Arf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arf4
Entrez Gene ID	79120
Full Name	ADP-ribosylation factor 4
General protein information	Preferred Names ADP-ribosylation factor 4 Names ADP-ribosylation factor 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	human homolog binds EGFR and mediates the EGF-dependent activation of phospholipase D2 (PLD2) and of transcription factor AP-1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024151.1	NP_077065.1	ADP-ribosylation factor 4

Pathways from BioSystems

Homologies

	ARF5	XP_001103974.1
Homo sapiens (human)	ARF4	NP_001651.1
Pan troglodytes (chimpanzee)	ARF4	XP_003950181.1
Gallus gallus (chicken)	ARF4	XP_001232784.3
Canis lupus familiaris (dog)	ARF4	XP_849047.1
Drosophila melanogaster (fruit fly)	Arf102F	NP_524631.1
Xenopus tropicalis (tropical clawed frog)	arf4	NP_001025648.1
Mus musculus (house mouse)	Arf4	NP_031505.1
Danio rerio (zebrafish)	arf4a	NP_001003590.1
Rattus norvegicus (Norway rat)	Arf4	NP_077065.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002931	XP_003436315.1

Gene Ontology
Bibliography

Related articles in PubMed

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Yu J, Chia J, Canning CA, Jones CM, Bard FA, Virshup DM
Developmental cell29(3)277-91(2014 May)

Arf4 is required for Mammalian development but dispensable for ciliary assembly.
Follit JA, San Agustin JT, Jonassen JA, Huang T, Rivera-Perez JA, Tremblay KD, Pazour GJ
PLoS genetics10(2)e1004170(2014 Feb)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Arf4 determines dentate gyrus-mediated pattern separation by regulating dendritic spine development.
Jain S, Yoon SY, Zhu L, Brodbeck J, Dai J, Walker D, Huang Y
PloS one7(9)e46340(2012)

Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration.
Jang SY, Jang SW, Ko J
Cancer letters314(2)185-97(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a functional role for CAPS1 in regulating DCV trafficking in the trans-Golgi network; this activity occurs via protein-protein interaction with ARF4/ARF5 in a GDP-dependent manner.
Title: Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.

The following Arf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06855	NM_024151.1 Latest version!	Rattus norvegicus ADP-ribosylation factor 4 (Arf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arf4 ( NM_024151.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077065.1 Rattus norvegicus ADP-ribosylation factor 4 (Arf4), mRNA.

Price & Availability↑

CloneID	ORa06855
Clone ID Related Accession (Same CDS sequence)	NM_024151.1
Accession Version	NM_024151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L12383.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L12383.1, FQ234248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCTCA CCATCTCCTC TCTCTTCTCG CGCCTCTTCG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCGGGCAAG ACGACGATTC TGTATAAACT GAAACTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTTTGGGA TGTTGGTGGT CAAGATAAAA TTAGGCCTCT CTGGAGGCAT 
TACTTCCAGA ATACCCAGGG TCTCATTTTC GTGGTAGATA GCAATGATCG TGAAAGAATT 
CAGGAAGGAG CAGCCGTGCT GCAGAAAATG CTTCTGGAAG ATGAGCTGCA GGATGCTGTG 
CTGCTGCTTT TTGCCAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAGATGACA 
GACAAGTTAG GTCTTCAGTC TCTCCGGAAC AGAACATGGT ATGTCCAAGC CACTTGTGCT 
ACACAAGGAA CTGGTCTGTA CGAGGGGCTG GATTGGCTGT CAAATGAACT TTCAAAACGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077065.1
CDS	220..762
Translation	MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAAVLQKMLLEDELQDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR

Target ORF information:

RefSeq Version	NM_024151.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor 4 (Arf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024151.1

ORF Insert Sequence:

ATGGGCCTCA CCATCTCCTC TCTCTTCTCG CGCCTCTTCG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCGGGCAAG ACGACGATTC TGTATAAACT GAAACTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTTTGGGA TGTTGGTGGT CAAGATAAAA TTAGGCCTCT CTGGAGGCAT 
TACTTCCAGA ATACCCAGGG TCTCATTTTC GTGGTAGATA GCAATGATCG TGAAAGAATT 
CAGGAAGGAG CAGCCGTGCT GCAGAAAATG CTTCTGGAAG ATGAGCTGCA GGATGCTGTG 
CTGCTGCTTT TTGCCAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAGATGACA 
GACAAGTTAG GTCTTCAGTC TCTCCGGAAC AGAACATGGT ATGTCCAAGC CACTTGTGCT 
ACACAAGGAA CTGGTCTGTA CGAGGGGCTG GATTGGCTGT CAAATGAACT TTCAAAACGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Developmental cell29(3)277-91(2014 May)
Yu J,Chia J,Canning CA,Jones CM,Bard FA,Virshup DM

Arf4 is required for Mammalian development but dispensable for ciliary assembly.
PLoS genetics10(2)e1004170(2014 Feb)
Follit JA,San Agustin JT,Jonassen JA,Huang T,Rivera-Perez JA,Tremblay KD,Pazour GJ

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Arf4 determines dentate gyrus-mediated pattern separation by regulating dendritic spine development.
PloS one7(9)e46340(2012)
Jain S,Yoon SY,Zhu L,Brodbeck J,Dai J,Walker D,Huang Y

Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration.
Cancer letters314(2)185-97(2012 Jan)
Jang SY,Jang SW,Ko J