WNK1 gene cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette)

mRNA	Protein	Name
XM_016131738.1	XP_015987224.1	serine/threonine-protein kinase WNK1 isoform X2
XM_016131743.1	XP_015987229.1	serine/threonine-protein kinase WNK1 isoform X6
XM_016131745.1	XP_015987231.1	serine/threonine-protein kinase WNK1 isoform X8
XM_016131741.1	XP_015987227.1	serine/threonine-protein kinase WNK1 isoform X5
XM_016131749.1	XP_015987235.1	serine/threonine-protein kinase WNK1 isoform X12
XM_016131750.1	XP_015987236.1	serine/threonine-protein kinase WNK1 isoform X13
XM_016131752.1	XP_015987238.1	serine/threonine-protein kinase WNK1 isoform X15
XM_016131753.1	XP_015987239.1	serine/threonine-protein kinase WNK1 isoform X16
XM_016131756.1	XP_015987242.1	serine/threonine-protein kinase WNK1 isoform X19
XM_016131759.1	XP_015987245.1	serine/threonine-protein kinase WNK1 isoform X21
XM_016131740.1	XP_015987226.1	serine/threonine-protein kinase WNK1 isoform X4
XM_016131754.1	XP_015987240.1	serine/threonine-protein kinase WNK1 isoform X17
XM_016131757.1	XP_015987243.1	serine/threonine-protein kinase WNK1 isoform X20
XM_016131737.1	XP_015987223.1	serine/threonine-protein kinase WNK1 isoform X1
XM_016131744.1	XP_015987230.1	serine/threonine-protein kinase WNK1 isoform X7
XM_016131748.1	XP_015987234.1	serine/threonine-protein kinase WNK1 isoform X11
XM_016131755.1	XP_015987241.1	serine/threonine-protein kinase WNK1 isoform X18
XM_016131739.1	XP_015987225.1	serine/threonine-protein kinase WNK1 isoform X3
XM_016131746.1	XP_015987232.1	serine/threonine-protein kinase WNK1 isoform X9
XM_016131747.1	XP_015987233.1	serine/threonine-protein kinase WNK1 isoform X10
XM_016131751.1	XP_015987237.1	serine/threonine-protein kinase WNK1 isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORo36507	XM_016131757.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ORo36488	XM_016131737.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36489	XM_016131738.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36490	XM_016131739.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36491	XM_016131740.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36492	XM_016131741.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36493	XM_016131743.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36494	XM_016131744.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36495	XM_016131745.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36496	XM_016131746.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ORo36497	XM_016131747.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36498	XM_016131748.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36499	XM_016131749.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36500	XM_016131750.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36501	XM_016131751.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36502	XM_016131752.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36503	XM_016131753.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36504	XM_016131754.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36505	XM_016131755.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36506	XM_016131756.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ORo36508	XM_016131759.1 Latest version!	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

WNK1 ( XM_016131757.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987243.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ORo36507
Clone ID Related Accession (Same CDS sequence)	XM_016131757.1
Accession Version	XM_016131757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTA CAAAGGAAGA TTTTTGCTCA GTGTTTGTCA TTGTAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGGGTGAAAC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGTTGCATTC GGCAAAACAA AGATGAAAGA 
TACTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTGCGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC 
CTGGAGAGGG ATGTGCCAGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTATGTCTGT 
GAAGGTGATC ACAAGACTAT GGCAAAAGCC ATCAAAGATA GAGTGTCTTT AATTAAGAGA 
AAACGAGAGC AGCGGCAATT GGTGCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGAGC 
AGTCTCAAAC AGCAAGTAGA ACAGCAACCA AGTGCTTCCC AGGCAGGAAT CAAGCAGCTC 
TCTTCTGCTA GCACCGGCAT ACCTACTGCC TCTGCCACTT CAGCTTCAGT TTCTACACAA 
GTAGAACCTG AAGAACCTGA GGCAGACCAA CATCAACAAC TACAGTACCA ACAACCTAGT 
GTATCTGTGT TATCCGATGG GACTGTTGAC AGTGGTCAGG GATCTTCTGT CTTCACAGAA 
TCTCGAGTCA GCAGCCAACA GACAGTTTCA TATGGTTCCC AACATGAACA GGCACATTCT 
ACTGGCACAT TCCCAGGGCA TACAGCTTCT GTTGTCCAAG CACAATCTCA GCCCCATGGT 
GTATATCCAC CCTCAAGTAT GGCACAGGGG CAGAGCCAGG GTCAGCCATC CTCAAGTAGT 
GTAACAGGGG TTTCATCTTC CCAATCCATA CAACATTCTC AGCAGCAGCA TGGAATACAA 
CAGACAGCCC CTCCTCAACA GACAGTGCAG TATTCACTTC CACAGACATC AACCTCCAGT 
GAGGCCACTA CTGCACAGCC AGTGAGTCAG CCTCAAGCTC CACAGGTCTT GCCTCAAGCA 
TCAGCTGGAA AACAGCTTCC AGTTTCCCAG CCAGTACCAA CTGTCCAAGG CGAACCTCAG 
ATCTCAGTTG CGACACAACC CTCAGTTGTT CCAGTGCATT CTGGTGCTCA TTTCCTCCCT 
ATGGGACAGC CACTTCCTAC TTCCTTACTC CCTCAGTACC CTGCCTCTCA AATTCCTGTG 
TCAACTCCTC ATGTGTCCAC GGCTCAGACA GGTTTCTCAA CCCTTCCCAT CACAATTGCA 
GCTGGCATTA ATCAGCCTCT GCTCACCTTG GCTTCATCTG CTACAGCAGC TGCAATCCCA 
GGGGGATCAA CTGTGGCTCC TAGTCAGCTA CCAGCCCTTC TGCAGTCTGT GACTCAGCTG 
CCAAGTCAGG TTCACCCACA GCTTCTGCAG CCAGCAGTTC AGTCCATGGG AATACCAGCT 
AACCTTGGAC AAGCTGCCGA GGTTCCACTT CCCTCTGGAG ATGTTCTATA CCAGGGCTTC 
CCACCTCGAC TGCCACCACA ATACCCAGGA GATTCAAATA TTGCTTCCTC TTCCAGCGTG 
GCTTCTGTTT CCATTCCTTC TACAGTCCTA TCCCCTCCAA TGCCGACAGA AGCATTAGCT 
ACACCAGGTT ACTTTCCTAC AGTGGTGCAG CCTTATATGG AATCAAATCT TATGGTTCCT 
GTGGGCAGTA TAGGAGGACA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AGCACAAGCC 
CCCACTGCAT CCTCCCAGCA AGCAGCTCTG GAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTCCAGAGC CAGTTTCGGT AGCACAGCCA CAGCCCACCC AGCCTGCCGC TTTGGTTTCT 
TCTATAGACA GTGCTCATTC AGATGTCGCT TCGGGTATGA GCGATGGCAA TGAGAATGTC 
CCATCATCTG GTGGAAGGCA TGAAGGGAGA ACTGCAAAAC GGCATTATCG AAAATCTGTA 
AGAAGCCGCT CTCGTCATGA AAAGACTTCA CGCCCCAAAT TGAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGGGTAGT AGAATGTCAA TTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGGGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CTGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AGGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAGGGAGAA 
TTCAAACAAC CACTTCCTGC ATCTTCCATG CCACAGCAAA TAGGAATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCCGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCACCAGT GAAATGTCAG ATACAGTTGC CGCCTCTACA 
TCTCATGGCG CTGGGGTGAA CTTGTCCCAC TCTGCTTCTT CCCTTAGTCT ACAGCAGGCC 
TTTTCTGAAC TCAGACACGC CCAAGTGACA GAGGGACCCA ATACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAAAGACAA CAGCAGCCGC ACCTTCAATA TCAGTCCCTG TAACTAGCTG CCCTCTTAAT 
GACGTTTCCA CGTCAATAAT TCAGCCTGAG ATTACAGTGC CCACTGAAAA AGGAGTTGCT 
GGAGTTGCCA CCTGCACAGG TGTAATAACT TCAAGTGGTT TCCCAGTACC CCCTGCATCT 
GAATCACCAG TTCTTTCCAG TGTGGTTTCA AGTATCACAA TACCGACAAT TGTCTCAATA 
TCAACAACAT CCCAGTCAGT TCAGGCTCCC ACATCTGGGA GCGTTGTTTC TAGTGTAGGC 
ACTTTCCCTT CTATACCAGT TTCAGCAACT TCAGCCTCTG CAGTGGGCGG TGCTGCTACC 
CCAGGGGCTA AGTCTCCACC TCTATTATCT CAGCAGTCAG CAGGCATTAC TACAGGGATA 
GCCACATTAA CATCAGTTTC TACCACCATT CCATTTCCAA GCACAGCTTC ACAGCTATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCATTAG ATAAAACATC TCATAGCAGT ACAGCTGGAT TGGCTTTGTC CCACTCTGCA 
TCATCTTCCA CTTCTTCCCC TGGAGCAGGA GTATCTAGTT CTATTTCTCA GTCTGGTGGG 
GTACATCCTT TGGTCGTCCC ATCAGCTATA GCTTCTACTC CTGTCCTTCC CCAGGCATCA 
GTACCTATTT CTACACCTTT ATTACCCCAA GGACCTAGCA TCCCACCATT GGTGCAACCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC ACACTGCCCT GAAATAGATG TTGATACACA ACCCAAAGCT 
CCTGGGATTG ATGACATAAA GACTTTAGAG GAAAAGTTGC GTTCCCTCTT TAGTGAACAC 
AGCTCTTCTG GCGCTCAACA TGCCTCTGTT GCACTGGAGA CATCACTAGT AGTAGAGACT 
AGTGTCACAC CAGGCATCCC AACCACTGCT GTTGCACCCA GCAAACTCAT GACTTCAACT 
ACAAGTACTT GCTTACCACC AACCAGTTTG CCATTAGGAA CAGCTGGTTT GTCCGTTACA 
CCACTGGTCA CAACTGGGCA AGTTTCTACC CCCGTCGGCA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CATCAAAGCT ACCTTTAACT AAGGCTCCAG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCTG GTACTCCATC CAGTGAGCAG CTGCCACCTA TTCCAGGACC TTCACTAACT 
CAGTCCCAGC AACCTCTGGA AGATCTTGAT GCTCAGTTAA GAAGAGCACT TAGTCCAGAG 
ACTATTGCGG TGACATCCGC AGTTGGTCCT GTGTCCGTGG TGGCTCCAGC GGCAGTTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA CAGGAGGTTC TGTCCCAGCA 
ACTAGTTCAG GAACTGGTGT TTTTAAGACG GGGCGATTTC AGGTTTCAGT TGCAATAGAT 
GATTCCCAAA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCCT GGTGAAGCCA 
GGCTCTAATG GGGTAACCAT CCATGGTATT TCTTTAGATA TGCCAGATAG TGCCCACAAA 
ACTCCTGCTG CAGAAGCAAA GTCAGAAACT GGGCAACCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTAGGTCGT TTCTCTGTAT CAAGAACTGA AGACAGGATC 
ACTGAGGCAA AGAAAGAGGG ACCGACGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGCGATACC AAAGAAAGAA AAACCTGAAC TGTCAGAGCC TTCACATCTG 
AACGGGCCAT CTTCTGATTT GGAGGCCGCC TTCCTAAGTA GGGATGTAGA TGATGGTTCA 
GGTAGTCCAC ACTCTCCTCA CCAACTGAGC TCAAAGAGTC TACCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAACTCACT CAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATT 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ACAAACATCT TAAAGAGATT 
CAAGACCTAC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG 
GTTCCTCCTG CTGTCATTAT TCCCCCAGCG GCCCCCCTTT CAGGAAGAAG AAGGCGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGT ATCAGTTCCT TGGGGAGTAA AAGCCCCCAG 
CTTTCAGGTA ATATGTCTGG TCAGAGTGAA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCCG GCAACATCCC AGAAACTGGG CAGAATCAGT TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT GTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987243.1
CDS	6881..12808
Translation	MDFTKEDFCSVFVIVNSHCCCCPQKDCVNEGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131757.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131757.1

ORF Insert Sequence:

ATGGATTTTA CAAAGGAAGA TTTTTGCTCA GTGTTTGTCA TTGTAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGGGTGAAAC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGTTGCATTC GGCAAAACAA AGATGAAAGA 
TACTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTGCGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC 
CTGGAGAGGG ATGTGCCAGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTATGTCTGT 
GAAGGTGATC ACAAGACTAT GGCAAAAGCC ATCAAAGATA GAGTGTCTTT AATTAAGAGA 
AAACGAGAGC AGCGGCAATT GGTGCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGAGC 
AGTCTCAAAC AGCAAGTAGA ACAGCAACCA AGTGCTTCCC AGGCAGGAAT CAAGCAGCTC 
TCTTCTGCTA GCACCGGCAT ACCTACTGCC TCTGCCACTT CAGCTTCAGT TTCTACACAA 
GTAGAACCTG AAGAACCTGA GGCAGACCAA CATCAACAAC TACAGTACCA ACAACCTAGT 
GTATCTGTGT TATCCGATGG GACTGTTGAC AGTGGTCAGG GATCTTCTGT CTTCACAGAA 
TCTCGAGTCA GCAGCCAACA GACAGTTTCA TATGGTTCCC AACATGAACA GGCACATTCT 
ACTGGCACAT TCCCAGGGCA TACAGCTTCT GTTGTCCAAG CACAATCTCA GCCCCATGGT 
GTATATCCAC CCTCAAGTAT GGCACAGGGG CAGAGCCAGG GTCAGCCATC CTCAAGTAGT 
GTAACAGGGG TTTCATCTTC CCAATCCATA CAACATTCTC AGCAGCAGCA TGGAATACAA 
CAGACAGCCC CTCCTCAACA GACAGTGCAG TATTCACTTC CACAGACATC AACCTCCAGT 
GAGGCCACTA CTGCACAGCC AGTGAGTCAG CCTCAAGCTC CACAGGTCTT GCCTCAAGCA 
TCAGCTGGAA AACAGCTTCC AGTTTCCCAG CCAGTACCAA CTGTCCAAGG CGAACCTCAG 
ATCTCAGTTG CGACACAACC CTCAGTTGTT CCAGTGCATT CTGGTGCTCA TTTCCTCCCT 
ATGGGACAGC CACTTCCTAC TTCCTTACTC CCTCAGTACC CTGCCTCTCA AATTCCTGTG 
TCAACTCCTC ATGTGTCCAC GGCTCAGACA GGTTTCTCAA CCCTTCCCAT CACAATTGCA 
GCTGGCATTA ATCAGCCTCT GCTCACCTTG GCTTCATCTG CTACAGCAGC TGCAATCCCA 
GGGGGATCAA CTGTGGCTCC TAGTCAGCTA CCAGCCCTTC TGCAGTCTGT GACTCAGCTG 
CCAAGTCAGG TTCACCCACA GCTTCTGCAG CCAGCAGTTC AGTCCATGGG AATACCAGCT 
AACCTTGGAC AAGCTGCCGA GGTTCCACTT CCCTCTGGAG ATGTTCTATA CCAGGGCTTC 
CCACCTCGAC TGCCACCACA ATACCCAGGA GATTCAAATA TTGCTTCCTC TTCCAGCGTG 
GCTTCTGTTT CCATTCCTTC TACAGTCCTA TCCCCTCCAA TGCCGACAGA AGCATTAGCT 
ACACCAGGTT ACTTTCCTAC AGTGGTGCAG CCTTATATGG AATCAAATCT TATGGTTCCT 
GTGGGCAGTA TAGGAGGACA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AGCACAAGCC 
CCCACTGCAT CCTCCCAGCA AGCAGCTCTG GAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTCCAGAGC CAGTTTCGGT AGCACAGCCA CAGCCCACCC AGCCTGCCGC TTTGGTTTCT 
TCTATAGACA GTGCTCATTC AGATGTCGCT TCGGGTATGA GCGATGGCAA TGAGAATGTC 
CCATCATCTG GTGGAAGGCA TGAAGGGAGA ACTGCAAAAC GGCATTATCG AAAATCTGTA 
AGAAGCCGCT CTCGTCATGA AAAGACTTCA CGCCCCAAAT TGAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGGGTAGT AGAATGTCAA TTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGGGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CTGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AGGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAGGGAGAA 
TTCAAACAAC CACTTCCTGC ATCTTCCATG CCACAGCAAA TAGGAATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCCGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCACCAGT GAAATGTCAG ATACAGTTGC CGCCTCTACA 
TCTCATGGCG CTGGGGTGAA CTTGTCCCAC TCTGCTTCTT CCCTTAGTCT ACAGCAGGCC 
TTTTCTGAAC TCAGACACGC CCAAGTGACA GAGGGACCCA ATACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAAAGACAA CAGCAGCCGC ACCTTCAATA TCAGTCCCTG TAACTAGCTG CCCTCTTAAT 
GACGTTTCCA CGTCAATAAT TCAGCCTGAG ATTACAGTGC CCACTGAAAA AGGAGTTGCT 
GGAGTTGCCA CCTGCACAGG TGTAATAACT TCAAGTGGTT TCCCAGTACC CCCTGCATCT 
GAATCACCAG TTCTTTCCAG TGTGGTTTCA AGTATCACAA TACCGACAAT TGTCTCAATA 
TCAACAACAT CCCAGTCAGT TCAGGCTCCC ACATCTGGGA GCGTTGTTTC TAGTGTAGGC 
ACTTTCCCTT CTATACCAGT TTCAGCAACT TCAGCCTCTG CAGTGGGCGG TGCTGCTACC 
CCAGGGGCTA AGTCTCCACC TCTATTATCT CAGCAGTCAG CAGGCATTAC TACAGGGATA 
GCCACATTAA CATCAGTTTC TACCACCATT CCATTTCCAA GCACAGCTTC ACAGCTATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCATTAG ATAAAACATC TCATAGCAGT ACAGCTGGAT TGGCTTTGTC CCACTCTGCA 
TCATCTTCCA CTTCTTCCCC TGGAGCAGGA GTATCTAGTT CTATTTCTCA GTCTGGTGGG 
GTACATCCTT TGGTCGTCCC ATCAGCTATA GCTTCTACTC CTGTCCTTCC CCAGGCATCA 
GTACCTATTT CTACACCTTT ATTACCCCAA GGACCTAGCA TCCCACCATT GGTGCAACCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC ACACTGCCCT GAAATAGATG TTGATACACA ACCCAAAGCT 
CCTGGGATTG ATGACATAAA GACTTTAGAG GAAAAGTTGC GTTCCCTCTT TAGTGAACAC 
AGCTCTTCTG GCGCTCAACA TGCCTCTGTT GCACTGGAGA CATCACTAGT AGTAGAGACT 
AGTGTCACAC CAGGCATCCC AACCACTGCT GTTGCACCCA GCAAACTCAT GACTTCAACT 
ACAAGTACTT GCTTACCACC AACCAGTTTG CCATTAGGAA CAGCTGGTTT GTCCGTTACA 
CCACTGGTCA CAACTGGGCA AGTTTCTACC CCCGTCGGCA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CATCAAAGCT ACCTTTAACT AAGGCTCCAG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCTG GTACTCCATC CAGTGAGCAG CTGCCACCTA TTCCAGGACC TTCACTAACT 
CAGTCCCAGC AACCTCTGGA AGATCTTGAT GCTCAGTTAA GAAGAGCACT TAGTCCAGAG 
ACTATTGCGG TGACATCCGC AGTTGGTCCT GTGTCCGTGG TGGCTCCAGC GGCAGTTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA CAGGAGGTTC TGTCCCAGCA 
ACTAGTTCAG GAACTGGTGT TTTTAAGACG GGGCGATTTC AGGTTTCAGT TGCAATAGAT 
GATTCCCAAA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCCT GGTGAAGCCA 
GGCTCTAATG GGGTAACCAT CCATGGTATT TCTTTAGATA TGCCAGATAG TGCCCACAAA 
ACTCCTGCTG CAGAAGCAAA GTCAGAAACT GGGCAACCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTAGGTCGT TTCTCTGTAT CAAGAACTGA AGACAGGATC 
ACTGAGGCAA AGAAAGAGGG ACCGACGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGCGATACC AAAGAAAGAA AAACCTGAAC TGTCAGAGCC TTCACATCTG 
AACGGGCCAT CTTCTGATTT GGAGGCCGCC TTCCTAAGTA GGGATGTAGA TGATGGTTCA 
GGTAGTCCAC ACTCTCCTCA CCAACTGAGC TCAAAGAGTC TACCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAACTCACT CAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATT 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ACAAACATCT TAAAGAGATT 
CAAGACCTAC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG 
GTTCCTCCTG CTGTCATTAT TCCCCCAGCG GCCCCCCTTT CAGGAAGAAG AAGGCGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGT ATCAGTTCCT TGGGGAGTAA AAGCCCCCAG 
CTTTCAGGTA ATATGTCTGG TCAGAGTGAA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCCG GCAACATCCC AGAAACTGGG CAGAATCAGT TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT GTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131737.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987223.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	ORo36488
Clone ID Related Accession (Same CDS sequence)	XM_016131737.1
Accession Version	XM_016131737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCAGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCAAGCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CTGATGACCT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA CGCCATGAAT CTTTCAGGCA GGAGAGGAAG CAAAGGACAC 
ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGTCA GCTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCACC TACCCCCTTT 
GGCACTCAGT GGAGTGGGAC AGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGTT CCAACCTGAG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987223.1
CDS	656..7945
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131737.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131737.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCAGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCAAGCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CTGATGACCT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA CGCCATGAAT CTTTCAGGCA GGAGAGGAAG CAAAGGACAC 
ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGTCA GCTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCACC TACCCCCTTT 
GGCACTCAGT GGAGTGGGAC AGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGTT CCAACCTGAG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131738.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987224.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	ORo36489
Clone ID Related Accession (Same CDS sequence)	XM_016131738.1
Accession Version	XM_016131738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGCCT CTCAAATTCC TGTGTCAACT CCTCATGTGT CCACGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC ATTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCTGCAAT CCCAGGGGGA TCAACTGTGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGT CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTTCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CCGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTT CCTCTTCCAG CGTGGCTTCT GTTTCCATTC CTTCTACAGT CCTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTATGGT TCCTGTGGGC AGTATAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCGCTTTGGT TTCTTCTATA GACAGTGCTC ATTCAGATGT CGCTTCGGGT 
ATGAGCGATG GCAATGAGAA TGTCCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGC CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAATTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCATTTGTG 
GATCAAGTGC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCTGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAGGG AGAATTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGAA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCCGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGCCGATTA CGAGAATCAA AAGTTTTCAC CAGTGAAATG 
TCAGATACAG TTGCCGCCTC TACATCTCAT GGCGCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTCAGAC ACGCCCAAGT GACAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ACAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AGTCCCAAAG ACAACAGCAG CCGCACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACGTT TCCACGTCAA TAATTCAGCC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCCCCTGC ATCTGAATCA CCAGTTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCGA CAATTGTCTC AATATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTGT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCGGTGCTGC TACCCCAGGG GCTAAGTCTC CACCTCTATT ATCTCAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CATTCCATTT 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA TTAGATAAAA CATCTCATAG CAGTACAGCT 
GGATTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAGTCTGG TGGGGTACAT CCTTTGGTCG TCCCATCAGC TATAGCTTCT 
ACTCCTGTCC TTCCCCAGGC ATCAGTACCT ATTTCTACAC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCCC AACCAGCCCC ATACACACTG CCCTGAAATA 
GATGTTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTTT AGAGGAAAAG 
TTGCGTTCCC TCTTTAGTGA ACACAGCTCT TCTGGCGCTC AACATGCCTC TGTTGCACTG 
GAGACATCAC TAGTAGTAGA GACTAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCGT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCCGTC 
GGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGACTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGGAG GTTCTGTCCC AGCAACTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAAAAAGAGG GTAAAAATAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGGCTCT AATGGGGTAA CCATCCATGG TATTTCTTTA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCTGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGACGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GATCACTGAG GCAAAGAAAG AGGGACCGAC GGCATCTCCT 
CCTTTTATGG ATTTGGAACA AGCTGTTCTT CCTGCTGCGA TACCAAAGAA AGAAAAACCT 
GAACTGTCAG AGCCTTCACA TCTGAACGGG CCATCTTCTG ATTTGGAGGC CGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCTC CTCACCAACT GAGCTCAAAG 
AGTCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTACATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA GATTCAAGAC CTACAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTGCACC CCCAGCAGAC CCTCCACCCT CCCGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT 
AACTGTGCAT CAGAGCAGGT GACATTCAAA CCTGGTGGCA GGAGGACCCG ATTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT 
AGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACTG ATGACCTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGACGC CATGAATCTT TCAGGCAGGA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCACCTAC CCCCTTTGGC 
ACTCAGTGGA GTGGGACAGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987224.1
CDS	656..7942
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131738.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131738.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGC TTCCAGTTTC CCAGCCAGTA 
CCAACTGTCC AAGGCGAACC TCAGATCTCA GTTGCGACAC AACCCTCAGT TGTTCCAGTG 
CATTCTGGTG CTCATTTCCT CCCTATGGGA CAGCCACTTC CTACTTCCTT ACTCCCTCAG 
TACCCTGCCT CTCAAATTCC TGTGTCAACT CCTCATGTGT CCACGGCTCA GACAGGTTTC 
TCAACCCTTC CCATCACAAT TGCAGCTGGC ATTAATCAGC CTCTGCTCAC CTTGGCTTCA 
TCTGCTACAG CAGCTGCAAT CCCAGGGGGA TCAACTGTGG CTCCTAGTCA GCTACCAGCC 
CTTCTGCAGT CTGTGACTCA GCTGCCAAGT CAGGTTCACC CACAGCTTCT GCAGCCAGCA 
GTTCAGTCCA TGGGAATACC AGCTAACCTT GGACAAGCTG CCGAGGTTCC ACTTCCCTCT 
GGAGATGTTC TATACCAGGG CTTCCCACCT CGACTGCCAC CACAATACCC AGGAGATTCA 
AATATTGCTT CCTCTTCCAG CGTGGCTTCT GTTTCCATTC CTTCTACAGT CCTATCCCCT 
CCAATGCCGA CAGAAGCATT AGCTACACCA GGTTACTTTC CTACAGTGGT GCAGCCTTAT 
ATGGAATCAA ATCTTATGGT TCCTGTGGGC AGTATAGGAG GACAGGTTCA AGTGTCCCAA 
CCAGCAGTGA GTTTAGCACA AGCCCCCACT GCATCCTCCC AGCAAGCAGC TCTGGAGAGT 
ACTCAGGGAG TCTCTCAGGT TGCTCCTCCA GAGCCAGTTT CGGTAGCACA GCCACAGCCC 
ACCCAGCCTG CCGCTTTGGT TTCTTCTATA GACAGTGCTC ATTCAGATGT CGCTTCGGGT 
ATGAGCGATG GCAATGAGAA TGTCCCATCA TCTGGTGGAA GGCATGAAGG GAGAACTGCA 
AAACGGCATT ATCGAAAATC TGTAAGAAGC CGCTCTCGTC ATGAAAAGAC TTCACGCCCC 
AAATTGAGAA TTTTGAATGT TTCAAATAAA GGAGACCGGG TAGTAGAATG TCAATTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TAGAGAGAGA GTCATTTGTG 
GATCAAGTGC GGGAAATTAT TGAAAAAGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCTGAGG GTGACCAGGG ATTGGAGAGT CTACAGGGAA AGGATGACTA TGGCTTTTCA 
GGTTCTCAGA AATTGGAGGG AGAATTCAAA CAACCACTTC CTGCATCTTC CATGCCACAG 
CAAATAGGAA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCCGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGCCGATTA CGAGAATCAA AAGTTTTCAC CAGTGAAATG 
TCAGATACAG TTGCCGCCTC TACATCTCAT GGCGCTGGGG TGAACTTGTC CCACTCTGCT 
TCTTCCCTTA GTCTACAGCA GGCCTTTTCT GAACTCAGAC ACGCCCAAGT GACAGAGGGA 
CCCAATACAG CACCTCCCAA CTTCAGTCAT ACAGGACCAA CATTTCCAGT AGTACCTCCT 
TCCATGAGTA GCATTGCTGG AGTCCCAAAG ACAACAGCAG CCGCACCTTC AATATCAGTC 
CCTGTAACTA GCTGCCCTCT TAATGACGTT TCCACGTCAA TAATTCAGCC TGAGATTACA 
GTGCCCACTG AAAAAGGAGT TGCTGGAGTT GCCACCTGCA CAGGTGTAAT AACTTCAAGT 
GGTTTCCCAG TACCCCCTGC ATCTGAATCA CCAGTTCTTT CCAGTGTGGT TTCAAGTATC 
ACAATACCGA CAATTGTCTC AATATCAACA ACATCCCAGT CAGTTCAGGC TCCCACATCT 
GGGAGCGTTG TTTCTAGTGT AGGCACTTTC CCTTCTATAC CAGTTTCAGC AACTTCAGCC 
TCTGCAGTGG GCGGTGCTGC TACCCCAGGG GCTAAGTCTC CACCTCTATT ATCTCAGCAG 
TCAGCAGGCA TTACTACAGG GATAGCCACA TTAACATCAG TTTCTACCAC CATTCCATTT 
CCAAGCACAG CTTCACAGCT ATCTCTTCAG CTTAGCAGCA GCACCTCTGC TCCTACTCTA 
GCTGAAACAG TGGTGGTTAG TGCACACTCA TTAGATAAAA CATCTCATAG CAGTACAGCT 
GGATTGGCTT TGTCCCACTC TGCATCATCT TCCACTTCTT CCCCTGGAGC AGGAGTATCT 
AGTTCTATTT CTCAGTCTGG TGGGGTACAT CCTTTGGTCG TCCCATCAGC TATAGCTTCT 
ACTCCTGTCC TTCCCCAGGC ATCAGTACCT ATTTCTACAC CTTTATTACC CCAAGGACCT 
AGCATCCCAC CATTGGTGCA ACCTGTTGCC AATGTGCCTG CTGTACAGCA GACACTAATT 
CATAGTCAGC CTCAACCAGC TTTGCTTCCC AACCAGCCCC ATACACACTG CCCTGAAATA 
GATGTTGATA CACAACCCAA AGCTCCTGGG ATTGATGACA TAAAGACTTT AGAGGAAAAG 
TTGCGTTCCC TCTTTAGTGA ACACAGCTCT TCTGGCGCTC AACATGCCTC TGTTGCACTG 
GAGACATCAC TAGTAGTAGA GACTAGTGTC ACACCAGGCA TCCCAACCAC TGCTGTTGCA 
CCCAGCAAAC TCATGACTTC AACTACAAGT ACTTGCTTAC CACCAACCAG TTTGCCATTA 
GGAACAGCTG GTTTGTCCGT TACACCACTG GTCACAACTG GGCAAGTTTC TACCCCCGTC 
GGCACTACAT CAGGAGTGAA ACCTGGAACT GCTCCATCAA AGCTACCTTT AACTAAGGCT 
CCAGTGCTGC CAGTGGGTAC TGAACTTCCA GCTGGTACTC CATCCAGTGA GCAGCTGCCA 
CCTATTCCAG GACCTTCACT AACTCAGTCC CAGCAACCTC TGGAAGATCT TGATGCTCAG 
TTAAGAAGAG CACTTAGTCC AGAGACTATT GCGGTGACAT CCGCAGTTGG TCCTGTGTCC 
GTGGTGGCTC CAGCGGCAGT TACAGAAGCA GGAGCACAGC CTCAGAAGGA TGTTTCTCAA 
GTCACAGGAG GTTCTGTCCC AGCAACTAGT TCAGGAACTG GTGTTTTTAA GACGGGGCGA 
TTTCAGGTTT CAGTTGCAAT AGATGATTCC CAAAAAGAGG GTAAAAATAA GTCAGAAGAT 
GCAAAGTCTG TTCATTTTGA ATCCAGCACT TCAGAGTCCT CAGTGCTATC AAGTAGTAGT 
CCAGAGAGTA CCCTGGTGAA GCCAGGCTCT AATGGGGTAA CCATCCATGG TATTTCTTTA 
GATATGCCAG ATAGTGCCCA CAAAACTCCT GCTGCAGAAG CAAAGTCAGA AACTGGGCAA 
CCTACCAAGG TTGGACGTTT TCAGGTGACA ACTACAGCAA ACAAAGTAGG TCGTTTCTCT 
GTATCAAGAA CTGAAGACAG GATCACTGAG GCAAAGAAAG AGGGACCGAC GGCATCTCCT 
CCTTTTATGG ATTTGGAACA AGCTGTTCTT CCTGCTGCGA TACCAAAGAA AGAAAAACCT 
GAACTGTCAG AGCCTTCACA TCTGAACGGG CCATCTTCTG ATTTGGAGGC CGCCTTCCTA 
AGTAGGGATG TAGATGATGG TTCAGGTAGT CCACACTCTC CTCACCAACT GAGCTCAAAG 
AGTCTACCTA TCCAGAATCT AAGTCAAAGC CTTAGTAACT CACTCAACTC CTCTTACATG 
AGTAGTGACA ATGAGTCAGA TATTGAAGAT GAAGACTTAA AGTTAGAGCT GCGACGACTA 
CGAGACAAAC ATCTTAAAGA GATTCAAGAC CTACAGAGTC GCCAGAAGCA TGAAATTGAA 
TCTTTGTATA CTAAACTGGG CAAGGTTCCT CCTGCTGTCA TTATTCCCCC AGCGGCCCCC 
CTTTCAGGAA GAAGAAGGCG ACCCACTAAA AGCAAAGGCA GCAAATCTAG TCGTATCAGT 
TCCTTGGGGA GTAAAAGCCC CCAGCTTTCA GGTAATATGT CTGGTCAGAG TGAAGCTTCA 
GTCTTGCACC CCCAGCAGAC CCTCCACCCT CCCGGCAACA TCCCAGAAAC TGGGCAGAAT 
CAGTTGTTAC AGCCCCTTAA GCCATCTCCC TCCAGTGACA ACCTGTATTC AGCCTTCACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT 
AACTGTGCAT CAGAGCAGGT GACATTCAAA CCTGGTGGCA GGAGGACCCG ATTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT 
AGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACTG ATGACCTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGACGC CATGAATCTT TCAGGCAGGA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCACCTAC CCCCTTTGGC 
ACTCAGTGGA GTGGGACAGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131739.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987225.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	ORo36490
Clone ID Related Accession (Same CDS sequence)	XM_016131739.1
Accession Version	XM_016131739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCAGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC 
ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC TGATGACCTG CACAAGTTGG TAGACAATTG GGCCCGAGAC 
GCCATGAATC TTTCAGGCAG GAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTACGG TTTTCCACCT ACCCCCTTTG GCACTCAGTG GAGTGGGACA 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTACAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGAGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987225.1
CDS	656..7924
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131739.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131739.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCAGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC 
ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC TGATGACCTG CACAAGTTGG TAGACAATTG GGCCCGAGAC 
GCCATGAATC TTTCAGGCAG GAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTACGG TTTTCCACCT ACCCCCTTTG GCACTCAGTG GAGTGGGACA 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTACAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGAGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131740.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987226.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	ORo36491
Clone ID Related Accession (Same CDS sequence)	XM_016131740.1
Accession Version	XM_016131740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC AGAGCAGGTG 
ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA GGACCAGCAG CACAAACACT 
GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACTGA TGACCTGCAC AAGTTGGTAG ACAATTGGGC CCGAGACGCC 
ATGAATCTTT CAGGCAGGAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTACGGTTT TCCACCTACC CCCTTTGGCA CTCAGTGGAG TGGGACAGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTACAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGAGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987226.1
CDS	656..7861
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131740.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131740.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC AGAGCAGGTG 
ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA GGACCAGCAG CACAAACACT 
GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACTGA TGACCTGCAC AAGTTGGTAG ACAATTGGGC CCGAGACGCC 
ATGAATCTTT CAGGCAGGAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTACGGTTT TCCACCTACC CCCTTTGGCA CTCAGTGGAG TGGGACAGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTACAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGAGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131741.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987227.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	ORo36492
Clone ID Related Accession (Same CDS sequence)	XM_016131741.1
Accession Version	XM_016131741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACATTCACTG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCCGAGACGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ACCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987227.1
CDS	656..7852
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131741.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131741.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACATTCACTG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCCGAGACGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ACCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131743.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987229.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	ORo36493
Clone ID Related Accession (Same CDS sequence)	XM_016131743.1
Accession Version	XM_016131743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC AGAGCAGGTG 
ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACTGAT 
GACCTGCACA AGTTGGTAGA CAATTGGGCC CGAGACGCCA TGAATCTTTC AGGCAGGAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCACCTACCC CCTTTGGCAC TCAGTGGAGT GGGACAGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987229.1
CDS	655..7839
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131743.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131743.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC AGAGCAGGTG 
ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACTGAT 
GACCTGCACA AGTTGGTAGA CAATTGGGCC CGAGACGCCA TGAATCTTTC AGGCAGGAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCACCTACCC CCTTTGGCAC TCAGTGGAGT GGGACAGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131744.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987230.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	ORo36494
Clone ID Related Accession (Same CDS sequence)	XM_016131744.1
Accession Version	XM_016131744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC ATTCACTGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
CGAGACGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAC 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987230.1
CDS	655..7809
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131744.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131744.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC ATTCACTGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
CGAGACGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAC 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131745.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987231.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	ORo36495
Clone ID Related Accession (Same CDS sequence)	XM_016131745.1
Accession Version	XM_016131745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987231.1
CDS	654..7724
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131745.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131745.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG CCTGTGTCCG TGGTGGCTCC AGCGGCAGTT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCACAGGAGG TTCTGTCCCA 
GCAACTAGTT CAGGAACTGG TGTTTTTAAG ACGGGGCGAT TTCAGGTTTC AGTTGCAATA 
GATGATTCCC AAAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CCTGGTGAAG 
CCAGGCTCTA ATGGGGTAAC CATCCATGGT ATTTCTTTAG ATATGCCAGA TAGTGCCCAC 
AAAACTCCTG CTGCAGAAGC AAAGTCAGAA ACTGGGCAAC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTAGGT CGTTTCTCTG TATCAAGAAC TGAAGACAGG 
ATCACTGAGG CAAAGAAAGA GGGACCGACG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGCGAT ACCAAAGAAA GAAAAACCTG AACTGTCAGA GCCTTCACAT 
CTGAACGGGC CATCTTCTGA TTTGGAGGCC GCCTTCCTAA GTAGGGATGT AGATGATGGT 
TCAGGTAGTC CACACTCTCC TCACCAACTG AGCTCAAAGA GTCTACCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAACTC ACTCAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATTGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGACAAACA TCTTAAAGAG 
ATTCAAGACC TACAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC 
AAGGTTCCTC CTGCTGTCAT TATTCCCCCA GCGGCCCCCC TTTCAGGAAG AAGAAGGCGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGTATCAGTT CCTTGGGGAG TAAAAGCCCC 
CAGCTTTCAG GTAATATGTC TGGTCAGAGT GAAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CCGGCAACAT CCCAGAAACT GGGCAGAATC AGTTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTGTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131746.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987232.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	ORo36496
Clone ID Related Accession (Same CDS sequence)	XM_016131746.1
Accession Version	XM_016131746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GCTGTGCGAA GTTTAACTGT GCATCAGAGC AGGTGACATT CAAACCTGGT 
GGCAGGAGGA CCCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987232.1
CDS	656..7666
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131746.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131746.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GCTGTGCGAA GTTTAACTGT GCATCAGAGC AGGTGACATT CAAACCTGGT 
GGCAGGAGGA CCCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131747.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987233.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	ORo36497
Clone ID Related Accession (Same CDS sequence)	XM_016131747.1
Accession Version	XM_016131747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCAAGCT CAGCCTCCTG CCATGACGTC CAGCAGGAAG 
GGCACATTCA CTGATGACCT GCACAAGTTG GTAGACAATT GGGCCCGAGA CGCCATGAAT 
CTTTCAGGCA GGAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGG 
AAGTTCTCTG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAACCTGGG TGGCTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCACC TACCCCCTTT GGCACTCAGT GGAGTGGGAC AGGTGGCCCA 
GCACCACAGC CACTTGGCCA GTTCCAACCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGAG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987233.1
CDS	656..7615
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQLPVSQPVPTVQGEPQISVATQPSVVPVHSGAHFLPMGQPLPTSLLPQYPASQIPVSTPHVSTAQTGFSTLPITIAAGINQPLLTLASSATAAAIPGGSTVAPSQLPALLQSVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131747.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131747.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTG TCCAAGGCGA ACCTCAGATC TCAGTTGCGA CACAACCCTC AGTTGTTCCA 
GTGCATTCTG GTGCTCATTT CCTCCCTATG GGACAGCCAC TTCCTACTTC CTTACTCCCT 
CAGTACCCTG CCTCTCAAAT TCCTGTGTCA ACTCCTCATG TGTCCACGGC TCAGACAGGT 
TTCTCAACCC TTCCCATCAC AATTGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC AATCCCAGGG GGATCAACTG TGGCTCCTAG TCAGCTACCA 
GCCCTTCTGC AGTCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAGCT TCTGCAGCCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCCGAGGT TCCACTTCCC 
TCTGGAGATG TTCTATACCA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCAAGCT CAGCCTCCTG CCATGACGTC CAGCAGGAAG 
GGCACATTCA CTGATGACCT GCACAAGTTG GTAGACAATT GGGCCCGAGA CGCCATGAAT 
CTTTCAGGCA GGAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGG 
AAGTTCTCTG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAACCTGGG TGGCTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCACC TACCCCCTTT GGCACTCAGT GGAGTGGGAC AGGTGGCCCA 
GCACCACAGC CACTTGGCCA GTTCCAACCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGAG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131748.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987234.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	ORo36498
Clone ID Related Accession (Same CDS sequence)	XM_016131748.1
Accession Version	XM_016131748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGGGCTTCCC ACCTCGACTG 
CCACCACAAT ACCCAGGAGA TTCAAATATT GCTTCCTCTT CCAGCGTGGC TTCTGTTTCC 
ATTCCTTCTA CAGTCCTATC CCCTCCAATG CCGACAGAAG CATTAGCTAC ACCAGGTTAC 
TTTCCTACAG TGGTGCAGCC TTATATGGAA TCAAATCTTA TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAACCAGCA GTGAGTTTAG CACAAGCCCC CACTGCATCC 
TCCCAGCAAG CAGCTCTGGA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GCTGTGCGAA GTTTAACTGT GCATCAGAGC AGGTGACATT CAAACCTGGT 
GGCAGGAGGA CCCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987234.1
CDS	655..7485
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131748.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131748.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGGGCTTCCC ACCTCGACTG 
CCACCACAAT ACCCAGGAGA TTCAAATATT GCTTCCTCTT CCAGCGTGGC TTCTGTTTCC 
ATTCCTTCTA CAGTCCTATC CCCTCCAATG CCGACAGAAG CATTAGCTAC ACCAGGTTAC 
TTTCCTACAG TGGTGCAGCC TTATATGGAA TCAAATCTTA TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAACCAGCA GTGAGTTTAG CACAAGCCCC CACTGCATCC 
TCCCAGCAAG CAGCTCTGGA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GCTGTGCGAA GTTTAACTGT GCATCAGAGC AGGTGACATT CAAACCTGGT 
GGCAGGAGGA CCCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGTCCTT GCAACCAGCT CTGTAGGACC AGCAGCACAA ACACTGTCGG GGGAACAGTG 
AACAGCCAAG CTGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACTGATGACC TGCACAAGTT GGTAGACAAT TGGGCCCGAG ACGCCATGAA TCTTTCAGGC 
AGGAGAGGAA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGTC AGCTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC 
GGTTTTCCAC CTACCCCCTT TGGCACTCAG TGGAGTGGGA CAGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTAC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGA GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131749.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987235.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	ORo36499
Clone ID Related Accession (Same CDS sequence)	XM_016131749.1
Accession Version	XM_016131749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGG GCTTCCCACC TCGACTGCCA 
CCACAATACC CAGGAGATTC AAATATTGCT TCCTCTTCCA GCGTGGCTTC TGTTTCCATT 
CCTTCTACAG TCCTATCCCC TCCAATGCCG ACAGAAGCAT TAGCTACACC AGGTTACTTT 
CCTACAGTGG TGCAGCCTTA TATGGAATCA AATCTTATGG TTCCTGTGGG CAGTATAGGA 
GGACAGGTTC AAGTGTCCCA ACCAGCAGTG AGTTTAGCAC AAGCCCCCAC TGCATCCTCC 
CAGCAAGCAG CTCTGGAGAG TACTCAGGGA GTCTCTCAGG TTGCTCCTCC AGAGCCAGTT 
TCGGTAGCAC AGCCACAGCC CACCCAGCCT GCCGCTTTGG TTTCTTCTAT AGACAGTGCT 
CATTCAGATG TCGCTTCGGG TATGAGCGAT GGCAATGAGA ATGTCCCATC ATCTGGTGGA 
AGGCATGAAG GGAGAACTGC AAAACGGCAT TATCGAAAAT CTGTAAGAAG CCGCTCTCGT 
CATGAAAAGA CTTCACGCCC CAAATTGAGA ATTTTGAATG TTTCAAATAA AGGAGACCGG 
GTAGTAGAAT GTCAATTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATAGAGAGAG AGTCATTTGT GGATCAAGTG CGGGAAATTA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCTGAG GGTGACCAGG GATTGGAGAG TCTACAGGGA 
AAGGATGACT ATGGCTTTTC AGGTTCTCAG AAATTGGAGG GAGAATTCAA ACAACCACTT 
CCTGCATCTT CCATGCCACA GCAAATAGGA ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCCG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGCCGATT ACGAGAATCA 
AAAGTTTTCA CCAGTGAAAT GTCAGATACA GTTGCCGCCT CTACATCTCA TGGCGCTGGG 
GTGAACTTGT CCCACTCTGC TTCTTCCCTT AGTCTACAGC AGGCCTTTTC TGAACTCAGA 
CACGCCCAAG TGACAGAGGG ACCCAATACA GCACCTCCCA ACTTCAGTCA TACAGGACCA 
ACATTTCCAG TAGTACCTCC TTCCATGAGT AGCATTGCTG GAGTCCCAAA GACAACAGCA 
GCCGCACCTT CAATATCAGT CCCTGTAACT AGCTGCCCTC TTAATGACGT TTCCACGTCA 
ATAATTCAGC CTGAGATTAC AGTGCCCACT GAAAAAGGAG TTGCTGGAGT TGCCACCTGC 
ACAGGTGTAA TAACTTCAAG TGGTTTCCCA GTACCCCCTG CATCTGAATC ACCAGTTCTT 
TCCAGTGTGG TTTCAAGTAT CACAATACCG ACAATTGTCT CAATATCAAC AACATCCCAG 
TCAGTTCAGG CTCCCACATC TGGGAGCGTT GTTTCTAGTG TAGGCACTTT CCCTTCTATA 
CCAGTTTCAG CAACTTCAGC CTCTGCAGTG GGCGGTGCTG CTACCCCAGG GGCTAAGTCT 
CCACCTCTAT TATCTCAGCA GTCAGCAGGC ATTACTACAG GGATAGCCAC ATTAACATCA 
GTTTCTACCA CCATTCCATT TCCAAGCACA GCTTCACAGC TATCTCTTCA GCTTAGCAGC 
AGCACCTCTG CTCCTACTCT AGCTGAAACA GTGGTGGTTA GTGCACACTC ATTAGATAAA 
ACATCTCATA GCAGTACAGC TGGATTGGCT TTGTCCCACT CTGCATCATC TTCCACTTCT 
TCCCCTGGAG CAGGAGTATC TAGTTCTATT TCTCAGTCTG GTGGGGTACA TCCTTTGGTC 
GTCCCATCAG CTATAGCTTC TACTCCTGTC CTTCCCCAGG CATCAGTACC TATTTCTACA 
CCTTTATTAC CCCAAGGACC TAGCATCCCA CCATTGGTGC AACCTGTTGC CAATGTGCCT 
GCTGTACAGC AGACACTAAT TCATAGTCAG CCTCAACCAG CTTTGCTTCC CAACCAGCCC 
CATACACACT GCCCTGAAAT AGATGTTGAT ACACAACCCA AAGCTCCTGG GATTGATGAC 
ATAAAGACTT TAGAGGAAAA GTTGCGTTCC CTCTTTAGTG AACACAGCTC TTCTGGCGCT 
CAACATGCCT CTGTTGCACT GGAGACATCA CTAGTAGTAG AGACTAGTGT CACACCAGGC 
ATCCCAACCA CTGCTGTTGC ACCCAGCAAA CTCATGACTT CAACTACAAG TACTTGCTTA 
CCACCAACCA GTTTGCCATT AGGAACAGCT GGTTTGTCCG TTACACCACT GGTCACAACT 
GGGCAAGTTT CTACCCCCGT CGGCACTACA TCAGGAGTGA AACCTGGAAC TGCTCCATCA 
AAGCTACCTT TAACTAAGGC TCCAGTGCTG CCAGTGGGTA CTGAACTTCC AGCTGGTACT 
CCATCCAGTG AGCAGCTGCC ACCTATTCCA GGACCTTCAC TAACTCAGTC CCAGCAACCT 
CTGGAAGATC TTGATGCTCA GTTAAGAAGA GCACTTAGTC CAGAGACTAT TGCGGTGACA 
TCCGCAGTTG GTCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC 
TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987235.1
CDS	655..7482
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131749.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131749.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGG GCTTCCCACC TCGACTGCCA 
CCACAATACC CAGGAGATTC AAATATTGCT TCCTCTTCCA GCGTGGCTTC TGTTTCCATT 
CCTTCTACAG TCCTATCCCC TCCAATGCCG ACAGAAGCAT TAGCTACACC AGGTTACTTT 
CCTACAGTGG TGCAGCCTTA TATGGAATCA AATCTTATGG TTCCTGTGGG CAGTATAGGA 
GGACAGGTTC AAGTGTCCCA ACCAGCAGTG AGTTTAGCAC AAGCCCCCAC TGCATCCTCC 
CAGCAAGCAG CTCTGGAGAG TACTCAGGGA GTCTCTCAGG TTGCTCCTCC AGAGCCAGTT 
TCGGTAGCAC AGCCACAGCC CACCCAGCCT GCCGCTTTGG TTTCTTCTAT AGACAGTGCT 
CATTCAGATG TCGCTTCGGG TATGAGCGAT GGCAATGAGA ATGTCCCATC ATCTGGTGGA 
AGGCATGAAG GGAGAACTGC AAAACGGCAT TATCGAAAAT CTGTAAGAAG CCGCTCTCGT 
CATGAAAAGA CTTCACGCCC CAAATTGAGA ATTTTGAATG TTTCAAATAA AGGAGACCGG 
GTAGTAGAAT GTCAATTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATAGAGAGAG AGTCATTTGT GGATCAAGTG CGGGAAATTA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCTGAG GGTGACCAGG GATTGGAGAG TCTACAGGGA 
AAGGATGACT ATGGCTTTTC AGGTTCTCAG AAATTGGAGG GAGAATTCAA ACAACCACTT 
CCTGCATCTT CCATGCCACA GCAAATAGGA ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCCG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGCCGATT ACGAGAATCA 
AAAGTTTTCA CCAGTGAAAT GTCAGATACA GTTGCCGCCT CTACATCTCA TGGCGCTGGG 
GTGAACTTGT CCCACTCTGC TTCTTCCCTT AGTCTACAGC AGGCCTTTTC TGAACTCAGA 
CACGCCCAAG TGACAGAGGG ACCCAATACA GCACCTCCCA ACTTCAGTCA TACAGGACCA 
ACATTTCCAG TAGTACCTCC TTCCATGAGT AGCATTGCTG GAGTCCCAAA GACAACAGCA 
GCCGCACCTT CAATATCAGT CCCTGTAACT AGCTGCCCTC TTAATGACGT TTCCACGTCA 
ATAATTCAGC CTGAGATTAC AGTGCCCACT GAAAAAGGAG TTGCTGGAGT TGCCACCTGC 
ACAGGTGTAA TAACTTCAAG TGGTTTCCCA GTACCCCCTG CATCTGAATC ACCAGTTCTT 
TCCAGTGTGG TTTCAAGTAT CACAATACCG ACAATTGTCT CAATATCAAC AACATCCCAG 
TCAGTTCAGG CTCCCACATC TGGGAGCGTT GTTTCTAGTG TAGGCACTTT CCCTTCTATA 
CCAGTTTCAG CAACTTCAGC CTCTGCAGTG GGCGGTGCTG CTACCCCAGG GGCTAAGTCT 
CCACCTCTAT TATCTCAGCA GTCAGCAGGC ATTACTACAG GGATAGCCAC ATTAACATCA 
GTTTCTACCA CCATTCCATT TCCAAGCACA GCTTCACAGC TATCTCTTCA GCTTAGCAGC 
AGCACCTCTG CTCCTACTCT AGCTGAAACA GTGGTGGTTA GTGCACACTC ATTAGATAAA 
ACATCTCATA GCAGTACAGC TGGATTGGCT TTGTCCCACT CTGCATCATC TTCCACTTCT 
TCCCCTGGAG CAGGAGTATC TAGTTCTATT TCTCAGTCTG GTGGGGTACA TCCTTTGGTC 
GTCCCATCAG CTATAGCTTC TACTCCTGTC CTTCCCCAGG CATCAGTACC TATTTCTACA 
CCTTTATTAC CCCAAGGACC TAGCATCCCA CCATTGGTGC AACCTGTTGC CAATGTGCCT 
GCTGTACAGC AGACACTAAT TCATAGTCAG CCTCAACCAG CTTTGCTTCC CAACCAGCCC 
CATACACACT GCCCTGAAAT AGATGTTGAT ACACAACCCA AAGCTCCTGG GATTGATGAC 
ATAAAGACTT TAGAGGAAAA GTTGCGTTCC CTCTTTAGTG AACACAGCTC TTCTGGCGCT 
CAACATGCCT CTGTTGCACT GGAGACATCA CTAGTAGTAG AGACTAGTGT CACACCAGGC 
ATCCCAACCA CTGCTGTTGC ACCCAGCAAA CTCATGACTT CAACTACAAG TACTTGCTTA 
CCACCAACCA GTTTGCCATT AGGAACAGCT GGTTTGTCCG TTACACCACT GGTCACAACT 
GGGCAAGTTT CTACCCCCGT CGGCACTACA TCAGGAGTGA AACCTGGAAC TGCTCCATCA 
AAGCTACCTT TAACTAAGGC TCCAGTGCTG CCAGTGGGTA CTGAACTTCC AGCTGGTACT 
CCATCCAGTG AGCAGCTGCC ACCTATTCCA GGACCTTCAC TAACTCAGTC CCAGCAACCT 
CTGGAAGATC TTGATGCTCA GTTAAGAAGA GCACTTAGTC CAGAGACTAT TGCGGTGACA 
TCCGCAGTTG GTCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC 
TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131750.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987236.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

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CloneID	ORo36500
Clone ID Related Accession (Same CDS sequence)	XM_016131750.1
Accession Version	XM_016131750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGGGCTTCCC ACCTCGACTG 
CCACCACAAT ACCCAGGAGA TTCAAATATT GCTTCCTCTT CCAGCGTGGC TTCTGTTTCC 
ATTCCTTCTA CAGTCCTATC CCCTCCAATG CCGACAGAAG CATTAGCTAC ACCAGGTTAC 
TTTCCTACAG TGGTGCAGCC TTATATGGAA TCAAATCTTA TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAACCAGCA GTGAGTTTAG CACAAGCCCC CACTGCATCC 
TCCCAGCAAG CAGCTCTGGA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CAAGCTCAGC CTCCTGCCAT GACGTCCAGC AGGAAGGGCA CATTCACTGA TGACCTGCAC 
AAGTTGGTAG ACAATTGGGC CCGAGACGCC ATGAATCTTT CAGGCAGGAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA CCTGGGTGGC TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCACCTACC 
CCCTTTGGCA CTCAGTGGAG TGGGACAGGT GGCCCAGCAC CACAGCCACT TGGCCAGTTC 
CAACCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGAGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987236.1
CDS	653..7348
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131750.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131750.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGGGCTTCCC ACCTCGACTG 
CCACCACAAT ACCCAGGAGA TTCAAATATT GCTTCCTCTT CCAGCGTGGC TTCTGTTTCC 
ATTCCTTCTA CAGTCCTATC CCCTCCAATG CCGACAGAAG CATTAGCTAC ACCAGGTTAC 
TTTCCTACAG TGGTGCAGCC TTATATGGAA TCAAATCTTA TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAACCAGCA GTGAGTTTAG CACAAGCCCC CACTGCATCC 
TCCCAGCAAG CAGCTCTGGA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TCCAGAGCCA 
GTTTCGGTAG CACAGCCACA GCCCACCCAG CCTGCCGCTT TGGTTTCTTC TATAGACAGT 
GCTCATTCAG ATGTCGCTTC GGGTATGAGC GATGGCAATG AGAATGTCCC ATCATCTGGT 
GGAAGGCATG AAGGGAGAAC TGCAAAACGG CATTATCGAA AATCTGTAAG AAGCCGCTCT 
CGTCATGAAA AGACTTCACG CCCCAAATTG AGAATTTTGA ATGTTTCAAA TAAAGGAGAC 
CGGGTAGTAG AATGTCAATT AGAGACTCAT AATCGGAAAA TGGTTACATT CAAATTTGAT 
TTAGATGGTG ACAATCCTGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA 
GCAATAGAGA GAGAGTCATT TGTGGATCAA GTGCGGGAAA TTATTGAAAA AGCTGATGAA 
ATGCTCAGTG AGGATGTCAG TGTGGAACCT GAGGGTGACC AGGGATTGGA GAGTCTACAG 
GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AGGGAGAATT CAAACAACCA 
CTTCCTGCAT CTTCCATGCC ACAGCAAATA GGAATTCCTA CCAGTTCTTT AACTCAAGTT 
GTTCATTCTG CCGGAAGACG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA 
TCAAAAGTTT TCACCAGTGA AATGTCAGAT ACAGTTGCCG CCTCTACATC TCATGGCGCT 
GGGGTGAACT TGTCCCACTC TGCTTCTTCC CTTAGTCTAC AGCAGGCCTT TTCTGAACTC 
AGACACGCCC AAGTGACAGA GGGACCCAAT ACAGCACCTC CCAACTTCAG TCATACAGGA 
CCAACATTTC CAGTAGTACC TCCTTCCATG AGTAGCATTG CTGGAGTCCC AAAGACAACA 
GCAGCCGCAC CTTCAATATC AGTCCCTGTA ACTAGCTGCC CTCTTAATGA CGTTTCCACG 
TCAATAATTC AGCCTGAGAT TACAGTGCCC ACTGAAAAAG GAGTTGCTGG AGTTGCCACC 
TGCACAGGTG TAATAACTTC AAGTGGTTTC CCAGTACCCC CTGCATCTGA ATCACCAGTT 
CTTTCCAGTG TGGTTTCAAG TATCACAATA CCGACAATTG TCTCAATATC AACAACATCC 
CAGTCAGTTC AGGCTCCCAC ATCTGGGAGC GTTGTTTCTA GTGTAGGCAC TTTCCCTTCT 
ATACCAGTTT CAGCAACTTC AGCCTCTGCA GTGGGCGGTG CTGCTACCCC AGGGGCTAAG 
TCTCCACCTC TATTATCTCA GCAGTCAGCA GGCATTACTA CAGGGATAGC CACATTAACA 
TCAGTTTCTA CCACCATTCC ATTTCCAAGC ACAGCTTCAC AGCTATCTCT TCAGCTTAGC 
AGCAGCACCT CTGCTCCTAC TCTAGCTGAA ACAGTGGTGG TTAGTGCACA CTCATTAGAT 
AAAACATCTC ATAGCAGTAC AGCTGGATTG GCTTTGTCCC ACTCTGCATC ATCTTCCACT 
TCTTCCCCTG GAGCAGGAGT ATCTAGTTCT ATTTCTCAGT CTGGTGGGGT ACATCCTTTG 
GTCGTCCCAT CAGCTATAGC TTCTACTCCT GTCCTTCCCC AGGCATCAGT ACCTATTTCT 
ACACCTTTAT TACCCCAAGG ACCTAGCATC CCACCATTGG TGCAACCTGT TGCCAATGTG 
CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG 
CCCCATACAC ACTGCCCTGA AATAGATGTT GATACACAAC CCAAAGCTCC TGGGATTGAT 
GACATAAAGA CTTTAGAGGA AAAGTTGCGT TCCCTCTTTA GTGAACACAG CTCTTCTGGC 
GCTCAACATG CCTCTGTTGC ACTGGAGACA TCACTAGTAG TAGAGACTAG TGTCACACCA 
GGCATCCCAA CCACTGCTGT TGCACCCAGC AAACTCATGA CTTCAACTAC AAGTACTTGC 
TTACCACCAA CCAGTTTGCC ATTAGGAACA GCTGGTTTGT CCGTTACACC ACTGGTCACA 
ACTGGGCAAG TTTCTACCCC CGTCGGCACT ACATCAGGAG TGAAACCTGG AACTGCTCCA 
TCAAAGCTAC CTTTAACTAA GGCTCCAGTG CTGCCAGTGG GTACTGAACT TCCAGCTGGT 
ACTCCATCCA GTGAGCAGCT GCCACCTATT CCAGGACCTT CACTAACTCA GTCCCAGCAA 
CCTCTGGAAG ATCTTGATGC TCAGTTAAGA AGAGCACTTA GTCCAGAGAC TATTGCGGTG 
ACATCCGCAG TTGGTCCTGT GTCCGTGGTG GCTCCAGCGG CAGTTACAGA AGCAGGAGCA 
CAGCCTCAGA AGGATGTTTC TCAAGTCACA GGAGGTTCTG TCCCAGCAAC TAGTTCAGGA 
ACTGGTGTTT TTAAGACGGG GCGATTTCAG GTTTCAGTTG CAATAGATGA TTCCCAAAAA 
GAGGGTAAAA ATAAGTCAGA AGATGCAAAG TCTGTTCATT TTGAATCCAG CACTTCAGAG 
TCCTCAGTGC TATCAAGTAG TAGTCCAGAG AGTACCCTGG TGAAGCCAGG CTCTAATGGG 
GTAACCATCC ATGGTATTTC TTTAGATATG CCAGATAGTG CCCACAAAAC TCCTGCTGCA 
GAAGCAAAGT CAGAAACTGG GCAACCTACC AAGGTTGGAC GTTTTCAGGT GACAACTACA 
GCAAACAAAG TAGGTCGTTT CTCTGTATCA AGAACTGAAG ACAGGATCAC TGAGGCAAAG 
AAAGAGGGAC CGACGGCATC TCCTCCTTTT ATGGATTTGG AACAAGCTGT TCTTCCTGCT 
GCGATACCAA AGAAAGAAAA ACCTGAACTG TCAGAGCCTT CACATCTGAA CGGGCCATCT 
TCTGATTTGG AGGCCGCCTT CCTAAGTAGG GATGTAGATG ATGGTTCAGG TAGTCCACAC 
TCTCCTCACC AACTGAGCTC AAAGAGTCTA CCTATCCAGA ATCTAAGTCA AAGCCTTAGT 
AACTCACTCA ACTCCTCTTA CATGAGTAGT GACAATGAGT CAGATATTGA AGATGAAGAC 
TTAAAGTTAG AGCTGCGACG ACTACGAGAC AAACATCTTA AAGAGATTCA AGACCTACAG 
AGTCGCCAGA AGCATGAAAT TGAATCTTTG TATACTAAAC TGGGCAAGGT TCCTCCTGCT 
GTCATTATTC CCCCAGCGGC CCCCCTTTCA GGAAGAAGAA GGCGACCCAC TAAAAGCAAA 
GGCAGCAAAT CTAGTCGTAT CAGTTCCTTG GGGAGTAAAA GCCCCCAGCT TTCAGGTAAT 
ATGTCTGGTC AGAGTGAAGC TTCAGTCTTG CACCCCCAGC AGACCCTCCA CCCTCCCGGC 
AACATCCCAG AAACTGGGCA GAATCAGTTG TTACAGCCCC TTAAGCCATC TCCCTCCAGT 
GACAACCTGT ATTCAGCCTT CACCAGTGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCT 
CCAGGTCAAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CAAGCTCAGC CTCCTGCCAT GACGTCCAGC AGGAAGGGCA CATTCACTGA TGACCTGCAC 
AAGTTGGTAG ACAATTGGGC CCGAGACGCC ATGAATCTTT CAGGCAGGAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA CCTGGGTGGC TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCACCTACC 
CCCTTTGGCA CTCAGTGGAG TGGGACAGGT GGCCCAGCAC CACAGCCACT TGGCCAGTTC 
CAACCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGAGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131751.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987237.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

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CloneID	ORo36501
Clone ID Related Accession (Same CDS sequence)	XM_016131751.1
Accession Version	XM_016131751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGA CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGGAGGTTCT 
GTCCCAGCAA CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAAAA AGAGGGTAAA AATAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG GCTCTAATGG GGTAACCATC CATGGTATTT CTTTAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCTGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGATCA CTGAGGCAAA GAAAGAGGGA CCGACGGCAT CTCCTCCTTT TATGGATTTG 
GAACAAGCTG TTCTTCCTGC TGCGATACCA AAGAAAGAAA AACCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGGCCATC TTCTGATTTG GAGGCCGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCTCCTCAC CAACTGAGCT CAAAGAGTCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAGATTC AAGACCTACA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT GCACCCCCAG 
CAGACCCTCC ACCCTCCCGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCAGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACATT CACTGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCCGA 
GACGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAACCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
AGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987237.1
CDS	654..7205
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131751.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131751.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGA CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGGAGGTTCT 
GTCCCAGCAA CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAAAA AGAGGGTAAA AATAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG GCTCTAATGG GGTAACCATC CATGGTATTT CTTTAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCTGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGATCA CTGAGGCAAA GAAAGAGGGA CCGACGGCAT CTCCTCCTTT TATGGATTTG 
GAACAAGCTG TTCTTCCTGC TGCGATACCA AAGAAAGAAA AACCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGGCCATC TTCTGATTTG GAGGCCGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCTCCTCAC CAACTGAGCT CAAAGAGTCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAGATTC AAGACCTACA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT GCACCCCCAG 
CAGACCCTCC ACCCTCCCGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCAGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACATT CACTGATGAC CTGCACAAGT TGGTAGACAA TTGGGCCCGA 
GACGCCATGA ATCTTTCAGG CAGGAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAACCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA CCTACCCCCT TTGGCACTCA GTGGAGTGGG 
ACAGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
AGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131752.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987238.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

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CloneID	ORo36502
Clone ID Related Accession (Same CDS sequence)	XM_016131752.1
Accession Version	XM_016131752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC 
TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987238.1
CDS	653..7120
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131752.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131752.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG TACGCCCTGC TTGGCTCTTT GGAAGATGGT GAAAAAAGTC 
TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAACA CTGTCGGGGG AACAGTGAAC 
AGCCAAGCTG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACT 
GATGACCTGC ACAAGTTGGT AGACAATTGG GCCCGAGACG CCATGAATCT TTCAGGCAGG 
AGAGGAAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGTCAGC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTACGGT 
TTTCCACCTA CCCCCTTTGG CACTCAGTGG AGTGGGACAG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTACAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGTTCC AACCTGAGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131753.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987239.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

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CloneID	ORo36503
Clone ID Related Accession (Same CDS sequence)	XM_016131753.1
Accession Version	XM_016131753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT 
AGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACTG ATGACCTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGACGC CATGAATCTT TCAGGCAGGA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCACCTAC CCCCTTTGGC 
ACTCAGTGGA GTGGGACAGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987239.1
CDS	652..7098
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131753.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131753.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGCT GTGCGAAGTT TAACTGTGCA TCAGAGCAGG TGACATTCAA ACCTGGTGGC 
AGGAGGACCC GATTTCTGAG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT 
AGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACTG ATGACCTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGACGC CATGAATCTT TCAGGCAGGA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCACCTAC CCCCTTTGGC 
ACTCAGTGGA GTGGGACAGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131754.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987240.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

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CloneID	ORo36504
Clone ID Related Accession (Same CDS sequence)	XM_016131754.1
Accession Version	XM_016131754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGA CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGGAGGTTCT 
GTCCCAGCAA CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAAAA AGAGGGTAAA AATAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG GCTCTAATGG GGTAACCATC CATGGTATTT CTTTAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCTGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGATCA CTGAGGCAAA GAAAGAGGGA CCGACGGCAT CTCCTCCTTT TATGGATTTG 
GAACAAGCTG TTCTTCCTGC TGCGATACCA AAGAAAGAAA AACCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGGCCATC TTCTGATTTG GAGGCCGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCTCCTCAC CAACTGAGCT CAAAGAGTCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAGATTC AAGACCTACA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT GCACCCCCAG 
CAGACCCTCC ACCCTCCCGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACATTCACTG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCCGAGACGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ACCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987240.1
CDS	652..7068
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131754.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131754.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTAAG AAGAGCACTT 
AGTCCAGAGA CTATTGCGGT GACATCCGCA GTTGGTCCTG TGTCCGTGGT GGCTCCAGCG 
GCAGTTACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAAGTCAC AGGAGGTTCT 
GTCCCAGCAA CTAGTTCAGG AACTGGTGTT TTTAAGACGG GGCGATTTCA GGTTTCAGTT 
GCAATAGATG ATTCCCAAAA AGAGGGTAAA AATAAGTCAG AAGATGCAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA GAGTACCCTG 
GTGAAGCCAG GCTCTAATGG GGTAACCATC CATGGTATTT CTTTAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCTGC AGAAGCAAAG TCAGAAACTG GGCAACCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTATC AAGAACTGAA 
GACAGGATCA CTGAGGCAAA GAAAGAGGGA CCGACGGCAT CTCCTCCTTT TATGGATTTG 
GAACAAGCTG TTCTTCCTGC TGCGATACCA AAGAAAGAAA AACCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGGCCATC TTCTGATTTG GAGGCCGCCT TCCTAAGTAG GGATGTAGAT 
GATGGTTCAG GTAGTCCACA CTCTCCTCAC CAACTGAGCT CAAAGAGTCT ACCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAACTCACTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA CTTAAAGTTA GAGCTGCGAC GACTACGAGA CAAACATCTT 
AAAGAGATTC AAGACCTACA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACTAAA 
CTGGGCAAGG TTCCTCCTGC TGTCATTATT CCCCCAGCGG CCCCCCTTTC AGGAAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGTCGTA TCAGTTCCTT GGGGAGTAAA 
AGCCCCCAGC TTTCAGGTAA TATGTCTGGT CAGAGTGAAG CTTCAGTCTT GCACCCCCAG 
CAGACCCTCC ACCCTCCCGG CAACATCCCA GAAACTGGGC AGAATCAGTT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTG TATTCAGCCT TCACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGTCAA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCAAGCTCAG CCTCCTGCCA TGACGTCCAG CAGGAAGGGC 
ACATTCACTG ATGACCTGCA CAAGTTGGTA GACAATTGGG CCCGAGACGC CATGAATCTT 
TCAGGCAGGA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG 
TTCTCTGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ACCTGGGTGG CTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCACCTAC CCCCTTTGGC ACTCAGTGGA GTGGGACAGG TGGCCCAGCA 
CCACAGCCAC TTGGCCAGTT CCAACCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGAGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131755.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987241.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

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CloneID	ORo36505
Clone ID Related Accession (Same CDS sequence)	XM_016131755.1
Accession Version	XM_016131755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCGCTTTG 
GTTTCTTCTA TAGACAGTGC TCATTCAGAT GTCGCTTCGG GTATGAGCGA TGGCAATGAG 
AATGTCCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GCCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAATTAG AGACTCATAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCTGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCATTTG TGGATCAAGT GCGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCTGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAG 
GGAGAATTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG AATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCC GGAAGACGGT TTATAGTGAG TCCTGTGCCA 
GAAAGCCGAT TACGAGAATC AAAAGTTTTC ACCAGTGAAA TGTCAGATAC AGTTGCCGCC 
TCTACATCTC ATGGCGCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTCAG ACACGCCCAA GTGACAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATACAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAGTCCCAA AGACAACAGC AGCCGCACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACG TTTCCACGTC AATAATTCAG CCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCCCCT 
GCATCTGAAT CACCAGTTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC GACAATTGTC 
TCAATATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
GTAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCGGTGCT 
GCTACCCCAG GGGCTAAGTC TCCACCTCTA TTATCTCAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCATTCCAT TTCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CATTAGATAA AACATCTCAT AGCAGTACAG CTGGATTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAGTCT 
GGTGGGGTAC ATCCTTTGGT CGTCCCATCA GCTATAGCTT CTACTCCTGT CCTTCCCCAG 
GCATCAGTAC CTATTTCTAC ACCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC CCAACCAGCC CCATACACAC TGCCCTGAAA TAGATGTTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT TTAGAGGAAA AGTTGCGTTC CCTCTTTAGT 
GAACACAGCT CTTCTGGCGC TCAACATGCC TCTGTTGCAC TGGAGACATC ACTAGTAGTA 
GAGACTAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
GTTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCCG TCGGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGG AGGTTCTGTC 
CCAGCAACTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAAAAAGA GGGTAAAAAT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGGCT CTAATGGGGT AACCATCCAT GGTATTTCTT TAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCTGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGATCACTG AGGCAAAGAA AGAGGGACCG ACGGCATCTC CTCCTTTTAT GGATTTGGAA 
CAAGCTGTTC TTCCTGCTGC GATACCAAAG AAAGAAAAAC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GGCCATCTTC TGATTTGGAG GCCGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC TCCTCACCAA CTGAGCTCAA AGAGTCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAGATTCAAG ACCTACAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTGCA CCCCCAGCAG 
ACCCTCCACC CTCCCGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAACAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACA 
TTCACTGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCC GAGACGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TACGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGAGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987241.1
CDS	651..7064
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131755.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131755.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCATGGAAT ACAACAGACA GCCCCTCCTC AACAGACAGT GCAGTATTCA 
CTTCCACAGA CATCAACCTC CAGTGAGGCC ACTACTGCAC AGCCAGTGAG TCAGCCTCAA 
GCTCCACAGG TCTTGCCTCA AGCATCAGCT GGAAAACAGA GTACTCAGGG AGTCTCTCAG 
GTTGCTCCTC CAGAGCCAGT TTCGGTAGCA CAGCCACAGC CCACCCAGCC TGCCGCTTTG 
GTTTCTTCTA TAGACAGTGC TCATTCAGAT GTCGCTTCGG GTATGAGCGA TGGCAATGAG 
AATGTCCCAT CATCTGGTGG AAGGCATGAA GGGAGAACTG CAAAACGGCA TTATCGAAAA 
TCTGTAAGAA GCCGCTCTCG TCATGAAAAG ACTTCACGCC CCAAATTGAG AATTTTGAAT 
GTTTCAAATA AAGGAGACCG GGTAGTAGAA TGTCAATTAG AGACTCATAA TCGGAAAATG 
GTTACATTCA AATTTGATTT AGATGGTGAC AATCCTGAGG AGATAGCAAC AATTATGGTG 
AACAATGACT TTATTCTAGC AATAGAGAGA GAGTCATTTG TGGATCAAGT GCGGGAAATT 
ATTGAAAAAG CTGATGAAAT GCTCAGTGAG GATGTCAGTG TGGAACCTGA GGGTGACCAG 
GGATTGGAGA GTCTACAGGG AAAGGATGAC TATGGCTTTT CAGGTTCTCA GAAATTGGAG 
GGAGAATTCA AACAACCACT TCCTGCATCT TCCATGCCAC AGCAAATAGG AATTCCTACC 
AGTTCTTTAA CTCAAGTTGT TCATTCTGCC GGAAGACGGT TTATAGTGAG TCCTGTGCCA 
GAAAGCCGAT TACGAGAATC AAAAGTTTTC ACCAGTGAAA TGTCAGATAC AGTTGCCGCC 
TCTACATCTC ATGGCGCTGG GGTGAACTTG TCCCACTCTG CTTCTTCCCT TAGTCTACAG 
CAGGCCTTTT CTGAACTCAG ACACGCCCAA GTGACAGAGG GACCCAATAC AGCACCTCCC 
AACTTCAGTC ATACAGGACC AACATTTCCA GTAGTACCTC CTTCCATGAG TAGCATTGCT 
GGAGTCCCAA AGACAACAGC AGCCGCACCT TCAATATCAG TCCCTGTAAC TAGCTGCCCT 
CTTAATGACG TTTCCACGTC AATAATTCAG CCTGAGATTA CAGTGCCCAC TGAAAAAGGA 
GTTGCTGGAG TTGCCACCTG CACAGGTGTA ATAACTTCAA GTGGTTTCCC AGTACCCCCT 
GCATCTGAAT CACCAGTTCT TTCCAGTGTG GTTTCAAGTA TCACAATACC GACAATTGTC 
TCAATATCAA CAACATCCCA GTCAGTTCAG GCTCCCACAT CTGGGAGCGT TGTTTCTAGT 
GTAGGCACTT TCCCTTCTAT ACCAGTTTCA GCAACTTCAG CCTCTGCAGT GGGCGGTGCT 
GCTACCCCAG GGGCTAAGTC TCCACCTCTA TTATCTCAGC AGTCAGCAGG CATTACTACA 
GGGATAGCCA CATTAACATC AGTTTCTACC ACCATTCCAT TTCCAAGCAC AGCTTCACAG 
CTATCTCTTC AGCTTAGCAG CAGCACCTCT GCTCCTACTC TAGCTGAAAC AGTGGTGGTT 
AGTGCACACT CATTAGATAA AACATCTCAT AGCAGTACAG CTGGATTGGC TTTGTCCCAC 
TCTGCATCAT CTTCCACTTC TTCCCCTGGA GCAGGAGTAT CTAGTTCTAT TTCTCAGTCT 
GGTGGGGTAC ATCCTTTGGT CGTCCCATCA GCTATAGCTT CTACTCCTGT CCTTCCCCAG 
GCATCAGTAC CTATTTCTAC ACCTTTATTA CCCCAAGGAC CTAGCATCCC ACCATTGGTG 
CAACCTGTTG CCAATGTGCC TGCTGTACAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTGCTTC CCAACCAGCC CCATACACAC TGCCCTGAAA TAGATGTTGA TACACAACCC 
AAAGCTCCTG GGATTGATGA CATAAAGACT TTAGAGGAAA AGTTGCGTTC CCTCTTTAGT 
GAACACAGCT CTTCTGGCGC TCAACATGCC TCTGTTGCAC TGGAGACATC ACTAGTAGTA 
GAGACTAGTG TCACACCAGG CATCCCAACC ACTGCTGTTG CACCCAGCAA ACTCATGACT 
TCAACTACAA GTACTTGCTT ACCACCAACC AGTTTGCCAT TAGGAACAGC TGGTTTGTCC 
GTTACACCAC TGGTCACAAC TGGGCAAGTT TCTACCCCCG TCGGCACTAC ATCAGGAGTG 
AAACCTGGAA CTGCTCCATC AAAGCTACCT TTAACTAAGG CTCCAGTGCT GCCAGTGGGT 
ACTGAACTTC CAGCTGGTAC TCCATCCAGT GAGCAGCTGC CACCTATTCC AGGACCTTCA 
CTAACTCAGT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTAAGAAG AGCACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCCGCAGTT GGTCCTGTGT CCGTGGTGGC TCCAGCGGCA 
GTTACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AAGTCACAGG AGGTTCTGTC 
CCAGCAACTA GTTCAGGAAC TGGTGTTTTT AAGACGGGGC GATTTCAGGT TTCAGTTGCA 
ATAGATGATT CCCAAAAAGA GGGTAAAAAT AAGTCAGAAG ATGCAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAGAG TACCCTGGTG 
AAGCCAGGCT CTAATGGGGT AACCATCCAT GGTATTTCTT TAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCTGCAGA AGCAAAGTCA GAAACTGGGC AACCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTATCAAG AACTGAAGAC 
AGGATCACTG AGGCAAAGAA AGAGGGACCG ACGGCATCTC CTCCTTTTAT GGATTTGGAA 
CAAGCTGTTC TTCCTGCTGC GATACCAAAG AAAGAAAAAC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GGCCATCTTC TGATTTGGAG GCCGCCTTCC TAAGTAGGGA TGTAGATGAT 
GGTTCAGGTA GTCCACACTC TCCTCACCAA CTGAGCTCAA AGAGTCTACC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA CTCACTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGACTT AAAGTTAGAG CTGCGACGAC TACGAGACAA ACATCTTAAA 
GAGATTCAAG ACCTACAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG 
GGCAAGGTTC CTCCTGCTGT CATTATTCCC CCAGCGGCCC CCCTTTCAGG AAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAATCT AGTCGTATCA GTTCCTTGGG GAGTAAAAGC 
CCCCAGCTTT CAGGTAATAT GTCTGGTCAG AGTGAAGCTT CAGTCTTGCA CCCCCAGCAG 
ACCCTCCACC CTCCCGGCAA CATCCCAGAA ACTGGGCAGA ATCAGTTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTGTAT TCAGCCTTCA CCAGTGATGG TGCCATTTCA 
GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAACAGCC AAGCTGCCCA AGCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGCACA 
TTCACTGATG ACCTGCACAA GTTGGTAGAC AATTGGGCCC GAGACGCCAT GAATCTTTCA 
GGCAGGAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC 
TCTGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TACGGTTTTC CACCTACCCC CTTTGGCACT CAGTGGAGTG GGACAGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGAGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131756.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987242.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

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CloneID	ORo36506
Clone ID Related Accession (Same CDS sequence)	XM_016131756.1
Accession Version	XM_016131756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACTGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCCG AGACGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GAGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987242.1
CDS	651..6983
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQSTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131756.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131756.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGGC ACAGGGGCAG 
AGCCAGGGTC AGCCATCCTC AAGTAGTGTA ACAGGGGTTT CATCTTCCCA ATCCATACAA 
CATTCTCAGC AGCAGCATGG AATACAACAG ACAGCCCCTC CTCAACAGAC AGTGCAGTAT 
TCACTTCCAC AGACATCAAC CTCCAGTGAG GCCACTACTG CACAGCCAGT GAGTCAGCCT 
CAAGCTCCAC AGGTCTTGCC TCAAGCATCA GCTGGAAAAC AGAGTACTCA GGGAGTCTCT 
CAGGTTGCTC CTCCAGAGCC AGTTTCGGTA GCACAGCCAC AGCCCACCCA GCCTGCCGCT 
TTGGTTTCTT CTATAGACAG TGCTCATTCA GATGTCGCTT CGGGTATGAG CGATGGCAAT 
GAGAATGTCC CATCATCTGG TGGAAGGCAT GAAGGGAGAA CTGCAAAACG GCATTATCGA 
AAATCTGTAA GAAGCCGCTC TCGTCATGAA AAGACTTCAC GCCCCAAATT GAGAATTTTG 
AATGTTTCAA ATAAAGGAGA CCGGGTAGTA GAATGTCAAT TAGAGACTCA TAATCGGAAA 
ATGGTTACAT TCAAATTTGA TTTAGATGGT GACAATCCTG AGGAGATAGC AACAATTATG 
GTGAACAATG ACTTTATTCT AGCAATAGAG AGAGAGTCAT TTGTGGATCA AGTGCGGGAA 
ATTATTGAAA AAGCTGATGA AATGCTCAGT GAGGATGTCA GTGTGGAACC TGAGGGTGAC 
CAGGGATTGG AGAGTCTACA GGGAAAGGAT GACTATGGCT TTTCAGGTTC TCAGAAATTG 
GAGGGAGAAT TCAAACAACC ACTTCCTGCA TCTTCCATGC CACAGCAAAT AGGAATTCCT 
ACCAGTTCTT TAACTCAAGT TGTTCATTCT GCCGGAAGAC GGTTTATAGT GAGTCCTGTG 
CCAGAAAGCC GATTACGAGA ATCAAAAGTT TTCACCAGTG AAATGTCAGA TACAGTTGCC 
GCCTCTACAT CTCATGGCGC TGGGGTGAAC TTGTCCCACT CTGCTTCTTC CCTTAGTCTA 
CAGCAGGCCT TTTCTGAACT CAGACACGCC CAAGTGACAG AGGGACCCAA TACAGCACCT 
CCCAACTTCA GTCATACAGG ACCAACATTT CCAGTAGTAC CTCCTTCCAT GAGTAGCATT 
GCTGGAGTCC CAAAGACAAC AGCAGCCGCA CCTTCAATAT CAGTCCCTGT AACTAGCTGC 
CCTCTTAATG ACGTTTCCAC GTCAATAATT CAGCCTGAGA TTACAGTGCC CACTGAAAAA 
GGAGTTGCTG GAGTTGCCAC CTGCACAGGT GTAATAACTT CAAGTGGTTT CCCAGTACCC 
CCTGCATCTG AATCACCAGT TCTTTCCAGT GTGGTTTCAA GTATCACAAT ACCGACAATT 
GTCTCAATAT CAACAACATC CCAGTCAGTT CAGGCTCCCA CATCTGGGAG CGTTGTTTCT 
AGTGTAGGCA CTTTCCCTTC TATACCAGTT TCAGCAACTT CAGCCTCTGC AGTGGGCGGT 
GCTGCTACCC CAGGGGCTAA GTCTCCACCT CTATTATCTC AGCAGTCAGC AGGCATTACT 
ACAGGGATAG CCACATTAAC ATCAGTTTCT ACCACCATTC CATTTCCAAG CACAGCTTCA 
CAGCTATCTC TTCAGCTTAG CAGCAGCACC TCTGCTCCTA CTCTAGCTGA AACAGTGGTG 
GTTAGTGCAC ACTCATTAGA TAAAACATCT CATAGCAGTA CAGCTGGATT GGCTTTGTCC 
CACTCTGCAT CATCTTCCAC TTCTTCCCCT GGAGCAGGAG TATCTAGTTC TATTTCTCAG 
TCTGGTGGGG TACATCCTTT GGTCGTCCCA TCAGCTATAG CTTCTACTCC TGTCCTTCCC 
CAGGCATCAG TACCTATTTC TACACCTTTA TTACCCCAAG GACCTAGCAT CCCACCATTG 
GTGCAACCTG TTGCCAATGT GCCTGCTGTA CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTGC TTCCCAACCA GCCCCATACA CACTGCCCTG AAATAGATGT TGATACACAA 
CCCAAAGCTC CTGGGATTGA TGACATAAAG ACTTTAGAGG AAAAGTTGCG TTCCCTCTTT 
AGTGAACACA GCTCTTCTGG CGCTCAACAT GCCTCTGTTG CACTGGAGAC ATCACTAGTA 
GTAGAGACTA GTGTCACACC AGGCATCCCA ACCACTGCTG TTGCACCCAG CAAACTCATG 
ACTTCAACTA CAAGTACTTG CTTACCACCA ACCAGTTTGC CATTAGGAAC AGCTGGTTTG 
TCCGTTACAC CACTGGTCAC AACTGGGCAA GTTTCTACCC CCGTCGGCAC TACATCAGGA 
GTGAAACCTG GAACTGCTCC ATCAAAGCTA CCTTTAACTA AGGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCATCC AGTGAGCAGC TGCCACCTAT TCCAGGACCT 
TCACTAACTC AGCCTGTGTC CGTGGTGGCT CCAGCGGCAG TTACAGAAGC AGGAGCACAG 
CCTCAGAAGG ATGTTTCTCA AGTCACAGGA GGTTCTGTCC CAGCAACTAG TTCAGGAACT 
GGTGTTTTTA AGACGGGGCG ATTTCAGGTT TCAGTTGCAA TAGATGATTC CCAAAAAGAG 
GGTAAAAATA AGTCAGAAGA TGCAAAGTCT GTTCATTTTG AATCCAGCAC TTCAGAGTCC 
TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT ACCCTGGTGA AGCCAGGCTC TAATGGGGTA 
ACCATCCATG GTATTTCTTT AGATATGCCA GATAGTGCCC ACAAAACTCC TGCTGCAGAA 
GCAAAGTCAG AAACTGGGCA ACCTACCAAG GTTGGACGTT TTCAGGTGAC AACTACAGCA 
AACAAAGTAG GTCGTTTCTC TGTATCAAGA ACTGAAGACA GGATCACTGA GGCAAAGAAA 
GAGGGACCGA CGGCATCTCC TCCTTTTATG GATTTGGAAC AAGCTGTTCT TCCTGCTGCG 
ATACCAAAGA AAGAAAAACC TGAACTGTCA GAGCCTTCAC ATCTGAACGG GCCATCTTCT 
GATTTGGAGG CCGCCTTCCT AAGTAGGGAT GTAGATGATG GTTCAGGTAG TCCACACTCT 
CCTCACCAAC TGAGCTCAAA GAGTCTACCT ATCCAGAATC TAAGTCAAAG CCTTAGTAAC 
TCACTCAACT CCTCTTACAT GAGTAGTGAC AATGAGTCAG ATATTGAAGA TGAAGACTTA 
AAGTTAGAGC TGCGACGACT ACGAGACAAA CATCTTAAAG AGATTCAAGA CCTACAGAGT 
CGCCAGAAGC ATGAAATTGA ATCTTTGTAT ACTAAACTGG GCAAGGTTCC TCCTGCTGTC 
ATTATTCCCC CAGCGGCCCC CCTTTCAGGA AGAAGAAGGC GACCCACTAA AAGCAAAGGC 
AGCAAATCTA GTCGTATCAG TTCCTTGGGG AGTAAAAGCC CCCAGCTTTC AGGTAATATG 
TCTGGTCAGA GTGAAGCTTC AGTCTTGCAC CCCCAGCAGA CCCTCCACCC TCCCGGCAAC 
ATCCCAGAAA CTGGGCAGAA TCAGTTGTTA CAGCCCCTTA AGCCATCTCC CTCCAGTGAC 
AACCTGTATT CAGCCTTCAC CAGTGATGGT GCCATTTCAG TACCAAGCCT TTCTGCTCCA 
GGTCAAGGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACTGATGA CCTGCACAAG 
TTGGTAGACA ATTGGGCCCG AGACGCCATG AATCTTTCAG GCAGGAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC ACCTACCCCC 
TTTGGCACTC AGTGGAGTGG GACAGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GAGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_016131759.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015987245.1 Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

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CloneID	ORo36508
Clone ID Related Accession (Same CDS sequence)	XM_016131759.1
Accession Version	XM_016131759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	serine/threonine-protein kinase WNK1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015495229.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACTGAAGGT AGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATTCTA TATGTGAACC AGCTCAAGTG TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTGCAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACCTC GAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCTGCTGT GTTAACTCAT 
AACAATGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTATCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACT ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCATCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA GCCATCCTCA 
AGTAGTGTAA CAGGGGTTTC ATCTTCCCAA TCCATACAAC ATTCTCAGCA GCAGCATGGA 
ATACAACAGA CAGCCCCTCC TCAACAGACA GTGCAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AAGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC ATTCACTGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
CGAGACGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAC 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015987245.1
CDS	661..8595
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGTAGTDAGTGRTEEYRRRRHTMDKDSRGAAATTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSVAAVVPQSTPPELQREETLTATAAAQVAQQPPTASTAVSGEPAAAGPVATTAPSSTSKDRQVSQPNHVGSKEEPPSARSGSGGGSSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSLKQQVEQQPSASQAGIKQLSSASTGIPTASATSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTFPGHTASVVQAQSQPHGVYPPSSMPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPLKVAISQRRKSTSFLEAQTHHFQPLLRTVGQNLLPPGGSPTNWTPEAVVMLGSTTSRISGEPCDIQVNSICEPAQVYSDYRPGLVLPEEAHYFIPQEAVYLAGVQYQAQVAEQYEGIPYNSPVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLSPGSPLSSISAPISTDATCLKFHPVFVPHSAPAVLTHNNENRSNCVFEFHVHTPSSSSGEGGGVLSQRVYRNRQVAVDLNQEELPPQSVGVHGRLQPVTEEQHNYHVPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAPASFGSDVSLPFIRLPQTVLQESPLFFCFPQGTTSQQILSASFSSGGSALHPQAQGQSQGQPSSSSVTGVSSSQSIQHSQQQHGIQQTAPPQQTVQYSLPQTSTSSEATTAQPVSQPQAPQVLPQASAGKQGFPPRLPPQYPGDSNIASSSSVASVSIPSTVLSPPMPTEALATPGYFPTVVQPYMESNLMVPVGSIGGQVQVSQPAVSLAQAPTASSQQAALESTQGVSQVAPPEPVSVAQPQPTQPAALVSSIDSAHSDVASGMSDGNENVPSSGGRHEGRTAKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPLPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEMSDTVAASTSHGAGVNLSHSASSLSLQQAFSELRHAQVTEGPNTAPPNFSHTGPTFPVVPPSMSSIAGVPKTTAAAPSISVPVTSCPLNDVSTSIIQPEITVPTEKGVAGVATCTGVITSSGFPVPPASESPVLSSVVSSITIPTIVSISTTSQSVQAPTSGSVVSSVGTFPSIPVSATSASAVGGAATPGAKSPPLLSQQSAGITTGIATLTSVSTTIPFPSTASQLSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTAGLALSHSASSSTSSPGAGVSSSISQSGGVHPLVVPSAIASTPVLPQASVPISTPLLPQGPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEIDVDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVALETSLVVETSVTPGIPTTAVAPSKLMTSTTSTCLPPTSLPLGTAGLSVTPLVTTGQVSTPVGTTSGVKPGTAPSKLPLTKAPVLPVGTELPAGTPSSEQLPPIPGPSLTQSQQPLEDLDAQLRRALSPETIAVTSAVGPVSVVAPAAVTEAGAQPQKDVSQVTGGSVPATSSGTGVFKTGRFQVSVAIDDSQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPGSNGVTIHGISLDMPDSAHKTPAAEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDRITEAKKEGPTASPPFMDLEQAVLPAAIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSLNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRISSLGSKSPQLSGNMSGQSEASVLHPQQTLHPPGNIPETGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPPTPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_016131759.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016131759.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCGGA GAAGCAGAGC AGCACTCCGA GCTCTCTGTT CCTCTCGCCG 
CCTGCGCCTT CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CTGTGGGGGA GAAACTGGGC 
ACGGCGGGAA CCGACGCTGG AACTGGCAGG ACTGAGGAGT ATCGGCGTCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG AGCAGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC 
CGCAGCGTCA TCTGCGACTC CAATGCCACT GCATTGGAAC TGCCGGGTCT CCCACTTTCT 
CTGCCCCAGC CCAGCGTCGC CGCGGTGGTT CCGCAGAGTA CTCCACCAGA GCTCCAGCGC 
GAAGAGACCT TGACCGCTAC CGCCGCTGCC CAGGTAGCCC AGCAGCCTCC CACCGCTTCT 
ACTGCTGTCT CTGGGGAACC GGCCGCCGCG GGCCCTGTCG CCACGACTGC CCCCAGCAGT 
ACCAGCAAAG ACCGCCAAGT TTCTCAGCCC AACCATGTGG GGAGCAAGGA GGAGCCTCCG 
TCGGCGAGAA GTGGCAGCGG CGGCGGCAGC AGCGCCAAAG AACCACAGGA GGAACGGAGC 
CAGCAACAGG ATGATATCGA AGAGTTGGAG ACCAAGGCCG TGGGAATGTC CAATGATGGC 
CGCTTTCTCA AGTTTGACAT CGAAATTGGC AGAGGCTCCT TTAAGACGGT CTACAAAGGT 
TTAGACACTG AAACCACTGT GGAGGTCGCC TGGTGTGAAC TACAGGACCG CAAGTTAACA 
AAGTCTGAGA GGCAGAGATT TAAAGAAGAG GCTGAAATGT TAAAGGGTCT TCAGCATCCC 
AATATTGTTC GATTCTATGA TTCCTGGGAA TCCACAGTAA AAGGAAAGAA GTGCATTGTT 
TTGGTGACTG AACTTATGAC ATCTGGAACA CTTAAAACGT ATCTGAAAAG GTTTAAGGTG 
ATGAAGATCA AAGTTTTAAG AAGCTGGTGC CGTCAGATCC TTAAAGGACT TCAATTTCTT 
CATACTCGAA CTCCACCTAT TATTCACCGA GATCTTAAGT GTGACAACAT CTTTATCACT 
GGCCCTACAG GCTCAGTCAA GATTGGAGAC TTAGGTCTGG CAACCCTGAA GCGAGCTTCT 
TTTGCCAAGA GTGTGATAGG TACCCCTGAG TTCATGGCCC CTGAGATGTA TGAGGAGAAA 
TATGATGAAT CTGTTGACGT TTATGCTTTT GGGATGTGCA TGCTTGAGAT GGCCACATCT 
GAATACCCTT ACTCAGAGTG CCAAAATGCT GCGCAGATCT ACCGTCGAGT GACCAGTGGG 
GTGAAACCAG CCAGTTTTGA CAAAGTAGCA ATTCCTGAAG TGAAGGAAAT TATTGAAGGT 
TGCATTCGGC AAAACAAAGA TGAAAGATAC TCCATCAAAG ACCTTTTGAA CCATGCCTTC 
TTCCAGGAGG AAACAGGGGT GCGGGTAGAA TTAGCAGAAG AAGATGATGG AGAAAAGATA 
GCCATTAAAT TATGGCTACG TATTGAAGAT ATTAAGAAAT TAAAGGGAAA ATACAAAGAC 
AATGAAGCTA TTGAGTTTTC CTTTGACCTG GAGAGGGATG TGCCAGAAGA TGTTGCTCAA 
GAAATGGTAG AGTCTGGGTA TGTCTGTGAA GGTGATCACA AGACTATGGC AAAAGCCATC 
AAAGATAGAG TGTCTTTAAT TAAGAGAAAA CGAGAGCAGC GGCAATTGGT GCGAGAGGAG 
CAAGAAAAAA GAAAGCAGGA AGAGAGCAGT CTCAAACAGC AAGTAGAACA GCAACCAAGT 
GCTTCCCAGG CAGGAATCAA GCAGCTCTCT TCTGCTAGCA CCGGCATACC TACTGCCTCT 
GCCACTTCAG CTTCAGTTTC TACACAAGTA GAACCTGAAG AACCTGAGGC AGACCAACAT 
CAACAACTAC AGTACCAACA ACCTAGTGTA TCTGTGTTAT CCGATGGGAC TGTTGACAGT 
GGTCAGGGAT CTTCTGTCTT CACAGAATCT CGAGTCAGCA GCCAACAGAC AGTTTCATAT 
GGTTCCCAAC ATGAACAGGC ACATTCTACT GGCACATTCC CAGGGCATAC AGCTTCTGTT 
GTCCAAGCAC AATCTCAGCC CCATGGTGTA TATCCACCCT CAAGTATGCC TCAGTCCATG 
GCGCATCCGT GTGGGGGGAC CCCAACATAC CCAGAATCAC AGATATTTTT CCCAACTATT 
CATGAACGTC CAGTTTCTTT TTCACCACCT CCCACCTGTC CACTGAAGGT AGCCATTTCC 
CAGCGACGTA AGAGCACCTC CTTCCTGGAA GCTCAAACTC ACCACTTCCA ACCCCTGCTG 
AGGACTGTTG GCCAAAATCT TCTTCCACCT GGTGGCAGCC CAACTAACTG GACACCAGAG 
GCCGTAGTTA TGCTGGGTTC TACAACCAGT AGAATATCCG GAGAGCCATG TGATATACAG 
GTCAATTCTA TATGTGAACC AGCTCAAGTG TACAGTGACT ATAGACCTGG ACTAGTACTT 
CCAGAAGAAG CTCACTATTT TATTCCTCAG GAAGCAGTGT ATCTAGCTGG GGTGCAGTAC 
CAGGCCCAGG TGGCAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACTGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACCTC GAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCTTG GTAGGACACC TTCAGAATTT AAGATTAGAT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CACCTGCTGT GTTAACTCAT 
AACAATGAGA ACAGAAGCAA TTGTGTATTT GAATTTCATG TTCACACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAGT TTTATCTCAG CGTGTTTACC GAAATCGACA GGTTGCGGTG 
GACTTGAATC AGGAAGAACT ACCTCCTCAA TCAGTTGGAG TACATGGCCG CCTGCAGCCT 
GTGACTGAAG AACAGCATAA TTACCATGTC CCAGAATTGA CTGTTTCTGT GGTAGAGCCT 
ATCGGACAGA ACTGGCCAAT AGGAAGCCCA GAATATTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAGTCACCT CCCCCTACAG GGGGAGCACC TGCATCTTTT 
GGCTCTGACG TCTCATTACC CTTTATCCGT CTACCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGA TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAGA GCCAGGGTCA GCCATCCTCA 
AGTAGTGTAA CAGGGGTTTC ATCTTCCCAA TCCATACAAC ATTCTCAGCA GCAGCATGGA 
ATACAACAGA CAGCCCCTCC TCAACAGACA GTGCAGTATT CACTTCCACA GACATCAACC 
TCCAGTGAGG CCACTACTGC ACAGCCAGTG AGTCAGCCTC AAGCTCCACA GGTCTTGCCT 
CAAGCATCAG CTGGAAAACA GGGCTTCCCA CCTCGACTGC CACCACAATA CCCAGGAGAT 
TCAAATATTG CTTCCTCTTC CAGCGTGGCT TCTGTTTCCA TTCCTTCTAC AGTCCTATCC 
CCTCCAATGC CGACAGAAGC ATTAGCTACA CCAGGTTACT TTCCTACAGT GGTGCAGCCT 
TATATGGAAT CAAATCTTAT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAACCAGCAG TGAGTTTAGC ACAAGCCCCC ACTGCATCCT CCCAGCAAGC AGCTCTGGAG 
AGTACTCAGG GAGTCTCTCA GGTTGCTCCT CCAGAGCCAG TTTCGGTAGC ACAGCCACAG 
CCCACCCAGC CTGCCGCTTT GGTTTCTTCT ATAGACAGTG CTCATTCAGA TGTCGCTTCG 
GGTATGAGCG ATGGCAATGA GAATGTCCCA TCATCTGGTG GAAGGCATGA AGGGAGAACT 
GCAAAACGGC ATTATCGAAA ATCTGTAAGA AGCCGCTCTC GTCATGAAAA GACTTCACGC 
CCCAAATTGA GAATTTTGAA TGTTTCAAAT AAAGGAGACC GGGTAGTAGA ATGTCAATTA 
GAGACTCATA ATCGGAAAAT GGTTACATTC AAATTTGATT TAGATGGTGA CAATCCTGAG 
GAGATAGCAA CAATTATGGT GAACAATGAC TTTATTCTAG CAATAGAGAG AGAGTCATTT 
GTGGATCAAG TGCGGGAAAT TATTGAAAAA GCTGATGAAA TGCTCAGTGA GGATGTCAGT 
GTGGAACCTG AGGGTGACCA GGGATTGGAG AGTCTACAGG GAAAGGATGA CTATGGCTTT 
TCAGGTTCTC AGAAATTGGA GGGAGAATTC AAACAACCAC TTCCTGCATC TTCCATGCCA 
CAGCAAATAG GAATTCCTAC CAGTTCTTTA ACTCAAGTTG TTCATTCTGC CGGAAGACGG 
TTTATAGTGA GTCCTGTGCC AGAAAGCCGA TTACGAGAAT CAAAAGTTTT CACCAGTGAA 
ATGTCAGATA CAGTTGCCGC CTCTACATCT CATGGCGCTG GGGTGAACTT GTCCCACTCT 
GCTTCTTCCC TTAGTCTACA GCAGGCCTTT TCTGAACTCA GACACGCCCA AGTGACAGAG 
GGACCCAATA CAGCACCTCC CAACTTCAGT CATACAGGAC CAACATTTCC AGTAGTACCT 
CCTTCCATGA GTAGCATTGC TGGAGTCCCA AAGACAACAG CAGCCGCACC TTCAATATCA 
GTCCCTGTAA CTAGCTGCCC TCTTAATGAC GTTTCCACGT CAATAATTCA GCCTGAGATT 
ACAGTGCCCA CTGAAAAAGG AGTTGCTGGA GTTGCCACCT GCACAGGTGT AATAACTTCA 
AGTGGTTTCC CAGTACCCCC TGCATCTGAA TCACCAGTTC TTTCCAGTGT GGTTTCAAGT 
ATCACAATAC CGACAATTGT CTCAATATCA ACAACATCCC AGTCAGTTCA GGCTCCCACA 
TCTGGGAGCG TTGTTTCTAG TGTAGGCACT TTCCCTTCTA TACCAGTTTC AGCAACTTCA 
GCCTCTGCAG TGGGCGGTGC TGCTACCCCA GGGGCTAAGT CTCCACCTCT ATTATCTCAG 
CAGTCAGCAG GCATTACTAC AGGGATAGCC ACATTAACAT CAGTTTCTAC CACCATTCCA 
TTTCCAAGCA CAGCTTCACA GCTATCTCTT CAGCTTAGCA GCAGCACCTC TGCTCCTACT 
CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCATTAGATA AAACATCTCA TAGCAGTACA 
GCTGGATTGG CTTTGTCCCA CTCTGCATCA TCTTCCACTT CTTCCCCTGG AGCAGGAGTA 
TCTAGTTCTA TTTCTCAGTC TGGTGGGGTA CATCCTTTGG TCGTCCCATC AGCTATAGCT 
TCTACTCCTG TCCTTCCCCA GGCATCAGTA CCTATTTCTA CACCTTTATT ACCCCAAGGA 
CCTAGCATCC CACCATTGGT GCAACCTGTT GCCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AGCCTCAACC AGCTTTGCTT CCCAACCAGC CCCATACACA CTGCCCTGAA 
ATAGATGTTG ATACACAACC CAAAGCTCCT GGGATTGATG ACATAAAGAC TTTAGAGGAA 
AAGTTGCGTT CCCTCTTTAG TGAACACAGC TCTTCTGGCG CTCAACATGC CTCTGTTGCA 
CTGGAGACAT CACTAGTAGT AGAGACTAGT GTCACACCAG GCATCCCAAC CACTGCTGTT 
GCACCCAGCA AACTCATGAC TTCAACTACA AGTACTTGCT TACCACCAAC CAGTTTGCCA 
TTAGGAACAG CTGGTTTGTC CGTTACACCA CTGGTCACAA CTGGGCAAGT TTCTACCCCC 
GTCGGCACTA CATCAGGAGT GAAACCTGGA ACTGCTCCAT CAAAGCTACC TTTAACTAAG 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCATCCAG TGAGCAGCTG 
CCACCTATTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTAAGAA GAGCACTTAG TCCAGAGACT ATTGCGGTGA CATCCGCAGT TGGTCCTGTG 
TCCGTGGTGG CTCCAGCGGC AGTTACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAAGTCACAG GAGGTTCTGT CCCAGCAACT AGTTCAGGAA CTGGTGTTTT TAAGACGGGG 
CGATTTCAGG TTTCAGTTGC AATAGATGAT TCCCAAAAAG AGGGTAAAAA TAAGTCAGAA 
GATGCAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAGA GTACCCTGGT GAAGCCAGGC TCTAATGGGG TAACCATCCA TGGTATTTCT 
TTAGATATGC CAGATAGTGC CCACAAAACT CCTGCTGCAG AAGCAAAGTC AGAAACTGGG 
CAACCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTATCAA GAACTGAAGA CAGGATCACT GAGGCAAAGA AAGAGGGACC GACGGCATCT 
CCTCCTTTTA TGGATTTGGA ACAAGCTGTT CTTCCTGCTG CGATACCAAA GAAAGAAAAA 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGGCCATCTT CTGATTTGGA GGCCGCCTTC 
CTAAGTAGGG ATGTAGATGA TGGTTCAGGT AGTCCACACT CTCCTCACCA ACTGAGCTCA 
AAGAGTCTAC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ACTCACTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGACT TAAAGTTAGA GCTGCGACGA 
CTACGAGACA AACATCTTAA AGAGATTCAA GACCTACAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACTAAACT GGGCAAGGTT CCTCCTGCTG TCATTATTCC CCCAGCGGCC 
CCCCTTTCAG GAAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAATC TAGTCGTATC 
AGTTCCTTGG GGAGTAAAAG CCCCCAGCTT TCAGGTAATA TGTCTGGTCA GAGTGAAGCT 
TCAGTCTTGC ACCCCCAGCA GACCCTCCAC CCTCCCGGCA ACATCCCAGA AACTGGGCAG 
AATCAGTTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTGTA TTCAGCCTTC 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGTCAAGG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC AAGCTCAGCC TCCTGCCATG 
ACGTCCAGCA GGAAGGGCAC ATTCACTGAT GACCTGCACA AGTTGGTAGA CAATTGGGCC 
CGAGACGCCA TGAATCTTTC AGGCAGGAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGGAAGTT CTCTGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAC 
CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCACCTACCC CCTTTGGCAC TCAGTGGAGT 
GGGACAGGTG GCCCAGCACC ACAGCCACTT GGCCAGTTCC AACCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGAGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK1
Entrez Gene ID	107503449
Full Name	WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Rousettus aegyptiacus(Egyptian rousette)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK1 cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette)

Gene

Clones

gRNAs

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