Psmc5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psmc5
Entrez Gene ID	81827
Full Name	proteasome 26S subunit, ATPase 5
Synonyms	Sug1
General protein information	Preferred Names proteasome 26S subunit, ATPase 5 Names 26S proteasome regulatory subunit 8 26S protease regulatory subunit 8 26S proteasome AAA-ATPase subunit RPT6 TRIP1 for proteasomal ATPase (SUG1) p45/SUG peptidase (prosome, macropain) 26S subunit, ATPase 5 protease (prosome, macropain) 26S subunit, ATPase 5 proteasomal ATPase (SUG1) proteasome (prosome, macropain) 26S subunit, ATPase, 5 proteasome p45/SUG proteasome subunit p45 thyroid hormone receptor-interacting protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	may play a role in ATP-dependent RNA/DNA unwinding [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031149.1	NP_112411.1	26S proteasome regulatory subunit 8

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pros45	NP_608447.1
Saccharomyces cerevisiae (baker's yeast)	RPT6	NP_011467.1
Eremothecium gossypii	AGOS_AGL043C	NP_986623.1
Homo sapiens (human)	PSMC5	NP_002796.4
Pan troglodytes (chimpanzee)	PSMC5	NP_001233453.1
	PSMC5	XP_001108909.2
Mus musculus (house mouse)	Psmc5	NP_032976.1
Schizosaccharomyces pombe (fission yeast)	rpt6	NP_595870.1
Arabidopsis thaliana (thale cress)	AT5G20000	NP_197500.1
Oryza sativa (rice)	Os02g0205300	NP_001046248.1
Xenopus tropicalis (tropical clawed frog)	psmc5	NP_989358.1
Bos taurus (cattle)	PSMC5	NP_776866.1
Caenorhabditis elegans (roundworm)	rpt-6	NP_499609.1
Neurospora crassa	NCU05363	XP_963354.1
Rattus norvegicus (Norway rat)	Psmc5	NP_112411.1
Gallus gallus (chicken)	PSMC5	XP_004948567.1
Danio rerio (zebrafish)	psmc5	NP_001003740.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007243	XP_308557.2
Magnaporthe oryzae (rice blast fungus)	MGG_01380	XP_003714319.1
Canis lupus familiaris (dog)	PSMC5	XP_005624306.1
Caenorhabditis elegans (roundworm)	F56F11.4	NP_741098.1
Kluyveromyces lactis	KLLA0A04752g	XP_451208.1
Arabidopsis thaliana (thale cress)	RPT6A	NP_568389.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

Phosphorylation of Rpt6 regulates synaptic strength in hippocampal neurons.
Djakovic SN, Marquez-Lona EM, Jakawich SK, Wright R, Chu C, Sutton MA, Patrick GN
The Journal of neuroscience : the official journal of the Society for Neuroscience32(15)5126-31(2012 Apr)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Sasaki K, Hamazaki J, Koike M, Hirano Y, Komatsu M, Uchiyama Y, Tanaka K, Murata S
Molecular and cellular biology30(15)3864-74(2010 Aug)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CaMKII-dependent phosphorylation of Rpt6 at S120 may be an important regulatory mechanism for proteasome-dependent control of synaptic remodeling in slow homeostatic plasticity
Title: Phosphorylation of Rpt6 regulates synaptic strength in hippocampal neurons.

cyclic AMP-dependent protein kinase regulates proteasome function through phosphorylation of Rpt6
Title: Proteasome function is regulated by cyclic AMP-dependent protein kinase through phosphorylation of Rpt6.

The following Psmc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psmc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07756	NM_031149.1 Latest version!	Rattus norvegicus proteasome 26S subunit, ATPase 5 (Psmc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psmc5 ( NM_031149.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112411.1 Rattus norvegicus proteasome 26S subunit, ATPase 5 (Psmc5), mRNA.

Price & Availability↑

CloneID	ORa07756
Clone ID Related Accession (Same CDS sequence)	NM_031149.1
Accession Version	NM_031149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	26S proteasome regulatory subunit 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB000491.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB000491.1, FQ213263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTTG ATGGGCCAGA GCAGATGGAA CTGGAAGAGG GGAAGGCAGG CAGTGGACTC 
CGTCAATATT ATCTGTCCAA GATTGAAGAA CTCCAGTTGA TTGTGAATGA CAAGAGTCAG 
AACCTCCGGA GACTGCAGGC CCAGAGGAAT GAGCTCAATG CAAAAGTTCG TCTGTTGCGG 
GAGGAACTGC AGCTGCTGCA GGAACAGGGC TCCTACGTTG GAGAAGTCGT GAGGGCCATG 
GATAAGAAAA AAGTGTTGGT CAAGGTCCAT CCTGAGGGCA AATTTGTTGT TGATGTGGAC 
AAGAACATTG ATATCAATGA TGTGACACCC AATTGTCGGG TGGCTCTAAG AAATGACAGC 
TACACTCTGC ATAAGATCCT GCCTAACAAA GTGGACCCTT TGGTATCACT AATGATGGTG 
GAAAAGGTGC CAGACTCAAC CTACGAGATG ATTGGCGGGC TAGACAAGCA GATCAAGGAG 
ATCAAAGAAG TGATTGAGCT GCCTGTGAAG CACCCTGAGC TCTTTGAAGC CCTGGGCATT 
GCACAGCCAA AGGGAGTCCT GCTATATGGA CCCCCGGGCA CTGGGAAGAC GTTGTTGGCC 
CGGGCTGTGG CTCATCATAC GGACTGTACC TTTATTCGTG TCTCTGGCTC TGAACTGGTA 
CAGAAATTCA TTGGGGAAGG GGCAAGAATG GTGAGGGAGC TGTTTGTCAT GGCCCGAGAA 
CATGCTCCCT CCATCATCTT CATGGACGAG ATCGACTCTA TCGGTTCTTC ACGGCTGGAG 
GGAGGCTCTG GAGGCGACAG TGAGGTACAG CGCACAATGC TGGAACTGCT CAACCAGCTG 
GATGGCTTTG AGGCCACTAA GAATATCAAG GTTATCATGG CTACTAATAG GATTGATATC 
CTGGACTCTG CCCTGCTTCG TCCTGGGAGG ATTGACAGGA AAATTGAATT CCCACCTCCC 
AATGAGGAGG CCCGGCTGGA CATTTTGAAA ATCCACTCTC GGAAAATGAA CTTGACCAGG 
GGGATCAACC TGAGGAAAAT TGCTGAGTTG ATGCCAGGAG CTTCAGGGGC TGAAGTGAAG 
GGCGTGTGCA CAGAGGCCGG CATGTATGCT CTGCGGGAAC GGCGTGTCCA CGTTACTCAG 
GAGGACTTTG AGATGGCAGT AGCCAAGGTC ATGCAAAAGG ATAGTGAGAA AAACATGTCC 
ATCAAGAAGC TTTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112411.1
CDS	16..1236
Translation	MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK

Target ORF information:

RefSeq Version	NM_031149.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proteasome 26S subunit, ATPase 5 (Psmc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031149.1

ORF Insert Sequence:

ATGGCGCTTG ATGGGCCAGA GCAGATGGAA CTGGAAGAGG GGAAGGCAGG CAGTGGACTC 
CGTCAATATT ATCTGTCCAA GATTGAAGAA CTCCAGTTGA TTGTGAATGA CAAGAGTCAG 
AACCTCCGGA GACTGCAGGC CCAGAGGAAT GAGCTCAATG CAAAAGTTCG TCTGTTGCGG 
GAGGAACTGC AGCTGCTGCA GGAACAGGGC TCCTACGTTG GAGAAGTCGT GAGGGCCATG 
GATAAGAAAA AAGTGTTGGT CAAGGTCCAT CCTGAGGGCA AATTTGTTGT TGATGTGGAC 
AAGAACATTG ATATCAATGA TGTGACACCC AATTGTCGGG TGGCTCTAAG AAATGACAGC 
TACACTCTGC ATAAGATCCT GCCTAACAAA GTGGACCCTT TGGTATCACT AATGATGGTG 
GAAAAGGTGC CAGACTCAAC CTACGAGATG ATTGGCGGGC TAGACAAGCA GATCAAGGAG 
ATCAAAGAAG TGATTGAGCT GCCTGTGAAG CACCCTGAGC TCTTTGAAGC CCTGGGCATT 
GCACAGCCAA AGGGAGTCCT GCTATATGGA CCCCCGGGCA CTGGGAAGAC GTTGTTGGCC 
CGGGCTGTGG CTCATCATAC GGACTGTACC TTTATTCGTG TCTCTGGCTC TGAACTGGTA 
CAGAAATTCA TTGGGGAAGG GGCAAGAATG GTGAGGGAGC TGTTTGTCAT GGCCCGAGAA 
CATGCTCCCT CCATCATCTT CATGGACGAG ATCGACTCTA TCGGTTCTTC ACGGCTGGAG 
GGAGGCTCTG GAGGCGACAG TGAGGTACAG CGCACAATGC TGGAACTGCT CAACCAGCTG 
GATGGCTTTG AGGCCACTAA GAATATCAAG GTTATCATGG CTACTAATAG GATTGATATC 
CTGGACTCTG CCCTGCTTCG TCCTGGGAGG ATTGACAGGA AAATTGAATT CCCACCTCCC 
AATGAGGAGG CCCGGCTGGA CATTTTGAAA ATCCACTCTC GGAAAATGAA CTTGACCAGG 
GGGATCAACC TGAGGAAAAT TGCTGAGTTG ATGCCAGGAG CTTCAGGGGC TGAAGTGAAG 
GGCGTGTGCA CAGAGGCCGG CATGTATGCT CTGCGGGAAC GGCGTGTCCA CGTTACTCAG 
GAGGACTTTG AGATGGCAGT AGCCAAGGTC ATGCAAAAGG ATAGTGAGAA AAACATGTCC 
ATCAAGAAGC TTTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Journal of proteomics75(12)3574-84(2012 Jun)
Bastos-Amador P,Royo F,Gonzalez E,Conde-Vancells J,Palomo-Diez L,Borras FE,Falcon-Perez JM

Phosphorylation of Rpt6 regulates synaptic strength in hippocampal neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(15)5126-31(2012 Apr)
Djakovic SN,Marquez-Lona EM,Jakawich SK,Wright R,Chu C,Sutton MA,Patrick GN

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Molecular and cellular biology30(15)3864-74(2010 Aug)
Sasaki K,Hamazaki J,Koike M,Hirano Y,Komatsu M,Uchiyama Y,Tanaka K,Murata S

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA