Acot4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acot4
Entrez Gene ID	681337
Full Name	acyl-CoA thioesterase 4
General protein information	Preferred Names acyl-CoA thioesterase 4 Names acyl-coenzyme A thioesterase 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109440.1	NP_001102910.1	acyl-coenzyme A thioesterase 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC512541	XP_002691046.1
Pan troglodytes (chimpanzee)	ACOT4	XP_510049.2
Mus musculus (house mouse)	Acot4	NP_599008.3
	ACOT4	XP_001091182.1
Homo sapiens (human)	ACOT4	NP_689544.3
Canis lupus familiaris (dog)	ACOT4	XP_547893.2
Bos taurus (cattle)	ACOT4	NP_001092411.2
Rattus norvegicus (Norway rat)	Acot4	NP_001102910.1

Gene Ontology
Bibliography

Related articles in PubMed

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs.
Hunt MC, Rautanen A, Westin MA, Svensson LT, Alexson SE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(11)1855-64(2006 Sep)

The identification of a succinyl-CoA thioesterase suggests a novel pathway for succinate production in peroxisomes.
Westin MA, Hunt MC, Alexson SE
The Journal of biological chemistry280(46)38125-32(2005 Nov)

Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.
Kikuchi M, Hatano N, Yokota S, Shimozawa N, Imanaka T, Taniguchi H
The Journal of biological chemistry279(1)421-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Acot4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acot4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09944	NM_001109440.1 Latest version!	Rattus norvegicus acyl-CoA thioesterase 4 (Acot4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acot4 ( NM_001109440.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102910.1 Rattus norvegicus acyl-CoA thioesterase 4 (Acot4), mRNA.

Price & Availability↑

CloneID	ORa09944
Clone ID Related Accession (Same CDS sequence)	NM_001109440.1
Accession Version	NM_001109440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	acyl-coenzyme A thioesterase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473982.1. On or before Oct 4, 2007 this sequence version replaced XM_001059880.1, XM_001059814.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGA CGCTGAGCCT GGAGCCCGCC GGCCGCAGCT GCTGGGACGA GCCGCTGAGC 
ATCACGGTGC GCGGCCTGGC CCCGGAGCAG CCCGTCACGC TGCGCGCCGC CCTGCGCGAC 
GAGAAGGGCG CGCTCTTCCG AGCCCACGCG CGCTACCACG CCGACTCCCA CGGAATGCTG 
GACCTGGCGC GCGCTCCCGC GCTGGGCGGC AGCTTCGCGG GGCTCGAGCC CATGGGGCTG 
CTCTGGGCTA TGGAACCCGA ACGGCCTTTC TGGCGTCTGA TCAAGCGCGA CGTGCAGACG 
CCCTTCGTGG TGGAGCTGGA GGTGCTGGAC GGACACGAGC CCGACGGCGG ACAGCGGCTT 
GCACGGGCGG TGCACGAGCG TCACTTCATG GCTCCCGGGG TGCGGCGCGT GCCCGTGCGC 
GAGGGCCGGG TGCGCGCCAC GCTCTTTCTG CCTCCAGGAC AAGGACCCTT TCCAGGGATC 
ATCGATGTCT ATGGTGTTGG AGGGGGCCTA TTGGAATACC GAGCCAGCCT TCTGGCTGGT 
CATGGCTTTG CCACATTGGC TCTGGCTTTT TATGGCTTTG AAGATCTCCC TAAAGAGTTC 
AATGTCATAG AAGTGGACTA CTTTGAAGAA GCAGTGTGCT ACATGCTCCA ACACCCGAAG 
GTAAAAGGCC CAGACATTGG ACTTCTGGGT CTTTCTCTAG GAGCTGATGT TTGCCTGATC 
ATGGCTTCCT TCTTGAAGAA TGTTTCAGCC ACAGTTTCCA TCAATGGCTC TGCATTCAGC 
GGAAACAGAT ACATACACTA CAAGCAGACT ATGATTCCAC CATTGGGCCA TGACCTGAGG 
AGAACGAAGG TGGCTTTCTC AGGCATTCTG GACATTGTGG ATATACGGAA TGACGCTGTA 
GGAGGCTGTG AGAACCCCAG CATGATACCA ATAGAGAAGG CCAAGGGGCC CATCCTCTTT 
GTTGCTGGTC AGGATGACCA TTGCTGGAGG AGTGAGTTAT ACACTCAGAT AGCCTCGGAA 
CGGTTACAGG CTCACGGAAA GGAGAGGCCT CAGATAATCT CTTACCCTGG GACTGGGCAT 
TACATCGAGC CACCTTACTT CCCCATGTGC CCAGCTTCCC TGCACAAAAT AGTGAACAAA 
GCTGTGGTCT GGGGAGGGGA GGTCAGGGCT CACTCAAAAG CCCAGATAGA TGCATGGAAG 
CAAATTCTAT CCTTCTTTGG CAAACACCTG GACAGTACTC ACAACAGAGC CTCCTGCAGA 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102910.1
CDS	134..1399
Translation	MAATLSLEPAGRSCWDEPLSITVRGLAPEQPVTLRAALRDEKGALFRAHARYHADSHGMLDLARAPALGGSFAGLEPMGLLWAMEPERPFWRLIKRDVQTPFVVELEVLDGHEPDGGQRLARAVHERHFMAPGVRRVPVREGRVRATLFLPPGQGPFPGIIDVYGVGGGLLEYRASLLAGHGFATLALAFYGFEDLPKEFNVIEVDYFEEAVCYMLQHPKVKGPDIGLLGLSLGADVCLIMASFLKNVSATVSINGSAFSGNRYIHYKQTMIPPLGHDLRRTKVAFSGILDIVDIRNDAVGGCENPSMIPIEKAKGPILFVAGQDDHCWRSELYTQIASERLQAHGKERPQIISYPGTGHYIEPPYFPMCPASLHKIVNKAVVWGGEVRAHSKAQIDAWKQILSFFGKHLDSTHNRASCRL

Target ORF information:

RefSeq Version	NM_001109440.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA thioesterase 4 (Acot4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109440.1

ORF Insert Sequence:

ATGGCGGCGA CGCTGAGCCT GGAGCCCGCC GGCCGCAGCT GCTGGGACGA GCCGCTGAGC 
ATCACGGTGC GCGGCCTGGC CCCGGAGCAG CCCGTCACGC TGCGCGCCGC CCTGCGCGAC 
GAGAAGGGCG CGCTCTTCCG AGCCCACGCG CGCTACCACG CCGACTCCCA CGGAATGCTG 
GACCTGGCGC GCGCTCCCGC GCTGGGCGGC AGCTTCGCGG GGCTCGAGCC CATGGGGCTG 
CTCTGGGCTA TGGAACCCGA ACGGCCTTTC TGGCGTCTGA TCAAGCGCGA CGTGCAGACG 
CCCTTCGTGG TGGAGCTGGA GGTGCTGGAC GGACACGAGC CCGACGGCGG ACAGCGGCTT 
GCACGGGCGG TGCACGAGCG TCACTTCATG GCTCCCGGGG TGCGGCGCGT GCCCGTGCGC 
GAGGGCCGGG TGCGCGCCAC GCTCTTTCTG CCTCCAGGAC AAGGACCCTT TCCAGGGATC 
ATCGATGTCT ATGGTGTTGG AGGGGGCCTA TTGGAATACC GAGCCAGCCT TCTGGCTGGT 
CATGGCTTTG CCACATTGGC TCTGGCTTTT TATGGCTTTG AAGATCTCCC TAAAGAGTTC 
AATGTCATAG AAGTGGACTA CTTTGAAGAA GCAGTGTGCT ACATGCTCCA ACACCCGAAG 
GTAAAAGGCC CAGACATTGG ACTTCTGGGT CTTTCTCTAG GAGCTGATGT TTGCCTGATC 
ATGGCTTCCT TCTTGAAGAA TGTTTCAGCC ACAGTTTCCA TCAATGGCTC TGCATTCAGC 
GGAAACAGAT ACATACACTA CAAGCAGACT ATGATTCCAC CATTGGGCCA TGACCTGAGG 
AGAACGAAGG TGGCTTTCTC AGGCATTCTG GACATTGTGG ATATACGGAA TGACGCTGTA 
GGAGGCTGTG AGAACCCCAG CATGATACCA ATAGAGAAGG CCAAGGGGCC CATCCTCTTT 
GTTGCTGGTC AGGATGACCA TTGCTGGAGG AGTGAGTTAT ACACTCAGAT AGCCTCGGAA 
CGGTTACAGG CTCACGGAAA GGAGAGGCCT CAGATAATCT CTTACCCTGG GACTGGGCAT 
TACATCGAGC CACCTTACTT CCCCATGTGC CCAGCTTCCC TGCACAAAAT AGTGAACAAA 
GCTGTGGTCT GGGGAGGGGA GGTCAGGGCT CACTCAAAAG CCCAGATAGA TGCATGGAAG 
CAAATTCTAT CCTTCTTTGG CAAACACCTG GACAGTACTC ACAACAGAGC CTCCTGCAGA 
TTGTAG