Vamp7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vamp7
Entrez Gene ID	85491
Full Name	vesicle-associated membrane protein 7
Synonyms	Sybl1
General protein information	Preferred Names vesicle-associated membrane protein 7 Names vesicle-associated membrane protein 7 VAMP-7 synaptobrevin-like 1 synaptobrevin-like protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11
Summary	member of the SNARE family of proteins; displays insensitivity to tetanus toxin; may play a role in degranulation of basophilic leukemia cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053531.1	NP_445983.1	vesicle-associated membrane protein 7
XM_006249016.3	XP_006249078.1	vesicle-associated membrane protein 7 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	VAMP7	NP_005629.1
Pan troglodytes (chimpanzee)	VAMP7	XP_003317850.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000700	XP_003437068.1
Mus musculus (house mouse)	Vamp7	NP_035645.1
Rattus norvegicus (Norway rat)	Vamp7	NP_445983.1
Gallus gallus (chicken)	VAMP7	NP_001026292.1
Drosophila melanogaster (fruit fly)	CG1599	NP_610524.1
Oryza sativa (rice)	Os10g0154000	NP_001064184.1
Canis lupus familiaris (dog)	VAMP7	XP_855489.3
Bos taurus (cattle)	VAMP7	NP_001069770.1
Danio rerio (zebrafish)	sybl1	NP_956560.1
Arabidopsis thaliana (thale cress)	VAMP714	NP_197628.1
Xenopus tropicalis (tropical clawed frog)	vamp7	NP_001135622.1

Gene Ontology
Bibliography

Related articles in PubMed

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D, Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M, Liang C
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)

Reelin mobilizes a VAMP7-dependent synaptic vesicle pool and selectively augments spontaneous neurotransmission.
Bal M, Leitz J, Reese AL, Ramirez DM, Durakoglugil M, Herz J, Monteggia LM, Kavalali ET
Neuron80(4)934-46(2013 Nov)

The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Itakura E, Kishi-Itakura C, Mizushima N
Cell151(6)1256-69(2012 Dec)

A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery.
Burgo A, Proux-Gillardeaux V, Sotirakis E, Bun P, Casano A, Verraes A, Liem RK, Formstecher E, Coppey-Moisan M, Galli T
Developmental cell23(1)166-80(2012 Jul)

Granule exocytosis is required for platelet spreading: differential sorting of ?-granules expressing VAMP-7.
Peters CG, Michelson AD, Flaumenhaft R
Blood120(1)199-206(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The specificity of Reelin action on spontaneous neurotransmitter release requires VAMP7
Title: Reelin mobilizes a VAMP7-dependent synaptic vesicle pool and selectively augments spontaneous neurotransmission.

Downregulation of VAMP7 expression inhibited the fusion of ATP-storing vesicles and ATP-mediated calcium wave propagation.
Title: TI-VAMP/VAMP7 is the SNARE of secretory lysosomes contributing to ATP secretion from astrocytes.

VAMP2, VAMP5, and VAMP7 may be involved in translocation of GLUT4 during muscle contractions.
Title: Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.

show that TI-VAMP interacts with the Vps9 domain and ankyrin-repeat-containing protein Varp, a guanine nucleotide exchange factor of the small GTPase Rab21, through a specific domain herein called the interacting domain.
Title: Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth.

The following Vamp7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vamp7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09815	NM_053531.1 Latest version!	Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa34689	XM_006249016.3 Latest version!	Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vamp7 ( NM_053531.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445983.1 Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), mRNA.

Price & Availability↑

CloneID	ORa09815
Clone ID Related Accession (Same CDS sequence)	NM_053531.1
Accession Version	NM_053531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-associated membrane protein 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF281632.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF281632.1, CV080368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCATTC TTTTTGCCGT TGTTGCCAGG GGAACCACTA TTCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCTCA TGGCAATTAT TTGTTTCACT ACATCTGCCA GGACAGGATT 
GTGTATCTTT GCATCACGGA TGACGATTTC GAGCGTTCTC GGGCCTTCGG TTTTTTGAAT 
GAAGTGAAGA AGAGGTTCCA GACCACATAT GGCTCGAGAG CGCAGACCGC ACTTCCCTAT 
GCCATGAACA GTGAGTTTTC GAGCGTTCTG GCTGCACAAC TGAAGCATCA CTCCGAGAAT 
CAGAGCCTGG ACAGAGTGAC GGAGACTCAA GCCCAAGTGG ACGAACTGAA AGGCATCATG 
GTCAGAAACA TAGATCTAGT TGCTCAGCGT GGAGAAAGGC TAGAATTGCT GATAGATAAA 
ACAGAAAACC TCGTAGATTC GTCCGTCACC TTCAAAACGA CCAGCAGGAA CCTTGCCCGG 
GCCATGTGCG TGAAGAACGT CAAGCTCACT GCCATCATCG TCGTTGTATC GATTGTGTTC 
ATCTACATCA TCGTGTCTCC ACTGTGCGGT GGCTTCACGT GGCCAAGCTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445983.1
CDS	1..663
Translation	MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDRIVYLCITDDDFERSRAFGFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENQSLDRVTETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCVKNVKLTAIIVVVSIVFIYIIVSPLCGGFTWPSCVKK

Target ORF information:

RefSeq Version	NM_053531.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053531.1

ORF Insert Sequence:

ATGGCCATTC TTTTTGCCGT TGTTGCCAGG GGAACCACTA TTCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCTCA TGGCAATTAT TTGTTTCACT ACATCTGCCA GGACAGGATT 
GTGTATCTTT GCATCACGGA TGACGATTTC GAGCGTTCTC GGGCCTTCGG TTTTTTGAAT 
GAAGTGAAGA AGAGGTTCCA GACCACATAT GGCTCGAGAG CGCAGACCGC ACTTCCCTAT 
GCCATGAACA GTGAGTTTTC GAGCGTTCTG GCTGCACAAC TGAAGCATCA CTCCGAGAAT 
CAGAGCCTGG ACAGAGTGAC GGAGACTCAA GCCCAAGTGG ACGAACTGAA AGGCATCATG 
GTCAGAAACA TAGATCTAGT TGCTCAGCGT GGAGAAAGGC TAGAATTGCT GATAGATAAA 
ACAGAAAACC TCGTAGATTC GTCCGTCACC TTCAAAACGA CCAGCAGGAA CCTTGCCCGG 
GCCATGTGCG TGAAGAACGT CAAGCTCACT GCCATCATCG TCGTTGTATC GATTGTGTTC 
ATCTACATCA TCGTGTCTCC ACTGTGCGGT GGCTTCACGT GGCCAAGCTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( XM_006249016.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249078.1 Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34689
Clone ID Related Accession (Same CDS sequence)	XM_006249016.3
Accession Version	XM_006249016.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-associated membrane protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTC TTTTTGCCGT TGTTGCCAGG GGAACCACTA TTCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCTCA TGGCAATTAT TTGTTTCACT ACATCTGCCA GGACAGGATT 
GTGTATCTTT GCATCACGGA TGACGATTTC GAGCGTTCTC GGGCCTTCGG TTTTTTGAAT 
GAAGTGAAGA AGAGGTTCCA GACCACATAT GGCTCGAGAG CACAGACCGC ACTTCCCTAT 
GCCATGAACA GTGAGTTTTC GAGCGTTCTG GCTGCACAAC TGAAGCATCA CTCCGAGAAT 
CAGAGCCTGG ACAGAGTGAC GGAGACTCAA GCCCAAGTGG ACGAACTGAA AGGCATCATG 
GTCAGAAACA TAGATCTAGT TGCTCAGCGT GGAGAAAGGC TAGAATTGCT GATAGATAAA 
ACAGAAAACC TCGTAGATTC GTCCGTCACC TTCAAGACGA CCAGCAGGAA CCTTGCCCGG 
GCCATGTGCG TGAAGAACGT CAAGCTCACT GCCATCATCG TCGTTGTATC GATTGTGTTC 
ATCTACATCA TCGTGTCTCC ACTGTGCGGT GGCTTCACGT GGCCAAGCTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249078.1
CDS	154..816
Translation	MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDRIVYLCITDDDFERSRAFGFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENQSLDRVTETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCVKNVKLTAIIVVVSIVFIYIIVSPLCGGFTWPSCVKK

Target ORF information:

RefSeq Version	XM_006249016.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249016.3

ORF Insert Sequence:

ATGGCCATTC TTTTTGCCGT TGTTGCCAGG GGAACCACTA TTCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCTCA TGGCAATTAT TTGTTTCACT ACATCTGCCA GGACAGGATT 
GTGTATCTTT GCATCACGGA TGACGATTTC GAGCGTTCTC GGGCCTTCGG TTTTTTGAAT 
GAAGTGAAGA AGAGGTTCCA GACCACATAT GGCTCGAGAG CACAGACCGC ACTTCCCTAT 
GCCATGAACA GTGAGTTTTC GAGCGTTCTG GCTGCACAAC TGAAGCATCA CTCCGAGAAT 
CAGAGCCTGG ACAGAGTGAC GGAGACTCAA GCCCAAGTGG ACGAACTGAA AGGCATCATG 
GTCAGAAACA TAGATCTAGT TGCTCAGCGT GGAGAAAGGC TAGAATTGCT GATAGATAAA 
ACAGAAAACC TCGTAGATTC GTCCGTCACC TTCAAGACGA CCAGCAGGAA CCTTGCCCGG 
GCCATGTGCG TGAAGAACGT CAAGCTCACT GCCATCATCG TCGTTGTATC GATTGTGTTC 
ATCTACATCA TCGTGTCTCC ACTGTGCGGT GGCTTCACGT GGCCAAGCTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)
Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

Reelin mobilizes a VAMP7-dependent synaptic vesicle pool and selectively augments spontaneous neurotransmission.
Neuron80(4)934-46(2013 Nov)
Bal M,Leitz J,Reese AL,Ramirez DM,Durakoglugil M,Herz J,Monteggia LM,Kavalali ET

The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Cell151(6)1256-69(2012 Dec)
Itakura E,Kishi-Itakura C,Mizushima N

A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery.
Developmental cell23(1)166-80(2012 Jul)
Burgo A,Proux-Gillardeaux V,Sotirakis E,Bun P,Casano A,Verraes A,Liem RK,Formstecher E,Coppey-Moisan M,Galli T

Granule exocytosis is required for platelet spreading: differential sorting of ?-granules expressing VAMP-7.
Blood120(1)199-206(2012 Jul)
Peters CG,Michelson AD,Flaumenhaft R