Atg4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg4a
Entrez Gene ID	678769
Full Name	autophagy related 4A, cysteine peptidase
Synonyms	Agt4a,Atg4al1,RGD1588268
General protein information	Preferred Names autophagy related 4A, cysteine peptidase Names cysteine protease ATG4A autophagy related 4 homolog A autophagy related 4A, cysteine peptidase-like 1 cysteine protease ATG4A-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq33

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126298.1	NP_001119770.1	cysteine protease ATG4A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ATG4A	NP_001258915.1
Danio rerio (zebrafish)	atg4a	NP_001019605.1
Drosophila melanogaster (fruit fly)	Atg4	NP_608563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008497	XP_316946.4
Caenorhabditis elegans (roundworm)	atg-4.1	NP_493375.2
Homo sapiens (human)	ATG4A	NP_443168.2
Pan troglodytes (chimpanzee)	ATG4A	NP_001233457.1
	ATG4A	XP_001097251.1
Oryza sativa (rice)	Os04g0682000	NP_001054293.1
Bos taurus (cattle)	ATG4A	NP_001001171.1
Rattus norvegicus (Norway rat)	Atg4al1	NP_001119770.1
Saccharomyces cerevisiae (baker's yeast)	ATG4	NP_014176.2
Kluyveromyces lactis	KLLA0D19536g	XP_453929.1
Eremothecium gossypii	AGOS_ABL191W	NP_982756.1
Arabidopsis thaliana (thale cress)	AT2G44140	NP_850412.1
Arabidopsis thaliana (thale cress)	AT3G59950	NP_191554.1
Xenopus tropicalis (tropical clawed frog)	atg4a	NP_001016619.1
Canis lupus familiaris (dog)	ATG4A	XP_538136.3
Mus musculus (house mouse)	Atg4a	NP_777364.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene and alternative splicing annotation with AIR.
Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G
Genome research15(1)54-66(2005 Jan)

LC3 conjugation system in mammalian autophagy.
Tanida I, Ueno T, Kominami E
The international journal of biochemistry & cell biology36(12)2503-18(2004 Dec)

LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.
Kabeya Y, Mizushima N, Yamamoto A, Oshitani-Okamoto S, Ohsumi Y, Yoshimori T
Journal of cell science117(Pt 13)2805-12(2004 Jun)

The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A.
Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z
The Journal of biological chemistry278(16)14053-8(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01405	NM_001126298.1 Latest version!	Rattus norvegicus autophagy related 4A, cysteine peptidase (Atg4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg4a ( NM_001126298.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001119770.1 Rattus norvegicus autophagy related 4A, cysteine peptidase (Atg4a), mRNA.

Price & Availability↑

CloneID	ORa01405
Clone ID Related Accession (Same CDS sequence)	NM_001126298.1
Accession Version	NM_001126298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	cysteine protease ATG4A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC160890.1. On May 7, 2008 this sequence version replaced XM_001053095.1. ##Evidence-Data-START## Transcript exon combination :: BC160890.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCAG TTATGTCCAA GTATGAAAAC CAGATTATTA TTTTCACTGA ATACCTAGAA 
GAATTCCCAG ATACAGATGA GCTAGTATGG ATCCTGGGGA AGCCACATCT CCTTAAGACA 
GAAAAATCTA AGCTGTTGTC TGATATAAGT GCTCGTCTAT GGTTTACATA CAGAAGGAAA 
TTTTCACCGA TTGGGGGAAC AGGCCCTTCG TCGGATGCTG GCTGGGGATG TATGCTGCGC 
TGTGGGCAGA TGATGCTGGC TCAAGCCCTC ATCTGTAGAC ACTTGGGAAG GGATTGGAAC 
TGGGAGAGAC AAAAAGAACA ACCCAAAGAA TACCAAAGGA TACTGCAGTG TTTCCTAGAT 
AGAAAAGACT GTTGCTATTC AATCCATCAG ATGGCACAAA TGGGTGTAGG AGAAGGGAAA 
TCAATTGGCG AATGGTTTGG ACCAAATACA GTTGCACAGG TGATAAAAAA ACTTGCTTTA 
TTTGATGAAT GGAATTCCTT GGCTGTTTAT GTTTCAATGG ATAACACAGT GGTCATTGAA 
GATATCAAGA AAATGTGCTG TGTTCTTCCT GTGGGTGCTG CTGACACAGC TGGTGAGAGC 
CCTCCTGATT CTCTGATTGC TTCCAGTCAG AGTAAGGGCA CCTCTGCCCC GTGCCTAGCC 
TGGAAACCCC TGCTGCTCAT TGTGCCCCTT CGCCTGGGCA TAAACCAAAT CAACCCTGTG 
TATATTGAAG CATTCAAAGA GTGTTTTAAG ATGCCACAGT CTTTAGGGGC TTTAGGAGGA 
AAGCCAAATA ACGCCTATTA TTTCATAGGA TCCTTAGGTG ACGAGCTCAT TTTCTTGGAT 
CCTCACACAA CCCAGACCTT CGTTGATACT GAAGAGAGTG GACTAGTAGA CGACCACACT 
TTCCATTGCC TGCAGTCTCC ACAGCGGATG AGTATCCTGA ACTTGGATCC TTCTGTGGCC 
TTGGTGGGTC AAGGAGCTTT TATGGGATTT TTCTGCAAAG AAGAGAAAGA CTTTGATAAT 
TGGTGTAGTC TTGTTCAGAA GGAAATTCTA AAGGAGAATT TGAGGATGTT TGAATTAGTT 
CAGAAACATC CATCACACTG GCCTCCCTTT GTACCTCCAG CCAAGCCAGA AGTGACAACC 
ACAGGGGCAG AATTCATAGA CTCTACCGAA CAACTGGAGG ACTTCGACCT GGAGGAAGAT 
TTTGAGATTC TGAGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119770.1
CDS	31..1251
Translation	MESVMSKYENQIIIFTEYLEEFPDTDELVWILGKPHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWNWERQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVIKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCCVLPVGAADTAGESPPDSLIASSQSKGTSAPCLAWKPLLLIVPLRLGINQINPVYIEAFKECFKMPQSLGALGGKPNNAYYFIGSLGDELIFLDPHTTQTFVDTEESGLVDDHTFHCLQSPQRMSILNLDPSVALVGQGAFMGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEDFDLEEDFEILSV

Target ORF information:

RefSeq Version	NM_001126298.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy related 4A, cysteine peptidase (Atg4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126298.1

ORF Insert Sequence:

ATGGAGTCAG TTATGTCCAA GTATGAAAAC CAGATTATTA TTTTCACTGA ATACCTAGAA 
GAATTCCCAG ATACAGATGA GCTAGTATGG ATCCTGGGGA AGCCACATCT CCTTAAGACA 
GAAAAATCTA AGCTGTTGTC TGATATAAGT GCTCGTCTAT GGTTTACATA CAGAAGGAAA 
TTTTCACCGA TTGGGGGAAC AGGCCCTTCG TCGGATGCTG GCTGGGGATG TATGCTGCGC 
TGTGGGCAGA TGATGCTGGC TCAAGCCCTC ATCTGTAGAC ACTTGGGAAG GGATTGGAAC 
TGGGAGAGAC AAAAAGAACA ACCCAAAGAA TACCAAAGGA TACTGCAGTG TTTCCTAGAT 
AGAAAAGACT GTTGCTATTC AATCCATCAG ATGGCACAAA TGGGTGTAGG AGAAGGGAAA 
TCAATTGGCG AATGGTTTGG ACCAAATACA GTTGCACAGG TGATAAAAAA ACTTGCTTTA 
TTTGATGAAT GGAATTCCTT GGCTGTTTAT GTTTCAATGG ATAACACAGT GGTCATTGAA 
GATATCAAGA AAATGTGCTG TGTTCTTCCT GTGGGTGCTG CTGACACAGC TGGTGAGAGC 
CCTCCTGATT CTCTGATTGC TTCCAGTCAG AGTAAGGGCA CCTCTGCCCC GTGCCTAGCC 
TGGAAACCCC TGCTGCTCAT TGTGCCCCTT CGCCTGGGCA TAAACCAAAT CAACCCTGTG 
TATATTGAAG CATTCAAAGA GTGTTTTAAG ATGCCACAGT CTTTAGGGGC TTTAGGAGGA 
AAGCCAAATA ACGCCTATTA TTTCATAGGA TCCTTAGGTG ACGAGCTCAT TTTCTTGGAT 
CCTCACACAA CCCAGACCTT CGTTGATACT GAAGAGAGTG GACTAGTAGA CGACCACACT 
TTCCATTGCC TGCAGTCTCC ACAGCGGATG AGTATCCTGA ACTTGGATCC TTCTGTGGCC 
TTGGTGGGTC AAGGAGCTTT TATGGGATTT TTCTGCAAAG AAGAGAAAGA CTTTGATAAT 
TGGTGTAGTC TTGTTCAGAA GGAAATTCTA AAGGAGAATT TGAGGATGTT TGAATTAGTT 
CAGAAACATC CATCACACTG GCCTCCCTTT GTACCTCCAG CCAAGCCAGA AGTGACAACC 
ACAGGGGCAG AATTCATAGA CTCTACCGAA CAACTGGAGG ACTTCGACCT GGAGGAAGAT 
TTTGAGATTC TGAGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene and alternative splicing annotation with AIR.
Genome research15(1)54-66(2005 Jan)
Florea L,Di Francesco V,Miller J,Turner R,Yao A,Harris M,Walenz B,Mobarry C,Merkulov GV,Charlab R,Dew I,Deng Z,Istrail S,Li P,Sutton G

LC3 conjugation system in mammalian autophagy.
The international journal of biochemistry & cell biology36(12)2503-18(2004 Dec)
Tanida I,Ueno T,Kominami E

LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.
Journal of cell science117(Pt 13)2805-12(2004 Jun)
Kabeya Y,Mizushima N,Yamamoto A,Oshitani-Okamoto S,Ohsumi Y,Yoshimori T

The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A.
The Journal of biological chemistry278(16)14053-8(2003 Apr)
Scherz-Shouval R,Sagiv Y,Shorer H,Elazar Z