Clic5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clic5
Entrez Gene ID	94272
Full Name	chloride intracellular channel 5
General protein information	Preferred Names chloride intracellular channel 5 Names chloride intracellular channel protein 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	voltage gated chloride channel that may play a role in kidney function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053603.2	NP_446055.1	chloride intracellular channel protein 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLIC5	XP_001142911.1
Xenopus tropicalis (tropical clawed frog)	clic4	NP_001006831.1
Mus musculus (house mouse)	Clic5	NP_766209.1
Drosophila melanogaster (fruit fly)	Clic	NP_572928.1
Homo sapiens (human)	CLIC5	NP_001107558.1
	CLIC5	XP_001101512.1
Canis lupus familiaris (dog)	CLIC5	XP_538943.2
Bos taurus (cattle)	CLIC5	NP_776701.1
Rattus norvegicus (Norway rat)	Clic5	NP_446055.1
Gallus gallus (chicken)	CLIC5	XP_420060.2
Danio rerio (zebrafish)	clic5a	XP_005169863.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000943	XP_003437145.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Pierchala BA, Mu?oz MR, Tsui CC
Kidney international78(9)868-82(2010 Nov)

The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function.
Gagnon LH, Longo-Guess CM, Berryman M, Shin JB, Saylor KW, Yu H, Gillespie PG, Johnson KR
The Journal of neuroscience : the official journal of the Society for Neuroscience26(40)10188-98(2006 Oct)

Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease.
Steinmeyer K, Schwappach B, Bens M, Vandewalle A, Jentsch TJ
The Journal of biological chemistry270(52)31172-7(1995 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors report the presence of unique dimorphic chloride intracellular channel (CLIC) proteins namely CLIC1, CLIC4 and CLIC5 as abundant CLICs in the rodent heart. Further, CLIC4, CLIC5, and an ortholog present in Drosophila (DmCLIC) localize to adult cardiac mitochondria.
Title: Molecular identity of cardiac mitochondrial chloride intracellular channel proteins.

Study identifies CLIC5 as a new component that is enriched in and necessary for foot process integrity and podocyte function in vivo.
Title: Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.

The following Clic5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clic5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39859	NM_053603.2 Latest version!	Rattus norvegicus chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clic5 ( NM_053603.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446055.1 Rattus norvegicus chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein, mRNA.

Price & Availability↑

CloneID	ORa39859
Clone ID Related Accession (Same CDS sequence)	NM_053603.2
Accession Version	NM_053603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-04-22
Organism	Rattus norvegicus(Norway rat)
Product	chloride intracellular channel protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF323174.1, AABR03068011.1, AABR03071973.1 and AABR03069174.1. On Oct 12, 2007 this sequence version replaced NM_053603.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. COMPLETENESS: complete on the 3' end.

ATGACGGACT CAGCGACAGC TAATGGGGAC GACAGGGACC CCGAGATCGA GCTCTTCGTG 
AAGGCTGGCA TCGATGGAGA GAGCATTGGC AACTGTCCTT TCTCTCAGCG TCTCTTCATG 
ATCCTCTGGC TGAAAGGAGT CGTGTTCAAC GTCACCACCG TGGATCTGAA AAGAAAGCCA 
GCCGATCTAC ACAACCTGGC TCCTGGCACA CACCCGCCCT TCCTGACCTT CAATGGGGAC 
GTGAAGACTG ATGTCAACAA GATTGAGGAG TTCCTGGAGG AGACCTTAAC CCCTGAGAAG 
TACCCCAAAC TGGCTGCAAG GCACAGGGAA TCTAACACTG CGGGCATCGA TATCTTCTCC 
AAGTTTTCAG CCTACATCAA AAACACCAAA CAGCAGAACA ATGCTGCCCT TGAGAGAGGC 
CTGACAAAGG CGCTGAGGAA GCTGGATGAC TACCTGAACA CCCCTCTGCC AGAGGAGATT 
GACACCAACA CCCATGGGGA CGAGAAGGGC TCCCAGCGCA AGTTCCTGGA TGGGGATGAG 
CTAACCCTGG CCGACTGCAA TCTACTGCCC AAACTCCATG TGGTCAAGAT TGTGGCTAAG 
AAGTACAGAA ACTATGACAT CCCAGCTGAG ATGACAGGCT TGTGGCGGTA CCTCAAGAAT 
GCCTATGCAC GGGACGAGTT CACCAACACC TGTGCAGCTG ACAGCGAGAT CGAGTTGGCC 
TACGCAGATG TCGCCAGGCG CCTCAGCCGA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446055.1
CDS	260..1015
Translation	MTDSATANGDDRDPEIELFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAARHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALRKLDDYLNTPLPEEIDTNTHGDEKGSQRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAEMTGLWRYLKNAYARDEFTNTCAADSEIELAYADVARRLSRS

Target ORF information:

RefSeq Version	NM_053603.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053603.2

ORF Insert Sequence:

ATGACGGACT CAGCGACAGC TAATGGGGAC GACAGGGACC CCGAGATCGA GCTCTTCGTG 
AAGGCTGGCA TCGATGGAGA GAGCATTGGC AACTGTCCTT TCTCTCAGCG TCTCTTCATG 
ATCCTCTGGC TGAAAGGAGT CGTGTTCAAC GTCACCACCG TGGATCTGAA AAGAAAGCCA 
GCCGATCTAC ACAACCTGGC TCCTGGCACA CACCCGCCCT TCCTGACCTT CAATGGGGAC 
GTGAAGACTG ATGTCAACAA GATTGAGGAG TTCCTGGAGG AGACCTTAAC CCCTGAGAAG 
TACCCCAAAC TGGCTGCAAG GCACAGGGAA TCTAACACTG CGGGCATCGA TATCTTCTCC 
AAGTTTTCAG CCTACATCAA AAACACCAAA CAGCAGAACA ATGCTGCCCT TGAGAGAGGC 
CTGACAAAGG CGCTGAGGAA GCTGGATGAC TACCTGAACA CCCCTCTGCC AGAGGAGATT 
GACACCAACA CCCATGGGGA CGAGAAGGGC TCCCAGCGCA AGTTCCTGGA TGGGGATGAG 
CTAACCCTGG CCGACTGCAA TCTACTGCCC AAACTCCATG TGGTCAAGAT TGTGGCTAAG 
AAGTACAGAA ACTATGACAT CCCAGCTGAG ATGACAGGCT TGTGGCGGTA CCTCAAGAAT 
GCCTATGCAC GGGACGAGTT CACCAACACC TGTGCAGCTG ACAGCGAGAT CGAGTTGGCC 
TACGCAGATG TCGCCAGGCG CCTCAGCCGA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Kidney international78(9)868-82(2010 Nov)
Pierchala BA,Mu?oz MR,Tsui CC

The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function.
The Journal of neuroscience : the official journal of the Society for Neuroscience26(40)10188-98(2006 Oct)
Gagnon LH,Longo-Guess CM,Berryman M,Shin JB,Saylor KW,Yu H,Gillespie PG,Johnson KR

Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease.
The Journal of biological chemistry270(52)31172-7(1995 Dec)
Steinmeyer K,Schwappach B,Bens M,Vandewalle A,Jentsch TJ