ROBO4 cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROBO4
Entrez Gene ID	107498946
Full Name	roundabout guidance receptor 4
Gene Type	protein-coding
Organism	Rousettus aegyptiacus(Egyptian rousette)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016122654.1	XP_015978140.1	roundabout homolog 4 isoform X1
XM_016122655.1	XP_015978141.1	roundabout homolog 4 isoform X2

Pathways from BioSystems
Gene Ontology

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORo30258	XM_016122655.1 Latest version!	Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORo30257	XM_016122654.1 Latest version!	Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROBO4 ( XM_016122655.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015978141.1 Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORo30258
Clone ID Related Accession (Same CDS sequence)	XM_016122655.1
Accession Version	XM_016122655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	roundabout homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015494953.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG GAGGAGCCGG CCTCCTTGGA GCCTGGTGGC CCCTGCCTCT CCTGCTTCTG 
CTCAGCTCAG GAGGCAAGGC TCAGGACTCC CCACCTCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCCGG CCCCGCCAAG ATGAGCTGCC GGGCTTCAGG CCAGCCACCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCTCTGAGTA TGGTGCCCCC AGACGTACAC 
CACCTCCTAC CTGATGGAAC CCTGCTGCTG CTGCGGCCCC CAGCCCAGGG ACGTGCCCAC 
AAGGACCAGG CCCGATCCAC AGACCTAGGC ATCTATACCT GTGAGGCCAG CAACCGGCTG 
GGCACGGCCG TCAGCCGAGG TGCTCGGCTG TCTGTGGCTG TCCTTTGGGA GGATTTCCAG 
ATCCATCCAC GGGACACAGT GGCCACGGTG GGTGAGCAGG TGACCCTGGA GTGTAGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCACA TGGTGGAAGG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGGC GGCACACGGT ATCCAGGGGT TCCTTGCTGA TGATAAGAGC GGAGAAGAGC 
GACGCGGGGA CCTATATGTG CGTGGCCACC AACGACGCGG GACGACGGGA GAGCCGGGCA 
GCCAGGGTGT CGGTCCAGGA GCCCCGGGAC TACAAGGAGC CTCTGGAGAT TCTGGCTATG 
CACATTCGGC TGGAAAACGT GACCTTGATG AACCCAAACC CCACAAAGGG CACCAAGCCT 
GGGCCTGCTG TATGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCGCCTGC TCAGTCCTAC 
ACAGCGCTGT TCAGGGCCCA GACAGCCTCG GGAGGCCAGG GAGCTCCATG GGCAGAGGCC 
CTGCTGGCTG GCTGGCAGAG CGCAGAGCTT GGCGGCCTCC GCTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCCG AACAAGTGCC CAGTGCCCCT CCCCAGGAGG TGACCCTAAA ACTTGGCAAT 
GGCAGTGTCC TTGTGAACTG GGTTCCACCA CCAGCTGAAA ACCACAATGG CGTCATCCGT 
GGCTACCAGG TATGGAGTCT GGGAAACTCC TCGCTCCTCC CAACCAACTG GACAGTGGCT 
GCCGAGCAGA CCCAGCTGGA GATTGCCACC CAAATGCCAG GTTCCTACTG TGTGCAAGTA 
GCTGCAGTCA CTGGTGCTGG GGCTGGGGAG CCCAGTAGCC CCGTTTGCCT CATTTTAGAG 
CAGGCCATGG AGCGAGCTGC CCGAGAACCA AATGACCATG GTCCATGGAC TCTGGAGCAG 
CTGAGAGCTA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGTGTATTGT GCTCTGGCTG 
CTGCTGCTTG GCACTGCAGT GTGTGTCCAC CACCGGCGCC GAGGTGCGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCTCCCT GGCTGGCAGA CACTTGGCAG TCCACCTCTG GCTCTCGCGA CCTGAGCAGC 
AGCAGTAGCC TCAGCAGCCG GCTAGGAATA GACCCCCGGG ATCCACTAGG CTGTCCTCGT 
TCCTTGATCT CCTGGGATCC CCGCAGCCCC GGAGTGCCCC TGCTTCCCGA CACCAGCACT 
TTCTATGGCT CCCTCATCAC TGAACTGCCT TCCAGCCCTC CAGCCCGGCC GAGCCCCCAT 
GCCCCAACTG TCAAGCGTCT CCCACCCCAG CTGGCCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCAGCCG CAGGGGGCTC TCTTCTCCGC GCTTGTCCAT GGCCCCCACA 
GAGGCTTGGA AGGCCAAAAA GAAGCAAGAA CTGCACCAAG CCAACAGCTC CCCATTGCTC 
CGGGCCAGCC ACCCTATGGA GCACTGGGCC TGGGAGCTGG GGAAAAAAGG CTCCAAGAAC 
CTTTCTCAAA GCCCAGGAGC TGCGCCCCGA GCTCTGGTTG CCTGGCGGGC CCTGGGACCA 
CAGCTTCTCA ACTCCTCCAG TGAGCTGACT CGCCCTCTCC CCACAGCACC CCCCTCTTCT 
CATGGAGTCC CCACTCAGAA TCAACCGACC CTGCGCTCGG TGGAGCTCCA AGCTCCCCCC 
TCCCACCCAC TGCCAGCAGC CCCCAGCCCC ATCCTCATCC CCTCCAGTCC CAAGGCCTCT 
TCCCTCTCTG GTCCCAGCCC AGCCTCCAGT GGCCTGTCCA GCTCTTCACT GTCATCTCTG 
GGGGACGATC AGGACAGTGT GCTGACTCCT GAGGAGGTGG CCCTTTGCCT GGAGTTCAGT 
GAGGGTGAAG AGACCCCCAG GAACAGTGTC TCTCCTATGC CAAGGGCTCC TTCGCCCCCC 
GTCACCTATG GGTACATCAG CGTCCCGACA GCCTCAGAGC TAGCAGACAT AGACAGGGCT 
GGAGGAGGGG TGGGGTCTGA GGTGAGGGGC TTGTTGTGCC CCCCTCGGCC CTGCCTCACC 
CCTACTCCCA GTGAGGGCTC CCTGGCCAAT GGCTGGGGCT CAGCCTCCGA GGACAATGCC 
CCCAGCGCCA GAACCAGCCT CGTCAGCTCT TCTGATGGCT CCTTCCTCGC GGATGCCCAC 
TTTGCAAGGG CCCTGGCAGT GGCTGTGGAC AGCCTTGGCT TTGGTCTGGA GCCCAAGGAG 
TCAGACTGCA TCTTCACAGA TGCTTCATCG CCTCCCTCCC CCCGGGATGA CGTCTTCCTG 
ACCCCTACCT TCTCCCTGCC CCTGTGGGAA TGGAGATCAG ACTGGCTAGA GGATGTGGAG 
AGCAACCACA CCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCTCCGGA CTCTCAGATC 
TCTTCCCAAA GAAGTCAGCG CAGCTGTCCT GTGTCCAAGG CTGGCAACTC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015978141.1
CDS	129..3125
Translation	MGSGGAGLLGAWWPLPLLLLLSSGGKAQDSPPQILVHPQDQLFQGPGPAKMSCRASGQPPPTIRWLLNGQPLSMVPPDVHHLLPDGTLLLLRPPAQGRAHKDQARSTDLGIYTCEASNRLGTAVSRGARLSVAVLWEDFQIHPRDTVATVGEQVTLECRPPWGHPEPTVTWWKDGKPLALQPGRHTVSRGSLLMIRAEKSDAGTYMCVATNDAGRRESRAARVSVQEPRDYKEPLEILAMHIRLENVTLMNPNPTKGTKPGPAVWLSWKVSGPAAPAQSYTALFRAQTASGGQGAPWAEALLAGWQSAELGGLRWGQDYEFKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLKLGNGSVLVNWVPPPAENHNGVIRGYQVWSLGNSSLLPTNWTVAAEQTQLEIATQMPGSYCVQVAAVTGAGAGEPSSPVCLILEQAMERAAREPNDHGPWTLEQLRATLRRPEVIASGCIVLWLLLLGTAVCVHHRRRGAVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWQSTSGSRDLSSSSSLSSRLGIDPRDPLGCPRSLISWDPRSPGVPLLPDTSTFYGSLITELPSSPPARPSPHAPTVKRLPPQLARLSSPWPSSDSLCSRRGLSSPRLSMAPTEAWKAKKKQELHQANSSPLLRASHPMEHWAWELGKKGSKNLSQSPGAAPRALVAWRALGPQLLNSSSELTRPLPTAPPSSHGVPTQNQPTLRSVELQAPPSHPLPAAPSPILIPSSPKASSLSGPSPASSGLSSSSLSSLGDDQDSVLTPEEVALCLEFSEGEETPRNSVSPMPRAPSPPVTYGYISVPTASELADIDRAGGGVGSEVRGLLCPPRPCLTPTPSEGSLANGWGSASEDNAPSARTSLVSSSDGSFLADAHFARALAVAVDSLGFGLEPKESDCIFTDASSPPSPRDDVFLTPTFSLPLWEWRSDWLEDVESNHTQRLGRGLPPWPPDSQISSQRSQRSCPVSKAGNSS

Target ORF information:

RefSeq Version	XM_016122655.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016122655.1

ORF Insert Sequence:

ATGGGCTCCG GAGGAGCCGG CCTCCTTGGA GCCTGGTGGC CCCTGCCTCT CCTGCTTCTG 
CTCAGCTCAG GAGGCAAGGC TCAGGACTCC CCACCTCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCCGG CCCCGCCAAG ATGAGCTGCC GGGCTTCAGG CCAGCCACCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCTCTGAGTA TGGTGCCCCC AGACGTACAC 
CACCTCCTAC CTGATGGAAC CCTGCTGCTG CTGCGGCCCC CAGCCCAGGG ACGTGCCCAC 
AAGGACCAGG CCCGATCCAC AGACCTAGGC ATCTATACCT GTGAGGCCAG CAACCGGCTG 
GGCACGGCCG TCAGCCGAGG TGCTCGGCTG TCTGTGGCTG TCCTTTGGGA GGATTTCCAG 
ATCCATCCAC GGGACACAGT GGCCACGGTG GGTGAGCAGG TGACCCTGGA GTGTAGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCACA TGGTGGAAGG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGGC GGCACACGGT ATCCAGGGGT TCCTTGCTGA TGATAAGAGC GGAGAAGAGC 
GACGCGGGGA CCTATATGTG CGTGGCCACC AACGACGCGG GACGACGGGA GAGCCGGGCA 
GCCAGGGTGT CGGTCCAGGA GCCCCGGGAC TACAAGGAGC CTCTGGAGAT TCTGGCTATG 
CACATTCGGC TGGAAAACGT GACCTTGATG AACCCAAACC CCACAAAGGG CACCAAGCCT 
GGGCCTGCTG TATGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCGCCTGC TCAGTCCTAC 
ACAGCGCTGT TCAGGGCCCA GACAGCCTCG GGAGGCCAGG GAGCTCCATG GGCAGAGGCC 
CTGCTGGCTG GCTGGCAGAG CGCAGAGCTT GGCGGCCTCC GCTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCCG AACAAGTGCC CAGTGCCCCT CCCCAGGAGG TGACCCTAAA ACTTGGCAAT 
GGCAGTGTCC TTGTGAACTG GGTTCCACCA CCAGCTGAAA ACCACAATGG CGTCATCCGT 
GGCTACCAGG TATGGAGTCT GGGAAACTCC TCGCTCCTCC CAACCAACTG GACAGTGGCT 
GCCGAGCAGA CCCAGCTGGA GATTGCCACC CAAATGCCAG GTTCCTACTG TGTGCAAGTA 
GCTGCAGTCA CTGGTGCTGG GGCTGGGGAG CCCAGTAGCC CCGTTTGCCT CATTTTAGAG 
CAGGCCATGG AGCGAGCTGC CCGAGAACCA AATGACCATG GTCCATGGAC TCTGGAGCAG 
CTGAGAGCTA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGTGTATTGT GCTCTGGCTG 
CTGCTGCTTG GCACTGCAGT GTGTGTCCAC CACCGGCGCC GAGGTGCGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCTCCCT GGCTGGCAGA CACTTGGCAG TCCACCTCTG GCTCTCGCGA CCTGAGCAGC 
AGCAGTAGCC TCAGCAGCCG GCTAGGAATA GACCCCCGGG ATCCACTAGG CTGTCCTCGT 
TCCTTGATCT CCTGGGATCC CCGCAGCCCC GGAGTGCCCC TGCTTCCCGA CACCAGCACT 
TTCTATGGCT CCCTCATCAC TGAACTGCCT TCCAGCCCTC CAGCCCGGCC GAGCCCCCAT 
GCCCCAACTG TCAAGCGTCT CCCACCCCAG CTGGCCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCAGCCG CAGGGGGCTC TCTTCTCCGC GCTTGTCCAT GGCCCCCACA 
GAGGCTTGGA AGGCCAAAAA GAAGCAAGAA CTGCACCAAG CCAACAGCTC CCCATTGCTC 
CGGGCCAGCC ACCCTATGGA GCACTGGGCC TGGGAGCTGG GGAAAAAAGG CTCCAAGAAC 
CTTTCTCAAA GCCCAGGAGC TGCGCCCCGA GCTCTGGTTG CCTGGCGGGC CCTGGGACCA 
CAGCTTCTCA ACTCCTCCAG TGAGCTGACT CGCCCTCTCC CCACAGCACC CCCCTCTTCT 
CATGGAGTCC CCACTCAGAA TCAACCGACC CTGCGCTCGG TGGAGCTCCA AGCTCCCCCC 
TCCCACCCAC TGCCAGCAGC CCCCAGCCCC ATCCTCATCC CCTCCAGTCC CAAGGCCTCT 
TCCCTCTCTG GTCCCAGCCC AGCCTCCAGT GGCCTGTCCA GCTCTTCACT GTCATCTCTG 
GGGGACGATC AGGACAGTGT GCTGACTCCT GAGGAGGTGG CCCTTTGCCT GGAGTTCAGT 
GAGGGTGAAG AGACCCCCAG GAACAGTGTC TCTCCTATGC CAAGGGCTCC TTCGCCCCCC 
GTCACCTATG GGTACATCAG CGTCCCGACA GCCTCAGAGC TAGCAGACAT AGACAGGGCT 
GGAGGAGGGG TGGGGTCTGA GGTGAGGGGC TTGTTGTGCC CCCCTCGGCC CTGCCTCACC 
CCTACTCCCA GTGAGGGCTC CCTGGCCAAT GGCTGGGGCT CAGCCTCCGA GGACAATGCC 
CCCAGCGCCA GAACCAGCCT CGTCAGCTCT TCTGATGGCT CCTTCCTCGC GGATGCCCAC 
TTTGCAAGGG CCCTGGCAGT GGCTGTGGAC AGCCTTGGCT TTGGTCTGGA GCCCAAGGAG 
TCAGACTGCA TCTTCACAGA TGCTTCATCG CCTCCCTCCC CCCGGGATGA CGTCTTCCTG 
ACCCCTACCT TCTCCCTGCC CCTGTGGGAA TGGAGATCAG ACTGGCTAGA GGATGTGGAG 
AGCAACCACA CCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCTCCGGA CTCTCAGATC 
TCTTCCCAAA GAAGTCAGCG CAGCTGTCCT GTGTCCAAGG CTGGCAACTC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( XM_016122654.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_015978140.1 Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORo30257
Clone ID Related Accession (Same CDS sequence)	XM_016122654.1
Accession Version	XM_016122654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015494953.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCG GAGGAGCCGG CCTCCTTGGA GCCTGGTGGC CCCTGCCTCT CCTGCTTCTG 
CTCAGCTCAG GAGGCAAGGC TCAGGACTCC CCACCTCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCCGG CCCCGCCAAG ATGAGCTGCC GGGCTTCAGG CCAGCCACCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCTCTGAGTA TGGTGCCCCC AGACGTACAC 
CACCTCCTAC CTGATGGAAC CCTGCTGCTG CTGCGGCCCC CAGCCCAGGG ACGTGCCCAC 
AAGGACCAGG CCCGATCCAC AGACCTAGGC ATCTATACCT GTGAGGCCAG CAACCGGCTG 
GGCACGGCCG TCAGCCGAGG TGCTCGGCTG TCTGTGGCTG TCCTTTGGGA GGATTTCCAG 
ATCCATCCAC GGGACACAGT GGCCACGGTG GGTGAGCAGG TGACCCTGGA GTGTAGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCACA TGGTGGAAGG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGGC GGCACACGGT ATCCAGGGGT TCCTTGCTGA TGATAAGAGC GGAGAAGAGC 
GACGCGGGGA CCTATATGTG CGTGGCCACC AACGACGCGG GACGACGGGA GAGCCGGGCA 
GCCAGGGTGT CGGTCCAGGA GCCCCGGGAC TACAAGGAGC CTCTGGAGAT TCTGGCTATG 
CACATTCGGC TGGAAAACGT GACCTTGATG AACCCAAACC CCACAAAGGG CACCAAGCCT 
GGGCCTGCTG TATGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCGCCTGC TCAGTCCTAC 
ACAGCGCTGT TCAGGGCCCA GACAGCCTCG GGAGGCCAGG GAGCTCCATG GGCAGAGGCC 
CTGCTGGCTG GCTGGCAGAG CGCAGAGCTT GGCGGCCTCC GCTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCCG AACAAGTGCC CAGTGCCCCT CCCCAGGAGG TGACCCTAAA ACTTGGCAAT 
GGCAGTGTCC TTGTGAACTG GGTTCCACCA CCAGCTGAAA ACCACAATGG CGTCATCCGT 
GGCTACCAGG TATGGAGTCT GGGAAACTCC TCGCTCCTCC CAACCAACTG GACAGTGGCT 
GCCGAGCAGA CCCAGCTGGA GATTGCCACC CAAATGCCAG GTTCCTACTG TGTGCAAGTA 
GCTGCAGTCA CTGGTGCTGG GGCTGGGGAG CCCAGTAGCC CCGTTTGCCT CATTTTAGAG 
CAGGCCATGG AGCGAGCTGC CCGAGAACCA AATGACCATG GTCCATGGAC TCTGGAGCAG 
CTGAGAGCTA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGTGTATTGT GCTCTGGCTG 
CTGCTGCTTG GCACTGCAGT GTGTGTCCAC CACCGGCGCC GAGGTGCGGT GCACCTGGGC 
CCAGGTGAGA GGGTAAACTG GACCCCAGAT AGGCAGGCCT CCCTGGGTCC GGTGAGAACG 
CTGAGGGAGG TTCGATCTCT AGCCAGGTGT TGGCAACTCC AGATTTGTCC TTCTTTCTGC 
CCTCTCTCAG GTCTGTACAG ATATACCAGT GAGGATGCCA TCCTAAAACA CAGGATGGAT 
CACAGTGACT CTCCCTGGCT GGCAGACACT TGGCAGTCCA CCTCTGGCTC TCGCGACCTG 
AGCAGCAGCA GTAGCCTCAG CAGCCGGCTA GGAATAGACC CCCGGGATCC ACTAGGCTGT 
CCTCGTTCCT TGATCTCCTG GGATCCCCGC AGCCCCGGAG TGCCCCTGCT TCCCGACACC 
AGCACTTTCT ATGGCTCCCT CATCACTGAA CTGCCTTCCA GCCCTCCAGC CCGGCCGAGC 
CCCCATGCCC CAACTGTCAA GCGTCTCCCA CCCCAGCTGG CCCGGCTCTC CAGCCCCTGG 
CCCAGTTCAG ACAGCCTCTG CAGCCGCAGG GGGCTCTCTT CTCCGCGCTT GTCCATGGCC 
CCCACAGAGG CTTGGAAGGC CAAAAAGAAG CAAGAACTGC ACCAAGCCAA CAGCTCCCCA 
TTGCTCCGGG CCAGCCACCC TATGGAGCAC TGGGCCTGGG AGCTGGGGAA AAAAGGCTCC 
AAGAACCTTT CTCAAAGCCC AGGAGCTGCG CCCCGAGCTC TGGTTGCCTG GCGGGCCCTG 
GGACCACAGC TTCTCAACTC CTCCAGTGAG CTGACTCGCC CTCTCCCCAC AGCACCCCCC 
TCTTCTCATG GAGTCCCCAC TCAGAATCAA CCGACCCTGC GCTCGGTGGA GCTCCAAGCT 
CCCCCCTCCC ACCCACTGCC AGCAGCCCCC AGCCCCATCC TCATCCCCTC CAGTCCCAAG 
GCCTCTTCCC TCTCTGGTCC CAGCCCAGCC TCCAGTGGCC TGTCCAGCTC TTCACTGTCA 
TCTCTGGGGG ACGATCAGGA CAGTGTGCTG ACTCCTGAGG AGGTGGCCCT TTGCCTGGAG 
TTCAGTGAGG GTGAAGAGAC CCCCAGGAAC AGTGTCTCTC CTATGCCAAG GGCTCCTTCG 
CCCCCCGTCA CCTATGGGTA CATCAGCGTC CCGACAGCCT CAGAGCTAGC AGACATAGAC 
AGGGCTGGAG GAGGGGTGGG GTCTGAGGTG AGGGGCTTGT TGTGCCCCCC TCGGCCCTGC 
CTCACCCCTA CTCCCAGTGA GGGCTCCCTG GCCAATGGCT GGGGCTCAGC CTCCGAGGAC 
AATGCCCCCA GCGCCAGAAC CAGCCTCGTC AGCTCTTCTG ATGGCTCCTT CCTCGCGGAT 
GCCCACTTTG CAAGGGCCCT GGCAGTGGCT GTGGACAGCC TTGGCTTTGG TCTGGAGCCC 
AAGGAGTCAG ACTGCATCTT CACAGATGCT TCATCGCCTC CCTCCCCCCG GGATGACGTC 
TTCCTGACCC CTACCTTCTC CCTGCCCCTG TGGGAATGGA GATCAGACTG GCTAGAGGAT 
GTGGAGAGCA ACCACACCCA GCGGCTGGGA AGGGGGCTGC CTCCCTGGCC TCCGGACTCT 
CAGATCTCTT CCCAAAGAAG TCAGCGCAGC TGTCCTGTGT CCAAGGCTGG CAACTCCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015978140.1
CDS	54..3176
Translation	MGSGGAGLLGAWWPLPLLLLLSSGGKAQDSPPQILVHPQDQLFQGPGPAKMSCRASGQPPPTIRWLLNGQPLSMVPPDVHHLLPDGTLLLLRPPAQGRAHKDQARSTDLGIYTCEASNRLGTAVSRGARLSVAVLWEDFQIHPRDTVATVGEQVTLECRPPWGHPEPTVTWWKDGKPLALQPGRHTVSRGSLLMIRAEKSDAGTYMCVATNDAGRRESRAARVSVQEPRDYKEPLEILAMHIRLENVTLMNPNPTKGTKPGPAVWLSWKVSGPAAPAQSYTALFRAQTASGGQGAPWAEALLAGWQSAELGGLRWGQDYEFKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLKLGNGSVLVNWVPPPAENHNGVIRGYQVWSLGNSSLLPTNWTVAAEQTQLEIATQMPGSYCVQVAAVTGAGAGEPSSPVCLILEQAMERAAREPNDHGPWTLEQLRATLRRPEVIASGCIVLWLLLLGTAVCVHHRRRGAVHLGPGERVNWTPDRQASLGPVRTLREVRSLARCWQLQICPSFCPLSGLYRYTSEDAILKHRMDHSDSPWLADTWQSTSGSRDLSSSSSLSSRLGIDPRDPLGCPRSLISWDPRSPGVPLLPDTSTFYGSLITELPSSPPARPSPHAPTVKRLPPQLARLSSPWPSSDSLCSRRGLSSPRLSMAPTEAWKAKKKQELHQANSSPLLRASHPMEHWAWELGKKGSKNLSQSPGAAPRALVAWRALGPQLLNSSSELTRPLPTAPPSSHGVPTQNQPTLRSVELQAPPSHPLPAAPSPILIPSSPKASSLSGPSPASSGLSSSSLSSLGDDQDSVLTPEEVALCLEFSEGEETPRNSVSPMPRAPSPPVTYGYISVPTASELADIDRAGGGVGSEVRGLLCPPRPCLTPTPSEGSLANGWGSASEDNAPSARTSLVSSSDGSFLADAHFARALAVAVDSLGFGLEPKESDCIFTDASSPPSPRDDVFLTPTFSLPLWEWRSDWLEDVESNHTQRLGRGLPPWPPDSQISSQRSQRSCPVSKAGNSS

Target ORF information:

RefSeq Version	XM_016122654.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016122654.1

ORF Insert Sequence:

ATGGGCTCCG GAGGAGCCGG CCTCCTTGGA GCCTGGTGGC CCCTGCCTCT CCTGCTTCTG 
CTCAGCTCAG GAGGCAAGGC TCAGGACTCC CCACCTCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCCGG CCCCGCCAAG ATGAGCTGCC GGGCTTCAGG CCAGCCACCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCTCTGAGTA TGGTGCCCCC AGACGTACAC 
CACCTCCTAC CTGATGGAAC CCTGCTGCTG CTGCGGCCCC CAGCCCAGGG ACGTGCCCAC 
AAGGACCAGG CCCGATCCAC AGACCTAGGC ATCTATACCT GTGAGGCCAG CAACCGGCTG 
GGCACGGCCG TCAGCCGAGG TGCTCGGCTG TCTGTGGCTG TCCTTTGGGA GGATTTCCAG 
ATCCATCCAC GGGACACAGT GGCCACGGTG GGTGAGCAGG TGACCCTGGA GTGTAGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCACA TGGTGGAAGG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGGC GGCACACGGT ATCCAGGGGT TCCTTGCTGA TGATAAGAGC GGAGAAGAGC 
GACGCGGGGA CCTATATGTG CGTGGCCACC AACGACGCGG GACGACGGGA GAGCCGGGCA 
GCCAGGGTGT CGGTCCAGGA GCCCCGGGAC TACAAGGAGC CTCTGGAGAT TCTGGCTATG 
CACATTCGGC TGGAAAACGT GACCTTGATG AACCCAAACC CCACAAAGGG CACCAAGCCT 
GGGCCTGCTG TATGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCGCCTGC TCAGTCCTAC 
ACAGCGCTGT TCAGGGCCCA GACAGCCTCG GGAGGCCAGG GAGCTCCATG GGCAGAGGCC 
CTGCTGGCTG GCTGGCAGAG CGCAGAGCTT GGCGGCCTCC GCTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCCG AACAAGTGCC CAGTGCCCCT CCCCAGGAGG TGACCCTAAA ACTTGGCAAT 
GGCAGTGTCC TTGTGAACTG GGTTCCACCA CCAGCTGAAA ACCACAATGG CGTCATCCGT 
GGCTACCAGG TATGGAGTCT GGGAAACTCC TCGCTCCTCC CAACCAACTG GACAGTGGCT 
GCCGAGCAGA CCCAGCTGGA GATTGCCACC CAAATGCCAG GTTCCTACTG TGTGCAAGTA 
GCTGCAGTCA CTGGTGCTGG GGCTGGGGAG CCCAGTAGCC CCGTTTGCCT CATTTTAGAG 
CAGGCCATGG AGCGAGCTGC CCGAGAACCA AATGACCATG GTCCATGGAC TCTGGAGCAG 
CTGAGAGCTA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGTGTATTGT GCTCTGGCTG 
CTGCTGCTTG GCACTGCAGT GTGTGTCCAC CACCGGCGCC GAGGTGCGGT GCACCTGGGC 
CCAGGTGAGA GGGTAAACTG GACCCCAGAT AGGCAGGCCT CCCTGGGTCC GGTGAGAACG 
CTGAGGGAGG TTCGATCTCT AGCCAGGTGT TGGCAACTCC AGATTTGTCC TTCTTTCTGC 
CCTCTCTCAG GTCTGTACAG ATATACCAGT GAGGATGCCA TCCTAAAACA CAGGATGGAT 
CACAGTGACT CTCCCTGGCT GGCAGACACT TGGCAGTCCA CCTCTGGCTC TCGCGACCTG 
AGCAGCAGCA GTAGCCTCAG CAGCCGGCTA GGAATAGACC CCCGGGATCC ACTAGGCTGT 
CCTCGTTCCT TGATCTCCTG GGATCCCCGC AGCCCCGGAG TGCCCCTGCT TCCCGACACC 
AGCACTTTCT ATGGCTCCCT CATCACTGAA CTGCCTTCCA GCCCTCCAGC CCGGCCGAGC 
CCCCATGCCC CAACTGTCAA GCGTCTCCCA CCCCAGCTGG CCCGGCTCTC CAGCCCCTGG 
CCCAGTTCAG ACAGCCTCTG CAGCCGCAGG GGGCTCTCTT CTCCGCGCTT GTCCATGGCC 
CCCACAGAGG CTTGGAAGGC CAAAAAGAAG CAAGAACTGC ACCAAGCCAA CAGCTCCCCA 
TTGCTCCGGG CCAGCCACCC TATGGAGCAC TGGGCCTGGG AGCTGGGGAA AAAAGGCTCC 
AAGAACCTTT CTCAAAGCCC AGGAGCTGCG CCCCGAGCTC TGGTTGCCTG GCGGGCCCTG 
GGACCACAGC TTCTCAACTC CTCCAGTGAG CTGACTCGCC CTCTCCCCAC AGCACCCCCC 
TCTTCTCATG GAGTCCCCAC TCAGAATCAA CCGACCCTGC GCTCGGTGGA GCTCCAAGCT 
CCCCCCTCCC ACCCACTGCC AGCAGCCCCC AGCCCCATCC TCATCCCCTC CAGTCCCAAG 
GCCTCTTCCC TCTCTGGTCC CAGCCCAGCC TCCAGTGGCC TGTCCAGCTC TTCACTGTCA 
TCTCTGGGGG ACGATCAGGA CAGTGTGCTG ACTCCTGAGG AGGTGGCCCT TTGCCTGGAG 
TTCAGTGAGG GTGAAGAGAC CCCCAGGAAC AGTGTCTCTC CTATGCCAAG GGCTCCTTCG 
CCCCCCGTCA CCTATGGGTA CATCAGCGTC CCGACAGCCT CAGAGCTAGC AGACATAGAC 
AGGGCTGGAG GAGGGGTGGG GTCTGAGGTG AGGGGCTTGT TGTGCCCCCC TCGGCCCTGC 
CTCACCCCTA CTCCCAGTGA GGGCTCCCTG GCCAATGGCT GGGGCTCAGC CTCCGAGGAC 
AATGCCCCCA GCGCCAGAAC CAGCCTCGTC AGCTCTTCTG ATGGCTCCTT CCTCGCGGAT 
GCCCACTTTG CAAGGGCCCT GGCAGTGGCT GTGGACAGCC TTGGCTTTGG TCTGGAGCCC 
AAGGAGTCAG ACTGCATCTT CACAGATGCT TCATCGCCTC CCTCCCCCCG GGATGACGTC 
TTCCTGACCC CTACCTTCTC CCTGCCCCTG TGGGAATGGA GATCAGACTG GCTAGAGGAT 
GTGGAGAGCA ACCACACCCA GCGGCTGGGA AGGGGGCTGC CTCCCTGGCC TCCGGACTCT 
CAGATCTCTT CCCAAAGAAG TCAGCGCAGC TGTCCTGTGT CCAAGGCTGG CAACTCCTCC 
TGA