Dynlt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dynlt1
Entrez Gene ID	83462
Full Name	dynein light chain Tctex-type 1
Synonyms	AGS2,Tctex1
General protein information	Preferred Names dynein light chain Tctex-type 1 Names dynein light chain Tctex-type 1 T-complex testis-specific protein 1 homolog activator of G-protein signaling 2 t-complex testis expressed 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q11
Summary	mediates vesicle transport from early to late endosomes in cooperation with dynein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031318.1	NP_112608.1	dynein light chain Tctex-type 1
XM_006227901.3	XP_006227963.1	dynein light chain Tctex-type 1 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Dlc90F	NP_477356.1
Homo sapiens (human)	DYNLT1	NP_006510.1
Bos taurus (cattle)	DYNLT1	NP_777045.1
Mus musculus (house mouse)	Dynlt1f	NP_001160099.1
Mus musculus (house mouse)	Dynlt1a	NP_001160101.1
Danio rerio (zebrafish)	dynlt1	NP_001129255.1
Pan troglodytes (chimpanzee)	DYNLT1	XP_001147088.1
Gallus gallus (chicken)	DYNLT1	XP_001232357.1
Mus musculus (house mouse)	Dynlt1c	NP_001160102.1
Xenopus tropicalis (tropical clawed frog)	dynlt1	NP_001011428.1
Canis lupus familiaris (dog)	DYNLT1	XP_003638830.1
Mus musculus (house mouse)	Dynlt1b	NP_033368.1
Rattus norvegicus (Norway rat)	Dynlt1	NP_112608.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001229	XP_321929.3

Gene Ontology
Bibliography

Related articles in PubMed

Subunit composition of the human cytoplasmic dynein-2 complex.
Asante D, Stevenson NL, Stephens DJ
Journal of cell science127(Pt 21)4774-87(2014 Nov)

Metabotropic glutamate receptor 5 knockout promotes motor and biochemical alterations in a mouse model of Huntington's disease.
Ribeiro FM, Devries RA, Hamilton A, Guimaraes IM, Cregan SP, Pires RG, Ferguson SS
Human molecular genetics23(8)2030-42(2014 Apr)

Phosphorylation of DYNLT1 at serine 82 regulates microtubule stability and mitochondrial permeabilization in hypoxia.
Xu X, Zhang Q, Hu JY, Zhang DX, Jiang XP, Jia JZ, Zhu JC, Huang YS
Molecules and cells36(4)322-32(2013 Oct)

Cytoplasmic dynein.
Allan VJ
Biochemical Society transactions39(5)1169-78(2011 Oct)

MAP4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and DYNLT1 interaction with VDAC1.
Fang YD, Xu X, Dang YM, Zhang YM, Zhang JP, Hu JY, Zhang Q, Dai X, Teng M, Zhang DX, Huang YS
PloS one6(12)e28052(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that DYNLT1 phosphorylation at serine S82 is involved in microtubule and mitochondria regulation, and their interaction and cooperation contribute to the cellular hypoxic tolerance.
Title: Phosphorylation of DYNLT1 at serine 82 regulates microtubule stability and mitochondrial permeabilization in hypoxia.

there are two possible mechanisms triggered by MAP4: stabilization of MT networks; DYNLT1 modulation, which is connected with VDAC1, and inhibition of hypoxia-induced mitochondrial permeabilization
Title: MAP4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and DYNLT1 interaction with VDAC1.

The selective high level of Tctex-1 at the growth cone drives fast neurite extension by modulating actin dynamics
Title: The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth.

The following Dynlt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dynlt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13467	NM_031318.1 Latest version!	Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa22153	XM_006227901.3 Latest version!	Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dynlt1 ( NM_031318.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112608.1 Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), mRNA.

Price & Availability↑

CloneID	ORa13467
Clone ID Related Accession (Same CDS sequence)	NM_031318.1
Accession Version	NM_031318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	dynein light chain Tctex-type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ131437.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ131437.1, BC166879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349, SAMN01906350 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGACT TCCAGGCCTC CGAGGAGACT GCATTTGTTG TGGATGAAGT GAGCAACATT 
GTAAAGGAGG CTATAGAAAG TGCCATCGGC GGTAACGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTTGT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAAACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAATG GTGCTGGGTT ACACACCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACGGAC GGGAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TGCCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112608.1
CDS	15..356
Translation	MEDFQASEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI

Target ORF information:

RefSeq Version	NM_031318.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031318.1

ORF Insert Sequence:

ATGGAAGACT TCCAGGCCTC CGAGGAGACT GCATTTGTTG TGGATGAAGT GAGCAACATT 
GTAAAGGAGG CTATAGAAAG TGCCATCGGC GGTAACGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTTGT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAAACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAATG GTGCTGGGTT ACACACCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACGGAC GGGAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TGCCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dynlt1 ( XM_006227901.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227963.1 Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22153
Clone ID Related Accession (Same CDS sequence)	XM_006227901.3
Accession Version	XM_006227901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dynein light chain Tctex-type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT TCCAGGCCTC CGAGGAGACT GCATTTGTTG TGGATGAAGT GAGCAACATT 
GTAAAGGAGG CTATAGAAAG TGCCATCGGC GGTAACGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTTGT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAAACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAATG GTGCTGGGTT ACACACCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACGGAC GGTAGGGTTC TTACCCCCAT GCCCACTGCT 
CTGGAACTTT CACATAGCTC CGTGGTTCAG AGATCTGCAG TTCGAAGCAG TGTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227963.1
CDS	83..442
Translation	MEDFQASEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDGRVLTPMPTALELSHSSVVQRSAVRSSVL

Target ORF information:

RefSeq Version	XM_006227901.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein light chain Tctex-type 1 (Dynlt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227901.3

ORF Insert Sequence:

ATGGAAGACT TCCAGGCCTC CGAGGAGACT GCATTTGTTG TGGATGAAGT GAGCAACATT 
GTAAAGGAGG CTATAGAAAG TGCCATCGGC GGTAACGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTTGT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAAACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAATG GTGCTGGGTT ACACACCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACGGAC GGTAGGGTTC TTACCCCCAT GCCCACTGCT 
CTGGAACTTT CACATAGCTC CGTGGTTCAG AGATCTGCAG TTCGAAGCAG TGTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Subunit composition of the human cytoplasmic dynein-2 complex.
Journal of cell science127(Pt 21)4774-87(2014 Nov)
Asante D,Stevenson NL,Stephens DJ

Metabotropic glutamate receptor 5 knockout promotes motor and biochemical alterations in a mouse model of Huntington's disease.
Human molecular genetics23(8)2030-42(2014 Apr)
Ribeiro FM,Devries RA,Hamilton A,Guimaraes IM,Cregan SP,Pires RG,Ferguson SS

Phosphorylation of DYNLT1 at serine 82 regulates microtubule stability and mitochondrial permeabilization in hypoxia.
Molecules and cells36(4)322-32(2013 Oct)
Xu X,Zhang Q,Hu JY,Zhang DX,Jiang XP,Jia JZ,Zhu JC,Huang YS

Cytoplasmic dynein.
Biochemical Society transactions39(5)1169-78(2011 Oct)
Allan VJ

MAP4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and DYNLT1 interaction with VDAC1.
PloS one6(12)e28052(2011)
Fang YD,Xu X,Dang YM,Zhang YM,Zhang JP,Hu JY,Zhang Q,Dai X,Teng M,Zhang DX,Huang YS