Vps33a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps33a
Entrez Gene ID	65081
Full Name	VPS33A CORVET/HOPS core subunit
Synonyms	r-Vps33a
General protein information	Preferred Names VPS33A CORVET/HOPS core subunit Names vacuolar protein sorting-associated protein 33A vacuolar protein sorting 33 homolog A vacuolar protein sorting 33A vacuolar protein sorting protein 33a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	role in vesicle-mediated protein trafficking to lysosomal compartments [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022961.3	NP_075250.2	vacuolar protein sorting-associated protein 33A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	vps33a	NP_001093443.1
Drosophila melanogaster (fruit fly)	car	NP_001259707.1
Magnaporthe oryzae (rice blast fungus)	MGG_09914	XP_003710012.1
Arabidopsis thaliana (thale cress)	ATVPS33	NP_001154679.1
Oryza sativa (rice)	Os04g0223000	NP_001052270.1
Homo sapiens (human)	VPS33A	NP_075067.2
Pan troglodytes (chimpanzee)	VPS33A	XP_001167604.1
	VPS33A	XP_001098683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003203	XP_312907.4
Canis lupus familiaris (dog)	VPS33A	XP_534660.2
Gallus gallus (chicken)	VPS33A	XP_415153.1
Bos taurus (cattle)	VPS33A	NP_001039514.1
Rattus norvegicus (Norway rat)	Vps33a	NP_075250.2
Neurospora crassa	NCU09121	XP_958893.1
Mus musculus (house mouse)	Vps33a	NP_084205.3
Schizosaccharomyces pombe (fission yeast)	vps33	NP_596210.1
Xenopus tropicalis (tropical clawed frog)	vps33a	NP_001016935.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Kondo H, Maksimova N, Otomo T, Kato H, Imai A, Asano Y, Kobayashi K, Nojima S, Nakaya A, Hamada Y, Irahara K, Gurinova E, Sukhomyasova A, Nogovicina A, Savvina M, Yoshimori T, Ozono K, Sakai N
Human molecular genetics26(1)173-183(2017 01)

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.
Hubert V, Peschel A, Langer B, Gr?ger M, Rees A, Kain R
Biology open5(10)1516-1529(2016 Oct)

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Wartosch L, G?nesdogan U, Graham SC, Luzio JP
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Perini ED, Schaefer R, St?ter M, Kalaidzidis Y, Zerial M
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)

The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, Mizushima N
Molecular biology of the cell25(8)1327-37(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps33a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps33a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06549	NM_022961.3 Latest version!	Rattus norvegicus VPS33A core subunit of CORVET and HOPS complexes (Vps33a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps33a ( NM_022961.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075250.2 Rattus norvegicus VPS33A core subunit of CORVET and HOPS complexes (Vps33a), mRNA.

Price & Availability↑

CloneID	ORa06549
Clone ID Related Accession (Same CDS sequence)	NM_022961.3
Accession Version	NM_022961.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	vacuolar protein sorting-associated protein 33A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105752.1. On Aug 12, 2007 this sequence version replaced NM_022961.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105752.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTC ACCTGTCATA CGGGCGAGTG AACCTGAACG TGCTGCGCGA GGCGGTGCGT 
CGGGAGCTGC GCGAGTTTCT GGACAAGTGC GCTGGGAGCA AGGCAATAGT GTGGGATGAA 
TACTTAACAG GACCGTTTGG ACTGATTGCA CAGTACTCAC TACTAAAGGA ACATGAAGTG 
GAAAAAATGT TCACCCTCAA AGGAAGTCGT TTGCCAGCGG CTGATGTCAA GAATATCATT 
TTTTTGGTCA GACCCAGGCT AGAATTAATG GATATGATTG CCGAGAACGT ACTCAGTGAG 
GACAGGCGTG GCCCTACCAG GGATTTCCAT ATTTTGTTTG TGCCTCGTCG GAGCCTACTG 
TGTGAGCAAA GGCTGAAGGA CCTGGGTGTG TTGGGATCCT TTATTTATCG AGAGGAGTAC 
AGTTTAGATC TCATTCCATT TGATGGGGAC CTCTTATCCA TGGAGTCAGA GAGCGCTTTC 
AAAGAATGCT ACCTTGAAGG CGACCAGACA AGCCTGTACC ACGCAGCCAA GGGGCTGATG 
ACCCTGCAGG CGCTGTATGG AACTATCCCC CAGATCTTTG GGAAAGGAGA GTGTGCTCGG 
CAAGTGGCCA ATATGATGGT CAGGATGAAA AGGGAGTTTA CAGGCAGCCA AAACTCAGTG 
TTCCCCGTGT TTGATAACCT CTTACTGCTT GATCGCAACG TGGATCTGCT GACGCCGCTC 
GCCTCCCAGC TCACGTACGA AGGACTCATC GACGAGATTT ACGGCATTCA GAACAGTTAT 
GTGAAGTTAC CTCCAGAGAA ATTTGCACCC AAGAAGCAGG GCGGTGGTGG CGGGAAGGAC 
CTTCCCACAG AGGCAAAGAA GCTTCAGCTC AACTCCGCAG AGGAACTCTA TGCCGAGATC 
CGAGACAAGA ACTTCAACGC TGTGGGCTCC GTGCTCAGCA AGAAGGCGAA GATCATCTCC 
GCGGCCTTTG AGGAGCGACA CAATGCTAAG ACGGTTGGAG AGATCAAGCA ATTCGTCTCC 
CAGCTTCCCC ACATGCAGGC GGCCAGGGGG TCGCTTGCCA ACCACACCTC AATTGCAGAG 
TTAATCAAAG ACGTCACCAC TTCTGAAGAC TTCTTTGATA AGTTGACGGT GGAGCAGGAG 
TTCATGTCCG GGATAGACAC CGATAAGGTC AACAATTACA TCGAGGACTG CATTGCCCAA 
AAGCACCCGC TCATCAAGGT CCTGAGATTG GTTTGCCTCC AGTCTGTGTG CAACAGTGGG 
CTCAAGCAGA AGGTTCTGGA CTACTACAAA AGGGAAATTC TCCAGACCTA CGGCTATGAG 
CACATACTGA CCCTGAACAA CCTGGAGAAG GCCGGCCTGC TAAAGGCTCA GACAGGGGGC 
AGAAACAACT ACCCAACGAT TCGGAAAACA TTACGCCTCT GGATGGACGA TGTCAATGAA 
CAAAACCCCA CAGACATATC CTACGTGTAC AGTGGTTACG CACCACTCAG CGTGCGACTC 
GCCCAGCTCC TTTCTCGGCC AGGTTGGAGG AGCATCGAGG AGGTTCTCCG CATTCTCCCA 
GGGCCCCACT TTGAAGAAAG GCAGCCACTG CCCACCGGGC TGCAGAAGAA ACGCCAGCCA 
GGAGAAAACA GAGTCACACT GGTGTTTTTC CTCGGGGGCG TCACCTTTGC TGAAATCGCA 
GCCCTGCGTT TTCTCTCCCA GCTGGAAGAC GGAGGCACAG AGTATGTAAT TGCGACCACT 
AAACTGATAA ATGGAAGCAG CTGGCTAGAA GCTCTCATGG AAAAGCCTTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075250.2
CDS	17..1810
Translation	MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGSRLPAADVKNIIFLVRPRLELMDMIAENVLSEDRRGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIYREEYSLDLIPFDGDLLSMESESAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMVRMKREFTGSQNSVFPVFDNLLLLDRNVDLLTPLASQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGGGGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHPLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLNNLEKAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLVFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLINGSSWLEALMEKPF

Target ORF information:

RefSeq Version	NM_022961.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus VPS33A core subunit of CORVET and HOPS complexes (Vps33a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022961.3

ORF Insert Sequence:

ATGGCGGCTC ACCTGTCATA CGGGCGAGTG AACCTGAACG TGCTGCGCGA GGCGGTGCGT 
CGGGAGCTGC GCGAGTTTCT GGACAAGTGC GCTGGGAGCA AGGCAATAGT GTGGGATGAA 
TACTTAACAG GACCGTTTGG ACTGATTGCA CAGTACTCAC TACTAAAGGA ACATGAAGTG 
GAAAAAATGT TCACCCTCAA AGGAAGTCGT TTGCCAGCGG CTGATGTCAA GAATATCATT 
TTTTTGGTCA GACCCAGGCT AGAATTAATG GATATGATTG CCGAGAACGT ACTCAGTGAG 
GACAGGCGTG GCCCTACCAG GGATTTCCAT ATTTTGTTTG TGCCTCGTCG GAGCCTACTG 
TGTGAGCAAA GGCTGAAGGA CCTGGGTGTG TTGGGATCCT TTATTTATCG AGAGGAGTAC 
AGTTTAGATC TCATTCCATT TGATGGGGAC CTCTTATCCA TGGAGTCAGA GAGCGCTTTC 
AAAGAATGCT ACCTTGAAGG CGACCAGACA AGCCTGTACC ACGCAGCCAA GGGGCTGATG 
ACCCTGCAGG CGCTGTATGG AACTATCCCC CAGATCTTTG GGAAAGGAGA GTGTGCTCGG 
CAAGTGGCCA ATATGATGGT CAGGATGAAA AGGGAGTTTA CAGGCAGCCA AAACTCAGTG 
TTCCCCGTGT TTGATAACCT CTTACTGCTT GATCGCAACG TGGATCTGCT GACGCCGCTC 
GCCTCCCAGC TCACGTACGA AGGACTCATC GACGAGATTT ACGGCATTCA GAACAGTTAT 
GTGAAGTTAC CTCCAGAGAA ATTTGCACCC AAGAAGCAGG GCGGTGGTGG CGGGAAGGAC 
CTTCCCACAG AGGCAAAGAA GCTTCAGCTC AACTCCGCAG AGGAACTCTA TGCCGAGATC 
CGAGACAAGA ACTTCAACGC TGTGGGCTCC GTGCTCAGCA AGAAGGCGAA GATCATCTCC 
GCGGCCTTTG AGGAGCGACA CAATGCTAAG ACGGTTGGAG AGATCAAGCA ATTCGTCTCC 
CAGCTTCCCC ACATGCAGGC GGCCAGGGGG TCGCTTGCCA ACCACACCTC AATTGCAGAG 
TTAATCAAAG ACGTCACCAC TTCTGAAGAC TTCTTTGATA AGTTGACGGT GGAGCAGGAG 
TTCATGTCCG GGATAGACAC CGATAAGGTC AACAATTACA TCGAGGACTG CATTGCCCAA 
AAGCACCCGC TCATCAAGGT CCTGAGATTG GTTTGCCTCC AGTCTGTGTG CAACAGTGGG 
CTCAAGCAGA AGGTTCTGGA CTACTACAAA AGGGAAATTC TCCAGACCTA CGGCTATGAG 
CACATACTGA CCCTGAACAA CCTGGAGAAG GCCGGCCTGC TAAAGGCTCA GACAGGGGGC 
AGAAACAACT ACCCAACGAT TCGGAAAACA TTACGCCTCT GGATGGACGA TGTCAATGAA 
CAAAACCCCA CAGACATATC CTACGTGTAC AGTGGTTACG CACCACTCAG CGTGCGACTC 
GCCCAGCTCC TTTCTCGGCC AGGTTGGAGG AGCATCGAGG AGGTTCTCCG CATTCTCCCA 
GGGCCCCACT TTGAAGAAAG GCAGCCACTG CCCACCGGGC TGCAGAAGAA ACGCCAGCCA 
GGAGAAAACA GAGTCACACT GGTGTTTTTC CTCGGGGGCG TCACCTTTGC TGAAATCGCA 
GCCCTGCGTT TTCTCTCCCA GCTGGAAGAC GGAGGCACAG AGTATGTAAT TGCGACCACT 
AAACTGATAA ATGGAAGCAG CTGGCTAGAA GCTCTCATGG AAAAGCCTTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Human molecular genetics26(1)173-183(2017 01)
Kondo H,Maksimova N,Otomo T,Kato H,Imai A,Asano Y,Kobayashi K,Nojima S,Nakaya A,Hamada Y,Irahara K,Gurinova E,Sukhomyasova A,Nogovicina A,Savvina M,Yoshimori T,Ozono K,Sakai N

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.
Biology open5(10)1516-1529(2016 Oct)
Hubert V,Peschel A,Langer B,Gr?ger M,Rees A,Kain R

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)
Wartosch L,G?nesdogan U,Graham SC,Luzio JP

Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Traffic (Copenhagen, Denmark)15(12)1366-89(2014 Dec)
Perini ED,Schaefer R,St?ter M,Kalaidzidis Y,Zerial M

The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Molecular biology of the cell25(8)1327-37(2014 Apr)
Jiang P,Nishimura T,Sakamaki Y,Itakura E,Hatta T,Natsume T,Mizushima N