Map1lc3b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map1lc3b
Entrez Gene ID	64862
Full Name	microtubule-associated protein 1 light chain 3 beta
Synonyms	LC3B,Map1lc3,Mpl3,zbs559
General protein information	Preferred Names microtubule-associated protein 1 light chain 3 beta Names microtubule-associated proteins 1A/1B light chain 3B MAP1 light chain 3-like protein 2 MAP1A/1B light chain 3 B MAP1A/MAP1B LC3 B MAP1A/MAP1B light chain 3 B autophagy-related protein LC3 B autophagy-related ubiquitin-like modifier LC3 B microtubule-associated proteins 1A/1B light chain 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	may mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022867.2	NP_074058.2	microtubule-associated proteins 1A/1B light chain 3B
XM_017601351.1	XP_017456840.1	microtubule-associated proteins 1A/1B light chain 3B isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP1LC3B	NP_073729.1
Mus musculus (house mouse)	Map1lc3b	NP_080436.1
Danio rerio (zebrafish)	map1lc3b	NP_955898.1
Gallus gallus (chicken)	MAP1LC3B	NP_001026632.1
Xenopus tropicalis (tropical clawed frog)	map1lc3b	NP_989346.1
Pan troglodytes (chimpanzee)	MAP1LC3B	NP_001233505.1
	LOC697790	XP_001086333.2
Canis lupus familiaris (dog)	MAP1LC3B	XP_536756.2
Bos taurus (cattle)	MAP1LC3B	NP_001001169.1
Rattus norvegicus (Norway rat)	Map1lc3b	NP_074058.2
	MAP1LC3B	XP_002802632.1

Gene Ontology
Bibliography

Related articles in PubMed

Human CLEC16A regulates autophagy through modulating mTOR activity.
Tam RC, Li MW, Gao YP, Pang YT, Yan S, Ge W, Lau CS, Chan VS
Experimental cell research352(2)304-312(2017 03)

Hyperoxia-mediated LC3B activation contributes to the impaired transdifferentiation of type II alveolar epithelial cells (AECIIs) to type I cells (AECIs).
Zhang L, Zhao S, Yuan L, Wu H, Jiang H, Luo G
Clinical and experimental pharmacology & physiology43(9)834-43(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel role of activated LC3B induced by hyperoxia in AECII transdifferentiation
Title: Hyperoxia-mediated LC3B activation contributes to the impaired transdifferentiation of type II alveolar epithelial cells (AECIIs) to type I cells (AECIs).

Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3.
Title: Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3.

microtubule-associated protein 1 light chain 3 binds to cardiolipin. Preventing this interaction inhibits rotenone-induced mitochondrial delivery to autophagosomes
Title: LC3 binds externalized cardiolipin on injured mitochondria to signal mitophagy in neurons: implications for Parkinson disease.

Data show that caldendrin specifically associates with the microtubule cytoskeleton via an interaction with the microtubule componentlight chain 3 (Mpl3).
Title: Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins.

The following Map1lc3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map1lc3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06405	NM_022867.2 Latest version!	Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa59475	XM_017601351.1 Latest version!	Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map1lc3b ( NM_022867.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074058.2 Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), mRNA.

Price & Availability↑

CloneID	ORa06405
Clone ID Related Accession (Same CDS sequence)	NM_022867.2
Accession Version	NM_022867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated proteins 1A/1B light chain 3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY392036.1. On Jan 21, 2004 this sequence version replaced NM_022867.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY392036.1, BC083556.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTCCG AGAAGACCTT CAAACAGCGC CGGAGCTTCG AACAAAGAGT GGAAGATGTC 
CGGCTCATCC GGGAGCAGCA CCCCACCAAG ATCCCAGTGA TTATAGAGCG ATACAAGGGT 
GAGAAGCAGC TGCCCGTCCT GGACAAGACC AAGTTCCTTG TACCTGATCA CGTGAATATG 
AGCGAACTCA TCAAGATAAT TAGAAGGCGC CTGCAGCTCA ATGCTAACCA AGCCTTCTTC 
CTCCTGGTGA ATGGGCACAG CATGGTGAGT GTGTCCACAC CCATCTCTGA AGTGTACGAG 
AGCGAGAGAG ATGAAGACGG CTTCCTGTAC ATGGTCTATG CCTCCCAGGA GACGTTCGGG 
ACAGCACTGG CTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074058.2
CDS	77..454
Translation	MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTALAV

Target ORF information:

RefSeq Version	NM_022867.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022867.2

ORF Insert Sequence:

ATGCCGTCCG AGAAGACCTT CAAACAGCGC CGGAGCTTCG AACAAAGAGT GGAAGATGTC 
CGGCTCATCC GGGAGCAGCA CCCCACCAAG ATCCCAGTGA TTATAGAGCG ATACAAGGGT 
GAGAAGCAGC TGCCCGTCCT GGACAAGACC AAGTTCCTTG TACCTGATCA CGTGAATATG 
AGCGAACTCA TCAAGATAAT TAGAAGGCGC CTGCAGCTCA ATGCTAACCA AGCCTTCTTC 
CTCCTGGTGA ATGGGCACAG CATGGTGAGT GTGTCCACAC CCATCTCTGA AGTGTACGAG 
AGCGAGAGAG ATGAAGACGG CTTCCTGTAC ATGGTCTATG CCTCCCAGGA GACGTTCGGG 
ACAGCACTGG CTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map1lc3b ( XM_017601351.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456840.1 Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59475
Clone ID Related Accession (Same CDS sequence)	XM_017601351.1
Accession Version	XM_017601351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated proteins 1A/1B light chain 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG AGAAGACCTT CAAACAGCGC CGGAGCTTCG AACAAAGAGT GGAAGATGTC 
CGGCTCATCC GGGAGCAGCA CCCCACCAAG ATCCCAGTGA TTATAGAGCG ATACAAGGGT 
GAGAAGCAGC TGCCCGTCCT GGACAAGACC AAGTTCCTTG TACCTGATCA CGTGAATATG 
AGCGAACTCA TCAAGATAAT TAGAAGGCGC CTGCAGCTCA ATGCTAACCA AGCCTTCTTC 
CTCCTGGTGA ATGGGCACAG CATGGTGAGT GTGTCCACAC CCATCTCTGA AGTGTACGAG 
AGCGAGAGAG ATGAAGACGG CTTCCTGTAC ATGGTCTATG CCTCCCAGGA GACGTTCGGG 
ACAGCACTGG CTGTTACATA CATGTCAGCT CTGAAGGCAA CAGCAACAGG AAGAGAGCCA 
TGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456840.1
CDS	261..689
Translation	MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTALAVTYMSALKATATGREPCL

Target ORF information:

RefSeq Version	XM_017601351.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein 1 light chain 3 beta (Map1lc3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601351.1

ORF Insert Sequence:

ATGCCGTCCG AGAAGACCTT CAAACAGCGC CGGAGCTTCG AACAAAGAGT GGAAGATGTC 
CGGCTCATCC GGGAGCAGCA CCCCACCAAG ATCCCAGTGA TTATAGAGCG ATACAAGGGT 
GAGAAGCAGC TGCCCGTCCT GGACAAGACC AAGTTCCTTG TACCTGATCA CGTGAATATG 
AGCGAACTCA TCAAGATAAT TAGAAGGCGC CTGCAGCTCA ATGCTAACCA AGCCTTCTTC 
CTCCTGGTGA ATGGGCACAG CATGGTGAGT GTGTCCACAC CCATCTCTGA AGTGTACGAG 
AGCGAGAGAG ATGAAGACGG CTTCCTGTAC ATGGTCTATG CCTCCCAGGA GACGTTCGGG 
ACAGCACTGG CTGTTACATA CATGTCAGCT CTGAAGGCAA CAGCAACAGG AAGAGAGCCA 
TGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human CLEC16A regulates autophagy through modulating mTOR activity.
Experimental cell research352(2)304-312(2017 03)
Tam RC,Li MW,Gao YP,Pang YT,Yan S,Ge W,Lau CS,Chan VS

Hyperoxia-mediated LC3B activation contributes to the impaired transdifferentiation of type II alveolar epithelial cells (AECIIs) to type I cells (AECIs).
Clinical and experimental pharmacology & physiology43(9)834-43(2016 Sep)
Zhang L,Zhao S,Yuan L,Wu H,Jiang H,Luo G