Git1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Git1
Entrez Gene ID	83709
Full Name	GIT ArfGAP 1
General protein information	Preferred Names GIT ArfGAP 1 Names ARF GTPase-activating protein GIT1 ARF GAP GIT1 CAT-1 CAT1 G protein-coupled receptor kinase interacting ArfGAP 1 G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein (GIT1) G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 cool-associated and tyrosine-phosphorylated protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	mediates beta2-adrenergic receptor endocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031814.1	NP_114002.1	ARF GTPase-activating protein GIT1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC568935	XP_697386.4
Mus musculus (house mouse)	Git1	NP_001004144.1
Gallus gallus (chicken)	GIT1	NP_989627.1
Homo sapiens (human)	GIT1	NP_001078923.1
	GIT1	XP_001108023.2
Rattus norvegicus (Norway rat)	Git1	NP_114002.1
Pan troglodytes (chimpanzee)	GIT1	XP_511377.3
Canis lupus familiaris (dog)	GIT1	XP_005625057.1
Bos taurus (cattle)	GIT1	XP_002695750.3

Gene Ontology
Bibliography

Related articles in PubMed

?-PIX plays important role in regulation of intestinal epithelial restitution by interacting with GIT1 and Rac1 after wounding.
Rathor N, Chung HK, Wang SR, Qian M, Turner DJ, Wang JY, Rao JN
American journal of physiology. Gastrointestinal and liver physiologyajpgi002962017(2017 Nov)

Presynaptic Deletion of GIT Proteins Results in Increased Synaptic Strength at a Mammalian Central Synapse.
Montesinos MS, Dong W, Goff K, Das B, Guerrero-Given D, Schmalzigaug R, Premont RT, Satterfield R, Kamasawa N, Young SM Jr
Neuron88(5)918-925(2015 Dec)

Integrin-?1 regulates chondrocyte proliferation and apoptosis through the upregulation of GIT1 expression.
Zhang LQ, Zhao GZ, Xu XY, Fang J, Chen JM, Li JW, Gao XJ, Hao LJ, Chen YZ
International journal of molecular medicine35(4)1074-80(2015 Apr)

PDGF regulates chondrocyte proliferation through activation of the GIT1- and PLC?1-mediated ERK1/2 signaling pathway.
Xiao J, Chen X, Xu L, Zhang Y, Yin Q, Wang F
Molecular medicine reports10(5)2409-14(2014 Nov)

GIT1 and ?PIX are essential for GABA(A) receptor synaptic stability and inhibitory neurotransmission.
Smith KR, Davenport EC, Wei J, Li X, Pathania M, Vaccaro V, Yan Z, Kittler JT
Cell reports9(1)298-310(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Integrin-beta1 regulates chondrocyte proliferation and apoptosis through the upregulation of GIT1 expression
Title: Integrin-?1 regulates chondrocyte proliferation and apoptosis through the upregulation of GIT1 expression.

PDGF activates the GIT1PLCG1mediated ERK1/2 pathway to control chondrocyte proliferation.
Title: PDGF regulates chondrocyte proliferation through activation of the GIT1- and PLC?1-mediated ERK1/2 signaling pathway.

Plateletderived growth factor inhibits osteoblast apoptosis through upregulating GIT1 expression.
Title: Platelet?derived growth factor promotes osteoblast proliferation by activating G?protein?coupled receptor kinase interactor?1.

GIT1-eNOS complex is formed and regulated. GIT1 was phosphorylated on tyrosine by Src.
Title: Endothelial nitric-oxide synthase (eNOS) is activated through G-protein-coupled receptor kinase-interacting protein 1 (GIT1) tyrosine phosphorylation and Src protein.

binding of GluN3A to GIT1 is regulated by synaptic activity, a response that might restrict the negative regulatory effects of GluN3A on actin signaling to inactive synapses.
Title: GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling.

The following Git1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Git1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12027	NM_031814.1 Latest version!	Rattus norvegicus GIT ArfGAP 1 (Git1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Git1 ( NM_031814.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114002.1 Rattus norvegicus GIT ArfGAP 1 (Git1), mRNA.

Price & Availability↑

CloneID	ORa12027
Clone ID Related Accession (Same CDS sequence)	NM_031814.1
Accession Version	NM_031814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	ARF GTPase-activating protein GIT1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF085693.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF085693.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC TGACCCTGGC 
TGGGCATCTA TCAGCAGAGG TGTGCTGGTT TGTGACGAGT GCTGCAGTGT GCACCGGAGC 
CTGGGACGAC ACATCTCCAT TGTCAAACAC CTTCGCCACA GCGCCTGGCC TCCTACGCTG 
CTACAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CTATCTGGGA GCATTCCCTG 
CTGGACCCTG CACAAGTGCA AAGTGGCCGG CGCAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CAGAGTTCAT CAGGGCAAAG TACCAGATGC TGGCGTTTGT GCACAAGCTT 
CCCTGCCGTG ATGATGATGG GGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCGAGT 
GTGCGAACAG GCAACCTGGA GACCTGTCTG CGCCTGCTTT CCCTGGGTGC CCAGGCCAAC 
TTCTTCCACC CAGAAAAGGG CACTACACCT CTACACGTGG CTGCCAAGGC AGGGCAGACA 
CTGCAGGCTG AGCTGCTAGT AGTGTATGGG GCTGACCCAG GCTCCCCTGA TGTCAATGGC 
CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AACTGGCAGA AAGGCTAGTT 
GAGTGCCAGT ATGAGCTCAC TGACCGGTTG GCCTTCTACC TCTGTGGACG GAAGCCTGAT 
CACAAGAATG GGCATTACAT CATACCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG 
TCTCAGAGTT TGGATCTGTC TGAATTGGCC AAAGCTGCCA AGAAGAAGCT ACAAGCACTC 
AGCAACCGGC TCTTTGAGGA GCTCGCCATG GATGTGTATG ACGAAGTGGA CCGGAGAGAA 
AATGATGCAG TGTGGCTAGC TACCCAGAAT CATAGCACCC TGGTGACAGA ACGCAGTGCT 
GTGCCCTTCC TGCCAGTCAA CCCTGAATAC TCAGCCACTC GGAATCAGGG ACGACAGAAA 
TTGGCTCGTT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATCCT CAGTGAGGCC 
AAACGGAGGC AGCAGGGCAA GAGTCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTGCA 
CGGAACCAAA GTGACCTGGA CGACCAGCAC GACTACGATA GTGTGGCTTC TGACGAAGAC 
ACAGACCAGG AGCCCTTGCC CAGTGCTGGC GCCACGCGGA ACAATCGTGC CAGGAGCATG 
GACTCCTCAG ATCTGTCGGA TGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG 
GCTCTGGCTA CCTCCGAGGC CAAAGTGCAG CAGCTCATGA AAGTCAACAG CAGTCTGAGT 
GACGAGCTTC GGAAGCTGCA GAGGGAGATC CACAAACTGC AGGCAGAGAA CCTGCAGCTC 
CGGCAGCCAC CAGGACCAGT GCCTGTACCC TCACTTCCCA GTGAACGGGC AGAACACACA 
CTCATGGGCC CTGGTGGAAG TACCCATCGC AGGGACCGCC AGGCCTTCTC TATGTATGAG 
CCAGGCTCCG CCCTGAAGCC CTTTGGAGGT GCACCTGGGG ATGAGCTCGC CACACGGCTC 
CAGCCTTTCC ACAGCACAGA GCTGGAAGAT GATGCCATCT ATTCAGTACA TGTCCCTGCT 
GGCCTTTACC GGATCCGGAA GGGGGTGTCT GCCTCTTCTG TGACCTTCAC TCCCTCCTCC 
CCACTGCTGT CAAGCTCCCA AGAAGGAAGT CGCCATGCGA GCAAGCTTTC ACGCCACGGC 
AGTGGCGCAG AGAGTGACTA TGAGAACACA CAGAGTGGAG AGCCTCTGCT GGGACTTGAA 
GGGAAGCGAT TCCTAGAGCT GAGCAAGGAG GATGAGCTGC ACGCTGAGCT GGAGAGCTTA 
GATGGAGACC CAGACCCCGG GCTCCCCAGC ACAGAAGATG TCATCCTAAA GACAGAGCAG 
GTCACCAAGA ACATTCAGGA GCTATTGCGG GCAGCCCAGG AGTTCAAACA TGACAGCTTT 
GTGCCCTGTT CAGAAAAGAT CCATTTGGCT GTGACTGAGA TGGCCTCTCT CTTCCCAAAG 
AGGCCAGCCC TGGAGCCTGT GCGCAGTTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG 
CTGCAGAGTG AATGCCGGAA GACAGTGCCT CCAGAACCTG GCGCCCCTGT GGACTTCCAG 
CTACTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG 
GTCACCATCA CCACCCGAGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114002.1
CDS	53..2365
Translation	MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSARNQSDLDDQHDYDSVASDEDTDQEPLPSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRKLQREIHKLQAENLQLRQPPGPVPVPSLPSERAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGAPGDELATRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASSVTFTPSSPLLSSSQEGSRHASKLSRHGSGAESDYENTQSGEPLLGLEGKRFLELSKEDELHAELESLDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	NM_031814.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GIT ArfGAP 1 (Git1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031814.1

ORF Insert Sequence:

ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCGGACT GCAGCGCCCC TGACCCTGGC 
TGGGCATCTA TCAGCAGAGG TGTGCTGGTT TGTGACGAGT GCTGCAGTGT GCACCGGAGC 
CTGGGACGAC ACATCTCCAT TGTCAAACAC CTTCGCCACA GCGCCTGGCC TCCTACGCTG 
CTACAGATGG TGCACACGCT TGCCAGCAAC GGGGCCAACT CTATCTGGGA GCATTCCCTG 
CTGGACCCTG CACAAGTGCA AAGTGGCCGG CGCAAAGCCA ACCCCCAAGA CAAAGTCCAC 
CCCATCAAGT CAGAGTTCAT CAGGGCAAAG TACCAGATGC TGGCGTTTGT GCACAAGCTT 
CCCTGCCGTG ATGATGATGG GGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCGAGT 
GTGCGAACAG GCAACCTGGA GACCTGTCTG CGCCTGCTTT CCCTGGGTGC CCAGGCCAAC 
TTCTTCCACC CAGAAAAGGG CACTACACCT CTACACGTGG CTGCCAAGGC AGGGCAGACA 
CTGCAGGCTG AGCTGCTAGT AGTGTATGGG GCTGACCCAG GCTCCCCTGA TGTCAATGGC 
CGCACACCCA TTGACTATGC CAGGCAGGCG GGGCACCATG AACTGGCAGA AAGGCTAGTT 
GAGTGCCAGT ATGAGCTCAC TGACCGGTTG GCCTTCTACC TCTGTGGACG GAAGCCTGAT 
CACAAGAATG GGCATTACAT CATACCACAG ATGGCTGACA GATCTCGGCA AAAGTGCATG 
TCTCAGAGTT TGGATCTGTC TGAATTGGCC AAAGCTGCCA AGAAGAAGCT ACAAGCACTC 
AGCAACCGGC TCTTTGAGGA GCTCGCCATG GATGTGTATG ACGAAGTGGA CCGGAGAGAA 
AATGATGCAG TGTGGCTAGC TACCCAGAAT CATAGCACCC TGGTGACAGA ACGCAGTGCT 
GTGCCCTTCC TGCCAGTCAA CCCTGAATAC TCAGCCACTC GGAATCAGGG ACGACAGAAA 
TTGGCTCGTT TTAATGCCCG AGAGTTTGCC ACCTTGATCA TCGACATCCT CAGTGAGGCC 
AAACGGAGGC AGCAGGGCAA GAGTCTGAGC AGCCCCACAG ACAACCTCGA GCTGTCTGCA 
CGGAACCAAA GTGACCTGGA CGACCAGCAC GACTACGATA GTGTGGCTTC TGACGAAGAC 
ACAGACCAGG AGCCCTTGCC CAGTGCTGGC GCCACGCGGA ACAATCGTGC CAGGAGCATG 
GACTCCTCAG ATCTGTCGGA TGGGGCTGTG ACGCTGCAGG AGTACCTGGA GCTGAAGAAG 
GCTCTGGCTA CCTCCGAGGC CAAAGTGCAG CAGCTCATGA AAGTCAACAG CAGTCTGAGT 
GACGAGCTTC GGAAGCTGCA GAGGGAGATC CACAAACTGC AGGCAGAGAA CCTGCAGCTC 
CGGCAGCCAC CAGGACCAGT GCCTGTACCC TCACTTCCCA GTGAACGGGC AGAACACACA 
CTCATGGGCC CTGGTGGAAG TACCCATCGC AGGGACCGCC AGGCCTTCTC TATGTATGAG 
CCAGGCTCCG CCCTGAAGCC CTTTGGAGGT GCACCTGGGG ATGAGCTCGC CACACGGCTC 
CAGCCTTTCC ACAGCACAGA GCTGGAAGAT GATGCCATCT ATTCAGTACA TGTCCCTGCT 
GGCCTTTACC GGATCCGGAA GGGGGTGTCT GCCTCTTCTG TGACCTTCAC TCCCTCCTCC 
CCACTGCTGT CAAGCTCCCA AGAAGGAAGT CGCCATGCGA GCAAGCTTTC ACGCCACGGC 
AGTGGCGCAG AGAGTGACTA TGAGAACACA CAGAGTGGAG AGCCTCTGCT GGGACTTGAA 
GGGAAGCGAT TCCTAGAGCT GAGCAAGGAG GATGAGCTGC ACGCTGAGCT GGAGAGCTTA 
GATGGAGACC CAGACCCCGG GCTCCCCAGC ACAGAAGATG TCATCCTAAA GACAGAGCAG 
GTCACCAAGA ACATTCAGGA GCTATTGCGG GCAGCCCAGG AGTTCAAACA TGACAGCTTT 
GTGCCCTGTT CAGAAAAGAT CCATTTGGCT GTGACTGAGA TGGCCTCTCT CTTCCCAAAG 
AGGCCAGCCC TGGAGCCTGT GCGCAGTTCA CTGCGGCTGC TCAACGCCAG CGCCTACCGG 
CTGCAGAGTG AATGCCGGAA GACAGTGCCT CCAGAACCTG GCGCCCCTGT GGACTTCCAG 
CTACTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCTGC CAAGCAGCTG 
GTCACCATCA CCACCCGAGA GAAGAAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-PIX plays important role in regulation of intestinal epithelial restitution by interacting with GIT1 and Rac1 after wounding.
American journal of physiology. Gastrointestinal and liver physiologyajpgi002962017(2017 Nov)
Rathor N,Chung HK,Wang SR,Qian M,Turner DJ,Wang JY,Rao JN

Presynaptic Deletion of GIT Proteins Results in Increased Synaptic Strength at a Mammalian Central Synapse.
Neuron88(5)918-925(2015 Dec)
Montesinos MS,Dong W,Goff K,Das B,Guerrero-Given D,Schmalzigaug R,Premont RT,Satterfield R,Kamasawa N,Young SM Jr

Integrin-?1 regulates chondrocyte proliferation and apoptosis through the upregulation of GIT1 expression.
International journal of molecular medicine35(4)1074-80(2015 Apr)
Zhang LQ,Zhao GZ,Xu XY,Fang J,Chen JM,Li JW,Gao XJ,Hao LJ,Chen YZ

PDGF regulates chondrocyte proliferation through activation of the GIT1- and PLC?1-mediated ERK1/2 signaling pathway.
Molecular medicine reports10(5)2409-14(2014 Nov)
Xiao J,Chen X,Xu L,Zhang Y,Yin Q,Wang F

GIT1 and ?PIX are essential for GABA(A) receptor synaptic stability and inhibitory neurotransmission.
Cell reports9(1)298-310(2014 Oct)
Smith KR,Davenport EC,Wei J,Li X,Pathania M,Vaccaro V,Yan Z,Kittler JT

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Git1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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