Fads2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fads2
Entrez Gene ID	83512
Full Name	fatty acid desaturase 2
Synonyms	Fadsd6
General protein information	Preferred Names fatty acid desaturase 2 Names fatty acid desaturase 2 D6D delta(6) desaturase delta-6 fatty acid desaturase fatty acid desaturase 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760244.2	XP_008758466.1	fatty acid desaturase 2 isoform X1
NM_031344.2	NP_112634.1	acyl-CoA 6-desaturase

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	fat-3	NP_001255426.1
Caenorhabditis elegans (roundworm)	fat-4	NP_001255423.1
Mus musculus (house mouse)	Fads2	NP_062673.1
Danio rerio (zebrafish)	fads2	NP_571720.2
Homo sapiens (human)	FADS2	NP_004256.1
Pan troglodytes (chimpanzee)	FADS2	XP_508482.2
Rattus norvegicus (Norway rat)	Fads2	NP_112634.1
Gallus gallus (chicken)	FADS2	NP_001153900.1
Xenopus tropicalis (tropical clawed frog)	fads2	NP_001120262.1
	FADS2	XP_001116629.1
Canis lupus familiaris (dog)	FADS2	XP_005631707.1
Bos taurus (cattle)	FADS2	NP_001076913.1

Gene Ontology
Bibliography

Related articles in PubMed

Influence of the cis-9, cis-12 and cis-15 double bond position in octadecenoic acid (18:1) isomers on the rat FADS2-catalyzed ?6-desaturation.
Rioux V, Choque B, Ezanno H, Duby C, Catheline D, Legrand P
Chemistry and physics of lipids18710-9(2015 Apr)

Role of maternal tissue in the synthesis of polyunsaturated fatty acids in response to a lipid-deficient diet during pregnancy and lactation in rats.
Gonz?lez RS, Rodriguez-Cruz M, Maldonado J, Saavedra FJ
Gene549(1)7-23(2014 Oct)

Delta-6-desaturase links polyunsaturated fatty acid metabolism with phospholipid remodeling and disease progression in heart failure.
Le CH, Mulligan CM, Routh MA, Bouma GJ, Frye MA, Jeckel KM, Sparagna GC, Lynch JM, Moore RL, McCune SA, Bristow M, Zarini S, Murphy RC, Chicco AJ
Circulation. Heart failure7(1)172-83(2014 Jan)

Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver.
Hoile SP, Irvine NA, Kelsall CJ, Sibbons C, Feunteun A, Collister A, Torrens C, Calder PC, Hanson MA, Lillycrop KA, Burdge GC
The Journal of nutritional biochemistry24(7)1213-20(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We first showed that both recombinant and native rat FADS2 were able to Delta6-desaturate not only the cis9- but also the cis12- and cis15-18:1 isomers.
Title: Influence of the cis-9, cis-12 and cis-15 double bond position in octadecenoic acid (18:1) isomers on the rat FADS2-catalyzed ?6-desaturation.

Results show that genes with the highest expression in the mammary gland, liver and adipose tissue were Elovl5, Fads2, and Fasn, respectively, in a low-lipid diet than in adequate-lipid diet.
Title: Role of maternal tissue in the synthesis of polyunsaturated fatty acids in response to a lipid-deficient diet during pregnancy and lactation in rats.

Delta-6-desaturase links polyunsaturated fatty acid metabolism with phospholipid remodeling and disease progression in heart failure.
Title: Delta-6-desaturase links polyunsaturated fatty acid metabolism with phospholipid remodeling and disease progression in heart failure.

epigenetic regulation of Fads2 may contribute to short- and long-term regulation of PUFA synthesis
Title: Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver.

Changes in progesterone and oestradiol during pregnancy may promote the synthesis of LC PUFA via increased FADS2 expression.
Title: Changes in rat n-3 and n-6 fatty acid composition during pregnancy are associated with progesterone concentrations and hepatic FADS2 expression.

The following Fads2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fads2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42798	NM_031344.2 Latest version!	Rattus norvegicus fatty acid desaturase 2 (Fads2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$258.30 $369.00
ORa43757	XM_008760244.2 Latest version!	Rattus norvegicus fatty acid desaturase 2 (Fads2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fads2 ( NM_031344.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112634.1 Rattus norvegicus fatty acid desaturase 2 (Fads2), mRNA.

Price & Availability↑

CloneID	ORa42798
Clone ID Related Accession (Same CDS sequence)	NM_031344.2
Accession Version	NM_031344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	acyl-CoA 6-desaturase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081776.1. On Sep 16, 2004 this sequence version replaced NM_031344.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081776.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAAGG GAGGTAACCA GGGAGAGGGG AGCACCGAGC TCCAGGCTCC GATGCCCACC 
TTCCGCTGGG AGGAGATTCA GAAGCACAAC CTGCGCACCG ACCGGTGGCT CGTCATCGAC 
CGCAAGGTCT ACAACGTTAC CAAATGGTCC CAGCGGCACC CAGGGGGGCA CCGTGTCATC 
GGACACTATT CGGGAGAAGA TGCTACGGAT GCCTTCCGTG CCTTCCACCT GGACCTGGAT 
TTCGTGGGCA AGTTCTTGAA GCCCCTGCTG ATTGGTGAGC TGGCCCCAGA GGAGCCCAGC 
CTGGACCGGG GCAAAAGCTC TCAGATCACC GAGGACTTCA GGGCCCTGAA GAAGACTGCT 
GAGGACATGA ACCTTTTCAA AACCAACCAC CTGTTCTTCT TTCTCCTCCT GTCCCACATC 
ATCGTCATGG AAAGCATCGC CTGGTTCATC CTCTCGTACT TCGGCAATGG CTGGATTCCC 
ACCGTCATCA CGGCCTTTGT CCTTGCTACC TCCCAGGCCC AAGCTGGATG GCTACAACAT 
GATTATGGCC ACCTTTCTGT CTATAAGAAA TCCATATGGA ACCACATTGT CCACAAGTTT 
GTCATTGGCC ACTTAAAGGG TGCCTCCGCC AACTGGTGGA ACCATCGACA TTTCCAGCAC 
CATGCGAAGC CCAACATCTT CCACAAGGAC CCCGACATAA AGAGCCTGCA CGTGTTTGTC 
CTTGGAGAGT GGCAGCCCCT CGAGTATGGC AAGAAGAAGC TGAAATATCT GCCCTACAAC 
CACCAGCATG AATACTTCTT CCTGATCGGA CCACCGCTGC TCATCCCTAT GTACTTCCAG 
TACCAGATCA TCATGACCAT GATCAGACGC AGAGACTGGG TGGACTTGGC CTGGGCCATC 
AGCTACTATG CACGTTTCTT CTACACCTAT ATCCCTTTCT ATGGCATCTT GGGAGCCCTG 
GTTTTCCTCA ACTTTATCAG GTTCCTGGAG AGCCACTGGT TTGTGTGGGT CACACAGATG 
AACCACATTG TCATGGAGAT TGATCTTGAT CACTACCGGG ACTGGTTCAG CAGCCAGCTG 
GCAGCCACCT GCAATGTGGA GCAGTCCTTC TTCAATGACT GGTTCAGCGG GCACCTCAAT 
TTCCAGATTG AGCACCACCT CTTCCCCACT ATGCCAAGAC ACAACTTGCA CAAGATTGCC 
CCACTGGTGA AGTCTCTCTG CGCCAAGCAT GGCATTGAAT ACCAAGAGAA GCCGCTGCTG 
AGGGCCCTGC TCGACATTGT GAGTTCACTG AAGAAGTCTG GGGAGCTGTG GCTGGATGCC 
TACCTCCACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112634.1
CDS	167..1501
Translation	MGKGGNQGEGSTELQAPMPTFRWEEIQKHNLRTDRWLVIDRKVYNVTKWSQRHPGGHRVIGHYSGEDATDAFRAFHLDLDFVGKFLKPLLIGELAPEEPSLDRGKSSQITEDFRALKKTAEDMNLFKTNHLFFFLLLSHIIVMESIAWFILSYFGNGWIPTVITAFVLATSQAQAGWLQHDYGHLSVYKKSIWNHIVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDIKSLHVFVLGEWQPLEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIRRRDWVDLAWAISYYARFFYTYIPFYGILGALVFLNFIRFLESHWFVWVTQMNHIVMEIDLDHYRDWFSSQLAATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIVSSLKKSGELWLDAYLHK

Target ORF information:

RefSeq Version	NM_031344.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fatty acid desaturase 2 (Fads2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031344.2

ORF Insert Sequence:

ATGGGGAAGG GAGGTAACCA GGGAGAGGGG AGCACCGAGC TCCAGGCTCC GATGCCCACC 
TTCCGCTGGG AGGAGATTCA GAAGCACAAC CTGCGCACCG ACCGGTGGCT CGTCATCGAC 
CGCAAGGTCT ACAACGTTAC CAAATGGTCC CAGCGGCACC CAGGGGGGCA CCGTGTCATC 
GGACACTATT CGGGAGAAGA TGCTACGGAT GCCTTCCGTG CCTTCCACCT GGACCTGGAT 
TTCGTGGGCA AGTTCTTGAA GCCCCTGCTG ATTGGTGAGC TGGCCCCAGA GGAGCCCAGC 
CTGGACCGGG GCAAAAGCTC TCAGATCACC GAGGACTTCA GGGCCCTGAA GAAGACTGCT 
GAGGACATGA ACCTTTTCAA AACCAACCAC CTGTTCTTCT TTCTCCTCCT GTCCCACATC 
ATCGTCATGG AAAGCATCGC CTGGTTCATC CTCTCGTACT TCGGCAATGG CTGGATTCCC 
ACCGTCATCA CGGCCTTTGT CCTTGCTACC TCCCAGGCCC AAGCTGGATG GCTACAACAT 
GATTATGGCC ACCTTTCTGT CTATAAGAAA TCCATATGGA ACCACATTGT CCACAAGTTT 
GTCATTGGCC ACTTAAAGGG TGCCTCCGCC AACTGGTGGA ACCATCGACA TTTCCAGCAC 
CATGCGAAGC CCAACATCTT CCACAAGGAC CCCGACATAA AGAGCCTGCA CGTGTTTGTC 
CTTGGAGAGT GGCAGCCCCT CGAGTATGGC AAGAAGAAGC TGAAATATCT GCCCTACAAC 
CACCAGCATG AATACTTCTT CCTGATCGGA CCACCGCTGC TCATCCCTAT GTACTTCCAG 
TACCAGATCA TCATGACCAT GATCAGACGC AGAGACTGGG TGGACTTGGC CTGGGCCATC 
AGCTACTATG CACGTTTCTT CTACACCTAT ATCCCTTTCT ATGGCATCTT GGGAGCCCTG 
GTTTTCCTCA ACTTTATCAG GTTCCTGGAG AGCCACTGGT TTGTGTGGGT CACACAGATG 
AACCACATTG TCATGGAGAT TGATCTTGAT CACTACCGGG ACTGGTTCAG CAGCCAGCTG 
GCAGCCACCT GCAATGTGGA GCAGTCCTTC TTCAATGACT GGTTCAGCGG GCACCTCAAT 
TTCCAGATTG AGCACCACCT CTTCCCCACT ATGCCAAGAC ACAACTTGCA CAAGATTGCC 
CCACTGGTGA AGTCTCTCTG CGCCAAGCAT GGCATTGAAT ACCAAGAGAA GCCGCTGCTG 
AGGGCCCTGC TCGACATTGT GAGTTCACTG AAGAAGTCTG GGGAGCTGTG GCTGGATGCC 
TACCTCCACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fads2 ( XM_008760244.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758466.1 Rattus norvegicus fatty acid desaturase 2 (Fads2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43757
Clone ID Related Accession (Same CDS sequence)	XM_008760244.2
Accession Version	XM_008760244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	fatty acid desaturase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGAG AGGATGCCTT CCGTGCCTTC CACCTGGACC TGGATTTCGT GGGCAAGTTC 
TTGAAGCCCC TGCTGATTGG TGAGCTGGCC CCAGAGGAGC CCAGCCTGGA CCGGGGCAAA 
AGCTCTCAGA TCACCGAGGA CTTCAGGGCC CTGAAGAAGA CTGCTGAGGA CATGAACCTT 
TTCAAAACCA ACCACCTGTT CTTCTTTCTC CTCCTGTCCC ACATCATCGT CATGGAAAGC 
ATCGCCTGGT TCATCCTCTC GTACTTCGGC AATGGCTGGA TTCCCACCGT CATCACGGCC 
TTTGTCCTTG CTACCTCCCA GGCCCAAGCT GGATGGCTAC AACATGATTA TGGCCACCTT 
TCTGTCTATA AGAAATCCAT ATGGAACCAC ATTGTCCACA AGTTTGTCAT TGGCCACTTA 
AAGGGTGCCT CCGCCAACTG GTGGAACCAT CGACATTTCC AGCACCATGC GAAGCCCAAC 
ATCTTCCACA AGGACCCCGA CATAAAGAGC CTGCACGTGT TTGTCCTTGG AGAGTGGCAG 
CCCCTCGAGT ATGGCAAGAA GAAGCTGAAA TATCTGCCCT ACAACCACCA GCATGAATAC 
TTCTTCCTGA TCGGACCACC GCTGCTCATC CCTATGTACT TCCAGTACCA GATCATCATG 
ACCATGATCA GACGCAGAGA CTGGGTGGAC TTGGCCTGGG CCATCAGCTA CTATGCACGT 
TTCTTCTACA CCTATATCCC TTTCTATGGC ATCTTGGGAG CCCTGGTTTT CCTCAACTTT 
ATCAGGTTCC TGGAGAGCCA CTGGTTTGTG TGGGTCACAC AGATGAACCA CATTGTCATG 
GAGATTGATC TTGATCACTA CCGGGACTGG TTCAGCAGCC AGCTGGCAGC CACCTGCAAT 
GTGGAGCAGT CCTTCTTCAA TGACTGGTTC AGCGGGCACC TCAATTTCCA GATTGAGCAC 
CACCTCTTCC CCACTATGCC AAGACACAAC TTGCACAAGA TTGCCCCACT GGTGAAGTCT 
CTCTGCGCCA AGCATGGCAT TGAATACCAA GAGAAGCCGC TGCTGAGGGC CCTGCTCGAC 
ATTGTGAGTT CACTGAAGAA GTCTGGGGAG CTGTGGCTGG ATGCCTACCT CCACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758466.1
CDS	1869..3008
Translation	MTGEDAFRAFHLDLDFVGKFLKPLLIGELAPEEPSLDRGKSSQITEDFRALKKTAEDMNLFKTNHLFFFLLLSHIIVMESIAWFILSYFGNGWIPTVITAFVLATSQAQAGWLQHDYGHLSVYKKSIWNHIVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDIKSLHVFVLGEWQPLEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIRRRDWVDLAWAISYYARFFYTYIPFYGILGALVFLNFIRFLESHWFVWVTQMNHIVMEIDLDHYRDWFSSQLAATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIVSSLKKSGELWLDAYLHK

Target ORF information:

RefSeq Version	XM_008760244.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fatty acid desaturase 2 (Fads2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760244.2

ORF Insert Sequence:

ATGACAGGAG AGGATGCCTT CCGTGCCTTC CACCTGGACC TGGATTTCGT GGGCAAGTTC 
TTGAAGCCCC TGCTGATTGG TGAGCTGGCC CCAGAGGAGC CCAGCCTGGA CCGGGGCAAA 
AGCTCTCAGA TCACCGAGGA CTTCAGGGCC CTGAAGAAGA CTGCTGAGGA CATGAACCTT 
TTCAAAACCA ACCACCTGTT CTTCTTTCTC CTCCTGTCCC ACATCATCGT CATGGAAAGC 
ATCGCCTGGT TCATCCTCTC GTACTTCGGC AATGGCTGGA TTCCCACCGT CATCACGGCC 
TTTGTCCTTG CTACCTCCCA GGCCCAAGCT GGATGGCTAC AACATGATTA TGGCCACCTT 
TCTGTCTATA AGAAATCCAT ATGGAACCAC ATTGTCCACA AGTTTGTCAT TGGCCACTTA 
AAGGGTGCCT CCGCCAACTG GTGGAACCAT CGACATTTCC AGCACCATGC GAAGCCCAAC 
ATCTTCCACA AGGACCCCGA CATAAAGAGC CTGCACGTGT TTGTCCTTGG AGAGTGGCAG 
CCCCTCGAGT ATGGCAAGAA GAAGCTGAAA TATCTGCCCT ACAACCACCA GCATGAATAC 
TTCTTCCTGA TCGGACCACC GCTGCTCATC CCTATGTACT TCCAGTACCA GATCATCATG 
ACCATGATCA GACGCAGAGA CTGGGTGGAC TTGGCCTGGG CCATCAGCTA CTATGCACGT 
TTCTTCTACA CCTATATCCC TTTCTATGGC ATCTTGGGAG CCCTGGTTTT CCTCAACTTT 
ATCAGGTTCC TGGAGAGCCA CTGGTTTGTG TGGGTCACAC AGATGAACCA CATTGTCATG 
GAGATTGATC TTGATCACTA CCGGGACTGG TTCAGCAGCC AGCTGGCAGC CACCTGCAAT 
GTGGAGCAGT CCTTCTTCAA TGACTGGTTC AGCGGGCACC TCAATTTCCA GATTGAGCAC 
CACCTCTTCC CCACTATGCC AAGACACAAC TTGCACAAGA TTGCCCCACT GGTGAAGTCT 
CTCTGCGCCA AGCATGGCAT TGAATACCAA GAGAAGCCGC TGCTGAGGGC CCTGCTCGAC 
ATTGTGAGTT CACTGAAGAA GTCTGGGGAG CTGTGGCTGG ATGCCTACCT CCACAAATGA