Hsd17b12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd17b12
Entrez Gene ID	84013
Full Name	hydroxysteroid (17-beta) dehydrogenase 12
General protein information	Preferred Names hydroxysteroid (17-beta) dehydrogenase 12 Names very-long-chain 3-oxoacyl-CoA reductase 17-beta-HSD 12 17-beta-hydroxysteroid dehydrogenase 12 3-ketoacyl-CoA reductase KAR estradiol 17-beta-dehydrogenase 12 smooth muscle-specific 17 beta-hydroxysteroid dehydrogenase type 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q31
Summary	may play a role in steroid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032066.1	NP_114455.1	very-long-chain 3-oxoacyl-CoA reductase
XM_006234599.3	XP_006234661.1	very-long-chain 3-oxoacyl-CoA reductase isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	let-767	NP_001254935.1
Caenorhabditis elegans (roundworm)	stdh-4	NP_505205.2
Kluyveromyces lactis	KLLA0B09812g	XP_451965.1
Xenopus tropicalis (tropical clawed frog)	hsd17b12	NP_001017234.1
Bos taurus (cattle)	HSD17B12	NP_001094777.1
Mus musculus (house mouse)	Hsd17b12	NP_062631.1
Danio rerio (zebrafish)	hsd17b12a	NP_957175.1
Caenorhabditis elegans (roundworm)	stdh-2	NP_507092.1
Eremothecium gossypii	AGOS_ADR059C	NP_984155.2
Magnaporthe oryzae (rice blast fungus)	MGG_05096	XP_003712620.1
Neurospora crassa	NCU11297	XP_957031.1
Homo sapiens (human)	HSD17B12	NP_057226.1
Gallus gallus (chicken)	HSD17B12	XP_003641391.2
Oryza sativa (rice)	Os04g0483500	NP_001053125.1
Pan troglodytes (chimpanzee)	HSD17B12	XP_001158375.1
Canis lupus familiaris (dog)	HSD17B12	XP_848504.1
Rattus norvegicus (Norway rat)	Hsd17b12	NP_114455.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004532	XP_313833.5
Saccharomyces cerevisiae (baker's yeast)	IFA38	NP_009717.1
Schizosaccharomyces pombe (fission yeast)	SPAC4G9.15	NP_593697.1
Arabidopsis thaliana (thale cress)	KCR1	NP_564905.1
Danio rerio (zebrafish)	hsd17b12b	NP_955907.1
Drosophila melanogaster (fruit fly)	CG1444	NP_572420.1
Caenorhabditis elegans (roundworm)	stdh-3	NP_506448.2
Caenorhabditis elegans (roundworm)	stdh-1	NP_506449.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome-based systematic identification of extracellular matrix proteins.
Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)

Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade.
Moon YA, Horton JD
The Journal of biological chemistry278(9)7335-43(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsd17b12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd17b12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08952	NM_032066.1 Latest version!	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa26126	XM_006234599.3 Latest version!	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd17b12 ( NM_032066.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114455.1 Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), mRNA.

Price & Availability↑

CloneID	ORa08952
Clone ID Related Accession (Same CDS sequence)	NM_032066.1
Accession Version	NM_032066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Rattus norvegicus(Norway rat)
Product	very-long-chain 3-oxoacyl-CoA reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U81186.1. ##Evidence-Data-START## Transcript exon combination :: U81186.1, BC061543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGCG CTCTCCCGGC GGCCGGCTTC CTATACTGGG TGGGCGCGAG CACCATTGCT 
TACCTGACCC TGCGAGCCTC GTACTCGCTC TTCAGGGCCT TCCAGGTGTG GTGCGTGGGC 
AACCAGGCCT TTGTCGGCCC GCGGCTCGGA GAATGGGCAG TTGTTACAGG TGGCACTGAT 
GGAATTGGAA AATCATATGC AGAAGAGTTA GCAAAGCGTG GAATGAAGAT TGTCTTGATA 
AGCAGATCCC AGGATAAACT GAAGGAAGTT TCCAACAACA TCAAAGAAAA ATTCAACGTT 
GAAACAAGGA CCATTGCTGT CGACTTTTCA TTGGATGATA TTTATGATAA AATTAAGACA 
GGCCTGTCTG GTCTTGAAAT TGGCGTTTTA GTGAACAACG TGGGCATGTC ATACGAGTAT 
CCAGAATACT TTTTGGAAAT TCCTGACTTG GACAACACCA TCAAGAAACT GATAAATATT 
AATGTGCTTT CCATTTGCAA GGTGACACGC TTGGTGCTGC CTGGCATGGT AGAGAGATCT 
AAAGGGGTGA TTCTCAACAT CTCCTCTGCC AGTGGCATGC TCCCGGTTCC ACTGTTGACA 
GTCTACTCTG CAACCAAGGC CTTTGTAGAT TTCTTCTCTC AGTGCCTCCA TGAGGAGTAT 
AAGAGCAAGG GCATCTTTGT GCAGAGTGTC CTGCCATTCT TTGTATCTAC AAAACTGGCA 
AAAATACGGA AGCCGACTTT GGATAAGCCC TCTGCAGAGA CATTTGTGAA GTCTGCAATT 
AAAACAGTAG GTCTGCAGAC CCGAACCACT GGGATATGTG ATCCACGCCA TCATGGGCTC 
AATAAACTCA ATCTTGCCCC GTTGGATTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114455.1
CDS	10..885
Translation	MERALPAAGFLYWVGASTIAYLTLRASYSLFRAFQVWCVGNQAFVGPRLGEWAVVTGGTDGIGKSYAEELAKRGMKIVLISRSQDKLKEVSNNIKEKFNVETRTIAVDFSLDDIYDKIKTGLSGLEIGVLVNNVGMSYEYPEYFLEIPDLDNTIKKLININVLSICKVTRLVLPGMVERSKGVILNISSASGMLPVPLLTVYSATKAFVDFFSQCLHEEYKSKGIFVQSVLPFFVSTKLAKIRKPTLDKPSAETFVKSAIKTVGLQTRTTGICDPRHHGLNKLNLAPLDLF

Target ORF information:

RefSeq Version	NM_032066.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032066.1

ORF Insert Sequence:

ATGGAGCGCG CTCTCCCGGC GGCCGGCTTC CTATACTGGG TGGGCGCGAG CACCATTGCT 
TACCTGACCC TGCGAGCCTC GTACTCGCTC TTCAGGGCCT TCCAGGTGTG GTGCGTGGGC 
AACCAGGCCT TTGTCGGCCC GCGGCTCGGA GAATGGGCAG TTGTTACAGG TGGCACTGAT 
GGAATTGGAA AATCATATGC AGAAGAGTTA GCAAAGCGTG GAATGAAGAT TGTCTTGATA 
AGCAGATCCC AGGATAAACT GAAGGAAGTT TCCAACAACA TCAAAGAAAA ATTCAACGTT 
GAAACAAGGA CCATTGCTGT CGACTTTTCA TTGGATGATA TTTATGATAA AATTAAGACA 
GGCCTGTCTG GTCTTGAAAT TGGCGTTTTA GTGAACAACG TGGGCATGTC ATACGAGTAT 
CCAGAATACT TTTTGGAAAT TCCTGACTTG GACAACACCA TCAAGAAACT GATAAATATT 
AATGTGCTTT CCATTTGCAA GGTGACACGC TTGGTGCTGC CTGGCATGGT AGAGAGATCT 
AAAGGGGTGA TTCTCAACAT CTCCTCTGCC AGTGGCATGC TCCCGGTTCC ACTGTTGACA 
GTCTACTCTG CAACCAAGGC CTTTGTAGAT TTCTTCTCTC AGTGCCTCCA TGAGGAGTAT 
AAGAGCAAGG GCATCTTTGT GCAGAGTGTC CTGCCATTCT TTGTATCTAC AAAACTGGCA 
AAAATACGGA AGCCGACTTT GGATAAGCCC TCTGCAGAGA CATTTGTGAA GTCTGCAATT 
AAAACAGTAG GTCTGCAGAC CCGAACCACT GGGATATGTG ATCCACGCCA TCATGGGCTC 
AATAAACTCA ATCTTGCCCC GTTGGATTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b12 ( XM_006234599.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234661.1 Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26126
Clone ID Related Accession (Same CDS sequence)	XM_006234599.3
Accession Version	XM_006234599.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	very-long-chain 3-oxoacyl-CoA reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCG CTCTCCCGGC GGCCGGCTTC CTATACTGGG TGGGCGCGAG CACCATTGCT 
TACCTGACCC TGCGAGCCTC GTACTCGCTC TTCAGGGCCT TCCAGGTGTG GTGCGTGGGC 
AACCAGGCCT TTGTCGGCCC GCGGCTCGGA GAATGGGCAG TTGTTACAGG TGGCACTGAT 
GGAATTGGAA AATCATATGC AGAAGAGTTA GCAAAGCGTG GAATGAAGAT TGTCTTGATA 
AGCAGATCCC AGGATAAACT GAAGGAAGTT TCCAACAACA TCAAAGAAAA ATTCAACGTT 
GAAACAAGGA CCATTGCTGT CGACTTTTCA TTGGATGATA TTTATGATAA AATTAAGACA 
GGCCTGTCTG GTCTTGAAAT TGGCGTTTTA GTGAACAACG TGGGCATGTC ATACGAGTAT 
CCAGAATACT TTTTGGAAAT TCCTGACTTG GACAACACCA TCAAGAAACT GATAAATATT 
AATGTGCTTT CCATTTGCAA GGTGACACGC TTGGTGCTGC CTGGCATGGT AGAGAGATCT 
AAAGGGGTGA TTCTCAACAT CTCCTCTGCC AGTGGCATGC TCCCGGTTCC ACTGTTGACA 
GTCTACTCTG CAACCAAGGC CTTTGTAGAT TTCTTCTCTC AGTGCCTCCA TGAGGAGTAT 
AAGAGCAAGG GCATCTTTGT GCAGAGTGTC CTGCCATTCT TTGTAGCTAC AAAACTGGCA 
AAAATACGGA AGCCGACTTT GGATAAGCCC TCTGCAGAGA CATTTGTGAA GTCTGCAATT 
AAAACAGTAG GTCTGCAGAC CCGAACCACT GGATATGTGA TCCACGCCAT CATGGGCTCA 
ATAAACTCAA TCTTGCCCCG TTGGATTTAT TTTAAAACAA TCATGGGTTT CAACAAGTCT 
TTGCGGAATC GCTATCTGAA GAAAACCAAG AAGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234661.1
CDS	186..1124
Translation	MERALPAAGFLYWVGASTIAYLTLRASYSLFRAFQVWCVGNQAFVGPRLGEWAVVTGGTDGIGKSYAEELAKRGMKIVLISRSQDKLKEVSNNIKEKFNVETRTIAVDFSLDDIYDKIKTGLSGLEIGVLVNNVGMSYEYPEYFLEIPDLDNTIKKLININVLSICKVTRLVLPGMVERSKGVILNISSASGMLPVPLLTVYSATKAFVDFFSQCLHEEYKSKGIFVQSVLPFFVATKLAKIRKPTLDKPSAETFVKSAIKTVGLQTRTTGYVIHAIMGSINSILPRWIYFKTIMGFNKSLRNRYLKKTKKN

Target ORF information:

RefSeq Version	XM_006234599.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxysteroid (17-beta) dehydrogenase 12 (Hsd17b12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234599.3

ORF Insert Sequence:

ATGGAGCGCG CTCTCCCGGC GGCCGGCTTC CTATACTGGG TGGGCGCGAG CACCATTGCT 
TACCTGACCC TGCGAGCCTC GTACTCGCTC TTCAGGGCCT TCCAGGTGTG GTGCGTGGGC 
AACCAGGCCT TTGTCGGCCC GCGGCTCGGA GAATGGGCAG TTGTTACAGG TGGCACTGAT 
GGAATTGGAA AATCATATGC AGAAGAGTTA GCAAAGCGTG GAATGAAGAT TGTCTTGATA 
AGCAGATCCC AGGATAAACT GAAGGAAGTT TCCAACAACA TCAAAGAAAA ATTCAACGTT 
GAAACAAGGA CCATTGCTGT CGACTTTTCA TTGGATGATA TTTATGATAA AATTAAGACA 
GGCCTGTCTG GTCTTGAAAT TGGCGTTTTA GTGAACAACG TGGGCATGTC ATACGAGTAT 
CCAGAATACT TTTTGGAAAT TCCTGACTTG GACAACACCA TCAAGAAACT GATAAATATT 
AATGTGCTTT CCATTTGCAA GGTGACACGC TTGGTGCTGC CTGGCATGGT AGAGAGATCT 
AAAGGGGTGA TTCTCAACAT CTCCTCTGCC AGTGGCATGC TCCCGGTTCC ACTGTTGACA 
GTCTACTCTG CAACCAAGGC CTTTGTAGAT TTCTTCTCTC AGTGCCTCCA TGAGGAGTAT 
AAGAGCAAGG GCATCTTTGT GCAGAGTGTC CTGCCATTCT TTGTAGCTAC AAAACTGGCA 
AAAATACGGA AGCCGACTTT GGATAAGCCC TCTGCAGAGA CATTTGTGAA GTCTGCAATT 
AAAACAGTAG GTCTGCAGAC CCGAACCACT GGATATGTGA TCCACGCCAT CATGGGCTCA 
ATAAACTCAA TCTTGCCCCG TTGGATTTAT TTTAAAACAA TCATGGGTTT CAACAAGTCT 
TTGCGGAATC GCTATCTGAA GAAAACCAAG AAGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome-based systematic identification of extracellular matrix proteins.
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)
Manabe R,Tsutsui K,Yamada T,Kimura M,Nakano I,Shimono C,Sanzen N,Furutani Y,Fukuda T,Oguri Y,Shimamoto K,Kiyozumi D,Sato Y,Sado Y,Senoo H,Yamashina S,Fukuda S,Kawai J,Sugiura N,Kimata K,Hayashizaki Y,Sekiguchi K

Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade.
The Journal of biological chemistry278(9)7335-43(2003 Feb)
Moon YA,Horton JD