Pfkm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkm
Entrez Gene ID	65152
Full Name	phosphofructokinase, muscle
Synonyms	ATP-PFK,PFK-A,Pfk-M
General protein information	Preferred Names phosphofructokinase, muscle Names ATP-dependent 6-phosphofructokinase, muscle type 6-phosphofructokinase type A 6-phosphofructokinase, muscle type phosphofructo-1-kinase isozyme A phosphofructokinase 1 phosphofructokinase-A phosphofructokinase-M phosphohexokinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	phosphofructokinase isozyme A; may regulate glycolytic flux and influence insulin secretion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031715.1	NP_113903.1	ATP-dependent 6-phosphofructokinase, muscle type

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region.
Ismail R, Ul Hussain M
Biochimie13938-45(2017 Aug)

Aerobic glycolysis tunes YAP/TAZ transcriptional activity.
Enzo E, Santinon G, Pocaterra A, Aragona M, Bresolin S, Forcato M, Grifoni D, Pession A, Zanconato F, Guzzo G, Bicciato S, Dupont S
The EMBO journal34(10)1349-70(2015 May)

Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5.
Nakamura N, Mori C, Eddy EM
Biology of reproduction82(3)504-15(2010 Mar)

Phosphofructo-1-kinase deficiency leads to a severe cardiac and hematological disorder in addition to skeletal muscle glycogenosis.
Garc?a M, Pujol A, Ruzo A, Riu E, Ruberte J, Arb?s A, Seraf?n A, Albella B, Fel?u JE, Bosch F
PLoS genetics5(8)e1000615(2009 Aug)

Serotonin stimulates mouse skeletal muscle 6-phosphofructo-1-kinase through tyrosine-phosphorylation of the enzyme altering its intracellular localization.
Coelho WS, Costa KC, Sola-Penna M
Molecular genetics and metabolism92(4)364-70(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High phosphofructokinase expression is associated with glioma.
Title: The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region.

Coordinated expression of PKF-A/FBPase controls sperm cell glucose metabolism and sperm development and function.
Title: Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase.

The following Pfkm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13488	NM_031715.1 Latest version!	Rattus norvegicus phosphofructokinase, muscle (Pfkm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$461.30 $659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkm ( NM_031715.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113903.1 Rattus norvegicus phosphofructokinase, muscle (Pfkm), mRNA.

Price & Availability↑

CloneID	ORa13488
Clone ID Related Accession (Same CDS sequence)	NM_031715.1
Accession Version	NM_031715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U25651.1. On Jan 12, 2005 this sequence version replaced XM_346812.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U25651.1, FN433010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCATG AAGAGCATCA TGAAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTACGG TCAGGGCTGT GGTACGAGTT 
GGCATCTTCA CCGGGCTCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAT 
GGTGGAGAGC ACATCAGGGA GGCCACCTGG GAGAGCGTGT CCATGATGCT CCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGGG AGCGAGAAGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTTCGTTCA GAGTGGAGTG ACTTATTGAA TGACCTCCAG 
AAAGATGGGA AGATCACAGC CGAGGAGCGT ACAAAGTCCA GCTACCTGAA CATCGTTTTC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACTGACTCT 
GCCCTGCACC GCATTGTAGA GATCGTGGAC GCCATCACCA CCACCGCTCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCGTGTG GGGCCGACTG GGTTTTCATT CCCGAGTGTC CGCCAGATGA CGACTGGGAA 
GAACACCTTT GTCGCCGTCT CAGTGAGACA AGAACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTTGCTG AGGGTGCAAT CGACAAAAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TAAAGCGTCT TGGATATGAT ACCAGGGTCA CTGTTCTGGG ACATGTACAG 
AGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AATACGGCTG TGCGCCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGCGGGTTG 
CACACGGTGG CTGTGATGAA CGTGGGGGCC CCAGCTGCTG GAATGAATGC TGCAGTTCGC 
TCTACTGTGA GGATTGGCCT TATCCAAGGC AACCGAGTGC TGGTCGTGCA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAA GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTAC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTATAACATC CAGGGCCTGG TTATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCCGC CACGGTTTCC AATAATGTCC CAGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACCTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AGCGGCGAGT GTTTATCATC GAGACGATGG GTGGTTACTG TGGCTATCTG 
GCCACCATGG CAGGACTGGC GGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATCCGAG ATCTCCAGGT TAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAG TGCAACGAGA ACTACACTAC TGATTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAA CCCAACTCCC TTTGACAGGA ATTTTGCCAC CAAGATGGGT 
GCTAAGGCTA CGAATTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CTCCCGACTC AGGTTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTGA CTGAGCTGAA GGACCAGACA GATTTTGAGC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTGGCCA AGTACGAGAT TGATCTGGAC 
ACCTCTGACC ACGCCCACCT GGAGCACATT TCCAGGAAGC GGTCTGGAGA AGCTGCTGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113903.1
CDS	36..2378
Translation	MTHEEHHEAKTLGIGKAIAVLTSGGDAQGMNATVRAVVRVGIFTGLRVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEERTKSSYLNIVFLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNTAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKYNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGNPTPFDRNFATKMGAKATNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	NM_031715.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphofructokinase, muscle (Pfkm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031715.1

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGAAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTACGG TCAGGGCTGT GGTACGAGTT 
GGCATCTTCA CCGGGCTCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAT 
GGTGGAGAGC ACATCAGGGA GGCCACCTGG GAGAGCGTGT CCATGATGCT CCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGGG AGCGAGAAGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTTCGTTCA GAGTGGAGTG ACTTATTGAA TGACCTCCAG 
AAAGATGGGA AGATCACAGC CGAGGAGCGT ACAAAGTCCA GCTACCTGAA CATCGTTTTC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACTGACTCT 
GCCCTGCACC GCATTGTAGA GATCGTGGAC GCCATCACCA CCACCGCTCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCGTGTG GGGCCGACTG GGTTTTCATT CCCGAGTGTC CGCCAGATGA CGACTGGGAA 
GAACACCTTT GTCGCCGTCT CAGTGAGACA AGAACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTTGCTG AGGGTGCAAT CGACAAAAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TAAAGCGTCT TGGATATGAT ACCAGGGTCA CTGTTCTGGG ACATGTACAG 
AGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AATACGGCTG TGCGCCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGCGGGTTG 
CACACGGTGG CTGTGATGAA CGTGGGGGCC CCAGCTGCTG GAATGAATGC TGCAGTTCGC 
TCTACTGTGA GGATTGGCCT TATCCAAGGC AACCGAGTGC TGGTCGTGCA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAA GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTAC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTATAACATC CAGGGCCTGG TTATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCCGC CACGGTTTCC AATAATGTCC CAGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACCTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AGCGGCGAGT GTTTATCATC GAGACGATGG GTGGTTACTG TGGCTATCTG 
GCCACCATGG CAGGACTGGC GGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATCCGAG ATCTCCAGGT TAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAG TGCAACGAGA ACTACACTAC TGATTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAA CCCAACTCCC TTTGACAGGA ATTTTGCCAC CAAGATGGGT 
GCTAAGGCTA CGAATTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CTCCCGACTC AGGTTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTGA CTGAGCTGAA GGACCAGACA GATTTTGAGC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTGGCCA AGTACGAGAT TGATCTGGAC 
ACCTCTGACC ACGCCCACCT GGAGCACATT TCCAGGAAGC GGTCTGGAGA AGCTGCTGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region.
Biochimie13938-45(2017 Aug)
Ismail R,Ul Hussain M

Aerobic glycolysis tunes YAP/TAZ transcriptional activity.
The EMBO journal34(10)1349-70(2015 May)
Enzo E,Santinon G,Pocaterra A,Aragona M,Bresolin S,Forcato M,Grifoni D,Pession A,Zanconato F,Guzzo G,Bicciato S,Dupont S

Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5.
Biology of reproduction82(3)504-15(2010 Mar)
Nakamura N,Mori C,Eddy EM

Phosphofructo-1-kinase deficiency leads to a severe cardiac and hematological disorder in addition to skeletal muscle glycogenosis.
PLoS genetics5(8)e1000615(2009 Aug)
Garc?a M,Pujol A,Ruzo A,Riu E,Ruberte J,Arb?s A,Seraf?n A,Albella B,Fel?u JE,Bosch F

Serotonin stimulates mouse skeletal muscle 6-phosphofructo-1-kinase through tyrosine-phosphorylation of the enzyme altering its intracellular localization.
Molecular genetics and metabolism92(4)364-70(2007 Dec)
Coelho WS,Costa KC,Sola-Penna M