Nfs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfs1
Entrez Gene ID	84594
Full Name	NFS1 cysteine desulfurase
Synonyms	Nifs
General protein information	Preferred Names NFS1 cysteine desulfurase Names cysteine desulfurase, mitochondrial NFS1 nitrogen fixation 1 homolog cysteine desulfurase, mitochondrial-like nitrogen fixation gene 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	a putative cysteine desulfurase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053462.2	NP_445914.2	cysteine desulfurase, mitochondrial
XM_006235365.2	XP_006235427.1	cysteine desulfurase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NFS1	NP_066923.3
Drosophila melanogaster (fruit fly)	CG12264	NP_609533.1
Kluyveromyces lactis	KLLA0D04928g	XP_453279.1
Oryza sativa (rice)	Os09g0338400	NP_001062914.1
Xenopus tropicalis (tropical clawed frog)	nfs1	XP_002932937.2
Pan troglodytes (chimpanzee)	NFS1	XP_001165456.1
	NFS1	XP_001097983.1
Mus musculus (house mouse)	Nfs1	NP_035041.2
Rattus norvegicus (Norway rat)	Nfs1	NP_445914.2
Rattus norvegicus (Norway rat)	LOC100911034	XP_003749657.1
Gallus gallus (chicken)	NFS1	NP_001026018.1
Saccharomyces cerevisiae (baker's yeast)	NFS1	NP_009912.2
Eremothecium gossypii	AGOS_AAL013W	NP_982529.1
Arabidopsis thaliana (thale cress)	NFS1	NP_201373.1
Bos taurus (cattle)	NFS1	NP_001092471.1
Gallus gallus (chicken)	LOC100858210	XP_003642486.1
Caenorhabditis elegans (roundworm)	B0205.6	NP_492812.2
Schizosaccharomyces pombe (fission yeast)	nfs1	NP_596002.2
Magnaporthe oryzae (rice blast fungus)	MGG_17371	XP_003718229.1
Neurospora crassa	NCU04636	XP_959179.1
Canis lupus familiaris (dog)	NFS1	XP_534405.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009094	XP_319845.3
Danio rerio (zebrafish)	nfs1	NP_001038458.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial Hspa9/Mortalin regulates erythroid differentiation via iron-sulfur cluster assembly.
Shan Y, Cortopassi G
Mitochondrion2694-103(2016 Jan)

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Maio N, Rouault TA
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)

Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex.
Tsai CL, Barondeau DP
Biochemistry49(43)9132-9(2010 Nov)

RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells.
Fosset C, Chauveau MJ, Guillon B, Canal F, Drapier JC, Bouton C
The Journal of biological chemistry281(35)25398-406(2006 Sep)

Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation.
Biederbick A, Stehling O, R?sser R, Niggemeyer B, Nakai Y, Els?sser HP, Lill R
Molecular and cellular biology26(15)5675-87(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nfs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12481	NM_053462.2 Latest version!	Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa26573	XM_006235365.2 Latest version!	Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfs1 ( NM_053462.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445914.2 Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA.

Price & Availability↑

CloneID	ORa12481
Clone ID Related Accession (Same CDS sequence)	NM_053462.2
Accession Version	NM_053462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cysteine desulfurase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104699.1. On Oct 4, 2005 this sequence version replaced NM_053462.1. ##Evidence-Data-START## Transcript exon combination :: BC104699.1, BC089205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG CAGGTAGCGT CTCTGATTGG AGCGGATCCT 
CGGGAGATCA TTTTCACTAG TGGAGCTACG GAATCCAACA ACATAGCAAT TAAGGGAGTG 
GCCAGGTTCT ACAGGTCACG GAAAAAGCAC TTGGTCACCA CGCAGACAGA ACACAAGTGC 
GTGCTGGATT CGTGCCGCTC CCTGGAAGCT GAGGGCTTTC GGGTCACTTA CCTCCCCGTT 
CAGAAGAGTG GGATCATTGA CTTAAAGGAA CTAGAGGCTG CCATCCAGCC AGATACCAGC 
CTGGTCTCCG TTATGACTGT AAACAATGAG ATTGGAGTAA AGCAACCGAT TGCAGAAATA 
GGCCAGATTT GCAGTTCCAG AAAGCTGTAC TTCCATACTG ATGCAGCCCA AGCTGTTGGG 
AAAATCCCAC TTGATGTCAA TGACATGAAA ATTGATCTCA TGAGTATCAG TGGTCACAAA 
CTCTACGGCC CTAAAGGGGT TGGTGCTATC TATATCCGCC GTAGGCCCCG GGTGCGTGTG 
GAGGCCCTAC AAAGTGGAGG CGGGCAGGAG CGGGGTATGC GGTCTGGGAC AGTGCCCACA 
CCCTTGGTGG TGGGCCTGGG AGCTGCATGT GAGCTGGCAC AACAAGAGAT GGAGTACGAC 
CATAAACGGA TCTCAAAGTT AGCAGAGCGA CTCATACAGA AGATAATGAA GAACCTTCCA 
GATGTGGTGA TGAACGGGGA CCCGAAGCAG CACTACCCTG GCTGTATCAA CCTCTCCTTT 
GCGTATGTGG AAGGGGAAAG CCTGCTGATG GCACTCAAGG ACGTTGCCTT GTCCTCGGGG 
AGTGCCTGCA CTTCCGCATC ATTGGAGCCC TCGTATGTCC TCAGAGCCAT CGGTACGGAT 
GAGGATTTAG CACACTCCTC TATCAGGTTC GGCATCGGCC GCTTCACTAC AGAGGAGGAG 
GTAGACTACA CCGTGCAGAA GTGCATCCAC CATGTGAAAC GTCTTCGAGA AATGAGTCCT 
CTCTGGGAGA TGGTGCAGGA TGGCATTGAC CTCAAGAGCA TCAAGTGGAC CCAGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445914.2
CDS	16..1395
Translation	MVGSVAGNMLLRAAWRRASLAATSVALRRSSVPTRGLRLRVVDHAPHSAVPSEAEAVLRPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH

Target ORF information:

RefSeq Version	NM_053462.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053462.2

ORF Insert Sequence:

ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG CAGGTAGCGT CTCTGATTGG AGCGGATCCT 
CGGGAGATCA TTTTCACTAG TGGAGCTACG GAATCCAACA ACATAGCAAT TAAGGGAGTG 
GCCAGGTTCT ACAGGTCACG GAAAAAGCAC TTGGTCACCA CGCAGACAGA ACACAAGTGC 
GTGCTGGATT CGTGCCGCTC CCTGGAAGCT GAGGGCTTTC GGGTCACTTA CCTCCCCGTT 
CAGAAGAGTG GGATCATTGA CTTAAAGGAA CTAGAGGCTG CCATCCAGCC AGATACCAGC 
CTGGTCTCCG TTATGACTGT AAACAATGAG ATTGGAGTAA AGCAACCGAT TGCAGAAATA 
GGCCAGATTT GCAGTTCCAG AAAGCTGTAC TTCCATACTG ATGCAGCCCA AGCTGTTGGG 
AAAATCCCAC TTGATGTCAA TGACATGAAA ATTGATCTCA TGAGTATCAG TGGTCACAAA 
CTCTACGGCC CTAAAGGGGT TGGTGCTATC TATATCCGCC GTAGGCCCCG GGTGCGTGTG 
GAGGCCCTAC AAAGTGGAGG CGGGCAGGAG CGGGGTATGC GGTCTGGGAC AGTGCCCACA 
CCCTTGGTGG TGGGCCTGGG AGCTGCATGT GAGCTGGCAC AACAAGAGAT GGAGTACGAC 
CATAAACGGA TCTCAAAGTT AGCAGAGCGA CTCATACAGA AGATAATGAA GAACCTTCCA 
GATGTGGTGA TGAACGGGGA CCCGAAGCAG CACTACCCTG GCTGTATCAA CCTCTCCTTT 
GCGTATGTGG AAGGGGAAAG CCTGCTGATG GCACTCAAGG ACGTTGCCTT GTCCTCGGGG 
AGTGCCTGCA CTTCCGCATC ATTGGAGCCC TCGTATGTCC TCAGAGCCAT CGGTACGGAT 
GAGGATTTAG CACACTCCTC TATCAGGTTC GGCATCGGCC GCTTCACTAC AGAGGAGGAG 
GTAGACTACA CCGTGCAGAA GTGCATCCAC CATGTGAAAC GTCTTCGAGA AATGAGTCCT 
CTCTGGGAGA TGGTGCAGGA TGGCATTGAC CTCAAGAGCA TCAAGTGGAC CCAGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfs1 ( XM_006235365.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235427.1 Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26573
Clone ID Related Accession (Same CDS sequence)	XM_006235365.2
Accession Version	XM_006235365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cysteine desulfurase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006235365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG GTAGCGTCTC TGATTGGAGC GGATCCTCGG 
GAGATCATTT TCACTAGTGG AGCTACGGAA TCCAACAACA TAGCAATTAA GGGAGTGGCC 
AGGTTCTACA GGTCACGGAA AAAGCACTTG GTCACCACGC AGACAGAACA CAAGTGCGTG 
CTGGATTCGT GCCGCTCCCT GGAAGCTGAG GGCTTTCGGG TCACTTACCT CCCCGTTCAG 
AAGAGTGGGA TCATTGACTT AAAGGAACTA GAGGCTGCCA TCCAGCCAGA TACCAGCCTG 
GTCTCCGTTA TGACTGTAAA CAATGAGATT GGAGTAAAGC AACCGATTGC AGAAATAGGC 
CAGATTTGCA GTTCCAGAAA GCTGTACTTC CATACTGATG CAGCCCAAGC TGTTGGGAAA 
ATCCCACTTG ATGTCAATGA CATGAAAATT GATCTCATGA GTATCAGTGG TCACAAACTC 
TACGGCCCTA AAGGGGTTGG TGCTATCTAT ATCCGCCGTA GGCCCCGGGT GCGTGTGGAG 
GCCCTACAAA GTGGAGGCGG GCAGGAGCGG GGTATGCGGT CTGGGACAGT GCCCACACCC 
TTGGTGGTGG GCCTGGGAGC TGCATGTGAG CTGGCACAAC AAGAGATGGA GTACGACCAT 
AAACGGATCT CAAAGTTAGC AGAGCGACTC ATACAGAAGA TAATGAAGAA CCTTCCAGAT 
GTGGTGATGA ACGGGGACCC GAAGCAGCAC TACCCTGGCT GTATCAACCT CTCCTTTGCG 
TATGTGGAAG GGGAAAGCCT GCTGATGGCA CTCAAGGACG TTGCCTTGTC CTCGGGGAGT 
GCCTGCACTT CCGCATCATT GGAGCCCTCG TATGTCCTCA GAGCCATCGG TACGGATGAG 
GATTTAGCAC ACTCCTCTAT CAGGTTCGGC ATCGGCCGCT TCACTACAGA GGAGGAGGTA 
GACTACACCG TGCAGAAGTG CATCCACCAT GTGAAACGTC TTCGAGAAAT GAGTCCTCTC 
TGGGAGATGG TGCAGGATGG CATTGACCTC AAGAGCATCA AGTGGACCCA GCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235427.1
CDS	18..1394
Translation	MVGSVAGNMLLRAAWRRASLAATSVALRRSSVPTRGLRLRVVDHAPHSAVPSEAEAVLRPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH

Target ORF information:

RefSeq Version	XM_006235365.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235365.2

ORF Insert Sequence:

ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG GTAGCGTCTC TGATTGGAGC GGATCCTCGG 
GAGATCATTT TCACTAGTGG AGCTACGGAA TCCAACAACA TAGCAATTAA GGGAGTGGCC 
AGGTTCTACA GGTCACGGAA AAAGCACTTG GTCACCACGC AGACAGAACA CAAGTGCGTG 
CTGGATTCGT GCCGCTCCCT GGAAGCTGAG GGCTTTCGGG TCACTTACCT CCCCGTTCAG 
AAGAGTGGGA TCATTGACTT AAAGGAACTA GAGGCTGCCA TCCAGCCAGA TACCAGCCTG 
GTCTCCGTTA TGACTGTAAA CAATGAGATT GGAGTAAAGC AACCGATTGC AGAAATAGGC 
CAGATTTGCA GTTCCAGAAA GCTGTACTTC CATACTGATG CAGCCCAAGC TGTTGGGAAA 
ATCCCACTTG ATGTCAATGA CATGAAAATT GATCTCATGA GTATCAGTGG TCACAAACTC 
TACGGCCCTA AAGGGGTTGG TGCTATCTAT ATCCGCCGTA GGCCCCGGGT GCGTGTGGAG 
GCCCTACAAA GTGGAGGCGG GCAGGAGCGG GGTATGCGGT CTGGGACAGT GCCCACACCC 
TTGGTGGTGG GCCTGGGAGC TGCATGTGAG CTGGCACAAC AAGAGATGGA GTACGACCAT 
AAACGGATCT CAAAGTTAGC AGAGCGACTC ATACAGAAGA TAATGAAGAA CCTTCCAGAT 
GTGGTGATGA ACGGGGACCC GAAGCAGCAC TACCCTGGCT GTATCAACCT CTCCTTTGCG 
TATGTGGAAG GGGAAAGCCT GCTGATGGCA CTCAAGGACG TTGCCTTGTC CTCGGGGAGT 
GCCTGCACTT CCGCATCATT GGAGCCCTCG TATGTCCTCA GAGCCATCGG TACGGATGAG 
GATTTAGCAC ACTCCTCTAT CAGGTTCGGC ATCGGCCGCT TCACTACAGA GGAGGAGGTA 
GACTACACCG TGCAGAAGTG CATCCACCAT GTGAAACGTC TTCGAGAAAT GAGTCCTCTC 
TGGGAGATGG TGCAGGATGG CATTGACCTC AAGAGCATCA AGTGGACCCA GCATTAG