Ctcf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctcf
Entrez Gene ID	83726
Full Name	CCCTC-binding factor
General protein information	Preferred Names CCCTC-binding factor Names transcriptional repressor CTCF 11-zinc finger protein CCCTC-binding factor (zinc finger protein) CTCFL paralog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	binds to and protects the H19 differentially methylated domain from de novo methylation during oocyte growth and is involved in normal preimplantation development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255536.2	XP_006255598.1	transcriptional repressor CTCF isoform X1
NM_031824.1	NP_114012.1	transcriptional repressor CTCF

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTCF	NP_001069216.1
	CTCF	XP_001091862.2
Mus musculus (house mouse)	Ctcf	NP_851839.1
Gallus gallus (chicken)	CTCF	NP_990663.1
Danio rerio (zebrafish)	ctcf	NP_001001844.1
Xenopus tropicalis (tropical clawed frog)	ctcf	NP_001116268.1
Homo sapiens (human)	CTCF	NP_006556.1
Pan troglodytes (chimpanzee)	CTCF	XP_511035.2
Canis lupus familiaris (dog)	CTCF	XP_864432.1
Rattus norvegicus (Norway rat)	Ctcf	NP_114012.1

Gene Ontology
Bibliography

Related articles in PubMed

CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic.
Marshall AD, Bailey CG, Champ K, Vellozzi M, O'Young P, Metierre C, Feng Y, Thoeng A, Richards AM, Schmitz U, Biro M, Jayasinghe R, Ding L, Anderson L, Mardis ER, Rasko JEJ
Oncogene36(29)4100-4110(2017 Jul)

Identification of miR-34 regulatory networks in settings of disease and antimiR-therapy: Implications for treating cardiac pathology and other diseases.
Ooi JYY, Bernardo BC, Singla S, Patterson NL, Lin RCY, McMullen JR
RNA biology14(5)500-513(2017 May)

CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites.
Xiao T, Wongtrakoongate P, Trainor C, Felsenfeld G
Cell reports12(10)1704-14(2015 Sep)

Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors.
Wajapeyee N, Malonia SK, Palakurthy RK, Green MR
Genes & development27(20)2221-6(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ctcf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctcf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08821	NM_031824.1 Latest version!	Rattus norvegicus CCCTC-binding factor (Ctcf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa38888	XM_006255536.2 Latest version!	Rattus norvegicus CCCTC-binding factor (Ctcf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctcf ( NM_031824.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114012.1 Rattus norvegicus CCCTC-binding factor (Ctcf), mRNA.

Price & Availability↑

CloneID	ORa08821
Clone ID Related Accession (Same CDS sequence)	NM_031824.1
Accession Version	NM_031824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor CTCF
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF133731.2. Sequence Note: This sequence has been modified as follows: removed 36 bp suspected to be vector contamination from the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF133731.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGTG AGGCGGTTGA AGCCATTGTG GAAGAGTCTG AAACTTTCAT TAAAGGAAAG 
GAAAGAAAGA CTTACCAGAG ACGCCGGGAA GGGGGCCAGG AAGAAGATGC TTGCCACCTA 
CCCCAGAACC AGACAGACGG GGGTGAGGTG GTCCAGGATG TCAACAGTAG CGTACAGATG 
GTAATGATGG AACAGCTGGA CCCTACCCTT CTCCAAATGA AGACTGAAGT AATGGAGGGG 
ACAGTGGCTC CTGAAGCAGA AGCTGCAGTG GACGATACCC AAATCATAAC CCTGCAGGTT 
GTAAACATGG AGGAACAGCC CATTAATATA GGAGAACTTC AGCTTGTCCA AGTACCTGTT 
CCTGTGACTG TACCTGTTGC TACTACGTCA GTAGAAGAAC TTCAGGGGGC TTATGAGAAT 
GAAGTGTCTA AAGAGGGCCT TGCAGAAAGT GAACCGATGA TATGTCACAC CTTACCTTTA 
CCTGAAGGAT TTCAGGTGGT AAAAGTGGGG GCCAATGGAG AAGTGGAGAC ACTAGAGCAG 
GGGGAGCTTC CTCCTCAGGA AGACCCTAGC TGGCAAAAAG ACCCAGACTA TCAGCCACCA 
GCCAAAAAAA CAAAGAAAAC CAAAAAGAGT AAACTTCGTT ACACAGAGGA GGGCAAAGAC 
GTGGATGTGT CTGTGTATGA TTTTGAGGAA GAACAGCAGG AAGGACTGCT GTCTGAGGTT 
AATGCAGAGA AAGTGGTTGG TAATATGAAG CCTCCGAAGC CAACAAAAAT TAAAAAAAAA 
GGTGTAAAGA AAACATTCCA GTGTGAGCTT TGCAGTTACA CGTGTCCACG GCGTTCAAAT 
TTGGATCGTC ACATGAAAAG CCACACTGAT GAGAGACCAC ACAAGTGCCA TCTCTGTGGC 
AGAGCATTCA GAACAGTAAC CCTCCTGAGG AATCATCTTA ACACACACAC AGGTACTCGT 
CCTCACAAGT GCCCCGACTG CGACATGGCT TTTGTGACCA GTGGAGAGTT GGTGCGGCAT 
CGTCGTTATA AACACACTCA TGAGAAACCA TTTAAGTGTT CCATGTGTGA TTATGCCAGT 
GTAGAAGTCA GCAAATTAAA ACGACACATT CGCTCTCATA CTGGAGAGCG CCCGTTCCAG 
TGCAGTTTGT GCAGTTATGC CAGCAGGGAC ACATACAAGC TGAAAAGGCA TATGAGAACC 
CATTCAGGTG AAAAACCTTA TGAATGTTAT ATTTGTCATG CTCGGTTTAC CCAGAGTGGT 
ACCATGAAGA TGCACATTTT ACAGAAGCAC ACAGAAAATG TGGCCAAATT TCATTGTCCC 
CACTGTGACA CTGTCATAGC CCGAAAAAGT GATTTGGGTG TCCACTTGAG AAAGCAGCAT 
TCCTATATTG AGCAGGGCAA AAAATGTCGC TACTGTGATG CTGTGTTTCA TGAGAGATAT 
GCTCTTATCC AGCATCAAAA ATCACACAAA AATGAGAAGC GCTTCAAGTG CGACCAGTGT 
GATTATGCTT GTAGACAGGA GAGGCACATG ATCATGCACA AGCGCACTCA CACGGGGGAG 
AAGCCTTATG CCTGCAGCCA CTGCGACAAG ACCTTCCGCC AGAAACAGCT CCTTGACATG 
CATTTCAAGC GCTATCATGA TCCCAACTTT GTCCCCGCTG CCTTTGTCTG TTCTAAGTGT 
GGGAAAACAT TCACACGCCG GAACACAATG GCGAGACACG CAGATAACTG TGCTGGTCCA 
GACGGTGTAG AAGGGGAAAA TGGAGGAGAG ACAAAGAAGA GCAAACGAGG AAGAAAAAGA 
AAGATGCGGT CTAAAAAAGA AGACTCCTCT GACAGTGAAA ATGCTGAGCC GGATCTCGAT 
GACAATGAGG AGGAGGAGGA GCCTGCTGTA GAAATTGAAC CTGAGCCAGA GCCGCAGCCG 
CAGCCTCAGC CACAGCCGCA GCCGCAGCCT GTGGCCCCAG CCCCACCACC TGCCAAGAAG 
AGAAGAGGGC GACCTCCTGG AAGAACCAAC CAACCCAAAC AGAACCAGCC AACAGCCATC 
ATCCAGGTTG AAGACCAGAA TACAGGTGCA ATTGAGAACA TTATAGTTGA AGTCAAAAAA 
GAGCCAGATG CCGAGCCTGC GGAGGGGGAA GAGGAGGAGG CTCAGGCAGC ACCCGCAGAC 
GCCCCCAACG GAGACCTCAC GCCTGAGATG ATTCTCAGCA TGATGGACCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114012.1
CDS	169..2382
Translation	MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEEEEEPAVEIEPEPEPQPQPQPQPQPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQAAPADAPNGDLTPEMILSMMDR

Target ORF information:

RefSeq Version	NM_031824.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CCCTC-binding factor (Ctcf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031824.1

ORF Insert Sequence:

ATGGAAGGTG AGGCGGTTGA AGCCATTGTG GAAGAGTCTG AAACTTTCAT TAAAGGAAAG 
GAAAGAAAGA CTTACCAGAG ACGCCGGGAA GGGGGCCAGG AAGAAGATGC TTGCCACCTA 
CCCCAGAACC AGACAGACGG GGGTGAGGTG GTCCAGGATG TCAACAGTAG CGTACAGATG 
GTAATGATGG AACAGCTGGA CCCTACCCTT CTCCAAATGA AGACTGAAGT AATGGAGGGG 
ACAGTGGCTC CTGAAGCAGA AGCTGCAGTG GACGATACCC AAATCATAAC CCTGCAGGTT 
GTAAACATGG AGGAACAGCC CATTAATATA GGAGAACTTC AGCTTGTCCA AGTACCTGTT 
CCTGTGACTG TACCTGTTGC TACTACGTCA GTAGAAGAAC TTCAGGGGGC TTATGAGAAT 
GAAGTGTCTA AAGAGGGCCT TGCAGAAAGT GAACCGATGA TATGTCACAC CTTACCTTTA 
CCTGAAGGAT TTCAGGTGGT AAAAGTGGGG GCCAATGGAG AAGTGGAGAC ACTAGAGCAG 
GGGGAGCTTC CTCCTCAGGA AGACCCTAGC TGGCAAAAAG ACCCAGACTA TCAGCCACCA 
GCCAAAAAAA CAAAGAAAAC CAAAAAGAGT AAACTTCGTT ACACAGAGGA GGGCAAAGAC 
GTGGATGTGT CTGTGTATGA TTTTGAGGAA GAACAGCAGG AAGGACTGCT GTCTGAGGTT 
AATGCAGAGA AAGTGGTTGG TAATATGAAG CCTCCGAAGC CAACAAAAAT TAAAAAAAAA 
GGTGTAAAGA AAACATTCCA GTGTGAGCTT TGCAGTTACA CGTGTCCACG GCGTTCAAAT 
TTGGATCGTC ACATGAAAAG CCACACTGAT GAGAGACCAC ACAAGTGCCA TCTCTGTGGC 
AGAGCATTCA GAACAGTAAC CCTCCTGAGG AATCATCTTA ACACACACAC AGGTACTCGT 
CCTCACAAGT GCCCCGACTG CGACATGGCT TTTGTGACCA GTGGAGAGTT GGTGCGGCAT 
CGTCGTTATA AACACACTCA TGAGAAACCA TTTAAGTGTT CCATGTGTGA TTATGCCAGT 
GTAGAAGTCA GCAAATTAAA ACGACACATT CGCTCTCATA CTGGAGAGCG CCCGTTCCAG 
TGCAGTTTGT GCAGTTATGC CAGCAGGGAC ACATACAAGC TGAAAAGGCA TATGAGAACC 
CATTCAGGTG AAAAACCTTA TGAATGTTAT ATTTGTCATG CTCGGTTTAC CCAGAGTGGT 
ACCATGAAGA TGCACATTTT ACAGAAGCAC ACAGAAAATG TGGCCAAATT TCATTGTCCC 
CACTGTGACA CTGTCATAGC CCGAAAAAGT GATTTGGGTG TCCACTTGAG AAAGCAGCAT 
TCCTATATTG AGCAGGGCAA AAAATGTCGC TACTGTGATG CTGTGTTTCA TGAGAGATAT 
GCTCTTATCC AGCATCAAAA ATCACACAAA AATGAGAAGC GCTTCAAGTG CGACCAGTGT 
GATTATGCTT GTAGACAGGA GAGGCACATG ATCATGCACA AGCGCACTCA CACGGGGGAG 
AAGCCTTATG CCTGCAGCCA CTGCGACAAG ACCTTCCGCC AGAAACAGCT CCTTGACATG 
CATTTCAAGC GCTATCATGA TCCCAACTTT GTCCCCGCTG CCTTTGTCTG TTCTAAGTGT 
GGGAAAACAT TCACACGCCG GAACACAATG GCGAGACACG CAGATAACTG TGCTGGTCCA 
GACGGTGTAG AAGGGGAAAA TGGAGGAGAG ACAAAGAAGA GCAAACGAGG AAGAAAAAGA 
AAGATGCGGT CTAAAAAAGA AGACTCCTCT GACAGTGAAA ATGCTGAGCC GGATCTCGAT 
GACAATGAGG AGGAGGAGGA GCCTGCTGTA GAAATTGAAC CTGAGCCAGA GCCGCAGCCG 
CAGCCTCAGC CACAGCCGCA GCCGCAGCCT GTGGCCCCAG CCCCACCACC TGCCAAGAAG 
AGAAGAGGGC GACCTCCTGG AAGAACCAAC CAACCCAAAC AGAACCAGCC AACAGCCATC 
ATCCAGGTTG AAGACCAGAA TACAGGTGCA ATTGAGAACA TTATAGTTGA AGTCAAAAAA 
GAGCCAGATG CCGAGCCTGC GGAGGGGGAA GAGGAGGAGG CTCAGGCAGC ACCCGCAGAC 
GCCCCCAACG GAGACCTCAC GCCTGAGATG ATTCTCAGCA TGATGGACCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctcf ( XM_006255536.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255598.1 Rattus norvegicus CCCTC-binding factor (Ctcf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38888
Clone ID Related Accession (Same CDS sequence)	XM_006255536.2
Accession Version	XM_006255536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor CTCF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTG AGGCGGTTGA AGCCATTGTG GAAGAGTCTG AAACTTTCAT TAAAGGAAAG 
GAAAGAAAGA CTTACCAGAG ACGCCGGGAA GGGGGCCAGG AAGAAGATGC TTGCCACCTA 
CCCCAGAACC AGACAGACGG GGGTGAGGTG GTCCAGGATG TCAACAGTAG CGTACAGATG 
GTAATGATGG AACAGCTGGA CCCTACCCTT CTCCAAATGA AGACTGAAGT AATGGAGGGG 
ACAGTGGCTC CTGAAGCAGA AGCTGCAGTG GACGATACCC AAATCATAAC CCTGCAGGTT 
GTAAACATGG AGGAACAGCC CATTAATATA GGAGAACTTC AGCTTGTCCA AGTACCTGTT 
CCTGTGACTG TACCTGTTGC TACTACGTCA GTAGAAGAAC TTCAGGGGGC TTATGAGAAT 
GAAGTGTCTA AAGAGGGCCT TGCAGAAAGT GAACCGATGA TATGTCACAC CTTACCTTTA 
CCTGAAGGAT TTCAGGTGGT AAAAGTGGGG GCCAATGGAG AAGTGGAGAC ACTAGAGCAG 
GGGGAGCTTC CTCCTCAGGA AGACCCTAGC TGGCAAAAAG ACCCAGACTA TCAGCCACCA 
GCCAAAAAAA CAAAGAAAAC CAAAAAGAGT AAACTTCGTT ACACAGAGGA GGGCAAAGAC 
GTGGATGTGT CTGTGTATGA TTTTGAGGAA GAACAGCAGG AAGGTCTGCT GTCTGAGGTT 
AATGCAGAGA AAGTGGTTGG TAATATGAAG CCTCCGAAGC CAACAAAAAT TAAAAAAAAA 
GGTGTAAAGA AAACATTCCA GTGTGAGCTT TGCAGTTACA CGTGTCCACG GCGTTCAAAT 
TTGGATCGTC ACATGAAAAG CCACACTGAT GAGAGACCAC ACAAGTGCCA TCTCTGTGGC 
AGAGCATTCA GAACAGTAAC CCTCCTGAGG AATCATCTTA ACACACACAC AGGTACTCGT 
CCTCACAAGT GCCCCGACTG CGACATGGCT TTTGTGACCA GTGGAGAGTT GGTGCGGCAT 
CGTCGTTATA AACACACTCA TGAGAAACCA TTTAAGTGTT CCATGTGTGA TTATGCCAGT 
GTAGAAGTCA GCAAATTAAA ACGACACATT CGCTCTCATA CTGGAGAGCG CCCGTTCCAG 
TGCAGTTTGT GCAGTTATGC CAGCAGGGAC ACATACAAGC TGAAAAGGCA TATGAGAACC 
CATTCAGGTG AAAAACCTTA TGAATGTTAT ATTTGTCATG CTCGGTTTAC CCAGAGTGGT 
ACCATGAAGA TGCACATTTT ACAGAAGCAC ACAGAAAATG TGGCCAAATT TCATTGTCCC 
CACTGTGACA CTGTCATAGC CCGAAAAAGT GATTTGGGTG TCCACTTGAG AAAGCAGCAT 
TCCTATATTG AGCAGGGCAA AAAATGTCGC TACTGTGATG CTGTGTTTCA TGAGAGATAT 
GCTCTTATCC AGCATCAAAA ATCACACAAA AATGAGAAGC GCTTCAAGTG CGACCAGTGT 
GATTATGCTT GTAGACAGGA GAGGCACATG ATCATGCACA AGCGCACTCA CACGGGGGAG 
AAGCCTTATG CCTGCAGCCA CTGCGACAAG ACCTTCCGCC AGAAACAGCT CCTTGACATG 
CATTTCAAGC GCTATCATGA TCCCAACTTT GTCCCCGCTG CCTTTGTCTG TTCTAAGTGT 
GGGAAAACAT TCACACGCCG GAACACAATG GCGAGACACG CAGATAACTG TGCTGGTCCA 
GACGGTGTAG AAGGGGAAAA TGGAGGAGAG ACAAAGAAGA GCAAACGAGG AAGAAAAAGA 
AAGATGCGGT CTAAAAAAGA AGACTCCTCT GACAGTGAAG AAAATGCTGA GCCGGATCTC 
GATGACAATG AGGAGGAGGA GGAGCCTGCT GTAGAAATTG AACCTGAGCC AGAGCCGCAG 
CCGCAGCCTC AGCCACAGCC GCAGCCGCAG CCTGTGGCCC CAGCCCCACC ACCTGCCAAG 
AAGAGAAGAG GGCGACCTCC TGGAAGAACC AACCAACCCA AACAGAACCA GCCAACAGCC 
ATCATCCAGG TTGAAGACCA GAATACAGGT GCAATTGAGA ACATTATAGT TGAAGTCAAA 
AAAGAGCCAG ATGCCGAGCC TGCGGAGGGG GAAGAGGAGG AGGCTCAGGC AGCACCCGCA 
GACGCCCCCA ACGGAGACCT CACGCCTGAG ATGATTCTCA GCATGATGGA CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255598.1
CDS	395..2611
Translation	MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSEENAEPDLDDNEEEEEPAVEIEPEPEPQPQPQPQPQPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQAAPADAPNGDLTPEMILSMMDR

Target ORF information:

RefSeq Version	XM_006255536.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CCCTC-binding factor (Ctcf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255536.2

ORF Insert Sequence:

ATGGAAGGTG AGGCGGTTGA AGCCATTGTG GAAGAGTCTG AAACTTTCAT TAAAGGAAAG 
GAAAGAAAGA CTTACCAGAG ACGCCGGGAA GGGGGCCAGG AAGAAGATGC TTGCCACCTA 
CCCCAGAACC AGACAGACGG GGGTGAGGTG GTCCAGGATG TCAACAGTAG CGTACAGATG 
GTAATGATGG AACAGCTGGA CCCTACCCTT CTCCAAATGA AGACTGAAGT AATGGAGGGG 
ACAGTGGCTC CTGAAGCAGA AGCTGCAGTG GACGATACCC AAATCATAAC CCTGCAGGTT 
GTAAACATGG AGGAACAGCC CATTAATATA GGAGAACTTC AGCTTGTCCA AGTACCTGTT 
CCTGTGACTG TACCTGTTGC TACTACGTCA GTAGAAGAAC TTCAGGGGGC TTATGAGAAT 
GAAGTGTCTA AAGAGGGCCT TGCAGAAAGT GAACCGATGA TATGTCACAC CTTACCTTTA 
CCTGAAGGAT TTCAGGTGGT AAAAGTGGGG GCCAATGGAG AAGTGGAGAC ACTAGAGCAG 
GGGGAGCTTC CTCCTCAGGA AGACCCTAGC TGGCAAAAAG ACCCAGACTA TCAGCCACCA 
GCCAAAAAAA CAAAGAAAAC CAAAAAGAGT AAACTTCGTT ACACAGAGGA GGGCAAAGAC 
GTGGATGTGT CTGTGTATGA TTTTGAGGAA GAACAGCAGG AAGGTCTGCT GTCTGAGGTT 
AATGCAGAGA AAGTGGTTGG TAATATGAAG CCTCCGAAGC CAACAAAAAT TAAAAAAAAA 
GGTGTAAAGA AAACATTCCA GTGTGAGCTT TGCAGTTACA CGTGTCCACG GCGTTCAAAT 
TTGGATCGTC ACATGAAAAG CCACACTGAT GAGAGACCAC ACAAGTGCCA TCTCTGTGGC 
AGAGCATTCA GAACAGTAAC CCTCCTGAGG AATCATCTTA ACACACACAC AGGTACTCGT 
CCTCACAAGT GCCCCGACTG CGACATGGCT TTTGTGACCA GTGGAGAGTT GGTGCGGCAT 
CGTCGTTATA AACACACTCA TGAGAAACCA TTTAAGTGTT CCATGTGTGA TTATGCCAGT 
GTAGAAGTCA GCAAATTAAA ACGACACATT CGCTCTCATA CTGGAGAGCG CCCGTTCCAG 
TGCAGTTTGT GCAGTTATGC CAGCAGGGAC ACATACAAGC TGAAAAGGCA TATGAGAACC 
CATTCAGGTG AAAAACCTTA TGAATGTTAT ATTTGTCATG CTCGGTTTAC CCAGAGTGGT 
ACCATGAAGA TGCACATTTT ACAGAAGCAC ACAGAAAATG TGGCCAAATT TCATTGTCCC 
CACTGTGACA CTGTCATAGC CCGAAAAAGT GATTTGGGTG TCCACTTGAG AAAGCAGCAT 
TCCTATATTG AGCAGGGCAA AAAATGTCGC TACTGTGATG CTGTGTTTCA TGAGAGATAT 
GCTCTTATCC AGCATCAAAA ATCACACAAA AATGAGAAGC GCTTCAAGTG CGACCAGTGT 
GATTATGCTT GTAGACAGGA GAGGCACATG ATCATGCACA AGCGCACTCA CACGGGGGAG 
AAGCCTTATG CCTGCAGCCA CTGCGACAAG ACCTTCCGCC AGAAACAGCT CCTTGACATG 
CATTTCAAGC GCTATCATGA TCCCAACTTT GTCCCCGCTG CCTTTGTCTG TTCTAAGTGT 
GGGAAAACAT TCACACGCCG GAACACAATG GCGAGACACG CAGATAACTG TGCTGGTCCA 
GACGGTGTAG AAGGGGAAAA TGGAGGAGAG ACAAAGAAGA GCAAACGAGG AAGAAAAAGA 
AAGATGCGGT CTAAAAAAGA AGACTCCTCT GACAGTGAAG AAAATGCTGA GCCGGATCTC 
GATGACAATG AGGAGGAGGA GGAGCCTGCT GTAGAAATTG AACCTGAGCC AGAGCCGCAG 
CCGCAGCCTC AGCCACAGCC GCAGCCGCAG CCTGTGGCCC CAGCCCCACC ACCTGCCAAG 
AAGAGAAGAG GGCGACCTCC TGGAAGAACC AACCAACCCA AACAGAACCA GCCAACAGCC 
ATCATCCAGG TTGAAGACCA GAATACAGGT GCAATTGAGA ACATTATAGT TGAAGTCAAA 
AAAGAGCCAG ATGCCGAGCC TGCGGAGGGG GAAGAGGAGG AGGCTCAGGC AGCACCCGCA 
GACGCCCCCA ACGGAGACCT CACGCCTGAG ATGATTCTCA GCATGATGGA CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic.
Oncogene36(29)4100-4110(2017 Jul)
Marshall AD,Bailey CG,Champ K,Vellozzi M,O'Young P,Metierre C,Feng Y,Thoeng A,Richards AM,Schmitz U,Biro M,Jayasinghe R,Ding L,Anderson L,Mardis ER,Rasko JEJ

Identification of miR-34 regulatory networks in settings of disease and antimiR-therapy: Implications for treating cardiac pathology and other diseases.
RNA biology14(5)500-513(2017 May)
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CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites.
Cell reports12(10)1704-14(2015 Sep)
Xiao T,Wongtrakoongate P,Trainor C,Felsenfeld G

Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors.
Genes & development27(20)2221-6(2013 Oct)
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