Opn1mw cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Opn1mw
Entrez Gene ID	89810
Full Name	opsin 1 (cone pigments), medium-wave-sensitive
General protein information	Preferred Names opsin 1 (cone pigments), medium-wave-sensitive Names medium-wave-sensitive opsin 1 green cone photoreceptor pigment green-sensitive opsin medium wavelength-sensitive cone opsin opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan), green opsin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37
Summary	a green visual pigment fo the retina; involved in color vision [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053548.1	NP_446000.1	medium-wave-sensitive opsin 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	OPN1LW	NP_776991.1
Gallus gallus (chicken)	OPN1LW	NP_990771.1
Danio rerio (zebrafish)	opn1lw1	NP_571250.1
Pan troglodytes (chimpanzee)	OPN1LW	XP_003317827.1
Rattus norvegicus (Norway rat)	Opn1mw	NP_446000.1
Mus musculus (house mouse)	Opn1mw	NP_032132.1
Danio rerio (zebrafish)	opn1lw2	NP_001002443.1
Homo sapiens (human)	OPN1LW	NP_064445.2
Canis lupus familiaris (dog)	OPN1LW	NP_001184001.1
Xenopus tropicalis (tropical clawed frog)	opn1lw	NP_001096331.1

Gene Ontology
Bibliography

Related articles in PubMed

Hydrogen/Deuterium Exchange Mass Spectrometry of Human Green Opsin Reveals a Conserved Pro-Pro Motif in Extracellular Loop 2 of Monostable Visual G Protein-Coupled Receptors.
Hofmann L, Alexander NS, Sun W, Zhang J, Orban T, Palczewski K
Biochemistry56(17)2338-2348(2017 05)

Dynamics of the rhomboid-like protein RHBDD2 expression in mouse retina and involvement of its human ortholog in retinitis pigmentosa.
Ahmedli NB, Gribanova Y, Njoku CC, Naidu A, Young A, Mendoza E, Yamashita CK, Ozg?l RK, Johnson JE, Fox DA, Farber DB
The Journal of biological chemistry288(14)9742-54(2013 Apr)

Tubby is required for trafficking G protein-coupled receptors to neuronal cilia.
Sun X, Haley J, Bulgakov OV, Cai X, McGinnis J, Li T
Cilia1(1)21(2012 Nov)

G protein-coupled receptors participate in cytokinesis.
Zhang X, Bedigian AV, Wang W, Eggert US
Cytoskeleton (Hoboken, N.J.)69(10)810-8(2012 Oct)

Membrane receptors and transporters involved in the function and transport of vitamin A and its derivatives.
Sun H
Biochimica et biophysica acta1821(1)99-112(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The deficiency in vision of the Opn1mw rat is similar to the color vision defects that occur in humans with a color vision defect but without recessive retinal degeneration.
Title: A novel middle-wavelength opsin (M-opsin) null-mutation in the retinal cone dysfunction rat.

The following Opn1mw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Opn1mw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42563	NM_053548.1 Latest version!	Rattus norvegicus opsin 1, medium wave sensitive (Opn1mw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Opn1mw ( NM_053548.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446000.1 Rattus norvegicus opsin 1, medium wave sensitive (Opn1mw), mRNA.

Price & Availability↑

CloneID	ORa42563
Clone ID Related Accession (Same CDS sequence)	NM_053548.1
Accession Version	NM_053548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	medium-wave-sensitive opsin 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC106169.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAAC AGCTTACAGG TGAACAGACA CTGGACCATT ATGAGGATAG CACCCAGGCA 
AGCATCTTCA CCTATACCAA CAGCAACAGC ACCAGAGGTC CCTTTGAAGG TCCCAATTAT 
CACATTGCTC CAAGGTGGGT GTACCACCTC ACCAGCACCT GGATGATTCT TGTGGTCATT 
GCATCTGTCT TCACAAATGG ACTCGTGCTG GCAGCCACCA TGAGGTTCAA GAAGCTGCGT 
CATCCTCTGA ACTGGATTCT AGTGAACTTG GCAGTTGCTG ACCTAGCAGA GACCATTATT 
GCCAGCACTA TCAGTGTTGT GAACCAAATC TATGGCTACT TTGTGCTGGG CCACCCTCTG 
TGTGTCATAG AAGGCTACAT TGTCTCACTA TGTGGGATCA CAGGCCTCTG GTCCTTGGCC 
ATCATTTCCT GGGAGAGATG GCTGGTGGTC TGCAAGCCCT TTGGCAATGT GAGATTTGAT 
GCTAAACTGG CCACTGTGGG AATCGTCTTC TCCTGGGTCT GGGCTGCTGT ATGGACGGCC 
CCACCAATCT TTGGTTGGAG CAGGTACTGG CCTTATGGCC TGAAGACATC GTGTGGTCCA 
GACGTGTTCA GCGGTACCTC GTATCCTGGG GTTCAGTCTT ATATGATGGT CCTCATGGTC 
ACGTGCTGCA TCTTCCCACT CAGCATCATC GTGCTCTGCT ACCTCCAAGT GTGGCTGGCC 
ATCCGAGCAG TGGCAAAGCA ACAGAAAGAA TCTGAGTCCA CCCAGAAGGC TGAGAAGGAG 
GTGACACGCA TGGTGGTGGT GATGGTCTTC GCATACTGCC TCTGCTGGGG GCCCTATACT 
TTCTTTGCAT GCTTTGCTAC TGCCCATCCT GGCTATGCCT TCCACCCTCT TGTGGCCTCC 
CTACCATCCT ACTTTGCCAA AAGTGCCACT ATCTACAATC CCATTATCTA TGTCTTTATG 
AACCGGCAGT TTCGAAATTG CATCTTACAG CTCTTTGGAA AGAAAGTTGA TGATAGCTCT 
GAACTTTCCA GCACCTCCAA AACAGAAGTC TCATCTGTCT CTTCAGTGTC ACCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446000.1
CDS	1..1080
Translation	MAQQLTGEQTLDHYEDSTQASIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSTWMILVVIASVFTNGLVLAATMRFKKLRHPLNWILVNLAVADLAETIIASTISVVNQIYGYFVLGHPLCVIEGYIVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLATVGIVFSWVWAAVWTAPPIFGWSRYWPYGLKTSCGPDVFSGTSYPGVQSYMMVLMVTCCIFPLSIIVLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMVFAYCLCWGPYTFFACFATAHPGYAFHPLVASLPSYFAKSATIYNPIIYVFMNRQFRNCILQLFGKKVDDSSELSSTSKTEVSSVSSVSPA

Target ORF information:

RefSeq Version	NM_053548.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus opsin 1, medium wave sensitive (Opn1mw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053548.1

ORF Insert Sequence:

ATGGCCCAAC AGCTTACAGG TGAACAGACA CTGGACCATT ATGAGGATAG CACCCAGGCA 
AGCATCTTCA CCTATACCAA CAGCAACAGC ACCAGAGGTC CCTTTGAAGG TCCCAATTAT 
CACATTGCTC CAAGGTGGGT GTACCACCTC ACCAGCACCT GGATGATTCT TGTGGTCATT 
GCATCTGTCT TCACAAATGG ACTCGTGCTG GCAGCCACCA TGAGGTTCAA GAAGCTGCGT 
CATCCTCTGA ACTGGATTCT AGTGAACTTG GCAGTTGCTG ACCTAGCAGA GACCATTATT 
GCCAGCACTA TCAGTGTTGT GAACCAAATC TATGGCTACT TTGTGCTGGG CCACCCTCTG 
TGTGTCATAG AAGGCTACAT TGTCTCACTA TGTGGGATCA CAGGCCTCTG GTCCTTGGCC 
ATCATTTCCT GGGAGAGATG GCTGGTGGTC TGCAAGCCCT TTGGCAATGT GAGATTTGAT 
GCTAAACTGG CCACTGTGGG AATCGTCTTC TCCTGGGTCT GGGCTGCTGT ATGGACGGCC 
CCACCAATCT TTGGTTGGAG CAGGTACTGG CCTTATGGCC TGAAGACATC GTGTGGTCCA 
GACGTGTTCA GCGGTACCTC GTATCCTGGG GTTCAGTCTT ATATGATGGT CCTCATGGTC 
ACGTGCTGCA TCTTCCCACT CAGCATCATC GTGCTCTGCT ACCTCCAAGT GTGGCTGGCC 
ATCCGAGCAG TGGCAAAGCA ACAGAAAGAA TCTGAGTCCA CCCAGAAGGC TGAGAAGGAG 
GTGACACGCA TGGTGGTGGT GATGGTCTTC GCATACTGCC TCTGCTGGGG GCCCTATACT 
TTCTTTGCAT GCTTTGCTAC TGCCCATCCT GGCTATGCCT TCCACCCTCT TGTGGCCTCC 
CTACCATCCT ACTTTGCCAA AAGTGCCACT ATCTACAATC CCATTATCTA TGTCTTTATG 
AACCGGCAGT TTCGAAATTG CATCTTACAG CTCTTTGGAA AGAAAGTTGA TGATAGCTCT 
GAACTTTCCA GCACCTCCAA AACAGAAGTC TCATCTGTCT CTTCAGTGTC ACCTGCATAA