Timm22 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timm22
Entrez Gene ID	79463
Full Name	translocase of inner mitochondrial membrane 22
General protein information	Preferred Names translocase of inner mitochondrial membrane 22 Names mitochondrial import inner membrane translocase subunit Tim22 translocase of inner mitochondrial membrane 22 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	controls the import of integral inner membrane proteins without a matrix targeting signal into the mitochondria [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032618.1	NP_116007.1	mitochondrial import inner membrane translocase subunit Tim22

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Timm22	NP_116007.1
Gallus gallus (chicken)	TIMM22	XP_415839.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003199	XP_312898.3
Xenopus tropicalis (tropical clawed frog)	timm22	NP_001011397.1
Homo sapiens (human)	TIMM22	NP_037469.2
Canis lupus familiaris (dog)	TIMM22	XP_537758.1
Mus musculus (house mouse)	Timm22	NP_062792.2
Bos taurus (cattle)	TIMM22	NP_001029670.1
Drosophila melanogaster (fruit fly)	CG31229	NP_732364.1
Caenorhabditis elegans (roundworm)	CELE_C47G2.3	NP_496412.1
Pan troglodytes (chimpanzee)	TIMM22	XP_001153337.1
Danio rerio (zebrafish)	LOC100537779	XP_003198763.1
Schizosaccharomyces pombe (fission yeast)	tim22	NP_596362.1

Gene Ontology
Bibliography

Related articles in PubMed

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.
Kang Y, Stroud DA, Baker MJ, De Souza DP, Frazier AE, Liem M, Tull D, Mathivanan S, McConville MJ, Thorburn DR, Ryan MT, Stojanovski D
Molecular cell67(3)457-470.e5(2017 Aug)

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Vukotic M, Nolte H, K?nig T, Saita S, Ananjew M, Kr?ger M, Tatsuta T, Langer T
Molecular cell67(3)471-483.e7(2017 Aug)

TIM29 is a subunit of the human carrier translocase required for protein transport.
Callegari S, Richter F, Chojnacka K, Jans DC, Lorenzi I, Pacheu-Grau D, Jakobs S, Lenz C, Urlaub H, Dudek J, Chacinska A, Rehling P
FEBS letters590(23)4147-4158(2016 Dec)

Translocation of proteins into mitochondria.
Neupert W, Herrmann JM
Annual review of biochemistry76723-49(2007)

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
M?hlenbein N, Hofmann S, Rothbauer U, Bauer MF
The Journal of biological chemistry279(14)13540-6(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timm22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timm22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12265	NM_032618.1 Latest version!	Rattus norvegicus translocase of inner mitochondrial membrane 22 (Timm22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timm22 ( NM_032618.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_116007.1 Rattus norvegicus translocase of inner mitochondrial membrane 22 (Timm22), mRNA.

Price & Availability↑

CloneID	ORa12265
Clone ID Related Accession (Same CDS sequence)	NM_032618.1
Accession Version	NM_032618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial import inner membrane translocase subunit Tim22
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM107575.1 and AABR07072152.1. On or before Feb 8, 2013 this sequence version replaced XM_001080619.3, XM_340856.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FM107575.1, AF223951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCAAGGCTGG GGCTTCAGCT CCTGAGGCGG CGGGTTCCGC CGAAGCTCCG 
TTGCAGTACA GCCTTCTTCT TCAGCACCTG GTGGGCGACA AGCGTCAGCC ACGGCTTCTG 
GAGCCTGGCA GTCTGGGCGG GATCCCGAGT CCTGCCAAGA GCGAGGAGCA GAAGATGATC 
GAGAGGGCGA TGGAAAGTTG CGCCTTCAAG GCTGTGCTGG CCTGTGTGGG AGGATTTGTC 
TTGGGAGGCG CATTTGGTGT CTTTACTGCT GGCATTGATA CCAACGTGGG CTTTGACCCC 
AAGGACCCTT ACCGGACACC AACTGCAAGA GAAGTCCTGA AGGACATGGG ACAGAGAGGA 
ATGTCCTATG CCAAAAATTT TGCCATTGTG GGTGCCATGT TTTCCTGTAC TGAGTGTCTG 
GTAGAGTCTT ACCGGGGGAA GTCAGACTGG AAGAACAGCG TCATCAGCGG CTGCATCACT 
GGTGGAGCCA TCGGCTTCAG AGCTGGAGTA AAGGCCGGGG CCATAGGTTG TGGAGGTTTT 
GCTGCTTTCT CTGCTGCAAT CGATTATTAC CTACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116007.1
CDS	13..591
Translation	MAAAKAGASAPEAAGSAEAPLQYSLLLQHLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIERAMESCAFKAVLACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAREVLKDMGQRGMSYAKNFAIVGAMFSCTECLVESYRGKSDWKNSVISGCITGGAIGFRAGVKAGAIGCGGFAAFSAAIDYYLR

Target ORF information:

RefSeq Version	NM_032618.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus translocase of inner mitochondrial membrane 22 (Timm22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032618.1

ORF Insert Sequence:

ATGGCGGCCG CCAAGGCTGG GGCTTCAGCT CCTGAGGCGG CGGGTTCCGC CGAAGCTCCG 
TTGCAGTACA GCCTTCTTCT TCAGCACCTG GTGGGCGACA AGCGTCAGCC ACGGCTTCTG 
GAGCCTGGCA GTCTGGGCGG GATCCCGAGT CCTGCCAAGA GCGAGGAGCA GAAGATGATC 
GAGAGGGCGA TGGAAAGTTG CGCCTTCAAG GCTGTGCTGG CCTGTGTGGG AGGATTTGTC 
TTGGGAGGCG CATTTGGTGT CTTTACTGCT GGCATTGATA CCAACGTGGG CTTTGACCCC 
AAGGACCCTT ACCGGACACC AACTGCAAGA GAAGTCCTGA AGGACATGGG ACAGAGAGGA 
ATGTCCTATG CCAAAAATTT TGCCATTGTG GGTGCCATGT TTTCCTGTAC TGAGTGTCTG 
GTAGAGTCTT ACCGGGGGAA GTCAGACTGG AAGAACAGCG TCATCAGCGG CTGCATCACT 
GGTGGAGCCA TCGGCTTCAG AGCTGGAGTA AAGGCCGGGG CCATAGGTTG TGGAGGTTTT 
GCTGCTTTCT CTGCTGCAAT CGATTATTAC CTACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.
Molecular cell67(3)457-470.e5(2017 Aug)
Kang Y,Stroud DA,Baker MJ,De Souza DP,Frazier AE,Liem M,Tull D,Mathivanan S,McConville MJ,Thorburn DR,Ryan MT,Stojanovski D

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Molecular cell67(3)471-483.e7(2017 Aug)
Vukotic M,Nolte H,K?nig T,Saita S,Ananjew M,Kr?ger M,Tatsuta T,Langer T

TIM29 is a subunit of the human carrier translocase required for protein transport.
FEBS letters590(23)4147-4158(2016 Dec)
Callegari S,Richter F,Chojnacka K,Jans DC,Lorenzi I,Pacheu-Grau D,Jakobs S,Lenz C,Urlaub H,Dudek J,Chacinska A,Rehling P

Translocation of proteins into mitochondria.
Annual review of biochemistry76723-49(2007)
Neupert W,Herrmann JM

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
The Journal of biological chemistry279(14)13540-6(2004 Apr)
M?hlenbein N,Hofmann S,Rothbauer U,Bauer MF