCGAC 
CTGGAGGAGG GGCCTAGTGA GGGTGGGGAA CCAGACCCCA CATTGCCGTG CACTGAGGAT 
GTCATCTTGA AGACAGAGCA GGTGACCAAG AATATTCAGG AACTACTTCG AGCCGCACAG 
GAGTTCAAAC ATGACAGTTT TGTACCGTGC TCAGAGAAGA TCCACTGTGC AGTCACAGAG 
ATGGCCTCGC TCTTTCCCAA GCGCCCGGCG CTGGATGCGG TACGTTGCTC CCTGCGCCTC 
TTGGCAGCCA GTGCCTCCCG GTTGCAGGGG GAGTGCCGTA AGGCTGTGCC AGCTGAGCCC 
TCAGCCACAC CCGTAGACTA CCAGCTCCTC ACTCAGCAGG TCATCCAGTG CGCCTACGAC 
ATCGCTAAGG CTGCCAAACA GCTGGTCACC ATTACAACCC GTGAGAAGAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_018748236.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603752.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSc51746
Clone ID Related Accession (Same CDS sequence)	XM_018748236.1
Accession Version	XM_018748236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GCCTGGACCT GTCGGAACTG 
GCCAAGGCAG CAAAGAAGAA ACTCCAAGCA CTCAACAACC GGCTGTTTGA AGAACTAGCC 
ATGGACGTGT ACGATGAGGT GGATCGCAGG GAAAATGATG CAGTGTGGCT GACAACACAG 
AACCACAGTA CACTGGTGAC AGAGCGCAGT GCTGTTCCTT TCCTACCAGT CAACCCAGAA 
TACTCCGCCA CACGAAATCA GGGTCGACAG AAGCTAGCTC GTTTTAATGC TCGGGAGTTT 
GCCACCTTGA TCATCGACAT TCTGAGTGAT GCCAAGAGAC GTCAGCAGGG CAAGGGACTC 
ACGAGCCCTG TGGATCCCTT GGATTTGGGT CATGGTGATG ATGACCAGCA TGACTATGAC 
AGTGTAGCAT CAGATGAGGA CACAGACAGC GAGCTGACAA CGCAGAACAA CACCAACGCC 
CAACGTAACA ATCGTGCCAA GAGTATGGAC TCCTCAGACC TTTCGGATGG GCCCATCACA 
TTACAGGAGT ATCTGGAGGT GAAAAGGGCT CTGGCTTCCT CTGAAGCCAA GGTACAGCAG 
CTCATGAAAG TCAACAACAA CCTGAGTGAG GAGCTCCGAC GTCTGCAGAA GGAGATCTCA 
CGGATGCAGA TGGAGAACAG TGCGATGCGG GGGGGCCAGG AAGGGGGAAC TGACGTTTTG 
GGAGTGGGCA GTGGGTCAGG GCTTTGGGCT GGGGGGATAC GAGGGGCAGG GGATGCCAGT 
GTTGGTGTGT CGTCAGCCCC TGTCCACCGG AGGGACAGAC AAGCCATCTC CATGTACGAG 
CCTGGGGCAG CCCCACCCAA GCCACTCTCC CCAGCCCTGG ACCCCCTTGC CAGCCGCCTG 
CAGCCCCTAC ACCCCAGTGT GCGGAAAGGT GTGTCCACAG TTCCCGCCCC CTACAGCAGC 
CTGCACCTCT CTGGATCAGC TGAAGTGGGC CGGTACATGG CCCCTAAGGG AGAAAAGCAC 
ACGAGTGGCA CCGACAGTGA CTATGACAAC ACTCAAACAT ATGATCTGTC ACTCAGTGTG 
GGCCGCAGCA GTGAGGAGGA GAGCCGAGCC GACCTGGAGG AGGGGCCTAG TGAGGGTGGG 
GAACCAGACC CCACATTGCC GTGCACTGAG GATGTCATCT TGAAGACAGA GCAGGTGACC 
AAGAATATTC AGGAACTACT TCGAGCCGCA CAGGAGTTCA AACATGACAG TTTTGTACCG 
TGCTCAGAGA AGATCCACTG TGCAGTCACA GAGATGGCCT CGCTCTTTCC CAAGCGCCCG 
GCGCTGGATG CGGTACGTTG CTCCCTGCGC CTCTTGGCAG CCAGTGCCTC CCGGTTGCAG 
GGGGAGTGCC GTAAGGCTGT GCCAGCTGAG CCCTCAGCCA CACCCGTAGA CTACCAGCTC 
CTCACTCAGC AGGTCATCCA GTGCGCCTAC GACATCGCTA AGGCTGCCAA ACAGCTGGTC 
ACCATTACAA CCCGTGAGAA GAAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603752.1
CDS	163..2412
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQMENSAMRGGQEGGTDVLGVGSGSGLWAGGIRGAGDASVGVSSAPVHRRDRQAISMYEPGAAPPKPLSPALDPLASRLQPLHPSVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748236.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748236.1

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GCCTGGACCT GTCGGAACTG 
GCCAAGGCAG CAAAGAAGAA ACTCCAAGCA CTCAACAACC GGCTGTTTGA AGAACTAGCC 
ATGGACGTGT ACGATGAGGT GGATCGCAGG GAAAATGATG CAGTGTGGCT GACAACACAG 
AACCACAGTA CACTGGTGAC AGAGCGCAGT GCTGTTCCTT TCCTACCAGT CAACCCAGAA 
TACTCCGCCA CACGAAATCA GGGTCGACAG AAGCTAGCTC GTTTTAATGC TCGGGAGTTT 
GCCACCTTGA TCATCGACAT TCTGAGTGAT GCCAAGAGAC GTCAGCAGGG CAAGGGACTC 
ACGAGCCCTG TGGATCCCTT GGATTTGGGT CATGGTGATG ATGACCAGCA TGACTATGAC 
AGTGTAGCAT CAGATGAGGA CACAGACAGC GAGCTGACAA CGCAGAACAA CACCAACGCC 
CAACGTAACA ATCGTGCCAA GAGTATGGAC TCCTCAGACC TTTCGGATGG GCCCATCACA 
TTACAGGAGT ATCTGGAGGT GAAAAGGGCT CTGGCTTCCT CTGAAGCCAA GGTACAGCAG 
CTCATGAAAG TCAACAACAA CCTGAGTGAG GAGCTCCGAC GTCTGCAGAA GGAGATCTCA 
CGGATGCAGA TGGAGAACAG TGCGATGCGG GGGGGCCAGG AAGGGGGAAC TGACGTTTTG 
GGAGTGGGCA GTGGGTCAGG GCTTTGGGCT GGGGGGATAC GAGGGGCAGG GGATGCCAGT 
GTTGGTGTGT CGTCAGCCCC TGTCCACCGG AGGGACAGAC AAGCCATCTC CATGTACGAG 
CCTGGGGCAG CCCCACCCAA GCCACTCTCC CCAGCCCTGG ACCCCCTTGC CAGCCGCCTG 
CAGCCCCTAC ACCCCAGTGT GCGGAAAGGT GTGTCCACAG TTCCCGCCCC CTACAGCAGC 
CTGCACCTCT CTGGATCAGC TGAAGTGGGC CGGTACATGG CCCCTAAGGG AGAAAAGCAC 
ACGAGTGGCA CCGACAGTGA CTATGACAAC ACTCAAACAT ATGATCTGTC ACTCAGTGTG 
GGCCGCAGCA GTGAGGAGGA GAGCCGAGCC GACCTGGAGG AGGGGCCTAG TGAGGGTGGG 
GAACCAGACC CCACATTGCC GTGCACTGAG GATGTCATCT TGAAGACAGA GCAGGTGACC 
AAGAATATTC AGGAACTACT TCGAGCCGCA CAGGAGTTCA AACATGACAG TTTTGTACCG 
TGCTCAGAGA AGATCCACTG TGCAGTCACA GAGATGGCCT CGCTCTTTCC CAAGCGCCCG 
GCGCTGGATG CGGTACGTTG CTCCCTGCGC CTCTTGGCAG CCAGTGCCTC CCGGTTGCAG 
GGGGAGTGCC GTAAGGCTGT GCCAGCTGAG CCCTCAGCCA CACCCGTAGA CTACCAGCTC 
CTCACTCAGC AGGTCATCCA GTGCGCCTAC GACATCGCTA AGGCTGCCAA ACAGCTGGTC 
ACCATTACAA CCCGTGAGAA GAAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_018748237.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018603753.1 Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.

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CloneID	OSc51747
Clone ID Related Accession (Same CDS sequence)	XM_018748237.1
Accession Version	XM_018748237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GGTGCGGAAA GGTGTGTCCA CAGTTCCCGC CCCCTACAGC 
AGCCTGCACC TCTCTGGATC AGCTGAAGTG GGCCGGTACA TGGCCCCTAA GGGAGAAAAG 
CACACGAGTG GCACCGACAG TGACTATGAC AACACTCAAA CATATGATCT GTCACTCAGT 
GTGGGCCGCA GCAGTGAGGA GGAGAGCCGA GCCGACCTGG AGGAGGGGCC TAGTGAGGGT 
GGGGAACCAG ACCCCACATT GCCGTGCACT GAGGATGTCA TCTTGAAGAC AGAGCAGGTG 
ACCAAGAATA TTCAGGAACT ACTTCGAGCC GCACAGGAGT TCAAACATGA CAGTTTTGTA 
CCGTGCTCAG AGAAGATCCA CTGTGCAGTC ACAGAGATGG CCTCGCTCTT TCCCAAGCGC 
CCGGCGCTGG ATGCGGTACG TTGCTCCCTG CGCCTCTTGG CAGCCAGTGC CTCCCGGTTG 
CAGGGGGAGT GCCGTAAGGC TGTGCCAGCT GAGCCCTCAG CCACACCCGT AGACTACCAG 
CTCCTCACTC AGCAGGTCAT CCAGTGCGCC TACGACATCG CTAAGGCTGC CAAACAGCTG 
GTCACCATTA CAACCCGTGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018603753.1
CDS	163..2175
Translation	MSRKVQRSEVCADCSAPDPGWTSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPTLLQMVQMLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDINGRTPMDYARQAGQVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQQGKGLTSPVDPLDLGHGDDDQHDYDSVASDEDTDSELTTQNNTNAQRNNRAKSMDSSDLSDGPITLQEYLEVKRALASSEAKVQQLMKVNNNLSEELRRLQKEVRKGVSTVPAPYSSLHLSGSAEVGRYMAPKGEKHTSGTDSDYDNTQTYDLSLSVGRSSEEESRADLEEGPSEGGEPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHCAVTEMASLFPKRPALDAVRCSLRLLAASASRLQGECRKAVPAEPSATPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018748237.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus GIT ArfGAP 1 (git1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018748237.1

ORF Insert Sequence:

ATGTCCAGGA AAGTGCAGAG GAGTGAGGTG TGCGCCGACT GCAGCGCCCC AGACCCTGGC 
TGGACGAGTA TTAACCGGGG GGTGCTGATC TGTGACGAGT GCTGTTCTGT TCACCGCAGT 
CTGGGTCGCC ATATCTCCAT TGTCAAACAC CTTCGACACA GCGGTTGGCC ACCCACATTG 
CTGCAGATGG TACAGATGTT GGCCAGCAAT GGGGCAAACT CCATCTGGGA GCACTCCCTG 
CTGGACCCTG CTCAAGTCCA AAGTGGCCGC AGGAAACCCA ATCCCCAGGA CAAAGTGCAC 
CCCACCAAGT CAGAGTTCAT TCGGGCCAAG TACCAGATGC TGGCATTTGT GCACAAGTTG 
CCTTGTCGTG ATGACGACGG TGTTACCACC AAGGATCTCA GCAAGCAATT GCACTCCAGC 
GTGCGAACTG GTAGCTTGGA AACCTGTCTG CGGCTCCTGT CCCTGGGTGC GCAGGCCAAC 
TTCTTCCACC CGGAGAAGGG CACAACCCCT CTGCATGTGG CTGCCAAGGC AGGGCAAATC 
CTCCAGGCAG AGCTGCTGGT GGTATATGGT GCTGACCCTG GAGCCCCTGA CATCAATGGT 
CGTACACCCA TGGACTATGC CAGGCAGGCA GGGCAGGTAG AGCTGGCCGA GCGACTTGTC 
GAGTGCCAGT ATGAACTCAC TGACAGGCTG GCATTCTATC TGTGCGGCCG ACGCCCGGAT 
CACAAAAATG GCCATTACAT CATACCACAG ATGGCAGACA GAGCTCGCCC CAAGTGTCCG 
ACGCAGAGCC TGGACCTGTC GGAACTGGCC AAGGCAGCAA AGAAGAAACT CCAAGCACTC 
AACAACCGGC TGTTTGAAGA ACTAGCCATG GACGTGTACG ATGAGGTGGA TCGCAGGGAA 
AATGATGCAG TGTGGCTGAC AACACAGAAC CACAGTACAC TGGTGACAGA GCGCAGTGCT 
GTTCCTTTCC TACCAGTCAA CCCAGAATAC TCCGCCACAC GAAATCAGGG TCGACAGAAG 
CTAGCTCGTT TTAATGCTCG GGAGTTTGCC ACCTTGATCA TCGACATTCT GAGTGATGCC 
AAGAGACGTC AGCAGGGCAA GGGACTCACG AGCCCTGTGG ATCCCTTGGA TTTGGGTCAT 
GGTGATGATG ACCAGCATGA CTATGACAGT GTAGCATCAG ATGAGGACAC AGACAGCGAG 
CTGACAACGC AGAACAACAC CAACGCCCAA CGTAACAATC GTGCCAAGAG TATGGACTCC 
TCAGACCTTT CGGATGGGCC CATCACATTA CAGGAGTATC TGGAGGTGAA AAGGGCTCTG 
GCTTCCTCTG AAGCCAAGGT ACAGCAGCTC ATGAAAGTCA ACAACAACCT GAGTGAGGAG 
CTCCGACGTC TGCAGAAGGA GGTGCGGAAA GGTGTGTCCA CAGTTCCCGC CCCCTACAGC 
AGCCTGCACC TCTCTGGATC AGCTGAAGTG GGCCGGTACA TGGCCCCTAA GGGAGAAAAG 
CACACGAGTG GCACCGACAG TGACTATGAC AACACTCAAA CATATGATCT GTCACTCAGT 
GTGGGCCGCA GCAGTGAGGA GGAGAGCCGA GCCGACCTGG AGGAGGGGCC TAGTGAGGGT 
GGGGAACCAG ACCCCACATT GCCGTGCACT GAGGATGTCA TCTTGAAGAC AGAGCAGGTG 
ACCAAGAATA TTCAGGAACT ACTTCGAGCC GCACAGGAGT TCAAACATGA CAGTTTTGTA 
CCGTGCTCAG AGAAGATCCA CTGTGCAGTC ACAGAGATGG CCTCGCTCTT TCCCAAGCGC 
CCGGCGCTGG ATGCGGTACG TTGCTCCCTG CGCCTCTTGG CAGCCAGTGC CTCCCGGTTG 
CAGGGGGAGT GCCGTAAGGC TGTGCCAGCT GAGCCCTCAG CCACACCCGT AGACTACCAG 
CTCCTCACTC AGCAGGTCAT CCAGTGCGCC TACGACATCG CTAAGGCTGC CAAACAGCTG 
GTCACCATTA CAACCCGTGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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git1 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム