Ghsr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ghsr
Entrez Gene ID	84022
Full Name	growth hormone secretagogue receptor
General protein information	Preferred Names growth hormone secretagogue receptor Names growth hormone secretagogue receptor type 1 GH-releasing peptide receptor GHRP GHS-R ghrelin receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	has G-protein coupled receptor binding activity to ligand ghrelin; plays a role in regulation of growth hormone release [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591141.1	XP_017446630.1	growth hormone secretagogue receptor type 1 isoform X1
NM_032075.3	NP_114464.1	growth hormone secretagogue receptor type 1
XM_017591142.1	XP_017446631.1	growth hormone secretagogue receptor type 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ghsr	NP_796304.1
Pan troglodytes (chimpanzee)	GHSR	XP_003310149.1
Canis lupus familiaris (dog)	GHSR	NP_001093415.1
	GHSR	XP_001084886.1
Gallus gallus (chicken)	GHSR	NP_989725.1
Danio rerio (zebrafish)	ghsra	NP_001139744.1
Drosophila melanogaster (fruit fly)	ETHR	NP_996255.1
Xenopus tropicalis (tropical clawed frog)	ghsr	XP_002931618.1
Homo sapiens (human)	GHSR	NP_940799.1
Bos taurus (cattle)	GHSR	NP_001137208.1
Rattus norvegicus (Norway rat)	Ghsr	NP_114464.1

Gene Ontology
Bibliography

Related articles in PubMed

Ghrelin Attenuates Retinal Neuronal Autophagy and Apoptosis in an Experimental Rat Glaucoma Model.
Zhu K, Zhang ML, Liu ST, Li XY, Zhong SM, Li F, Xu GZ, Wang Z, Miao Y
Investigative ophthalmology & visual science58(14)6113-6122(2017 Dec)

Blocking constitutive activity of GHSR1a in the lateral amygdala facilitates acquisition of conditioned taste aversion.
Li N, Song G, Wang Y, Zhu Q, Han F, Zhang C, Zhou Y
Neuropeptides(2017 Dec)

Regulation of Ghrelin Receptor by Periodontal Bacteria
Nokhbehsaim M, Damanaki A, Nogueira AVB, Eick S, Memmert S, Zhou X, Nanayakkara S, G?tz W, Cirelli JA, J?ger A, Deschner J
Mediators of inflammation20174916971(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ghrelin may provide neuroprotective effect in COH retinas through activating ghrelin/GHSR-1a system, which was mediated by inhibiting retinal autophagy, ganglion cell apoptosis, and Muller cell gliosis.
Title: Ghrelin Attenuates Retinal Neuronal Autophagy and Apoptosis in an Experimental Rat Glaucoma Model.

Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets.
Title: Ghrelin doesn't limit insulin release in pregnant rats fed low protein diet.

Fasted FHH-GHSR(m1/Mcwi) rats exhibited increased Agouti-Related Peptide (AgRP) and Neuropeptide Y (NPY) expression in the Arcuate Nucleus (ARC), indicative of altered central regulation of feeding and energy balance
Title: Rats with a truncated ghrelin receptor (GHSR) do not respond to ghrelin, and show reduced intake of palatable, high-calorie food.

Data show that growth hormone secretagogue receptor Ghsr(Q343X) mutation (Ghsr(M/M)) rats had a more stable body weight under caloric restriction.
Title: Enhanced responsiveness of Ghsr Q343X rats to ghrelin results in enhanced adiposity without increased appetite.

Study demonstrated that NMDAR functions are regulated by ghrelin in a GHSR1a-dependent manner: NMDAR-mediated synaptic currents are increased as a result of GluN1 subunit phosphorylation in the hippocampal pyramidal neurons and synapses
Title: Ghrelin receptor activity amplifies hippocampal N-methyl-d-aspartate receptor-mediated postsynaptic currents and increases phosphorylation of the GluN1 subunit at Ser896 and Ser897.

The following Ghsr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ghsr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08986	NM_032075.3 Latest version!	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa24585	XM_017591141.1 Latest version!	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa24585	XM_017591142.1 Latest version!	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ghsr ( NM_032075.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114464.1 Rattus norvegicus growth hormone secretagogue receptor (Ghsr), mRNA.

Price & Availability↑

CloneID	ORa08986
Clone ID Related Accession (Same CDS sequence)	NM_032075.3
Accession Version	NM_032075.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Rattus norvegicus(Norway rat)
Product	growth hormone secretagogue receptor type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB001982.1. On Sep 3, 2003 this sequence version replaced NM_032075.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB001982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACTA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAAAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTATGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114464.1
CDS	1..1095
Translation	MWNATPSEEPEPNVTLDLDWDASPGNDSLPDELLPLFPAPLLAGVTATCVALFVVGISGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVILVIWAVAFCSAGPIFVLVGVEHENGTDPRDTNECRATEFAVRSGLLTVMVWVSSVFFFLPVFCLTVLYSLIGRKLWRRRGDAAVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFKLLGFESFSQRKLSTLKDESSRAWTKSSINT

Target ORF information:

RefSeq Version	NM_032075.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032075.3

ORF Insert Sequence:

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACTA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAAAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTATGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ghsr ( XM_017591141.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446630.1 Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24585
Clone ID Related Accession (Same CDS sequence)	XM_017591141.1 , XM_017591142.1
Accession Version	XM_017591141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	growth hormone secretagogue receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACCA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAGAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTGTGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446630.1
CDS	712..1806
Translation	MWNATPSEEPEPNVTLDLDWDASPGNDSLPDELLPLFPAPLLAGVTATCVALFVVGISGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVILVIWAVAFCSAGPIFVLVGVEHENGTDPRDTNECRATEFAVRSGLLTVMVWVSSVFFFLPVFCLTVLYSLIGRKLWRRRGDAAVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFKLLGFESFSQRKLSTLKDESSRAWTKSSINT

Target ORF information:

RefSeq Version	XM_017591141.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591141.1

ORF Insert Sequence:

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACCA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAGAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTGTGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ghsr ( XM_017591142.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446631.1 Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24585
Clone ID Related Accession (Same CDS sequence)	XM_017591141.1 , XM_017591142.1
Accession Version	XM_017591142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	growth hormone secretagogue receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACCA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAGAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTGTGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446631.1
CDS	741..1835
Translation	MWNATPSEEPEPNVTLDLDWDASPGNDSLPDELLPLFPAPLLAGVTATCVALFVVGISGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVILVIWAVAFCSAGPIFVLVGVEHENGTDPRDTNECRATEFAVRSGLLTVMVWVSSVFFFLPVFCLTVLYSLIGRKLWRRRGDAAVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFKLLGFESFSQRKLSTLKDESSRAWTKSSINT

Target ORF information:

RefSeq Version	XM_017591142.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth hormone secretagogue receptor (Ghsr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591142.1

ORF Insert Sequence:

ATGTGGAACG CGACCCCCAG CGAGGAGCCG GAGCCTAACG TCACGTTGGA CCTGGATTGG 
GACGCTTCCC CCGGCAACGA CTCACTGCCT GACGAACTGC TGCCGCTGTT CCCCGCTCCG 
CTGCTGGCAG GCGTCACCGC CACCTGCGTG GCGCTCTTCG TGGTGGGCAT CTCAGGCAAC 
CTGCTCACCA TGCTGGTGGT GTCCCGCTTC CGCGAGCTGC GCACCACCAC CAACCTCTAC 
CTGTCCAGCA TGGCCTTCTC GGATCTGCTC ATCTTCCTGT GCATGCCGCT GGACCTCGTC 
CGCCTCTGGC AGTACCGGCC CTGGAACTTC GGCGACCTGC TCTGCAAACT CTTCCAGTTT 
GTCAGCGAGA GCTGCACCTA CGCCACGGTC CTCACCATCA CCGCGCTGAG CGTCGAGCGC 
TACTTCGCCA TCTGCTTCCC TCTGCGGGCC AAGGTGGTGG TCACTAAGGG CCGCGTGAAG 
CTGGTCATCC TTGTCATCTG GGCCGTGGCT TTCTGCAGCG CGGGGCCCAT CTTCGTGCTG 
GTGGGCGTGG AGCACGAGAA CGGCACAGAT CCCCGGGACA CCAACGAATG CCGCGCCACC 
GAGTTCGCTG TGCGCTCTGG GCTGCTCACC GTCATGGTGT GGGTGTCCAG CGTCTTCTTC 
TTTCTACCGG TCTTCTGCCT CACTGTGCTC TACAGTCTCA TCGGGAGGAA GCTGTGGCGG 
AGACGCGGAG ATGCAGCGGT GGGCGCCTCG CTCCGGGACC AGAACCACAA GCAGACAGTG 
AAGATGCTTG CTGTGGTGGT GTTTGCTTTC ATCCTCTGCT GGCTGCCCTT CCACGTGGGA 
AGATACCTCT TTTCCAAGTC CTTCGAGCCT GGCTCTCTGG AGATCGCTCA GATCAGCCAG 
TACTGCAACC TGGTGTCCTT TGTCCTCTTC TACCTCAGCG CTGCCATCAA CCCCATTCTG 
TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA AACTGCTAGG ATTTGAATCC 
TTCTCCCAGA GAAAGCTTTC CACTCTGAAG GATGAGAGTT CCCGGGCCTG GACAAAGTCG 
AGCATCAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ghrelin Attenuates Retinal Neuronal Autophagy and Apoptosis in an Experimental Rat Glaucoma Model.
Investigative ophthalmology & visual science58(14)6113-6122(2017 Dec)
Zhu K,Zhang ML,Liu ST,Li XY,Zhong SM,Li F,Xu GZ,Wang Z,Miao Y

Blocking constitutive activity of GHSR1a in the lateral amygdala facilitates acquisition of conditioned taste aversion.
Neuropeptides(2017 Dec)
Li N,Song G,Wang Y,Zhu Q,Han F,Zhang C,Zhou Y

Regulation of Ghrelin Receptor by Periodontal Bacteria
Mediators of inflammation20174916971(2017)
Nokhbehsaim M,Damanaki A,Nogueira AVB,Eick S,Memmert S,Zhou X,Nanayakkara S,G?tz W,Cirelli JA,J?ger A,Deschner J

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Ghsr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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