Nol3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nol3
Entrez Gene ID	85383
Full Name	nucleolar protein 3
Synonyms	Arc
General protein information	Preferred Names nucleolar protein 3 Names nucleolar protein 3 apoptosis repressor with CARD nucleolar protein 3 (apoptosis repressor with CARD domain)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	plays a role in regulation of apoptosis; decreased expression may contribute to hypoxia-induced neuronal cell death [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053516.2	NP_445968.2	nucleolar protein 3
XM_006255539.3	XP_006255601.1	nucleolar protein 3 isoform X1
XM_017601394.1	XP_017456883.1	nucleolar protein 3 isoform X1
XM_006255542.3	XP_006255604.1	nucleolar protein 3 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nol3	NP_084428.1
	NOL3	XP_001087572.1
Homo sapiens (human)	NOL3	NP_001263241.1
Canis lupus familiaris (dog)	NOL3	XP_005620912.1
Bos taurus (cattle)	NOL3	NP_001077157.1
Rattus norvegicus (Norway rat)	Nol3	NP_445968.2

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of ARC promoting the apoptosis of rat pulmonary arterial smooth muscle cells after serum deprivation in?vitro.
Zhang J, Wu Z, Guan M, Lu H, Mo X
Molecular medicine reports16(4)3869-3876(2017 Oct)

A novel role for the apoptosis inhibitor ARC in suppressing TNF?-induced regulated necrosis.
Kung G, Dai P, Deng L, Kitsis RN
Cell death and differentiation21(4)634-44(2014 Apr)

Transcription factor Foxo3a prevents apoptosis by regulating calcium through the apoptosis repressor with caspase recruitment domain.
Lu D, Liu J, Jiao J, Long B, Li Q, Tan W, Li P
The Journal of biological chemistry288(12)8491-504(2013 Mar)

Apoptosis repressor with a CARD domain (ARC) restrains Bax-mediated pathogenesis in dystrophic skeletal muscle.
Davis J, Kwong JQ, Kitsis RN, Molkentin JD
PloS one8(12)e82053(2013)

Low-dose spironolactone prevents apoptosis repressor with caspase recruitment domain degradation during myocardial infarction.
Loan Le TY, Mardini M, Howell VM, Funder JW, Ashton AW, Mihailidou AS
Hypertension (Dallas, Tex. : 1979)59(6)1164-9(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Foxo3a maintains calcium homeostasis and inhibits cardiac apoptosis through trans-activation of the ARC promoter.
Title: Transcription factor Foxo3a prevents apoptosis by regulating calcium through the apoptosis repressor with caspase recruitment domain.

Data show that low-dose mineralocorticoid receptor antagonists reduce infarct size and apoptosis in the reperfused myocardium by preventing the apoptosis repressor with caspase recruitment domain (ARC) degradation.
Title: Low-dose spironolactone prevents apoptosis repressor with caspase recruitment domain degradation during myocardial infarction.

A novel cardiac pathway is identified in which anesthetic preconditioning results in increased phosphorylation of nucleolar protein 3 following inhibition of calcineurin increase.
Title: Phosphorylation of ARC is a critical element in the antiapoptotic effect of anesthetic preconditioning.

ARC protein is expressed in vascular smooth muscle and is significantly reduced in several target tissues during hypertension.
Title: Apoptosis repressor with caspase recruitment domain is dramatically reduced in cardiac, skeletal, and vascular smooth muscle during hypertension.

Studies provide evidence for the protective role of anti-apoptotic ARC in Dox-induced cardiotoxicity, which makes this molecule an interesting target for future therapies.
Title: ARC is a critical cardiomyocyte survival switch in doxorubicin cardiotoxicity.

The following Nol3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nol3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12997	NM_053516.2 Latest version!	Rattus norvegicus nucleolar protein 3 (Nol3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
ORa38891	XM_017601394.1 Latest version!	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa38891	XM_006255539.3 Latest version!	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa38892	XM_006255542.3 Latest version!	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nol3 ( NM_053516.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445968.2 Rattus norvegicus nucleolar protein 3 (Nol3), mRNA.

Price & Availability↑

CloneID	ORa12997
Clone ID Related Accession (Same CDS sequence)	NM_053516.2
Accession Version	NM_053516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	nucleolar protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U40627.1. On Jul 24, 2004 this sequence version replaced NM_053516.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40627.1, CB782636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGCGAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445968.2
CDS	86..751
Translation	MGNMQERPSETIDRERKRLVETLQADSGLLLDALVARGVLTGPEYEALDALPDAERRVRRLLLLVQSKGEAACQELLRCAQQTVSMPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPSSGTTCPGLPRASEEEEIGGPEDSEAVQPRTPEEPELEAEATKGDEPDLEQEMEPEPEPEVEPEPEPEPEPEPEPEPEPEPEPEPEREPDFQEGDESEGCENT

Target ORF information:

RefSeq Version	NM_053516.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleolar protein 3 (Nol3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053516.2

ORF Insert Sequence:

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGCGAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol3 ( XM_006255539.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255601.1 Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38891
Clone ID Related Accession (Same CDS sequence)	XM_006255539.3 , XM_017601394.1
Accession Version	XM_006255539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleolar protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255601.1
CDS	132..797
Translation	MGNMQERPSETIDRERKRLVETLQADSGLLLDALVARGVLTGPEYEALDALPDAERRVRRLLLLVQSKGEAACQELLRCAQQTVSMPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPSSGTTCPGLPRASEEEEIGGPEDSEAVQPRTPEEPELEAEATKGDEPDLEQEMEPEPEPEVEPEPEPEPEPEPEPEPEPEPEPEPEAEPDFQEGDESEGCENT

Target ORF information:

RefSeq Version	XM_006255539.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255539.3

ORF Insert Sequence:

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol3 ( XM_017601394.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456883.1 Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38891
Clone ID Related Accession (Same CDS sequence)	XM_006255539.3 , XM_017601394.1
Accession Version	XM_017601394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleolar protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456883.1
CDS	200..865
Translation	MGNMQERPSETIDRERKRLVETLQADSGLLLDALVARGVLTGPEYEALDALPDAERRVRRLLLLVQSKGEAACQELLRCAQQTVSMPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPSSGTTCPGLPRASEEEEIGGPEDSEAVQPRTPEEPELEAEATKGDEPDLEQEMEPEPEPEVEPEPEPEPEPEPEPEPEPEPEPEPEAEPDFQEGDESEGCENT

Target ORF information:

RefSeq Version	XM_017601394.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601394.1

ORF Insert Sequence:

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGG TTGTGAGAAC 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nol3 ( XM_006255542.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255604.1 Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38892
Clone ID Related Accession (Same CDS sequence)	XM_006255542.3
Accession Version	XM_006255542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleolar protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGA TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255604.1
CDS	132..788
Translation	MGNMQERPSETIDRERKRLVETLQADSGLLLDALVARGVLTGPEYEALDALPDAERRVRRLLLLVQSKGEAACQELLRCAQQTVSMPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPSSGTTCPGLPRASEEEEIGGPEDSEAVQPRTPEEPELEAEATKGDEPDLEQEMEPEPEPEVEPEPEPEPEPEPEPEPEPEPEPEPEAEPDFQEGDESEDS

Target ORF information:

RefSeq Version	XM_006255542.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleolar protein 3 (Nol3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255542.3

ORF Insert Sequence:

ATGGGTAACA TGCAGGAGCG CCCATCGGAG ACCATTGACC GGGAACGGAA ACGGCTGGTA 
GAAACATTGC AGGCTGACTC TGGGTTGCTG CTGGACGCGC TGGTGGCCCG GGGCGTCCTC 
ACTGGGCCTG AGTATGAAGC CTTGGATGCG CTGCCTGATG CAGAGCGCAG GGTGCGCCGC 
CTACTGCTGT TGGTGCAGAG CAAGGGCGAG GCAGCCTGCC AGGAACTACT GCGCTGTGCC 
CAGCAAACTG TGAGCATGCC AGACCCGGCC TGGGATTGGC AGCACGTTGG GCCTGGCTAC 
CGGGACCGCA GCTATGACCC TCCATGCCCA GGCCACTGGA CGCCAGAGGC ACCCAGTTCA 
GGGACCACAT GTCCTGGGCT GCCCAGAGCT TCAGAGGAAG AGGAGATCGG AGGTCCTGAG 
GACTCTGAGG CAGTACAGCC TCGAACACCA GAGGAGCCAG AACTAGAAGC TGAAGCTACT 
AAAGGGGATG AGCCAGACCT GGAACAAGAA ATGGAACCAG AACCAGAGCC AGAGGTAGAA 
CCGGAGCCAG AGCCGGAGCC GGAGCCGGAG CCGGAGCCGG AGCCGGAGCC GGAGCCGGAG 
CCGGAGCCAG AGGCAGAGCC TGACTTCCAA GAAGGGGATG AGTCTGAAGA TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of ARC promoting the apoptosis of rat pulmonary arterial smooth muscle cells after serum deprivation in?vitro.
Molecular medicine reports16(4)3869-3876(2017 Oct)
Zhang J,Wu Z,Guan M,Lu H,Mo X

A novel role for the apoptosis inhibitor ARC in suppressing TNF?-induced regulated necrosis.
Cell death and differentiation21(4)634-44(2014 Apr)
Kung G,Dai P,Deng L,Kitsis RN

Transcription factor Foxo3a prevents apoptosis by regulating calcium through the apoptosis repressor with caspase recruitment domain.
The Journal of biological chemistry288(12)8491-504(2013 Mar)
Lu D,Liu J,Jiao J,Long B,Li Q,Tan W,Li P

Apoptosis repressor with a CARD domain (ARC) restrains Bax-mediated pathogenesis in dystrophic skeletal muscle.
PloS one8(12)e82053(2013)
Davis J,Kwong JQ,Kitsis RN,Molkentin JD

Low-dose spironolactone prevents apoptosis repressor with caspase recruitment domain degradation during myocardial infarction.
Hypertension (Dallas, Tex. : 1979)59(6)1164-9(2012 Jun)
Loan Le TY,Mardini M,Howell VM,Funder JW,Ashton AW,Mihailidou AS