Cldn7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn7
Entrez Gene ID	65132
Full Name	claudin 7
General protein information	Preferred Names claudin 7 Names claudin-7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	mouse homolog is an integral membrane protein; component of tight junctions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246805.3	XP_006246867.1	claudin-7 isoform X1
NM_031702.1	NP_113890.1	claudin-7 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CLDN7	XP_546584.3
Mus musculus (house mouse)	Cldn7	NP_001180548.1
Rattus norvegicus (Norway rat)	Cldn7	NP_113890.1
Danio rerio (zebrafish)	cldn7a	NP_001002340.1
Danio rerio (zebrafish)	cldn7b	NP_571712.1
Pan troglodytes (chimpanzee)	CLDN7	XP_001169597.1
	CLDN7	XP_001107265.1
Homo sapiens (human)	CLDN7	NP_001171951.1
Bos taurus (cattle)	CLDN7	NP_001035609.1

Gene Ontology
Bibliography

Related articles in PubMed

Palmitoylated claudin7 captured in glycolipid-enriched membrane microdomains promotes metastasis via associated transmembrane and cytosolic molecules.
Thuma F, Heiler S, Schn?lzer M, Z?ller M
Oncotarget7(21)30659-77(2016 May)

EpCAM-associated claudin-7 supports lymphatic spread and drug resistance in rat pancreatic cancer.
Thuma F, Z?ller M
International journal of cancer133(4)855-66(2013 Aug)

Claudin 7 is reduced in uterine epithelial cells during early pregnancy in the rat.
Poon CE, Madawala RJ, Day ML, Murphy CR
Histochemistry and cell biology139(4)583-93(2013 Apr)

Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Guillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S
Journal of cell science125(Pt 21)5005-14(2012 Nov)

WNK4 phosphorylates ser(206) of claudin-7 and promotes paracellular Cl(-) permeability.
Tatum R, Zhang Y, Lu Q, Kim K, Jeansonne BG, Chen YH
FEBS letters581(20)3887-91(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pancreatic adenocarcinoma cell line ASMLL-claudin7 (cld7) knockdown (kd) and ASML-cld7 mutated palmitoylation cells show reduced motility and invasiveness.
Title: Palmitoylated claudin7 captured in glycolipid-enriched membrane microdomains promotes metastasis via associated transmembrane and cytosolic molecules.

cld7 supports tumorigenic features of EpC by provoking EpC cleavage and thereby its cotranscription factor activity
Title: EpCAM-associated claudin-7 supports lymphatic spread and drug resistance in rat pancreatic cancer.

claudin 7 demonstrates a distinct basal and lateral localisation in the uterine luminal and glandular epithelium throughout early pregnancy
Title: Claudin 7 is reduced in uterine epithelial cells during early pregnancy in the rat.

Claudin-7 in lateral membranes may exist in a nonpolymerized form and may play some different roles other than the formation of tight junctions strands, for example, in the formation of a pool of claudin proteins or in the reinforcement of cell adhesion.
Title: Claudin-7 expressed on lateral membrane of rat epididymal epithelium does not form aberrant tight junction strands.

Claudin-7, kidney injury molecule-1 (Kim-1), and matrix metalloproteinase-7 (MMP-7) changed by aging and changes attenuated by caloric restriction.
Title: Increased susceptibility of aging kidney to ischemic injury: identification of candidate genes changed during aging, but corrected by caloric restriction.

The following Cldn7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08517	NM_031702.1 Latest version!	Rattus norvegicus claudin 7 (Cldn7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa33380	XM_006246805.3 Latest version!	Rattus norvegicus claudin 7 (Cldn7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn7 ( NM_031702.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113890.1 Rattus norvegicus claudin 7 (Cldn7), mRNA.

Price & Availability↑

CloneID	ORa08517
Clone ID Related Accession (Same CDS sequence)	NM_031702.1
Accession Version	NM_031702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	claudin-7 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC101892.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC101892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCTAACT CGGGCCTGCA ACTACTGGGC TTTTCAATGG CCATGCTTGG CTGGGTGGGT 
CTGATAGCGA GCACTGCTAT CCCACAGTGG CAGATGAGCT CCTATGCAGG CGACAACATC 
ATCACAGCCC AGGCCATGTA CAAGGGGCTC TGGATGGAGT GCGTCACGCA GAGTACCGGC 
ATGATGAGCT GCAAAATGTA CGACTCGGTG CTTGCCCTGC CAGCGGCCAC GCAGGCCACT 
CGAGCCTTAA TGATTGTGTC CTTGGTGTTG GGCTTCTTGG CCATGTTTGT CGCCACGATG 
GGCATGAAAT GCACACGCTG TGGGGGAGAT GACAAAGTGA AGAAGGCCCG CATAGCTATG 
ACTGGAGGCA TTATTTTCAT CGTGGCAGGT CTTGCTGCTT TGGTAGCATG CTCCTGGATT 
GGTCATCAGA TTGTCACAGA CTTTTATAAC CCCTTGACGC CCATGAATAT TAAGTACGAG 
TTTGGTCCTG CCATCTTTAT CGGCTGGGCA GGGTCTGCTC TGGTCCTTCT GGGAGGGGCC 
CTGCTCTCTT GCTCCTGCCC CGGCAGTGAA AGCAAAGCTG CATACCGTGC ACCCCGCTCC 
TACCCTAAGT CCAACTCCTC TAAGGAATAC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113890.1
CDS	306..941
Translation	MANSGLQLLGFSMAMLGWVGLIASTAIPQWQMSSYAGDNIITAQAMYKGLWMECVTQSTGMMSCKMYDSVLALPAATQATRALMIVSLVLGFLAMFVATMGMKCTRCGGDDKVKKARIAMTGGIIFIVAGLAALVACSWIGHQIVTDFYNPLTPMNIKYEFGPAIFIGWAGSALVLLGGALLSCSCPGSESKAAYRAPRSYPKSNSSKEYV

Target ORF information:

RefSeq Version	NM_031702.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus claudin 7 (Cldn7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031702.1

ORF Insert Sequence:

ATGGCTAACT CGGGCCTGCA ACTACTGGGC TTTTCAATGG CCATGCTTGG CTGGGTGGGT 
CTGATAGCGA GCACTGCTAT CCCACAGTGG CAGATGAGCT CCTATGCAGG CGACAACATC 
ATCACAGCCC AGGCCATGTA CAAGGGGCTC TGGATGGAGT GCGTCACGCA GAGTACCGGC 
ATGATGAGCT GCAAAATGTA CGACTCGGTG CTTGCCCTGC CAGCGGCCAC GCAGGCCACT 
CGAGCCTTAA TGATTGTGTC CTTGGTGTTG GGCTTCTTGG CCATGTTTGT CGCCACGATG 
GGCATGAAAT GCACACGCTG TGGGGGAGAT GACAAAGTGA AGAAGGCCCG CATAGCTATG 
ACTGGAGGCA TTATTTTCAT CGTGGCAGGT CTTGCTGCTT TGGTAGCATG CTCCTGGATT 
GGTCATCAGA TTGTCACAGA CTTTTATAAC CCCTTGACGC CCATGAATAT TAAGTACGAG 
TTTGGTCCTG CCATCTTTAT CGGCTGGGCA GGGTCTGCTC TGGTCCTTCT GGGAGGGGCC 
CTGCTCTCTT GCTCCTGCCC CGGCAGTGAA AGCAAAGCTG CATACCGTGC ACCCCGCTCC 
TACCCTAAGT CCAACTCCTC TAAGGAATAC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cldn7 ( XM_006246805.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246867.1 Rattus norvegicus claudin 7 (Cldn7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33380
Clone ID Related Accession (Same CDS sequence)	XM_006246805.3
Accession Version	XM_006246805.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	claudin-7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACT CGGGCCTGCA ACTACTGGGC TTTTCAATGG CCATGCTTGG CTGGGTGGGT 
CTGATAGCGA GCACTGCTAT CCCACAGTGG CAGATGAGCT CCTATGCAGG CGACAACATC 
ATCACAGCCC AGGCCATGTA CAAGGGGCTC TGGATGGAGT GTGTCACGCA GAGTACCGGC 
ATGATGAGCT GCAAAATGTA CGACTCGGTG CTTGCCCTGC CAGCGGCCAC GCAGGCCACT 
CGAGCCTTAA TGATTGTGTC CTTGGTGTTG GGCTTCTTGG CCATGTTTGT CGCCACGATG 
GGCATGAAAT GCACACGCTG TGGGGGAGAT GACAAAGTGA AGAAGGCCCG CATAGCTATG 
ACTGGAGGCA TTATTTTCAT CGTGGCAGGT CTTGCTGCTT TGGTAGCATG CTCCTGGATT 
GGTCATCAGA TTGTCACAGA CTTTTATAAC CCCTTGACGC CCATGAATAT TAAGTACGAG 
TTTGGTCCTG CCATCTTTAT CGGCTGGGCA GGGTCTGCTC TGGTCCTTCT GGGAGGGGCC 
CTGCTCTCTT GCTCCTGCCC CGGCAGTGAA AGCAAAGCTG CATACCGTGC ACCCCGCTCC 
TACCCTAAGT CCAACTCCTC TAAGGAATAC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246867.1
CDS	502..1137
Translation	MANSGLQLLGFSMAMLGWVGLIASTAIPQWQMSSYAGDNIITAQAMYKGLWMECVTQSTGMMSCKMYDSVLALPAATQATRALMIVSLVLGFLAMFVATMGMKCTRCGGDDKVKKARIAMTGGIIFIVAGLAALVACSWIGHQIVTDFYNPLTPMNIKYEFGPAIFIGWAGSALVLLGGALLSCSCPGSESKAAYRAPRSYPKSNSSKEYV

Target ORF information:

RefSeq Version	XM_006246805.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus claudin 7 (Cldn7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246805.3

ORF Insert Sequence:

ATGGCTAACT CGGGCCTGCA ACTACTGGGC TTTTCAATGG CCATGCTTGG CTGGGTGGGT 
CTGATAGCGA GCACTGCTAT CCCACAGTGG CAGATGAGCT CCTATGCAGG CGACAACATC 
ATCACAGCCC AGGCCATGTA CAAGGGGCTC TGGATGGAGT GTGTCACGCA GAGTACCGGC 
ATGATGAGCT GCAAAATGTA CGACTCGGTG CTTGCCCTGC CAGCGGCCAC GCAGGCCACT 
CGAGCCTTAA TGATTGTGTC CTTGGTGTTG GGCTTCTTGG CCATGTTTGT CGCCACGATG 
GGCATGAAAT GCACACGCTG TGGGGGAGAT GACAAAGTGA AGAAGGCCCG CATAGCTATG 
ACTGGAGGCA TTATTTTCAT CGTGGCAGGT CTTGCTGCTT TGGTAGCATG CTCCTGGATT 
GGTCATCAGA TTGTCACAGA CTTTTATAAC CCCTTGACGC CCATGAATAT TAAGTACGAG 
TTTGGTCCTG CCATCTTTAT CGGCTGGGCA GGGTCTGCTC TGGTCCTTCT GGGAGGGGCC 
CTGCTCTCTT GCTCCTGCCC CGGCAGTGAA AGCAAAGCTG CATACCGTGC ACCCCGCTCC 
TACCCTAAGT CCAACTCCTC TAAGGAATAC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Palmitoylated claudin7 captured in glycolipid-enriched membrane microdomains promotes metastasis via associated transmembrane and cytosolic molecules.
Oncotarget7(21)30659-77(2016 May)
Thuma F,Heiler S,Schn?lzer M,Z?ller M

EpCAM-associated claudin-7 supports lymphatic spread and drug resistance in rat pancreatic cancer.
International journal of cancer133(4)855-66(2013 Aug)
Thuma F,Z?ller M

Claudin 7 is reduced in uterine epithelial cells during early pregnancy in the rat.
Histochemistry and cell biology139(4)583-93(2013 Apr)
Poon CE,Madawala RJ,Day ML,Murphy CR

Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Journal of cell science125(Pt 21)5005-14(2012 Nov)
Guillemot L,Schneider Y,Brun P,Castagliuolo I,Pizzuti D,Martines D,Jond L,Bongiovanni M,Citi S

WNK4 phosphorylates ser(206) of claudin-7 and promotes paracellular Cl(-) permeability.
FEBS letters581(20)3887-91(2007 Aug)
Tatum R,Zhang Y,Lu Q,Kim K,Jeansonne BG,Chen YH