Mthfsd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfsd
Entrez Gene ID	687539
Full Name	methenyltetrahydrofolate synthetase domain containing
General protein information	Preferred Names methenyltetrahydrofolate synthetase domain containing Names LOW QUALITY PROTEIN: methenyltetrahydrofolate synthase domain-containing protein methenyltetrahydrofolate synthase domain-containing protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_008774251.1	XP_008772473.1	LOW QUALITY PROTEIN: methenyltetrahydrofolate synthase domain-containing protein isoform X2
XM_017601448.1	XP_017456937.1	methenyltetrahydrofolate synthase domain-containing protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTHFSD	NP_073601.2
Pan troglodytes (chimpanzee)	MTHFSD	XP_511153.2
	MTHFSD	XP_001085469.2
Danio rerio (zebrafish)	mthfsd	NP_001038564.1
Drosophila melanogaster (fruit fly)	lost	NP_649466.1
Mus musculus (house mouse)	Mthfsd	NP_001159954.1
Gallus gallus (chicken)	MTHFSD	XP_004944333.1
Oryza sativa (rice)	Os12g0168000	NP_001066251.1
Bos taurus (cattle)	MTHFSD	NP_001039507.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000622	XP_310457.5
Xenopus tropicalis (tropical clawed frog)	mthfsd	NP_001106431.1
Canis lupus familiaris (dog)	MTHFSD	XP_005620649.1
Rattus norvegicus (Norway rat)	Mthfsd	XP_006255787.1
Arabidopsis thaliana (thale cress)	AT1G76730	NP_565139.1

Gene Ontology

The following Mthfsd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfsd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa50228	XM_008774251.1 Latest version!	Rattus norvegicus methenyltetrahydrofolate synthetase domain containing (Mthfsd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfsd ( XM_008774251.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008772473.1 Rattus norvegicus methenyltetrahydrofolate synthetase domain containing (Mthfsd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa50228
Clone ID Related Accession (Same CDS sequence)	XM_008774251.1
Accession Version	XM_008774251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: methenyltetrahydrofolate synthase domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGCGTCTGA CGTCATCGGT GCGCCGACAA GCTCTACGGG AGGCGTCGCT CGGTGACGTG 
TTGGTCATGG AGGCCCGGGC GGGAGATGTC TCCAAGCAAA GCATACGTGA ACGGATTTGG 
GACTACATGG AATCCCATAA TATAGCTGAT TTTCCCCGAC CTGTTCATCA CAGGATCCCC 
AACTTCAAGG GGTCCTACCT GGCTGGCCAA AGCATCAGAG ACCTGGAAGT CTTTGCTGGG 
ACACAGGAAG TGAAAGTGGA CCCTGATAAG CCCCTGGAAG GTGTCCGCTT ACTGGCACTG 
CAGAGCAGAA AAACACTGCT GGTCCCAACA CCTCGACTGA GGACAGGGCT GTTTAACAAG 
ATCATACCAC CCCCTGGAGC CACCAAAGAC ATACTGAGGA AATGTGCCAC CTCCCAGGGT 
GTGCGGAACT TCAGCGTCCC GGTGGGCTTG GATTCCAATG TCCTCGTGGA TTTAGTTGTG 
GTGGGATCTG TTGCTGTGTC TGAGAAAGGC TGGCGAATTG GGAAGGGAGA AGGCTACGCT 
GATCTTGAAT ATGCCATGAT GGTGTCGATG GGAGCTGTCC ACGAGGGGAC ATTGGTCGTC 
ACGATTGTCC ACGACTGCCA GGTCGTTGAC ATCCCTGAGG CTCTCCTGGA GGACCACGAC 
CTCACTGTAG ACTACATCCT CACTCCCACC AGGGTCATCG CCACAGGCTG TGCACGCCCA 
AAGCCAACAG GGATCCTGTG GTCCAAGGTC AGCTGTGAGA TGCTTACGAA AATCCCAGTG 
CTCAGGAGCC TCCGTGAGCG AGAGAAGCGG GCGGGGACCG ATGTCACTCT TCAGGCTGAG 
CCTGGCAACA CACAGCAGCC AGCTCCCAGC ACTGTCAGGA GGCCCCAGGA TAGACCACAG 
TCACAACCAG CTCCCAGCAC TGTCAGGAGA CCCCAGGATA GACCACAGTC ACAAGCAGGT 
TTCCGAGGAA GGTCCCATAG CTCCCTGGAG GGGACGGACA CCCACCTAGC TGCCACTGTG 
TGCTTGGGAA ACCTTCCCTA CAGTGCTCGT GTGCGTGAGC TGAAGCGAGC CCCCTGACTC 
GAACGGATAG CACCATAGAA GNNGCAGGGG CCACAGCACA GAGCAATTCT ACACTATACA 
GACTCAGCTG CTGCCCAGCA GGCTGCCTCT CTCCTACAGG GCCTGCGACT GGGTGACAAT 
GCTTTGAGGG TGTCACTGGG GCAGCAGAGG GATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008772473.1
CDS	5..1243
Translation	MRLTSSVRRQALREASLGDVLVMEARAGDVSKQSIRERIWDYMESHNIADFPRPVHHRIPNFKGSYLAGQSIRDLEVFAGTQEVKVDPDKPLEGVRLLALQSRKTLLVPTPRLRTGLFNKIIPPPGATKDILRKCATSQGVRNFSVPVGLDSNVLVDLVVVGSVAVSEKGWRIGKGEGYADLEYAMMVSMGAVHEGTLVVTIVHDCQVVDIPEALLEDHDLTVDYILTPTRVIATGCARPKPTGILWSKVSCEMLTKIPVLRSLREREKRAGTDVTLQAEPGNTQQPAPSTVRRPQDRPQSQPAPSTVRRPQDRPQSQAGFRGRSHSSLEGTDTHLAATVCLGNLPYSARVRELKRAPLERIAPKnullQGPQHRAILHYTDSAAAQQAASLLQGLRLGDNALRVSLGQQRDT

Target ORF information:

RefSeq Version	XM_008774251.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methenyltetrahydrofolate synthetase domain containing (Mthfsd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008774251.1

ORF Insert Sequence:

ATGCGTCTGA CGTCATCGGT GCGCCGACAA GCTCTACGGG AGGCGTCGCT CGGTGACGTG 
TTGGTCATGG AGGCCCGGGC GGGAGATGTC TCCAAGCAAA GCATACGTGA ACGGATTTGG 
GACTACATGG AATCCCATAA TATAGCTGAT TTTCCCCGAC CTGTTCATCA CAGGATCCCC 
AACTTCAAGG GGTCCTACCT GGCTGGCCAA AGCATCAGAG ACCTGGAAGT CTTTGCTGGG 
ACACAGGAAG TGAAAGTGGA CCCTGATAAG CCCCTGGAAG GTGTCCGCTT ACTGGCACTG 
CAGAGCAGAA AAACACTGCT GGTCCCAACA CCTCGACTGA GGACAGGGCT GTTTAACAAG 
ATCATACCAC CCCCTGGAGC CACCAAAGAC ATACTGAGGA AATGTGCCAC CTCCCAGGGT 
GTGCGGAACT TCAGCGTCCC GGTGGGCTTG GATTCCAATG TCCTCGTGGA TTTAGTTGTG 
GTGGGATCTG TTGCTGTGTC TGAGAAAGGC TGGCGAATTG GGAAGGGAGA AGGCTACGCT 
GATCTTGAAT ATGCCATGAT GGTGTCGATG GGAGCTGTCC ACGAGGGGAC ATTGGTCGTC 
ACGATTGTCC ACGACTGCCA GGTCGTTGAC ATCCCTGAGG CTCTCCTGGA GGACCACGAC 
CTCACTGTAG ACTACATCCT CACTCCCACC AGGGTCATCG CCACAGGCTG TGCACGCCCA 
AAGCCAACAG GGATCCTGTG GTCCAAGGTC AGCTGTGAGA TGCTTACGAA AATCCCAGTG 
CTCAGGAGCC TCCGTGAGCG AGAGAAGCGG GCGGGGACCG ATGTCACTCT TCAGGCTGAG 
CCTGGCAACA CACAGCAGCC AGCTCCCAGC ACTGTCAGGA GGCCCCAGGA TAGACCACAG 
TCACAACCAG CTCCCAGCAC TGTCAGGAGA CCCCAGGATA GACCACAGTC ACAAGCAGGT 
TTCCGAGGAA GGTCCCATAG CTCCCTGGAG GGGACGGACA CCCACCTAGC TGCCACTGTG 
TGCTTGGGAA ACCTTCCCTA CAGTGCTCGT GTGCGTGAGC TGAAGCGAGC CCCCTGACTC 
GAACGGATAG CACCATAGAA GNNGCAGGGG CCACAGCACA GAGCAATTCT ACACTATACA 
GACTCAGCTG CTGCCCAGCA GGCTGCCTCT CTCCTACAGG GCCTGCGACT GGGTGACAAT 
GCTTTGAGGG TGTCACTGGG GCAGCAGAGG GATACGTGA