Ireb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ireb2
Entrez Gene ID	64831
Full Name	iron responsive element binding protein 2
Synonyms	Irp2
General protein information	Preferred Names iron responsive element binding protein 2 Names iron-responsive element-binding protein 2 IRE-BP 2 iron regulatory protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	RNA-binding proteins that bind iron-responsive elements (IREs) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243091.3	XP_006243153.2	iron-responsive element-binding protein 2 isoform X1
NM_022863.2	NP_074054.2	iron-responsive element-binding protein 2

Pathways from BioSystems

Homologies

	IREB2	XP_001107837.1
Mus musculus (house mouse)	Ireb2	NP_073146.2
Rattus norvegicus (Norway rat)	Ireb2	NP_074054.2
Pan troglodytes (chimpanzee)	IREB2	XP_523125.2
Bos taurus (cattle)	IREB2	XP_002696712.2
Gallus gallus (chicken)	IREB2	NP_001026625.1
Danio rerio (zebrafish)	ireb2	XP_005163029.1
Homo sapiens (human)	IREB2	NP_004127.1
Xenopus tropicalis (tropical clawed frog)	ireb2	NP_001072764.1
Canis lupus familiaris (dog)	IREB2	XP_532364.2

Gene Ontology
Bibliography

Related articles in PubMed

Hypobaric Hypoxia Regulates Brain Iron Homeostasis in Rats.
Li Y, Yu P, Chang SY, Wu Q, Yu P, Xie C, Wu W, Zhao B, Gao G, Chang YZ
Journal of cellular biochemistry118(6)1596-1605(2017 Jun)

Insights into the role of iron in immature rat model of hypoxic-ischemic brain injury.
Wang ZW, Yang LJ, Ding YX, Chang YZ, Cui H
Experimental and therapeutic medicine12(3)1723-1731(2016 Sep)

Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model.
Zhang Y, Sun X, Xie C, Shu X, Oso AO, Ruan Z, Deng ZY, Wu X, Yin Y
Genes & nutrition10(6)54(2015 Nov)

Deficiency of Calcium-Independent Phospholipase A2 Beta Induces Brain Iron Accumulation through Upregulation of Divalent Metal Transporter 1.
Beck G, Shinzawa K, Hayakawa H, Baba K, Yasuda T, Sumi-Akamaru H, Tsujimoto Y, Mochizuki H
PloS one10(10)e0141629(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the genetic ablation of iPLA2b increased iron uptake in the brain through the activation of IRP2 and upregulation of DMT1, which may be associated with mitochondrial dysfunction.
Title: Deficiency of Calcium-Independent Phospholipase A2 Beta Induces Brain Iron Accumulation through Upregulation of Divalent Metal Transporter 1.

Vitamin A is involved in the regulation of IRP2, subsequently affecting iron metabolism gene expressions, such as Ferritin and transferrin receptor.
Title: Vitamin A deficiency aggravates iron deficiency by upregulating the expression of iron regulatory protein-2.

reversible phosphorylation of IRP2 during G(2)/M has a role in modulating the iron-independent expression of ferritin and other IRE-containing mRNAs during the cell cycle.
Title: Iron-independent phosphorylation of iron regulatory protein 2 regulates ferritin during the cell cycle.

IRP-2 serves as an important regulator of iron metabolism, and regulates iron uptake from the intestine by controlling the expression of ferritin mRNA at the post transcriptional level.
Title: [Expression of iron regulatory protein-2 and ferritins in intestinal mucosa of rats with iron deficiency].

The following Ireb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ireb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06395	NM_022863.2 Latest version!	Rattus norvegicus iron responsive element binding protein 2 (Ireb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa62540	XM_006243091.3 Latest version!	Rattus norvegicus iron responsive element binding protein 2 (Ireb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ireb2 ( NM_022863.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074054.2 Rattus norvegicus iron responsive element binding protein 2 (Ireb2), mRNA.

Price & Availability↑

CloneID	ORa06395
Clone ID Related Accession (Same CDS sequence)	NM_022863.2
Accession Version	NM_022863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	iron-responsive element-binding protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081798.1. On Jun 10, 2007 this sequence version replaced NM_022863.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081798.1, U20181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCC CAAGTGCAGG ATACACCTTT GAGTACCTTA TTGAAACATT AAATGGCAGT 
TCACAGAAGA AGTTCTTCAA TGTACCTAAA CTTGGAGGCA CCAAGTATGA TATTCTGCCT 
TACTCAATAC GGGTCCTATT GGAAGCTGCT GTACGAAATT GTGATGGATT TTTAATGAAA 
AAGGAAGATG TTATAAATAT TTTGGACTGG AAAACCAAAC AAAGCAATGT TGAAGTGCCC 
TTTTTCCCCG CCCGTGTTGT TCTTCAAGAT TTCACTGGAA TACCGGCAAT GGTGGATTTT 
GCTGCTATGA GGGAGGCAGT GAAAACTCTT GGAGGTGATC CTAAGAAAGT CCACCCTGCC 
TGTCCAACAG ATCTCACAGT TGACCACTCT TTACAGATTG ACTTCAGTAA ATGTGCAATA 
CAGAATGCAC CAAATCCTGG AGGTGGTGAC CTACAGAAAG CAGGAAAGCT CTCTCCACTT 
AAAGTACAGC CTAAGAAGCT TCCATGTCGA GGCCAGACTA CCTGCCGGGG ATCGTGTGAT 
TCTGGAGAAC TAAGCCGAAA CTCAGGAACA TTTTCTTCGC AGATTGAGAA TACGCCTGTC 
CTGTGTCCCT TTCATTTGCA ACCAGTGCCT GAACCTGAGA CGGTGTTAAA AAATCAAGAA 
GTAGAATTTG GCAGAAATCG AGAGAGGCTT CAATTTTTCA AGTGGAGCTC AGGAGCTTTT 
AAGAATGTGG CAGTCATCCC TCCTGGAACT GGAATGGCTC ATCAAGTGAA CTTAGAACAT 
TTGTCGAGAG TAGTGTTTGA AGAAGCCGAC CTGCTCTTCC CAGACAGCGT AATTGGCACA 
GATTCTCATA TAACCATGGT GAATGGATTG GGAATTCTTG GGTGGGGAGT TGGAGGCATT 
GAGACAGAGG CAGTTATGCT TGGCCTGCCA GTTACTCTTA CTTTACCAGA GGTGGTTGGA 
TGTGAGCTAA CTGGGTCATC CAATGCTTTT GTTACATCCA TAGATATTGT CCTAGGCATT 
ACAAAGCACC TCAGGCAAGT AGGCGTGGCT GGAAAGTTTG TTGAGTTCTT TGGAAGTGGA 
GTTTCACAAT TATCTATTGT CGATAGAACT ACTATAGCAA ACATGTGTCC CGAGTATGGT 
GCTATCCTCA GCTTTTTCCC TGTTGACAAT GTGACACTAC GACATTTAGA ACATACAGGT 
TTTGACAAAA CCAAACTTGA GTCAATGGAA GAATACCTTA AAGCTGTGAA ATTGTTTCGA 
AATGATGAGA ATTCTTCAGA ACCTGAATAT TCTCAGGTGA TACAGATTAA TCTGAATTCA 
ATAGTTGCAT CTGTCAGTGG TCCAAAAAGG CCTCAGGATA GAGTTGCTGT AACAGATATG 
AAAAGTGATT TTCAGGCTTG CTTAAATGAA AAGGTTGGAT TTAAAGGCTT CCAAGTTGCA 
GCAGAAAAAC AAAGTGATAC TGTCTCAGTT CGTTATGATG GAAGTGAGTA TAAGCTGTCC 
CATGGGTCCG TGGTCATTGC TGCGGTTATC AGCTGTACCA ATAACTGCAA TCCATCGGTG 
ATGCTCGCTG CAGGTCTTTT GGCTAAAAAG GCTGTTGAAA CTGGTCTACG AGTTAAACCT 
TATATAAGAA CAAGTTTGTC TCCAGGCAGT GGGATGGTTA CACATTACCT CAGTTCAAGT 
GGAGTGTTAC CCTACCTTAG CAAGCTAGGG TTTGAAATAG TTGGCTATGG ATGTTCAACG 
TGTGTAGGAA ATACAGCACC TTTATCAGAA GCAATTTTGA ATGCAGTAAA ACAGGGTGAT 
TTGGTTACCT GTGGAGTTTT ATCTGGAAAC AAAAATTTCG AAGGTCGTCT TTGTGATTGT 
GTCCGTGCTA ATTATCTCGC CTCTCCACCC TTAGTGGTGG CTTACGCCAT AGCAGGCACA 
GTGAATATAG ATTTCCAGAC AGAGCCATTA GGTACTGACT CTACAGGCAA GAACATTTAC 
CTGCATGACA TTTGGCCTAG TCGAGAAGAA GTTCATCAGA TAGAAGAAGA ACATGTTATT 
TTGTCCATGT TTAAAGCACT GAAAGAGAAA GTAGAGATGG GAAATAAACG GTGGAATTCC 
TTAGACGCTC CAGATTCAGT GTTGTTTCCA TGGGATGTTA AGTCTACTTA CATCAGATGC 
CCTTCATTTT TTGATAAACT TACCAAAGAA CCAGCTGCAT CCCAGCCTAT TGAAAATGCC 
CATGTCCTGT TGTACTTGGG AGACTCTGTC ACTACAGACC ACATATCACC TGCTGGAAGC 
ATTGCTAGGA GCAGTGCTGC TGCTAAGTAC TTGACAAACA GAGGCCTTAC TCCCCGAGAG 
TTCAACTCTT ATGGAGCTCG AAGAGGTAAT GATGCTGTGA TGACAAGAGG CACGTTTGCA 
AACATCAAGC TTTTTAATAA GTTTATTGGG AAGCCAGCTC CCAAAACAAT TCATTTTCCA 
TCAGGACAGA CGCTCGATGT ATTTGAAGCT GCAGAATTAT ACCAAAAAGA AGGTATCCCA 
CTGATAATTT TAGCAGGAAA AAAATATGGT TCAGGAAATT CAAGAGACTG GGCTGCGAAA 
GGACCCTATT TGCTGGGTGT AAAGGCTGTT TTGGCTGAAA GCTATGAAAA GATACACAAA 
GATCATTTGA TTGGAATTGG TATAGCACCA CTTGAGTTCC TCCCAGGAGA AAATGCAGAT 
TCCTTGGGCC TCTCCGGCAG AGAAGTATTT TCTTTATCAT TTCCTGAAGA ACTCTTTCCT 
GGAATTACGT TAAATATAAA GACGAGCACT GGCAAAGAGT TCAGCGTGAT TGCAGCATTT 
GAAAATGATG TGGAGATAAC TTTGTACAAA CATGGAGGAT TGTTAAACTT TGTGGCTCGA 
AAATTCTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074054.2
CDS	107..2998
Translation	MDSPSAGYTFEYLIETLNGSSQKKFFNVPKLGGTKYDILPYSIRVLLEAAVRNCDGFLMKKEDVINILDWKTKQSNVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELSRNSGTFSSQIENTPVLCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSGAFKNVAVIPPGTGMAHQVNLEHLSRVVFEEADLLFPDSVIGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVTLTLPEVVGCELTGSSNAFVTSIDIVLGITKHLRQVGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLRHLEHTGFDKTKLESMEEYLKAVKLFRNDENSSEPEYSQVIQINLNSIVASVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQVAAEKQSDTVSVRYDGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVETGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEIVGYGCSTCVGNTAPLSEAILNAVKQGDLVTCGVLSGNKNFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFQTEPLGTDSTGKNIYLHDIWPSREEVHQIEEEHVILSMFKALKEKVEMGNKRWNSLDAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVFSLSFPEELFPGITLNIKTSTGKEFSVIAAFENDVEITLYKHGGLLNFVARKFL

Target ORF information:

RefSeq Version	NM_022863.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iron responsive element binding protein 2 (Ireb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022863.2

ORF Insert Sequence:

ATGGACTCCC CAAGTGCAGG ATACACCTTT GAGTACCTTA TTGAAACATT AAATGGCAGT 
TCACAGAAGA AGTTCTTCAA TGTACCTAAA CTTGGAGGCA CCAAGTATGA TATTCTGCCT 
TACTCAATAC GGGTCCTATT GGAAGCTGCT GTACGAAATT GTGATGGATT TTTAATGAAA 
AAGGAAGATG TTATAAATAT TTTGGACTGG AAAACCAAAC AAAGCAATGT TGAAGTGCCC 
TTTTTCCCCG CCCGTGTTGT TCTTCAAGAT TTCACTGGAA TACCGGCAAT GGTGGATTTT 
GCTGCTATGA GGGAGGCAGT GAAAACTCTT GGAGGTGATC CTAAGAAAGT CCACCCTGCC 
TGTCCAACAG ATCTCACAGT TGACCACTCT TTACAGATTG ACTTCAGTAA ATGTGCAATA 
CAGAATGCAC CAAATCCTGG AGGTGGTGAC CTACAGAAAG CAGGAAAGCT CTCTCCACTT 
AAAGTACAGC CTAAGAAGCT TCCATGTCGA GGCCAGACTA CCTGCCGGGG ATCGTGTGAT 
TCTGGAGAAC TAAGCCGAAA CTCAGGAACA TTTTCTTCGC AGATTGAGAA TACGCCTGTC 
CTGTGTCCCT TTCATTTGCA ACCAGTGCCT GAACCTGAGA CGGTGTTAAA AAATCAAGAA 
GTAGAATTTG GCAGAAATCG AGAGAGGCTT CAATTTTTCA AGTGGAGCTC AGGAGCTTTT 
AAGAATGTGG CAGTCATCCC TCCTGGAACT GGAATGGCTC ATCAAGTGAA CTTAGAACAT 
TTGTCGAGAG TAGTGTTTGA AGAAGCCGAC CTGCTCTTCC CAGACAGCGT AATTGGCACA 
GATTCTCATA TAACCATGGT GAATGGATTG GGAATTCTTG GGTGGGGAGT TGGAGGCATT 
GAGACAGAGG CAGTTATGCT TGGCCTGCCA GTTACTCTTA CTTTACCAGA GGTGGTTGGA 
TGTGAGCTAA CTGGGTCATC CAATGCTTTT GTTACATCCA TAGATATTGT CCTAGGCATT 
ACAAAGCACC TCAGGCAAGT AGGCGTGGCT GGAAAGTTTG TTGAGTTCTT TGGAAGTGGA 
GTTTCACAAT TATCTATTGT CGATAGAACT ACTATAGCAA ACATGTGTCC CGAGTATGGT 
GCTATCCTCA GCTTTTTCCC TGTTGACAAT GTGACACTAC GACATTTAGA ACATACAGGT 
TTTGACAAAA CCAAACTTGA GTCAATGGAA GAATACCTTA AAGCTGTGAA ATTGTTTCGA 
AATGATGAGA ATTCTTCAGA ACCTGAATAT TCTCAGGTGA TACAGATTAA TCTGAATTCA 
ATAGTTGCAT CTGTCAGTGG TCCAAAAAGG CCTCAGGATA GAGTTGCTGT AACAGATATG 
AAAAGTGATT TTCAGGCTTG CTTAAATGAA AAGGTTGGAT TTAAAGGCTT CCAAGTTGCA 
GCAGAAAAAC AAAGTGATAC TGTCTCAGTT CGTTATGATG GAAGTGAGTA TAAGCTGTCC 
CATGGGTCCG TGGTCATTGC TGCGGTTATC AGCTGTACCA ATAACTGCAA TCCATCGGTG 
ATGCTCGCTG CAGGTCTTTT GGCTAAAAAG GCTGTTGAAA CTGGTCTACG AGTTAAACCT 
TATATAAGAA CAAGTTTGTC TCCAGGCAGT GGGATGGTTA CACATTACCT CAGTTCAAGT 
GGAGTGTTAC CCTACCTTAG CAAGCTAGGG TTTGAAATAG TTGGCTATGG ATGTTCAACG 
TGTGTAGGAA ATACAGCACC TTTATCAGAA GCAATTTTGA ATGCAGTAAA ACAGGGTGAT 
TTGGTTACCT GTGGAGTTTT ATCTGGAAAC AAAAATTTCG AAGGTCGTCT TTGTGATTGT 
GTCCGTGCTA ATTATCTCGC CTCTCCACCC TTAGTGGTGG CTTACGCCAT AGCAGGCACA 
GTGAATATAG ATTTCCAGAC AGAGCCATTA GGTACTGACT CTACAGGCAA GAACATTTAC 
CTGCATGACA TTTGGCCTAG TCGAGAAGAA GTTCATCAGA TAGAAGAAGA ACATGTTATT 
TTGTCCATGT TTAAAGCACT GAAAGAGAAA GTAGAGATGG GAAATAAACG GTGGAATTCC 
TTAGACGCTC CAGATTCAGT GTTGTTTCCA TGGGATGTTA AGTCTACTTA CATCAGATGC 
CCTTCATTTT TTGATAAACT TACCAAAGAA CCAGCTGCAT CCCAGCCTAT TGAAAATGCC 
CATGTCCTGT TGTACTTGGG AGACTCTGTC ACTACAGACC ACATATCACC TGCTGGAAGC 
ATTGCTAGGA GCAGTGCTGC TGCTAAGTAC TTGACAAACA GAGGCCTTAC TCCCCGAGAG 
TTCAACTCTT ATGGAGCTCG AAGAGGTAAT GATGCTGTGA TGACAAGAGG CACGTTTGCA 
AACATCAAGC TTTTTAATAA GTTTATTGGG AAGCCAGCTC CCAAAACAAT TCATTTTCCA 
TCAGGACAGA CGCTCGATGT ATTTGAAGCT GCAGAATTAT ACCAAAAAGA AGGTATCCCA 
CTGATAATTT TAGCAGGAAA AAAATATGGT TCAGGAAATT CAAGAGACTG GGCTGCGAAA 
GGACCCTATT TGCTGGGTGT AAAGGCTGTT TTGGCTGAAA GCTATGAAAA GATACACAAA 
GATCATTTGA TTGGAATTGG TATAGCACCA CTTGAGTTCC TCCCAGGAGA AAATGCAGAT 
TCCTTGGGCC TCTCCGGCAG AGAAGTATTT TCTTTATCAT TTCCTGAAGA ACTCTTTCCT 
GGAATTACGT TAAATATAAA GACGAGCACT GGCAAAGAGT TCAGCGTGAT TGCAGCATTT 
GAAAATGATG TGGAGATAAC TTTGTACAAA CATGGAGGAT TGTTAAACTT TGTGGCTCGA 
AAATTCTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ireb2 ( XM_006243091.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243153.2 Rattus norvegicus iron responsive element binding protein 2 (Ireb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62540
Clone ID Related Accession (Same CDS sequence)	XM_006243091.3
Accession Version	XM_006243091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	iron-responsive element-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGATGTTAT AAATATTTTG GACTGGAAAA CCAAACAAAG CAATGTTGAA 
GTGCCCTTTT TCCCCGCCCG TGTTGTTCTT CAAGATTTCA CTGGAATACC GGCAATGGTG 
GATTTTGCTG CTATGAGGGA GGCAGTGAAA ACTCTTGGAG GTGATCCTAA GAAAGTCCAC 
CCTGCCTGTC CAACAGATCT CACAGTTGAC CACTCTTTAC AGATTGACTT CAGTAAATGT 
GCAATACAGA ATGCACCAAA TCCTGGAGGT GGTGACCTAC AGAAAGCAGG AAAGCTCTCT 
CCACTTAAAG TACAGCCTAA GAAGCTTCCA TGTCGAGGCC AGACTACCTG CCGGGGATCG 
TGTGATTCTG GAGAACTAAG CCGAAACTCA GGAACATTTT CTTCGCAGAT TGAGAATACG 
CCTGTCCTGT GTCCCTTTCA TTTGCAACCA GTGCCTGAAC CTGAGACGGT GTTAAAAAAT 
CAAGAAGTAG AATTTGGCAG AAATCGAGAG AGGCTTCAAT TTTTCAAGTG GAGCTCAGGA 
GCTTTTAAGA ATGTGGCAGT CATCCCTCCT GGAACTGGAA TGGCTCATCA AGTGAACTTA 
GAACATTTGT CGAGAGTAGT GTTTGAAGAA GCCGACCTGC TCTTCCCAGA CAGCGTAATT 
GGCACAGATT CTCATATAAC CATGGTGAAT GGATTGGGAA TTCTTGGGTG GGGAGTTGGA 
GGCATTGAGA CAGAGGCAGT TATGCTTGGC CTGCCAGTTA CTCTTACTTT ACCAGAGGTG 
GTTGGATGTG AGCTAACTGG GTCATCCAAT GCTTTTGTTA CATCCATAGA TATTGTCCTA 
GGCATTACAA AGCACCTCAG GCAAGTAGGC GTGGCTGGAA AGTTTGTTGA GTTCTTTGGA 
AGTGGAGTTT CACAATTATC TATTGTCGAT AGAACTACTA TAGCAAACAT GTGTCCCGAG 
TATGGTGCTA TCCTCAGCTT TTTCCCTGTT GACAATGTGA CACTACGACA TTTAGAACAT 
ACAGGTTTTG ACAAAACCAA ACTTGAGTCA ATGGAAGAAT ACCTTAAAGC TGTGAAATTG 
TTTCGAAATG ATGAGAATTC TTCAGAACCT GAATATTCTC AGGTGATACA GATTAATCTG 
AATTCAATAG TTGCATCTGT CAGTGGTCCA AAAAGGCCTC AGGATAGAGT TGCTGTAACA 
GATATGAAAA GTGATTTTCA GGCTTGCTTA AATGAAAAGG TTGGATTTAA AGGCTTCCAA 
GTTGCAGCAG AAAAACAAAG TGATACTGTC TCAGTTCGTT ATGATGGAAG TGAGTATAAG 
CTGTCCCATG GGTCCGTGGT CATTGCTGCG GTTATCAGCT GTACCAATAA CTGCAATCCA 
TCGGTGATGC TCGCTGCAGG TCTTTTGGCT AAAAAGGCTG TTGAAACTGG TCTACGAGTT 
AAACCTTATA TAAGAACAAG TTTGTCTCCA GGCAGTGGGA TGGTTACACA TTACCTCAGT 
TCAAGTGGAG TGTTACCCTA CCTTAGCAAG CTAGGGTTTG AAATAGTTGG CTATGGATGT 
TCAACGTGTG TAGGAAATAC AGCACCTTTA TCAGAAGCAA TTTTGAATGC AGTAAAACAG 
GGTGATTTGG TTACCTGTGG AGTTTTATCT GGAAACAAAA ATTTCGAAGG TCGTCTTTGT 
GATTGTGTCC GTGCTAATTA TCTCGCCTCT CCACCCTTAG TGGTGGCTTA CGCCATAGCA 
GGCACAGTGA ATATAGATTT CCAGACAGAG CCATTAGGTA CTGACTCTAC AGGCAAGAAC 
ATTTACCTGC ATGACATTTG GCCTAGTCGA GAAGAAGTTC ATCAGATAGA AGAAGAACAT 
GTTATTTTGT CCATGTTTAA AGCACTGAAA GAGAAAGTAG AGATGGGAAA TAAACGGTGG 
AATTCCTTAG ACGCTCCAGA TTCAGTGTTG TTTCCATGGG ATGTTAAGTC TACTTACATC 
AGATGCCCTT CATTTTTTGA TAAACTTACC AAAGAACCAG CTGCATCCCA GCCTATTGAA 
AATGCCCATG TCCTGTTGTA CTTGGGAGAC TCTGTCACTA CAGACCACAT ATCACCTGCT 
GGAAGCATTG CTAGGAGCAG TGCTGCTGCT AAGTACTTGA CAAACAGAGG CCTTACTCCC 
CGAGAGTTCA ACTCTTATGG AGCTCGAAGA GGTAATGATG CTGTGATGAC AAGAGGCACG 
TTTGCAAACA TCAAGCTTTT TAATAAGTTT ATTGGGAAGC CAGCTCCCAA AACAATTCAT 
TTTCCATCAG GACAGACGCT CGATGTATTT GAAGCTGCAG AATTATACCA AAAAGAAGGT 
ATCCCACTGA TAATTTTAGC AGGAAAAAAA TATGGTTCAG GAAATTCAAG AGACTGGGCT 
GCGAAAGGAC CCTATTTGCT GGGTGTAAAG GCTGTTTTGG CTGAAAGCTA TGAAAAGATA 
CACAAAGATC ATTTGATTGG AATTGGTATA GCACCACTTG AGTTCCTCCC AGGAGAAAAT 
GCAGATTCCT TGGGCCTCTC CGGCAGAGAA GTATTTTCTT TATCATTTCC TGAAGAACTC 
TTTCCTGGAA TTACGTTAAA TATAAAGACG AGCACTGGCA AAGAGTTCAG CGTGATTGCA 
GCATTTGAAA ATGATGTGGA GATAACTTTG TACAAACATG GAGGATTGTT AAACTTTGTG 
GCTCGAAAAT TCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243153.2
CDS	229..2946
Translation	MKKEDVINILDWKTKQSNVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELSRNSGTFSSQIENTPVLCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSGAFKNVAVIPPGTGMAHQVNLEHLSRVVFEEADLLFPDSVIGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVTLTLPEVVGCELTGSSNAFVTSIDIVLGITKHLRQVGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLRHLEHTGFDKTKLESMEEYLKAVKLFRNDENSSEPEYSQVIQINLNSIVASVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQVAAEKQSDTVSVRYDGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVETGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEIVGYGCSTCVGNTAPLSEAILNAVKQGDLVTCGVLSGNKNFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFQTEPLGTDSTGKNIYLHDIWPSREEVHQIEEEHVILSMFKALKEKVEMGNKRWNSLDAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVFSLSFPEELFPGITLNIKTSTGKEFSVIAAFENDVEITLYKHGGLLNFVARKFL

Target ORF information:

RefSeq Version	XM_006243091.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iron responsive element binding protein 2 (Ireb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243091.3

ORF Insert Sequence:

ATGAAAAAGG AAGATGTTAT AAATATTTTG GACTGGAAAA CCAAACAAAG CAATGTTGAA 
GTGCCCTTTT TCCCCGCCCG TGTTGTTCTT CAAGATTTCA CTGGAATACC GGCAATGGTG 
GATTTTGCTG CTATGAGGGA GGCAGTGAAA ACTCTTGGAG GTGATCCTAA GAAAGTCCAC 
CCTGCCTGTC CAACAGATCT CACAGTTGAC CACTCTTTAC AGATTGACTT CAGTAAATGT 
GCAATACAGA ATGCACCAAA TCCTGGAGGT GGTGACCTAC AGAAAGCAGG AAAGCTCTCT 
CCACTTAAAG TACAGCCTAA GAAGCTTCCA TGTCGAGGCC AGACTACCTG CCGGGGATCG 
TGTGATTCTG GAGAACTAAG CCGAAACTCA GGAACATTTT CTTCGCAGAT TGAGAATACG 
CCTGTCCTGT GTCCCTTTCA TTTGCAACCA GTGCCTGAAC CTGAGACGGT GTTAAAAAAT 
CAAGAAGTAG AATTTGGCAG AAATCGAGAG AGGCTTCAAT TTTTCAAGTG GAGCTCAGGA 
GCTTTTAAGA ATGTGGCAGT CATCCCTCCT GGAACTGGAA TGGCTCATCA AGTGAACTTA 
GAACATTTGT CGAGAGTAGT GTTTGAAGAA GCCGACCTGC TCTTCCCAGA CAGCGTAATT 
GGCACAGATT CTCATATAAC CATGGTGAAT GGATTGGGAA TTCTTGGGTG GGGAGTTGGA 
GGCATTGAGA CAGAGGCAGT TATGCTTGGC CTGCCAGTTA CTCTTACTTT ACCAGAGGTG 
GTTGGATGTG AGCTAACTGG GTCATCCAAT GCTTTTGTTA CATCCATAGA TATTGTCCTA 
GGCATTACAA AGCACCTCAG GCAAGTAGGC GTGGCTGGAA AGTTTGTTGA GTTCTTTGGA 
AGTGGAGTTT CACAATTATC TATTGTCGAT AGAACTACTA TAGCAAACAT GTGTCCCGAG 
TATGGTGCTA TCCTCAGCTT TTTCCCTGTT GACAATGTGA CACTACGACA TTTAGAACAT 
ACAGGTTTTG ACAAAACCAA ACTTGAGTCA ATGGAAGAAT ACCTTAAAGC TGTGAAATTG 
TTTCGAAATG ATGAGAATTC TTCAGAACCT GAATATTCTC AGGTGATACA GATTAATCTG 
AATTCAATAG TTGCATCTGT CAGTGGTCCA AAAAGGCCTC AGGATAGAGT TGCTGTAACA 
GATATGAAAA GTGATTTTCA GGCTTGCTTA AATGAAAAGG TTGGATTTAA AGGCTTCCAA 
GTTGCAGCAG AAAAACAAAG TGATACTGTC TCAGTTCGTT ATGATGGAAG TGAGTATAAG 
CTGTCCCATG GGTCCGTGGT CATTGCTGCG GTTATCAGCT GTACCAATAA CTGCAATCCA 
TCGGTGATGC TCGCTGCAGG TCTTTTGGCT AAAAAGGCTG TTGAAACTGG TCTACGAGTT 
AAACCTTATA TAAGAACAAG TTTGTCTCCA GGCAGTGGGA TGGTTACACA TTACCTCAGT 
TCAAGTGGAG TGTTACCCTA CCTTAGCAAG CTAGGGTTTG AAATAGTTGG CTATGGATGT 
TCAACGTGTG TAGGAAATAC AGCACCTTTA TCAGAAGCAA TTTTGAATGC AGTAAAACAG 
GGTGATTTGG TTACCTGTGG AGTTTTATCT GGAAACAAAA ATTTCGAAGG TCGTCTTTGT 
GATTGTGTCC GTGCTAATTA TCTCGCCTCT CCACCCTTAG TGGTGGCTTA CGCCATAGCA 
GGCACAGTGA ATATAGATTT CCAGACAGAG CCATTAGGTA CTGACTCTAC AGGCAAGAAC 
ATTTACCTGC ATGACATTTG GCCTAGTCGA GAAGAAGTTC ATCAGATAGA AGAAGAACAT 
GTTATTTTGT CCATGTTTAA AGCACTGAAA GAGAAAGTAG AGATGGGAAA TAAACGGTGG 
AATTCCTTAG ACGCTCCAGA TTCAGTGTTG TTTCCATGGG ATGTTAAGTC TACTTACATC 
AGATGCCCTT CATTTTTTGA TAAACTTACC AAAGAACCAG CTGCATCCCA GCCTATTGAA 
AATGCCCATG TCCTGTTGTA CTTGGGAGAC TCTGTCACTA CAGACCACAT ATCACCTGCT 
GGAAGCATTG CTAGGAGCAG TGCTGCTGCT AAGTACTTGA CAAACAGAGG CCTTACTCCC 
CGAGAGTTCA ACTCTTATGG AGCTCGAAGA GGTAATGATG CTGTGATGAC AAGAGGCACG 
TTTGCAAACA TCAAGCTTTT TAATAAGTTT ATTGGGAAGC CAGCTCCCAA AACAATTCAT 
TTTCCATCAG GACAGACGCT CGATGTATTT GAAGCTGCAG AATTATACCA AAAAGAAGGT 
ATCCCACTGA TAATTTTAGC AGGAAAAAAA TATGGTTCAG GAAATTCAAG AGACTGGGCT 
GCGAAAGGAC CCTATTTGCT GGGTGTAAAG GCTGTTTTGG CTGAAAGCTA TGAAAAGATA 
CACAAAGATC ATTTGATTGG AATTGGTATA GCACCACTTG AGTTCCTCCC AGGAGAAAAT 
GCAGATTCCT TGGGCCTCTC CGGCAGAGAA GTATTTTCTT TATCATTTCC TGAAGAACTC 
TTTCCTGGAA TTACGTTAAA TATAAAGACG AGCACTGGCA AAGAGTTCAG CGTGATTGCA 
GCATTTGAAA ATGATGTGGA GATAACTTTG TACAAACATG GAGGATTGTT AAACTTTGTG 
GCTCGAAAAT TCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypobaric Hypoxia Regulates Brain Iron Homeostasis in Rats.
Journal of cellular biochemistry118(6)1596-1605(2017 Jun)
Li Y,Yu P,Chang SY,Wu Q,Yu P,Xie C,Wu W,Zhao B,Gao G,Chang YZ

Insights into the role of iron in immature rat model of hypoxic-ischemic brain injury.
Experimental and therapeutic medicine12(3)1723-1731(2016 Sep)
Wang ZW,Yang LJ,Ding YX,Chang YZ,Cui H

Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model.
Genes & nutrition10(6)54(2015 Nov)
Zhang Y,Sun X,Xie C,Shu X,Oso AO,Ruan Z,Deng ZY,Wu X,Yin Y

Deficiency of Calcium-Independent Phospholipase A2 Beta Induces Brain Iron Accumulation through Upregulation of Divalent Metal Transporter 1.
PloS one10(10)e0141629(2015)
Beck G,Shinzawa K,Hayakawa H,Baba K,Yasuda T,Sumi-Akamaru H,Tsujimoto Y,Mochizuki H