Gpr12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr12
Entrez Gene ID	80840
Full Name	G protein-coupled receptor 12
Synonyms	Gpcr12
General protein information	Preferred Names G protein-coupled receptor 12 Names G-protein coupled receptor 12 R334
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	orphan G-protein coupled receptor expressed in brain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598463.1	XP_017453952.1	G-protein coupled receptor 12 isoform X1
NM_001037295.1	NP_001032372.1	G-protein coupled receptor 12
XM_017598464.1	XP_017453953.1	G-protein coupled receptor 12 isoform X2
NM_030831.1	NP_110458.1	G-protein coupled receptor 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR12	NP_005279.1
Rattus norvegicus (Norway rat)	Gpr12	NP_001032372.1
Gallus gallus (chicken)	GPR12	XP_004938835.1
Xenopus tropicalis (tropical clawed frog)	gpr12	XP_002941580.1
	GPR12	XP_001094268.1
Mus musculus (house mouse)	Gpr12	NP_032177.1
Pan troglodytes (chimpanzee)	GPR12	XP_001157503.1
Canis lupus familiaris (dog)	GPR12	XP_005635581.1
Bos taurus (cattle)	GPR12	NP_001193259.1
Danio rerio (zebrafish)	gpr12	XP_005162795.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of GPR12 in the induction of neurite outgrowth in PC12 cells.
Lu X, Zhang N, Dong S, Hu Y
Brain research bulletin87(1)30-6(2012 Jan)

The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.
Hinckley M, Vaccari S, Horner K, Chen R, Conti M
Developmental biology287(2)249-61(2005 Nov)

Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.
Ignatov A, Lintzel J, Hermans-Borgmeyer I, Kreienkamp HJ, Joost P, Thomsen S, Methner A, Schaller HC
The Journal of neuroscience : the official journal of the Society for Neuroscience23(3)907-14(2003 Feb)

Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors.
Song ZH, Modi W, Bonner TI
Genomics28(2)347-9(1995 Jul)

Molecular cloning of a novel putative G protein-coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous system.
Saeki Y, Ueno S, Mizuno R, Nishimura T, Fujimura H, Nagai Y, Yanagihara T
FEBS letters336(2)317-22(1993 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that GPR12 may play a role in neurite outgrowth during PC12 cell differentiation.
Title: Involvement of GPR12 in the induction of neurite outgrowth in PC12 cells.

the cAMP levels required for maintaining meiotic arrest in mouse and rat oocytes are dependent on the expression of Gpr3 and/or Gpr12
Title: The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.

The following Gpr12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09270	NM_001037295.1 Latest version!	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09270	NM_030831.1 Latest version!	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09270	XM_017598464.1 Latest version!	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa57899	XM_017598463.1 Latest version!	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr12 ( NM_001037295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001032372.1 Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa09270
Clone ID Related Accession (Same CDS sequence)	NM_001037295.1 , XM_017598464.1 , NM_030831.1
Accession Version	NM_001037295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X61496.1 and U12184.1. ##Evidence-Data-START## Transcript exon combination :: X61496.1, BC127447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032372.1
CDS	211..1215
Translation	MNEDPKVNLSGLPRDCIEAGTPENISAAVPSQGSVVESEPELVVNPWDIVLCSSGTLICCENAVVVLIIFHSPSLRAPMFLLIGSLALADLLAGLGLIINFVFAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVMLVMLWGTSTCLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSISFLFMFALMLQLYIQICKIVMRHAHQIALQHHFLATSHYVTTRKGISTLALILGTFAACWMPFTLYSLIADYTYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPNTLSQRARSPSDV

Target ORF information:

RefSeq Version	NM_001037295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037295.1

ORF Insert Sequence:

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr12 ( NM_030831.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_110458.1 Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa09270
Clone ID Related Accession (Same CDS sequence)	NM_001037295.1 , XM_017598464.1 , NM_030831.1
Accession Version	NM_030831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U12184.1. ##Evidence-Data-START## Transcript exon combination :: U12184.1 [ECO:0000332] ##Evidence-Data-END##

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110458.1
CDS	381..1385
Translation	MNEDPKVNLSGLPRDCIEAGTPENISAAVPSQGSVVESEPELVVNPWDIVLCSSGTLICCENAVVVLIIFHSPSLRAPMFLLIGSLALADLLAGLGLIINFVFAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVMLVMLWGTSTCLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSISFLFMFALMLQLYIQICKIVMRHAHQIALQHHFLATSHYVTTRKGISTLALILGTFAACWMPFTLYSLIADYTYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPNTLSQRARSPSDV

Target ORF information:

RefSeq Version	NM_030831.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030831.1

ORF Insert Sequence:

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr12 ( XM_017598464.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453953.1 Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09270
Clone ID Related Accession (Same CDS sequence)	NM_001037295.1 , XM_017598464.1 , NM_030831.1
Accession Version	XM_017598464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453953.1
CDS	1164..2168
Translation	MNEDPKVNLSGLPRDCIEAGTPENISAAVPSQGSVVESEPELVVNPWDIVLCSSGTLICCENAVVVLIIFHSPSLRAPMFLLIGSLALADLLAGLGLIINFVFAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVMLVMLWGTSTCLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSISFLFMFALMLQLYIQICKIVMRHAHQIALQHHFLATSHYVTTRKGISTLALILGTFAACWMPFTLYSLIADYTYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPNTLSQRARSPSDV

Target ORF information:

RefSeq Version	XM_017598464.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598464.1

ORF Insert Sequence:

ATGAACGAAG ACCCGAAGGT CAATTTAAGC GGGCTGCCTC GGGACTGTAT AGAAGCTGGT 
ACTCCGGAGA ACATCTCAGC CGCTGTCCCC TCCCAGGGCT CTGTTGTGGA GTCAGAACCC 
GAGCTCGTTG TCAACCCCTG GGACATTGTC TTGTGCAGCT CAGGAACCCT CATCTGCTGT 
GAAAATGCCG TCGTGGTCCT TATCATCTTC CACAGCCCCA GCCTGCGAGC ACCCATGTTC 
CTGCTGATAG GCAGCCTGGC TCTTGCAGAC CTGCTGGCTG GTCTGGGACT CATCATCAAT 
TTTGTTTTTG CCTACCTGCT TCAGTCAGAA GCCACCAAGC TGGTCACAAT TGGACTCATT 
GTCGCCTCTT TCTCTGCCTC TGTCTGCAGT TTGCTGGCTA TCACTGTGGA CCGCTACCTC 
TCGCTGTATT ACGCCCTGAC GTACCACTCC GAGAGGACCG TCACCTTTAC CTATGTCATG 
CTAGTGATGC TCTGGGGAAC TTCCACCTGC CTGGGGCTGC TGCCTGTCAT GGGCTGGAAC 
TGCCTGAGGG ACGAGTCCAC CTGCAGCGTG GTCAGACCCC TCACTAAGAA CAACGCCGCC 
ATTCTCTCCA TCTCCTTCCT CTTCATGTTC GCACTGATGC TCCAACTCTA CATCCAGATT 
TGTAAGATTG TGATGAGGCA CGCCCATCAG ATAGCCCTGC AGCACCACTT CCTGGCTACG 
TCGCACTACG TGACTACCCG GAAAGGGATC TCCACCCTGG CTCTCATCCT GGGGACCTTT 
GCCGCCTGCT GGATGCCTTT TACCCTCTAT TCCTTGATCG CCGATTACAC CTACCCCTCC 
ATCTACACCT ACGCCACCCT CCTGCCCGCC ACGTACAATT CCATCATCAA CCCTGTCATA 
TATGCTTTCA GAAACCAAGA GATCCAGAAA GCCCTCTGCC TCATCTGCTG TGGGTGCATC 
CCTAACACGC TGTCCCAGAG AGCACGCTCT CCCAGCGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr12 ( XM_017598463.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453952.1 Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57899
Clone ID Related Accession (Same CDS sequence)	XM_017598463.1
Accession Version	XM_017598463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCG CAGGCGGGCG TCCACGGTGG CGGGCTCCCG CCCCTCACGC AGCTGCGACC 
TGCGGGGGCG CGCGCAGCCT CGTGGGGTTC TCGCGGATGC GCGCCGGGCG GGGAGCGCGC 
AGGGCGGAGA GCCGGGCGCG AGCGACGCAG CTCACCTGCC GCGGGCGCCA CCACGGACGT 
GCCACGCGGG TGGCCCTCGC TATTCGGCAG CACCGAAGGA GCCCCCCCTC GGTCTGGGCG 
TGCCAAGGGG TTAAAATGAA CGAAGACCCG AAGGTCAATT TAAGCGGGCT GCCTCGGGAC 
TGTATAGAAG CTGGTACTCC GGAGAACATC TCAGCCGCTG TCCCCTCCCA GGGCTCTGTT 
GTGGAGTCAG AACCCGAGCT CGTTGTCAAC CCCTGGGACA TTGTCTTGTG CAGCTCAGGA 
ACCCTCATCT GCTGTGAAAA TGCCGTCGTG GTCCTTATCA TCTTCCACAG CCCCAGCCTG 
CGAGCACCCA TGTTCCTGCT GATAGGCAGC CTGGCTCTTG CAGACCTGCT GGCTGGTCTG 
GGACTCATCA TCAATTTTGT TTTTGCCTAC CTGCTTCAGT CAGAAGCCAC CAAGCTGGTC 
ACAATTGGAC TCATTGTCGC CTCTTTCTCT GCCTCTGTCT GCAGTTTGCT GGCTATCACT 
GTGGACCGCT ACCTCTCGCT GTATTACGCC CTGACGTACC ACTCCGAGAG GACCGTCACC 
TTTACCTATG TCATGCTAGT GATGCTCTGG GGAACTTCCA CCTGCCTGGG GCTGCTGCCT 
GTCATGGGCT GGAACTGCCT GAGGGACGAG TCCACCTGCA GCGTGGTCAG ACCCCTCACT 
AAGAACAACG CCGCCATTCT CTCCATCTCC TTCCTCTTCA TGTTCGCACT GATGCTCCAA 
CTCTACATCC AGATTTGTAA GATTGTGATG AGGCACGCCC ATCAGATAGC CCTGCAGCAC 
CACTTCCTGG CTACGTCGCA CTACGTGACT ACCCGGAAAG GGATCTCCAC CCTGGCTCTC 
ATCCTGGGGA CCTTTGCCGC CTGCTGGATG CCTTTTACCC TCTATTCCTT GATCGCCGAT 
TACACCTACC CCTCCATCTA CACCTACGCC ACCCTCCTGC CCGCCACGTA CAATTCCATC 
ATCAACCCTG TCATATATGC TTTCAGAAAC CAAGAGATCC AGAAAGCCCT CTGCCTCATC 
TGCTGTGGGT GCATCCCTAA CACGCTGTCC CAGAGAGCAC GCTCTCCCAG CGATGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453952.1
CDS	28..1287
Translation	MTAAGGRPRWRAPAPHAAATCGGARSLVGFSRMRAGRGARRAESRARATQLTCRGRHHGRATRVALAIRQHRRSPPSVWACQGVKMNEDPKVNLSGLPRDCIEAGTPENISAAVPSQGSVVESEPELVVNPWDIVLCSSGTLICCENAVVVLIIFHSPSLRAPMFLLIGSLALADLLAGLGLIINFVFAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVMLVMLWGTSTCLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSISFLFMFALMLQLYIQICKIVMRHAHQIALQHHFLATSHYVTTRKGISTLALILGTFAACWMPFTLYSLIADYTYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPNTLSQRARSPSDV

Target ORF information:

RefSeq Version	XM_017598463.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 12 (Gpr12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598463.1

ORF Insert Sequence:

ATGACCGCCG CAGGCGGGCG TCCACGGTGG CGGGCTCCCG CCCCTCACGC AGCTGCGACC 
TGCGGGGGCG CGCGCAGCCT CGTGGGGTTC TCGCGGATGC GCGCCGGGCG GGGAGCGCGC 
AGGGCGGAGA GCCGGGCGCG AGCGACGCAG CTCACCTGCC GCGGGCGCCA CCACGGACGT 
GCCACGCGGG TGGCCCTCGC TATTCGGCAG CACCGAAGGA GCCCCCCCTC GGTCTGGGCG 
TGCCAAGGGG TTAAAATGAA CGAAGACCCG AAGGTCAATT TAAGCGGGCT GCCTCGGGAC 
TGTATAGAAG CTGGTACTCC GGAGAACATC TCAGCCGCTG TCCCCTCCCA GGGCTCTGTT 
GTGGAGTCAG AACCCGAGCT CGTTGTCAAC CCCTGGGACA TTGTCTTGTG CAGCTCAGGA 
ACCCTCATCT GCTGTGAAAA TGCCGTCGTG GTCCTTATCA TCTTCCACAG CCCCAGCCTG 
CGAGCACCCA TGTTCCTGCT GATAGGCAGC CTGGCTCTTG CAGACCTGCT GGCTGGTCTG 
GGACTCATCA TCAATTTTGT TTTTGCCTAC CTGCTTCAGT CAGAAGCCAC CAAGCTGGTC 
ACAATTGGAC TCATTGTCGC CTCTTTCTCT GCCTCTGTCT GCAGTTTGCT GGCTATCACT 
GTGGACCGCT ACCTCTCGCT GTATTACGCC CTGACGTACC ACTCCGAGAG GACCGTCACC 
TTTACCTATG TCATGCTAGT GATGCTCTGG GGAACTTCCA CCTGCCTGGG GCTGCTGCCT 
GTCATGGGCT GGAACTGCCT GAGGGACGAG TCCACCTGCA GCGTGGTCAG ACCCCTCACT 
AAGAACAACG CCGCCATTCT CTCCATCTCC TTCCTCTTCA TGTTCGCACT GATGCTCCAA 
CTCTACATCC AGATTTGTAA GATTGTGATG AGGCACGCCC ATCAGATAGC CCTGCAGCAC 
CACTTCCTGG CTACGTCGCA CTACGTGACT ACCCGGAAAG GGATCTCCAC CCTGGCTCTC 
ATCCTGGGGA CCTTTGCCGC CTGCTGGATG CCTTTTACCC TCTATTCCTT GATCGCCGAT 
TACACCTACC CCTCCATCTA CACCTACGCC ACCCTCCTGC CCGCCACGTA CAATTCCATC 
ATCAACCCTG TCATATATGC TTTCAGAAAC CAAGAGATCC AGAAAGCCCT CTGCCTCATC 
TGCTGTGGGT GCATCCCTAA CACGCTGTCC CAGAGAGCAC GCTCTCCCAG CGATGTGTAG