Cilk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cilk1
Entrez Gene ID	84411
Full Name	intestinal cell kinase
General protein information	Preferred Names intestinal cell kinase Names serine/threonine-protein kinase ICK MAK-related kinase MRK heart serine-threonine protein kinase intestinal cell (MAK-like) kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	may be involved in promoting meiosis during spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766345.2	XP_008764567.1	serine/threonine-protein kinase ICK isoform X1
NM_138886.1	NP_620241.1	serine/threonine-protein kinase ICK

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ICK	NP_001092357.1
Xenopus tropicalis (tropical clawed frog)	ick	XP_002934912.2
Mus musculus (house mouse)	Ick	NP_064371.2
Gallus gallus (chicken)	ICK	XP_004940534.1
Homo sapiens (human)	ICK	NP_055735.1
Rattus norvegicus (Norway rat)	Ick	NP_620241.1
	LOC711865	XP_002808434.1
Pan troglodytes (chimpanzee)	ICK	XP_001154965.2
Canis lupus familiaris (dog)	ICK	XP_005627533.1

Gene Ontology
Bibliography

Related articles in PubMed

Intestinal cell kinase, a protein associated with endocrine-cerebro-osteodysplasia syndrome, is a key regulator of cilia length and Hedgehog signaling.
Moon H, Song J, Shin JO, Lee H, Kim HK, Eggenschwiller JT, Bok J, Ko HW
Proceedings of the National Academy of Sciences of the United States of America111(23)8541-6(2014 Jun)

ICK is essential for cell type-specific ciliogenesis and the regulation of ciliary transport.
Chaya T, Omori Y, Kuwahara R, Furukawa T
The EMBO journal33(11)1227-42(2014 Jun)

Regulation of cilium length and intraflagellar transport by the RCK-kinases ICK and MOK in renal epithelial cells.
Broekhuis JR, Verhey KJ, Jansen G
PloS one9(9)e108470(2014)

A multiplex human syndrome implicates a key role for intestinal cell kinase in development of central nervous, skeletal, and endocrine systems.
Lahiry P, Wang J, Robinson JF, Turowec JP, Litchfield DW, Lanktree MB, Gloor GB, Puffenberger EG, Strauss KA, Martens MB, Ramsay DA, Rupar CA, Siu V, Hegele RA
American journal of human genetics84(2)134-47(2009 Feb)

Intestinal cell kinase (ICK) localizes to the crypt region and requires a dual phosphorylation site found in map kinases.
Togawa K, Yan YX, Inomoto T, Slaugenhaupt S, Rustgi AK
Journal of cellular physiology183(1)129-39(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cilk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cilk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01871	NM_138886.1 Latest version!	Rattus norvegicus ciliogenesis associated kinase 1 (Cilk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
ORa43831	XM_008766345.2 Latest version!	Rattus norvegicus intestinal cell (MAK-like) kinase (Ick), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cilk1 ( NM_138886.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620241.1 Rattus norvegicus ciliogenesis associated kinase 1 (Cilk1), mRNA.

Price & Availability↑

CloneID	ORa01871
Clone ID Related Accession (Same CDS sequence)	NM_138886.1
Accession Version	NM_138886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase ICK
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D26178.1. ##Evidence-Data-START## Transcript exon combination :: D26178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATAGAT ACACAACGAT CAAACAGCTT GGGGATGGGA CCTATGGTTC TGTCCTGCTG 
GGGAGAAGCA TTGAATCTGG AGAACTAATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT CAACCATGCC 
AATATAGTGA AGTTAAAAGA GGTTATCAGG GAGAACGACC ATCTCTATTT CATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT CCCAGAGTCT 
GCAATAAGGA ATATAATGTA TCAGATATTG CAAGGACTGG CGTTCATTCA CAAACATGGT 
TTCTTCCACC GGGACTTAAA GCCTGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCGGACT TCGGATTGGC CCGAGAAATC CGATCAAGAC CTCCATACAC AGACTATGTG 
TCTACTAGAT GGTATAGGGC TCCAGAAGTA CTCCTGAGAT CTACCAACTA CAGCTCCCCC 
ATTGATGTCT GGGCCGTGGG CTGCATCATG GCAGAGGTGT ACACCCTCCG GCCTCTTTTC 
CCTGGGGCCA GTGAAATTGA CACGATTTTC AAAATTTGCC AAGTGTTGGG AACACCGAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCAT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACACTAATT CCAAATGCTA GCAGCGAAGC AATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CTGCTAGTCA GGCACTTCGA 
TACCCTTACT TCCAGATCGG ACACCCACTG GGCATCAGCA CACAGGACTC GGGAAAGCCA 
CAGAAAGATG TCCAAGACAA GACAGGCCCA CCTCCTTACG TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCAA CCAAGGCGCA TACACTGATT TCTTCACGAC CAAACCAAGC CAGTCAACCC 
CATCAGCATT TTGTATATCC CTACAAGGGC GAAGCCTCTA GGACAGAGCA GCTGAGCCAC 
GTGCAGGAGG GCCAGCCAAA CCCACCATTC TTCCCGTCCC TCCACAACAA GAATCTCCCG 
CCAAAAATCC TAGCTGGCCT GGAACAGAAA AGCGGTGACA TGAAACCAAA GAGCAGGAGA 
AGATGGGGTC TCATTTCCAG GTCAACCAAG GGTTCTGATG ATTGGGCCGA CTTGGCTGAC 
TTAGATTTCA GTTCCTCTCT CACCAGAATA GATGTCAAAA ACAAGAAGAG ACAGAGTGAC 
GACCCCCTCT GCAGGTTTGA AAGTGTTTTG GACCTGAAGC CATCTGAGCC GGTGGGTACA 
GGAACTAGTG TCTCCACCCA GGCTTCCTCC CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
ACAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACCTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA GTTCATGGTC CAGTTCTGGT 
TTGTCTGGAA AATCTTCAGG AACGGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCCACGA GCTCTACTGG ACTGACTGGA AGCTACATCC CTTCCTTTCT GAAGAAGGAA 
GTCGGCTCTG TCATGCAGAG GGTCCAGCTG GCGCCTCTCG CAGCCCCTTC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCA GGGAGGCCAT TCTTTCACAC CCAGCCTAGG 
AGCACGCCTG GCTTGATTCC ACGACCTCCA GCTGTGCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC TCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620241.1
CDS	297..2186
Translation	MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFIWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGISTQDSGKPQKDVQDKTGPPPYVKPAPPAQAPTKAHTLISSRPNQASQPHQHFVYPYKGEASRTEQLSHVQEGQPNPPFFPSLHNKNLPPKILAGLEQKSGDMKPKSRRRWGLISRSTKGSDDWADLADLDFSSSLTRIDVKNKKRQSDDPLCRFESVLDLKPSEPVGTGTSVSTQASSQRRDTPTLQSTAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSSSWSSSGLSGKSSGTVSVVSKITSVGSGSTSSTGLTGSYIPSFLKKEVGSVMQRVQLAPLAAPSPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAVQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	NM_138886.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ciliogenesis associated kinase 1 (Cilk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138886.1

ORF Insert Sequence:

ATGAATAGAT ACACAACGAT CAAACAGCTT GGGGATGGGA CCTATGGTTC TGTCCTGCTG 
GGGAGAAGCA TTGAATCTGG AGAACTAATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT CAACCATGCC 
AATATAGTGA AGTTAAAAGA GGTTATCAGG GAGAACGACC ATCTCTATTT CATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT CCCAGAGTCT 
GCAATAAGGA ATATAATGTA TCAGATATTG CAAGGACTGG CGTTCATTCA CAAACATGGT 
TTCTTCCACC GGGACTTAAA GCCTGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCGGACT TCGGATTGGC CCGAGAAATC CGATCAAGAC CTCCATACAC AGACTATGTG 
TCTACTAGAT GGTATAGGGC TCCAGAAGTA CTCCTGAGAT CTACCAACTA CAGCTCCCCC 
ATTGATGTCT GGGCCGTGGG CTGCATCATG GCAGAGGTGT ACACCCTCCG GCCTCTTTTC 
CCTGGGGCCA GTGAAATTGA CACGATTTTC AAAATTTGCC AAGTGTTGGG AACACCGAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCAT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACACTAATT CCAAATGCTA GCAGCGAAGC AATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CTGCTAGTCA GGCACTTCGA 
TACCCTTACT TCCAGATCGG ACACCCACTG GGCATCAGCA CACAGGACTC GGGAAAGCCA 
CAGAAAGATG TCCAAGACAA GACAGGCCCA CCTCCTTACG TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCAA CCAAGGCGCA TACACTGATT TCTTCACGAC CAAACCAAGC CAGTCAACCC 
CATCAGCATT TTGTATATCC CTACAAGGGC GAAGCCTCTA GGACAGAGCA GCTGAGCCAC 
GTGCAGGAGG GCCAGCCAAA CCCACCATTC TTCCCGTCCC TCCACAACAA GAATCTCCCG 
CCAAAAATCC TAGCTGGCCT GGAACAGAAA AGCGGTGACA TGAAACCAAA GAGCAGGAGA 
AGATGGGGTC TCATTTCCAG GTCAACCAAG GGTTCTGATG ATTGGGCCGA CTTGGCTGAC 
TTAGATTTCA GTTCCTCTCT CACCAGAATA GATGTCAAAA ACAAGAAGAG ACAGAGTGAC 
GACCCCCTCT GCAGGTTTGA AAGTGTTTTG GACCTGAAGC CATCTGAGCC GGTGGGTACA 
GGAACTAGTG TCTCCACCCA GGCTTCCTCC CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
ACAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACCTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA GTTCATGGTC CAGTTCTGGT 
TTGTCTGGAA AATCTTCAGG AACGGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCA 
GGCTCCACGA GCTCTACTGG ACTGACTGGA AGCTACATCC CTTCCTTTCT GAAGAAGGAA 
GTCGGCTCTG TCATGCAGAG GGTCCAGCTG GCGCCTCTCG CAGCCCCTTC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCA GGGAGGCCAT TCTTTCACAC CCAGCCTAGG 
AGCACGCCTG GCTTGATTCC ACGACCTCCA GCTGTGCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC TCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cilk1 ( XM_008766345.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764567.1 Rattus norvegicus intestinal cell (MAK-like) kinase (Ick), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43831
Clone ID Related Accession (Same CDS sequence)	XM_008766345.2
Accession Version	XM_008766345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase ICK isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGAT ACACAACGAT CAAACAGCTT GGGGATGGGA CCTATGGTTC TGTCCTGCTG 
GGGAGAAGCA TTGAATCTGG AGAACTAATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT CAACCATGCC 
AATATAGTGA AGTTAAAAGA GGTTATCAGG GAGAACGACC ATCTCTATTT CATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCAATAAGGA ATATAATGTA TCAGATATTG CAAGGACTGG CGTTCATTCA CAAACATGGT 
TTCTTCCACC GGGACTTAAA GCCTGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCGGACT TCGGATTGGC CCGAGAAATC CGATCAAGAC CTCCATACAC AGACTATGTG 
TCTACTAGAT GGTATAGGGC TCCAGAAGTA CTCCTGAGAT CTACCAACTA CAGCTCCCCC 
ATTGATGTCT GGGCCGTGGG CTGCATCATG GCAGAGGTGT ACACCCTCCG GCCTCTTTTC 
CCTGGGGCCA GTGAAATTGA CACGATTTTC AAAATTTGCC AAGTGTTGGG AACACCGAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCAT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACACTAATT CCAAATGCTA GCAGCGAAGC AATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CTGCTAGTCA GGCACTTCGA 
TACCCTTACT TCCAGATCGG ACACCCACTG GGCATCAGCA CACAGGACTC GGGAAAGCCA 
CAGAAAGATG TCCAAGACAA GACAGGCCCA CCTCCTTACG TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCAA CCAAGGCGCA TACACTGATT TCTTCACGAC CAAACCAAGC CAGTCAACCC 
CATCAGCATT TTGTATATCC CTACAAGGGC GAAGCCTCTA GGACAGAGCA GCTGAGCCAC 
GTGCAGGAGG GCCAGCCAAA CCCACCATTC TTCCCGTCCC TCCACAACAA GAATCTCCCG 
CCAAAAATCC TAGCTGGCCT GGAACAGAAA AGCGGTGACA TGAAACCAAA GAGCAGGAGA 
AGATGGGGTC TCATTTCCAG GTCAACCAAG GGTTCTGATG ATTGGGCCGA CTTGGCTGAC 
TTAGATTTCA GTTCCTCTCT CACCAGAATA GATGTCAAAA ACAAGAAGAG ACAGAGTGAC 
GACCCCCTCT GCAGGTTTGA AAGTGTTTTG GACCTGAAGC CATCTGAGCC GGTGGGTACA 
GGAACTAGTG TCTCCACCCA GGCTTCCTCC CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
ACAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACCTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA GTTCATGGTC CAGTTCTGGT 
TTGTCTGGAA AATCTTCAGG AACGGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCC 
GGCTCCACGA GCTCTGGTGG ACTGACTGGA AGCTACATCC CTTCCTTTCT GAAGAAGGAA 
GTCGGCTCTG TCATGCAGAG GGTCCAGCTG GCGCCTCTCG CAGCCCCTTC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCA GGGAGGCCAT TCTTTCACAC CCAGCCTAGG 
AGCACGCCTG GCTTGATTCC ACGACCTCCA GCTGTGCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC TCGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764567.1
CDS	497..2386
Translation	MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFIWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGISTQDSGKPQKDVQDKTGPPPYVKPAPPAQAPTKAHTLISSRPNQASQPHQHFVYPYKGEASRTEQLSHVQEGQPNPPFFPSLHNKNLPPKILAGLEQKSGDMKPKSRRRWGLISRSTKGSDDWADLADLDFSSSLTRIDVKNKKRQSDDPLCRFESVLDLKPSEPVGTGTSVSTQASSQRRDTPTLQSTAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSSSWSSSGLSGKSSGTVSVVSKITSVGSGSTSSGGLTGSYIPSFLKKEVGSVMQRVQLAPLAAPSPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAVQPVHGRIDWSSKYPSRR

Target ORF information:

RefSeq Version	XM_008766345.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intestinal cell (MAK-like) kinase (Ick), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766345.2

ORF Insert Sequence:

ATGAATAGAT ACACAACGAT CAAACAGCTT GGGGATGGGA CCTATGGTTC TGTCCTGCTG 
GGGAGAAGCA TTGAATCTGG AGAACTAATT GCCATTAAAA AAATGAAAAG AAAGTTTTAC 
TCCTGGGAAG AATGCATGAA CCTTCGGGAG GTTAAGTCTT TAAAGAAGCT CAACCATGCC 
AATATAGTGA AGTTAAAAGA GGTTATCAGG GAGAACGACC ATCTCTATTT CATCTTCGAG 
TACATGAAGG AAAACCTTTA CCAGCTCATT AAAGAAAGAA ATAAGTTGTT TCCAGAGTCT 
GCAATAAGGA ATATAATGTA TCAGATATTG CAAGGACTGG CGTTCATTCA CAAACATGGT 
TTCTTCCACC GGGACTTAAA GCCTGAGAAC CTCCTCTGCA TGGGACCAGA ACTTGTGAAA 
ATCGCGGACT TCGGATTGGC CCGAGAAATC CGATCAAGAC CTCCATACAC AGACTATGTG 
TCTACTAGAT GGTATAGGGC TCCAGAAGTA CTCCTGAGAT CTACCAACTA CAGCTCCCCC 
ATTGATGTCT GGGCCGTGGG CTGCATCATG GCAGAGGTGT ACACCCTCCG GCCTCTTTTC 
CCTGGGGCCA GTGAAATTGA CACGATTTTC AAAATTTGCC AAGTGTTGGG AACACCGAAG 
AAGACTGACT GGCCTGAAGG CTACCAGCTG TCAAGTGCTA TGAATTTCAT CTGGCCCCAG 
TGCATACCCA ATAACTTAAA GACACTAATT CCAAATGCTA GCAGCGAAGC AATTCAGCTC 
CTGAGAGACT TGCTTCAGTG GGATCCCAAG AAACGACCAA CTGCTAGTCA GGCACTTCGA 
TACCCTTACT TCCAGATCGG ACACCCACTG GGCATCAGCA CACAGGACTC GGGAAAGCCA 
CAGAAAGATG TCCAAGACAA GACAGGCCCA CCTCCTTACG TCAAGCCAGC CCCTCCTGCC 
CAGGCCCCAA CCAAGGCGCA TACACTGATT TCTTCACGAC CAAACCAAGC CAGTCAACCC 
CATCAGCATT TTGTATATCC CTACAAGGGC GAAGCCTCTA GGACAGAGCA GCTGAGCCAC 
GTGCAGGAGG GCCAGCCAAA CCCACCATTC TTCCCGTCCC TCCACAACAA GAATCTCCCG 
CCAAAAATCC TAGCTGGCCT GGAACAGAAA AGCGGTGACA TGAAACCAAA GAGCAGGAGA 
AGATGGGGTC TCATTTCCAG GTCAACCAAG GGTTCTGATG ATTGGGCCGA CTTGGCTGAC 
TTAGATTTCA GTTCCTCTCT CACCAGAATA GATGTCAAAA ACAAGAAGAG ACAGAGTGAC 
GACCCCCTCT GCAGGTTTGA AAGTGTTTTG GACCTGAAGC CATCTGAGCC GGTGGGTACA 
GGAACTAGTG TCTCCACCCA GGCTTCCTCC CAGAGGAGAG ACACGCCGAC CCTGCAGTCC 
ACAGCAAAGC AGCACTACTT GAAGCACTCT CGCTACCTGC CTGGAATAAA TATAAGAAAT 
GGCGTGCTCC CAAATCCAGG CAAGGACTTT CTTCCATCAA GTTCATGGTC CAGTTCTGGT 
TTGTCTGGAA AATCTTCAGG AACGGTATCA GTAGTGAGCA AAATAACTTC AGTTGGCTCC 
GGCTCCACGA GCTCTGGTGG ACTGACTGGA AGCTACATCC CTTCCTTTCT GAAGAAGGAA 
GTCGGCTCTG TCATGCAGAG GGTCCAGCTG GCGCCTCTCG CAGCCCCTTC CCCCGGCTAC 
TCTTCCCTCA AGGCCGTGAG ACCTCACCCA GGGAGGCCAT TCTTTCACAC CCAGCCTAGG 
AGCACGCCTG GCTTGATTCC ACGACCTCCA GCTGTGCAGC CTGTGCACGG CAGGATAGAC 
TGGTCTTCCA AGTACCCATC TCGGCGGTGA