Grpel1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grpel1
Entrez Gene ID	79563
Full Name	GrpE-like 1, mitochondrial
Synonyms	GrpE#1,GrpE1
General protein information	Preferred Names GrpE-like 1, mitochondrial Names grpE protein homolog 1, mitochondrial mt-GrpE#1 stress-inducible chaperone mt-GrpE#1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	a stress-inducible nucleotide-exchange factor found in the mitochondria [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251142.3	XP_006251204.1	grpE protein homolog 1, mitochondrial isoform X1
NM_024487.4	NP_077813.1	grpE protein homolog 1, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRPEL1	NP_079472.1
Pan troglodytes (chimpanzee)	GRPEL1	XP_526517.2
Saccharomyces cerevisiae (baker's yeast)	MGE1	NP_014875.3
Kluyveromyces lactis	KLLA0D07326g	XP_453388.1
Magnaporthe oryzae (rice blast fungus)	MGG_03214	XP_003716765.1
Oryza sativa (rice)	Os08g0338700	NP_001061586.1
Oryza sativa (rice)	Os09g0284400	NP_001062776.1
Rattus norvegicus (Norway rat)	Grpel1	NP_077813.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011150	XP_309497.4
Neurospora crassa	NCU01516	XP_956965.1
Danio rerio (zebrafish)	grpel1	NP_001032461.1
Schizosaccharomyces pombe (fission yeast)	mge1	NP_596677.1
Mus musculus (house mouse)	Grpel1	NP_077798.1
Gallus gallus (chicken)	GRPEL1	NP_001006458.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012770	XP_307508.3
	GRPEL1	XP_001091412.1
Canis lupus familiaris (dog)	GRPEL1	XP_545902.4
Bos taurus (cattle)	GRPEL1	NP_001029673.1
Drosophila melanogaster (fruit fly)	Roe1	NP_610886.2
Caenorhabditis elegans (roundworm)	C34C12.8	NP_497713.1
Xenopus tropicalis (tropical clawed frog)	grpel1	NP_001016179.1

Gene Ontology
Bibliography

Related articles in PubMed

Unlocking the presequence import pathway.
Schulz C, Schendzielorz A, Rehling P
Trends in cell biology25(5)265-75(2015 May)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Induction of heat shock protein 40 and GrpE mRNAs following transient focal cerebral ischemia in the rat.
Tamura S, Kinouchi H, Izaki K, Okubo A, Sugawara T, Kunizuka H, Mizoi K
Brain research960(1-2)277-81(2003 Jan)

Identification and characterization of a human mitochondrial homologue of the bacterial co-chaperone GrpE.
Choglay AA, Chapple JP, Blatch GL, Cheetham ME
Gene267(1)125-34(2001 Apr)

Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the Co-chaperones of specific Hsp70 members.
Naylor DJ, Stines AP, Hoogenraad NJ, H?j PB
The Journal of biological chemistry273(33)21169-77(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reverse transcription-polymerase chain reaction and in situ hybridization analyses showed significant induction of GrpE mRNA in the ischemic cortex
Title: Induction of heat shock protein 40 and GrpE mRNAs following transient focal cerebral ischemia in the rat.

The following Grpel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grpel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12226	NM_024487.4 Latest version!	Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa36189	XM_006251142.3 Latest version!	Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grpel1 ( NM_024487.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077813.1 Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), mRNA.

Price & Availability↑

CloneID	ORa12226
Clone ID Related Accession (Same CDS sequence)	NM_024487.4
Accession Version	NM_024487.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	grpE protein homolog 1, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U62940.1. On Aug 30, 2012 this sequence version replaced NM_024487.3. ##Evidence-Data-START## Transcript exon combination :: U62940.1, FQ215495.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCTC GGTGCGTGAG GCTGGCGCGG CGCAGCCTCC CGGCTTTGGC GCTGTCGTTC 
AGGCCTTCTC CTCGCTTGTT GTGCACAGCT ACAAAACAAA AGAACAATGG CCAGAACCTG 
GAAGAGGACT TGGGGCATTG TGAGCCAAAG ACAGATCCAT CCTCTGCAGA CAAGACCCTC 
CTGGAAGAGA AGGTGAAGCT GGAAGAGCAG CTGAAGGAGA CCATGGAAAA ATACAAACGT 
GCTTTGGCAG ATACCGAGAA TCTACGGCAG AGAAGCCAGA AGCTGGTAGA AGAGGCCAAG 
TTATATGGCA TCCAGGGTTT CTGCAAGGAC TTGCTGGAGG TTGCAGACAT CCTAGAGAAG 
GCAACCCAGA GTGTTCCAAA GGAGGAGGTC AGCAACAACA ACCCTCACCT GAAGAGTCTT 
TATGAAGGGC TCGTGATGAC TGAAGTCCAG ATTCAGAAGG TGTTCACAAA ACACGGCTTG 
CTCAGGCTTG ACCCCATTGG GGCAAAGTTC GACCCTTATG AACATGAGGC CTTGTTCCAC 
ACCCCTGTGG AGGGGAAAGA ACCAGGCACT GTGGCACTAG TTAGTAAGGT GGGCTACAAG 
CTGCATGGAC GCACCCTGAG GCCAGCTTTG GTGGGGGTGG TGAAGGACGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077813.1
CDS	14..667
Translation	MAARCVRLARRSLPALALSFRPSPRLLCTATKQKNNGQNLEEDLGHCEPKTDPSSADKTLLEEKVKLEEQLKETMEKYKRALADTENLRQRSQKLVEEAKLYGIQGFCKDLLEVADILEKATQSVPKEEVSNNNPHLKSLYEGLVMTEVQIQKVFTKHGLLRLDPIGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKDA

Target ORF information:

RefSeq Version	NM_024487.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024487.4

ORF Insert Sequence:

ATGGCGGCTC GGTGCGTGAG GCTGGCGCGG CGCAGCCTCC CGGCTTTGGC GCTGTCGTTC 
AGGCCTTCTC CTCGCTTGTT GTGCACAGCT ACAAAACAAA AGAACAATGG CCAGAACCTG 
GAAGAGGACT TGGGGCATTG TGAGCCAAAG ACAGATCCAT CCTCTGCAGA CAAGACCCTC 
CTGGAAGAGA AGGTGAAGCT GGAAGAGCAG CTGAAGGAGA CCATGGAAAA ATACAAACGT 
GCTTTGGCAG ATACCGAGAA TCTACGGCAG AGAAGCCAGA AGCTGGTAGA AGAGGCCAAG 
TTATATGGCA TCCAGGGTTT CTGCAAGGAC TTGCTGGAGG TTGCAGACAT CCTAGAGAAG 
GCAACCCAGA GTGTTCCAAA GGAGGAGGTC AGCAACAACA ACCCTCACCT GAAGAGTCTT 
TATGAAGGGC TCGTGATGAC TGAAGTCCAG ATTCAGAAGG TGTTCACAAA ACACGGCTTG 
CTCAGGCTTG ACCCCATTGG GGCAAAGTTC GACCCTTATG AACATGAGGC CTTGTTCCAC 
ACCCCTGTGG AGGGGAAAGA ACCAGGCACT GTGGCACTAG TTAGTAAGGT GGGCTACAAG 
CTGCATGGAC GCACCCTGAG GCCAGCTTTG GTGGGGGTGG TGAAGGACGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grpel1 ( XM_006251142.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251204.1 Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36189
Clone ID Related Accession (Same CDS sequence)	XM_006251142.3
Accession Version	XM_006251142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	grpE protein homolog 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCATA CACTCATACT GACACACCTA AAAAGGCATC CACATTCTTT GGGTGGTGTA 
TGTGATGTGA GCACATTGGG AAGGCCCAAG GTCAACATCA GGCCTTCTCC TCGCTTGTTG 
TGCACAGCTA CAAAACAAAA GAACAATGGC CAGAACCTGG AAGAGGACTT GGGGCATTGT 
GAGCCAAAGA CAGATCCATC CTCTGCAGAC AAGACCCTCC TGGAAGAGAA GGTGAAGCTG 
GAAGAGCAGC TGAAGGAGAC CATGGAAAAA TACAAACGTG CTTTGGCAGA TACCGAGAAT 
CTACGGCAGA GAAGCCAGAA GCTGGTAGAA GAGGCCAAGT TATATGGCAT CCAGGGTTTC 
TGCAAGGACT TGCTGGAGGT TGCAGACATC CTAGAGAAGG CAACCCAGAG TGTTCCAAAG 
GAGGAGGTCA GCAACAACAA CCCTCACCTG AAGAGTCTTT ATGAAGGGCT CGTGATGACT 
GAAGTCCAGA TTCAGAAGGT GTTCACAAAA CACGGCTTGC TCAGGCTTGA CCCCATTGGG 
GCAAAGTTCG ACCCTTATGA ACATGAGGCC TTGTTCCACA CCCCTGTGGA GGGGAAAGAA 
CCAGGCACTG TGGCACTAGT TAGTAAGGTG GGCTACAAGC TGCATGGACG CACCCTGAGG 
CCAGCTTTGG TGGGGGTGGT GAAGGACGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251204.1
CDS	175..867
Translation	MWHTLILTHLKRHPHSLGGVCDVSTLGRPKVNIRPSPRLLCTATKQKNNGQNLEEDLGHCEPKTDPSSADKTLLEEKVKLEEQLKETMEKYKRALADTENLRQRSQKLVEEAKLYGIQGFCKDLLEVADILEKATQSVPKEEVSNNNPHLKSLYEGLVMTEVQIQKVFTKHGLLRLDPIGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKDA

Target ORF information:

RefSeq Version	XM_006251142.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GrpE-like 1, mitochondrial (Grpel1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251142.3

ORF Insert Sequence:

ATGTGGCATA CACTCATACT GACACACCTA AAAAGGCATC CACATTCTTT GGGTGGTGTA 
TGTGATGTGA GCACATTGGG AAGGCCCAAG GTCAACATCA GGCCTTCTCC TCGCTTGTTG 
TGCACAGCTA CAAAACAAAA GAACAATGGC CAGAACCTGG AAGAGGACTT GGGGCATTGT 
GAGCCAAAGA CAGATCCATC CTCTGCAGAC AAGACCCTCC TGGAAGAGAA GGTGAAGCTG 
GAAGAGCAGC TGAAGGAGAC CATGGAAAAA TACAAACGTG CTTTGGCAGA TACCGAGAAT 
CTACGGCAGA GAAGCCAGAA GCTGGTAGAA GAGGCCAAGT TATATGGCAT CCAGGGTTTC 
TGCAAGGACT TGCTGGAGGT TGCAGACATC CTAGAGAAGG CAACCCAGAG TGTTCCAAAG 
GAGGAGGTCA GCAACAACAA CCCTCACCTG AAGAGTCTTT ATGAAGGGCT CGTGATGACT 
GAAGTCCAGA TTCAGAAGGT GTTCACAAAA CACGGCTTGC TCAGGCTTGA CCCCATTGGG 
GCAAAGTTCG ACCCTTATGA ACATGAGGCC TTGTTCCACA CCCCTGTGGA GGGGAAAGAA 
CCAGGCACTG TGGCACTAGT TAGTAAGGTG GGCTACAAGC TGCATGGACG CACCCTGAGG 
CCAGCTTTGG TGGGGGTGGT GAAGGACGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unlocking the presequence import pathway.
Trends in cell biology25(5)265-75(2015 May)
Schulz C,Schendzielorz A,Rehling P

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Induction of heat shock protein 40 and GrpE mRNAs following transient focal cerebral ischemia in the rat.
Brain research960(1-2)277-81(2003 Jan)
Tamura S,Kinouchi H,Izaki K,Okubo A,Sugawara T,Kunizuka H,Mizoi K

Identification and characterization of a human mitochondrial homologue of the bacterial co-chaperone GrpE.
Gene267(1)125-34(2001 Apr)
Choglay AA,Chapple JP,Blatch GL,Cheetham ME

Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the Co-chaperones of specific Hsp70 members.
The Journal of biological chemistry273(33)21169-77(1998 Aug)
Naylor DJ,Stines AP,Hoogenraad NJ,H?j PB